WO2023008985A1 - Composition abrasive comprenant de la poire broyée traitée par voie enzymatique pour dentifrice pour animaux de compagnie et composition de dentifrice pour animaux de compagnie la comprenant - Google Patents

Composition abrasive comprenant de la poire broyée traitée par voie enzymatique pour dentifrice pour animaux de compagnie et composition de dentifrice pour animaux de compagnie la comprenant Download PDF

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WO2023008985A1
WO2023008985A1 PCT/KR2022/095080 KR2022095080W WO2023008985A1 WO 2023008985 A1 WO2023008985 A1 WO 2023008985A1 KR 2022095080 W KR2022095080 W KR 2022095080W WO 2023008985 A1 WO2023008985 A1 WO 2023008985A1
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toothpaste
companion animals
composition
pear
abrasive composition
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PCT/KR2022/095080
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English (en)
Korean (ko)
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남승희
강창우
윤선진
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전남대학교 산학협력단
주식회사 브리지테일
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Publication of WO2023008985A1 publication Critical patent/WO2023008985A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses

Definitions

  • the present invention relates to a toothpaste abrasive composition for companion animals containing a pear enzyme-treated material and a toothpaste composition for companion animals containing the same, and more particularly, to a toothpaste abrasive material for companion animals containing a pear enzyme-treated material containing stone cells. It relates to a composition and a toothpaste composition for companion animals comprising the same.
  • Stone cells are also called reinforcing cells or sclerenchyma cells, and have shorter, thicker, irregular, and very hard secondary cell walls than fibrous cells. Stone cells are found in fruit tissues, especially in the pulp of the genus Pyrus. Stone cells are a type of lignification in which lignin is deposited on cell walls in tissues, and it is known that they serve as a framework for fixing cell walls so that they do not change in shape by strengthening cell walls.
  • the main component of stone cells in pear fruits was studied by Ranadive and Haard in Western pears a long time ago. It is known to contain 18 to 35% of lignin, which is not present in the primary cell wall. In addition, the main units of lignin are vanillin and syringaldehyde.
  • toothpaste for companion animals is a kind of tooth cleaner for preventing or removing food debris, bacterial film, plaque, calculus, etc. attached to teeth, and may contain ingredients that prevent tooth decay or bad breath by delivering ingredients effective in preventing tooth decay.
  • Toothpaste includes a liquid type with low viscosity, a paste type with high viscosity, and a powder type, but the paste type is mainly used.
  • the components of pet toothpaste are an abrasive composition that physically removes debris attached to animal teeth, a surfactant that helps effectively remove debris that is firmly attached to animal teeth, and water that has an appropriate viscosity and can maintain its shape.
  • An excipient that helps to prevent hardening and a moisturizer that prevents hardening are the main components, and an abrasive composition such as a flavoring agent, a sweetener, a preservative, a colorant, etc. may be included so that there is no objection when brushing animals.
  • abrasive compositions include monocalcium hydrogen phosphate, precipitated calcium carbonate, silica, and insoluble sodium metaphosphate. These abrasive compositions have a problem in that tooth enamel can be worn because their hardness is higher than that of apatite carbonate, a component of tooth enamel. .
  • the addition amount should be 40% or more in weight ratio and the brushing time should be increased.
  • toothpaste compositions containing natural products have been developed.
  • Conventionally developed oral compositions such as toothpaste have been disclosed that include plant extracts such as green tea extract, moth extract, horsetail extract, and myrrh extract in walnut shell powder, which are effective in removing plaque and preventing gum disease.
  • plant extracts such as green tea extract, moth extract, horsetail extract, and myrrh extract in walnut shell powder
  • the surface is very rough, so the enamel of teeth can be worn, and since most of the walnut shell powder is imported, there is a problem in that expensive costs are required when used for polishing purposes.
  • a toothpaste composition comprising a vegetable abrasive composition obtained by freeze-drying a component containing fibers of plant leaves, stems, and fruit peels and then pulverizing the fiber powder with a starch solution or polymer and coating is disclosed. Since the polishing effect cannot be greatly improved with the same vegetable abrasive composition alone, there is a problem in that an abrasive composition composed of chemical components such as silica, potassium carbonate, sodium bicarbonate, calcium monohydrogen phosphate, and alumina must be included.
  • the object of the present invention is that the effects such as proper polishing of animal teeth, prevention and removal of plaque and calculus, and inhibition of gingivitis are remarkably superior to conventional commercial animal toothpaste or toothpaste simply introducing pear peel components, and brushing teeth by using natural materials It is an object of the present invention to provide a toothpaste abrasive composition for companion animals containing an enzyme-treated pear shell containing stone cells harmless to a living body even when companion animals such as dogs or cats consume toothpaste components, and a toothpaste composition for companion animals containing the same.
  • Another object of the present invention is to select the abrasive composition components using pear shells by particle size without using complicated processes or harmful chemicals, and only by performing alcohol treatment and enzyme treatment to improve the polishing effect of animal teeth, gingivitis ,
  • a method for producing a toothpaste abrasive composition for companion animals comprising an enzyme-treated product of pear shell containing stone cells that is significantly superior to conventional commercial animal toothpaste or toothpaste simply introducing pear shell components in plaque and calculus inhibitory effect, and a companion animal composition comprising the same It is to provide a method for preparing a toothpaste composition for animals.
  • step (a) the heat treatment may be performed at 100 to 140 °C.
  • the alcohol may be a lower alcohol having 1 to 4 carbon atoms.
  • the alcohol may be an aqueous solution of ethanol at a concentration of 40 to 50%.
  • step (d) the Aspergillus niger-derived rapidase may be added in an amount of 0.01 to 0.15 wt% of solids.
  • step (d) the Aspergillus niger derived rapidase is cellulase, hemicellulase, beta-glucanase and pectinase ( pectinase).
  • step (d) the enzymatic treatment may be performed at 35 to 40° C. for 8 to 20 hours.
  • step (d) the hot air drying may be performed at 50 to 70° C. for 10 to 20 hours.
  • a toothpaste abrasive composition for companion animals prepared according to the manufacturing method of the abrasive composition for animal toothpaste is provided.
  • the toothpaste abrasive composition for companion animals may have a total dietary fiber content of 75 to 85% (w/v).
  • the toothpaste abrasive composition for companion animals may have an insoluble dietary fiber content of 65 to 80% (w/v).
  • the stone cell content may be 85 to 95% based on the total weight of the insoluble dietary fiber.
  • the toothpaste abrasive composition for companion animals may have a water absorption capacity of 1.2 to 2 ml/g based on the passage of 1 to 24 hours.
  • a toothpaste composition for companion animals including the toothpaste abrasive composition for companion animals prepared according to the manufacturing method of the toothpaste abrasive composition for companion animals.
  • the toothpaste composition for companion animals may have a moisture content of 25 to 35%.
  • the toothpaste composition for companion animals may include 3 to 15 wt% of the toothpaste abrasive composition for companion animals based on the total weight.
  • the toothpaste composition for companion animals may be used to remove or inhibit gingivitis, plaque or calculus.
  • the toothpaste abrasive composition for companion animals comprising the enzyme-treated pear shell containing stone cells of the present invention and the toothpaste composition for companion animals containing the same are suitable for polishing animal teeth, preventing and removing plaque and calculus, suppressing gingivitis and
  • the same effect is significantly superior to conventional commercial animal toothpaste or toothpaste that simply introduces pear pear components, and by using natural ingredients, there is an effect that is harmless to the body even if companion animals such as dogs and cats ingest toothpaste components when brushing teeth.
  • the manufacturing method of a toothpaste abrasive composition for companion animals containing an enzymatically treated product of pears containing stone cells and the manufacturing method of a toothpaste composition for companion animals including the same of the present invention are complex processes or harmful Simple introduction of conventional commercial animal toothpaste or pear-shell components to prevent polishing of animal teeth, gingivitis, plaque, and calculus, without using chemical products, by sorting the pear material by particle size, and by performing alcohol treatment and enzyme treatment. Significantly better than toothpaste.
  • Figure 3 is a standard curve according to the dyed photograph and color difference value according to the stone cell concentration for examining stone cell purity in Experimental Example 1.
  • GI gingival index
  • PI plaque index
  • CI calculus index
  • Figure 9 is a gingival index (GI), plaque index (PI), calculus index in the teeth of the experimental beagle treated with the commercial animal toothpaste (containing 5% walnut shell) of Comparative Example 3 according to Experimental Example 5 and the toothpaste of Example 3. It is the result of measuring (CI).
  • GI gingival index
  • PI plaque index
  • CI calculus index
  • Example 10 is a photograph showing the dental condition of a beagle after 4 weeks of brushing using the non-treatment according to Experimental Example 5, the commercial animal toothpaste containing walnut shells of Comparative Example 3, and the functional toothpaste of Example 3.
  • the pear pulverized liquid is heat-treated, filtered to remove pear juice, and the by-product is dried to obtain pear pear powder (step a).
  • the heat treatment is preferably performed at 100 to 140 °C, more preferably at 110 to 130 °C, and even more preferably at 115 to 125 °C.
  • the filtration is preferably performed 2 to 3 times using a non-woven fabric to remove pear juice.
  • the pear powder is pulverized and then passed through a 30 mesh mesh and screened to a size that does not pass through a 60 mesh mesh to obtain a sorted pear shell powder (step b).
  • the selected pear powder is preferably selected to a size that passes through a 30 mesh mesh and does not pass through a 60 mesh mesh, more preferably a size that passes through a 30 mesh mesh and does not pass through a 50 mesh mesh, even more preferably Preferably, it can be used by selecting a size that passes through a 30 mesh network and does not pass through a 50 mesh network. In the case of selecting the size of such particles, the polishing performance can be improved by increasing the density of stone cells.
  • the particle size of the pear component may cause a foreign body feeling or damage the teeth, and if it exceeds 60 mesh, the particle size is too small and the abrasive performance for pet toothpaste may deteriorate.
  • the mesh unit was based on KS A 5101 (a unit representing particle size by a standard sieve), and the mesh and micrometer ( ⁇ m) unit conversion table according to this is shown in Table 1 below.
  • step c the selected pear powder is reacted with alcohol to obtain a precipitate.
  • the alcohol is preferably a lower alcohol having 1 to 4 carbon atoms, more preferably a 40 to 50% aqueous ethanol solution, and even more preferably a 43 to 47% concentration It may be an aqueous solution of ethanol.
  • the reaction with the alcohol is preferably carried out while stirring at a speed of 100 to 140 rpm at 20 to 30 ° C., and more preferably reacted while stirring at a speed of 110 to 130 rpm at 23 to 27 ° C. to accelerate the reaction.
  • step d After enzymatically treating the precipitate with Aspergillus niger-derived rapidase, the supernatant is removed from the enzymatically treated product and the precipitate is dried with hot air (step d).
  • the Aspergillus niger derived rapidase is a complex containing cellulase, hemicellulase, ⁇ -glucanase and pectinase. is an enzyme
  • the Aspergillus niger-derived rapidase is preferably added in an amount of 0.01 to 0.15 wt% of solids, more preferably 0.05 to 0.13 wt%, and still more preferably 0.08 to 0.12 wt%. It can be added in wt% amount.
  • sodium acetate sodium phosphate, 2-ethanesulfonic acid (PIPES), water, etc.
  • PPES 2-ethanesulfonic acid
  • sodium acetate may be preferably used.
  • the enzymatic treatment is preferably performed at 35 to 40° C. for 10 to 20 hours.
  • the abrasive composition which has the highest enzyme activity and is an enzyme-treated product, is used in toothpaste for companion animals, the abrasive effect, gingivitis, plaque, and calculus inhibitory effect can be increased.
  • the obtained enzyme-treated material is centrifuged to obtain a precipitate, which can be used as a functional abrasive composition of toothpaste for companion animals.
  • the hot air drying is preferably performed at 50 to 70°C for 10 to 20 hours, more preferably at 55 to 65°C for 13 to 18 hours.
  • the present invention provides a toothpaste abrasive composition for companion animals prepared according to the manufacturing method of the toothpaste abrasive composition for companion animals.
  • the toothpaste abrasive composition for companion animals may have a total dietary fiber content of 75 to 85% (w/v).
  • total dietary fiber means the content including insoluble dietary fiber and soluble dietary fiber.
  • the toothpaste abrasive composition for companion animals may have an insoluble dietary fiber content of 65 to 80% (w/v), preferably 70 to 75% (w/v).
  • the water-soluble dietary fiber content is the total dietary fiber content minus the insoluble dietary fiber content, and since water-soluble dietary fiber has high water absorption, it may reduce formulation stability when used as a toothpaste component, so a formulation with a low water-soluble dietary fiber content It can be advantageous to improve stability.
  • the content of stone cells in the total amount of insoluble dietary fiber may be 85 to 95%, preferably 90 to 93%.
  • stone cells can be defined as components containing lignocellulose and ligne. When the stone cell content is within the above range, polishing performance can be remarkably improved when used as an abrasive composition of toothpaste for companion animals.
  • the high stone cell content in the total amount of insoluble dietary fiber in the toothpaste abrasive composition for companion animals of the present invention is because cellulose, hemicellulose, etc. other than stone cells are removed by the enzyme treatment.
  • the toothpaste abrasive composition for companion animals may have a water absorption capacity of 1.2 to 2 ml/g, preferably 1.4 to 1.6 ml/g, based on the lapse of 1 to 24 hours. This low water absorption capacity seems to be due to the high purity of stone cells, and there is an effect of preventing formulation stability deterioration due to water absorption during toothpaste manufacturing.
  • the present invention provides a toothpaste composition for companion animals comprising the toothpaste abrasive composition for companion animals.
  • the toothpaste composition for companion animals preferably has a moisture content of 25 to 35%, more preferably 27 to 30%.
  • the toothpaste composition for companion animals preferably contains 3 to 15 wt% of the toothpaste abrasive composition for companion animals, more preferably 5 to 10 wt%, based on the total weight. If it is included in less than 3wt%, the abrasive effect, gingivitis, plaque and calculus inhibitory effect may be reduced, and if it exceeds 15wt%, the tooth surface may be damaged or the gums may be irritated or damaged.
  • the toothpaste composition for companion animals may be selected from cream, powder, paste, gel and liquid types.
  • the toothpaste composition for companion animals may be used for removing or inhibiting gingivitis, plaque or calculus.
  • the toothpaste composition for companion animals of the present invention may include a detergent together with the toothpaste abrasive composition for companion animals.
  • Conventional surfactants may be used as the cleaning agent. These detergents prevent foreign substances separated from the tooth surface during brushing from adhering to the tooth surface again, and function to ensure that they are completely discharged from the mouth during brushing. These detergents include alkyl sulfates, olefin sulfonates, monoglyceride sulfonates, etc.
  • Anionic surfactants such as polyoxyethylene sorbitan fatty acid ester, polyoxyethylene castor oil, glycerin fatty acid ester, nonionic surfactants such as alkylolamide, and amphoteric surfactants such as Pluronic F-108. can
  • an excipient may be used to increase the weight or form a paste, etc.
  • an excipient means an orally acceptable carrier.
  • the toothpaste composition may further include xylitol, a fluorine compound, or a caries preventing component composed of a mixture of two or more thereof in an amount of 0.1 to 5% by weight based on the total weight of the toothpaste composition.
  • the xylitol is obtained from a natural substance such as a birch tree extract, and is known to be effective in preventing tooth decay by suppressing the propagation of bacteria while having a sweet taste but not containing sugar.
  • the xylitol can also be used as a sweetener.
  • Fluorine compounds have also been known to be effective in preventing tooth decay for a long time, and are known to those skilled in the art to easily purchase and use them.
  • wetting agents in the toothpaste composition for companion animals of the present invention, other wetting agents, binders, foaming agents, sweeteners, flavoring agents, coloring agents, or auxiliary components consisting of a mixture of two or more of them are present in an amount of 0.1 to 50% by weight based on the total weight of the toothpaste composition. More may be included.
  • These wetting agents, foaming agents, sweetening agents, flavoring agents, and coloring agents are also things that can be understood as known enough to be easily purchased and used by those skilled in the art of toothpaste manufacturing.
  • auxiliary components may be included in less than 0.1% by weight based on the total weight of the toothpaste composition, there may be a problem in that it is difficult to form an appropriate toothpaste by these auxiliary components, and conversely, even if it exceeds 50% by weight, other There may be a problem that causes a lack of content of components.
  • a humectant is an essential base component for making toothpaste formulations, and prevents toothpaste from drying and solidifying when exposed to air, provides gloss to the surface of toothpaste, and also serves to give a sweetening effect when brushing teeth depending on the type.
  • the binder serves to prevent separation between the solid powder component and the liquid component. Any type of water-soluble polymer can be used, and commonly used ingredients include sodium carboxymethylcellulose synthesized from cellulose of trees, carrageenan extracted from seaweed, and xanthan gum obtained from metabolism of microorganisms. .
  • the foaming agent enhances the feeling of use of the product, helps in cleaning, and quickly disperses and penetrates other active ingredients, and reduces interfacial tension to easily remove foreign substances in the oral cavity.
  • sodium lauryl sulfate which is an anionic surfactant, polyoxyethylene-polyoxypropylene copolymer (poloxamer), polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, and the like may be used.
  • spices, sweeteners, colorings, etc. may be used to improve the feeling of use.
  • spices used for this purpose it is common for animals to consume even a small amount when brushing their teeth, so it is essential to use edible spices.
  • Saccharin sodium and stevioside are used as the sweetening agent, and food coloring is preferably used as the colorant.
  • the present invention provides a method for manufacturing a toothpaste composition for companion animals, including a method for manufacturing a toothpaste abrasive composition for companion animals.
  • the selected pear powder prepared according to Preparation Example 2 was treated with ethanol and enzyme.
  • Enzyme treatment is performed by adding 0.1% by weight of cellulase to the Aspergillus niger-derived Rapidase, and then using sodium acetate buffer (0.01M, pH 5.0). ) was added in 450 ml and reacted for 15 hours at a speed of 80 rpm at 37 ° C. After centrifugation, the supernatant was completely removed and dried at 60 ° C. for 15 hours.
  • Example 1 Preparation of functional toothpaste for companion animals containing pear shell powder
  • a functional toothpaste containing the pear shell powder of Preparation Example 1 was prepared with the composition shown in Table 2 below, and the abrasive composition component included 5 wt% of the pear shell powder instead of 5 wt% of the cellulose gum of the commercial animal toothpaste.
  • Example 2 Preparation of functional toothpaste for companion animals containing selected pear powder
  • a functional toothpaste was prepared under the same conditions as in Example 1, except that the selected pear powder of Preparation Example 2 was included instead of the pear powder of Preparation Example 1.
  • Example 3 Preparation of functional toothpaste for companion animals containing enzymatically treated animal toothpaste abrasives
  • a functional toothpaste was prepared under the same conditions as in Example 1, except that the enzyme-treated animal toothpaste abrasive of Preparation Example 3 was included instead of the pear shell powder of Preparation Example 1.
  • Comparative Example 1 Commercial Cellulose Gum Animal Toothpaste
  • a commercial animal toothpaste was prepared, and one containing cellulose gum as an abrasive composition component was prepared.
  • a commercial animal toothpaste was prepared, and a product with high abrasive performance was prepared by containing 10 wt% of cellulose gum instead of 5 wt% as an abrasive composition component.
  • a commercial animal toothpaste was prepared, and one containing walnut shell powder instead of cellulose gum as an abrasive composition component was prepared.
  • Ingredients Content (wt%) Abrasive Composition Ingredients 5 to 10 Water dose Glycerin 9-11 PEG-40 Hydrogenated Castor Oil 1.23 Hydrolyzed Collagen 12.5 Fragrance dose Enzyme-modified steviol glycosides 8 to 12 Citric Acid 0.09 to 0.13 Potassium Sorbate 0.8 ⁇ 1.2 Zinc citrate 0.004 ⁇ 0.01 Sodium Benzoate 0.004 ⁇ 0.01 Menthol dose Butylene Glycol 1 ⁇ 2 Enzymatically Modified Rutin 4-7 entire 100
  • Dietary fiber was analyzed according to the method of Prosky et al. (1988).
  • IDF insoluble dietary fiber
  • 1 g of the sample was turbid in 50 ml of phosphate buffer (pH 6.0), heat-resistant ⁇ -amylase at 95 ° C, and protease at 60 ° C. and amyloglucosidase were sequentially treated with enzymes.
  • the filtered residue was washed with 75% ethanol, 95% ethanol and acetone, and dried for 12 hours, and the value was measured excluding protein and ash content.
  • the total dietary fiber (TDF) content was calculated as the sum of the insoluble and soluble dietary fiber content of the residue.
  • Stone cells can be defined as those containing lignocellulose and lignin, and a staining method that can be distinguished from insoluble dietary fibers composed of simple cellulose or hemicellulose is required.
  • Wiesner staining solution was composed of 2% phloroglucinol, 20% ethanol, and 20% hydrochloric acid (HCl), and was left for about 2 hours after preparation.
  • the redness value (a-value) was measured using a colorimeter, and the stone cell content contained in the insoluble dietary fiber powder was quantified using a standard curve to which the concentration and redness value of stone cells were applied. .
  • the standard curve to which the dyed photo and the redness value according to the stone cell concentration are applied is shown in FIG. 3, and the color difference value for each component content is shown in Table 3 below.
  • the stone cell concentration can be measured according to the a value.
  • the enzyme-treated animal toothpaste abrasive of Preparation Example 3 had a high stone cell purity of 92%, and the pear shell powder of Preparation Example 1 had a relatively low stone cell content and dietary fiber accounted for most of it at 75%.
  • the water absorption capacity that causes formulation instability was calculated according to Equation 1 below.
  • V wet represents the volume of the hydrated sample
  • V dry represents the volume of the dry sample
  • W wet represents the mass of the dry sample
  • the pear shell powder of Preparation Example 1 having a relatively high water-soluble dietary fiber ratio and a low stone cell content had a very high water absorption capacity of 19 ml / g, and the selected pear shell powder of Preparation Example 2 had 3.2 ml / g
  • Preparation Example 1 The enzymatically treated abrasive for animal toothpaste showed very low water absorption even after 24 hours with 1.5 ml/g of germ cell B. As can be seen in the microscopic image, it seems to be due to the high purity of the stone cells that are red. If the water absorption capacity is low, it may have high formulation stability.
  • the functional toothpaste for companion animals of Comparative Example 1 showed a sugar content of about 15 ° brix, similar to Examples 2 and 3, and the functional toothpaste for pets of Example 1 had a sugar content of 17.7 ° brix, which was relatively low. It was high.
  • the L value which is the brightness in the chromaticity of the sample, was 4.34 for the toothpaste of Comparative Example 1, 29.32 for the toothpaste of Example 2, 32.91 for the toothpaste of Example 3, and 19.38 for the toothpaste of Example 1.
  • the toothpaste of Example 3 was the highest.
  • the redness a value was 0.03 for the commercial animal toothpaste of Comparative Example 1, 10.32 for the toothpaste of Example 2, 12.92 for the toothpaste of Example 3, and 4.30 for the toothpaste of Example 1 1. toothpaste was the highest.
  • the yellowness b value was -1.96 for the commercial animal toothpaste of Comparative Example 1, 19.02 for the toothpaste of Example 2, 21.77 for the toothpaste of Example 3, and 9.60 for Example 1.
  • Gumminess was measured using a texture meter (XforceP, Zwick Roell Co., Germany), and the measurement condition was to attach a probe with a diameter of 15 mm at a start position speed of 5 mm/s, once The compression ratio was set to 70% at the time of measurement and 20% at the time of measurement when measuring twice.
  • Moisture content (%) was measured according to the loss on drying test method. 1 g of sample was precisely weighed, dried at 105 ° C. for 4 hours, left in a desiccator to cool, and then calculated according to Equation 3 below.
  • Moisture content (%) ⁇ 1-weight after drying (g)/weight of sample (g) ⁇ ⁇ 100
  • Density was calculated according to Equation 4 below by putting the sample into a container of known capacity and measuring its weight.
  • Density (g/mL) sample weight (g)/container (mL)
  • Swelling Capacity is measured by mixing 1 g of sample with 20 ml of water (1:20, w/v) and then leaving it at 4 ° C. It was measured according to Equation 1 above.
  • the adhesiveness of the toothpaste of Example 1 containing a lot of dietary fiber was the highest at 397, and the toothpaste of Example 3, which had a high content of stone cells, was similar in adhesiveness to the commercial animal toothpaste of Comparative Example 1, resulting in marketability. appeared to be high.
  • the toothpaste of Example 1 having a high dietary fiber content was 58.3%, and the remaining toothpastes were 28 to 30%. It showed about 28% lower content than the toothpaste of Example 1. Density showed little difference between samples, and the water absorption capacity that causes the layer separation of the toothpaste and the expansion of the formulation was the lowest for the toothpaste of Example 3, which was about 1.4 even after 24 hours, similar to the commercial animal toothpaste 1.2 of Comparative Example 1. value was indicated. That is, the toothpaste of Example 3 exhibiting low adhesiveness, moisture content, and water absorption capacity as a whole exhibited a high formulation stability similar to that of the commercial animal toothpaste of Comparative Example 1.
  • the polishing effect was 14.0% for the commercial animal toothpaste of Comparative Example 2, 20.1% for the toothpaste of Example 1, and 31.6 or 42.7% for the toothpaste of Examples 2 and 3, respectively. That is, the toothpaste of the present invention containing high-purity stone cells showed a higher polishing effect than the commercial animal toothpaste of Comparative Example 2 with a high abrasive composition content or the toothpaste of Example 1 with a relatively low content of stone cells. .
  • the remaining calculus content was qualitatively evaluated through the staining method.
  • the irradiated area Measurements were taken at 3 sites per tooth (distal buccal, mesibuccal, buccal) of the left and right maxillary third incisors, maxillary and mandibular canines, maxillary and mandibular 3rd and 4th premolars, and maxillary and mandibular first molars.
  • gingivitis, plaque, and calculus evaluation progress evaluation was performed, and gingivitis, plaque and calculus removal (scale & polish) were performed under anesthesia, and fecal evaluation was performed twice a week.
  • Gingivitis, plaque, and calculus were evaluated at weeks 2 and 4 and fecal evaluation twice a week. Brushing is performed once/day for 4 weeks, and the clinical indices evaluated at this time are the Gingival Index (GI), Plaque Index (PI), and Calculus Index (CI), and the evaluation criteria are as follows. The data obtained in all experiments were verified for significance with the Multiple Mann-Whitney test, and these values were expressed as mean ⁇ SD values, and significance was determined as significant when the p value was less than 0.05 did
  • the gingival inflammation status was scored using the Silness and Loe Cingival Index in the following way.
  • Gingival Inflammation Index sum of total scores / total number of gums examined
  • Verdict 0.1-1.0 (mild), 1.1-2.0 (moderate), 2.1-3.0 (severe)
  • Supragingival plaque coverage and plaque thickness were evaluated using the Turesky-Quigley-Hein Plaque index. Each tooth surface was stained with a red colorant (Erythrosin, sultan, USA) and scored in the following way. Scores are according to Table 7 below.
  • Plaque index (PI) sum of (coverage * thickness) / total number of tooth surfaces examined
  • Verdict 0.1-1.0 (mild), 1.1-2.0 (moderate), 2.1-3.0 (severe)
  • the Waltham Faeces Scoring system evaluation criteria which are stool evaluation criteria, are shown in FIG. 7 .
  • the results of measuring the gingival index (GI), plaque index (PI), and calculus index (CI) of the untreated control group without brushing are shown in FIG. 8 .
  • the gingival index (GI) was measured as 0.37 at the 0th week, 1.08 at the 2nd week, and 1.23 at the 4th week.
  • Plaque index (PI) was measured as 0 at week 0, 2.96 at week 2, and 5.53 at week 4.
  • the calculus index (CI) was measured as 0 at week 0, 1.94 at week 2, and 3.00 at week 4.
  • the gingival inflammation index, plaque index, and calculus index significantly increased in the 2nd and 4th weeks in the untreated control group.
  • the gingival index (GI) at 2 weeks was measured as 1.08 in the control group, 0.65 in the group brushed using the toothpaste of Example 3, and 0.83 in the group using the commercial animal toothpaste containing walnut shells.
  • the 4th week untreated control group was measured at 1.23
  • the group brushing using the toothpaste of Example 3 was 0.61
  • the group using the commercial animal toothpaste containing walnut shells of Comparative Example 3 was measured at 1.02.
  • the commercial animal toothpaste containing walnut shells of Comparative Example 3 decreased the gingivitis level only at the 2nd week, and the gingivitis level of the toothpaste of Example 3 significantly decreased significantly at the 2nd and 4th weeks.
  • plaque index (PI) was measured at 2.96 in the untreated control group at 2 weeks, 3.70 in the group brushed using the toothpaste of Example 3, and 3.36 in the group using the commercial animal toothpaste containing walnut shells of Comparative Example 3. has been measured
  • the 4th week untreated control group was measured at 5.53
  • the group using the toothpaste of Example 3 was measured at 4.12
  • the group using the commercial animal toothpaste containing walnut shells of Comparative Example 3 was measured at 4.67. That is, the group using the toothpaste of Example 3 tended to have a slightly lower plaque index (PI) at 4 weeks compared to the group using the commercial animal toothpaste containing walnut shells of Comparative Example 3.
  • Calculus index (CI) was measured at 1.94 in the untreated control group at 2 weeks, 0.80 in the group brushed using the toothpaste of Example 3, and 1.00 in the group using commercial animal toothpaste containing walnut shells in Comparative Example 3. .
  • the untreated control group at 4 weeks was measured at 3.00
  • the calculus index of the group brushed using the toothpaste of Example 3 was 1.77
  • the group using the commercial animal toothpaste containing walnut shells of Comparative Example 3 was measured at 2.53. That is, compared to the untreated control group, the group using the toothpaste of Example 3 showed a significant decrease in calculus index (CI) in both the 2nd and 4th weeks.
  • the gingivitis level was reduced by 23% or 39.9%
  • the plaque index was reduced by 15.6%, 25.5%, and calculus.
  • the index was significantly increased by 15.7% and 41.0%, respectively, and compared to the walnut shell-containing toothpaste of Comparative Example 3, the toothpaste of Example 3 of the present invention was found to be particularly effective in inhibiting gingivitis and calculus.
  • FIG. 10 a photograph showing the dental condition of the beagle after 4 weeks of brushing using the untreated, commercial animal toothpaste containing walnut shells of Comparative Example 3 and the functional toothpaste of Example 3 is shown in FIG. 10 .
  • the functional toothpaste of Example 3 of the present invention was applied, the condition of the teeth seemed as clean as possible.
  • the toothpaste abrasive composition for companion animals comprising the enzyme-treated pear shell containing stone cells of the present invention and the toothpaste composition for companion animals containing the same are suitable for polishing animal teeth, preventing and removing plaque and calculus, suppressing gingivitis and
  • the same effect is significantly superior to conventional commercial animal toothpaste or toothpaste that simply introduces pear pear components, and by using natural ingredients, there is an effect that is harmless to the body even if companion animals such as dogs and cats ingest toothpaste components when brushing teeth.
  • the manufacturing method of a toothpaste abrasive composition for companion animals containing an enzymatically treated product of pears containing stone cells and the manufacturing method of a toothpaste composition for companion animals including the same of the present invention are complex processes or harmful Simple introduction of conventional commercial animal toothpaste or pear-shell components to prevent polishing of animal teeth, gingivitis, plaque, and calculus, without using chemical products, by sorting the pear material by particle size, and by performing alcohol treatment and enzyme treatment. Significantly better than toothpaste.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Cosmetics (AREA)

Abstract

La présente invention concerne un procédé de préparation d'une composition abrasive pour dentifrice pour animaux de compagnie et une composition de dentifrice, ainsi préparée, pour animaux de compagnie, le procédé comprenant les étapes consistant à : (a) traiter thermiquement la poire broyée, filtrer le jus de poire et sécher le résidu pour obtenir une poudre de poire broyée ; (b) pulvériser la poudre de poire broyée et tamiser la poudre pulvérisée par taille pour sélectionner une poudre de poire broyée qui peut passer à travers une maille de 30 mais ne peut pas traverser une maille de 60 ; (c) faire réagir la poudre de poire broyée sélectionnée avec un alcool pour obtenir un précipité ; et (d) traiter par voie enzymatique le précipité avec une rapidase dérivée d'Aspergillus niger, retirer le surnageant de la matière traitée par voie enzymatique et sécher le précipité avec de l'air chaud. Ainsi, la composition de dentifrice présente des effets remarquablement meilleurs que les dentifrices ou dentifrices pour animaux de compagnie classiques que l'on trouve dans le commerce ayant comme ingrédient la poire broyée simplement introduit dans ceux-ci, tels que l'abrasion correcte des dents animales, la prévention et le retrait de plaques et de tartre dentaires et la suppression de gingivite En utilisant des matériaux naturels, la composition du dentifrice est inoffensive pour les animaux de compagnie tels que les chiens ou les chats même lorsqu'ils ingèrent les ingrédients de dentifrice pendant le brossage des dents.
PCT/KR2022/095080 2021-07-28 2022-04-08 Composition abrasive comprenant de la poire broyée traitée par voie enzymatique pour dentifrice pour animaux de compagnie et composition de dentifrice pour animaux de compagnie la comprenant WO2023008985A1 (fr)

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KR1020210099349A KR102376424B1 (ko) 2021-07-28 2021-07-28 배박 효소 처리물을 포함하는 반려동물용 치약 연마재 조성물 및 이를 포함하는 반려동물용 치약 조성물
KR10-2021-0099349 2021-07-28

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KR102376424B1 (ko) * 2021-07-28 2022-03-21 전남대학교산학협력단 배박 효소 처리물을 포함하는 반려동물용 치약 연마재 조성물 및 이를 포함하는 반려동물용 치약 조성물

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KR20140147965A (ko) * 2013-06-20 2014-12-31 전라남도 배 식이섬유를 포함하는 변비 또는 비만의 예방 또는 개선용 조성물
KR20140147964A (ko) * 2013-06-20 2014-12-31 전라남도 배를 이용한 기능성 올리고당의 제조방법
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