WO2023059047A1 - Composition for preventing or treating hair loss, comprising samdachal-jo (jeju glutinous millet) extract - Google Patents

Abstract

The present invention relates to a composition for preventing, treating, or alleviating hair loss, comprising a Samdachal-jo (Jeju glutinous millet) extract. Specifically, the present invention relates to a pharmaceutical composition or a food composition for preventing, treating, or alleviating hair loss by increasing the proliferation of dermal papilla cells, the composition comprising a Samdachal-jo extract as an active ingredient.

Description

Composition for the prevention or treatment of hair loss containing an extract of cedar algae

The present invention relates to a composition for preventing, treating, or improving hair loss comprising an extract of cedar algae. Specifically, the present invention relates to a pharmaceutical composition or a food composition for preventing, treating, or improving hair loss by increasing the proliferation of dermal papilla cells, comprising an extract of Samdacha algae as an active ingredient.

Dermal papilla cells are key cells responsible for the development and growth of hair, and are located at the bottom of the hair root, which is the root of the hair subcutaneously. In fact, when dermal papilla cells are collected from the scalp, cultured, and transplanted, new hair grows. However, there are several difficulties in using dermal papilla cells as a hair loss treatment. First, it is difficult to separate from the scalp, the culture conditions are difficult, and it is difficult to culture a sufficient amount of cells. In addition, when cultured a lot, there is a limit in that the hair regeneration ability is significantly lowered.

Since human hair has a certain hair growth cycle, a certain number of hairs is always maintained. However, as hair loss progresses, the dermal papilla present in the hair root becomes smaller, and when the dermal papilla becomes smaller, the thickness of the hair becomes thinner, and at the same time, the hair growth cycle is shortened. Therefore, as hair loss progresses, the hair becomes very thin and the hair growth cycle becomes shorter, so it grows a little and then falls out.

It is known that the cause of hair loss is a combination of factors such as genetic causes and male hormones, as well as severe physical and mental stress such as endocrine diseases, nutritional deficiencies, drug use, childbirth, fever, and surgery. In recent years, not only male pattern baldness, but also the trend of increasing the female hair loss population due to changes in dietary life, increase in stress due to social environment, etc., and the age is also getting lower.

Currently, the most frequently used hair loss treatments include finasteride (trade name Propecia®), dutasteride (trade name Avodart®), and minoxidil (trade name Minoxidil® or Rogaine®). Finasteride and dutasteride are 5α-reductase inhibitors that inhibit the conversion of testosterone to 5α-dihydrotestosterone (DHT). However, this product shows side effects such as decreased libido, erectile dysfunction, and loss of ability to drive and perform. On the other hand, in the case of minoxidil, its mechanism has not yet been fully elucidated, but as a potassium channel opener that hyperpolarizes cell membranes, it expands blood vessels and opens potassium channels, thereby providing oxygen, blood, and nutrients to hair follicles. It is believed to make hair follicles healthy by increasing their supply. However, this product also has side effects such as itchiness, erythema, skin irritation, and eye irritation at the application site, and unwanted hair growth in other parts of the body other than the head is sometimes observed. Since the side effects of these existing products can amplify patients' anxiety and lead to refusal of administration in severe cases, efforts to develop drugs with fewer side effects are continuing.

On the other hand, Samdachajo is a native Jeju region, and is a kind of grain of millet ( Setaria italica ). Although Korean Patent Registration No. 10-2150927 discloses the anti-inflammatory effect, skin moisturizing effect, skin barrier strengthening effect, and atopy improving effect of the mixed extract of Samda algae, the effect of improving hair loss of the Samda alga extract is not disclosed at all.

Therefore, the inventors of the present invention completed the present invention by discovering that the Samda perilla extract has an effect of proliferating dermal papilla cells in the process of researching a substance having excellent preventive and therapeutic effects on hair loss with few side effects.

[Prior art literature]

[Patent Literature]

(Patent Document 1) Korean Patent Registration No. 10-2150927

An object of the present invention is to provide a pharmaceutical composition for the prevention or treatment of hair loss containing an extract of cedar algae.

In addition, an object of the present invention is to provide a food composition for the prevention or improvement of hair loss containing the extract of cedar algae.

The present invention provides a pharmaceutical composition for the prevention or treatment of hair loss containing the extract of cedar algae.

In addition, the present invention provides a food composition for the prevention or improvement of hair loss containing the extract of Samdachajo.

The Samdachajo extract may be extracted with a solvent selected from the group consisting of water, C1 to C6 alcohol, ethyl acetate, hexane, chloroform, and mixed solvents thereof, but is not limited thereto.

Preferably, the Samda perilla extract of the present invention may be 0.01% to 90% ethanol extract or butanol extract, more preferably, the Samda perilla extract of the present invention may be 60% to 80% ethanol extract or butanol extract, and most preferably Preferably, the extract of Samdachajo of the present invention may be a 70% ethanol extract or a butanol extract.

The extract of Samdachajo of the present invention can prevent, treat, or improve hair loss by increasing the proliferation of dermal papilla cells.

The composition containing the extract of Samdacha algae of the present invention as an active ingredient promotes the proliferation of dermal papilla cells to induce the growth phase of hair, while having few side effects, so it can be effectively used to improve hair growth or hair loss.

Figure 1 shows the results of measuring the proliferation effect of dermal papilla cells for 70% ethanol extract of Samda perilla.

Figure 2 shows the results of measuring the proliferation effect of dermal papilla cells for the hexane extract of Samdachajo.

Figure 3 shows the results of measuring the proliferation effect of dermal papilla cells for the ethyl acetate extract of Samda perilla.

Figure 4 shows the results of measuring the proliferation effect of dermal papilla cells for the chloroform extract of Samda perilla.

Figure 5 shows the results of measuring the proliferation effect of dermal papilla cells for the butanol extract of Samda perilla.

Figure 6 shows the results of measuring the proliferation effect of dermal papilla cells on the hot water extract of Samdachajo.

Figure 7 shows the results of measuring the proliferation effect of dermal papilla cells for the perilla 70% ethanol extract.

Figure 8 shows the results of measuring the proliferation effect of dermal papilla cells on the perilla hot water extract.

Hereinafter, with reference to the accompanying drawings, embodiments and examples of the present disclosure will be described in detail so that those skilled in the art can easily practice the present invention. However, the present application may be implemented in various forms and is not limited to the embodiments and examples described herein.

Throughout the present specification, when a part "includes" a certain component, it means that it may further include other components without excluding other components unless otherwise stated.

The present invention relates to a pharmaceutical composition for the prevention or treatment of hair loss comprising an extract of cedar algae.

"Hair loss" of the present invention refers to a condition in which there is no hair in an area where hair should normally exist, regardless of the cause, and includes circular alopecia, hereditary androgenetic alopecia, telogen effluvium, traumatic alopecia, hair growth, pressure alopecia, It may be anagen hair loss, pityroid hair loss, syphilitic hair loss, seborrheic hair loss, symptomatic hair loss, scarring hair loss or congenital hair loss, but is not limited thereto.

Samda chajo ( Setaria italica Beauv. ) of the present invention is a kind of millet, which is a grain, and is a native perilla native to the Jeju region, and the variety name is Samdachal (see National Seed Resources). In the present invention, it is possible to use Samda tea, which is harvested from Jeju Island, but is not limited thereto.

The ginseng extract of the present invention can exhibit the effect of preventing or treating hair loss by promoting the proliferation of dermal papilla cells and inducing the growth phase of hair.

"Treatment" of the present invention is to improve the symptoms of hair loss by delaying or stopping the progress of hair loss by administering the composition of the present invention, or beneficially changing the symptoms of hair loss, such as promoting hair growth and hair growth, such as lengthening or increasing the number of hairs. means all actions

"Prevention" of the present invention refers to any action that suppresses or delays the onset of hair loss by administration of the composition according to the present invention, and "improvement" is related to the condition treated by administration of the composition containing the extract of the present invention. Any action that at least reduces the severity of a parameter, eg a symptom.

The "extract" of the present invention can be obtained using a conventional extraction solvent known in the art. A polar solvent or a non-polar solvent may be used as the extraction solvent. Polar solvents include water, C1 to C6 alcohols (eg, methanol, ethanol, propanol, butanol, n-propanol, iso-propanol and n-butanol, etc.), acetic acid, or mixtures of the above polar solvents. Non-polar solvents include acetone, acetonitrile, ethyl acetate, methyl acetate, butyl acetate, fluoroalkanes, hexane, ether, chloroform, dichloromethane or mixtures of the above non-polar solvents.

In one embodiment, the extract may be extracted with a solvent selected from the group consisting of water, C1 to C6 alcohol, acetic acid, and a mixed solvent thereof. Preferably, it is 0.01% to 90% ethanol extract or butanol extract, more preferably 60% to 80% ethanol extract or butanol extract, and most preferably 70% ethanol extract or butanol extract.

The extract of the present invention may be extracted through hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction, or a conventional extraction method known in the art.

The term "extract" used in the present invention means what is commonly used as a crude extract in the art, but also includes a fraction obtained by additional fractionation of the extract in a broad sense. That is, the extract includes not only those obtained using the above-described solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatographs (designed for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the purification method are also included in the crude drug extract of the present invention.

The pharmaceutical composition of the present invention can be parenterally administered or orally administered according to the desired method, and the dosage depends on the patient's weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and severity of the disease. Its range is diverse. In addition, the therapeutically effective amount of the composition may vary depending on the administration method, the target site, and the condition of the patient, and when used in the human body, the dosage should be determined in an appropriate amount in consideration of safety and efficiency.

The present invention relates to a food composition for preventing or improving hair loss comprising a samdachajo composition. The food composition may be a health functional food, dairy product, fermented product or food additive.

The "health functional food" refers to food manufactured and processed using raw materials or ingredients having useful functionalities for the human body, and "functional" refers to health uses such as adjusting nutrients for the structure and function of the human body or physiological actions, etc. It means to take it for the purpose of obtaining a beneficial effect on it.

The suitability of the above "food additive" is determined according to the specifications and standards for the item in accordance with the general rules of the Food Additive Code and general test methods approved by the Ministry of Food and Drug Safety, unless otherwise specified.

In one embodiment, the food composition is various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and It may further include salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like.

In one embodiment, the food composition includes a form such as a pill, powder, granule, precipitate, tablet, capsule, liquid, paste, gel, or jelly, and the food to which the composition can be added includes, for example, There are various types of foods, such as beverages, chewing gum, tea, vitamin complexes, health supplements, and the like.

The formulation of the food composition is not particularly limited, but may be formulated into, for example, tablets, granules, powders, liquids such as drinks, caramels, gels, bars, and the like. The food composition of each formulation can be formulated without difficulty by a person skilled in the art according to the formulation or purpose of use by selecting and blending ingredients commonly used in the field in addition to the active ingredient.

The present invention will be described in more detail through the following examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

[Example 1]

Preparation of extract

Samda-chajo 70% ethanol extract, Samda-chajo hot water extract, Samda-chajo hexane extract, Samda-chajo ethyl acetate extract, Samda-chajo chloroform extract, Samda-chajo butanol extract, Samda-chajo 70% ethanol extract, and Samda-chajo hot-water extract were prepared.

[Example 2]

Culture of rat whisker dermal papilla cells

100 units/mL penicillin-100 μg/mL streptomycin (Gibco Inc, NY, USA) and 10% heat -Incubated in DMEM (Hyclone Inc, USA) medium containing inactivated fetal bovine serum (FBS; Gibco Inc, NY, USA) in a 37°C, 5% CO ₂ incubator and subcultured once every 3 days. .

[Example 3]

Effect of 70% ethanol (EtOH) extract of Samdachajo on the proliferation of dermal papilla cells

Proliferation of rat vibrissa immortalized dermal papilla cells immortalized from dermal papilla cells isolated from rat whiskers was measured using the MTT assay. Dermal papilla cells (1.0×10 ⁴ cells/mL) were turbid in DMEM medium containing 1% FBS, placed in a 96 well plate, and cultured for 24 hours.

Samdachajo 70% ethanol extract was treated at concentrations of 1, 10 and 25 μg/mL. As a positive control, minoxidil (Sigma, MO, USA) was treated with 10 μM concentration. After culturing for 48 hours, 10 μL of WST dye (Daeil Lab Service, Seoul, Korea) was added and reacted for 3 hours. Absorbance was measured at 450 nm using a VERSAmax microplate reader (Molecular Devices, CA, USA). The average absorbance value for the sample group was obtained, and the degree of proliferation was investigated in comparison with the absorbance value of the control group, and is shown in Table 1 and FIG. 1 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	109.1
Samda Perilla 70% EtOH Extract	1 µg/mL	104.3±2.9
Samda Perilla 70% EtOH Extract	10 µg/mL	137.3±4.8
Samda Perilla 70% EtOH Extract	25 µg/mL	144.1±6.2

As shown in Table 1 and FIG. 1, when the 70% EtOH extract of Samdachajo was treated at concentrations of 1, 10, and 25 μg/mL, it was confirmed that the proliferation of dermal papilla cells increased as the concentration increased. In particular, when the Samda perilla 70% EtOH extract was treated at concentrations of 10 and 25 μg/mL, the growth of dermal papilla cells was significantly increased compared to the control group, and the dermal papilla cell proliferation effect was significantly higher than that of the positive control group. showed up

Therefore, it can be seen that the 70% ethanol extract of Samdachajo has a dermal papilla cell proliferation effect.

[Example 4]

Proliferative effect of dermal papilla cells by hexane extract

Experiments were performed on the dermal papilla cell proliferation effect of the Samda perilla hexane extract in the same manner as in Example 3, and are shown in Table 2 and FIG. 2 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	109.1
Samda tea hexane extract	1 µg/mL	100.3±6.1
Samda tea hexane extract	10 µg/mL	136.1±5.7
Samdachazo hexane extract	25 µg/mL	125.4±4.9

As shown in Table 2 and FIG. 2, it was confirmed that the proliferation of dermal papilla cells increased when the hexane extract of Samdachajo was treated at concentrations of 1, 10, and 25 μg/mL. In particular, when the hexane extract of Samda perilla extract was treated at concentrations of 10 and 25 μg/mL, the growth of dermal papilla cells increased significantly compared to the control group, and the dermal papilla cell proliferation effect was significantly higher than that of the positive control group. was

Therefore, it can be seen that the hexane extract of Samdachajo has the dermal papilla cell proliferation effect.

[Example 5]

Proliferative effect of dermal papilla cell by Ethyl Acetate (EtOAc) extract

In the same manner as in Example 3, an experiment was performed on the dermal papilla cell proliferation effect of the extract of Samda perilla ethyl acetate, and the results are shown in Table 3 and FIG. 3 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	109.1
Samda Perilla EtOAc Extract	1 µg/mL	100.7±2.2
Samda Perilla EtOAc Extract	10 µg/mL	133.8±6.0
Samda Perilla EtOAc Extract	25 µg/mL	127.9±7.1

As shown in Table 3 and FIG. 3, when the EtOAc extract of Samdachajo was treated at concentrations of 1, 10, and 25 μg/mL, it was confirmed that the proliferation of dermal papilla cells increased. In particular, when the EtOAc extract of Samda perilla extract was treated at concentrations of 10 and 25 μg/mL, the growth of dermal papilla cells significantly increased compared to the control group, and the dermal papilla cell proliferation effect was significantly higher than that of the positive control group. was

Therefore, it can be seen that the ethyl acetate extract of Samdachajo has a dermal papilla cell proliferation effect.

[Example 6]

Tertiary chloroform (CHCl ₃ ) Proliferative effect of dermal papilla cells on the extract

In the same manner as in Example 3, experiments were performed on the dermal papilla cell proliferation effect of the chloroform extract of Samda perilla extract, and are shown in Table 4 and FIG. 4 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	109.1
Samda Perilla CHCl 3 Extract	1 µg/mL	110.6±7.6
Samda Perilla CHCl 3 Extract	10 µg/mL	137.2±7.0
Samda Perilla CHCl 3 Extract	25 µg/mL	127.5±6.1

As shown in Table 4 and FIG. 4, it was confirmed that the proliferation of dermal papilla cells increased when the extract of Samdachajo CHCl ₃ was treated at concentrations of 1, 10, and 25 μg/mL. In particular, when the Samda perilla CHCl ₃ extract was treated at concentrations of 10 and 25 μg/mL, the growth of dermal papilla cells was significantly increased compared to the control group, and the dermal papilla cell proliferation effect was significantly higher than the cell proliferation measurement result of the positive control group. showed up

Therefore, it can be seen that the chloroform extract of Samda perilla has the dermal papilla cell proliferation effect.

[Example 7]

Proliferative effect of dermal papilla cells on trite perilla butanol (BuOH) extract

In the same manner as in Example 3, experiments were performed on the dermal papilla cell proliferation effect of the butanol extract of Samda perilla extract, and are shown in Table 5 and FIG. 5 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	109.1
Samda Perilla BuOH Extract	1 µg/mL	101.3±7.8
Samda Perilla BuOH Extract	10 µg/mL	134.8±5.9
Samda Perilla BuOH Extract	25 µg/mL	142.3±6.0

As shown in Table 5 and FIG. 5, when the BuOH extract of Samdachajo was treated at concentrations of 1, 10, and 25 μg/mL, it was confirmed that the proliferation of dermal papilla cells increased as the concentration increased. In particular, when the Samda perilla BuOH extract was treated at concentrations of 10 and 25 μg/mL, the growth of dermal papilla cells was significantly increased compared to the control group, and the dermal papilla cell proliferation effect was significantly higher than the cell proliferation measurement result of the positive control group. was

Therefore, it can be seen that the butanol extract of Samdachajo has the dermal papilla cell proliferation effect.

[Comparative Example 1]

Proliferative effect of dermal papilla cell on Samdachacha hot water extract

In the same manner as in Example 3, an experiment was performed on the dermal papilla cell proliferation effect of the hot water extract of Samdacha algae, and the results are shown in Table 6 and FIG. 6 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	107.0
Samdachacha hot water extract	1 µg/mL	95.3±4.8
Samdachacha hot water extract	10 µg/mL	97.8±3.9
Samdachacha hot water extract	25 µg/mL	101.0±3.9

As shown in Table 6 and FIG. 6, it was confirmed that the proliferation of dermal papilla cells was not affected when the hot water extract of Samdachajo was treated at concentrations of 1, 10, and 25 μg/mL.

[Comparative Example 2]

Effect of 70% ethanol (EtOH) extract of perilla extract on the proliferation of dermal papilla cells

Experiments on the dermal papilla cell proliferation effect of the 70% ethanol extract of perilla perilla in the same manner as in Example 3 were performed, and are shown in Table 7 and FIG. 7 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	107.2
Perilla 70% EtOH Extract	1 µg/mL	103.4±3.5
Perilla 70% EtOH Extract	10 µg/mL	103.7±4.8
Perilla 70% EtOH Extract	25 µg/mL	101.9±1.2

As shown in Table 7 and FIG. 7, it was confirmed that the proliferation of dermal papilla cells was increased when the 70% EtOH extract of perilla was treated at concentrations of 1 and 10 μg/mL, but the 70% EtOH extract of perilla at 25 μg/mL concentration When treated with, it was confirmed that the proliferation of dermal papilla cells rather decreased compared to that treated with a low concentration.

[Comparative Example 3]

Proliferative effect of dermal papilla cells on perilla hot water extract

Experiments on the dermal papilla cell proliferation effect of the perilla hot water extract were performed in the same manner as in Example 3, and are shown in Table 8 and FIG. 8 below.

	density	Cell proliferation (% of control)
control group	-	100.0
minoxidil (positive control)	10 µM	109.1
Perilla hot water extract	1 µg/mL	99.4±3.5
Perilla hot water extract	10 µg/mL	101.4±4.4
Perilla hot water extract	25 µg/mL	101.3±5.5

As shown in Table 8 and FIG. 8, when the perilla hot water extract was treated at concentrations of 1, 10, and 25 μg/mL, it was confirmed that the proliferation of dermal papilla cells was not affected.

Claims (11)

1. A pharmaceutical composition for the prevention or treatment of hair loss comprising an extract of cedar algae.
2. The prevention or treatment of hair loss according to claim 1, wherein the extract of Samdachajo is extracted with a solvent selected from the group consisting of water, C1 to C6 alcohol, ethyl acetate, hexane, chloroform, and mixed solvents thereof Pharmaceutical composition for.
3. The pharmaceutical composition for the prevention or treatment of hair loss according to claim 1, wherein the samda perilla extract is a 0.01% to 90% ethanol extract.
4. The pharmaceutical composition for the prevention or treatment of hair loss according to claim 1, wherein the samda perilla extract is a 60% to 80% ethanol extract.
5. The pharmaceutical composition for the prevention or treatment of hair loss according to claim 1, wherein the ginseng extract is a 70% ethanol extract.
6. The pharmaceutical composition for the prevention or treatment of hair loss according to claim 1, wherein the samda perilla extract is a butanol extract.
7. The pharmaceutical composition for the prevention or treatment of hair loss according to claim 1, wherein the ginseng extract increases the proliferation of dermal papilla cells.
8. A food composition for the prevention or improvement of hair loss comprising a cedar algae extract.
9. The method of claim 8, wherein the Samdachajo extract is extracted with a solvent selected from the group consisting of water, C1 to C6 alcohol, ethyl acetate, hexane, chloroform, and mixed solvents thereof. For preventing or improving hair loss, characterized in that food composition.
10. [Claim 9] The food composition for preventing or improving hair loss according to claim 8, wherein the extract of Samdachajo is 0.01% to 90% ethanol extract.
11. [Claim 9] The food composition for the prevention or improvement of hair loss according to claim 8, wherein the samda perilla extract is a butanol extract.

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