WO2023065828A1 - 多色荧光检测用多通带消偏振二向分色滤光片 - Google Patents

多色荧光检测用多通带消偏振二向分色滤光片 Download PDF

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WO2023065828A1
WO2023065828A1 PCT/CN2022/115703 CN2022115703W WO2023065828A1 WO 2023065828 A1 WO2023065828 A1 WO 2023065828A1 CN 2022115703 W CN2022115703 W CN 2022115703W WO 2023065828 A1 WO2023065828 A1 WO 2023065828A1
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depolarization
passband
fluorescence detection
film system
multicolor fluorescence
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PCT/CN2022/115703
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French (fr)
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高鹏
赵帅锋
班超
任少鹏
王瑞生
王忠连
金�秀
林晓敏
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沈阳仪表科学研究院有限公司
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B5/00—Optical elements other than lenses
    • G02B5/20—Filters
    • G02B5/28—Interference filters
    • G02B5/285—Interference filters comprising deposited thin solid films
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel

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  • the invention belongs to the field of optical filters, in particular to a multi-pass band depolarization dichroic color separation filter for multi-color fluorescence detection.
  • the dichroic mirror is one of the important optical components in the fluorescence detection instrument system, usually used at 45°, mainly used to change the direction of light and separate the spectrum.
  • the spectral characteristics of the applied dichroic mirror should match the passband position of the multi-passband filter set, which requires two
  • the film system design of the dichroic mirror has the following technical characteristics:
  • the positions of the transmission band and the reflection band are continuously adjustable to meet the application requirements of matching the position of the pass band of the multi-pass band filter group;
  • the depolarization type (long-wave pass type or short-wave pass type) of each band is flexible and adjustable to meet the application requirements of adapting to different optical path systems.
  • the present invention aims to overcome the deficiencies of the prior art and provide a spectral characteristic with high steepness and depolarization when incident at 45°, and each adaptive waveband position (transmission area and reflection area) and depolarization type (long wave) pass type or short pass type) can be flexibly adjusted for multi-color fluorescence detection with multi-passband depolarization dichroic dichroic filter.
  • the multi-pass-band depolarization dichroic filter for multi-color fluorescence detection is composed of a base and a multi-pass-band dichroic mirror film system with high-steep depolarization; the film structure is: S
  • the resolution is less than 3nm, and the wavelength range between the transmission area and the reflection area is less than 10nm.
  • the H of the present invention adopts TiO 2 , Ta 2 O 5 or Nb 2 O 5 ; the L adopts SiO 2 .
  • the high-steep, depolarized multi-passband dichroic mirror for multicolor fluorescence detection of the present invention can carry out targeted film system design according to different passband positions required for fluorescence detection.
  • the multi-pass band depolarizing dichroic mirror for multicolor fluorescence detection is composed of a substrate and a high-steep depolarization multi-pass band dichroic mirror film system; the high-steep depolarization multi-pass band dichroic mirror
  • the mirror film system has the following characteristics under the incident condition of 45°: 1.
  • the number of passbands can be adjusted arbitrarily according to the needs of fluorescence detection (generally 2 to 4), and the positions of each passband can be continuously adjusted.
  • the depolarization mode can be flexibly adjusted to meet the application requirements of detection systems with different structures.
  • Fig. 1 is the schematic diagram of film layer structure of the present invention
  • Fig. 2 is overall design curve LP of the present invention
  • Fig. 3 is the overall design curve SP of the present invention.
  • the multi-pass-band depolarization dichroic filter for multi-color fluorescence detection is composed of a base and a multi-pass-band dichroic mirror film system with high-steep depolarization; the structure of the film system is : S
  • the H in the present invention adopts TiO 2 , Ta 2 O 5 or Nb 2 O 5 ; the L adopts SiO 2 .
  • the present invention introduces the peak position coefficient a and the depolarization coefficient b, and the flexible adjustment of different peak positions can be realized by adjusting the value of a, and the depolarization design of different modes can be realized by adjusting b.
  • Nb 2 O 5 103 27 SiO 2 168 28 Nb 2 O 5 91 29 SiO 2 129 30 Nb 2 O 5 44 31 SiO 2 130 32 Nb 2 O 5 63 33 SiO 2 400 34 Nb 2 O 5 107 35 SiO 2 189 36 Nb 2 O 5 188 37 SiO 2 109 38 Nb 2 O 5 61 39 SiO 2 360 40 Nb 2 O 5 114 41 SiO 2 222 42 Nb 2 O 5 37 43 SiO 2 68 44 Nb 2 O 5 25 45 SiO 2 138 46 Nb 2 O 5 20

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Optics & Photonics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Filters (AREA)

Abstract

一种用于多色荧光检测的多色荧光检测用多通带消偏振二向分色滤光片，由基底及高陡度消偏振多通带二向分色镜膜系构成；膜系结构为：S｜［（aL HL bH LH aL） ^m］｜A；或S｜［（aL HLH bL HLH aL） ^m］｜A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料；H、L均为光学厚度为λ 0/4的膜层；a为峰位系数；b为通带消偏振系数；m为基础膜系的总体循环个数。这样的滤光片在45°入射时具有高陡度、消偏振的光谱特性且各适配波段位置和消偏振类型可以灵活调整。

Description

多色荧光检测用多通带消偏振二向分色滤光片 技术领域
本发明属光学滤光片领域，尤其涉一种用于多色荧光检测的多色荧光检测用多通带消偏振二向分色滤光片。
背景技术
二向分色镜是荧光检测仪器系统中的重要光学元件之一，通常45°使用，主要用于改变光线的方向和分离光谱。对于采用多通带滤光片组的多色荧光检测系统而言，所应用的二向分色镜在光谱特性上应与多通带滤光片组的通带位置相匹配，这就要求二向分色镜的膜系设计具备如下技术特点：
(1)透射带、反射带的位置连续可调，满足与多通带滤光片组通带位置匹配的应用需求；
(2)各使用波段过渡区的陡度高、偏振分离小，可满足在有限光谱区域内包含多个检测通道的应用需求；
(3)各波段消偏类型(长波通型或短波通型)灵活可调，满足适配不同光路系统的应用需求。
目前，多通带膜系设计方法有很多种，如：“一维晶体缺陷层模型”、“双对称结构”、“风行结构模型”、“循环嵌套模型”等，但与其配套使用的多通带二向分色镜的设计方法未见发表。
发明内容
本发明旨在克服现有技术的不足之处而提供一种在45°入射时具有高陡度、消偏振的光谱特性且各适配波段位置(透射区和反射区)和消偏振类型(长波通型或短波通型)可以灵活调整的多色荧光检测用多通带消偏振二向分色滤光片。
为解决上述技术问题，本发明是这样实现的：
多色荧光检测用多通带消偏振二向分色滤光片，由基底及高陡度消偏振的多通带二向分色镜膜系构成；所述膜系结构为：S|[(aL HL bH LH aL)^ m]|A或S|[(aL HLH bL HLH aL)^ m]|A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料；H、L均为光学厚度为λ 0/4的膜层；a为峰位系数，b为通带消偏振系数，m为基础膜系的总体循环个数。
进一步地，本发明所述高陡度消偏振的多通带二向分色镜膜系在45°入射条件下具备以下特征：各过渡带P光、S光在T＝50％Tpk处的偏振分离度小于3nm，且透射区和反射区之间的波长范围小于10nm。
进一步地，本发明所述H采用TiO 2、Ta 2O 5或Nb 2O 5；所述L采用SiO 2。
本发明多色荧光检测用高陡度、消偏振多通带二向分色镜，可以根据荧光检测需要的不同通带位置进行有针对性的膜系设计。
多色荧光检测用多通带消偏振二向分色镜由基底及高陡度消偏振的多通带二向分色镜膜系构成；其中高陡度消偏振的多通带二向分色镜膜系在45°入射条件下具备以下特征：1、通带个数可根据荧光检测需要任意可调(一般为2～4个)，且各通带位置连续可调。2、各过渡带P光、S光在T＝50％Tpk处的偏振分离度小于3nm，可以保证其透射区和反射区之间的波长范围小于10nm。3、消偏振模式可以灵活调整以适配不同结构的检测系统应用需求。
附图说明
下面结合附图和具体实施方式对本发明作进一步说明。本发明的保护范围不仅局限于下列内容的表述。
图1为本发明膜层结构示意图；
图2为本发明整体设计曲线LP；
图3为本发明整体设计曲线SP。
具体实施方式
如图所示，多色荧光检测用多通带消偏振二向分色滤光片，由基底及高陡度消偏振的多通带二向分色镜膜系构成；所述膜系结构为：S|[(aL HL bH LH aL)^ m]|A或S|[(aL HLH bL HLH aL)^ m]|A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料；H、L均为光学厚度为λ 0/4的膜层；a为峰位系数，b为通带消偏振系数，m为基础膜系的总体循环个数。
本发明所述高陡度消偏振的多通带二向分色镜膜系在45°入射条件下具备以下特征：各过渡带P光、S光在T＝50％Tpk处的偏振分离度小于3nm，且透射区和反射区之间的波长范围小于10nm。本发明所述H采用TiO 2、Ta 2O 5或Nb 2O 5；所述L采用SiO 2。
本发明引入了峰位系数a以及消偏振系数b,通过调整a的数值可以实现不同峰位的灵活调整，调整b可以实现不同模式的消偏振设计。
设计实例：
本膜系根据基本结构，按照实际需求选择高、低折射率材料以及合适的循环次数实现，如：H：Nb 2O 5；L:SiO 2；m＝7。
利用计算机软件对目标值进行优化求解，根据实际计算结果，去除极薄层，获得最终膜系。设计中各物理层厚度分布见表1：
表1各膜层物理层厚度分布
层 材料 物理厚度(nm)
1 SiO 2 53
2 Nb 2O 5 107
3 SiO 2 212
4 Nb 2O 5 137
5 SiO 2 228
6 Nb 2O 5 115
7 SiO 2 111
8 Nb 2O 5 64
9 SiO 2 350
10 Nb 2O 5 104
11 SiO 2 180
12 Nb 2O 5 108
13 SiO 2 47
14 Nb 2O 5 82
15 SiO 2 110
16 Nb 2O 5 60
17 SiO 2 355
18 Nb 2O 5 103
19 SiO 2 173
20 Nb 2O 5 89
21 SiO2 128
22 Nb 2O 5 57
23 SiO 2 115
24 Nb 2O 5 59
25 SiO 2 380
26 Nb 2O 5 103
27 SiO 2 168
28 Nb 2O 5 91
29 SiO 2 129
30 Nb 2O 5 44
31 SiO 2 130
32 Nb 2O 5 63
33 SiO 2 400
34 Nb 2O 5 107
35 SiO 2 189
36 Nb 2O 5 188
37 SiO 2 109
38 Nb 2O 5 61
39 SiO 2 360
40 Nb 2O 5 114
41 SiO 2 222
42 Nb 2O 5 37
43 SiO 2 68
44 Nb 2O 5 25
45 SiO 2 138
46 Nb 2O 5 20
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，对于本领域的技术人员来说，本实用新型可以有各种更改和变化。凡在本实用新型的精 神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本实用新型的保护范围之内。

Claims (3)

  1. 一种多色荧光检测用多通带消偏振二向分色滤光片，其特征在于，由基底及高陡度消偏振的多通带二向分色镜膜系构成；所述膜系结构为：S|[(aL HL bH LH aL)^ m]|A或S|[(aL HLH bL HLH aL)^ m]|A；其中：S为玻璃基底；A为空气；H为高折射率材料；L为低折射率材料；H、L均为光学厚度为λ 0/4的膜层；a为峰位系数，b为通带消偏振系数，m为基础膜系的总体循环个数。
  2. 根据权利要求1所述多色荧光检测用多通带消偏振二向分色滤光片，其特征在于：所述高陡度消偏振的多通带二向分色镜膜系在45°入射条件下具备以下特征：各过渡带P光、S光在T＝50％Tpk处的偏振分离度小于3nm，且透射区和反射区之间的波长范围小于10nm。
  3. 根据权利要求2所述多色荧光检测用多通带消偏振二向分色滤光片，其特征在于：所述H采用TiO 2、Ta 2O 5或Nb 2O 5；所述L采用SiO 2。
PCT/CN2022/115703 2021-10-21 2022-08-30 多色荧光检测用多通带消偏振二向分色滤光片 WO2023065828A1 (zh)

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CN115639638B (zh) * 2022-10-27 2024-04-05 沈阳仪表科学研究院有限公司 多通道荧光检测用低角度效应多陷波分色滤光片

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