WO2022215983A1

WO2022215983A1 - Metal oxide nano-composite structure and forming method therefor

Info

Publication number: WO2022215983A1
Application number: PCT/KR2022/004817
Authority: WO
Inventors: 현택환; 김영건
Original assignee: 서울대학교산학협력단; 기초과학연구원
Priority date: 2021-04-09
Filing date: 2022-04-04
Publication date: 2022-10-13
Also published as: KR102506717B1; KR20220140937A

Abstract

Provided are a metal oxide nano-composite structure and a forming method therefor. The metal oxide nano-composite structure comprises a metal oxide nano-structure and a ligand compound bound to the metal oxide nano-structure. The forming method for the metal oxide nano-composite structure comprises the steps of: forming a precursor solution containing a metal precursor and a ligand compound; and forming a metal oxide nano-composite structure by addition of a base to the precursor solution.

Description

Metal oxide nanocomposite structure and method for forming the same

The present invention relates to a metal oxide nanocomposite structure and a method for forming the same.

Conventional synthesis of inorganic nanomaterials is not only complicated in the synthesis process such as being performed in an inert gas or vacuum at high temperature with an organic solvent, but also nanomaterial synthesis in organic solvents is toxic by using surfactants that are harmful to the human body. There is a problem that the use of the substance is limited.

The present invention provides a metal oxide nanocomposite structure having excellent performance.

The present invention provides a method of forming a metal oxide nanocomposite structure.

Other objects of the present invention will become apparent from the following detailed description and accompanying drawings.

The metal oxide nanocomposite structure according to embodiments of the present invention includes a metal oxide nanostructure and a ligand compound coupled to the metal oxide nanostructure.

The metal oxide nanostructure may include at least one of metal oxide nanoclusters and metal oxide nanoparticles.

The metal oxide nanostructure may include at least one of a single metal oxide nanostructure and a multimetal oxide nanostructure. The single metal oxide nanostructure may include one of cerium, manganese, and iron, and the multimetal oxide nanostructure may include two or more of cerium, manganese, and iron.

The ligand compound may include a polymer compound, and the metal oxide nanostructure may be dispersed in the polymer compound.

The ligand compound may include a polymer compound, and the metal oxide nanostructure may be surrounded by the polymer compound.

The ligand compound may include at least one of polysaccharide and albumin. The ligand compound may include at least one of a hydroxyl group and a carboxyl group. The type of the ligand compound may be determined according to the type of the metal oxide.

A method of forming a metal oxide nanocomposite structure according to embodiments of the present invention includes forming a precursor solution including a metal precursor and a ligand compound, and adding a base to the precursor solution to form a metal oxide nanocomposite structure includes The metal oxide nanocomposite structure includes a metal oxide nanostructure and a ligand compound bonded to the metal oxide nanostructure.

The metal of the metal precursor may be precipitated by the addition of the base to form the metal oxide nanostructure.

The metal precursor may include at least one of a cerium precursor, a manganese precursor, and an iron precursor.

The method of forming the metal oxide nanocomposite structure may further include separating the metal oxide nanocomposite structure from the precursor solution using a membrane filter. The precursor solution may be an aqueous solution. The base may include at least one of NH ₄ OH and KOH.

Metal oxide nanocomposite structures according to embodiments of the present invention may have excellent performance. For example, the metal oxide nanocomposite structure may have excellent biocompatibility and antioxidant performance. In addition, the metal oxide nanocomposite structure can be formed in large quantities in a simple method in water without using an organic solvent.

1 and 2 are views for explaining a method of forming a metal oxide nanocomposite structure according to embodiments of the present invention.

3 to 5 show the results of analyzing the performance of the metal oxide nanocomposite structure (CeMn/HSA) according to an embodiment of the present invention.

6 to 8 show the results of analyzing the performance of the metal oxide nanocomposite structure according to embodiments of the present invention.

Hereinafter, the present invention will be described in detail through examples. Objects, features, and advantages of the present invention will be readily understood through the following examples. The present invention is not limited to the embodiments described herein, and may be embodied in other forms. The embodiments introduced herein are provided so that the disclosed content can be thorough and complete, and the spirit of the present invention can be sufficiently conveyed to those of ordinary skill in the art to which the present invention pertains. Therefore, the present invention should not be limited by the following examples.

In the present specification, the metal oxide nanocomposite structure may be expressed as A/B. A represents a metal oxide nanostructure, and B represents a ligand compound bound to A. For example, in Ce/HSA, Ce represents a cerium oxide nanostructure, and HSA represents albumin, which is a ligand compound bound to the cerium oxide nanostructure. In Mn/DEX, Mn represents a manganese oxide nanostructure, and DEX represents dextran, which is a ligand compound bound to the manganese oxide nanostructure. In Fe/HSA-DEX, Fe represents an iron oxide nanostructure, and HSA-DEX represents albumin and dextran, which are ligand compounds bound to the manganese oxide nanostructure. In CeMn/HSA, CeMn represents a double metal oxide nanostructure including Ce and Mn, and HSA represents albumin, which is a ligand compound bound to the double metal oxide nanostructure. In MnFe/DEX, MnFe represents a double metal oxide nanostructure including Mn and Fe, and DEX represents dextran, which is a ligand compound bonded to the double metal oxide nanostructure. CeFe/HSA-DEX represents a double metal oxide nanostructure including Ce and Fe, and HSA-DEX represents albumin and dextran, which are ligand compounds bound to the double metal oxide nanostructure.

1 and 2 , the method of forming a metal oxide nanocomposite structure includes forming a precursor solution (S10), forming a metal oxide nanocomposite structure (S20), and separating the metal oxide nanocomposite structure It may include a step (S30).

A precursor solution including a metal precursor and a ligand compound is formed (S10).

The metal precursor may include at least one of a Ce precursor, a Mn precursor, and an Fe precursor. The type of the metal precursor included in the precursor solution may be determined according to the type of metal oxide included in the metal oxide nanocomposite structure to be formed. For example, when the metal oxide nanocomposite structure includes cerium oxide and manganese oxide, the metal precursor may include a Ce precursor and a Mn precursor, and when the metal oxide nanocomposite structure includes cerium oxide and iron oxide The metal precursor may include a Ce precursor and an Fe precursor. The Ce precursor may include CeCl ₃ or CeNO ₃ , the Mn precursor may include MnCl ₂ , and the Fe precursor may include FeCl ₂ .

The ligand compound may include a polymer compound. The high molecular compound may include at least one of polysaccharide and albumin. For example, the polysaccharide may include dextran (DEX), and the albumin may include human serum albumin (HSA). The ligand compound may include at least one of a hydroxyl group and a carboxyl group. The type of the ligand compound included in the precursor solution may be determined according to the type of metal oxide included in the metal oxide nanocomposite structure to be formed.

A base is added to the precursor solution to form a metal oxide nanocomposite structure (S20).

The metal of the metal precursor may be precipitated by the addition of the base to the precursor solution to form a metal oxide nanostructure, and finally a metal oxide nanocomposite structure may be formed. The base may include at least one of NH ₄ OH and KOH.

The metal oxide nanocomposite structure is separated from the precursor solution using a membrane filter (S30).

Before separating the metal oxide nanocomposite structure, washing may be performed using a centrifugal filter. The metal precursor, ligand compound, and base remaining in the precursor solution (precursor solution after washing) are removed using the membrane filter, and only the metal oxide nanocomposite structure can be easily extracted.

The metal oxide nanocomposite structure includes a metal oxide nanostructure and a ligand compound bonded to the metal oxide nanostructure. The metal oxide nanostructure may include at least one of metal oxide nanoclusters and metal oxide nanoparticles. In addition, the metal oxide nanostructure may exist in an amorphous state, a crystalline state, or a mixed state of amorphous and crystalline.

The ligand compound may include a polymer compound, and the metal oxide nanostructure may be dispersed in the polymer compound. In addition, the ligand compound may include a polymer compound, and the metal oxide nanostructure may be surrounded by the polymer compound. The metal oxide nanocomposite structure may include one or more metal oxide nanostructures, and the metal oxide nanostructure and the ligand compound may have various bonding forms. The bonding form of the metal oxide nanostructure and the ligand compound may be controlled by the type and concentration of the metal precursor, the type and concentration of the ligand compound, the type and concentration of the base, the reaction time, and the like.

The metal oxide nanostructure may include at least one of a single metal oxide nanostructure and a multimetal oxide nanostructure. When the precursor solution includes one metal precursor, the metal oxide nanocomposite structure may include a single metal oxide nanostructure, and when the precursor solution includes two or more metal precursors, the metal oxide nanocomposite structure includes multiple It may include a metal oxide nanostructure. When the precursor solution includes a Ce precursor and a Mn precursor The metal oxide nanocomposite structure may include a double metal oxide nanostructure including Ce and Mn, and when the precursor solution includes a Ce precursor and a Fe precursor The metal oxide nanocomposite structure may include a double metal oxide nanostructure containing Ce and Fe, and when the precursor solution includes a Mn precursor and an Fe precursor, the metal oxide nanocomposite structure includes Mn and Fe It may include a double metal oxide nanostructure. In addition, when the precursor solution includes a Ce precursor, a Mn precursor, and an Fe precursor, the metal oxide nanocomposite structure may include a triple metal oxide nanostructure including Ce, Mn, and Fe.

The multi-metal oxide nanostructure may be formed by co-precipitating two or more metals in Ce, Mn, and Fe. As described above, the multi-metal oxide formed through the co-precipitation reaction has various oxidation states (Oxygen state), unlike when only one type of metal is precipitated, and the oxygen vacancy increases so that electron movement is active. happens to happen That is, the multi-metal oxide nanostructure may have improved active oxygen decomposition performance and improved antioxidant performance through co-precipitation of two or more of Ce, Mn, and Fe.

In addition, while cerium oxide nanoparticles have poor biodegradability, manganese and iron exist in the body, and manganese oxide and iron oxide have high biodegradability. Glutathione (GSH), which helps the excretion by causing biotransformation of foreign substances in the liver, reduces manganese oxide and iron oxide, decomposes them into ions, and discharges them outside the body. When cerium is co-precipitated together with manganese or iron to form a multi-metal oxide nanostructure, manganese oxide and iron oxide coexisting with cerium oxide are decomposed by glutathione (GSH), and cerium oxide is also discharged to improve biodegradability. can

In order to use the metal oxide nanostructure in a living body for medical purposes, the degree of dispersion is important. Even when high-performance nanomaterials are synthesized, if agglomeration occurs in PBS, serum, and blood used in actual clinical practice other than distilled water, the active site on the surface of the nanomaterial is reduced, thereby reducing commercial value. In the metal oxide nanocomposite structure, a ligand compound is defective in the metal oxide nanostructure, thereby preventing aggregation of the metal oxide nanostructure and increasing dispersibility. In particular, by using albumin and/or polysaccharide as the ligand compound, the metal oxide nanocomposite structure has an electron repulsion force and a steric interference effect to maintain the dispersion degree of the metal oxide nanostructure in an aqueous solution having various ionic strengths. In addition, it may have biocompatibility. In particular, polysaccharides such as dextran can effectively prevent aggregation of metal oxide nanostructures by increasing the steric interference effect even with respect to multi-metal oxides (CeMnO _x , CeFeO _x , MnFeO _x , CeMnFeO _x ).

[Formation example of Ce/HSA]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. Albumin (Human Serum Albumin, HSA) powder 200mg is dissolved in 5ml of distilled water to form an aqueous albumin solution. A precursor aqueous solution is formed by mixing an aqueous solution of CeCl ₃ ·7H ₂ O and an aqueous albumin solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Ce. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A cerium oxide nanostructure is formed by the reaction, and finally a metal oxide nanocomposite structure (Ce/HSA) is formed. Ce/HSA includes cerium oxide nanostructures and albumin bound to the cerium oxide nanostructures. The cerium oxide nanostructure may include at least one of cerium oxide nanoclusters and cerium oxide nanoparticles.

The solution containing Ce/HSA was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered through a 0.22 μm membrane filter to separate Ce/HSA.

[Formation example of Ce/DEX]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. Dextran (DEX) powder 200mg is dissolved in 5ml of distilled water to form an aqueous solution of dextran. CeCl ₃ ·7H ₂ O aqueous solution and dextran aqueous solution are mixed to form a precursor aqueous solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Ce. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A cerium oxide nanostructure is formed by the reaction, and a metal oxide nanocomposite structure (Ce/DEX) is finally formed. Ce/DEX includes cerium oxide nanostructures and dextran bound to the cerium oxide nanostructures. The cerium oxide nanostructure may include at least one of cerium oxide nanoclusters and cerium oxide nanoparticles.

The solution containing Ce/DEX is washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate Ce/DEX.

[Formation example of Ce/HSA-DEX]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. 100 mg of albumin powder and 100 mg of dextran powder are dissolved in 5 ml of distilled water to form an albumin-dextran aqueous solution. CeCl ₃ ·7H ₂ O aqueous solution and albumin-dextran aqueous solution are mixed to form a precursor aqueous solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Ce. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A cerium oxide nanostructure is formed by the reaction, and finally a metal oxide nanocomposite structure (Ce/HSA-DEX) is formed. Ce/HSA-DEX contains cerium oxide nanostructures and albumin and dextran bound to the cerium oxide nanostructures. The cerium oxide nanostructure may include at least one of cerium oxide nanoclusters and cerium oxide nanoparticles.

The solution containing Ce/HSA-DEX is washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate Ce/HSA-DEX.

[Formation example of Mn/HSA]

Prepare 0.1M MnCl ₂ ·4H ₂ O aqueous solution. 200 mg of albumin powder is dissolved in 5 ml of distilled water to form an aqueous albumin solution. A precursor aqueous solution is formed by mixing an aqueous solution of MnCl ₂ ·4H ₂ O and an aqueous albumin solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Mn. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A manganese oxide nanostructure is formed by the reaction, and finally a metal oxide nanocomposite structure (Mn/HSA) is formed. Mn/HSA includes manganese oxide nanostructures and albumin bound to the manganese oxide nanostructures. The manganese oxide nanostructure may include at least one of manganese oxide nanoclusters and manganese oxide nanoparticles.

The solution containing Mn/HSA was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered through a 0.22 μm membrane filter to separate Mn/HSA.

[Formation example of Mn/DEX]

Prepare 0.1M MnCl ₂ ·4H ₂ O aqueous solution. Dissolve 200 mg of dextran powder in 5 ml of distilled water to form an aqueous solution of dextran. A precursor aqueous solution is formed by mixing an aqueous MnCl ₂ ·4H ₂ O solution and an aqueous dextran solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Mn. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A manganese oxide nanostructure is formed by the above reaction, and finally a metal oxide nanocomposite structure (Mn/DEX) is formed. Mn/DEX includes manganese oxide nanostructures and dextran bound to the manganese oxide nanostructures. The manganese oxide nanostructure may include at least one of manganese oxide nanoclusters and manganese oxide nanoparticles.

The solution containing Mn/DEX is washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered through a 0.22 μm membrane filter to separate Mn/DEX.

[Formation example of Mn/HSA-DEX]

Prepare 0.1M MnCl ₂ ·4H ₂ O aqueous solution. 100 mg of albumin powder and 100 mg of dextran powder are dissolved in 5 ml of distilled water to form an albumin-dextran aqueous solution. MnCl ₂ ·4H ₂ O aqueous solution and albumin-dextran aqueous solution are mixed to form a precursor aqueous solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Mn. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A manganese oxide nanostructure is formed by the reaction, and finally a metal oxide nanocomposite structure (Mn/HSA-DEX) is formed. Mn/HSA-DEX includes manganese oxide nanostructures and albumin and dextran bound to the manganese oxide nanostructures. The manganese oxide nanostructure may include at least one of manganese oxide nanoclusters and manganese oxide nanoparticles.

The solution containing Mn/HSA-DEX is washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. Mn/HSA-DEX is separated by filtering the washed solution with a 0.22 μm membrane filter.

[Formation example of Fe/HSA]

Prepare 0.1M FeCl ₂ ·4H ₂ O aqueous solution. 200 mg of albumin powder is dissolved in 5 ml of distilled water to form an aqueous albumin solution. A precursor aqueous solution is formed by mixing an aqueous solution of FeCl ₂ ·4H ₂ O and an aqueous albumin solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. An iron oxide nanostructure is formed by the reaction, and finally a metal oxide nanocomposite structure (Fe/HSA) is formed. Fe/HSA includes iron oxide nanostructures and albumin bound to the iron oxide nanostructures. The iron oxide nanostructure may include at least one of iron oxide nanoclusters and iron oxide nanoparticles.

The solution containing Fe/HSA was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered through a 0.22 μm membrane filter to separate Fe/HSA.

[Formation example of Fe/DEX]

Prepare 0.1M FeCl ₂ ·4H ₂ O aqueous solution. Dissolve 200 mg of dextran powder in 5 ml of distilled water to form an aqueous solution of dextran. A precursor aqueous solution is formed by mixing the FeCl ₂ ·4H ₂ O aqueous solution and the dextran aqueous solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. An iron oxide nanostructure is formed by the reaction, and finally a metal oxide nanocomposite structure (Fe/DEX) is formed. Fe/DEX includes iron oxide nanostructures and dextran bound to the iron oxide nanostructures. The iron oxide nanostructure may include at least one of iron oxide nanoclusters and iron oxide nanoparticles.

The solution containing Fe/DEX is washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate Fe/DEX.

[Formation example of Fe/HSA-DEX]

Prepare 0.1M FeCl ₂ ·4H ₂ O aqueous solution. 100 mg of albumin powder and 100 mg of dextran powder are dissolved in 5 ml of distilled water to form an albumin-dextran aqueous solution. FeCl ₂ ·4H ₂ O aqueous solution and albumin-dextran aqueous solution is mixed to form a precursor aqueous solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. An iron oxide nanostructure is formed by the reaction, and finally a metal oxide nanocomposite structure (Fe/HSA-DEX) is formed. Fe/HSA-DEX includes manganese oxide nanostructures and albumin and dextran bound to the iron oxide nanostructures. The iron oxide nanostructure may include at least one of iron oxide nanoclusters and iron oxide nanoparticles.

The solution containing Fe/HSA-DEX is washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate Fe/HSA-DEX.

[CeMn/HSA Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution and 0.1M MnCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. 250 μl of CeCl ₃ ·7H ₂ O aqueous solution and 250 μl of MnCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of CeMn aqueous solution. In consideration of the ratio of Ce ³⁺ and Mn ²⁺ in the CeMn aqueous solution, the amounts of the CeCl ₃ ·7H ₂ O aqueous solution and the MnCl ₂ ·4H ₂ O aqueous solution can be adjusted, and Ce included in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Mn can be adjusted. 200 mg of albumin powder is dissolved in 5 ml of distilled water to form an aqueous albumin solution. An aqueous solution of CeMn and an aqueous solution of albumin are mixed to form a precursor aqueous solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Ce and Mn. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure including Ce and Mn is formed, and finally a metal oxide nanocomposite structure (CeMn/HSA) is formed. CeMn/HSA includes a double metal oxide nanostructure including Ce and Mn and albumin bound to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing CeMn/HSA was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate CeMn/HSA.

[CeMn/DEX Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution and 0.1M MnCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. 250 μl of CeCl ₃ ·7H ₂ O aqueous solution and 250 μl of MnCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of CeMn aqueous solution. In consideration of the ratio of Ce ³⁺ and Mn ²⁺ in the CeMn aqueous solution, the amounts of the CeCl ₃ ·7H ₂ O aqueous solution and the MnCl ₂ ·4H ₂ O aqueous solution can be adjusted, and Ce included in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Mn can be adjusted. Dissolve 200 mg of dextran powder in 5 ml of distilled water to form an aqueous solution of dextran. An aqueous solution of CeMn and an aqueous solution of dextran are mixed to form a precursor aqueous solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Ce and Mn. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure containing Ce and Mn is formed, and finally a metal oxide nanocomposite structure (CeMn/DEX) is formed. CeMn/DEX includes a double metal oxide nanostructure including Ce and Mn and dextran bonded to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing CeMn/DEX was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered through a 0.22 μm membrane filter to separate CeMn/DEX.

[CeMn/HSA-DEX Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution and 0.1M MnCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. 250 μl of CeCl ₃ ·7H ₂ O aqueous solution and 250 μl of MnCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of CeMn aqueous solution. In consideration of the ratio of Ce ³⁺ and Mn ²⁺ in the CeMn aqueous solution, the amounts of the CeCl ₃ ·7H ₂ O aqueous solution and the MnCl ₂ ·4H ₂ O aqueous solution can be adjusted, and Ce included in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Mn can be adjusted. 100 mg of albumin powder and 100 mg of dextran powder are dissolved in 5 ml of distilled water to form an albumin-dextran aqueous solution. An aqueous solution of CeMn and an aqueous solution of albumin-dextran are mixed to form an aqueous precursor solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Ce and Mn. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure including Ce and Mn is formed, and finally a metal oxide nanocomposite structure (CeMn/HSA-DEX) is formed. CeMn/HSA-DEX includes a double metal oxide nanostructure including Ce and Mn, and albumin and dextran bound to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing CeMn/HSA-DEX was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate CeMn/HSA-DEX.

[Example of MnFe/HSA formation]

Prepare 0.1M MnCl ₂ ·4H ₂ O aqueous solution and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. 250 μl of MnCl ₂ ·4H ₂ O aqueous solution and 250 μl of FeCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of MnFe aqueous solution. Considering the ratio of Mn ²⁺ and Fe ²⁺ in the MnFe aqueous solution, the amounts of the MnCl ₂ ·4H ₂ O aqueous solution and the FeCl ₂ ·4H ₂ O aqueous solution can be adjusted, and Mn contained in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Fe can be adjusted. 200 mg of albumin powder is dissolved in 5 ml of distilled water to form an aqueous albumin solution. A precursor aqueous solution is formed by mixing an aqueous MnFe solution and an aqueous albumin solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Mn and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure containing Mn and Fe is formed, and finally a metal oxide nanocomposite structure (MnFe/HSA) is formed. MnFe/HSA includes a double metal oxide nanostructure including Mn and Fe, and albumin bound to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing MnFe/HSA was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate MnFe/HSA.

[Example of MnFe/DEX formation]

Prepare 0.1M MnCl ₂ ·4H ₂ O aqueous solution and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. 250 μl of MnCl ₂ ·4H ₂ O aqueous solution and 250 μl of FeCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of MnFe aqueous solution. Considering the ratio of Mn ²⁺ and Fe ²⁺ in the MnFe aqueous solution, the amounts of the MnCl ₂ ·4H ₂ O aqueous solution and the FeCl ₂ ·4H ₂ O aqueous solution can be adjusted, and Mn contained in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Fe can be adjusted. Dissolve 200 mg of dextran powder in 5 ml of distilled water to form an aqueous solution of dextran. A precursor aqueous solution is formed by mixing an aqueous MnFe solution and an aqueous dextran solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Mn and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure containing Mn and Fe is formed, and finally a metal oxide nanocomposite structure (MnFe/DEX) is formed. MnFe/DEX includes a double metal oxide nanostructure including Mn and Fe and dextran bonded to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing MnFe/DEX was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. MnFe/DEX is separated by filtering the washed solution with a 0.22 μm membrane filter.

[Example of MnFe/HSA-DEX formation]

Prepare 0.1M MnCl ₂ ·4H ₂ O aqueous solution and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. 250 μl of MnCl ₂ ·4H ₂ O aqueous solution and 250 μl of FeCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of MnFe aqueous solution. Considering the ratio of Mn ²⁺ and Fe ²⁺ in the MnFe aqueous solution, the amounts of the MnCl ₂ ·4H ₂ O aqueous solution and the FeCl ₂ ·4H ₂ O aqueous solution can be adjusted, and Mn contained in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Fe can be adjusted. 100 mg of albumin powder and 100 mg of dextran powder are dissolved in 5 ml of distilled water to form an albumin-dextran aqueous solution. A precursor aqueous solution is formed by mixing an aqueous MnFe solution and an aqueous albumin-dextran solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Mn and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure containing Mn and Fe is formed, and finally a metal oxide nanocomposite structure (MnFe/HSA-DEX) is formed. MnFe/HSA-DEX includes a double metal oxide nanostructure including Mn and Fe, and albumin and dextran bonded to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing MnFe/HSA-DEX is washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate MnFe/HSA-DEX.

[CeFe/HSA Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. 250 μl of CeCl ₃ ·7H ₂ O aqueous solution and 250 μl of FeCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of CeFe aqueous solution. The amount of CeCl ₃ ·7H ₂ O aqueous solution and FeCl ₂ ·4H ₂ O aqueous solution can be adjusted in consideration of the ratio of Ce ³⁺ and Fe ²⁺ in the CeFe aqueous solution, and Ce included in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Fe can be adjusted. 200 mg of albumin powder is dissolved in 5 ml of distilled water to form an aqueous albumin solution. An aqueous solution of CeFe and an aqueous solution of albumin are mixed to form an aqueous precursor solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Ce and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure containing Ce and Fe is formed, and finally a metal oxide nanocomposite structure (CeFe/HSA) is formed. CeFe/HSA includes a double metal oxide nanostructure including Ce and Fe, and albumin bound to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing CeFe/HSA was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate CeFe/HSA.

[CeFe/DEX Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. 250 μl of CeCl ₃ ·7H ₂ O aqueous solution and 250 μl of FeCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of CeFe aqueous solution. The amount of CeCl ₃ ·7H ₂ O aqueous solution and FeCl ₂ ·4H ₂ O aqueous solution can be adjusted in consideration of the ratio of Ce ³⁺ and Fe ²⁺ in the CeFe aqueous solution, and Ce included in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Fe can be adjusted. Dissolve 200 mg of dextran powder in 5 ml of distilled water to form an aqueous solution of dextran. A precursor aqueous solution is formed by mixing the CeFe aqueous solution and the dextran aqueous solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Ce and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure containing Ce and Fe is formed, and finally a metal oxide nanocomposite structure (CeFe/DEX) is formed. CeFe/DEX includes a double metal oxide nanostructure including Ce and Fe, and dextran bonded to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing CeFe/DEX was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered through a 0.22 μm membrane filter to separate CeFe/DEX.

[CeFe/HSA-DEX Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. 250 μl of CeCl ₃ ·7H ₂ O aqueous solution and 250 μl of FeCl ₂ ·4H ₂ O aqueous solution are mixed to form 500 μl of CeFe aqueous solution. The amount of CeCl ₃ ·7H ₂ O aqueous solution and FeCl ₂ ·4H ₂ O aqueous solution can be adjusted in consideration of the ratio of Ce ³⁺ and Fe ²⁺ in the CeFe aqueous solution, and Ce included in the metal oxide nanocomposite structure formed thereby The amount and ratio of and Fe can be adjusted. 100 mg of albumin powder and 100 mg of dextran powder are dissolved in 5 ml of distilled water to form an albumin-dextran aqueous solution. An aqueous solution of the precursor is formed by mixing an aqueous solution of CeFe and an aqueous albumin-dextran solution.

200 μl of NH ₄ OH (28-30%) solution was added to the aqueous precursor solution to perform a precipitation reaction of Ce and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a double metal oxide nanostructure containing Ce and Fe is formed, and finally a metal oxide nanocomposite structure (CeFe/HSA-DEX) is formed. CeFe/HSA-DEX includes a double metal oxide nanostructure including Ce and Fe, and albumin and dextran bonded to the double metal oxide nanostructure. The double metal oxide nanostructure may include at least one of double metal oxide nanoclusters and double metal oxide nanoparticles.

The solution containing CeFe/HSA-DEX was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate CeFe/HSA-DEX.

[CeMnFe/HSA Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution, 0.1 M MnCl ₂ ·4H ₂ O aqueous solution, and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. CeMnFe aqueous solution is formed by mixing 167 μl of CeCl ₃ ·7H ₂ O aqueous solution, 167 μl of MnCl ₂ ·4H ₂ O aqueous solution, and 167 μl of FeCl ₂ ·4H ₂ O aqueous solution. The amounts of CeCl ₃ ·7H ₂ O aqueous solution, MnCl ₂ ·4H ₂ O aqueous solution, and FeCl ₂ ·4H ₂ O aqueous solution can be adjusted by considering the ratio of Ce ³⁺ , Mn ²⁺ , and Fe ²⁺ in the CeMnFe aqueous solution, , it is possible to control the amount and ratio of Ce, Mn, and Fe included in the metal oxide nanocomposite structure formed by this. 200 mg of albumin powder is dissolved in 5 ml of distilled water to form an aqueous albumin solution. A precursor aqueous solution is formed by mixing an aqueous solution of CeMnFe and an aqueous albumin solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Ce, Mn, and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. By the reaction, a triple metal oxide nanostructure including Ce, Mn, and Fe is formed, and finally a metal oxide nanocomposite structure (CeMnFe/HSA) is formed. CeMnFe/HSA includes a triple metal oxide nanostructure including Ce, Mn, and Fe, and albumin bound to the triple metal oxide nanostructure. The triple metal oxide nanostructure may include at least one of a triple metal oxide nanocluster and a triple metal oxide nanoparticle.

The solution containing CeMnFe/HSA was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution was filtered with a 0.22 μm membrane filter to separate CeMnFe/HSA.

[CeMnFe/DEX Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution, 0.1 M MnCl ₂ ·4H ₂ O aqueous solution, and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. CeMnFe aqueous solution is formed by mixing 167 μl of CeCl ₃ ·7H ₂ O aqueous solution, 167 μl of MnCl ₂ ·4H ₂ O aqueous solution, and 167 μl of FeCl ₂ ·4H ₂ O aqueous solution. The amounts of CeCl ₃ ·7H ₂ O aqueous solution, MnCl ₂ ·4H ₂ O aqueous solution, and FeCl ₂ ·4H ₂ O aqueous solution can be adjusted by considering the ratio of Ce ³⁺ , Mn ²⁺ , and Fe ²⁺ in the CeMnFe aqueous solution, , it is possible to control the amount and ratio of Ce, Mn, and Fe included in the metal oxide nanocomposite structure formed by this. Dissolve 200 mg of dextran powder in 5 ml of distilled water to form an aqueous solution of dextran. An aqueous solution of a precursor is formed by mixing an aqueous solution of CeMnFe and an aqueous solution of dextran.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Ce, Mn, and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A triple metal oxide nanostructure including Ce, Mn, and Fe is formed by the reaction, and finally a metal oxide nanocomposite structure (CeMnFe/DEX) is formed. CeMnFe/DEX includes a triple metal oxide nanostructure including Ce, Mn, and Fe, and dextran bonded to the triple metal oxide nanostructure. The triple metal oxide nanostructure may include at least one of a triple metal oxide nanocluster and a triple metal oxide nanoparticle.

The solution containing CeMnFe/DEX was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered through a 0.22 μm membrane filter to separate CeMnFe/DEX.

[CeMnFe/HSA-DEX Formation Example]

Prepare 0.1M CeCl ₃ ·7H ₂ O aqueous solution, 0.1 M MnCl ₂ ·4H ₂ O aqueous solution, and 0.1M FeCl ₂ ·4H ₂ O aqueous solution. Instead of CeCl ₃ ·7H ₂ O, CeNO ₃ ·6H ₂ O may be used. CeMnFe aqueous solution is formed by mixing 167 μl of CeCl ₃ ·7H ₂ O aqueous solution, 167 μl of MnCl ₂ ·4H ₂ O aqueous solution, and 167 μl of FeCl ₂ ·4H ₂ O aqueous solution. The amounts of CeCl ₃ ·7H ₂ O aqueous solution, MnCl ₂ ·4H ₂ O aqueous solution, and FeCl ₂ ·4H ₂ O aqueous solution can be adjusted by considering the ratio of Ce ³⁺ , Mn ²⁺ , and Fe ²⁺ in the CeMnFe aqueous solution, , it is possible to control the amount and ratio of Ce, Mn, and Fe included in the metal oxide nanocomposite structure formed by this. 100 mg of albumin powder and 100 mg of dextran powder are dissolved in 5 ml of distilled water to form an albumin-dextran aqueous solution. A precursor aqueous solution is formed by mixing an aqueous solution of CeMnFe and an aqueous albumin-dextran solution.

200 μl of NH ₄ OH (28-30%) solution is added to the aqueous precursor solution to perform a precipitation reaction of Ce, Mn, and Fe. In the above reaction, KOH may be used instead of NH ₄ OH. The precipitation reaction is performed at room temperature for 2 hours while shaking the aqueous precursor solution at about 300 rpm. A triple metal oxide nanostructure including Ce, Mn, and Fe is formed by the reaction, and finally a metal oxide nanocomposite structure (CeMnFe/HSA-DEX) is formed. CeMnFe/HSA-DEX includes a triple metal oxide nanostructure including Ce, Mn, and Fe, and albumin and dextran bonded to the triple metal oxide nanostructure. The triple metal oxide nanostructure may include at least one of a triple metal oxide nanocluster and a triple metal oxide nanoparticle.

The solution containing CeMnFe/HSA-DEX was washed 4 times with distilled water at 5,000 rpm for 10 minutes using a 100 kDa centrifugal filter. The washed solution is filtered with a 0.22 μm membrane filter to separate CeMnFe/HSA-DEX.

3 to 5 show the results of analyzing the performance of the metal oxide nanocomposite structure (CeMn/HSA) according to an embodiment of the present invention. 3 shows the results of the cytotoxicity analysis of CeMn/HSA, FIG. 4 shows the results of the antioxidant performance analysis of CeMn/HSA, and FIG. 5 shows the results of the biodegradability analysis of CeMn/HSA. 3 to 5, CM75 represents a double metal oxide nanocomposite structure having a Ce:Mn content ratio of 75:25, CM50 represents a double metal oxide nanocomposite structure having a Ce:Mn content ratio of 50:50, and CM25 is A double metal oxide nanocomposite structure having a Ce:Mn content ratio of 25:75 is shown.

Referring to FIG. 3 , the cytotoxicity of the double metal oxide nanocomposite structure containing Ce and Mn is similar to or smaller than the cytotoxicity of the single metal oxide nanocomposite structure containing only Ce or Mn.

Referring to FIG. 4 , the antioxidant performance of the double metal oxide nanocomposite structure containing Ce and Mn is superior to the antioxidant performance of the single metal oxide nanocomposite structure containing only Ce or Mn.

Referring to FIG. 5 , the biodegradability of the double metal oxide nanocomposite structure containing Ce and Mn is higher than that of the single metal oxide nanocomposite structure containing only Ce.

6 to 8 show the results of analyzing the performance of the metal oxide nanocomposite structure according to embodiments of the present invention. CM50 represents a double metal oxide nanocomposite structure having a Ce:Mn content ratio of 50:50, MF50 represents a double metal oxide nanocomposite structure having a Mn:Fe content ratio of 50:50, and CF50 is a Ce:Fe content ratio A 50:50 double metal oxide nanocomposite structure is shown.

6, the double metal oxide nanocomposite structure has higher SOD activity than the single metal oxide nanocomposite structure, and the double metal oxide nanocomposite structure containing Ce and Mn is CAT than the single metal oxide nanocomposite structure containing only Ce. activity is high. Overall, the double metal oxide nanocomposite structure has better antioxidant performance than the single metal oxide nanocomposite structure.

Referring to FIG. 7 , albumin can further increase the dispersibility of the single metal oxide nanocomposite structure than the double metal oxide nanocomposite structure, and dextran shows the dispersibility of the double metal oxide nanocomposite structure than the single metal oxide nanocomposite structure. can be raised further.

Referring to FIG. 8 , dextran having both a hydroxyl group and a carboxyl group may further increase the dispersibility of the metal oxide nanocomposite structure than dextran having only a hydroxyl group.

So far, specific embodiments of the present invention have been described. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims

metal oxide nanostructures; and

A metal oxide nanocomposite structure comprising a ligand compound coupled to the metal oxide nanostructure.
The method of claim 1,

The metal oxide nanostructure is a metal oxide nanocomposite structure comprising at least one of metal oxide nanoclusters and metal oxide nanoparticles.
The method of claim 1,

The metal oxide nanostructure includes at least one of a single metal oxide nanostructure and a multimetal oxide nanostructure,

The single metal oxide nanostructure includes one of cerium, manganese, and iron,

The multi-metal oxide nanostructure is a metal oxide nanocomposite structure, characterized in that it comprises two or more of cerium, manganese, and iron.
The method of claim 1,

The ligand compound includes a polymer compound,

The metal oxide nanostructure is a metal oxide nanocomposite structure, characterized in that dispersed in the polymer compound.
The method of claim 1,

The ligand compound includes a polymer compound,

The metal oxide nanostructure is a metal oxide nanocomposite structure, characterized in that surrounded by the polymer compound.
The method of claim 1,

The ligand compound is a metal oxide nanocomposite structure comprising at least one of polysaccharide and albumin.
The method of claim 1,

The ligand compound is a metal oxide nanocomposite structure comprising at least one of a hydroxyl group and a carboxyl group.
The method of claim 1,

The type of the ligand compound is a metal oxide nanocomposite structure, characterized in that determined according to the type of the metal oxide.
forming a precursor solution including a metal precursor and a ligand compound; and

and adding a base to the precursor solution to form a metal oxide nanocomposite structure,

The metal oxide nanocomposite structure,

metal oxide nanostructures; and

Method of forming a metal oxide nanocomposite structure comprising a ligand compound bound to the metal oxide nanostructure.
10. The method of claim 9,

A method of forming a metal oxide nanocomposite structure, characterized in that the metal of the metal precursor is precipitated by the addition of the base to form the metal oxide nanostructure.
11. The method of claim 10,

The metal oxide nanostructure is a method of forming a metal oxide nanocomposite structure, characterized in that it comprises at least one of metal oxide nanoclusters and metal oxide nanoparticles.
11. The method of claim 10,

The metal precursor is a method of forming a metal oxide nanocomposite structure comprising at least one of a cerium precursor, a manganese precursor, and an iron precursor.
11. The method of claim 10,

The ligand compound includes a polymer compound,

The metal oxide nanostructure is a method of forming a metal oxide nanocomposite structure, characterized in that it is dispersed in the polymer compound.
11. The method of claim 10,

The ligand compound includes a polymer compound,

The metal oxide nanostructure is a method of forming a metal oxide nanocomposite structure, characterized in that surrounded by the polymer compound.
11. The method of claim 10,

The ligand compound is a method of forming a metal oxide nanocomposite structure, characterized in that it comprises at least one of polysaccharide and albumin.
11. The method of claim 10,

The ligand compound is a method of forming a metal oxide nanocomposite structure, characterized in that it comprises at least one of a hydroxyl group and a carboxyl group.
11. The method of claim 10,

The type of the ligand compound is a method of forming a metal oxide nanocomposite structure, characterized in that determined according to the type of the metal oxide.
10. The method of claim 9,

Separating the metal oxide nanocomposite structure from the precursor solution using a membrane filter, characterized in that it further comprises the step of forming a metal oxide nanocomposite structure.
10. The method of claim 9,

The precursor solution is a method of forming a metal oxide nanocomposite structure, characterized in that the aqueous solution.
10. The method of claim 9,

The base is a method of forming a metal oxide nanocomposite structure, characterized in that it comprises at least one of NH 4 OH and KOH.