WO2023016776A1 - Plaque de microtitration - Google Patents

Plaque de microtitration Download PDF

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Publication number
WO2023016776A1
WO2023016776A1 PCT/EP2022/070608 EP2022070608W WO2023016776A1 WO 2023016776 A1 WO2023016776 A1 WO 2023016776A1 EP 2022070608 W EP2022070608 W EP 2022070608W WO 2023016776 A1 WO2023016776 A1 WO 2023016776A1
Authority
WO
WIPO (PCT)
Prior art keywords
samples
sensor component
cover
microtiter plate
base plate
Prior art date
Application number
PCT/EP2022/070608
Other languages
German (de)
English (en)
Inventor
Thomas Moore
Daniel Weigel
Mohammad Sadegh Ebrahimi
Original Assignee
Analytik Jena Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Analytik Jena Gmbh filed Critical Analytik Jena Gmbh
Publication of WO2023016776A1 publication Critical patent/WO2023016776A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/24—Arrangements or instruments for measuring magnetic variables involving magnetic resonance for measuring direction or magnitude of magnetic fields or magnetic flux
    • G01R33/26—Arrangements or instruments for measuring magnetic variables involving magnetic resonance for measuring direction or magnitude of magnetic fields or magnetic flux using optical pumping
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/28—Details of apparatus provided for in groups G01R33/44 - G01R33/64
    • G01R33/30—Sample handling arrangements, e.g. sample cells, spinning mechanisms
    • G01R33/302—Miniaturized sample handling arrangements for sampling small quantities, e.g. flow-through microfluidic NMR chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
    • G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance

Definitions

  • the invention relates to a microtiter plate and a measuring device for the analysis of a large number of liquid or pasty samples provided as drops using nuclear magnetic resonance.
  • Nuclear magnetic resonance spectroscopy allows the investigation of the electronic environment of individual atoms and their interactions with neighboring atoms.
  • the components of samples and the structures of molecules can be determined using NMR spectroscopy.
  • NMR spectroscopy also forms the basis of magnetic resonance tomography, which is often used in the medical or biological field to examine tissues and organs.
  • atomic nuclei have a non-zero nuclear spin and thus a magnetic moment as rotating charge carriers, such as 1 H or 13 C atoms.
  • the nuclear spins perform a precession movement, the so-called Larmor precession, around the axis of the constant magnetic field.
  • the atomic nuclei change the orientation of their nuclear spins to the magnetic field through the absorption or emission of alternating magnetic fields when these are resonant with the Larmor frequency. This effect is also known as nuclear magnetic resonance.
  • the possible magnetic angular momentum quantum states of the nuclear spins are equidistant and dependent on the Larmor frequency.
  • the frequency and duration of the Larmor precession depend on the respective nuclear spin and its spatial and chemical environment. The detection of the Larmor precessions based on the Larmor frequencies thus enables a very precise determination of the chemical composition of the sample and the spatial structure of the molecules contained in the sample.
  • the alternating magnetic field is usually generated by a magnetic coil.
  • inductive methods are often used to detect nuclear magnetic resonances.
  • the sample is often surrounded by an induction coil in which an electrical voltage is generated by the alternating magnetic fields emitted by the nuclear spins occurring.
  • strong static magnetic fields of up to 25 T are used to polarize the nuclear spins in order to obtain a preferred polarization of nuclear spins aligned in the same way and thus a magnetization that can be measured with conventional magnetic field sensors.
  • miniaturization of the NMR measuring devices is not possible.
  • the sample to be examined is usually placed in a long glass tube.
  • the required sample volume is a few milliliters.
  • this is a major disadvantage, particularly in the life sciences sector, since the available volumes of the Samples are often in the microhter range. For example, often only a few microliters of sample can be extracted from cell cultures or tissue points.
  • a newer generation of magnetic field sensors falls into the field of so-called quantum sensors, in which a wide variety of quantum effects are used to determine various physical and/or chemical measured variables.
  • quantum sensors in which a wide variety of quantum effects are used to determine various physical and/or chemical measured variables.
  • approaches are of particular interest with regard to the increasing efforts towards miniaturization while at the same time increasing the performance of the respective sensors.
  • Quantum sensors are based on the fact that certain quantum states of individual atoms can be very precisely controlled and read out. In this way, for example, precise and low-interference measurements of electric and/or magnetic fields and gravitational fields with resolutions in the nanometer range are possible.
  • various spin-based sensor arrangements have become known, for which atomic transitions in crystal bodies are used to detect changes in movements, electric and/or magnetic fields or gravitational fields.
  • various systems based on quantum-optical effects have also become known, such as quantum gravimeters or optically pumped magnetometers, the latter in particular being based on gas cells, among other things.
  • various devices which use atomic transitions, for example in various crystal bodies, in order to detect even small changes in movements, electric and/or magnetic fields or gravitational fields.
  • diamond with at least one silicon or nitrogen defect center, silicon carbide with at least one silicon defect or hexagonal boron nitride with at least one defect color center is used as the crystal body.
  • the crystal bodies can have one or more defects.
  • gas cells in which atomic transitions and spin states can be queried optically to determine magnetic and/or electrical properties, among other things.
  • a gaseous alkali metal and a buffer gas are usually present in the gas cell.
  • Magnetic properties of a surrounding medium can be determined by Rydberg states generated in the gas cell.
  • gas cells are used in quantum-based standards, which measure physical quantities with high precision provide. They have long been used in frequency standards or atomic clocks, as is known from EP 0 550 240 B1.
  • a microtiter plate for the analysis of a large number of liquid or pasty samples provided as drops using nuclear magnetic resonance of the samples comprising a base plate with a large number of depressions made in the base plate or a large number of first elevations for receiving the samples , a cover, which is designed in such a way that the samples can be enclosed between the depressions or the first elevations of the base plate and the cover, and at least one sensor component, which is designed to emit a light influenced by the nuclear magnetic resonances of the samples under an excitation light , wherein the at least one sensor component is at least partially in contact with the sample and forms at least part of the base plate and/or the cover, the base plate and/or the cover being at least in the region of the at least one sensor component for the excitation light and the emitted light are transparent.
  • the at least one sensor component When excited with an excitation light, the at least one sensor component emits a light which is influenced, in particular dependent, on the nuclear spin resonances of the respective sample.
  • the light is, for example, a fluorescent light.
  • the intensity of the light or a relative distance between two intensity peaks is influenced by the nuclear magnetic resonance of the respective sample.
  • the emitted light is detected outside of the microtiter plate, at least one chemical and/or physical property of the respective sample being determined on the basis of the detected light.
  • the at least one sensor component is part of the base plate and/or the cover.
  • the at least one sensor component can be configured in one piece or in multiple pieces; in both cases the at least one sensor component is in contact with each of the samples.
  • the at least one sensor component is at least one crystal body with at least one defect. With appropriate optical excitation, crystal bodies with at least one defect show a fluorescence signal which is dependent, among other things, on a magnetic field present on the crystal body.
  • the nuclear spin resonances of the respective sample influence the magnetic field applied to the at least one sensor component, so that at least one chemical and/or physical property of the respective sample can be determined using the fluorescence signal.
  • the crystal body with the at least one defect leads to an improvement in the measurement accuracy of the detection of the nuclear magnetic resonances of the respective sample and thus the at least one chemical and/or physical property of the respective sample due to its high sensitivity to magnetic fields.
  • the fluorescence signal in particular the intensity of the fluorescence signal, can be used to determine the magnetic flux density, the magnetic susceptibility, the magnetic permeability or another variable related to at least one of these variables.
  • the crystal body is a diamond with at least one silicon vacancy center or nitrogen vacancy center, silicon carbide with at least one silicon vacancy center or hexagonal boron nitride with at least one vacancy color center.
  • the at least one sensor component is preferably designed as a layer, with the layer being arranged in the area of the cover and/or in the area of the base plate.
  • the layer is applied using a CVD or PVD method.
  • the layer can be designed as a single, continuous layer which extends across all samples, or one layer can be applied in the respective areas of the samples.
  • the at least one sensor component is advantageously designed as a layer, with the crystal body being arranged in the area of the cover and/or in the area of the base plate, with the at least one defect being arranged in an area of the crystal body facing the samples. Since the at least one defect emits light that is influenced by the nuclear spin resonances of the respective sample, it must be arranged adjacent to the samples.
  • the cover is made of glass, with the at least one sensor component being applied as a layer to a surface of the cover facing the base plate.
  • the lid has a large number of second elevations, the second elevations being cup-like or dome-like, such that the second elevations can be inserted into the depressions in the base plate or such that the samples can be positioned between the first elevations and the second elevations .
  • very small volumes of the respective sample are thus clamped and held between the first elevations and the second elevations or between the depressions and the second elevations.
  • the depressions, the first elevations, the cover and/or the second elevations are designed in a defined area in such a way that the samples, due to their hydrophilicity or their hydrophobicity or lipophilicity/lipophobia and/or ultraphobia and/or their surface tension in the defined area can be positioned.
  • the defined area is equipped with a property, for example, which conflicts with a property of the cover and/or the base plate.
  • the respective sample is thus preferably retained in the defined area.
  • the bottom plate and its first elevations or depressions can have hydrophilic properties, whereas the defined area has lipophilic properties. A sample with more lipophilic properties will therefore preferentially arrange itself in the defined area.
  • a hydrophilic sample such as an aqueous sample
  • the properties of the bottom plate and the defined area could be reversed accordingly. Ultraphobic properties repel both hydrophilic and lipophilic samples.
  • At least one spacer is provided, with the at least one spacer being designed in such a way that a defined layer thickness of the samples can be set between the cover and the base plate.
  • the base plate can preferably be produced from a polymer resin by means of an injection molding process and/or an embossing process.
  • the at least one sensor component is usually arranged on the microtiter plate in a separate step.
  • the samples each have a volume of less than 100 pl, in particular less than 10 pl.
  • a measuring device for the analysis of a large number of liquid or pasty samples provided as drops using nuclear magnetic resonances of the samples, comprising a holder for receiving a microtiter plate according to at least one of the preceding claims, at least one excitation unit for exciting the at least one sensor component by means of the excitation light, at least one detection unit for detecting the light emitted by the at least one sensor component, and an evaluation unit which, based on the detected light determines at least one chemical and / or physical property of the samples.
  • the microtiter plate is placed in a holder which encompasses the microtiter plate, for example, on one or more of its side surfaces and/or its bottom surfaces.
  • the at least one excitation unit emits an excitation light, which is guided to the at least one sensor component.
  • the light emitted by the at least one sensor component is guided to the at least one detection unit.
  • at least one optical element can optionally be used, such as a mirror, a prism, a filter, or an optical fiber.
  • the emitted light which is influenced by the respective nuclear spin resonances of the sample, is detected separately.
  • a single excitation unit and a single detection unit can be used, for example, which individually excite the respective samples one after the other and detect the emitted light by making either the microtiter plate and/or the excitation unit and the detection unit movable.
  • a large number of excitation units and detection units can be used in order to be able to analyze a defined number of samples at the same time.
  • an alternating frequency source is provided for exciting the at least one sensor component and/or the samples.
  • the introduction of alternating fields can be used to carry out typical excitation-query sequences known from NMR spectroscopy, such as the spin-echo method or the so-called XY8N sequence, in which the magnetization of the nuclear spins is specifically adjusted and observed using the alternating field becomes.
  • NMR spectroscopy such as the spin-echo method or the so-called XY8N sequence
  • rapidly changing magnetic fields can also be precisely detected by different components of the respective sample that are present in the area of the at least one sensor component.
  • the respective sample can also be excited using the alternating field in order to increase the sensitivity of the analysis of the nuclear magnetic resonances.
  • the alternating frequency antenna can be a microwave antenna, for example.
  • an inductor is provided, which is designed to induce a preferential polarization of the nuclear spins of the samples.
  • the inductor is a magnetic field device that generates a magnetic field at least in a region of the samples and in a region of the at least one sensor component.
  • the magnetic field is in particular static.
  • the inductor can be based on other methods of hyperpolarization, for example in the form of para-hydrogen introduced into the samples.
  • the excitation unit can be used as the laser source.
  • the first and/or second microwave source can be used both to induce a preferred polarization of the nuclear spins of the samples and to excite the at least one sensor component.
  • FIGS. 1-6 show:
  • Fig. 1 A simplified energy scheme for a negatively charged NV center in diamond.
  • 3a-b a second embodiment of the microtiter plate according to the invention.
  • FIG. 1 A simplified energy scheme for a negatively charged nitrogen vacancy center (NV center) in a diamond is shown in FIG. 1 in order to exemplarily explain the excitation and the fluorescence of a vacancy in a crystal II body.
  • the following considerations can be transferred to other crystal bodies with corresponding defects.
  • each carbon atom is typically covalently bonded to four other carbon atoms.
  • a nitrogen vacancy center (NV center) consists of a defect in the diamond lattice, i.e. an unoccupied lattice site, and a nitrogen atom as one of the four neighboring atoms.
  • the negatively charged NV- centers are important for the excitation and evaluation of fluorescence signals.
  • Excitation light 1 from the green range of the visible spectrum e.g. excitation light 1 with a wavelength of 532 nm, excites an electron from the ground state 3 A into a vibrational state of the excited state 3 E, which emits a fluorescence photon 2 returns to the 3 A ground state with a wavelength of 630 nm.
  • This fluorescence signal is a measure of the zero field splitting A and can be used to determine and/or monitor the temperature T.
  • An applied magnetic field with a magnetic field strength B leads to a splitting (Zeeman splitting) of the magnetic sub-states, so that the ground state consists of three energetically separated sub-states, each of which can be excited.
  • the intensity of the fluorescence signal depends on the respective magnetic substate from which the excitation took place, so that the distance between the fluorescence minima can be used, for example, to calculate the magnetic field strength B using the Zeeman formula.
  • the magnetic field strength B is modified by the nuclear spins of the respective sample 4 or results from them.
  • evaluating the fluorescence signal are provided within the scope of the present invention, such as evaluating the intensity of the fluorescent light, which is also proportional to the applied magnetic field.
  • An electrical evaluation in turn, can be carried out, for example, via photocurrent detection of magnetic resonance (PDMR for short).
  • PDMR photocurrent detection of magnetic resonance
  • FIGS. 2a-b A first embodiment of the microtiter plate 3 according to the invention is shown in FIGS. 2a-b.
  • the bottom plate 5 has a Variety of first surveys 7 and the cover 8 has a plurality of second surveys 10 between which the samples 4 are added.
  • the samples each have a volume of less than 100 pl, in particular less than 10 pl.
  • the second elevations 10 are designed in the manner of a dome, for example, alternatively they are designed in the manner of a bowl.
  • at least one spacer 12 is arranged between the cover 8 and the base plate 5, which is designed in such a way that a defined layer thickness 13 of the samples 4 between the cover 8 and the base plate 5 can be set.
  • the base plate 5 is produced from a polymer resin, for example by means of an injection molding process and/or an embossing process.
  • the at least one sensor component 9 is configured as a multiplicity of sensor components 9, for example.
  • the sensor component 9 forms part of the second elevations 10 of the cover 8 and is at least partially in contact with the samples 4 when the microtiter plate is closed, i.e. when the cover 8 is placed on the base plate 5, so that the sensor components 9 are one of the Nuclear magnetic resonances of the respective sample 4 emit light influenced.
  • the at least one sensor component is, for example, at least one crystal II body with at least one defect, such as in particular a diamond with at least one silicon defect center or nitrogen defect center, silicon carbide with at least one silicon defect center or hexagonal boron nitride with at least one void color center.
  • the samples 4 are hatched in these and the following figures.
  • FIGS. 3a-b A second embodiment of the microtiter plate 3 according to the invention is shown in FIGS. 3a-b.
  • a layer 9a of the sensor component 9 is provided in the area of the cover 8 , which layer faces the samples 4 and the base plate 5 .
  • the cover 8 is made of glass.
  • the layer 9a is arranged in the area of the base plate 5 .
  • the first elevations 7 are configured in a defined area 11 in such a way that the samples 4 can be positioned in the defined area due to their hydrophilicity or their hydrophobicity or lipophilicity/lipophobicity or ultraphobicity or their surface tension.
  • a defined area 11 can also be arranged on the depressions 6 and/or the cover 8 and/or the second elevations 10 .
  • FIGS. 4a-b A third embodiment of the microtiter plate 3 according to the invention is shown in FIGS. 4a-b.
  • the base plate 5 has indentations 6 which are partially formed by the sensor components 9 .
  • the cover 8 also has second elevations 10 which can be inserted at least partially into the depressions 6 so that the samples 4 can be positioned between the depressions 6 and the second elevations 10 .
  • FIGS. 5a-b A fourth embodiment of the microtiter plate 3 according to the invention is shown in FIGS. 5a-b.
  • the at least one sensor component 9 is arranged both in the area of the cover 8 as a layer 9a and in the area of the base plate as part of the depressions 9 .
  • the samples 4 are in contact with two sensor components 9, as a result of which the sensitivity of the analysis of the samples 4 is increased.
  • the cover 8 is shown as an example with the layer 9a of the sensor component 9 .
  • the crystal body 9b is arranged in the area of the cover 8 and/or in the area of the base plate 5, the at least one defect 9c being arranged in an area of the crystal body 9b facing the samples 4.
  • the microtiter plate 3 is held in a holder 15 .
  • the base plate 5 and/or the cover 8 are transparent at least in the area of the at least one sensor component 9 for the excitation light of the excitation unit 16 and the light emitted by the at least one sensor component 9 .
  • the at least one excitation unit 16 is arranged above the microtiter plate 3 in order to excite the at least one sensor component 9 from above.
  • the detection unit 17 is designed to detect the light emitted by the at least one sensor component 9 and is arranged below the microtiter plate 3, for example.
  • the broken line indicates the path of the excitation light from the excitation unit 16 to the sensor component 9 and the path of the emitted light from the sensor component 9 to the detection unit 17 .
  • an excitation unit 16 and a detection unit 17 are provided, which can be moved relative to the microtiter plate, so that each of the samples 4 can be analyzed individually one after the other.
  • several excitation units 16 and several detection units 17 can also be provided in order to analyze several samples 4 simultaneously.
  • the measuring device 14 has an evaluation unit 18 which uses the light detected by the detection unit 17 to determine at least one chemical and/or physical property of the samples 4 .
  • An alternating frequency source 19 is optionally arranged in the measuring device 14, which is designed to excite the at least one sensor component 9 and/or the samples 4.
  • the measuring device 14 includes an example of an inductor 20, which is designed to induce a preferential polarization of the nuclear spins of the respective sample 4.
  • in the inductor 20 there is a magnetic field device which generates a magnetic field at least in a region of the samples 4 and in a region of the at least one sensor component 9 .

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne une plaque de microtitration (3) pour analyser une pluralité d'échantillons liquides ou pâteux (4), qui se présentent sous la forme de gouttes, au moyen de résonances magnétiques nucléaires des échantillons (4), comprenant - une base (5) avec une pluralité de dépressions (6) qui sont introduites dans la base (5) ou une pluralité de premières élévations (7) pour recevoir les échantillons (4), - un couvercle (8) qui est conçu de telle sorte que les échantillons (4) peuvent être enfermés entre les dépressions (6) ou des premières élévations (7) de la base (5) et le couvercle (8), et - au moins un composant de capteur (9) qui est conçu pour émettre de la lumière influencée par les résonances magnétiques nucléaires des échantillons (4), l'au moins un composant de capteur (9) entrant au moins partiellement en contact avec les échantillons (4) et formant au moins une partie de la base (5) et/ou du couvercle (8), et la base (5) et/ou le couvercle (8) étant transparents à la lumière d'excitation et à la lumière émise au moins dans la région de l'au moins un composant de capteur (9).
PCT/EP2022/070608 2021-08-11 2022-07-22 Plaque de microtitration WO2023016776A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102021120976.5 2021-08-11
DE102021120976.5A DE102021120976A1 (de) 2021-08-11 2021-08-11 Mikrotiterplatte

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WO2023016776A1 true WO2023016776A1 (fr) 2023-02-16

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WO2007047149A2 (fr) * 2005-10-12 2007-04-26 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Porte-echantillon multi-puits en correspondance avec la susceptibilite, pour le criblage a rendement eleve par analyse magnetique
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EP3904897A1 (fr) * 2020-04-29 2021-11-03 Technische Universität München Détection magnétique parallélisée d'échantillons à l'aide de systèmes de rotation à l'état solide

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CA2318881A1 (fr) 1998-02-04 1999-08-12 Tina Garyantes Puits virtuels destines a etre utilises dans des criblages a haut rendement
DE102017205099A1 (de) 2017-03-27 2018-09-27 Robert Bosch Gmbh Sensorvorrichtung, Sensorvorrichtungseinheit, System und Verfahren zum Erfassen einer Messgröße sowie Verfahren zum Herstellen einer Sensorvorrichtung
DE102018216033A1 (de) 2018-09-20 2020-03-26 Robert Bosch Gmbh Sensorvorrichtung

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Publication number Priority date Publication date Assignee Title
EP0550240B1 (fr) 1991-12-31 1998-08-26 Westinghouse Electric Corporation Standard de fréquence atomique
WO2007047149A2 (fr) * 2005-10-12 2007-04-26 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Porte-echantillon multi-puits en correspondance avec la susceptibilite, pour le criblage a rendement eleve par analyse magnetique
US20110062957A1 (en) * 2009-09-11 2011-03-17 Hewlett-Packard Development Company, L.P. Optically integrated biosensor based on optically detected magnetic resonance
GB2483767A (en) * 2010-09-14 2012-03-21 Element Six Ltd A microfluidic cell with a diamond sensor comprising a quantum spin defect
WO2019027917A1 (fr) * 2017-07-31 2019-02-07 Quantum Diamond Technologies, Inc Procédés et appareil de mesure d'échantillon
EP3904897A1 (fr) * 2020-04-29 2021-11-03 Technische Universität München Détection magnétique parallélisée d'échantillons à l'aide de systèmes de rotation à l'état solide

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