WO2023045571A1 - Appareil de mesure de niveau de liquide et méthode d'utilisation dans un dispositif de culture, dispositif de culture et support - Google Patents

Appareil de mesure de niveau de liquide et méthode d'utilisation dans un dispositif de culture, dispositif de culture et support Download PDF

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WO2023045571A1
WO2023045571A1 PCT/CN2022/109728 CN2022109728W WO2023045571A1 WO 2023045571 A1 WO2023045571 A1 WO 2023045571A1 CN 2022109728 W CN2022109728 W CN 2022109728W WO 2023045571 A1 WO2023045571 A1 WO 2023045571A1
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Prior art keywords
liquid level
probe assembly
detection
signal
level detection
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PCT/CN2022/109728
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English (en)
Chinese (zh)
Inventor
陈欢
段泽鹏
胡伟
鞠焕文
王潘飞
陈海涛
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青岛海尔生物医疗科技有限公司
青岛海尔生物医疗股份有限公司
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Publication of WO2023045571A1 publication Critical patent/WO2023045571A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
    • G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the present application relates to the technical field of liquid level detection, for example, to a liquid level detection device and method for culture equipment, culture equipment and media.
  • the carbon dioxide incubator belongs to precision medical equipment, which can provide suitable temperature, humidity and carbon dioxide concentration to realize the cultivation of human tissues and cells.
  • the water level of the pure water is relatively low, usually, the water level is about 2 cm. Due to the low content of impurity ions in pure water and the low water level, it is difficult to detect the liquid level in the carbon dioxide incubator.
  • the existing way to realize the liquid level detection in the carbon dioxide incubator is to set up a conductivity type liquid level module, which has a first output terminal and a second output terminal, the first output terminal is used to output a square wave signal, and the second output terminal outputs Switching value, the liquid level of pure water in the incubator can be indirectly known through the second output terminal.
  • the square wave signal is a direct current signal.
  • the square wave signal is a DC signal, which can easily cause the probe to rust and age, affecting the accuracy of liquid level detection.
  • Embodiments of the present disclosure provide a liquid level detection device and method for culture equipment, culture equipment and media, so as to improve the accuracy of liquid level detection.
  • the liquid level detection device includes: a probe assembly, which can be immersed in the culture device; a micro-processing unit, which is electrically connected to the probe assembly and constitutes a detection circuit, configured to The probe component inputs a sine wave signal to trigger the conduction of the detection loop, and outputs a detection signal corresponding to the conduction state of the detection loop.
  • the culture device contains liquid, and the device includes the aforementioned liquid level detection device for the culture device.
  • the liquid level detection method includes: inputting a sine wave signal to the probe assembly to trigger conduction of the detection loop; outputting a detection signal corresponding to the conduction state of the detection loop.
  • the storage medium stores program instructions, and when the program instructions are executed, the aforementioned liquid level detection method for a culture device is executed.
  • the liquid level detection device, method, cultivation equipment and medium for cultivation equipment provided by the embodiments of the present disclosure can achieve the following technical effects:
  • the detection circuit By inputting a sine wave signal to the probe assembly, the detection circuit is turned on when the probe assembly is immersed in the liquid of the culture device, and a detection signal corresponding to the conduction state of the detection circuit is output, so as to obtain the temperature of the culture device according to the detection signal Liquid level situation. Since the sine wave signal is an AC signal, the sine wave signal input to the probe assembly can weaken the electrolysis of impurity ions in the pure water. Therefore, the device can prevent the probe assembly from rusting and aging, and improve the accuracy of liquid level detection. Spend.
  • FIG. 1 is a schematic diagram of a liquid level detection device used in culture equipment provided by an embodiment of the present disclosure
  • Fig. 2 is a schematic diagram of a liquid level detection device used in culture equipment provided by an embodiment of the present disclosure
  • Fig. 3 is a schematic diagram of another liquid level detection device used in culture equipment provided by an embodiment of the present disclosure.
  • Fig. 4 is a schematic diagram of another liquid level detection device used in culture equipment provided by an embodiment of the present disclosure.
  • Fig. 5 is a schematic diagram of a liquid level detection method for a culture device provided by an embodiment of the present disclosure
  • Fig. 6 is a schematic diagram of another liquid level detection method for culture equipment provided by an embodiment of the present disclosure.
  • Fig. 7 is a schematic diagram of a liquid level detection device used in culture equipment provided by an embodiment of the present disclosure.
  • Fig. 8 is a schematic diagram of another liquid level detection device used in a culture device provided by an embodiment of the present disclosure.
  • A/B means: A or B.
  • a and/or B means: A or B, or, A and B, these three relationships.
  • correspondence may refer to an association relationship or a binding relationship, and the correspondence between A and B means that there is an association relationship or a binding relationship between A and B.
  • an embodiment of the present disclosure provides a liquid level detection device for culture equipment, including a probe assembly 10 and a microprocessing unit 20 .
  • Probe assembly 10 may be submerged within a culture device.
  • the micro-processing unit 20 is electrically connected with the probe assembly 10 and constitutes a detection circuit, configured to input a sine wave signal to the probe assembly 10 to trigger conduction of the detection circuit, and output a detection signal corresponding to the conduction state of the detection circuit.
  • the detection circuit is turned on when the probe assembly is immersed in the liquid of the culture equipment, and the output is connected to the detection circuit.
  • the detection signal corresponding to the on-state, so as to obtain the liquid level of the culture device according to the detection signal. Since the sine wave signal is an AC signal, the sine wave signal input to the probe assembly can weaken the electrolysis of impurity ions in the pure water. Therefore, the device can prevent the probe assembly from rusting and aging, and improve the accuracy of liquid level detection. Spend.
  • the microprocessing unit 20 includes a bipolar power supply circuit 201 and a signal converter 202 .
  • the bipolar power supply circuit 201 is used to generate bipolar voltage signals.
  • the signal converter 202 is electrically connected to the bipolar power supply circuit 201 , and is used to convert the bipolar voltage signal into a sine wave signal and input it to the probe assembly 10 .
  • a bipolar voltage signal with a stable amplitude can be generated, and by using a signal converter, the bipolar voltage signal can be converted into a bipolar voltage signal A sine wave signal of the same frequency as the signal, so that a stable sine wave signal can be supplied to the probe assembly.
  • the sine wave signal and the bipolar voltage signal have the same frequency, by adjusting the control parameters of the bipolar power supply circuit, the frequency adjustment of the bipolar voltage signal can be realized, thereby realizing the adjustment of the frequency of the sine wave signal and improving the frequency Regulatory efficiency.
  • the bipolar power supply circuit 201 includes a DC-DC (Direct current-Direct current) power supply module 2011 and a single-chip microcomputer 2012.
  • the DC-DC power supply module 2011 is used to generate a negative voltage signal.
  • the single chip microcomputer 2012 is used to generate a square wave signal, and the square wave signal and the negative pressure signal form a bipolar voltage signal.
  • the bipolar power supply circuit uses a DC-DC power supply module to generate a negative voltage signal, and a single-chip microcomputer to generate a square wave signal, which can provide a bipolar voltage signal to the signal converter for the signal converter to convert the bipolar voltage signal Perform waveform conversion.
  • the DC-DC power module 201 adopts a power module with a model number of MAX1852.
  • the signal converter 202 adopts a dual operational amplifier modeled as LM358.
  • the micro-processing unit 20 includes a bipolar power supply circuit 201 and a signal converter 202 .
  • the bipolar power supply circuit 201 is used to generate bipolar voltage signals.
  • the signal converter 202 is electrically connected to the bipolar power supply circuit 201 for converting the bipolar voltage signal into a sine wave signal for input to the probe assembly 10 .
  • the bipolar power supply circuit 201 includes a DC-DC power supply module 2011 and a single chip microcomputer 2012 .
  • the DC-DC power supply module 2011 is used to generate a negative voltage signal.
  • the single chip microcomputer 2012 is used to generate a square wave signal, and the square wave signal and the negative pressure signal form a bipolar voltage signal.
  • the micro-processing unit 20 is also configured to obtain the distance information of the probe assembly 10, obtain the target frequency corresponding to the distance information according to the preset first correspondence, and adjust the frequency of the square wave signal with the target frequency.
  • the voltage signal required by the probe configured by the traditional conductivity type liquid level module is a fixed value, and the existing square wave signal belongs to the DC signal. If the DC signal does not match the aforementioned voltage signal, it can strengthen the impurity ion in the pure water. of electrolysis.
  • the liquid contained in the culture equipment is usually pure water.
  • the AC signal can weaken the electrolysis of impurity ions in the pure water, thus avoiding the probe
  • the needle assembly is rusted and aged, therefore, the first corresponding relationship between the spacing information of the probe assembly and the frequency of the square wave signal can be preset, and the target frequency corresponding to the spacing information is matched from the first corresponding relationship, and the The target frequency adjusts the frequency of the square wave signal, thereby effectively weakening the electrolysis of impurity ions in pure water.
  • the spacing information of the probe components is different, and the frequency and amplitude of the corresponding square wave signals are also different. Therefore, the correspondence between the preset spacing information and frequency, and Or, the preset correspondence between the distance information and the amplitude can realize the intelligent adjustment of the frequency and/or amplitude of the square wave signal according to the distance information of the probe assembly.
  • the preset first corresponding relationship may be that the spacing information of the probe assembly has a linear corresponding relationship with the frequency of the square wave signal.
  • the target frequency value is 33 Hz (Hertz).
  • the target frequency value is 40 Hz.
  • the frequency adjustment of the square wave signal can be realized, and the sine wave signal and the square wave signal have the same frequency, thereby realizing the frequency adjustment of the sine wave signal and effectively avoiding the aging of the probe assembly Rust happens.
  • the probe assembly is usually configured with two electrodes, and the two electrodes are spaced apart. Since there are various types of probe components and the distance between the two electrodes of different types of probe components is different, the device realizes the automatic matching between the frequency of the sine wave signal and the distance between the probe components, reducing the requirements for the selection of the probe components. This makes the adaptability of the device better.
  • an embodiment of the present disclosure also provides a liquid level detection device for culture equipment, including a probe assembly 10 , a microprocessing unit 20 , an energy storage capacitor 30 and a rectification circuit 40 .
  • the probe assembly 10 is submersible into the culture device and has a detection end C.
  • the micro-processing unit 20 is electrically connected with the probe assembly 10 and constitutes a detection circuit, configured to input a sine wave signal to the probe assembly 10 to trigger conduction of the detection circuit, and output a detection signal corresponding to the conduction state of the detection circuit.
  • the micro-processing unit 20 outputs a voltage signal through the detection terminal C.
  • the microprocessing unit 20 includes a bipolar power supply circuit 201 and a signal converter 202 .
  • the bipolar power supply circuit 201 is used to generate bipolar voltage signals.
  • the signal converter 202 is electrically connected to the bipolar power supply circuit 201 , and is used to convert the bipolar voltage signal into a sine wave signal and input it to the probe assembly 10 .
  • the energy storage capacitor 30 is electrically connected to the signal converter 202 and the probe assembly 10 .
  • the rectification circuit 40 is electrically connected to the detection terminal C of the probe assembly 10 for rectifying the voltage signal of the detection terminal C to generate a detection signal.
  • the probe assembly when the culture equipment has no liquid, the probe assembly is in an open circuit state.
  • the energy storage capacitor is equivalent to an AC voltage source.
  • the AC signal provided by the energy storage capacitor is processed by half-wave rectification, thereby generating a detection signal corresponding to the culture equipment without liquid, and then accurately knowing the liquid level of the pure water in the culture equipment.
  • An embodiment of the present disclosure provides a liquid level detection device for culture equipment, including a probe assembly 10 and a microprocessing unit 20 .
  • Probe assembly 10 may be submerged within a culture device.
  • the micro-processing unit 20 is electrically connected to the probe assembly 10 and constitutes a detection circuit, configured to input a sine wave signal to the probe assembly 10 to trigger conduction of the detection circuit, and output a detection signal corresponding to the conduction state of the detection circuit, And according to the preset second corresponding relationship, the target detection frequency corresponding to the conduction state is obtained, and the liquid level detection is performed through the probe assembly according to the target detection frequency.
  • the micro-processing unit can preset a second corresponding relationship, and the second corresponding relationship
  • the correlation between the conduction state of the detection circuit and the detection frequency is pre-stored, and the target detection frequency corresponding to the conduction state is obtained from the second corresponding relationship, so as to detect the liquid level through the probe assembly according to the target detection frequency, The effectiveness and real-time performance of liquid level detection are improved.
  • the detection circuit when there is pure water in the cultivation equipment, the detection circuit is in a conducting state, at this time, the liquid level detection is performed at the first preset detection frequency to avoid rust and aging caused by continuous liquid level detection the occurrence of the situation.
  • the detection circuit is in a disconnected state, and at this time, the liquid level detection is performed at the second preset detection frequency.
  • the first preset detection frequency is lower than the second preset detection frequency.
  • an embodiment of the present disclosure provides a liquid level detection method for culture equipment, including:
  • the culture equipment inputs a sine wave signal to the probe assembly to trigger conduction of the detection circuit.
  • the cultivation device outputs a detection signal corresponding to the conduction state of the detection circuit.
  • liquid level detection method for cultivating equipment provided by the embodiments of the present disclosure, rusting and aging of the probe assembly can be avoided, and the accuracy of liquid level detection can be improved.
  • an embodiment of the present disclosure also provides a liquid level detection method for culture equipment, including:
  • the cultivating device obtains the spacing information of the probe components.
  • the cultivation device obtains the target frequency corresponding to the distance information according to the preset first correspondence relationship.
  • the training device adjusts the frequency of the square wave signal at the target frequency.
  • the culture device inputs a sine wave signal to the probe assembly to trigger conduction of the detection circuit.
  • the cultivation device outputs a detection signal corresponding to the conduction state of the detection circuit.
  • the AC signal can weaken the electrolysis of impurity ions in pure water only when the spacing information of the probe assembly matches the frequency of the square wave signal, and then To avoid rusting and aging of the probe assembly, therefore, the first correspondence between the spacing information of the probe assembly and the frequency of the square wave signal can be preset, and the target frequency corresponding to the spacing information can be matched from the first correspondence, And adjust the square wave signal with the target frequency, so as to effectively weaken the electrolysis of impurity ions in pure water. At the same time, after the training equipment adjusts the frequency of the square wave signal to the target frequency, the intelligent matching between the square wave signal and the spacing information of the probe components can be realized.
  • the distance information between the two electrodes of the probe assembly is a fixed value.
  • the distance information between the two electrodes will increase due to the electrolysis of impurity ions in pure water. Increase/decrease, or when the type of the probe unit is changed, the pitch information of the new probe unit is changed.
  • intelligent matching between the frequency of the square wave signal and the new distance information can be realized according to the preset first corresponding relationship.
  • the micro-processing unit includes a bipolar power supply circuit and a signal converter.
  • the bipolar power supply circuit includes a DC-DC power supply module and a single-chip microcomputer.
  • the signal converter is electrically connected to the energy storage capacitor C62 and is electrically connected to the probe assembly through the terminal CN10.
  • the probe assembly is electrically connected to diode D2.
  • the diode D2 has a half-wave rectification function.
  • the C terminal of the probe assembly is the detection terminal C.
  • the cathode terminal of the diode D2 is the output terminal D after half-wave rectification.
  • the DC-DC power supply module MAX1852 generates -5V negative voltage signal, and the single-chip microcomputer can generate +5V square wave signal.
  • the DC-DC power supply module MAX1852 is used to provide a negative pressure signal to the -5V negative pressure port of the signal converter LM358, and the microcontroller supplies a square wave signal to the signal conversion circuit LM358 through the branch where the capacitor C61 is located.
  • the signal conversion circuit LM358 converts the bipolar voltage signal formed by the received negative pressure signal and square wave signal into a sine wave signal and inputs it to the probe assembly.
  • the detection steps of the liquid level detection device used for cultivating equipment are as follows:
  • the DC-DC power supply module generates a -5V negative voltage signal
  • the single-chip microcomputer generates a +5V square wave signal
  • the signal converter converts the received bipolar voltage signal to generate a signal with the same frequency as the bipolar voltage signal -4 ⁇ +4V sine wave signal.
  • the half-wave rectification signal is obtained through the output terminal D, it is determined that the detection circuit is disconnected, and there is no pure water in the cultivation device.
  • liquid level detection is performed with a second preset detection period of 15 seconds.
  • the liquid level detection is performed with a first preset detection period of 30 minutes. Wherein, the first preset detection period is longer than the second preset detection period.
  • an embodiment of the present disclosure provides a liquid level detection device for culture equipment, including a processor (processor) 100 and a memory (memory) 101 .
  • the device may also include a communication interface (Communication Interface) 102 and a bus 103.
  • Communication interface 102 may be used for information transfer.
  • the processor 100 can call the logic instructions in the memory 101 to execute the method for detecting the liquid level of the cultivation device in the above embodiment.
  • the above logic instructions in the memory 101 may be implemented in the form of software functional units and may be stored in a computer-readable storage medium when sold or used as an independent product.
  • the memory 101 can be used to store software programs and computer-executable programs, such as program instructions/modules corresponding to the methods in the embodiments of the present disclosure.
  • the processor 100 executes the program instructions/modules stored in the memory 101 to execute functional applications and data processing, that is, to realize the liquid level detection method for the culture device in the above-mentioned embodiments.
  • the memory 101 may include a program storage area and a data storage area, wherein the program storage area may store an operating system and an application program required by at least one function; the data storage area may store data created according to the use of the terminal device, and the like.
  • the memory 101 may include a high-speed random access memory, and may also include a non-volatile memory.
  • An embodiment of the present disclosure provides a culture device that accommodates liquid, and the culture device includes a liquid level detection device for the culture device.
  • the liquid level detection device for culture equipment includes a probe assembly 10 and a micro-processing unit 20 .
  • Probe assembly 10 may be submerged within a culture device.
  • the micro-processing unit 20 is electrically connected with the probe assembly 10 and constitutes a detection circuit, configured to input a sine wave signal to the probe assembly 10 to trigger conduction of the detection circuit, and output a detection signal corresponding to the conduction state of the detection circuit.
  • the cultivation equipment provided by the embodiments of the present disclosure can prevent the probe assembly from rusting and aging, and improve the accuracy of liquid level detection.
  • An embodiment of the present disclosure provides a computer-readable storage medium, which stores computer-executable instructions, and the computer-executable instructions are configured to execute the above liquid level detection method for a culture device.
  • An embodiment of the present disclosure provides a computer program product, the computer program product includes a computer program stored on a computer-readable storage medium, the computer program includes program instructions, and when the program instructions are executed by a computer, the The computer executes the above-mentioned liquid level detection method for cultivating equipment.
  • the above-mentioned computer-readable storage medium may be a transitory computer-readable storage medium, or a non-transitory computer-readable storage medium.
  • the technical solutions of the embodiments of the present disclosure can be embodied in the form of software products, which are stored in a storage medium and include one or more instructions to make a computer device (which can be a personal computer, a server, or a network equipment, etc.) to perform all or part of the steps of the method described in the embodiments of the present disclosure.
  • the aforementioned storage medium can be a non-transitory storage medium, including: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), magnetic disk or optical disc, etc.
  • the term “and/or” as used in this application is meant to include any and all possible combinations of one or more of the associated listed ones.
  • the term “comprise” and its variants “comprises” and/or comprising (comprising) etc. refer to stated features, integers, steps, operations, elements, and/or The presence of a component does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and/or groupings of these.
  • an element defined by the statement “comprising a " does not exclude the presence of additional identical elements in the process, method or apparatus comprising said element.
  • the disclosed methods and products can be implemented in other ways.
  • the device embodiments described above are only illustrative.
  • the division of the units may only be a logical function division.
  • multiple units or components may be combined Or it can be integrated into another system, or some features can be ignored, or not implemented.
  • the mutual coupling or direct coupling or communication connection shown or discussed may be through some interfaces, and the indirect coupling or communication connection of devices or units may be in electrical, mechanical or other forms.
  • each functional unit in the embodiments of the present disclosure may be integrated into one processing unit, each unit may exist separately physically, or two or more units may be integrated into one unit.
  • each block in a flowchart or block diagram may represent a module, program segment, or part of code that includes one or more Executable instructions.
  • the functions noted in the block may occur out of the order noted in the figures.
  • two blocks in succession may, in fact, be executed substantially concurrently, or they may sometimes be executed in the reverse order, depending upon the functionality involved.
  • the operations or steps corresponding to different blocks may also occur in a different order than that disclosed in the description, and sometimes there is no specific agreement between different operations or steps.
  • each block in the block diagrams and/or flowcharts, and combinations of blocks in the block diagrams and/or flowcharts can be implemented by a dedicated hardware-based system that performs the specified function or action, or can be implemented by dedicated hardware implemented in combination with computer instructions.

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Abstract

Un appareil de mesure de niveau de liquide destiné à être utilisé dans un dispositif de culture, comprenant un ensemble sonde (10), immergé dans le dispositif de culture ; et une unité de microtraitement (20), connectée électriquement à l'ensemble sonde (10) afin de former un circuit de mesure, et configurée pour entrer un signal d'onde sinusoïdale dans l'ensemble sonde (10), de manière à déclencher la connexion du circuit de mesure et émettre en sortie un signal de mesure correspondant à un état connecté du circuit de mesure. L'invention comprend également un procédé de mesure de niveau de liquide destiné à être utilisé dans un dispositif de culture, un dispositif de culture et un support de stockage.
PCT/CN2022/109728 2021-09-22 2022-08-02 Appareil de mesure de niveau de liquide et méthode d'utilisation dans un dispositif de culture, dispositif de culture et support WO2023045571A1 (fr)

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