WO2023005127A1

WO2023005127A1 - Algae inhibitor and preparation method therefor

Info

Publication number: WO2023005127A1
Application number: PCT/CN2021/141047
Authority: WO
Inventors: 高静思; 巫俊铭; 陈菊; 廖萍; 罗红梅; 陈海珊; 陈佳旭; 吴玉婵; 何钟亿; 谢志旋; 王卫华; 罗乔; 赖敏
Original assignee: 深圳职业技术学院; 吉安职业技术学院
Priority date: 2021-07-30
Filing date: 2021-12-24
Publication date: 2023-02-02
Also published as: CN113603195A

Abstract

An algae inhibitor and a preparation method therefor. The method comprises: providing a carrier; adding plant medicinal powder and a filler into a solvent to obtain a mixed solution; and spray-coating the mixed solution on the surface of the carrier and then drying, thus obtaining the algae inhibitor. The preparation method involves a simple process, the raw material ingredients are easy to obtain, and no toxic or harmful solvents are used. The prepared algae inhibitor can effectively inhibit algae propagation, is environmentally friendly, and does not cause secondary pollution.

Description

A kind of algae inhibitor and preparation method thereof

technical field

The invention relates to the technical field of algae control, in particular to an algae inhibitor and a preparation method thereof.

Background technique

The eutrophication of the water body causes a large number of algae to multiply in the water, which causes the pressure of the raw water treatment of the water plant and brings risks to the safe water supply of the city.

At present, the control of algae is mainly divided into two categories. The first category is to solve the problem of eutrophication as the fundamental purpose, to control the nutrients necessary for the growth of algae as the main way, and to intercept pollution, control sources, and overall restoration as the core means. Systematic reservoir in situ restoration program. This type of technology focuses on "rectifying the source" and is the most fundamental solution to the problem from a long-term perspective. However, it has many influencing factors, takes a long time, and is slow to take effect. Some water bodies with serious endogenous pollution may need to experience ten years of treatment. It will take years or even decades of hard work to completely eliminate the risk of algal blooms. The second category is in-situ short-term algae control technology, which uses various physical, chemical and biological means to inhibit the growth of algae or remove a large number of algae to ensure the safety of water bodies. Among them, the physical methods include water pumping and aeration anti-algae technology, ultraviolet radiation anti-algae technology, etc.; the chemical method is typically represented by adding algae-inhibiting chemicals such as copper sulfate; the biological method mainly adopts fish stocking, microbial breeding and aquatic plant planting and other methods. However, physical methods and traditional biological methods have the disadvantages of long operation time, difficulty and high cost; chemical methods may destroy the ecological balance and cause secondary pollution.

Therefore, how to control algae in a low-cost and environmentally friendly way is an urgent problem to be solved.

Contents of the invention

In view of the above deficiencies in the prior art, the purpose of the present invention is to provide an algae inhibitor and a preparation method thereof, which are used to solve the problems that existing algae control is likely to cause secondary pollution and destroy ecology.

In a first aspect, the present invention provides a method for preparing an algae inhibitor, which includes:

provide a carrier;

Adding plant medicine powder and filler to the solvent to obtain a mixed solution;

After the mixed solution is sprayed on the surface of the carrier and dried, the algae inhibitor is obtained.

Optionally, in the preparation method, wherein, the botanical medicinal material is selected from one or more of crane lily, reed, big weed, water sword leaf, litchi leaf, magnolia and renmianzi.

Optionally, in the preparation method, the carrier is selected from one of zeolite, carbon nanotube and organic framework material.

Optionally, in the preparation method, wherein, after the mixed solution is sprayed on the surface of the carrier and dried, the step of obtaining the algae inhibitor specifically includes:

Atomizing the mixed solution to form particles, and attaching the particles to the surface of the carrier to obtain a semi-finished product;

The semi-finished product is put into drying equipment to dry to obtain the algae inhibitor.

Optionally, in the preparation method, the solvent is water.

Optionally, in the preparation method, the filler is hydroxypropyl starch and/or hypromellose.

Optionally, in the preparation method, the mass ratio of the plant drug powder to the filler is 100:0.1-5.

Optionally, in the preparation method, the particle size of the particles is 50-100 nm.

In the second aspect, an algae inhibitor is prepared by the above-mentioned preparation method.

Optionally, the algae inhibitor, wherein, the algae inhibitor also includes a base material for carrying the algae inhibitor, the base material is provided with several mesh holes, and the algae inhibitor is fixed on the inside the mesh.

Beneficial effects: the embodiment of the present invention provides an algae inhibitor and a preparation method, wherein the preparation method has a simple process, and each raw material component is easy to obtain, and does not involve toxic or harmful solvents. The prepared algae inhibitor can effectively reproduce algae, is environmentally friendly and does not cause secondary pollution.

Description of drawings

Fig. 1 is the curve diagram of the influence of the dosage of the active ingredient Craneus chinensis in the algae inhibitor on the growth of Pseudo-Anabaena;

Fig. 2 is the influence curve figure of the active ingredient white crane lily in the algae inhibitor on the maximum photon yield of pseudoantaba;

Fig. 3 is a graph showing the IR change curve of the maximum photon yield IR of Pseudomonas anabaena at the dosage of 1.02g/L;

Fig. 4 is a histogram of the influence of the dosage of the active ingredient Craneus japonicus in the algae inhibitor on the inhibition rate of the maximum photon yield of Anabaena pseudoantha;

Fig. 5 is the influence curve diagram of the initial slope of the initial slope of the active ingredient Craneus chinensis in the algae inhibitor;

Figure 6 is a graph showing the initial slope IR change curve of Pseudo Anabaena under the dosage of 12g/L;

Fig. 7 is a histogram of the influence of the dosage of the active ingredient Craneus chinensis in the algae inhibitor on the initial slope;

Fig. 8 is a curve diagram of the influence of the dosage of the active ingredient Craneus chinensis in the algae inhibitor on the electron transfer rate of Pseudomonas anabaena;

Fig. 9 is a graph showing the change curve of the inhibition rate of the electron transfer rate of pseudoantha anabaena under the dosage of 1.02g/L;

Fig. 10 is a curve diagram of the influence of the dosage of the active ingredient Craneus japonicus in the algae inhibitor on the half-saturated light intensity;

Fig. 11 is a graph showing the effect of the dosage of active ingredient Craneus chinensis in the algae inhibitor on the spontaneous chlorophyll fluorescence of Pseudomonas anabaena.

Detailed ways

The present invention provides an algae inhibitor and a preparation method thereof. In order to make the purpose, technical scheme and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terminology used herein in the description of the present invention is only for the purpose of describing specific embodiments, and is not intended to limit the present invention.

The embodiment of the present invention provides a preparation method of an algae inhibitor, the method comprising:

S10. Provide a carrier.

Specifically, the carrier refers to the object used to carry the medicine that can inhibit the growth of algae. The carrier can be zeolite (artificial zeolite, natural zeolite), or carbon nanotube, organic framework material, etc. The carrier used For an object with a porous surface, use the pores on the carrier to immobilize the drug. By attaching the medicament to the carrier with a porous surface, the local medicament concentration can be avoided from being too high, affecting the ecology of the water body, and the efficacy of the drug can also be extended appropriately.

S20, adding the herbal medicine powder and the filler into the solvent to obtain a mixed solution, wherein the solvent is water.

Specifically, the plant medicinal material powder refers to a plant that is dried and ground into a fine powder, wherein the leaves or rhizomes of the plant are selected according to different plant types. Wherein, the type of the plant can be floating plants, such as eye lotus, water peanut, water water lotus, Manjianghong, duckweed, purple duckweed, duckweed, Daping, etc.; Flowers, calamus, reeds, lotus, amphibian polygonum, stone calamus, red Polygonum, reed bamboo, calla lily, water chestnuts, rushes, etc.; wet herbaceous plants, such as white crane taro, mushroom grass, anthurium; submerged water plants, such as Foxtail algae, water grass, water shield grass, black algae, water sword leaf, chrysanthemum algae, charophytes, licorice genus, eye genus, hornwort algae, eczema; terrestrial plants, such as barley straw, Litchi, Magnolia, Renmianzi, Ash Willow, etc.

Exemplarily, the Daping can effectively inhibit various cyanobacteria, green algae, golden algae, and red algae; reed can effectively inhibit the water bloom Microcystis flos-aquae, Microcystis aeruginosa, and Chlorella pyrenoidosa The white crane taro can effectively suppress Microcystis aeruginosa; The water sword leaf can effectively suppress Microcystis aeruginosa, Nannochloropsis oculata (Nannochloropsis oculata), Nitzchia palea (Nitzchia palea), Synechococcus elongatus ( Chlorella elongata), Scenedesmus obliquus (Scenedesmus obliquus), filamentous cyanobacteria (Filamentous cyanobacteria); described lychee leaves, Magnolia japonica, Renmianzi can effectively inhibit Microcystis aeruginosa; Haematococcus pluvialis, Anabaena blooms, Anabaena streptococcus, Oscillatoria tenuis.

In this embodiment, the function of the filler is mainly for molding, that is, after the herbal medicine is made into powder, it is sprayed to form mixture particles. Wherein, the filler may be hydroxypropyl starch or hypromellose, or a mixture of hydroxypropyl starch and hypromellose. The ratios of the filler to the powder of the plant medicine are 100:0.5, 100:1, 100:2, 100:3, 100:4, 100:5. The addition of too little filling agent will affect the formation of spray particles, and the addition of too much will affect the drug effect.

S30. After spraying the mixed solution on the surface of the carrier and drying it, the algae inhibitor is obtained.

Specifically, the mixed solution can be added to a high-pressure spray device, and the mixed solution can be formed into fine particles by high-pressure spray, and the particle size of the fine particles can be 50nm to 60nm, 60nm to 70nm, 70nm to 80nm, 80nm to 90nm , 90nm to 100nm. The resulting fine particles are adsorbed in the micropores on the surface of the carrier, such as in the pores on the surface of zeolite. Then baked at low temperature to finally get the inhibitor that can inhibit algae. It is easy to understand that the baking can be done in an oven or in an oven on an assembly line. The baking temperature can be 30 degrees Celsius.

Based on the same inventive concept, an embodiment of the present invention also provides an algae inhibitor, which is prepared by the above-mentioned preparation method.

In this embodiment, the algae inhibitor further includes a base material for carrying the algae inhibitor, the base material is provided with several meshes, and the algae inhibitor is fixed in the meshes. Exemplarily, plastic or foam with mesh holes on the surface can be used, and then the algae inhibitor is embedded in the mesh, and the algae inhibitor can be conveniently dispensed by embedding the algae inhibitor in the mesh. At the same time, the substrate can also be set at a corresponding depth according to the distribution position of the algae in the water body. For example, cyanobacteria are mostly located 6 cm away from the water surface, so the substrate can be set at a position 5 cm away from the water surface. Thereby, the cyanobacteria can be better suppressed.

The algae inhibitor provided by the present invention will be further explained through specific examples below.

Example 1

Take the root of the mature Spathiphyllum Kochii plant and dry it, grind it into powder, weigh hydroxypropyl starch according to the ratio of 100:0.1, add it to the aqueous solution to obtain a mixed solution, and add the obtained mixed solution to a high-pressure spray device In the process, the mixed solution is prepared into tiny particles by spraying, and the tiny particles are adsorbed on the surface of the zeolite, and baked at a low temperature to obtain an algae inhibitor.

Add the prepared algae inhibitor into the experimental box containing Pseudo-Anabaena, and add 0g, 0.04g, 0.08g, 0.17g, 0.25g, 0.34g, 0.68g, 1.02g of Siberian Crane per liter of water The addition amount of taro powder was added, and then the growth curve of Pseudo-Anabaena was drawn, and the results are shown in Figure 1. It can be seen that when the dosage is 1.02g/L, the growth of Pseudo-Anabaena can be significantly inhibited. Among them, the mechanism of inhibition of pseudoantaba is explained as follows:

The maximum light quantum yield, the maximum light quantum yield of algae is measured under saturated pulse conditions after full dark adaptation, reflecting the quantum yield when all PS II reaction centers are in the open state, and is an important indicator for studying the effect of photoinhibition on photosynthesis. Under photoinhibition conditions, a decrease in the maximum photon yield indicated that the algae were stressed.

A certain concentration of white crane taro can stress the photosynthesis of Pseudomonas anabaena, as shown in Figure 2, at the dosage of 1.02g/L, the inhibition rate of the maximum photon yield of Pseudo Anabaena rose to 70% within 2 days or so, followed by some fluctuations, but stabilized at around 75% after the 10d. Combined with Figure 5-3, on the 7th day, the inhibition rate of the experimental group with an dosage of 0.17-0.34g/L was higher than that of the other groups. With the growth of the culture time, the IR of the high dosage group gradually increased until the 15th day , the inhibition rate of the experimental group whose dosage was above 0.25g/L basically reached a close level, exceeding 90%, and the inhibition rate increased with the increase of dosage.

Effective photon yield

The effective photon yield is measured during photosynthesis under light conditions, and represents the efficiency with which excitation energy is captured by the open reaction center, reflecting the degree of photochemical limitation of PS II due to the competition of thermal energy dissipation.

As shown in Figures 3 to 4, the IR of the effective photon yield of 0.08g/L dosage is basically stable at an average level of 30%. With the increase of the dosage, the IR gradually increased, and the IR of the dosage of 1.02g/L stabilized at about 80% after the 9th day, and the effective light quantum of the experimental groups with the dosage of 0.34 and 0.68g/L after the 9th day The changes were basically the same as those in the 1.02g/L experimental group, and there was a slight increase in the 0.25g/L experimental group in the later period. On the 7th day, the inhibition rate of each experimental group was quite different, and the 0.25g/L dosage group was the highest, reaching 85.0%, followed by 0.34 and 0.17g/L, then 0.68 and 0.08g/L, and finally 1.02 and 0.04 g/L. Combined with the change of the maximum photon yield, it can be inferred that at the dosage of 0.25-0.34g/L, the IR of the photon yield of Anabaena pseudoannae on the 7th day is the highest, and the decrease or increase of the dosage will lead to the decline of IR. However, after prolonging the culture time, after the 9th-10th day, the IR of the high-concentration dosage experimental group will continue to rise, but the IR of the 0.25-0.34g/L experimental group will decline to a certain extent. It is speculated that this is mainly due to the high-concentration dosage Quantitative substances remain in the culture system for a longer period of time, and the reason for the longer-lasting inhibitory effect is also achieved.

Fast light curve initial slope

The initial slope of the fast light curve of algae photosynthesis reflects the efficiency of light energy utilization by Anabaena pseudoantha. Figure 5 shows the change of the initial slope of Pseudomonas anabaena under different dosages of SKRE, which is similar to the change trend of the effective photon yield, and the initial slope is also subject to the same inhibition. It can be seen from Figures 6 and 7 that the initial slope IR produced by the dosage of 0.68 and 1.02g/L was the highest during the 1-3d of the culture, and the initial slope IR produced by the dosage of 3-4g/L was the highest on the 4-9d, followed by 0.17, 0.08, 0.68, 1.02 and 0.04g/L, after the 9th day, the initial slope IR increased more regularly with the increase of dosage.

In general, as the culture time prolongs, the IR of the initial slope of the low dosage group (0.04, 0.08g/L) and the high dosage group (0.34-1.02g/L) gradually increases; High and then down. With the increase of dosage, in the early stage (0-3d), the IR of the initial slope first increased and then decreased, and then the regeneration was high; in the middle period (4-9d), the IR of the initial slope first increased and then decreased; 18d), IR increases with the increase of dosage, but the difference is not significant under the dosage of 0.25-1.02g.

electron transfer rate

Figure 8 is the change curve of electron transfer rate (ETR) in photosynthesis of Anabaena pseudoannae at different dosages. The inhibitory effect of ETR is not stable. There was a promotion effect, and with the increase of dosage, there was no obvious change rule in the early and mid-term inhibition rate, and there was no obvious difference in the inhibition rate between the dosage of 0.25-1.02g/L in the later period.

The effect of the dosage of 1.02g/L on the electron transfer rate fluctuates with time, as shown in Figure 9, the maximum inhibition rate can reach more than 80%, but only at a few time points. Therefore, the inhibition of allelopathic inhibition on the electron transport rate of pseudoantha is weaker than that on its light quantum yield and light energy utilization efficiency.

Half Saturation Light Intensity

Figure 10 is the change curve of half-saturated light intensity of Pseudo Anabaena under different dosages of SKRE, which reflects the tolerance of Pseudo Anabaena to strong light. It can be seen that compared with the control group, the addition of SKRE has a significant effect on The change of half-saturation light intensity had no significant effect, and high concentration dosage would increase the half-saturation light intensity of Pseudomonas anabaena in the later stage of cultivation.

Algal Spontaneous Chlorophyll Fluorescence

The spontaneous chlorophyll fluorescence of algae reflects the situation of algal photosynthetic primordial response to allelopathic stress. Figure 11 shows the changes in the spontaneous chlorophyll fluorescence signal of Pseudo Anabaena under different SKRE dosages. It can be seen that the chlorophyll fluorescence changes in the control group were relatively stable during the growth of Pseudo Anabaena; 0.04 and 0.08g/L The change trend of chlorophyll fluorescence in the dosage group was close to that of the control group. With the increase of dosage, at the initial stage, the 0.17g/L dosage group was slightly lower than the control group, and the 0.25-1.02g/L groups were significantly lower than the control group; with the growth of culture time, the 0.17-1.02g/L dosage group The chlorophyll fluorescence intensity of each experimental group increased significantly, the upward trend of the 0.17-0.34g/L dosage group was the most obvious, and the 0.68g/L experimental group lagged behind, but the chlorophyll fluorescence intensity was the highest after 12 days; 1.02g The increase in /L dosage group was smaller than that of other groups, but it still surpassed the control group between 9-15 days, and then declined, and it was the lowest among all experimental groups on the 18th day.

To sum up, it can be seen that the impact of Helicodium chinensis on algae first acts on the photosynthetic system of algae, affecting the photon yield and photoreaction efficiency of the photosynthetic system. Inhibited and spontaneous chlorophyll fluorescence showed an upward trend over the control group after a period of culture. Further increasing the dosage will cause damage to photosynthetic pigments, thereby achieving the effect of reducing algae biomass.

Example 2

Take the dry root (500g) of the mature Spathiphyllum Kochii plant, remove the pollutants on the surface of litchi leaves (200g), dry the root of reed (300g) and grind them into powder, and weigh the hydroxypropyl group according to the ratio of 100:2. Starch is added to the aqueous solution to obtain a mixed solution, and the obtained mixed solution is added to a high-pressure spray device, and the mixed solution is prepared into tiny particles by spraying, and the tiny particles are adsorbed on the surface of the zeolite, and baked at a low temperature Get algae inhibitors.

Embed the obtained algae inhibitor into the mesh on the plastic plate, put it into the reservoir containing Pseudo-Anabaena, and carry out sampling analysis at regular intervals. After comparison, it is found that the algae inhibitor has a good effect on the growth of cyanobacteria. inhibitory effect.

It should be understood that the application of the present invention is not limited to the above examples, and those skilled in the art can make improvements or transformations according to the above descriptions, and all these improvements and transformations should belong to the protection scope of the appended claims of the present invention.

Claims

A preparation method for algae inhibitor, characterized in that, comprising:

provide a carrier;

Adding herbal medicine powder and filler to the solvent to obtain a mixed solution;

After the mixed solution is sprayed on the surface of the carrier and dried, the algae inhibitor is obtained.
The preparation method according to claim 1, characterized in that, the plant medicinal material is selected from one or more of crane taro, reed, Daping, water sword leaf, litchi leaf, magnolia and renmianzi.
The preparation method according to claim 1, wherein the carrier is selected from one of zeolites, carbon nanotubes and organic framework materials.
The preparation method according to claim 3, wherein the step of obtaining the algae inhibitor after spraying the mixed solution on the surface of the carrier and drying it specifically comprises:

Atomizing the mixed solution to form particles, and attaching the particles to the surface of the carrier to obtain a semi-finished product;

The semi-finished product is put into drying equipment to dry to obtain the algae inhibitor.
The preparation method according to claim 1, wherein the solvent is water.
The preparation method according to claim 1, wherein the filler is hydroxypropyl starch and/or hypromellose.
The preparation method according to claim 1, characterized in that, the mass ratio of the plant medicinal material powder to the filler is 100:0.1-5.
The preparation method according to claim 4, characterized in that the particle size of the particles is 50-100nm.
An algae inhibitor, characterized in that it is prepared by the preparation method described in any one of claims 1-8.
The algae inhibitor according to claim 9, characterized in that, the algae inhibitor also includes a base material for carrying the algae inhibitor, the base material is provided with several mesh holes, and the algae inhibitor is fixed within the mesh.