WO2023065264A1 - Enrichment tube for enriching total flavonoids in chinese chives seed extraction solution - Google Patents

Enrichment tube for enriching total flavonoids in chinese chives seed extraction solution Download PDF

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Publication number
WO2023065264A1
WO2023065264A1 PCT/CN2021/125484 CN2021125484W WO2023065264A1 WO 2023065264 A1 WO2023065264 A1 WO 2023065264A1 CN 2021125484 W CN2021125484 W CN 2021125484W WO 2023065264 A1 WO2023065264 A1 WO 2023065264A1
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Prior art keywords
teflon gasket
enrichment
tube
extraction solution
total flavonoids
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PCT/CN2021/125484
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French (fr)
Chinese (zh)
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谢秋敏
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南通优元美术图案设计有限公司
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Priority to DE212021000175.0U priority Critical patent/DE212021000175U1/en
Priority to PCT/CN2021/125484 priority patent/WO2023065264A1/en
Publication of WO2023065264A1 publication Critical patent/WO2023065264A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/265—Adsorption chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0288—Applications, solvents

Definitions

  • the utility model specifically relates to an enrichment tube for enriching total flavonoids in leek seed extract.
  • Leek seeds contain various active ingredients such as sulfides, flavonoids, and vitamin C. They have the functions of warming and nourishing the liver and kidneys, and are one of the commonly used medicinal materials in Chinese medicine in my country. Studies have shown that the flavonoids in chive seeds have several benefits.
  • the purpose of the utility model is to provide an enrichment tube for enriching total flavonoids in leek seed extract.
  • An enrichment tube for enriching total flavonoids in leek seed extract including a tube body, a Teflon gasket and a resin layer; wherein, the top of the tube body is provided with an inlet, and the bottom is provided with an outlet, and the tube body is arranged from bottom to bottom. And the first Teflon gasket, the second Teflon gasket and the third Teflon gasket are arranged in turn, and the LSA-30 gasket is between the first Teflon gasket and the second Teflon gasket.
  • the resin layer there is a D-101 resin layer between the second Teflon gasket and the third Teflon gasket, and an extraction solution holding chamber is between the third Teflon gasket and the inlet.
  • the tube body is made of polytetrafluoroethylene.
  • a pressurizing piston adapted to the inner diameter of the tube is also included.
  • the enrichment tube provided by the utility model can effectively enrich the total flavonoids in the leek seed extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.
  • Fig. 1 is the schematic diagram of the enrichment tube of the total flavonoids in the enrichment leek seed extract; Wherein, 1 is the tube body, 2 is the inlet, 3 is the outlet, 4 is the first Teflon gasket, 5 is the second Teflon Dragon gasket, 6 is the third Teflon gasket, 7 is the LSA-30 resin layer, 8 is the D-101 resin layer, and 9 is the extraction solution holding chamber.
  • a kind of enrichment tube for enriching total flavonoids in leek seed extract as shown in Figure 1 comprises tube body 1, Teflon gasket and resin layer; Wherein, the top of described tube body 1 is provided with inlet 2, There is an outlet 3 at the bottom, and the first Teflon gasket 4, the second Teflon gasket 5 and the third Teflon gasket 6 are arranged in the tube body from bottom to top, and the first Teflon gasket 4 Between the second Teflon gasket 5 is an LSA-30 resin layer 7, between the second Teflon gasket 5 and the third Teflon gasket 6 is a D-101 resin layer 8, the third Teflon Between the dragon gasket 6 and the inlet 2 is an extraction liquid containing chamber 9 .
  • the pipe body 1 is made of polytetrafluoroethylene.
  • a pressurizing piston adapted to the inner diameter of the tube can also be provided to speed up the descending speed of the extraction liquid in the extraction liquid containing chamber 9 .
  • the enrichment tube provided by the utility model can effectively enrich the total flavonoids in the leek seed extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.

Abstract

Provided in the present invention is an enrichment tube for enriching the total flavonoids in a Chinese chives seed extraction solution. The enrichment tube comprises a tube body, a Teflon gasket and a resin layer, wherein the tube body is provided with an inlet at the top thereof and an outlet at the bottom thereof, and the tube body is sequentially provided with a first Teflon gasket, a second Teflon gasket and a third Teflon gasket from bottom to top. An LSA-30 resin layer is provided between the first Teflon gasket and the second Teflon gasket, a D-101 resin layer is provided between the second Teflon gasket and the third Teflon gasket, and an extraction solution containing cavity is provided between the third Teflon gasket and the inlet. The enrichment tube can effectively enrich the total flavonoids in a Chinese chives seed extraction solution, and the content of the total flavonoids in the extraction solution after enrichment is nearly 10 times that in the extraction solution before enrichment.

Description

一种富集韭菜籽提取液中总黄酮的富集管An enrichment tube for enriching total flavonoids in leek seed extract 技术领域technical field
本实用新型具体涉及一种富集韭菜籽提取液中总黄酮的富集管。The utility model specifically relates to an enrichment tube for enriching total flavonoids in leek seed extract.
背景技术Background technique
韭菜籽含有硫化物、黄酮、维生素C等多种活性成分，具有温补肝肾等功效，是我国中医药学中的常用药材之一。研究表明，韭菜籽中的黄酮类化合物具有多种功效。Leek seeds contain various active ingredients such as sulfides, flavonoids, and vitamin C. They have the functions of warming and nourishing the liver and kidneys, and are one of the commonly used medicinal materials in Chinese medicine in my country. Studies have shown that the flavonoids in chive seeds have several benefits.
因此，富集韭菜籽中的总黄酮具有重要意义。Therefore, it is of great significance to enrich the total flavonoids in leek seeds.
发明内容Contents of the invention
本实用新型的目的在于提供一种富集韭菜籽提取液中总黄酮的富集管。The purpose of the utility model is to provide an enrichment tube for enriching total flavonoids in leek seed extract.
本实用新型通过如下技术方案得以实现：The utility model is realized through the following technical solutions:
一种富集韭菜籽提取液中总黄酮的富集管，包括管体、特氟龙垫片和树脂层；其中，所述管体的顶部设有入口，底部设有出口，管体内自下而上依次设有第一特氟龙垫片、第二特氟龙垫片和第三特氟龙垫片，第一特氟龙垫片和第二特氟龙垫片之间为LSA-30树脂层，第二特氟龙垫片和第三特氟龙垫片之间为D-101树脂层，第三特氟龙垫片与入口之间为提取液容纳腔。An enrichment tube for enriching total flavonoids in leek seed extract, including a tube body, a Teflon gasket and a resin layer; wherein, the top of the tube body is provided with an inlet, and the bottom is provided with an outlet, and the tube body is arranged from bottom to bottom. And the first Teflon gasket, the second Teflon gasket and the third Teflon gasket are arranged in turn, and the LSA-30 gasket is between the first Teflon gasket and the second Teflon gasket. For the resin layer, there is a D-101 resin layer between the second Teflon gasket and the third Teflon gasket, and an extraction solution holding chamber is between the third Teflon gasket and the inlet.
优选地，所述管体为聚四氟乙烯材质。Preferably, the tube body is made of polytetrafluoroethylene.
优选地，还包括与管体内径适配的加压活塞。Preferably, a pressurizing piston adapted to the inner diameter of the tube is also included.
本实用新型的优点：Advantage of the utility model:
本实用新型提供的富集管可以有效富集韭菜籽提取液中总黄酮，富集后提取液中总黄酮含量是富集前提取液中总黄酮含量的近10倍。The enrichment tube provided by the utility model can effectively enrich the total flavonoids in the leek seed extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.
附图说明Description of drawings
图1为富集韭菜籽提取液中总黄酮的富集管的示意图；其中，1为管体，2为入口，3为出口，4为第一特氟龙垫片，5为第二特氟龙垫片，6为第三特氟龙垫片，7为LSA-30树脂层，8为D-101树脂层，9为提取液容纳腔。Fig. 1 is the schematic diagram of the enrichment tube of the total flavonoids in the enrichment leek seed extract; Wherein, 1 is the tube body, 2 is the inlet, 3 is the outlet, 4 is the first Teflon gasket, 5 is the second Teflon Dragon gasket, 6 is the third Teflon gasket, 7 is the LSA-30 resin layer, 8 is the D-101 resin layer, and 9 is the extraction solution holding chamber.
具体实施方式Detailed ways
下面结合附图和实施例具体介绍本实用新型实质性内容。The substantive content of the utility model is specifically introduced below in conjunction with the accompanying drawings and embodiments.
如图1所示的一种富集韭菜籽提取液中总黄酮的富集管，包括管体1、特氟龙垫片和树脂层；其中，所述管体1的顶部设有入口2，底部设有出口3，管体内自下而上依次设有第一特氟龙垫片4、第二特氟龙垫片5和第三特氟龙垫片6，第一特氟龙垫片4和第二特氟龙垫片5之间为LSA-30树脂层7，第二特氟龙垫片5和第三特氟龙垫片6之间为D-101树脂层8， 第三特氟龙垫片6与入口2之间为提取液容纳腔9。管体1为聚四氟乙烯材质。还可以设置与管体内径适配的加压活塞，加快提取液容纳腔9中提取液的下行速度。A kind of enrichment tube for enriching total flavonoids in leek seed extract as shown in Figure 1, comprises tube body 1, Teflon gasket and resin layer; Wherein, the top of described tube body 1 is provided with inlet 2, There is an outlet 3 at the bottom, and the first Teflon gasket 4, the second Teflon gasket 5 and the third Teflon gasket 6 are arranged in the tube body from bottom to top, and the first Teflon gasket 4 Between the second Teflon gasket 5 is an LSA-30 resin layer 7, between the second Teflon gasket 5 and the third Teflon gasket 6 is a D-101 resin layer 8, the third Teflon Between the dragon gasket 6 and the inlet 2 is an extraction liquid containing chamber 9 . The pipe body 1 is made of polytetrafluoroethylene. A pressurizing piston adapted to the inner diameter of the tube can also be provided to speed up the descending speed of the extraction liquid in the extraction liquid containing chamber 9 .
应用示例：Application example:
取适量干燥的韭菜籽，粉碎，用75％乙醇回流提取3次，每次2h，滤过，合并乙醇液，减压浓缩得韭菜籽总提取物。取适量韭菜籽总提取物用去离子水溶解配制成5mg/mL的韭菜籽提取液。取适量韭菜籽提取液加满图1所示的富集管的提取液容纳腔9，待富集管中的液体全部流干后，用50％乙醇加满提取液容纳腔9，收集50％乙醇洗脱液。分别测定韭菜籽提取液和50％乙醇洗脱液中总黄酮的浓度，50％乙醇洗脱液中总黄酮浓度是韭菜籽提取液中总黄酮浓度的9.25倍。Take an appropriate amount of dry chive seeds, crush them, reflux extract with 75% ethanol for 3 times, each time for 2 hours, filter, combine the ethanol solution, and concentrate under reduced pressure to obtain the total chive seed extract. Take an appropriate amount of total leek seed extract and dissolve it in deionized water to prepare a 5 mg/mL leek seed extract. Take an appropriate amount of leek seed extract to fill up the extracting solution chamber 9 of the enrichment tube shown in Figure 1. After the liquid in the enrichment tube is completely drained, fill up the extracting solution containing chamber 9 with 50% ethanol, and collect 50% ethanol eluent. The concentrations of total flavonoids in the leek seed extract and 50% ethanol eluate were determined respectively, and the total flavonoid concentration in the 50% ethanol eluate was 9.25 times that of the leek seed extract.
本实用新型提供的富集管可以有效富集韭菜籽提取液中总黄酮，富集后提取液中总黄酮含量是富集前提取液中总黄酮含量的近10倍。The enrichment tube provided by the utility model can effectively enrich the total flavonoids in the leek seed extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.
上述实施例的作用在于具体介绍本实用新型的实质性内容，但本领域技术人员应当知道，不应将本实用新型的保护范围局限于该具体实施例。The purpose of the above embodiments is to specifically introduce the substantive content of the utility model, but those skilled in the art should know that the protection scope of the utility model should not be limited to the specific examples.

Claims (3)

  1. 一种富集韭菜籽提取液中总黄酮的富集管，其特征在于：包括管体、特氟龙垫片和树脂层；其中，所述管体的顶部设有入口，底部设有出口，管体内自下而上依次设有第一特氟龙垫片、第二特氟龙垫片和第三特氟龙垫片，第一特氟龙垫片和第二特氟龙垫片之间为LSA-30树脂层，第二特氟龙垫片和第三特氟龙垫片之间为D-101树脂层，第三特氟龙垫片与入口之间为提取液容纳腔。An enrichment tube for enriching total flavonoids in the leek seed extract is characterized in that it includes a tube body, a Teflon gasket and a resin layer; wherein the top of the tube body is provided with an inlet, and the bottom is provided with an outlet, The first Teflon gasket, the second Teflon gasket and the third Teflon gasket are arranged in sequence from bottom to top in the tube body, between the first Teflon gasket and the second Teflon gasket It is an LSA-30 resin layer, between the second Teflon gasket and the third Teflon gasket is a D-101 resin layer, and between the third Teflon gasket and the inlet is an extraction solution chamber.
  2. 根据权利要求1所述的富集管，其特征在于：所述管体为聚四氟乙烯材质。The enrichment tube according to claim 1, wherein the tube body is made of polytetrafluoroethylene.
  3. 根据权利要求1所述的富集管，其特征在于：还包括与管体内径适配的加压活塞。The enrichment tube according to claim 1, further comprising a pressurizing piston adapted to the inner diameter of the tube.
PCT/CN2021/125484 2021-10-22 2021-10-22 Enrichment tube for enriching total flavonoids in chinese chives seed extraction solution WO2023065264A1 (en)

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DE212021000175.0U DE212021000175U1 (en) 2021-10-22 2021-10-22 Enrichment tube for enriching total flavonoids in leek seed extract
PCT/CN2021/125484 WO2023065264A1 (en) 2021-10-22 2021-10-22 Enrichment tube for enriching total flavonoids in chinese chives seed extraction solution

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1806880A (en) * 2005-12-19 2006-07-26 东南大学 Solid phase micro-extraction device based on nanometer fiber
CN1938585A (en) * 2004-03-31 2007-03-28 财团法人杂贺技术研究所 Stationary phase extraction cartridge
CN102078705A (en) * 2010-12-28 2011-06-01 苏州先伟纳米技术有限公司 Tandem nano fiber solid phase extraction column
CN102974129A (en) * 2012-12-10 2013-03-20 浙江省海洋水产研究所 Multi-stage combined type multi-wall carbon nano tube solid phase extraction column
CN103585424A (en) * 2013-11-21 2014-02-19 华东理工大学 Method for extracting total flavone from Chinese chive seeds
CN105445061A (en) * 2016-02-01 2016-03-30 南京工业职业技术学院 Active sampling system of persistent organic pollutants PAHs in atmosphere

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1938585A (en) * 2004-03-31 2007-03-28 财团法人杂贺技术研究所 Stationary phase extraction cartridge
CN1806880A (en) * 2005-12-19 2006-07-26 东南大学 Solid phase micro-extraction device based on nanometer fiber
CN102078705A (en) * 2010-12-28 2011-06-01 苏州先伟纳米技术有限公司 Tandem nano fiber solid phase extraction column
CN102974129A (en) * 2012-12-10 2013-03-20 浙江省海洋水产研究所 Multi-stage combined type multi-wall carbon nano tube solid phase extraction column
CN103585424A (en) * 2013-11-21 2014-02-19 华东理工大学 Method for extracting total flavone from Chinese chive seeds
CN105445061A (en) * 2016-02-01 2016-03-30 南京工业职业技术学院 Active sampling system of persistent organic pollutants PAHs in atmosphere

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