WO2022211319A1 - Dispositif de détection de bactéries de type à polarisation de concentration d'ions et procédé de détection de bactéries l'utilisant - Google Patents

Dispositif de détection de bactéries de type à polarisation de concentration d'ions et procédé de détection de bactéries l'utilisant Download PDF

Info

Publication number
WO2022211319A1
WO2022211319A1 PCT/KR2022/003661 KR2022003661W WO2022211319A1 WO 2022211319 A1 WO2022211319 A1 WO 2022211319A1 KR 2022003661 W KR2022003661 W KR 2022003661W WO 2022211319 A1 WO2022211319 A1 WO 2022211319A1
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
ion
electrode
channel
ion concentration
Prior art date
Application number
PCT/KR2022/003661
Other languages
English (en)
Korean (ko)
Inventor
김성재
김원석
박재석
Original Assignee
서울대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 서울대학교산학협력단 filed Critical 서울대학교산학협력단
Publication of WO2022211319A1 publication Critical patent/WO2022211319A1/fr

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria

Definitions

  • the present invention relates to an ion concentration polarization type bacteria detection apparatus and a method for detecting bacteria using the same, and more particularly, by using the ion concentration polarization phenomenon to concentrate particles and simultaneously dissolve the concentrated particles in a temperature range where an enzyme reaction is promoted. It relates to an ion concentration polarization type bacteria detection device and a bacteria detection method using the same.
  • Particulate matter has a great impact on the human body and the global environment through various forms and routes. With the development of related industries and increasing interest in the environment, its importance is also gradually emerging. Molecules that affect us, from detecting specific particles that can obtain biometric information in living fluids, for example, bacteria in urine, to finding low-concentration environmental substances distributed in the air, depend on their size. and different concentrations. In order to efficiently measure and analyze this, various sensors and reactors have been developed.
  • a pre-treatment process of concentration to a desired level by these sensors and reactors is performed, and then a post-processing process for the concentrated one is performed.
  • the post-processing process needs to be performed by connecting separate devices for processing and analyzing particles in a multi-stage form, and this type has a complicated configuration and takes a lot of time and money to detect a specific particle. There is this.
  • An object of the present invention is to solve various problems including the above problems, and an object of the present invention is to provide an ion concentration polarization type bacteria detection device capable of simultaneously dissolving bacteria in the device and a bacteria detection method using the same.
  • these problems are exemplary, and the scope of the present invention is not limited thereto.
  • a channel unit including a first channel having an inlet into which a fluid is injected and a second channel connected to the first channel and having an outlet through which the fluid is discharged; an ion permeable membrane layer disposed on the channel portion to provide an ion transfer path; and an electrode unit including a first electrode to which a negative voltage is applied; Including, but the ion concentration polarization (ICP; Ion Concentration Polarization) phenomenon occurs in the vicinity of the branch point between the channel part and the ion permeable membrane layer, the bacteria from the fluid passing through the channel part is concentrated, the first electrode is the ion
  • ICP Ion Concentration Polarization
  • An ion concentration polarization type bacteria detection device may be provided which is disposed closely on one side of the permeable membrane layer and is provided to generate alkaline water by electrolyzing water in the fluid at a portion adjacent to the first electrode.
  • the alkaline water may dissolve the bacteria concentrated by the ion concentration polarization phenomenon.
  • the patch unit may store a dye sample for dyeing the bacteria dissolved by the alkaline water.
  • the channel part may include cellulose paper.
  • the pores inside the cellulose paper may have a size of 1 um to 100 um.
  • the electrode unit may further include a second electrode to which a ground or positive voltage is applied.
  • the method comprising: applying a negative voltage to the first electrode; injecting a fluid containing bacteria into the inlet; electrolyzing water in the fluid to generate alkaline water in an area adjacent to the first electrode; Concentrating the bacteria from the fluid by forming an ion depletion zone (ICP Zone) by generating the ion concentration polarization (ICP) phenomenon in a region adjacent to a branch point between the channel part and the ion permeable membrane layer; and dissolving the concentrated bacteria in the alkaline water; including, a method for detecting bacteria may be provided.
  • ICP Zone ion depletion zone
  • ICP ion concentration polarization
  • Dyeing the bacteria dissolved by the alkaline water with a dye sample stored in the patch, detecting the presence of the bacteria may further include.
  • an ion concentration polarization type bacteria detection apparatus capable of detecting a specific bacteria within a short time and a bacteria detection method using the same.
  • the scope of the present invention is not limited by these effects.
  • FIG. 1 is a diagram schematically illustrating an ion concentration polarization type bacteria detection apparatus according to an embodiment of the present invention.
  • Figure 4 (a) is the result of observing the color change in the picture shown by the detection of bacteria over time
  • Figure 4 (b) is the color change according to the presence or absence of Ion Concentration Polarization (ICP) phenomenon It is a graph showing the ratio according to the bacterial concentration
  • FIG. 5 is a step-by-step diagram illustrating a bacteria detection method using an ion concentration polarization type bacteria detection apparatus according to an embodiment of the present invention.
  • FIG. 1 is a diagram schematically illustrating an ion concentration polarization type bacteria detection apparatus 1000 according to an embodiment of the present invention.
  • the ion concentration polarization type bacteria detection apparatus 1000 includes a first channel 100 having an inlet into which a fluid is injected, and an outlet connected to the first channel 100 through which the fluid is discharged.
  • a channel portion having a second channel 200 provided therewith, an ion permeable membrane layer 500 disposed on the channel portion, a first electrode 10 to which a negative voltage is applied, and a second electrode to which a ground or positive voltage is applied ( 20) may be included.
  • An inlet into which the fluid is injected may be provided at one end of the first channel 100 , and an outlet through which the fluid remaining after passing through the channel is discharged may be provided at the other end of the second channel 200 .
  • the first channel 100 and the second channel 200 are connected and constitute one channel unit.
  • the ion permeable membrane layer 500 is disposed on the upper portion of the channel portion to provide a transfer path of ions in the fluid passing through the channel portion. That is, the ion concentration polarization type bacteria detection apparatus 1000 according to an embodiment of the present invention exists in the form of a single fluid tube rather than a structure in which the channel part is separated, so that ions present in the fluid when the fluid passes through the channel part It allows it to pass through the ion permeable membrane layer 500 at this high speed.
  • ICP ion concentration polarization
  • an ion concentration polarization (ICP) phenomenon occurs in an area adjacent to the branch point between the channel part and the ion permeable membrane layer 500 to form an ion depletion zone (ICP Zone), as described above
  • Bacteria may be concentrated from the fluid at a nearby point in the area.
  • the fluid may be understood as an arbitrary charged solution, for example, may include urine containing bacteria.
  • alkaline water is generated by electrolysis of water in the fluid passing through the channel in the adjacent portion of the first electrode 10 , and the first electrode 10 is disposed in close contact with the ion permeable membrane layer 500 .
  • Alkaline water generated from the first electrode 10 may dissolve bacteria concentrated in the region near the ion permeable membrane layer 500 .
  • the ion concentration polarization type bacteria detection apparatus 1000 may further include a patch unit 300 disposed adjacent to the first electrode 10 .
  • the patch unit 300 stores a staining sample 300a for staining bacteria dissolved by alkaline water. More specifically, when bacteria are dissolved by alkaline water, a specific enzyme is ejected, and the above-described staining sample 300a is provided to dye the ejected specific enzyme. That is, the presence or absence of specific bacteria in the fluid may be detected through the patch unit 300 .
  • the dye sample 300a may be, for example, a nitrocefin solution.
  • the periphery of the channel part may be coated with wax. Accordingly, it is possible to minimize the loss of fluid by suppressing a phenomenon in which water in the fluid passing through the channel part leaks around the channel part.
  • the channel portion may include cellulose paper.
  • cellulose paper due to the porous structure of the cellulose paper, electric convection phenomenon can be suppressed when an electric field is applied to the channel portion, and electrical instability can be minimized during ion concentration polarization.
  • the porous structure of the above-described cellulose paper enables the channel portion to have a high electrotreatment capacity, and thus the channel portion may exhibit stable ion transport efficiency even under a high voltage electric field.
  • the pores inside the cellulose paper may have a size of, for example, 1 um to 100 um.
  • the channel unit of the ion concentration polarization type bacteria detection apparatus 1000 may include, for example, cellulose paper having a porous structure having a pore size distribution of 1 ⁇ m to 100 ⁇ m.
  • the above-described porous structure performs the role of parallelized microchannels, it is possible to concentrate a large-capacity solution at the level of a body fluid.
  • the electrode unit includes a first electrode 10 to which a negative voltage is applied and a second electrode 20 to which a ground or positive voltage is applied.
  • the second electrode 20 may be provided as a positive (+) electrode due to a potential difference with the first electrode 10 .
  • the first electrode 10 is disposed closely on one side of the ion-permeable membrane layer 500 . That is, since the bacteria that have passed through the ion permeable membrane layer 500 are concentrated in the vicinity of the ion permeable membrane layer 500 , the corresponding concentration point and the first electrode 10 may also be disposed adjacent to each other. Accordingly, the bacteria may be concentrated by the ion concentration polarization phenomenon and, at the same time, dissolved by the alkaline water generated by the first electrode 10 .
  • FIG. 2 in a paper device in which a sample for measuring a change in pH is stored, for example, under each voltage applied to 140V, 160V, 180V, and 200V, the color of the sample gradually increases at the point where the bacteria are concentrated. It can be confirmed that it becomes darker, and as shown in the graph, this means that the pH value of the corresponding point is gradually increasing.
  • the ion concentration polarization type bacteria detection apparatus 1000 can quickly detect a specific bacteria in a fluid, and the detection can be performed through the patch unit 300 .
  • the above-described bacterial dissolution phenomenon requires that the temperature conditions for promoting the enzymatic reaction, for example, temperature conditions of 35 °C to 40 °C be satisfied.
  • the temperature conditions for promoting the enzymatic reaction for example, temperature conditions of 35 °C to 40 °C be satisfied.
  • FIG. 3 in the paper device in which the sample for measuring the temperature change is stored, it can be seen that the color of the sample gradually fades at the point where the bacteria are concentrated under a constant voltage. As shown in the graph, this means that the temperature of the corresponding point is gradually increasing.
  • a constant voltage for example, a voltage of 140 V is applied to the electrode unit. It can be confirmed that a predetermined temperature condition in which the enzymatic reaction is promoted can be achieved only by electrical resistance.
  • a channel was printed on nitrocellulose paper (Whatman filter paper num. 1, Sigma-Aldrich, USA) and heated at 130° C. for 3 minutes to constitute one channel part inside the paper.
  • Paraffin film (Parafilm M) was bonded above and below the channel to prevent external contamination and evaporation of the internal fluid.
  • the ion-permeable membrane layer 500 made of a nanoporous membrane sheet (Sigma-Aldrich, USA), an electrode part made of Ag, and a Nitrocefin (Sigma-Aldrich, USA) solution as a dyeing sample (300a) coated patch part (300)
  • urine was injected into the inlet part provided at one end of the first channel 100 of the channel part as a fluid passing through the channel part. Then, after 380 seconds (180 seconds of sample injection + 200 seconds of device operation) after urine was injected using a mobile phone, color changes were photographed, and the photographed pictures were analyzed.
  • Figure 4 (a) is the result of observing the color change in the photograph shown by the detection of bacteria over time
  • Figure 4 (b) is a photograph taken according to the presence or absence of Ion Concentration Polarization (ICP) phenomenon Among them, it is a graph showing the ratio of the color change according to the bacterial concentration.
  • ICP Ion Concentration Polarization
  • Bacteria concentrated by the ion concentration polarization (ICP) phenomenon may be dissolved by the alkaline water generated from the first electrode 10 at the same time as the concentration.
  • a specific enzyme is ejected, and the dye sample 300a stored in the patch part 300 stains the specific enzyme, so that the color change appears in the patch part 300 .
  • FIG. 4( a ) it can be seen that the color of the patch part 300 is changed within 3 minutes even when bacteria exist only at a low concentration level of about 10 4 cfu/mL.
  • the above-described bacteria are simply separated from the fluid by the ion concentration polarization (ICP) phenomenon and go through concentration, and the ion concentration polarization type bacteria detection device 1000 according to an embodiment of the present invention Even if the lysed bacteria are present only in low concentrations, the detection of the bacteria may be possible.
  • ICP ion concentration polarization
  • the ion concentration polarization type bacteria detection apparatus 1000 may be provided, for example, in the form of an automated small device.
  • FIG. 5 is a diagram illustrating a method for detecting bacteria according to an embodiment of the present invention.
  • Ion concentration polarization (ICP) Polarization may include a step of concentrating bacteria from the fluid by a phenomenon, dissolving the concentrated bacteria with alkaline water, and detecting the presence of bacteria through a patch part.
  • ICP ion concentration polarization
  • Ion concentration polarization (ICP) phenomenon is one of the electrochemical transfer phenomena observed around structures having nano-membrane.
  • the nano-membrane may be understood as the ion-permeable membrane layer 500 of the present invention.
  • the thickness of the electric double layer is similar to the size of the wall distance within the nanomembrane, the electric double layer overlaps inside the nanomembrane to show single ion permeability. Ions having the same type of charge as the wall charge of the nanomembrane do not pass through the nanomembrane due to diffusion and drift force, but only ions having the opposite type of charge to the wall charge pass through, and excessive depletion of ions at the nanomembrane interface phenomenon appears.
  • a strong electrical repulsive force acts between the ions that do not pass through the nano-membrane, so that both cations and anions are affected, and thus an ion concentration gradient appears.
  • charged bacteria, cells, and droplets are also affected by the electrical repulsive force of ions at the interface of the ion depletion zone (ICP Zone) and are pushed out around the nano-membrane.
  • the material may be electrically balanced by the force due to electroosmosis and the force due to electrophoresis, and the material may be concentrated at the equilibrium point of the force located near the ion depletion zone (ICP Zone).
  • the equilibrium point may be different depending on the size and charge amount of the molecular weight of each material.
  • the step of concentrating the bacteria by the above-described ion concentration polarization (ICP) phenomenon and the step of dissolving the bacteria may be simultaneously performed.
  • ICP ion concentration polarization
  • applying a negative voltage to the first electrode 10 and electrolyzing water in the fluid supplied to the inlet of the first channel 100 constituting the channel portion to the first electrode The step of generating alkaline water in the adjacent part of (10) is preceded, which is a process that proceeds continuously and automatically when a voltage is applied to the electrode.
  • alkaline water including hydroxide ions (OH - ) is generated through the electrolysis reaction of water (H 2 O) in the fluid, and the concentrated bacteria are in the already generated alkaline water. It can be dissolved simultaneously with concentration. Accordingly, the presence or absence of a specific bacteria can be detected within a short time through the method for detecting bacteria according to an embodiment of the present invention.
  • the bacteria dissolved by the alkaline water spout a specific enzyme, and the staining sample 300a stored in the patch unit 300 stains the specific enzyme spewed from the bacteria to detect the presence of the corresponding bacteria. Since the step of detecting the presence or absence of bacteria targets bacteria concentrated due to the ion concentration polarization phenomenon, the method for detecting bacteria according to an embodiment of the present invention enables detection of the bacteria even in the presence of only low-concentration bacteria. do.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un dispositif de détection de bactéries de type à polarisation de concentration d'ions (ICP) et un procédé de détection de bactéries l'utilisant, le dispositif comprenant : une unité de canal comprenant un premier canal ayant une partie d'entrée à travers laquelle un fluide est injecté et un second canal ayant une partie de sortie reliée au premier canal pour évacuer le fluide à travers celle-ci ; une couche de membrane perméable aux ions disposée sur l'unité de canal pour fournir un trajet de transfert d'ions ; et une unité d'électrode comprenant une première électrode à laquelle une tension négative est appliquée, l'ICP se produisant dans une zone adjacente à un point de ramification entre l'unité de canal et la couche de membrane perméable aux ions. Ainsi, les bactéries sont concentrées à partir du fluide traversant l'unité de canal, la première électrode est disposée à proximité d'un côté de la couche de membrane perméable aux ions et disposée de telle sorte que l'eau dans le fluide est électrolysée dans une partie adjacente de la première électrode pour générer de l'eau alcaline.
PCT/KR2022/003661 2021-04-02 2022-03-16 Dispositif de détection de bactéries de type à polarisation de concentration d'ions et procédé de détection de bactéries l'utilisant WO2022211319A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020210043482A KR102489135B1 (ko) 2021-04-02 2021-04-02 이온농도분극형 박테리아 검출장치 및 이를 이용한 박테리아 검출방법
KR10-2021-0043482 2021-04-02

Publications (1)

Publication Number Publication Date
WO2022211319A1 true WO2022211319A1 (fr) 2022-10-06

Family

ID=83457652

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2022/003661 WO2022211319A1 (fr) 2021-04-02 2022-03-16 Dispositif de détection de bactéries de type à polarisation de concentration d'ions et procédé de détection de bactéries l'utilisant

Country Status (2)

Country Link
KR (1) KR102489135B1 (fr)
WO (1) WO2022211319A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101754845B1 (ko) * 2016-03-17 2017-07-19 한국과학기술연구원 동전기적 현상의 위치 제어를 이용한 농축시스템
KR20190129403A (ko) * 2018-05-11 2019-11-20 포항공과대학교 산학협력단 세포의 용해 및 세포내 성분의 분리를 위한 이온 농도 분극 기반의 다기능 미세 유체 장치
JP2019536066A (ja) * 2016-11-18 2019-12-12 カルス インコーポレイテッド ラテラルフロー型アッセイストリップ用の濃縮キット
KR20200074832A (ko) * 2018-12-17 2020-06-25 광운대학교 산학협력단 마이크로채널내의 생체 시료 속도 및 위치제어를 통한 샘플 농축 및 분리 장치

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101754845B1 (ko) * 2016-03-17 2017-07-19 한국과학기술연구원 동전기적 현상의 위치 제어를 이용한 농축시스템
JP2019536066A (ja) * 2016-11-18 2019-12-12 カルス インコーポレイテッド ラテラルフロー型アッセイストリップ用の濃縮キット
KR20190129403A (ko) * 2018-05-11 2019-11-20 포항공과대학교 산학협력단 세포의 용해 및 세포내 성분의 분리를 위한 이온 농도 분극 기반의 다기능 미세 유체 장치
KR20200074832A (ko) * 2018-12-17 2020-06-25 광운대학교 산학협력단 마이크로채널내의 생체 시료 속도 및 위치제어를 통한 샘플 농축 및 분리 장치

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DAS ARINDAM K., MANOHAR MURLI, SHAHI VINOD K.: "Cation-Exchange Membrane with Low Frictional Coefficient and High Limiting Current Density for Energy-Efficient Water Desalination", ACS OMEGA, ACS PUBLICATIONS, US, vol. 3, no. 8, 31 August 2018 (2018-08-31), US , pages 10331 - 10340, XP055972804, ISSN: 2470-1343, DOI: 10.1021/acsomega.8b01403 *

Also Published As

Publication number Publication date
KR20220137417A (ko) 2022-10-12
KR102489135B1 (ko) 2023-01-17

Similar Documents

Publication Publication Date Title
Kaya et al. A novel electro-driven membrane for removal of chromium ions using polymer inclusion membrane under constant DC electric current
Kinosita et al. Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope
Restan et al. Comprehensive study of buffer systems and local pH effects in electromembrane extraction
CA1335805C (fr) Procede d'electrofocalisation et appareil servant a sa mise en oeuvre
CN109569458A (zh) 用于形成含极性介质的体积的阵列的装置
Ramos-Payán Liquid-Phase microextraction and electromembrane extraction in millifluidic devices: A tutorial
JPH04264253A (ja) 膜上での毛細電気泳動留分の収集のための装置
KR101511569B1 (ko) 입자 분리 장치
ATE473040T1 (de) Multi-port-elektrophorese-trennvorrichtung und entsprechendes verfahren
Benavente et al. Effect of adsorbed protein on the hydraulic permeability, membrane and streaming potential values measured across a microporous membrane
WO2022211319A1 (fr) Dispositif de détection de bactéries de type à polarisation de concentration d'ions et procédé de détection de bactéries l'utilisant
Dong et al. Unidirectional solute transfer using a Janus membrane
Yamamoto et al. On-line microchip electrophoresis-mediated preconcentration of cationic compounds utilizing cationic polyacrylamide gels fabricated by in situ photopolymerization
Lee et al. Folding-paper-based preconcentrator for low dispersion of preconcentration plug
Koryta Electrochemistry of liquid membranes: interfacial aspects
Khajeh et al. Extraction of caffeine and gallic acid from coffee by electrokinetic methods coupled with a hollow‐fiber membrane
CN207248814U (zh) 一种纳米孔生物标记物检测微流池
CN113340945B (zh) 一种萃取膜及渗透汽化膜结构与性能的电化学检测方法
CN103938367A (zh) 一种以apam为阳极膜的纳米纤维双极膜的制备方法
WO2018182082A1 (fr) Focalisation isoélectrique basée sur une puce microfluidique fondée sur une détection de conductivité sans contact et ne nécessitant aucune pompe
Kobayashi et al. Toward a molecular Coulter® counter type device
Thompson et al. Bilayer lipid membrane electrochemistry in a flow injection system
Schanke et al. Transport among protocells via tunneling nanotubes
EP1449292A2 (fr) Electrode a membrane generatrice de champ
KR20000010983A (ko) 액체 크로마토그래피용 전기화학검출기, 액체 크로마토그래피장치 및 이 장치를 이용한 분석방법

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 22781439

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 22781439

Country of ref document: EP

Kind code of ref document: A1