WO2023060621A1 - Enrichment tube for enriching total flavones in nageia nagi kuntze leaf extracting solution - Google Patents

Enrichment tube for enriching total flavones in nageia nagi kuntze leaf extracting solution Download PDF

Info

Publication number
WO2023060621A1
WO2023060621A1 PCT/CN2021/124278 CN2021124278W WO2023060621A1 WO 2023060621 A1 WO2023060621 A1 WO 2023060621A1 CN 2021124278 W CN2021124278 W CN 2021124278W WO 2023060621 A1 WO2023060621 A1 WO 2023060621A1
Authority
WO
WIPO (PCT)
Prior art keywords
teflon gasket
tube
enrichment
extracting solution
teflon
Prior art date
Application number
PCT/CN2021/124278
Other languages
French (fr)
Chinese (zh)
Inventor
谭玲
Original Assignee
南通市台盈新材料科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE212021000151.3U priority Critical patent/DE212021000151U1/en
Application filed by 南通市台盈新材料科技有限公司 filed Critical 南通市台盈新材料科技有限公司
Priority to PCT/CN2021/124278 priority patent/WO2023060621A1/en
Publication of WO2023060621A1 publication Critical patent/WO2023060621A1/en

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/265—Adsorption chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0288—Applications, solvents

Definitions

  • the utility model specifically relates to an enrichment tube for enriching total flavonoids in bamboo and cypress leaf extracts.
  • Nageia nagi (Thunberg) Kuntze is a plant of the genus Nageia nagi in the Podocarpus family, and the leaves of Thunberg have antitumor and cardiovascular activity. Flavonoids are important chemical constituents in bamboo cypress leaves, which have physiological functions such as anti-inflammatory, anti-oxidation and antibacterial.
  • the purpose of the utility model is to provide an enrichment tube for enriching total flavonoids in bamboo and cypress leaf extract.
  • An enrichment tube for enriching total flavonoids in the bamboo and cypress leaf extract including a tube body, a Teflon gasket and a resin layer; wherein, the top of the tube body is provided with an inlet, and the bottom is provided with an outlet, and the tube body is from bottom to bottom.
  • the first Teflon gasket, the second Teflon gasket and the third Teflon gasket are arranged in turn on the top, and the XDA-6 spacer is between the first Teflon gasket and the second Teflon gasket.
  • the resin layer, between the second Teflon gasket and the third Teflon gasket is the AB-8 resin layer, and between the third Teflon gasket and the inlet is the extraction solution containing chamber.
  • the tube body is made of polytetrafluoroethylene.
  • a pressurizing piston adapted to the inner diameter of the tube is also included.
  • the enrichment tube provided by the utility model can effectively enrich the total flavonoids in the bamboo cypress leaf extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.
  • Fig. 1 is the schematic diagram of the enrichment tube of the total flavonoids in the enrichment bamboo and cypress leaf extract; Wherein, 1 is the tube body, 2 is the inlet, 3 is the outlet, 4 is the first Teflon gasket, 5 is the second Teflon Dragon gasket, 6 is the third Teflon gasket, 7 is the XDA-6 resin layer, 8 is the AB-8 resin layer, and 9 is the extraction solution holding chamber.
  • a kind of enrichment tube for enriching total flavonoids in bamboo and cypress leaf extract as shown in Figure 1 comprises tube body 1, Teflon gasket and resin layer; Wherein, the top of described tube body 1 is provided with inlet 2, There is an outlet 3 at the bottom, and the first Teflon gasket 4, the second Teflon gasket 5 and the third Teflon gasket 6 are arranged in the tube body from bottom to top, and the first Teflon gasket 4 Between the second Teflon gasket 5 is an XDA-6 resin layer 7, between the second Teflon gasket 5 and the third Teflon gasket 6 is an AB-8 resin layer 8, and the third Teflon gasket Between the dragon gasket 6 and the inlet 2 is an extraction liquid containing chamber 9 .
  • the pipe body 1 is made of polytetrafluoroethylene.
  • a pressurizing piston adapted to the inner diameter of the tube can also be provided to speed up the descending speed of the extraction liquid in the extraction liquid containing chamber 9 .
  • the enrichment tube provided by the utility model can effectively enrich the total flavonoids in the bamboo cypress leaf extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.

Abstract

The present invention provides an enrichment tube for enriching total flavones in a nageia nagi kuntze leaf extracting solution, the tube comprising a tube body, Teflon gaskets, and a resin layer, wherein an inlet is formed in the top of the tube body; an outlet is formed in the bottom of the tube body; a first Teflon gasket, a second Teflon gasket, and a third Teflon gasket are sequentially arranged in the tube body from bottom to top; an XDA-6 resin layer is arranged between the first Teflon gasket and the second Teflon gasket; an AB-8 resin layer is arranged between the second Teflon gasket and the third Teflon gasket; and an extracting solution accommodating cavity is arranged between the third Teflon gasket and the inlet. The enrichment tube can effectively enrich the total flavones in the nageia nagi kuntze leaf extracting solution, and the content of the total flavones in the extracting solution after enrichment is nearly 10 times the content of the total flavones in the extracting solution before enrichment.

Description

一种富集竹柏叶提取液中总黄酮的富集管An enrichment tube for enriching total flavonoids in bamboo and cypress leaf extract 技术领域technical field
本实用新型具体涉及一种富集竹柏叶提取液中总黄酮的富集管。The utility model specifically relates to an enrichment tube for enriching total flavonoids in bamboo and cypress leaf extracts.
背景技术Background technique
竹柏Nageia nagi(Thunberg)Kuntze是罗汉松科竹柏属植物，竹柏叶具有抗肿瘤和治疗心血管疾病的活性。黄酮是竹柏叶中重要的化学成分，具有抗炎、抗氧化和抑菌等生理功能。Nageia nagi (Thunberg) Kuntze is a plant of the genus Nageia nagi in the Podocarpus family, and the leaves of Thunberg have antitumor and cardiovascular activity. Flavonoids are important chemical constituents in bamboo cypress leaves, which have physiological functions such as anti-inflammatory, anti-oxidation and antibacterial.
因此，富集竹柏叶中的总黄酮具有重要意义。Therefore, it is of great significance to enrich the total flavonoids in bamboo leaves.
发明内容Contents of the invention
本实用新型的目的在于提供一种富集竹柏叶提取液中总黄酮的富集管。The purpose of the utility model is to provide an enrichment tube for enriching total flavonoids in bamboo and cypress leaf extract.
本实用新型通过如下技术方案得以实现：The utility model is realized through the following technical solutions:
一种富集竹柏叶提取液中总黄酮的富集管，包括管体、特氟龙垫片和树脂层；其中，所述管体的顶部设有入口，底部设有出口，管体内自下而上依次设有第一特氟龙垫片、第二特氟龙垫片和第三特氟龙垫片，第一特氟龙垫片和第二特氟龙垫片之间为XDA-6树脂层，第二特氟龙垫片和第三特氟龙垫片之间为AB-8树脂层，第三特氟龙垫片与入口之间为提取液容纳腔。An enrichment tube for enriching total flavonoids in the bamboo and cypress leaf extract, including a tube body, a Teflon gasket and a resin layer; wherein, the top of the tube body is provided with an inlet, and the bottom is provided with an outlet, and the tube body is from bottom to bottom. The first Teflon gasket, the second Teflon gasket and the third Teflon gasket are arranged in turn on the top, and the XDA-6 spacer is between the first Teflon gasket and the second Teflon gasket. The resin layer, between the second Teflon gasket and the third Teflon gasket is the AB-8 resin layer, and between the third Teflon gasket and the inlet is the extraction solution containing chamber.
优选地，所述管体为聚四氟乙烯材质。Preferably, the tube body is made of polytetrafluoroethylene.
优选地，还包括与管体内径适配的加压活塞。Preferably, a pressurizing piston adapted to the inner diameter of the tube is also included.
本实用新型的优点：Advantage of the utility model:
本实用新型提供的富集管可以有效富集竹柏叶提取液中总黄酮，富集后提取液中总黄酮含量是富集前提取液中总黄酮含量的近10倍。The enrichment tube provided by the utility model can effectively enrich the total flavonoids in the bamboo cypress leaf extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.
附图说明Description of drawings
图1为富集竹柏叶提取液中总黄酮的富集管的示意图；其中，1为管体，2为入口，3为出口，4为第一特氟龙垫片，5为第二特氟龙垫片，6为第三特氟龙垫片，7为XDA-6树脂层，8为AB-8树脂层，9为提取液容纳腔。Fig. 1 is the schematic diagram of the enrichment tube of the total flavonoids in the enrichment bamboo and cypress leaf extract; Wherein, 1 is the tube body, 2 is the inlet, 3 is the outlet, 4 is the first Teflon gasket, 5 is the second Teflon Dragon gasket, 6 is the third Teflon gasket, 7 is the XDA-6 resin layer, 8 is the AB-8 resin layer, and 9 is the extraction solution holding chamber.
具体实施方式Detailed ways
下面结合附图和实施例具体介绍本实用新型实质性内容。The substantive content of the utility model is specifically introduced below in conjunction with the accompanying drawings and embodiments.
如图1所示的一种富集竹柏叶提取液中总黄酮的富集管，包括管体1、特氟龙垫片和树脂层；其中，所述管体1的顶部设有入口2，底部设有出口3，管体内自下而上依次设有第一特氟龙垫片4、第二特氟龙垫片5和第三特氟龙垫片6，第一特氟龙垫片4和第二特氟龙垫片5之间为XDA-6树脂层7，第二特氟龙垫片5和第三特氟龙垫片6之间为AB-8树脂层8，第 三特氟龙垫片6与入口2之间为提取液容纳腔9。管体1为聚四氟乙烯材质。还可以设置与管体内径适配的加压活塞，加快提取液容纳腔9中提取液的下行速度。A kind of enrichment tube for enriching total flavonoids in bamboo and cypress leaf extract as shown in Figure 1, comprises tube body 1, Teflon gasket and resin layer; Wherein, the top of described tube body 1 is provided with inlet 2, There is an outlet 3 at the bottom, and the first Teflon gasket 4, the second Teflon gasket 5 and the third Teflon gasket 6 are arranged in the tube body from bottom to top, and the first Teflon gasket 4 Between the second Teflon gasket 5 is an XDA-6 resin layer 7, between the second Teflon gasket 5 and the third Teflon gasket 6 is an AB-8 resin layer 8, and the third Teflon gasket Between the dragon gasket 6 and the inlet 2 is an extraction liquid containing chamber 9 . The pipe body 1 is made of polytetrafluoroethylene. A pressurizing piston adapted to the inner diameter of the tube can also be provided to speed up the descending speed of the extraction liquid in the extraction liquid containing chamber 9 .
应用示例：Application example:
取适量干燥的竹柏叶，用60％乙醇回流提取3次，每次2h，滤过，合并乙醇液，减压浓缩得竹柏叶总提取物。取适量竹柏叶总提取物用去离子水溶解配制成5mg/mL的竹柏叶提取液。取适量竹柏叶提取液加满图1所示的富集管的提取液容纳腔9，待富集管中的液体全部流干后，用50％乙醇加满提取液容纳腔9，收集50％乙醇洗脱液。分别测定竹柏叶提取液和50％乙醇洗脱液中总黄酮的浓度，50％乙醇洗脱液中总黄酮浓度是竹柏叶提取液中总黄酮浓度的9.37倍。Take an appropriate amount of dried bamboo and cypress leaves, reflux extraction with 60% ethanol for 3 times, each time for 2 hours, filter, combine the ethanol solution, and concentrate under reduced pressure to obtain the total extract of bamboo and cypress leaves. Take an appropriate amount of the total extract of bamboo and cypress leaves and dissolve it in deionized water to prepare a 5 mg/mL extract of bamboo and cypress leaves. Take an appropriate amount of bamboo and cypress leaf extract to fill up the extract solution chamber 9 of the enrichment tube shown in Figure 1, after the liquid in the enrichment tube is completely drained, fill up the extract solution chamber 9 with 50% ethanol, and collect 50% ethanol eluent. The concentrations of total flavonoids in bamboo cypress leaf extract and 50% ethanol eluate were determined respectively, and the total flavonoid concentration in 50% ethanol eluate was 9.37 times that of bamboo cypress leaf extract.
本实用新型提供的富集管可以有效富集竹柏叶提取液中总黄酮，富集后提取液中总黄酮含量是富集前提取液中总黄酮含量的近10倍。The enrichment tube provided by the utility model can effectively enrich the total flavonoids in the bamboo cypress leaf extract, and the content of the total flavonoids in the extract after enrichment is nearly 10 times that in the extract before enrichment.
上述实施例的作用在于具体介绍本实用新型的实质性内容，但本领域技术人员应当知道，不应将本实用新型的保护范围局限于该具体实施例。The purpose of the above embodiments is to specifically introduce the substantive content of the utility model, but those skilled in the art should know that the protection scope of the utility model should not be limited to the specific examples.

Claims (3)

  1. 一种富集竹柏叶提取液中总黄酮的富集管，其特征在于：包括管体、特氟龙垫片和树脂层；其中，所述管体的顶部设有入口，底部设有出口，管体内自下而上依次设有第一特氟龙垫片、第二特氟龙垫片和第三特氟龙垫片，第一特氟龙垫片和第二特氟龙垫片之间为XDA-6树脂层，第二特氟龙垫片和第三特氟龙垫片之间为AB-8树脂层，第三特氟龙垫片与入口之间为提取液容纳腔。An enrichment tube for enriching total flavonoids in bamboo and cypress leaf extract is characterized in that: it includes a tube body, a Teflon gasket and a resin layer; wherein, the top of the tube body is provided with an inlet, and the bottom is provided with an outlet, The first Teflon gasket, the second Teflon gasket and the third Teflon gasket are arranged in sequence from bottom to top in the tube body, between the first Teflon gasket and the second Teflon gasket It is an XDA-6 resin layer, between the second Teflon gasket and the third Teflon gasket is an AB-8 resin layer, and between the third Teflon gasket and the inlet is an extraction solution containing chamber.
  2. 根据权利要求1所述的富集管，其特征在于：所述管体为聚四氟乙烯材质。The enrichment tube according to claim 1, wherein the tube body is made of polytetrafluoroethylene.
  3. 根据权利要求1所述的富集管，其特征在于：还包括与管体内径适配的加压活塞。The enrichment tube according to claim 1, further comprising a pressurizing piston adapted to the inner diameter of the tube.
PCT/CN2021/124278 2021-10-17 2021-10-17 Enrichment tube for enriching total flavones in nageia nagi kuntze leaf extracting solution WO2023060621A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE212021000151.3U DE212021000151U1 (en) 2021-10-17 2021-10-16 Enrichment tubes for enriching the total flavonoids in bamboo and cypress leaf extract
PCT/CN2021/124278 WO2023060621A1 (en) 2021-10-17 2021-10-17 Enrichment tube for enriching total flavones in nageia nagi kuntze leaf extracting solution

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2021/124278 WO2023060621A1 (en) 2021-10-17 2021-10-17 Enrichment tube for enriching total flavones in nageia nagi kuntze leaf extracting solution

Publications (1)

Publication Number Publication Date
WO2023060621A1 true WO2023060621A1 (en) 2023-04-20

Family

ID=79689600

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2021/124278 WO2023060621A1 (en) 2021-10-17 2021-10-17 Enrichment tube for enriching total flavones in nageia nagi kuntze leaf extracting solution

Country Status (2)

Country Link
DE (1) DE212021000151U1 (en)
WO (1) WO2023060621A1 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1806880A (en) * 2005-12-19 2006-07-26 东南大学 Solid phase micro-extraction device based on nanometer fiber
CN1938585A (en) * 2004-03-31 2007-03-28 财团法人杂贺技术研究所 Stationary phase extraction cartridge
CN101057905A (en) * 2007-05-11 2007-10-24 桂林工学院 Microwave extraction method for cordate houttuynia total flavones
CN102078705A (en) * 2010-12-28 2011-06-01 苏州先伟纳米技术有限公司 Tandem nano fiber solid phase extraction column
CN102974129A (en) * 2012-12-10 2013-03-20 浙江省海洋水产研究所 Multi-stage combined type multi-wall carbon nano tube solid phase extraction column
CN105445061A (en) * 2016-02-01 2016-03-30 南京工业职业技术学院 Active sampling system of persistent organic pollutants PAHs in atmosphere

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1938585A (en) * 2004-03-31 2007-03-28 财团法人杂贺技术研究所 Stationary phase extraction cartridge
CN1806880A (en) * 2005-12-19 2006-07-26 东南大学 Solid phase micro-extraction device based on nanometer fiber
CN101057905A (en) * 2007-05-11 2007-10-24 桂林工学院 Microwave extraction method for cordate houttuynia total flavones
CN102078705A (en) * 2010-12-28 2011-06-01 苏州先伟纳米技术有限公司 Tandem nano fiber solid phase extraction column
CN102974129A (en) * 2012-12-10 2013-03-20 浙江省海洋水产研究所 Multi-stage combined type multi-wall carbon nano tube solid phase extraction column
CN105445061A (en) * 2016-02-01 2016-03-30 南京工业职业技术学院 Active sampling system of persistent organic pollutants PAHs in atmosphere

Also Published As

Publication number Publication date
DE212021000151U1 (en) 2022-01-03
DE212021000151U8 (en) 2023-06-29

Similar Documents

Publication Publication Date Title
CN107652260B (en) Method for preparing natural flavanone compound by high-speed countercurrent chromatography rapid separation
CN107151203A (en) Separate the method for preparing natural naphthoquinone compound
AU2020101569A4 (en) A Method of Extracting Xanthotoxol from the Root of Angelica Dahurica
CN111700902B (en) Hawthorn procyanidine-jujube polysaccharide composition and preparation method thereof
CN107793456A (en) Anthocyanidin of extraction purification and preparation method thereof from perilla leaf
CN101348474A (en) Method for preparing salvianolic acid B and tanshinol from Salvia miltiorrhiza stem
WO2023060621A1 (en) Enrichment tube for enriching total flavones in nageia nagi kuntze leaf extracting solution
CN103224491A (en) Method for extracting high-purity puerarin by using water as solvent
CN1709900A (en) Comprehensive extracting mehtod for balsam pear saponin and balsam pear polysaccharide
CN105085534B (en) A kind of alkaloid compound and its extraction separation method
CN101210041A (en) Method for separating and preparing tanshinone IIA chemical reference substance
CN107213180B (en) Separation and extraction method of notoginseng flavone
WO2023060622A1 (en) Enrichment tube for enriching total flavones in epimedium brevicomu maxim leaf extracting solution
WO2023065265A1 (en) Enrichment tube for enriching total flavonoids in houttuynia cordata extraction solution
CN108101923B (en) Separation and purification method of glabridin monomer
WO2023060623A1 (en) Enrichment tube for enriching total alkaloids in datura metel l. leaf extracting solution
WO2023060624A1 (en) Enrichment tube for enriching total flavones in datura metel l. leaf extracting solution
WO2023065264A1 (en) Enrichment tube for enriching total flavonoids in chinese chives seed extraction solution
CN111875483B (en) Method for extracting lignanoid compounds from schisandra chinensis
CN109627153B (en) Method for extracting and separating p-hydroxybenzaldehyde from nostoc commune
CN107913297A (en) A kind of strophanthus divaricatus flavone c-glycoside composition and its preparation method and application
CN108114024A (en) A kind of extracting method of Guava Leaf terpene substances
CN104447720B (en) Method for separating vicenin-2 from linearstripe rabdosia herb
CN103044253A (en) Extraction separation method of rosmarinic acid in salvia castabea diels f. tomentosa stib
CN113512021A (en) Method for simultaneously extracting procyanidine and polysaccharide from rosa davurica pall seeds

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21960350

Country of ref document: EP

Kind code of ref document: A1