WO2022213930A1 - Appareil de détection et procédé de détection de billes magnétiques pour l'extraction et l'amplification d'acides nucléiques - Google Patents

Appareil de détection et procédé de détection de billes magnétiques pour l'extraction et l'amplification d'acides nucléiques Download PDF

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Publication number
WO2022213930A1
WO2022213930A1 PCT/CN2022/085077 CN2022085077W WO2022213930A1 WO 2022213930 A1 WO2022213930 A1 WO 2022213930A1 CN 2022085077 W CN2022085077 W CN 2022085077W WO 2022213930 A1 WO2022213930 A1 WO 2022213930A1
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WIPO (PCT)
Prior art keywords
tube
pipette
magnetic bead
needle
air pump
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PCT/CN2022/085077
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English (en)
Chinese (zh)
Inventor
宋祺
高一博
刘裔腾
温维佳
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珠海市尚维高科生物技术有限公司
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Priority claimed from CN202120689520.3U external-priority patent/CN216378192U/zh
Priority claimed from CN202110365658.2A external-priority patent/CN115181662A/zh
Application filed by 珠海市尚维高科生物技术有限公司 filed Critical 珠海市尚维高科生物技术有限公司
Publication of WO2022213930A1 publication Critical patent/WO2022213930A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters

Definitions

  • the invention relates to the technical field of biological nucleic acid detection, in particular to a detection device and a detection method for nucleic acid extraction and amplification by magnetic bead method.
  • POCT Point of Care Testing
  • molecular detection is the direct detection of pathogenic nucleic acid, which has the characteristics of high sensitivity and high accuracy.
  • the current nucleic acid detection method is mainly to collect samples by professional personnel, and then transport them to the central laboratory for centralized testing.
  • the transportation of samples also requires cold chain transportation, which increases the difficulty and cost of sample delivery.
  • the central laboratory requires sample pretreatment, reagent preparation, and detection and analysis to be distributed in different rooms to prevent contamination.
  • PCR polymerase chain reaction
  • the purpose of the embodiments of the present invention is to provide a detection device and a detection method for nucleic acid extraction and amplification by magnetic bead method.
  • the detection device used for nucleic acid extraction and amplification by magnetic bead method includes cartridges and pipettes.
  • the detection device is used in conjunction with external supporting instruments to automatically and pollution-free realize sample lysis, magnetic suction, and washing of nucleic acid extraction by magnetic bead method. , elution and other steps, and then the amplification and detection of nucleic acid can be completed.
  • the present invention adopts the following technical scheme to realize:
  • a detection device for nucleic acid extraction and amplification by magnetic bead method comprising: a cartridge, wherein the cartridge includes an upper cover, a sealing gasket and an integrated reagent storage tube, the integrated reagent storage tube It includes a plurality of test tubes whose openings are sequentially fixed on the connecting body, and the detection reagents are pre-loaded into each test tube of the integrated reagent storage tube, and then cover the sealing gasket and the upper cover in sequence to realize the sealing of the reagents;
  • the upper cover is provided with a circular opening corresponding to the position of each test tube; and a pipette, which is composed of a needle, a pipette and a filter layer; the rear end of the needle is connected to the pipette, The filter layer is fixed on the rear end of the pipette.
  • the detection device for nucleic acid extraction and amplification by magnetic bead method further includes: a retractable elastic sealing cover, the lower part of the retractable elastic sealing cover is detachably connected with the upper part of the cassette, and the Each of the circular openings is covered in the retractable elastic sealing cover; the pipette is placed in the retractable elastic sealing cover, wherein the rear of the pipette is connected with the retractable elastic sealing cover. The upper part of the elastic sealing cover is sealed and connected.
  • the retractable elastic sealing cover includes an upper interface, a lower interface, and a body; the pipette and the retractable elastic sealing cover are sealedly connected through the upper interface; the upper part of the cartridge is connected to the The retractable elastic sealing cover is connected through the lower interface.
  • the shape of the body is a flat triangular cylindrical shape, a folding triangular cylindrical shape or a accordion triangular cylindrical shape.
  • the triangular shape allows the body to be easily stretched or compressed, thereby facilitating movement of the pipette between individual test tubes of the integrated reagent storage tube to transfer reagents while maintaining a sealed space.
  • the material of the body is an air-impermeable and water-impermeable elastic material, so as to be stretched or compressed and kept isolated from the outside world.
  • the end of the connector of the integrated reagent storage tube is provided with a microfluidic chip, and the part of the microfluidic chip used for fluorescence collection is placed outside the area sealed and covered by the retractable elastic sealing cover.
  • test tubes of the integrated reagent storage tube are arranged in a linear shape, a circular shape or an array shape.
  • connection method includes thread, snap, and tight fit.
  • the pipette further includes a rubber sleeve, the rubber sleeve is wrapped outside the needle, the end of the rubber sleeve is arc-shaped, corresponding to the circular opening and the end of the rubber sleeve is in the shape of an arc. is larger than the diameter of the circular opening.
  • the sealing gasket is in the shape of a flat plate, or preferably, the position of the opening corresponding to each of the test tubes is set in a concave shape.
  • test tubes of the integrated reagent storage tube are in sequence: a magnetic bead tube, a lysis solution tube, a washing solution tube, a washing solution tube one, a washing solution tube two, an eluent tube and a PCR amplification reagent tube. .
  • test tubes of the integrated reagent storage tube are in sequence: : Magnetic beads tube, lysis solution tube, washing solution tube one, washing solution tube two tube one, washing solution tube two tube two, eluent tube and PCR amplification reagent tube.
  • test tubes of the integrated reagent storage tube are as follows: Magnetic beads tube, lysis solution tube, washing solution tube one, washing solution tube two tube one, washing solution tube two tube two and eluent tube.
  • the cartridge, the pipette and the retractable elastic sealing cover are all disposable.
  • a split cover is provided at a position corresponding to the circular opening of the lysate tube, and the split cover penetrates the sealing gasket.
  • the material of the rubber sleeve, the sealing gasket and the split cover is a material that is elastic and can be pierced by the needle.
  • Another aspect of the present invention provides a detection method based on magnetic bead method nucleic acid extraction and amplification, using the detection device for magnetic bead method nucleic acid extraction and amplification provided in the first aspect of the present invention, in advance in an integrated reagent Add detection reagents to each test tube of the storage tube, then add the nucleic acid sample to be tested into the lysis solution tube, seal and install a retractable elastic sealing cover and a pipette, and perform the following steps:
  • the detection device for nucleic acid extraction and amplification by magnetic bead method provided by the present invention has the following beneficial effects:
  • Reagents can be pre-filled, and the detection steps can be automated to realize POCT detection.
  • the order of each test tube is arranged according to the manual operation steps, which is highly coordinated with the movement of the two-dimensional guide rail of the automatic operation device, and can conveniently and quickly realize the extraction and amplification of sample nucleic acid by magnetic bead method. Detection, reduce the manual operation of the detection personnel, improve the detection efficiency and accuracy.
  • the cartridge cover seals the integrated reagent storage tube through a sealing gasket, and the pipetting operation is performed by puncturing the needle of the pipette, and preferably, a retractable elastic sealing cover is used to keep the reagents in the whole detection process. In a closed state, it does not come into contact with the external environment, reduces pollution, expands the applicable scenarios of nucleic acid detection, and facilitates the realization of POCT nucleic acid detection.
  • the integrated reagent storage tube of the embodiment of the present invention reserves the position of the microfluidic chip, which is convenient for the overall upgrading of equipment and testing items.
  • the cartridge and the pipette of the present invention have a wide range of applications and can be expanded to meet various needs. A project for nucleic acid testing.
  • the structure is simple, and it is easy to match with the existing testing facilities.
  • the detection device for nucleic acid extraction and amplification by magnetic bead method according to the embodiment of the present invention has a simple structure, a cartridge and a pipette are integrally arranged, and satisfies the strict requirement that each link of nucleic acid extraction and amplification detection is sealed and free from contamination.
  • the key position, shape and structure of each test tube are the same as the manual operation steps, so it can be matched with the existing detection supporting facilities (such as heating, magnetic suction, fluorescence and other equipment), and can be quickly popularized and applied.
  • Double-layer protection, good sealing For nucleic acid samples with high sealing requirements, a pipette with a rubber sleeve can be used to realize the double-layer protection of the rubber sleeve and the retractable elastic sealing cover when the needle moves between each reagent tube, which ensures the detection process. tightness and safety.
  • FIG. 1 is a schematic structural diagram of a detection device for nucleic acid extraction and amplification by magnetic bead method according to Embodiment 1 of the present invention
  • FIG. 2 is a schematic structural diagram of the upper cover, the sealing gasket, and the integrated reagent storage tube of the cartridge according to the first embodiment of the present invention
  • FIG. 3 is a schematic structural diagram of a pipette (containing a rubber sleeve) according to Embodiment 1 of the present invention
  • FIG. 4 is a schematic diagram of setting a split cover on the cartridge according to Embodiment 1 of the present invention.
  • FIG. 5 is a schematic diagram of the shape and structure of the sealing gasket according to the first embodiment of the present invention.
  • FIG. 6 is a schematic structural diagram of an integrated reagent storage tube with a microfluidic chip according to Embodiment 2 of the present invention.
  • FIG. 7 is a schematic cross-sectional structural diagram of the cartridge, the pipette, the integrated reagent storage tube with a microfluidic chip, and the retractable elastic sealing cover according to the second embodiment of the present invention (with a microfluidic chip).
  • the terms “installed”, “set up”, “provided with”, “connected”, “connected”, “socketed” should be construed broadly. For example, it may be a fixed connection, a detachable connection, or a unitary structure; it may be a mechanical connection, or an electrical connection; it may be directly connected, or indirectly connected through an intermediary, or between two devices, elements, or components. internal communication.
  • installed may be a fixed connection, a detachable connection, or a unitary structure; it may be a mechanical connection, or an electrical connection; it may be directly connected, or indirectly connected through an intermediary, or between two devices, elements, or components. internal communication.
  • Example 1 No microfluidic chip is provided
  • FIG. 1 is a schematic structural diagram of a detection device for nucleic acid extraction and amplification by magnetic bead method according to Embodiment 1 of the present invention.
  • 2 is a schematic structural diagram of an upper cover, a sealing gasket, and an integrated reagent storage tube of the cartridge according to the first embodiment of the present invention.
  • the detection device includes a cartridge, a pipette and a retractable elastic sealing cover 19 .
  • the cartridge includes an upper cover 1 , a sealing gasket 2 , and an integrated reagent storage tube 3 .
  • the integrated reagent storage tube 3 includes seven test tubes 9-15 whose openings are sequentially fixed and connected to the connecting body, and the seven test tubes are arranged in a straight line.
  • the detection reagents are preloaded into each test tube of the integrated reagent storage tube 3, and then covered with the sealing gasket 2 and the upper cover 1, so as to realize the sealing of the reagents in the cartridge.
  • a circular opening 17 is provided on the upper cover 1 at a position corresponding to each test tube.
  • FIG. 3 is a schematic structural diagram of a pipette (containing a rubber sleeve) according to Embodiment 1 of the present invention.
  • the pipette is composed of a needle 5 , a pipette 6 , and a filter layer 7 .
  • the rear end of the needle 5 is connected to the pipette 6, the filter layer 7 is fixed on the rear end of the pipette 6, and the rear end of the pipette 6 is connected with external supporting equipment (such as an air pump) through the adapter 8, and the connection mode is a screw connection.
  • external supporting equipment such as an air pump
  • the pipette further includes a rubber sleeve 4 , which is wrapped outside the needle 5 , and the end of the rubber sleeve 4 is arc-shaped, corresponding to the circular opening 17 and The diameter of the end of the rubber sleeve 4 is larger than the diameter of the circular opening 17 .
  • the lower part of the retractable elastic sealing cover 19 is detachably connected with the upper part of the cartridge, and all the circular openings 17 are covered in the retractable elastic sealing cover 19 .
  • the pipette is placed in a retractable elastic sealing cover 19 , wherein the rear part of the pipette 6 is sealingly connected to the upper part of the retractable elastic sealing cover 19 .
  • the retractable elastic sealing cover 19 is composed of an upper interface 20 , a lower interface 22 and a body 23 .
  • the main body 23 is made of an air-impermeable and water-tight elastic material, such as rubber, so that the main body 23 can not only maintain a good isolation effect, but also be easily stretched and compressed, so as to facilitate the movement of the pipette.
  • the pipette is connected with the retractable elastic sealing cover 19 through the upper port 20 in a sealed manner, and the upper part of the cartridge is connected with the retractable elastic sealing cover 19 through the lower port 22 .
  • the shape of the main body 23 is an organ-type triangular cylinder, so that the main body 23 can be easily stretched or compressed, so as to facilitate the pipette to move between the test tubes of the integrated reagent storage tube 3 to transfer the reagent, and at the same time Keep a sealed space.
  • FIG. 5 is a schematic diagram of the shape and structure of the sealing gasket according to the first embodiment of the present invention.
  • the sealing gasket 2 is in the shape of a recess 18 corresponding to the opening of each test tube.
  • the shape of the recess is convenient for needle piercing and can guide the needle to pierce toward the center.
  • the depression 18 has an upper surface and a lower surface, the lower surface is in a concave shape, the upper surface can be parallel or a concave shape, and it is not required to correspond to the lower surface one-to-one.
  • the material of the rubber sleeve 4 and the sealing gasket 2 is a material that is elastic and can be pierced by a needle, such as rubber, silicone or other polymers.
  • the material of the rubber sleeve 4 and the sealing gasket 2 is rubber.
  • the tip of the needle 5 is an oblique opening, which can pierce through the rubber sleeve 4 and the sealing gasket 2 .
  • the sealing gasket 2 is one or more layers, and the main function is that after the needle 5 passes through the sealing gasket 4 and is pulled out, the perforation on the sealing gasket 4 caused by the needle can be closed by the elasticity of the elastic material itself to prevent contamination.
  • test tubes of the integrated reagent storage tube 3 are sequentially: magnetic bead tube 9, lysate tube 10, washing solution one tube 11, washing solution two tubes one 12, washing solution two tubes two 13, and eluent Tube 14, PCR amplification reagent tube 15.
  • the cartridge, the pipette and the retractable elastic sealing cover 19 are all disposable.
  • FIG. 4 is a schematic diagram of disposing a split cover on the cartridge according to the first embodiment of the present invention.
  • a split cover 21 is provided at a position corresponding to the circular opening 17 of the lysate tube 10 , and the split cover 21 penetrates through the gasket layer.
  • the separate cover 21 can be opened to add the nucleic acid sample to be tested, and then the separate cover 21 can be closed to keep it sealed.
  • the needle 5 of the pipette penetrates the cover of the split cover 21 and enters the lower test tube for operation.
  • the material of the split cover 21 is a material that is elastic and can be pierced by a needle, such as rubber.
  • a Liquid suction When the pipette is inserted vertically downward from the circular opening 17 of the upper cover 1 of the cartridge, the rubber sleeve 4 will first contact the chamfered edge of the circular opening 17 and the upper surface of the sealing gasket 2, and then The rubber sleeve 4 can no longer descend, and the needle 5 wrapped by the rubber sleeve will continue to descend, puncture the rubber sleeve 4 and the gasket 2 in turn, insert it into the corresponding test tube below, turn on the air pump to pump out air, and drive the reagent through the needle 5. Enter pipette 6. Since there is a filter layer 7 between the adapter 8 and the reagent in the pipette, the reagent will not contaminate the adapter 8 and the pipeline and air pump behind it.
  • This embodiment provides a detection method based on magnetic bead method for nucleic acid extraction and nucleic acid amplification, using the above-mentioned detection device for magnetic bead method nucleic acid extraction and amplification, adding in advance to each test tube of the integrated reagent storage tube 3
  • Corresponding reagents specifically, magnetic beads, lysing solution, washing solution 1, washing solution 2, washing solution 2, eluent and nucleic acid amplification reagent are sequentially added to test tubes 9-15 in FIG. 2 .
  • the nucleic acid sample is added to the lysis solution tube 10, the retractable elastic sealing cover 19 and the pipette are sealed and installed, and the detection is carried out with the automatic operation equipment. The following steps are included.
  • the supporting instrument of the adapter 8 is an air pump. After connecting the adapter 8 to the pipette, move it to the top of the lysate tube 10, the pipette moves down, the rubber sleeve 4 touches the circular opening 17, and the needle 5 continues. Down, pierce the rubber sleeve 4 and the sealing gasket 2 in turn, enter the lysate tube 10, the air pump drives the pipette to absorb all the reagents, and then the pipette is lifted upward, and the needle 5 is moved out of the lysate tube 10 and retracted into the rubber sleeve. In 4, the gasket 2 springs back to seal the lysate tube 10. In the following steps, the manner in which the needle 5 passes through the rubber sleeve 4 into the test tube and when the test tube is removed into the rubber sleeve 4 is the same as that in step S1.
  • the integrated reagent storage tube 3 of this embodiment is provided with a PCR amplification reagent tube 15, the pipette is moved to the top of the PCR amplification reagent tube 15, the pipette moves downward, and the needle 5 enters the PCR amplification reagent tube 15,
  • the air pump pushes the gas, drives the pipette to inject the eluate with nucleic acid into the PCR amplification reagent tube 15, controls the air pump to push several times, mixes the liquid, and then lifts the pipette upward, and the needle 5 is removed from the PCR amplification reagent tube 15.
  • Example 2 Microfluidic chips are provided but PCR amplification reagents are not embedded
  • FIG. 6 is a schematic structural diagram of an integrated reagent storage tube with a microfluidic chip according to Embodiment 2 of the present invention. As shown in FIG. 6 , a microfluidic chip 16 is provided at the end of the connector of the integrated reagent storage tube 3 of the detection device for magnetic bead method nucleic acid extraction and amplification provided in this embodiment.
  • the detection device includes a cassette, a pipette and a retractable elastic sealing cover 19 .
  • the cassette includes an upper cover 1 , a sealing gasket 2 , and an integrated reagent storage tube 3 .
  • the integrated reagent storage tube 3 includes The 7 openings are sequentially fixed to the test tubes 9-15 connected to the connecting body, and the 7 test tubes are arranged in a straight line.
  • the detection reagents are preloaded into each test tube of the integrated reagent storage tube 3, and then covered with the sealing gasket 2 and the upper cover 1, so as to realize the sealing of the reagents in the cartridge.
  • a circular opening 17 is provided on the upper cover 1 at a position corresponding to each test tube.
  • the pipette consists of a needle 5, a pipette 6, and a filter layer 7.
  • the back end of the needle 5 is connected to the pipette 6, the filter layer 7 is fixed on the back end of the pipette 6, and the back end of the pipette 6 is connected with external supporting equipment (such as an air pump) through an adapter 8, and the connection method is screw connection.
  • the lower part of the retractable elastic sealing cover 1 is detachably connected with the upper part of the cartridge, and all the circular openings 17 are covered in the retractable elastic sealing cover 19 .
  • the pipette is placed in a retractable elastic sealing cover 19 , wherein the rear part of the pipette 6 is sealingly connected to the upper part of the retractable elastic sealing cover 19 .
  • the retractable elastic sealing cover 19 is composed of an upper interface 20 , a lower interface 22 and a body 23 .
  • the body 23 is made of air and water impermeable rubber.
  • the pipette is connected with the retractable elastic sealing cover 19 through the upper port 20 in a sealed manner, and the upper part of the cartridge is connected with the retractable elastic sealing cover 19 through the lower port 22 .
  • the shape of the body 23 is an organ-type triangular cylindrical shape.
  • a microfluidic chip 16 is provided at the end of the connector of the integrated reagent storage tube 3 , and the part of the microfluidic chip 16 used for fluorescence collection is placed in a retractable elastic sealing cover 19 to seal outside the covered area.
  • the microfluidic chip 16 is not pre-embedded with PCR amplification reagents. Therefore, the test tubes of the integrated reagent storage tube 3 are: magnetic bead tube 9, lysate tube 10, washing solution tube 1 11, washing solution tube 2 12, washing solution tube 2 13, eluent tube 14, PCR amplification tube 1 Add reagent tube 15.
  • the end of the connector of the integrated reagent storage tube 3 can be provided with a microfluidic chip 16.
  • the integrated reagent storage tube 3 retains the PCR amplification reagent tube 15;
  • the integrated reagent storage tube 3 does not need to retain the PCR amplification reagent tube 15, and the extracted nucleic acid sample is directly put into the microfluidic chip for amplification detection.
  • the gasket 2 is in the shape of a flat plate.
  • the rubber sleeve 4 and the sealing gasket 2 are made of rubber that is elastic and can be pierced by a needle.
  • the cartridge, the pipette and the retractable elastic sealing cover 19 are all disposable.
  • This embodiment provides a detection method based on magnetic bead method for nucleic acid extraction and nucleic acid amplification, using the above-mentioned detection device for magnetic bead method nucleic acid extraction and amplification, adding in advance to each test tube of the integrated reagent storage tube 3 Corresponding reagents, and then the nucleic acid sample is added to the lysate tube 10 .
  • the matching machine of adapter 8 is an air pump. After connecting adapter 8 to the pipette, move it to the top of the lysate tube 10, the pipette moves down, the needle 5 continues downward, pierces the sealing gasket 2, and enters the lysate tube. 10. The air pump drives the pipette to absorb all the reagents, then the pipette is lifted up, the needle 5 is removed from the lysate tube 10, and the sealing gasket 2 rebounds to seal the lysate tube 10.
  • the detection device for nucleic acid extraction and amplification by the magnetic bead method according to the embodiment of the present invention can be used in conjunction with the matching automatic detection equipment, or the detection personnel can hold a pipette and operate it manually on the cassette, which can be achieved.
  • Fast and efficient detection compared with conventional PCR laboratory testing, can significantly shorten the detection time and greatly improve the detection efficiency.
  • the sample since it is ensured that the whole process of operation is sealed, the sample will not leak and will not be contaminated, so it can be operated in an ordinary laboratory without the need for a high-standard PCR laboratory to meet the needs of POCT.

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Abstract

Appareil de détection pour un procédé à billes magnétiques pour l'extraction et l'amplification d'acides nucléiques, et procédé de détection basé sur le procédé à billes magnétiques pour l'extraction et l'amplification d'acides nucléiques. L'appareil de détection comprend une cartouche et un dispositif de pipette. La cartouche comprend un couvercle supérieur (1), un joint d'étanchéité (2) et un tube de stockage de réactif intégré (3) ; le tube de stockage de réactif intégré (3) comprend une pluralité de tubes à essai (9 à 15) dont les ouvertures sont fixées et connectées successivement sur un corps de connexion, un réactif de détection est préchargé dans chaque tube à essai du tube de stockage de réactif intégré (3), puis le joint d'étanchéité (2) et le couvercle supérieur (1) sont recouverts successivement pour réaliser l'étanchéité du réactif ; et des ouvertures circulaires (17) sont prévues sur le couvercle supérieur (1) correspondant aux positions des tubes à essai. Le dispositif de pipette se compose d'une aiguille (5), d'une pipette (6) et d'une couche filtrante (7) ; une extrémité arrière de l'aiguille (5) est reliée à la pipette (6), et la couche filtrante (7) est fixée à une extrémité arrière de la pipette (6).
PCT/CN2022/085077 2021-04-06 2022-04-02 Appareil de détection et procédé de détection de billes magnétiques pour l'extraction et l'amplification d'acides nucléiques WO2022213930A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN202120689520.3 2021-04-06
CN202120689520.3U CN216378192U (zh) 2021-04-06 2021-04-06 一种用于磁珠法核酸提取与核酸扩增的检测装置
CN202110365658.2A CN115181662A (zh) 2021-04-06 2021-04-06 一种用于磁珠法检测的密封式一体化装置及其应用
CN202110365658.2 2021-04-06

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