WO2023087777A1 - 一种芯片检测样本定量注样的系统装置、方法与用途 - Google Patents

一种芯片检测样本定量注样的系统装置、方法与用途 Download PDF

Info

Publication number
WO2023087777A1
WO2023087777A1 PCT/CN2022/108704 CN2022108704W WO2023087777A1 WO 2023087777 A1 WO2023087777 A1 WO 2023087777A1 CN 2022108704 W CN2022108704 W CN 2022108704W WO 2023087777 A1 WO2023087777 A1 WO 2023087777A1
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
sample
injection
quantitative
groove
Prior art date
Application number
PCT/CN2022/108704
Other languages
English (en)
French (fr)
Inventor
杜佩
苏阳
张研
Original Assignee
江苏液滴逻辑生物技术有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 江苏液滴逻辑生物技术有限公司 filed Critical 江苏液滴逻辑生物技术有限公司
Publication of WO2023087777A1 publication Critical patent/WO2023087777A1/zh

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components

Definitions

  • the application belongs to the technical field of microfluidic chips, and relates to quantitative sample injection in the chip, in particular to a system device, method and application for quantitative sample injection of chip detection samples.
  • test sample needs to be added by the test personnel when using the chip after the sample is collected and processed.
  • test sample is collected in the collection tube and needs to be injected into the sample in the digital microfluidic chip.
  • the amount is certain. If liquid quantitative tools such as pipettes and droppers are used to quantitatively remove the liquid from the collection tube and then inject it into the sample inlet of the chip, the user's operation steps will be increased, and the usage scenarios of the digital microfluidic chip will be greatly limited.
  • CN109652298A discloses a droplet PCR amplification detection device based on a microfluidic chip, which includes a droplet microfluidic chip, an XYZ motion unit, a PCR amplification unit, and a detection unit. After the droplets containing DNA molecules are generated, they are introduced into the droplet microfluidic chip through a pipette gun, and the chip is placed in the droplet PCR amplification unit for PCR amplification reaction.
  • CN107831811A discloses a microchannel flow control device and control method for micro-nanocellulose.
  • the device includes: a syringe pump, an injection part, a sample plug, a microfluidic chip, a sealing film, etc., wherein the injection pump quantitatively controls the injection of micro-nano The flow rate of the cellulose suspension; the injection part is used to contain the micro-nano cellulose suspension and inject the micro-nano cellulose suspension into the microfluidic chip.
  • CN112271416A discloses a lithium battery liquid injection device and a manufacturing method that utilizes a liquid storage cup to inject electrolyte, and its structure includes a battery case, a mold support and a liquid storage cup, etc., and a detachable There is a locking assembly connected in the same way. There is a liquid injection chamber connected by the locking assembly to form a sealed liquid injection chamber between the liquid storage cup and the top of the battery case.
  • the liquid storage cup is provided with a liquid storage chamber for quantitatively storing electrolyte.
  • the electrolyte can be pressurized by the external pressure and enter the liquid injection chamber first, and then inject it into the battery case at one time through the liquid injection hole.
  • the digital microfluidic chip adopts the principle of electrowetting technology, which regulates the surface energy of solid and liquid through potential, and utilizes the difference in surface energy.
  • the equilibrium state drives the liquid to move, so as to achieve precise control of the micro-liquid, which relies heavily on the syringe pump, and the cost is high.
  • this application provides a system device, method and application for quantitative injection of chip detection samples.
  • the quantitative extraction of the required detection samples can be completed through the built-in sample quantitative components, without additional Quantitative tools, quantitative injection of test samples, so as to realize the automation of chip injection, reduce the dependence on additional quantitative tools, and make the operation more convenient and flexible.
  • the present application provides a system device for quantitative injection of a chip detection sample, which is used to inject quantitative detection samples into the gap cavity of the chip.
  • the system device includes a sample quantitative component and an injection chamber.
  • the sample quantification assembly includes a body, a quantification chamber is arranged in the body, and the liquid injection chamber includes an open casing, and a partition is arranged in the casing, and the partition separates the casing.
  • the test sample is injected into the first chamber, the sample quantification component is placed in the liquid injection chamber, and continuously pressed down, the body extends into the first chamber for quantification Sample injection.
  • the system device for quantitative sample injection of chip detection samples provided by this application can complete the quantitative extraction of the required detection samples through the built-in sample quantitative components during use, and quantitatively inject the detection samples without additional quantitative tools, thereby Realize the automation of chip injection, reduce the dependence on additional quantitative tools, and make the operation more convenient and flexible.
  • the size of the housing and the body can be adjusted to meet the detection sample volume requirements of different systems, thereby realizing the quantitative injection function of larger or smaller liquid volumes.
  • the first chamber provided in this application plays the role of quantitative sample injection
  • the second chamber plays the role of overflow liquid storage
  • the structure and combination method are not specifically limited or required.
  • the partition adopts a hollow cylinder
  • the housing is divided into a first chamber inside the cylinder and a second chamber outside the cylinder.
  • the first chamber The chamber and the second chamber are contained, and the sampling column is located in the first chamber;
  • the partition adopts a vertical plate
  • the housing is divided into the first chamber and the second chamber arranged side by side, which can be placed in the first chamber
  • a gap is set at the connection with the second chamber, thereby realizing the overflow of the detection sample.
  • the first chamber is provided with at least one sampling column connected to the gap cavity.
  • the sample quantification component is continuously pressed down, and the sampling column extends into the quantitative chamber. .
  • the sample injection column in this application can be integrally formed with the casing, or can be a separate component assembled at the bottom of the casing.
  • the liquid injection chamber further includes a sample injection channel passing through the sample injection column, and the sample injection channel is connected to the gap cavity of the chip.
  • sample injection channel there are no specific limitations or special requirements on the structure of the sample injection channel in this application.
  • straight holes or oblique holes through the sample injection column can be selected, or tubular fittings can be installed in the sample injection column.
  • a guiding notch is provided at one end of the sample injection channel close to the gap cavity.
  • the inner wall of the housing near the open end is provided with a long exhaust slot.
  • the length of the exhaust long groove matches the height of the body, and it should be ensured that when the sample quantification component is pressed down to the bottom of the housing, the exhaust is completed, so that the overflowing liquid is sealed in the second chamber.
  • a seal is provided at one end of the body protruding into the casing.
  • the main body extends into the first chamber and forms a sealed environment with the first chamber through the sealing member, so that the liquid can only flow out from the sample injection channel after being squeezed.
  • a groove is formed on the outer wall of the body, and the sealing member is arranged in the groove.
  • the sealing element is an "O" type sealing ring.
  • the sample quantification component drops slowly and uniformly under the downward pressure.
  • the "O" ring starts to enter the first chamber, the excess detection sample in the first chamber is squeezed by the body. And overflow into the second chamber, and continue to descend until the "O" ring completely enters the first chamber, forming a sealed environment, and the detection sample volume in the first chamber is basically constant.
  • the detection sample enters the injection chamber.
  • the sample channel enters the gap cavity of the chip, and when the body is pressed down to the bottom of the first chamber, the quantitative sample injection is completed.
  • the quantitative component in this application can be pre-stored in the empty area of the chip shell or packaged together with the chip, and placed on the liquid injection chamber after the sample to be tested is initially dropped.
  • the sample quantification assembly further includes a base for fixing the body, and during the sample injection process, the edge of the base is in interference fit with the inner wall of the housing to achieve a seal.
  • the sample quantification component with the seal assembled is placed in the liquid injection chamber, and it must be placed horizontally.
  • the base of the sample quantification component has interference with the inner wall of the housing during the pressing down process, and the To the secondary sealing effect.
  • a guide piece is further provided on the surface of the base near the side of the body.
  • the partition gradually extends into the gap during the sample injection process.
  • the guide in the present application allows the sample quantification component to descend vertically, avoiding the deviation or inclination of the main body, resulting in deviation of the liquid injection, or being stuck and unable to be pressed down.
  • the surface of the base is provided with exhaust holes.
  • an exhaust notch is provided on the outer edge of the base.
  • the sampling column is higher than the partition.
  • the detection sample circulates in the gap.
  • a stepped groove is provided on the side surface of the partition close to the sample quantification component.
  • the stepped groove on the top of the partition serves as a buffer.
  • the step groove is divided into a first groove and a second groove along the direction in which the body extends into the first chamber, the width of the first groove is greater than the width of the second groove, and the detection The sample is located at the junction of the first groove and the second groove.
  • the system device injects the detection sample into the first chamber of the liquid injection chamber before starting the sample injection, and the detection sample drops to the joint between the first groove and the second groove. At this time, because the injection column is higher than the first chamber, the liquid cannot enter the injection channel for the time being.
  • the sample quantification component is pressed down, and part of the detection sample is gradually squeezed into the gap between the injection column and the quantification chamber. Then, it enters the injection channel and flows into the gap cavity.
  • the present application provides a method for quantitative injection of a chip detection sample, the method adopts the system device described in the first aspect, and the method includes:
  • test sample is injected into the first chamber, the sample quantification component is placed in the liquid injection chamber, the sample quantification component is continuously pressed down, the body is sent into the first chamber from the open end of the housing, and part of the test sample in the first chamber overflows.
  • the detection sample entering the quantitative chamber flows into the gap chamber to realize quantitative sample injection.
  • the quantitative sample injection specifically includes:
  • the sample quantitative component is continuously pressed down, and the sample injection column gradually extends into the quantitative chamber, and the detection sample is squeezed into the gap between the sample injection column and the quantitative chamber, and then flows into the gap cavity through the sample injection channel.
  • the method includes using a collection tube to inject a test sample into the first chamber.
  • the pressing down of the body includes using a motor or a lifting mechanism to realize automatic pressing down.
  • the method further includes degassing during the quantitative sample injection process.
  • the exhaust is carried out by using the exhaust through holes opened on the surface of the base.
  • an exhaust notch is provided on the outer edge of the base for exhaust.
  • exhaust is carried out by using long exhaust slots on the inner wall of the casing.
  • a method for quantitative injection of a chip detection sample specifically includes the following steps:
  • the present application provides an application of the system device described in the first aspect, and the system device is used for injecting a detection sample into a gap cavity of a digital microfluidic chip.
  • the digital microfluidic chip in this application adopts the principle of electrowetting technology, regulates the surface energy of solid and liquid through potential, and uses the unbalanced state of surface energy to drive the liquid to move, so as to achieve accurate microfluidics. manipulation.
  • the main components include: a transparent conductive cover (such as ITO glass), an electrode array with a hydrophobic layer and a dielectric layer on the surface, and a gap cavity for droplet movement between the transparent conductive cover and the electrode array.
  • the surface of the electrode array is provided with the chip detection sample quantitative injection system device provided by the application, and the sample injection column communicates with the gap cavity through the sample injection channel.
  • the system refers to an equipment system, a device system or a production device.
  • the application provides a system device, method and application for quantitative sample injection of chip detection samples.
  • the quantitative extraction of the required detection samples can be completed through the built-in sample quantitative components, without additional quantitative tools.
  • FIG. 1 is a schematic structural diagram of a system device for quantitative sample injection of a chip detection sample provided in Example 1 of the present application;
  • FIG. 2 is a schematic structural diagram of the sample quantitative component provided in Example 1 of the present application.
  • FIG. 3 is a schematic structural diagram of the liquid injection chamber provided in Example 1 of the present application.
  • FIG. 4 is a schematic diagram of the sample quantification component provided in Example 1 of the present application entering the liquid injection chamber;
  • FIG. 5 is a schematic structural diagram of the digital microfluidic chip provided in Application Example 1 of the present application.
  • FIG. 6 is a schematic flow chart of quantitative sample injection provided in Application Example 1 of the present application.
  • 1-sample quantitative component 2-injection chamber; 3-body; 4-"O" type sealing ring; 5-quantitative chamber; 6-guide; 7-housing; 8-baffle; 9 -first chamber; 10-second chamber; 11-injection column; 12-injection channel; 13-step groove; 14-gap cavity; 15-electrode array; 16-hydrophobic layer; 17-dielectric layer ;18-Transparent conductive cover.
  • the present application provides a system device for quantitative injection of a chip detection sample.
  • the system device is used to inject a quantitative detection sample into the gap cavity 14 of the chip.
  • the system device includes a sample Quantitative assembly 1 and liquid injection chamber 2, described sample quantitative assembly 1 comprises body 3, and described body 3 is provided with quantitative chamber 5, and described liquid injection chamber 2 comprises open casing 7, and
  • the casing 7 is provided with a partition 8, and the partition 8 divides the casing 7 into a first chamber 9 and a second chamber 10, the first chamber 9 is injected with a detection sample, and the The sample quantitative component 1 is placed in the liquid injection chamber 2 and continuously pressed down, the body 3 protrudes into the first chamber 9 for quantitative sample injection.
  • the sample volume requirements of different systems can be realized by adjusting the size of the housing 7 and the body 3, thereby realizing the quantitative sample injection function of larger or smaller liquid volumes.
  • the first chamber 9 provided in this application plays the role of quantitative sample injection
  • the second chamber 10 plays the role of overflow liquid storage;
  • the structure and combination are not specifically limited or required.
  • the partition 8 is a hollow cylinder
  • the housing 7 is divided into a first chamber 9 inside the cylinder and a second chamber 10 outside the cylinder.
  • the first chamber 9 and the second chamber 10 are in an inclusive relationship, and the sampling column 11 is located in the first chamber 9;
  • the partition board 8 adopts a vertical plate the housing 7 is divided into the first chamber 9 arranged side by side.
  • a gap can be provided at the connection between the first chamber 9 and the second chamber 10, so as to realize the overflow of the detection sample.
  • the first chamber 9 is provided with at least one sampling column 11 communicating with the gap cavity 14.
  • the sample quantification component 1 is continuously pressed down, and the sampling column 11 extends into the quantitative cavity. Room 5.
  • the sampling column 11 in this application can be integrally formed with the casing 7 , or can be a separate component assembled at the bottom of the casing 7 .
  • the liquid injection chamber 2 also includes a sample injection channel 12 passing through the sample injection column 11 , and the sample injection channel 12 is connected to the gap cavity 14 of the chip.
  • a sample injection channel 12 passing through the sample injection column 11 , and the sample injection channel 12 is connected to the gap cavity 14 of the chip.
  • a straight hole or an inclined hole through the sample injection column 11 can be selected, or tubular fittings can be installed in the sample injection column 11 .
  • One end of the sample injection channel 12 close to the gap cavity 14 is provided with a guide notch.
  • the inner wall of the housing 7 near the open end is provided with a long exhaust slot.
  • the length of the exhaust long groove matches the height of the body 3, and it should be ensured that when the sample quantification component 1 is pressed down to the bottom of the housing 7, the exhaust is completed, so that the overflowing liquid is sealed in the second chamber 10.
  • a seal is provided at one end of the body 3 protruding into the casing 7 .
  • the body 3 protrudes into the first chamber 9 and forms a sealed environment with the first chamber 9 through the sealing member, so that the liquid can only flow out from the sample injection channel 12 after being squeezed.
  • a groove is defined on the outer wall of the body 3, and the sealing element is disposed in the groove.
  • the sealing member is an "O" type sealing ring 4.
  • the sample quantification component 1 is slowly and uniformly descended under the downward pressure.
  • the main body 3 squeezes into the second chamber 10, and continues to descend until the "O” ring starts to seal once, the liquid volume in the first chamber 9 is basically constant, and as the pressure continues, the sample enters the chip through the injection column 11.
  • the quantitative component in this application can be pre-stored in the empty area of the chip shell or packaged together with the chip, and placed on the liquid injection chamber after the sample to be tested is initially dropped.
  • the sample quantification assembly 1 further includes a base for fixing the body 3 , and during the sample injection process, the edge of the base is in interference fit with the inner wall of the casing 7 to achieve sealing.
  • the sample quantitative assembly 1 with the seal assembled is placed in the liquid injection chamber 2, and it needs to be placed horizontally.
  • the base of the sample quantitative assembly 1 has an interference with the inner wall of the housing 7 during the pressing process, which plays a role Secondary sealing effect.
  • a guide piece 6 is also provided on the surface of the base near the side of the body 3 . There is a gap between the body 3 and the guide 6, and the partition 8 gradually extends into the gap during the sample injection process.
  • the guide 6 in this application allows the sample quantification component 1 to descend vertically, avoiding the deviation or inclination of the main body 3 to cause deviation in liquid injection, or jamming and failure to press down.
  • the surface of the base is provided with exhaust holes.
  • the outer edge of the base is provided with an exhaust notch.
  • sampling column 11 is higher than the partition 8 .
  • a stepped groove 13 is provided on the side surface of the partition plate 8 close to the sample quantitative assembly 1 .
  • the step groove 13 is divided into a first groove and a second groove along the direction in which the body 3 extends into the first chamber 9, the width of the first groove is greater than the width of the second groove, and the detection The sample is located at the junction of the first groove and the second groove.
  • the system device provided by the present application injects the detection sample into the first chamber 9 of the liquid injection chamber 2 before starting the sample injection, and the detection sample drops to the joint between the first groove and the second groove.
  • the injection column 11 is higher than the first chamber 9, and the liquid cannot enter the injection channel 12 for the time being.
  • the sample quantification component 1 is pressed down, and part of the detection sample is gradually squeezed into the injection column 11 and quantified. Between the chambers 5 , it enters the sample injection channel 12 and flows into the gap cavity 14 .
  • the present application provides a method for quantitative injection of a chip detection sample.
  • the method uses the system device described in the first aspect, and the method includes:
  • the quantitative sample injection specifically includes:
  • the method includes using a collection tube to inject a detection sample into the first chamber 9 .
  • the detection sample is located at the junction of the first groove and the second groove.
  • the pressing down body 3 includes a motor or a lifting mechanism to realize automatic pressing down.
  • the method also includes exhausting during the quantitative sample injection process.
  • Exhaust is carried out through the exhaust through hole opened on the surface of the base.
  • the outer edge of the base is provided with an exhaust notch for exhaust.
  • Exhaust is carried out through the long exhaust grooves on the inner wall of the housing 7 .
  • This embodiment provides a system device for quantitative sample injection of a chip detection sample, as shown in Figure 1, the system device includes a sample quantitative component 1 and a liquid injection chamber 2, as shown in Figure 2, the sample quantitative component 1 includes a body 3, the body 3 is provided with a quantitative chamber 5, the outer wall of the body 3 is provided with a groove, and an "O"-shaped sealing ring 4 is arranged in the groove.
  • the sample quantification component 1 also includes a base for fixing the body 3. During the sample injection process, the edge of the base and the inner wall of the housing 7 are in an interference fit to achieve sealing.
  • the side surface of the base close to the body 3 is also provided with four guides. 6. There is a gap between the body 3 and the guide 6.
  • the liquid injection chamber 2 includes an open housing 7, the housing 7 is provided with a partition 8, the partition 8 is a hollow cylinder, and the partition 8 divides the housing 7 into the first chamber 9 and the second chamber 10 , a sampling column 11 communicating with the gap cavity 14 is arranged in the first chamber 9 .
  • the liquid injection chamber 2 also includes a sample injection channel 12 passing through the sample injection column 11, the sample injection channel 12 is connected to the gap cavity 14 of the chip, and one end of the sample injection channel 12 near the gap cavity 14 is provided with a guide gap, as shown in Figure 3,
  • the inner wall of the housing 7 near the open end is provided with a long exhaust slot.
  • the sampling column 11 is higher than the partition 8 , and there is a gap between the outer wall of the sampling column 11 and the inner wall of the quantitative chamber 5 , and the detection sample circulates in the gap.
  • the side surface of the partition plate 8 close to the sample quantitative assembly 1 is provided with a stepped groove 13, and the stepped groove 13 is divided into a first groove and a second groove along the direction in which the body 3 extends into the first chamber 9, and the first groove The width is greater than the width of the second groove, and the detection sample is located at the junction of the first groove and the second groove.
  • the chip detection sample quantitative injection system device provided in Example 1 is used to inject reagents into the gap cavity 14 of the digital microfluidic chip as shown in Figure 5.
  • the digital microfluidic chip in this application example mainly The composition includes: a transparent conductive cover 18, an electrode array 15 with a hydrophobic layer 16 and a dielectric layer 17 on the surface, and a gap cavity 14 for droplet movement between the transparent conductive cover 18 and the electrode array 15.
  • the surface of the electrode array 15 is provided with the chip detection sample quantitative injection system device provided by the present application, and the sample injection column 11 communicates with the gap cavity 14 through the sample injection channel 12 .
  • the liquid injection chamber 2 is placed directly under the pressure head of the press;
  • serial number Pressure value (N) serial number Pressure value (N) 1 152.3 11 138.4 2 136.1 12 144.7 3 158.7 13 145.1 4 156.6 14 150.4 5 150.3 15 153.9 6 162.4 16 141.5 7 139.5 17 134.7 8 143.4 18 136.6 9 162.3 19 131.8 10 141.2 20 139.6
  • the range of the test samples of the forty sets of data is 12.8 ⁇ L, and the injection accuracy meets the requirements.
  • the sample quantification component 1 provided by this application has good sealing performance, and the test samples can be injected into the chip from the sample injection channel 12 as expected. Interstitial chamber 14. It can be known from Table 2 that the average pressure required by the sample quantification component 1 is 145.975N, the maximum pressure value is 158.7N, and the minimum pressure value is 131.8N.
  • the system device for quantitative injection of chip detection samples provided by this application can complete the quantitative extraction of the required detection samples through the built-in sample quantitative component 1 during use, and quantitatively inject the detection samples without additional quantitative tools, thereby realizing The automation of chip injection reduces the dependence on additional quantitative tools, making the operation more convenient and flexible.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

一种芯片检测样本定量注样的系统装置、方法与用途，该系统装置用于将定量的检测样本注入芯片的间隙腔（14）内，该系统装置包括样本定量组件（1）与注液腔室（2），样本定量组件（1）包括本体（3），本体（3）内设有定量腔室（5），注液腔室（2）包括敞口的壳体（7），壳体（7）内设有隔板（8），隔板（8）将壳体（7）分为第一腔室（9）与第二腔室（10），第一腔室（9）内注入检测样本，将样本定量组件（1）置于注液腔室（2），并持续下压，本体（3）伸入第一腔室（9）内进行定量注样。

Description

一种芯片检测样本定量注样的系统装置、方法与用途 技术领域
本申请属于微流控芯片技术领域，涉及芯片内的定量注样，尤其涉及一种芯片检测样本定量注样的系统装置、方法与用途。
背景技术
在聚合酶链式反应(PCR)反应过程中，检测样本需要在样本采集处理后，由检测人员在使用芯片时添加，通常检测样本收集在采集管中，需注入数字微流控芯片中的样本量是一定的，若通过移液器、滴管等液体定量工具从采集管中定量取出再注入芯片样本入口，增加用户的操作步骤，且使数字微流控芯片的使用场景受到极大局限。
CN109652298A公开了一种基于微流控芯片的液滴PCR扩增检测装置，该装置包括液滴微流控芯片、XYZ运动单元、PCR扩增单元、检测单元。含有DNA分子的液滴生成后，通过移液枪导入液滴微流控芯片中，将芯片置于液滴PCR扩增单元中进行PCR扩增反应。
CN107831811A公开了一种微纳米纤维素的微流道流动控制装置及控制方法，装置包括：注射泵、注射部分、加样塞、微流控芯片、密封膜等，其中注射泵定量控制注射微纳米纤维素悬浮液的流量大小；注射部分用于包含有微纳米纤维素的悬浮液并将微纳米纤维素悬浮液注射入微流控芯片内。
CN112271416A公开了一种利用储液杯注入电解液的锂电池注液装置及制造方法，其结构包括电池壳体、模托和储液杯等，该储液杯与模托之间设有可拆式连接的锁定组件，储液杯与电池壳体顶部之间设有受锁定组件连接而形成密封的注液腔，储液杯内设有定量储存电解液的储液腔，该储液腔内的电解液 能受外界压力加压先进入注液腔，再经注液孔一次性注入电池壳体内。
传统的PCR反应一旦启动无法在中途新增反应组，全手动式操作耗时耗力，数字微流控芯片采用电润湿技术原理，通过电势调控固、液表面能，并利用表面能的不平衡状态驱动液体产生移动，从而达到对微液体的精确操控，极大地依赖于注射泵，成本较高。
发明内容
针对现有技术存在的不足，本申请提供一种芯片检测样本定量注样的系统装置、方法与用途，使用过程中可通过自带的样本定量组件完成对所需检测样本的定量提取，无需额外定量工具，对检测样本进行定量注入，从而实现芯片注样的自动化，减少对额外定量工具的依赖，使得操作更便捷灵活。
本申请采用以下技术方案：
第一方面，本申请提供了一种芯片检测样本定量注样的系统装置，用于将定量的检测样本注入芯片的间隙腔内，所述的系统装置包括样本定量组件与注液腔室，所述的样本定量组件包括本体，所述本体内设有定量腔室，所述的注液腔室包括敞口的壳体，所述壳体内设有隔板，所述的隔板将壳体分为第一腔室与第二腔室，所述第一腔室内注入检测样本，将所述样本定量组件置于注液腔室，并持续下压，所述本体伸入第一腔室内进行定量注样。
本申请提供的一种芯片检测样本定量注样的系统装置在使用过程中可通过自带的样本定量组件完成对所需检测样本的定量提取，无需额外定量工具，对检测样本进行定量注入，从而实现芯片注样的自动化，减少对额外定量工具的依赖，使得操作更便捷灵活。
本申请中可通过调整壳体和本体的尺寸以满足不同体系的检测样本量需 求，从而实现更大或更小液体量的定量注样功能。
需要说明的是，本申请中提供的第一腔室起到定量注样的作用，第二腔室起到溢流液存储的作用；本申请对于隔板、第一腔室与第二腔室的结构及组合方式不作具体限定或特殊要求，示例性地，当隔板采用空心的柱体，则壳体分为柱体内的第一腔室与柱体外的第二腔室，其中第一腔室与第二腔室为包含关系，进样柱位于第一腔室内；当隔板采用竖立板，则壳体分为并排设置的第一腔室与第二腔室，可在第一腔室与第二腔室连接处设置缺口，进而实现检测样本的溢流。
作为本申请一个优选技术方案，所述第一腔室内设置有连通间隙腔的至少一个进样柱，在定量注样过程中，持续下压样本定量组件，所述进样柱伸入定量腔室内。
本申请中的进样柱可以与壳体一体成型，也可以是单独的构件装配在壳体底部。
优选地，所述的注液腔室还包括贯通所述进样柱的注样通道，所述的注样通道连接芯片的间隙腔。
需要说明的是，本申请中对注样通道的结构不作具体限定或特殊要求，示例性地，可以选择采用贯通进样柱的直孔或斜孔，或者在进样柱内装配管状配件等。
优选地，所述注样通道靠近间隙腔的一端设有导向缺口。
优选地，所述壳体靠近敞口端的内壁设有排气长槽。
需要说明的是，本申请中排气长槽的长度与本体高度相匹配，应保证样本定量组件下压至壳体底部时，排气结束，从而溢出的液体被密封在第二腔室内。
作为本申请一个优选技术方案，所述本体伸入壳体的一端设置密封件。
需要说明的是，在定量注样过程中，本体伸入第一腔室内，并通过密封件与第一腔室形成密封环境，使得液体被挤压后只能从注样通道流出。
优选地，所述本体的外壁开设凹槽，所述密封件设置于所述的凹槽内。
优选地，所述的密封件为“O”型密封圈。
需要说明的是，本申请中注样开始时，样本定量组件受到下压力缓慢匀速下降，当“O”型圈开始进入第一腔室时，第一腔室内多余的检测样本被本体挤压，并溢流至第二腔室内，继续下降至“O”型圈完全进入第一腔室时，形成密封环境，第一腔室内的检测样本量基本恒定，随着持续下压，检测样本进入注样通道并进入芯片间隙腔内，本体下压至第一腔室底部时，完成定量注样。
本申请中的本定量组件可预先存放在芯片壳空置区或随芯片一起包装，待检测样本初步滴入后放置于注液腔室上。
作为本申请一个优选技术方案，所述的样本定量组件还包括用于固定所述本体的底座，在注样过程中，所述底座的边缘与壳体的内壁过盈配合实现密封。
需要说明的是，本申请中将装配好密封件的样本定量组件放置于注液腔室中，需确保水平放置，样本定量组件的底座在下压过程中与壳体的内壁有过盈量，起到二次密封作用。
优选地，所述底座靠近所述本体一侧的表面还设有导向件。
优选地，所述本体与导向件之间留有空隙，在注样过程中，所述隔板逐渐伸入所述空隙中。
需要说明的是，本申请中的导向件使得样本定量组件垂直下降，避免本体发生偏移或倾斜导致注液出现偏差，或卡住无法下压的情况。
优选地，所述底座表面开设有排气通孔。
优选地，所述底座外缘设有排气缺口。
需要说明的是，本领域技术人员可根据具体情况，选择在底座表面开设有排气通孔和/或底座外缘设有排气缺口的方式进行排气。
作为本申请一个优选技术方案，所述进样柱高于所述隔板。
优选地，所述进样柱的外壁与所述定量腔室的内壁之间留有间隙，检测样本在所述间隙内流通。
作为本申请一个优选技术方案，所述隔板靠近样本定量组件的一侧表面开设有台阶槽。
需要说明的是，本申请中隔板顶部的台阶槽起到缓冲的作用。
优选地，所述台阶槽沿所述本体伸入第一腔室的方向分为第一凹槽与第二凹槽，所述第一凹槽的宽度大于所述第二凹槽的宽度，检测样本位于所述第一凹槽与第二凹槽的对接处。
需要说明的是，本申请提供的系统装置在开始注样前，将检测样本注入注液腔室的第一腔室内，检测样本量滴至第一凹槽与第二凹槽的对接处，此时由于进样柱高于第一腔室，液体暂无法进入注样通道，随后进行注样过程中，样本定量组件下压，部分的检测样本逐渐被挤压至进样柱与定量腔室之间，再进入注样通道，并流入间隙腔中。
第二方面，本申请提供了一种芯片检测样本定量注样的方法，所述的方法采用第一方面所述的系统装置，所述的方法包括：
第一腔室内注入检测样本，将样本定量组件置于注液腔室内，持续下压样本定量组件，本体由壳体的敞口端送入第一腔室内，第一腔室内的部分检测样 本溢流至第二腔室中，进入定量腔室内的检测样本流入间隙腔中实现定量注样。
作为本申请一个优选技术方案，所述的定量注样具体包括：
持续下压样本定量组件，所述进样柱逐渐伸入定量腔室内，将检测样本挤入进样柱与定量腔室的间隙，随后通过注样通道流入间隙腔中。
优选地，所述的方法包括利用采集管向第一腔室内注入检测样本。
优选地，所述下压本体包括采用电机或升降机构实现自动化下压。
作为本申请一个优选技术方案，所述的方法还包括在定量注样过程中进行排气。
优选地，采用底座表面开设的排气通孔进行排气。
优选地，采用底座外缘设有排气缺口进行排气。
优选地，采用壳体内壁的排气长槽进行排气。
示例性地，本申请提供的一种芯片检测样本定量注样的方法，具体包括以下步骤：
(1)利用采集管将检测样本滴入第一腔室内，直至检测样本的液面上升至隔板顶部的第一凹槽与第二凹槽的对接处，此时由于进样柱高于隔板，检测样本液体暂无法进入注样通道内；
(2)滴完检测样本，将装配好“O”型圈的样本定量组件水平放置于注液腔室内，其中本体靠近进样柱放置；
(3)利用电机或升降机构下压样本定量组件，使其缓慢匀速下降，采用壳体内壁的排气长槽进行排气，下降到“O”型圈开始进入第一腔室时，底座的边缘与壳体的内壁过盈配合实现密封，第一腔室内多余的检测样本被本体挤压，并溢流至第二腔室内，继续下降至“O”型圈完全进入第一腔室时，形成密封 环境，隔板逐渐伸入本体与导向件之间的空隙中，第一腔室内的检测样本量基本恒定，随着持续下压，检测样本进入注样通道并进入芯片间隙腔内，本体下压至第一腔室底部时，完成定量注样。
第三方面，本申请提供了一种第一方面所述的系统装置的用途，所述的系统装置用于向数字微流控芯片的间隙腔内注入检测样本。
本申请中的数字微流控芯片数字微流控芯片采用电润湿技术原理，通过电势调控固、液表面能，并利用表面能的不平衡状态驱动液体产生移动，从而达到对微液体的精确操控。主要组成包括：透明导电盖子(例如ITO玻璃)、表面包含疏水层和介电层的电极阵列，透明导电盖子和电极阵列之间具有用于液滴移动的间隙腔。电极阵列的表面设置有本申请提供的芯片检测样本定量注样的系统装置，进样柱通过注样通道与间隙腔连通。
所述系统是指设备系统、装置系统或生产装置。
与现有技术相比，本申请的有益效果为：
本申请提供的一种芯片检测样本定量注样的系统装置、方法与用途，在使用过程中可通过自带的样本定量组件完成对所需检测样本的定量提取，无需额外定量工具，对检测样本进行定量注入，从而实现芯片注样的自动化，减少对额外定量工具的依赖，使得操作更便捷灵活。
附图说明
图1为本申请实施例1提供的芯片检测样本定量注样的系统装置的结构示意图；
图2为本申请实施例1提供的样本定量组件的结构示意图；
图3为本申请实施例1提供的注液腔室的结构示意图；
图4为本申请实施例1提供的样本定量组件进入注液腔室的示意图；
图5为本申请应用例1提供的数字微流控芯片的结构示意图；
图6为本申请应用例1提供的定量注样的流程示意图。
其中，1-样本定量组件；2-注液腔室；3-本体；4-“O”型密封圈；5-定量腔室；6-导向件；7-壳体；8-隔板；9-第一腔室；10-第二腔室；11-进样柱；12-注样通道；13-台阶槽；14-间隙腔；15-电极阵列；16-疏水层；17-介电层；18-透明导电盖子。
具体实施方式
需要理解的是，在本申请的描述中，术语“中心”、“纵向”、“横向”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本申请和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本申请的限制。此外，术语“第一”、“第二”等仅用于描述目的，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量。由此，限定有“第一”、“第二”等的特征可以明示或者隐含地包括一个或者更多个该特征。在本申请的描述中，除非另有说明，“多个”的含义是两个或两个以上。
需要说明的是，在本申请的描述中，除非另有明确的规定和限定，术语“设置”、“相连”、“连接”应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或一体连接；可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通。对于本领域 的普通技术人员而言，可以通过具体情况理解上述术语在本申请中的具体含义。
下面结合附图并通过具体实施方式来进一步说明本申请的技术方案。
在一个具体实施方式中，本申请提供了一种芯片检测样本定量注样的系统装置，所述的系统装置用于将定量的检测样本注入芯片的间隙腔14内，所述的系统装置包括样本定量组件1与注液腔室2，所述的样本定量组件1包括本体3，所述本体3内设有定量腔室5，所述的注液腔室2包括敞口的壳体7，所述壳体7内设有隔板8，所述的隔板8将壳体7分为第一腔室9与第二腔室10，所述第一腔室9内注入检测样本，将所述样本定量组件1置于注液腔室2，并持续下压，所述本体3伸入第一腔室9内进行定量注样。
本申请中可通过调整壳体7和本体3的尺寸实现不同体系的样本量需求，从而实现更大或更小液体量的定量注样功能。
本申请中提供的第一腔室9起到定量注样的作用，第二腔室10起到溢流液存储的作用；本申请对于隔板8、第一腔室9与第二腔室10的结构及组合方式不作具体限定或特殊要求，示例性地，当隔板8采用空心的柱体，则壳体7分为柱体内的第一腔室9与柱体外的第二腔室10，其中第一腔室9与第二腔室10为包含关系，进样柱11位于第一腔室9内；当当隔板8采用竖立板，则壳体7分为并排设置的第一腔室9与第二腔室10，可在第一腔室9与第二腔室10连接处设置缺口，进而实现检测样本的溢流。
进一步地，所述第一腔室9内设置有连通间隙腔14的至少一个进样柱11，在定量注样过程中，持续下压样本定量组件1，所述进样柱11伸入定量腔室5内。本申请中的进样柱11可以与壳体7一体成型，也可以是单独的构件装配在壳体7底部。
所述的注液腔室2还包括贯通所述进样柱11的注样通道12，所述的注样通道12连接芯片的间隙腔14。本申请中对注样通道12的结构不作具体限定或特殊要求，示例性地，可以选择采用贯通进样柱11的直孔或斜孔，或者在进样柱11内装配管状配件等。
所述注样通道12靠近间隙腔14的一端设有导向缺口。
所述壳体7靠近敞口端的内壁设有排气长槽。本申请中排气长槽的长度与本体3高度相匹配，应保证样本定量组件1下压至壳体7底部时，排气结束，从而溢出的液体被密封在第二腔室10内。
进一步地，所述本体3伸入壳体7的一端设置密封件。在定量注样过程中，本体3伸入第一腔室9内，并通过密封件与第一腔室9形成密封环境，使得液体被挤压后只能从注样通道12流出。
所述本体3的外壁开设凹槽，所述密封件设置于所述的凹槽内。
所述的密封件为“O”型密封圈4。本申请中注样开始时，样本定量组件1受到下压力缓慢匀速下降，起初下降到“O”型圈还未起到密封作用时，第一腔室9中多余的液体被样本定量组件1的本体3挤入第二腔室10内，继续下降至“O”型圈开始一次密封时，第一腔室9的液体量基本恒定，随着继续下压，样本通过进样柱11进入芯片的间隙腔14，样本定量组件1下压到底时，完成定量注样。
本申请中的本定量组件可预先存放在芯片壳空置区或随芯片一起包装，待检测样本初步滴入后放置于注液腔室上。
进一步地，所述的样本定量组件1还包括用于固定所述本体3的底座，在注样过程中，所述底座的边缘与壳体7的内壁过盈配合实现密封。本申请中将 装配好密封件的样本定量组件1放置于注液腔室2中，需确保水平放置，样本定量组件1的底座在下压过程中与壳体7的内壁有过盈量，起到二次密封作用。
所述底座靠近所述本体3一侧的表面还设有导向件6。所述本体3与导向件6之间留有空隙，在注样过程中，所述隔板8逐渐伸入所述空隙中。本申请中的导向件6使得样本定量组件1垂直下降，避免本体3发生偏移或倾斜导致注液出现偏差，或卡住无法下压的情况。
所述底座表面开设有排气通孔。
所述底座外缘设有排气缺口。
进一步地，所述的进样柱11高于所述隔板8。
所述进样柱11的外壁与所述定量腔室5的内壁之间留有间隙，检测样本在所述间隙内流通。
进一步地，所述隔板8靠近样本定量组件1的一侧表面开设有台阶槽13。所述台阶槽13沿所述本体3伸入第一腔室9的方向分为第一凹槽与第二凹槽，所述第一凹槽的宽度大于所述第二凹槽的宽度，检测样本位于所述第一凹槽与第二凹槽的对接处。
本申请提供的系统装置在开始注样前，将检测样本注入注液腔室2的第一腔室9内，检测样本量滴至第一凹槽与第二凹槽的对接处，此时由于进样柱11高于第一腔室9，液体暂无法进入注样通道12，随后进行注样过程中，样本定量组件1下压，部分的检测样本逐渐被挤压至进样柱11与定量腔室5之间，再进入注样通道12，并流入间隙腔14中。
在另一个具体实施方式中，本申请提供了一种芯片检测样本定量注样的方法，所述的方法采用第一方面所述的系统装置，所述的方法包括：
第一腔室9内注入检测样本，将样本定量组件1置于注液腔室2内，持续下压样本定量组件1，本体3由壳体7的敞口端送入第一腔室9内，第一腔室9内的部分检测样本溢流至第二腔室10中，进入定量腔室5内的检测样本流入间隙腔14中实现定量注样。
进一步地，所述的定量注样具体包括：
持续下压样本定量组件1，所述进样柱11逐渐伸入定量腔室5内，将检测样本挤入进样柱11与定量腔室5的间隙，随后通过注样通道12流入间隙腔14中
所述的方法包括利用采集管向第一腔室9内注入检测样本。检测样本位于所述第一凹槽与第二凹槽的对接处。
所述下压本体3包括采用电机或升降机构实现自动化下压。
进一步地，所述的方法还包括在定量注样过程中进行排气。
采用底座表面开设的排气通孔进行排气。
采用底座外缘设有排气缺口进行排气。
采用壳体7内壁的排气长槽进行排气。
实施例1
本实施例中提供了一种芯片检测样本定量注样的系统装置，如图1所示，所述的系统装置包括样本定量组件1与注液腔室2，如图2所示，样本定量组件1包括本体3，本体3内设有定量腔室5，本体3的外壁开设凹槽，凹槽内设置“O”型密封圈4。样本定量组件1还包括用于固定本体3的底座，在注样过程中，底座的边缘与壳体7的内壁过盈配合实现密封，底座靠近本体3的一侧表面还设有四个导向件6，本体3与导向件6之间留有空隙。
如图3所示，注液腔室2包括敞口的壳体7，壳体7内设有隔板8，隔板8为空心柱体，隔板8将壳体7分为第一腔室9与第二腔室10，第一腔室9内设置有连通间隙腔14的一个进样柱11。注液腔室2还包括贯通进样柱11的注样通道12，注样通道12连接芯片的间隙腔14，注样通道12靠近间隙腔14的一端设有导向缺口，如图3所示，壳体7靠近敞口端的内壁设有排气长槽。
如图4所示，进样柱11高于隔板8，进样柱11的外壁与定量腔室5的内壁留有间隙，检测样本在间隙内流通。
隔板8靠近样本定量组件1的一侧表面开设有台阶槽13，台阶槽13沿本体3伸入第一腔室9的方向分为第一凹槽与第二凹槽，第一凹槽的宽度大于第二凹槽的宽度，检测样本位于第一凹槽与第二凹槽的对接处。
应用例1
本应用例中采用实施例1提供的芯片检测样本定量注样的系统装置向如图5所示的数字微流控芯片的间隙腔14内注入试剂，本应用例中的数字微流控芯片主要组成包括：透明导电盖子18、表面包含疏水层16和介电层17的电极阵列15，透明导电盖子18和电极阵列15之间具有用于液滴移动的间隙腔14。电极阵列15的表面设置有本申请提供的芯片检测样本定量注样的系统装置，进样柱11通过注样通道12与间隙腔14连通。
如图6所示，定量注样具体包括以下步骤：
(1)利用采集管将检测样本滴入第一腔室9内，直至检测样本的液面上升至隔板8顶部的第一凹槽与第二凹槽的对接处，此时由于进样柱11高于隔板8，检测样本液体暂无法进入注样通道12内；
(2)滴完检测样本，将装配好“O”型圈的样本定量组件1水平放置于注 液腔室2内，其中本体3靠近进样柱11放置；
(3)利用电机或升降机构下压样本定量组件1，使其缓慢匀速下降，采用壳体7内壁的排气长槽进行排气，下降到“O”型圈开始进入第一腔室9时，底座的边缘与壳体7的内壁过盈配合实现密封，导向件6伸入第二腔室10内，第一腔室9内多余的检测样本被本体3挤压，并溢流至第二腔室10内，继续下降至“O”型圈完全进入第一腔室9时，形成密封环境，隔板8逐渐伸入本体3与导向件6之间的空隙中，第一腔室9内的检测样本量基本恒定，随着持续下压，检测样本进入注样通道12并进入芯片间隙腔14内，本体3下压至第一腔室9底部时，完成定量注样。
(1)样本定量组件1的密封性与注液精度测试，具体采用以下步骤：
1、将EP管称重，标注序号，记录重量；
2、测量单体及样本定量组件1关键尺寸并标注序号；
3、用移液枪向样本定量组件1注入液体，然后上方放置装配好O型圈的样本定量塞，下方用EP管接注出的液体，手动下压，对接液的EP管称重，记录数据，并计算差值，结果如表1所示；
4、实验结束，整理实验台面。
表1
Figure PCTCN2022108704-appb-000001
Figure PCTCN2022108704-appb-000002
(2)样本定量组件1的压力测试，具体采用以下步骤：
1、注液腔室2放置在压力机压头正下方；
2、在注液腔室2中注入样本液体至台阶槽13附近(液面位于第一凹槽与第二凹槽的对接处)；
2、放置装配好O型圈的样本定量组件1放置于注液腔室2上方；
3、启动压力机下压；
4、观察记录下压时压力值，结果如表2所示。
表2
序号 压力值(N) 序号 压力值(N)
1 152.3 11 138.4
2 136.1 12 144.7
3 158.7 13 145.1
4 156.6 14 150.4
5 150.3 15 153.9
6 162.4 16 141.5
7 139.5 17 134.7
8 143.4 18 136.6
9 162.3 19 131.8
10 141.2 20 139.6
由表1可知，四十组数据测试样本极差为12.8μL，注液精度满足要求，本申请提供的样本定量组件1具有良好的密封性，测试样本可按设想从注样通道12注入芯片的间隙腔14。由表2可知，样本定量组件1所需下压力平均值为：145.975N，最大压力值：158.7N，最小压力值：131.8N。
本申请提供的芯片检测样本定量注样的系统装置在使用过程中可通过自带的样本定量组件1完成对所需检测样本的定量提取，无需额外定量工具，对检测样本进行定量注入，从而实现芯片注样的自动化，减少对额外定量工具的依赖，使得操作更便捷灵活。
申请人声明，以上所述仅为本申请的具体实施方式，但本申请的保护范围并不局限于此，所属技术领域的技术人员应该明了，任何属于本技术领域的技术人员在本申请揭露的技术范围内，可轻易想到的变化或替换，均落在本申请的保护范围和公开范围之内。

Claims (16)

  1. 一种芯片检测样本定量注样的系统装置，用于将定量的检测样本注入芯片的间隙腔内，所述系统装置包括：
    样本定量组件，所述样本定量组件包括本体，所述本体内设有定量腔室；
    注液腔室，所述注液腔室包括敞口的壳体，所述壳体内设有隔板，所述隔板将所述壳体分为第一腔室与第二腔室，所述第一腔室用于容纳注入的检测样本，
    其中，所述样本定量组件用于压入所述注液腔室中，以使所述本体伸入所述第一腔室内进行定量注样。
  2. 根据权利要求1所述的系统装置，其中，所述第一腔室内设置有连通所述间隙腔的至少一个进样柱，所述至少一个进样柱用于伸入所述定量腔室内；优选地，所述注液腔室还包括贯通所述进样柱的注样通道，所述注样通道用于连接所述芯片的间隙腔；优选地，所述注样通道靠近所述间隙腔的一端设有导向缺口；优选地，所述壳体靠近敞口端的内壁设有排气长槽。
  3. 根据权利要求1或2所述的系统装置，其中，所述本体的外壁设置有密封件；优选地，所述本体的外壁开设有凹槽，所述密封件设置于所述凹槽内；优选地，所述密封件为“O”型密封圈。
  4. 根据权利要求1-3中任一项所述的系统装置，其中，所述样本定量组件还包括用于固定所述本体的底座，所述底座的边缘用于在注样过程中与所述壳体的内壁过盈配合；优选地，所述底座靠近所述本体一侧的表面还设有导向件；优选地，所述本体与所述导向件之间留有空隙，所述空隙用于在注样过程中容纳所述隔板；优选地，所述底座表面开设有排气通孔；优选地，所述底座外缘设有排气缺口。
  5. 根据权利要求2-4任一项所述的系统装置，其中，所述进样柱高于所述隔板；优选地，在所述进样柱伸入所述定量腔室时，所述进样柱的外壁与所述定量腔室的内壁之间留有间隙。
  6. 根据权利要求1-5任一项所述的系统装置，其中，所述隔板靠近样本定量组件的一侧表面开设有台阶槽；优选地，所述台阶槽在沿所述本体伸入所述第一腔室的方向上包括第一凹槽与第二凹槽，所述第一凹槽的宽度大于所述第二凹槽的宽度。
  7. 一种芯片检测样本定量注样的方法，所述方法采用根据权利要求1-6中任一项所述的系统装置，所述方法包括：
    第一腔室内注入检测样本；
    将样本定量组件置于注液腔室内；
    持续下压样本定量组件，以使本体由壳体的敞口端送入第一腔室内，第一腔室内的部分检测样本溢流至第二腔室中，进入定量腔室内的检测样本流入间隙腔中实现定量注样。
  8. 根据权利要求7所述的方法，其中，所述第一腔室内设置有连通所述间隙腔的至少一个进样柱，所述进样柱包括贯通的连通至所述间隙腔的注样通道，所述定量注样包括：
    持续下压所述样本定量组件，所述进样柱逐渐伸入所述定量腔室内，将检测样本挤入进样柱与定量腔室的间隙，使检测样本通过注样通道流入所述间隙腔中；
    优选地，所述方法包括利用采集管向所述第一腔室内注入检测样本；
    优选地，所注入的检测样本位于隔板靠近样本定量组件一侧表面上的第一 凹槽与第二凹槽的对接处；
    优选地，所述方法还包括采用电机或升降机构下压所述样本定量组件。
  9. 根据权利要求7或8所述的方法，其中，所述方法还包括在定量注样过程中进行排气；优选地，采用样本定量组件的底座表面开设的排气通孔进行排气；优选地，采用样本定量组件的底座外缘设有的排气缺口进行排气；优选地，采用壳体内壁的排气长槽进行排气。
  10. 一种权利要求1-6中任一项所述的系统装置的用途，所述系统装置用于向数字微流控芯片的间隙腔内注入检测样本。
  11. 一种用于对芯片检测样本进行定量注样的系统装置，用于将定量的检测样本注入芯片的间隙腔内，所述系统装置包括注液腔室，所述注液腔室包括：
    敞口的壳体；
    设置在所述壳体内的隔板，所述隔板将所述壳体分为第一腔室与第二腔室，所述第一腔室用于容纳注入其中的检测样本以及用于容纳压入其中且与其内壁形成密封的组件，所述第二腔室用于存储从所述第一腔室溢出的样本；以及
    设置在所述第一腔室内的至少一个进样柱，所述进样柱中的注样通道用于连通所述第一腔室和所述芯片的间隙腔，其中，所述进样柱高于所述隔板。
  12. 根据权利要求11所述的系统装置，其中，所述隔板靠近壳体敞口端的一侧表面开设有台阶槽，所述台阶槽在沿朝向壳体内部的方向上包括第一凹槽与第二凹槽，所述第一凹槽的宽度大于所述第二凹槽的宽度。
  13. 根据权利要求11或12所述的系统装置，其中，所述壳体靠近敞口端的内壁设有排气长槽。
  14. 一种用于芯片检测样本定量注样的样本定量组件，包括：
    本体，所述本体内设有定量腔室，所述本体用于压入到注入有检测样本的腔室中，所述定量腔室用于容纳设置于所述腔室中的进样柱；以及
    底座，所述底座固定于所述本体。
  15. 根据权利要求14所述的样本定量组件，其中，所述本体的外壁开设有凹槽，用于安装密封件。
  16. 根据权利要求14或15所述的样本定量组件，其中，所述底座靠近所述本体一侧的表面设有导向件；优选地，所述本体与所述导向件之间留有空隙；优选地，所述底座的表面开设有排气通孔；优选地，所述底座的外缘设有排气缺口。
PCT/CN2022/108704 2021-11-17 2022-07-28 一种芯片检测样本定量注样的系统装置、方法与用途 WO2023087777A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202111359724.1A CN114100710B (zh) 2021-11-17 2021-11-17 一种芯片检测样本定量注样的系统装置、方法与用途
CN202111359724.1 2021-11-17

Publications (1)

Publication Number Publication Date
WO2023087777A1 true WO2023087777A1 (zh) 2023-05-25

Family

ID=80396193

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2022/108704 WO2023087777A1 (zh) 2021-11-17 2022-07-28 一种芯片检测样本定量注样的系统装置、方法与用途

Country Status (2)

Country Link
CN (1) CN114100710B (zh)
WO (1) WO2023087777A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116836789A (zh) * 2023-07-19 2023-10-03 徐州市检验检测中心 一种折线缓冲式pcr反应管

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114100710B (zh) * 2021-11-17 2022-12-13 江苏液滴逻辑生物技术有限公司 一种芯片检测样本定量注样的系统装置、方法与用途
CN114100712A (zh) * 2021-11-19 2022-03-01 江苏液滴逻辑生物技术有限公司 一种微流控芯片的孔注液装置、及其注液方法和用途

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060183216A1 (en) * 2005-01-21 2006-08-17 Kalyan Handique Containers for liquid storage and delivery with application to microfluidic devices
CN104391127A (zh) * 2014-11-11 2015-03-04 东莞博识生物科技有限公司 一种体外诊断测试卡加样口结构和体外诊断测试卡
CN207533259U (zh) * 2017-11-27 2018-06-26 深圳华炎微测医疗科技有限公司 Elisa检测微流控芯片和elisa检测微流控芯片体系
CN113164956A (zh) * 2018-11-29 2021-07-23 康特姆斯集团有限公司 微流体装置和方法
CN213854612U (zh) * 2020-11-09 2021-08-03 江苏奥素液芯生物技术有限公司 一种芯片的进液装置
CN213854613U (zh) * 2020-11-09 2021-08-03 江苏奥素液芯生物技术有限公司 一种芯片的进液装置
CN113578405A (zh) * 2021-08-27 2021-11-02 深圳市亚辉龙生物科技股份有限公司 微流控芯片
CN214716735U (zh) * 2021-03-31 2021-11-16 江苏液滴逻辑生物技术有限公司 一种试剂预埋与注样装置及包括其的数字微流控芯片
CN114100710A (zh) * 2021-11-17 2022-03-01 江苏液滴逻辑生物技术有限公司 一种芯片检测样本定量注样的系统装置、方法与用途

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242606A (en) * 1990-06-04 1993-09-07 Abaxis, Incorporated Sample metering port for analytical rotor having overflow chamber
CN101779129B (zh) * 2007-10-30 2013-04-03 松下电器产业株式会社 分析用仪器和使用该分析用仪器的分析装置及分析方法
CN206868242U (zh) * 2017-04-01 2018-01-12 南京岚煜生物科技有限公司 基于主动控制液体流动的微流控芯片

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060183216A1 (en) * 2005-01-21 2006-08-17 Kalyan Handique Containers for liquid storage and delivery with application to microfluidic devices
CN104391127A (zh) * 2014-11-11 2015-03-04 东莞博识生物科技有限公司 一种体外诊断测试卡加样口结构和体外诊断测试卡
CN207533259U (zh) * 2017-11-27 2018-06-26 深圳华炎微测医疗科技有限公司 Elisa检测微流控芯片和elisa检测微流控芯片体系
CN113164956A (zh) * 2018-11-29 2021-07-23 康特姆斯集团有限公司 微流体装置和方法
CN213854612U (zh) * 2020-11-09 2021-08-03 江苏奥素液芯生物技术有限公司 一种芯片的进液装置
CN213854613U (zh) * 2020-11-09 2021-08-03 江苏奥素液芯生物技术有限公司 一种芯片的进液装置
CN214716735U (zh) * 2021-03-31 2021-11-16 江苏液滴逻辑生物技术有限公司 一种试剂预埋与注样装置及包括其的数字微流控芯片
CN113578405A (zh) * 2021-08-27 2021-11-02 深圳市亚辉龙生物科技股份有限公司 微流控芯片
CN114100710A (zh) * 2021-11-17 2022-03-01 江苏液滴逻辑生物技术有限公司 一种芯片检测样本定量注样的系统装置、方法与用途

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116836789A (zh) * 2023-07-19 2023-10-03 徐州市检验检测中心 一种折线缓冲式pcr反应管

Also Published As

Publication number Publication date
CN114100710A (zh) 2022-03-01
CN114100710B (zh) 2022-12-13

Similar Documents

Publication Publication Date Title
WO2023087777A1 (zh) 一种芯片检测样本定量注样的系统装置、方法与用途
US7819029B2 (en) Automated pipette machine
US20230295700A1 (en) Microfluidic nucleic acid detection kit and detection device
CN107674819B (zh) 一种样本液滴颗粒形成装置
CN210613738U (zh) 检测芯片和检测系统
CN108795729B (zh) 一种生物芯片及其微量液体进样结构
EP3559664B1 (en) Multiple-use sensor assembly for body fluids
CN208574657U (zh) 一种带防水透气膜的移液枪
CN108241068A (zh) 一种自动化微体积移液与分配装置及其使用方法
US4900515A (en) Arrangement for determining blood cell sedimentation rate
WO2020259272A1 (zh) 采样结构、密封结构以及检测组件
CN212222935U (zh) 一种检测系统及其液滴生成装置
WO2020063795A1 (zh) 一种生物芯片及其微量液体进样结构、微量定量取样方法
CN116870977A (zh) 一种微流控联检卡壳及装置
EP3974803A1 (en) Small-sized blood viscosity measuring kit and cartridge therefor
CN208721693U (zh) 一种自动化微体积移液与分配装置
US20210252502A1 (en) Microfluidic detection unit and fluid detection method
CN219024358U (zh) 一种自动移液装置
US20160114317A1 (en) Device and system for sampling and analyzing a liquid specimen
CN218885517U (zh) 两用微量取样器
CN220657582U (zh) 一种储液单元及试剂卡盒
CN214974115U (zh) 一种移液器用连续移液装置
CN110711614A (zh) 一种设置有微流控或纳米流控结构的多通道生物反应装置
CN213376701U (zh) 一种血浆淀粉酶用加样器
CN113533263B (zh) 试剂释放装置及方法、微流控芯片装置和结核检测系统

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22894320

Country of ref document: EP

Kind code of ref document: A1