WO2022128756A1 - Method for detecting adsorption differences, accumulation and/or retention regions in partially translucent containers - Google Patents

Method for detecting adsorption differences, accumulation and/or retention regions in partially translucent containers Download PDF

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Publication number
WO2022128756A1
WO2022128756A1 PCT/EP2021/085113 EP2021085113W WO2022128756A1 WO 2022128756 A1 WO2022128756 A1 WO 2022128756A1 EP 2021085113 W EP2021085113 W EP 2021085113W WO 2022128756 A1 WO2022128756 A1 WO 2022128756A1
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Prior art keywords
container
dye
liquid
light
differences
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PCT/EP2021/085113
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German (de)
French (fr)
Inventor
Tomas Pink
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Tomas Pink
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Priority claimed from EP21180197.2A external-priority patent/EP4016056A1/en
Application filed by Tomas Pink filed Critical Tomas Pink
Publication of WO2022128756A1 publication Critical patent/WO2022128756A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6447—Fluorescence; Phosphorescence by visual observation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/88—Investigating the presence of flaws or contamination
    • G01N21/91—Investigating the presence of flaws or contamination using penetration of dyes, e.g. fluorescent ink
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N2021/646—Detecting fluorescent inhomogeneities at a position, e.g. for detecting defects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N2021/8411—Application to online plant, process monitoring
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/88—Investigating the presence of flaws or contamination
    • G01N21/90—Investigating the presence of flaws or contamination in a container or its contents
    • G01N21/9018—Dirt detection in containers
    • G01N21/9027—Dirt detection in containers in containers after filling

Definitions

  • the object of the invention is to make adsorption differences, accumulation and/or retention areas in at least partially translucent containers recognizable, in particular from outside the containers.
  • the object is achieved in particular by a method for detecting differences in adsorption, accumulation and/or retention areas in containers, in which a liquid containing a dye dissolved therein is introduced into a container, characterized in that the container has an at least partially translucent (r/s ) and/or transparent plastic reactor, non-metallic fluid system, single-use assembly and/or single-use structure and wherein the container with the liquid contained therein is exposed, in particular from the outside, to light, in particular UV light, in particular UV-A light, is irradiated and the differences in the optical view of the container containing the liquid resulting from the different distribution of the dye inside the container are viewed from the outside, analyzed and/or recorded becomes.
  • the liquid is preferably neither drained nor removed before the observation, analysis and/or recording and/or the container is not rinsed either.
  • the object is also achieved by using a dissolved dye in an at least partially translucent and/or transparent container in order to detect differences in adsorption, accumulation and/or to reveal retention areas of the dye and/or an associated molecule, wherein the container is a plastic reactor, non-metallic fluid system, disposable assembly and/or construction.
  • Elastomers such as silicones and TPE (thermoplastic elastomers) such as C-Flex, Advantaflex, Pure Weid and/or
  • Composite materials such as fiberglass composites and/or blends, laminates or layers of elastomers and plastic.
  • Translucent and/or transparent means in particular a high light transmission, in particular of at least 30%, preferably at least 50%, in the and/or over the wavelength range(s) in which the color and/or fluorescence of the dye and in in and/or above the wavelength(s) of the light used for irradiation and/or in and/or above the visible and/or UV-A range.
  • the UV-A range includes in particular the wavelength range from 380 to 315 nm.
  • the entire container does not have to be translucent and/or transparent. For example, in man- Chen be arranged non-transparent functional means.
  • the container is designed in such a way that at least 20%, in particular 50% to 100%, in particular 100%, of the areas of the wall of the container that come into contact with the liquid and/or the areas of the container that come into contact with the liquid are brought into contact, can be viewed from the outside through transparent and/or translucent sections of the container and/or that at least 20%, in particular at least 50%, of the wall of the container which is brought into contact with the liquid is transparent and/or are designed to be translucent. Viewing, analysis and/or recording from the outside and, in particular, irradiation from the outside make it easy and safe to carry out and also enable testing of areas in which irradiation and/or viewing from the inside is not possible or only possible with difficulty.
  • the irradiation is advantageously carried out in such a way that the container and/or the liquid contained therein is irradiated completely, at the same time or with a time delay.
  • areas with a dye concentration that is higher than the environment or the agent, in particular that exceeds a predetermined limit value are identified.
  • the brightness of the light emitted and/or reflected by the dye increases continuously and strictly monotonically with the dye concentration.
  • areas with compared to the environment or the center of the Container increased, in particular exceeding a predetermined limit value, identified the brightness of the emitted and / or reflected light from the dye. This allows the advantages of the method to be used particularly well.
  • the liquid is advantageously a penetrant solution and/or the dye is a fluorescent dye, in particular a fluorophore or chromophore, and/or the liquid and/or the dye is/are thixotropic. This allows a particularly good detection to be achieved.
  • Riboflavin for example, can be used as a dye.
  • light with a wavelength of 360 nm is used for the irradiation and the transmission is in particular 70% and more, in particular at 360 and at 550 nm.
  • Fluorescein can easily be covalently coupled to different biomolecules, which is preferred for many applications.
  • light with a wavelength of 480 to 500 nm is used for the irradiation and the transmission is, in particular, 70% and more, in particular at 520 to 530 nm.
  • the dye is advantageously bound to at least one molecule, in particular an organic molecule, in particular a biomolecule, in particular covalently, and/or it is a conjugate. This enables the adsorption differences, attachment and/or retention areas of the at least one molecule to be considered.
  • the container is filled, in particular completely, with the liquid during the observation, analysis and/or recording.
  • the liquid during the observation, analysis and/or recording.
  • water and/or alcohols and/or a liquid containing water and/or alcohol can be used for rinsing.
  • fluorescein as the dye, for example, it has proven advantageous to start with a water/alcohol mixture and slowly increase the alcohol concentration during the rinsing.
  • it can also be rinsed, for example, with the agent and/or a process buffer subsequently used in the normal production process.
  • the rinsing takes place in particular by pumping a rinsing liquid, in particular a process buffer, liquid containing water and/or alcohol through the container.
  • the container is particularly advantageously a thermoplastic and/or elastomeric container, in particular a hose and/or reaction vessel, in particular made of a thermoplastic elastomer. This is where the process has proven to be particularly useful.
  • the observation, analysis and/or recording preferably takes place under irradiation with polarized light.
  • This increases the contrast and in particular allows mechanical stresses and/or damage caused thereby, such as cracks, to be recognized particularly clearly.
  • the liquid is advantageously pumped through the container, in particular at least during the observation, analysis and/or recording under irradiation with the light. As a result, differences in adsorption, accumulation and/or retention areas in all areas and the effect of flow can be identified.
  • the container is a “single-use” container, in particular a “single-use assembly”, in particular one that is used to produce a substance.
  • the wall of the container that is brought into contact with the liquid and/or the container is made of plastic, in particular completely.
  • single-use material is understood to mean a material or a mixture of materials which, in the case of disposal, in particular dumping and/or incineration, in particular in the case of incineration at temperatures in the range of 800-1 150 °C, in particular 800 °C and 900 °C, and/or in the case of fluidized bed combustion and/or grate combustion, do not release any dangerous by-products or emissions and either burn without residue and/or decompose or only leave residues which are harmless to People and the environment are and/or those that meet the requirements of the BImSchG, the BImSchV and/or Industrial Emissions Directive 2010/75/EU (Integrated Pollution Prevention and Control) in the latest and/or valid version at the time of registration, especially in the case of combustion at temperatures in the range of 800-1 150 °C, in particular 800 °C and 900 °C, and/or in the case of fluidized bed firing and/or grate firing.
  • BImSchG the BImSchV and/or Industrial Emission
  • plastics preferably do not contain any rare elements or other valuable materials, in particular no materials whose value is reduced by more than 50% due to the effect of heat, in particular no permanent magnets. It is preferably plastic, enamel, ceramic, steel, tungsten carbide, titanium nitride, aluminum oxide, metal and semimetal nitrides, metal carbides, metal and semimetal oxides, glass and/or carbon composite material, glass and/or carbon fibers , Aramid, aramid resin and/or aluminum and aluminum alloys, magnesium alloys, preferably plastic.
  • Polypropylene, polyethylene, polyvinyl acetate, polyethyl vinyl acetate, PEEK, PET, polyamides and polyimides can be considered as the plastic, for example.
  • the process allows not only very small cracks in the range of 10-20 /m, which do not even have to go through the entire wall thickness, to be clearly and easily detected. Cavities and dead legs ("dead legs”) as well as mechanically overloaded areas can also be visualized. As a result, processes in the container can be made safer and/or the purity and/or yield of the process taking place in the container can be increased. For this reason, the process is also suitable for quality control and for product testing and product development.

Abstract

The present invention relates to detecting adsorption differences, accumulation and/or retention regions in partially translucent containers, in particular "single-use assemblies". The aim of the invention is to make adsorption differences, accumulation and/or retention regions in partially translucent containers visible, in particular from the outside of the containers. This aim is achieved in particular by a method for detecting adsorption differences, accumulation and/or retention regions in containers, in which method a liquid containing a dissolved dye is introduced into a container, characterized in that the container is an at least partially translucent and/or transparent plastic reactor, non-metal fluid system, single-use assembly and/or single-use structure, and in which method the container, including the liquid contained therein, is irradiated, in particular from the outside, with light, in particular UV light, in particular UV-A light, and the differences in the visual appearance of the liquid-containing container, which differences result from the different distribution of the dye in the interior of the container, are inspected, analyzed and/or recorded from the outside.

Description

Verfahren zur Erkennung von Adsorptionsunterschieden, Anlagerungs- und/oder Rückhaltbereichen in teilweise lichtdurchlässigen Behältnissen Method for detecting differences in adsorption, accumulation and/or retention areas in partially transparent containers
Die vorliegende Erfindung befasst sich mit der Erkennung von Adsorptionsunterschieden, Anlagerungs- und/oder Rückhaltbereichen in teilweise lichtdurchlässigen Behältnissen, insbesondere „Single-Use Assemblies". The present invention deals with the detection of differences in adsorption, accumulation and/or retention areas in partially translucent containers, in particular “single-use assemblies”.
Es ist aus der US 3,965,350 A bekannt, fluoreszierende Farben zur Erkennung von Rissen, vor allem in Metallen, zu nutzen. Auch ist es bekannt, dies unter polarisiertem Licht oder bei Betrachtung durch Polfilter durchzuführen, insbesondere bezogen auf Teile von Turbinen oder Flügeln so aus der US 10,054,552 und US 5,554,318 A oder bezogen auf Metalle im Allgemeinen aus der WO 00/603,44 A1 oder der JP 2015 094 642 A. It is known from US Pat. No. 3,965,350 A to use fluorescent colors to identify cracks, especially in metals. It is also known to do this under polarized light or when viewed through polarizing filters, in particular with regard to parts of turbines or wings from US Pat. Nos. 10,054,552 and US Pat JP 2015 094 642 A
Auch ist eine Anwendung zur Erkennung von Rissen in Zähnen aus der US 4,204,978 A und in Keramikbauteilen aus der WO 2000/047,982 A1 und in Membranen aus der WO 2013/1 10,458 A1 bekannt. Auch ist die Nutzung zur Erkennung von Spannungen unter polarisiertem Licht und bei Betrachtung durch Polfilter aus der US 2006/192,177 A1 bekannt. Auch ist es aus der US 4,400,618 bekannt, fluoreszierende Farben, die ausgehärtet werden, zu nutzen, um Fehler in Schnitten durch Platinen zu erkennen. Auch lassen sich, wie aus der WO 1990/000,268 A1 bekannt ist, Schäden an metallischen Rohren unter Wasser durch polarisiertes Licht erkennen. Ebenfalls bekannt ist die Verwendung fluoreszierender Bestandteile in einer Zubereitung zur Beschichtung, um deren An- Wesenheit zu kontrollieren aus der CN 1 10862747 A. Auch werden fluoreszierende Kontrastmittel zur Untersuchung von Geweben unter Nutzung von Polfiltern in der WO 2005/027,730 A2 genutzt. An application for detecting cracks in teeth is also known from US Pat. No. 4,204,978 A and in ceramic components from WO 2000/047,982 A1 and in membranes from WO 2013/110,458 A1. Also known from US 2006/192,177 A1 is the use for detecting voltages under polarized light and when viewed through polarizing filters. It is also known from US Pat. No. 4,400,618 to use fluorescent inks that are cured to detect defects in cuts through circuit boards. As is known from WO 1990/000,268 A1, damage to metallic pipes under water can also be detected by polarized light. Also known is the use of fluorescent components in a preparation for coating in order to Being to be checked from CN 1 10862747 A. Fluorescent contrast media are also used to examine tissues using polarizing filters in WO 2005/027,730 A2.
Aus der JP 3 141389 U ist eine Referenzprobe bekannt, die dadurch gebildet wird, dass ein an sich transparentes Gefäß mit einer intransparenten Schicht versehen wird, in die eine Fehlstelle eingebracht wird und das Behältnis mit UV-fluo- reszierendem Farbstoff gefüllt und mit UV-Licht bestrahlt wird. A reference sample is known from JP 3 141389 U, which is formed in that a transparent container is provided with a non-transparent layer into which a defect is introduced and the container is filled with UV-fluorescent dye and filled with UV light is irradiated.
Aus der WO 2013/144215 A1 ist es bekannt zum Training von Qualitätskontrollen durchführenden Mitarbeitern, Stellen mangelhafter Qualität, beispielsweise in Glasampullen, mit UV-fluoreszierendem Farbstoff zu kennzeichnen. From WO 2013/144215 A1 it is known for the training of employees who carry out quality controls to mark places of poor quality, for example in glass ampoules, with UV-fluorescent dye.
Aufgabe der Erfindung ist es, Adsorptionsunterschiede, Anlagerungs- und/oder Rückhaltbereiche in zumindest teilweise lichtdurchlässigen Behältnissen erkennbar zu machen, insbesondere von außerhalb der Behältnisse. The object of the invention is to make adsorption differences, accumulation and/or retention areas in at least partially translucent containers recognizable, in particular from outside the containers.
Gelöst wird die Aufgabe durch die Nutzung einer einen Farbstoff enthaltenden Flüssigkeit, die mit Licht bestrahlt wird und die Beobachtung der sich durch die unterschiedliche Verteilung des Farbstoffs im Inneren des Behältnis ergebenen Unterschiede in der optischen Ansicht des die Flüssigkeit beinhaltenden Behältnisses von außen. The task is solved by using a liquid containing a dye, which is irradiated with light and by observing the differences in the optical view of the container containing the liquid from the outside, which result from the different distribution of the dye inside the container.
Gelöst wird die Aufgabe insbesondere durch ein Verfahren zur Erkennung von Adsorptionsunterschieden, Anlagerungs- und/oder Rückhaltbereichen in Behältnissen, wobei eine einen darin gelösten Farbstoff enthaltende Flüssigkeit in ein Behältnis eingeführt wird, dadurch gekennzeichnet, dass das Behältnis ein zumindest teilweise lichtdurchlässige(r/s) und/oder transparente(r/s) Kunststoffreaktor, nichtmetallisches Fluidsystem, Einweg-Baugruppe und/oder Einwegaufbau ist und wobei das Behältnis mit darin enthaltender Flüssigkeit, insbesondere von außen, mit Licht, insbesondere UV-Licht, insbesondere UV-A Licht, bestrahlt wird und die sich durch die unterschiedliche Verteilung des Farbstoffs im Inneren des Behältnis ergebenen Unterschiede in der optischen Ansicht des die Flüssigkeit beinhaltenden Behältnisses von außen betrachtet, analysiert und/oder aufgenommen wird. Dabei wird bevorzugt die Flüssigkeit vor der Betrachtung, Analyse und/oder Aufnahme weder abgelassen, noch entfernt und/oder das Behältnis auch nicht gespült. The object is achieved in particular by a method for detecting differences in adsorption, accumulation and/or retention areas in containers, in which a liquid containing a dye dissolved therein is introduced into a container, characterized in that the container has an at least partially translucent (r/s ) and/or transparent plastic reactor, non-metallic fluid system, single-use assembly and/or single-use structure and wherein the container with the liquid contained therein is exposed, in particular from the outside, to light, in particular UV light, in particular UV-A light, is irradiated and the differences in the optical view of the container containing the liquid resulting from the different distribution of the dye inside the container are viewed from the outside, analyzed and/or recorded becomes. The liquid is preferably neither drained nor removed before the observation, analysis and/or recording and/or the container is not rinsed either.
Gelöst wird die Aufgabe auch durch Verwendung eines gelösten Farbstoffs in einem zumindest teilweise lichtdurchlässigen und/oder transparenten Behältnis, um von außen unter Bestrahlung von Licht, insbesondere UV-Licht, insbesondere UV-A Licht, zur Erkennung von Adsorptionsunterschieden, Anlagerungs- und/oder Rückhaltbereichen des Farbstoffs und/oder eines damit verbundenen Moleküls erkennbar zu machen, wobei das Behältnis ein Kunststoffreaktor, nichtmetallisches Fluidsystem, eine Einweg-Baugruppe und/oder ein Einwegaufbau ist. The object is also achieved by using a dissolved dye in an at least partially translucent and/or transparent container in order to detect differences in adsorption, accumulation and/or to reveal retention areas of the dye and/or an associated molecule, wherein the container is a plastic reactor, non-metallic fluid system, disposable assembly and/or construction.
Vorteilhafterweise handelt es sich bei dem Behältnis um ein Behältnis aus The container is advantageously made of a container
Plastik wie PP, PE, PS (Polystyrene), PA (Polyamide), PC (Polycarbonate), PMMA, PVAC (Polyvinylacetate), PVOH (Polyvinylalcohol), EVA (Ethylvinylace- tate), PTFE, und/oder PFA, und/oder Plastics such as PP, PE, PS (polystyrene), PA (polyamide), PC (polycarbonate), PMMA, PVAC (polyvinyl acetate), PVOH (polyvinyl alcohol), EVA (ethyl vinyl acetate), PTFE, and/or PFA, and/or
Elastomer wie Silikone und TPE (Thermoplastischen Elastomere), wie C-Flex, Ad- vantaflex, Pure Weid und/oder Elastomers such as silicones and TPE (thermoplastic elastomers) such as C-Flex, Advantaflex, Pure Weid and/or
Kompositmaterialien wie z.B. Glasfaserkomposite und/oder oder Gemische, Laminate oder Schichtungen von Elastomeren und Plastik. Composite materials such as fiberglass composites and/or blends, laminates or layers of elastomers and plastic.
Lichtdurchlässige und/oder transparent bedeutet insbesondere eine hohe Lichttransmission, insbesondere von mindestens 30%, bevorzugt mindestens 50%, in den/dem und/oder über den/die Wellenlängenbereich(en), in denen die Farbe und/oder Fluoreszenz des Farbstoffs und in den/dem und/oder über den/die Wel- lenlänge(n) des zum Bestrahlen genutzten Lichts liegt und/oder im und/oder über den sichtbaren und/oder UV-A Bereich. UV-A Bereich umfasst dabei insbesondere den Wellenlängenbereich von 380 bis 315 nm. Insbesondere ist die hohe Lichttransmission, insbesondere mindestens 30%, bevorzugt mindestens 50%, von 315 bis 650 nm gegeben. Dabei muss nicht das gesamte Behältnis lichtdurchlässig und/oder transparent gebildet sein. So können zum Beispiel in man- chen Bereichen nicht transparente funktionale Mittel angeordnet sein. Insbesondere ist das Behältnis so beschaffen, dass mindestens 20%, insbesondere 50% bis 100%, insbesondere 100%, der Bereiche der Wandung des Behältnisses, die mit der Flüssigkeit in Berührung gebracht wird, und/oder der Bereiche des Behältnisses, die mit der Flüssigkeit in Berührung gebracht werden, durch transparente und/oder lichtdurchlässige Abschnitte des Behältnisses von außen betrachtbar sind und/oder dass mindestens 20%, insbesondere mindestens 50%, der Wandung des Behältnisses, die mit der Flüssigkeit in Berührung gebracht wird, transparent und/oder lichtdurchlässig ausgebildet sind. Die Betrachtung, Analyse und/oder Aufnahme von außen und insbesondere auch die Bestrahlung von außen, machen die Durchführung einfach, sicher und ermöglichen die Prüfung auch von Bereichen, in denen eine Bestrahlung und/oder Betrachtung von innen nicht oder nur schwer möglich ist. Translucent and/or transparent means in particular a high light transmission, in particular of at least 30%, preferably at least 50%, in the and/or over the wavelength range(s) in which the color and/or fluorescence of the dye and in in and/or above the wavelength(s) of the light used for irradiation and/or in and/or above the visible and/or UV-A range. The UV-A range includes in particular the wavelength range from 380 to 315 nm. In particular, there is high light transmission, in particular at least 30%, preferably at least 50%, from 315 to 650 nm. The entire container does not have to be translucent and/or transparent. For example, in man- Chen be arranged non-transparent functional means. In particular, the container is designed in such a way that at least 20%, in particular 50% to 100%, in particular 100%, of the areas of the wall of the container that come into contact with the liquid and/or the areas of the container that come into contact with the liquid are brought into contact, can be viewed from the outside through transparent and/or translucent sections of the container and/or that at least 20%, in particular at least 50%, of the wall of the container which is brought into contact with the liquid is transparent and/or are designed to be translucent. Viewing, analysis and/or recording from the outside and, in particular, irradiation from the outside make it easy and safe to carry out and also enable testing of areas in which irradiation and/or viewing from the inside is not possible or only possible with difficulty.
Besonders vorteilhaft ist die Anwendung des Verfahrens und/oder die Verwendung somit insbesondere an Behältnissen, die mindestens einen Abschnitt aufweisen, der einen freien Innendurchmesser von weniger als 50 mm, insbesondere weniger als 10 mm aufweist und dieser Abschnitt mit der Flüssigkeit in Berührung gebracht wird, bestrahlt wird und betrachtet, analysiert und/oder aufgenommen wird. It is particularly advantageous to apply the method and/or use it in particular on containers that have at least one section with a free inner diameter of less than 50 mm, in particular less than 10 mm, and this section is brought into contact with the liquid, is irradiated and viewed, analyzed and/or recorded.
Dadurch das der Farbstoff gelöst eingebracht wird, wird eine ausreichende, insbesondere ausreichend homogene Verteilung in der Flüssigkeit und/oder dem Behältnis gewährleistet. Because the dye is introduced in dissolved form, sufficient, in particular sufficiently homogeneous, distribution in the liquid and/or the container is ensured.
Mit Vorteil erfolgt die Bestrahlung so, dass das Behältnis und/oder die darin enthaltene Flüssigkeit vollständig, zeitgleich oder zeitversetzt, bestrahlt wird. The irradiation is advantageously carried out in such a way that the container and/or the liquid contained therein is irradiated completely, at the same time or with a time delay.
Mit Vorteil werden mittels der Betrachtung, Analyse und/oder Aufnahme Bereiche mit gegenüber der Umgebung oder dem Mittel erhöhter, insbesondere einen vorbestimmten Grenzwert übersteigender, Farbstoffkonzentration identifiziert. Dabei wird insbesondere davon ausgegangen, dass die Helligkeit des vom Farbstoff emittierten und/oder reflektierten Lichts stetig mit der Farbstoffkonzentration und streng monoton steigt. Mit Vorteil werden somit mittels der Betrachtung, Analyse und/oder Aufnahme Bereiche mit gegenüber der Umgebung oder dem Mittel des Behältnisses erhöhter, insbesondere einen vorbestimmten Grenzwert übersteigender, Helligkeit des vom Farbstoff emittierten und/oder reflektierten Lichts identifiziert. Dadurch lassen sich die Vorteile des Verfahrens besonders gut nutzen. Advantageously, by means of the observation, analysis and/or recording, areas with a dye concentration that is higher than the environment or the agent, in particular that exceeds a predetermined limit value, are identified. In this case, it is assumed in particular that the brightness of the light emitted and/or reflected by the dye increases continuously and strictly monotonically with the dye concentration. Advantageously, by means of the observation, analysis and/or recording, areas with compared to the environment or the center of the Container increased, in particular exceeding a predetermined limit value, identified the brightness of the emitted and / or reflected light from the dye. This allows the advantages of the method to be used particularly well.
Mit Vorteil ist die Flüssigkeit eine Penetrantlösung und/oder ist der Farbstoff ein fluoreszierender Farbstoff, insbesondere ein Fluoro- oder Chromophor, ist und/oder ist/sind die Flüssigkeit und/oder der Farbstoff thixotrop. Dadurch lässt sich eine besonders gute Erkennung erreichen. The liquid is advantageously a penetrant solution and/or the dye is a fluorescent dye, in particular a fluorophore or chromophore, and/or the liquid and/or the dye is/are thixotropic. This allows a particularly good detection to be achieved.
Als Farbstoff kann beispielsweise Riboflavin verwendet werden. Dabei wird zur Bestrahlung insbesondere Licht mit einer Wellenlänge von 360 nm verwendet und ist die Transmission, von insbesondere 70% und mehr, insbesondere bei 360 und bei 550 nm gegeben. Riboflavin, for example, can be used as a dye. In particular, light with a wavelength of 360 nm is used for the irradiation and the transmission is in particular 70% and more, in particular at 360 and at 550 nm.
Ein weiteres Beispiel ist Fluoreszein. Fluoreszein kann einfach mit unterschiedlichen Biomolekülen kovalent gekoppelt werden, was für zahlreiche Anwendungen bevorzugt ist. Dabei wird zur Bestrahlung insbesondere Licht mit einer Wellenlänge von 480 bis 500 nm verwendet und ist die Transmission, von insbesondere 70% und mehr, insbesondere bei 520 bis 530 nm gegeben. Another example is fluorescein. Fluorescein can easily be covalently coupled to different biomolecules, which is preferred for many applications. In particular, light with a wavelength of 480 to 500 nm is used for the irradiation and the transmission is, in particular, 70% and more, in particular at 520 to 530 nm.
Mit Vorteil ist der Farbstoff an mindestens ein Molekül, insbesondere organisches Molekül, insbesondere Biomolekül, insbesondere kovalent, gebunden und/oder ist er ein Konjugat. Dies ermöglicht die Betrachtung der Adsorptionsunterschieden, Anlagerungs- und/oder Rückhaltbereichen des mindestens einen Moleküls. The dye is advantageously bound to at least one molecule, in particular an organic molecule, in particular a biomolecule, in particular covalently, and/or it is a conjugate. This enables the adsorption differences, attachment and/or retention areas of the at least one molecule to be considered.
In manchen Anwendungen wird es bevorzugt, wenn das Behältnis bei der Betrachtung, Analyse und/oder Aufnahme, insbesondere vollständig, mit der Flüssigkeit gefüllt ist. Dadurch lassen sich Adsorptionsunterschieden, Anlagerungs- und/oder Rückhaltbereichen in allen Bereichen erkennen. In some applications it is preferred if the container is filled, in particular completely, with the liquid during the observation, analysis and/or recording. As a result, adsorption differences, accumulation and/or retention areas can be recognized in all areas.
In manchen Anwendungsfällen wird es bevorzugt, alternativ oder insbesondere anschließend an eine erste Betrachtung, Analyse und/oder Aufnahme und/oder während der Betrachtung, Analyse und/oder Aufnahme, insbesondere unter Bestrahlung mit dem Licht, insbesondere dem selben Licht, eine Spülung des Behältnisses und/der ein Ablaufen und/der ein Abpumpen der Flüssigkeit aus dem Behältnis durchgeführt wird und/oder nach der Spülung, dem Ablauf und/oder dem Abpumpen eine weitere Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit Licht, insbesondere dem selben Licht, durchgeführt wird. Dadurch lassen sich die Adsorptionsunterschiede, Anlagerungs- und/oder Rückhaltbereiche unter/oder nach der Spülung erkennen. Zur Spülung kann beispielsweise Wasser und/oder Alkohole und/oder eine Wasser und/oder Alkohol enthaltende Flüssigkeit verwendet werden. Beispielsweise bei Fluoreszein als Farbstoff hat es sich als vorteilhaft erwiesen, mit einem Wasser/Alkoholgemisch zu starten und die Alkoholkonzentration während der Spülung langsam zu steigern. Es kann aber beispielsweise auch mit dem im normalen Herstellungsprozess anschließend verwendeten Mittel und/oder einem Prozessbuffer gespült werden. Das Spülen erfolgt insbesondere durch Durchpumpen einer Spülflüssigkeit, insbesondere eines Prozessbuffers, Wasser und/oder Alkohol enthaltenden Flüssigkeit, durch das Behältnis. In some applications it is preferred, alternatively or in particular subsequent to a first observation, analysis and/or recording and/or during the observation, analysis and/or recording, in particular under irradiation with the light, in particular the same light, to rinse the container and / of a drain and / of a pumping of the liquid from the Container is carried out and / or after the rinsing, the drain and / or the pumping, a further observation, analysis and / or recording under irradiation with light, in particular the same light, is carried out. As a result, the differences in adsorption, accumulation and/or retention areas under/or after the flushing can be identified. For example, water and/or alcohols and/or a liquid containing water and/or alcohol can be used for rinsing. With fluorescein as the dye, for example, it has proven advantageous to start with a water/alcohol mixture and slowly increase the alcohol concentration during the rinsing. However, it can also be rinsed, for example, with the agent and/or a process buffer subsequently used in the normal production process. The rinsing takes place in particular by pumping a rinsing liquid, in particular a process buffer, liquid containing water and/or alcohol through the container.
Mit besonderem Vorteil handelt es sich bei dem Behältnis um ein thermoplastisches und/oder elastomeres Behältnis, insbesondere Schlauch und/oder Reaktionsgefäß, insbesondere aus thermoplastischem Elastomer. Hier hat sich das Verfahren als besonders zielführend erwiesen. The container is particularly advantageously a thermoplastic and/or elastomeric container, in particular a hose and/or reaction vessel, in particular made of a thermoplastic elastomer. This is where the process has proven to be particularly useful.
Bevorzugt erfolgt die Betrachtung, Analyse und/oder Aufnahme hinter einem Polfilter, insbesondere Interferenzfilter, insbesondere linear oder zirkular. Dies erhöht den Kontrast. Mit Vorteil kann die Betrachtung, Analyse und/oder Aufnahme mit unterschiedlichen und/oder unterschiedlich orientiertem Polfilter erfolgen. Dadurch lassen sich einige Adsorptionsunterschiede, Anlagerungs- und/oder Rückhaltbereiche besonders deutlich erkennen. The observation, analysis and/or recording preferably takes place behind a polarizing filter, in particular an interference filter, in particular linear or circular. This increases the contrast. Advantageously, the observation, analysis and/or recording can take place with different and/or differently oriented polarizing filters. As a result, some adsorption differences, accumulation and/or retention areas can be recognized particularly clearly.
Bevorzugt erfolgt die Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit polarisiertem Licht. Dies erhöht den Kontrast und lässt insbesondere mechanische Beanspruchungen und/oder dadurch entstandene Beschädigungen wie Risse besonders deutlich erkennen. Mit Vorteil kann die Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit unterschiedlich polarisiertem, insbe- sondere linear oder zirkular, und/oder mit unterschiedlich orientiertem polarisiertem Licht erfolgen. Dadurch lassen sich einige Adsorptionsunterschiede, Anlage- rungs- und/oder Rückhaltbereiche besonders deutlich erkennen. The observation, analysis and/or recording preferably takes place under irradiation with polarized light. This increases the contrast and in particular allows mechanical stresses and/or damage caused thereby, such as cracks, to be recognized particularly clearly. Advantageously, the observation, analysis and/or recording under irradiation with differently polarized, in particular special linear or circular, and / or take place with differently oriented polarized light. As a result, some differences in adsorption, accumulation and/or retention areas can be recognized particularly clearly.
Mit Vorteil wird die Flüssigkeit durch das Behältnis gepumpt, insbesondere zumindest auch während der Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit dem Licht. Dadurch lassen sich Adsorptionsunterschiede, Anlage- rungs- und/oder Rückhaltbereiche in allen Bereichen und die Auswirkung von Strömung erkennen. The liquid is advantageously pumped through the container, in particular at least during the observation, analysis and/or recording under irradiation with the light. As a result, differences in adsorption, accumulation and/or retention areas in all areas and the effect of flow can be identified.
Mit besonderem Vorteil wird, ein chemisches und/oder pharmakologisches und/oder biologisches Verfahren, insbesondere Herstellungsverfahren, unter Anwesenheit der Flüssigkeit durchgeführt und während dessen dauerhaft und/oder wiederholt, mindestens zwei mal, insbesondere mehr als zehn mal, insbesondere jeweils in einem zeitlichen Abstand im Bereich von 1 ms bis 30 Tagen, die Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit dem Licht durchgeführt. Insbesondere wird der Farbstoff und/der ein daran gebundenes Molekül im Behältnis während der Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit dem Licht und/oder zwischen der wiederholten Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit dem Licht chemisch und/oder biologisch umgesetzt und/oder verbraucht. Dadurch lassen sich Adsorptionsunterschiede, Anlage- rungs- und/oder Rückhaltbereiche während des Prozesses erkennen. With particular advantage, a chemical and/or pharmacological and/or biological process, in particular a manufacturing process, is carried out in the presence of the liquid and during this is carried out continuously and/or repeatedly, at least twice, in particular more than ten times, in particular at a time interval in the range of 1 ms to 30 days, the observation, analysis and/or recording is performed under irradiation with the light. In particular, the dye and/or a molecule bound thereto becomes chemical and/or biological in the container during viewing, analysis and/or recording under irradiation with the light and/or between repeated viewing, analysis and/or recording under irradiation with the light implemented and/or consumed. As a result, differences in adsorption, accumulation and/or retention areas can be identified during the process.
Insbesondere handelt es sich bei dem Behältnis um ein „Single-Use" Behältnis, insbesondere um eine „Single-Use Assembly", insbesondere eine solche die zur Herstellung einer Substanz verwendet wird. In particular, the container is a “single-use” container, in particular a “single-use assembly”, in particular one that is used to produce a substance.
Insbesondere ist die mit der Flüssigkeit in Berührung gebrachte Wandung des Behältnisses und/oder das Behältnis, insbesondere vollständig, aus Kunststoff gebildet. In particular, the wall of the container that is brought into contact with the liquid and/or the container is made of plastic, in particular completely.
Unter „Single-Use"-Material wird in dieser Schrift ein Material oder eine Materialmischung verstanden, die bei einer Entsorgung, insbesondere Deponierung und/oder Verbrennung, insbesondere bei Verbrennung bei Temperaturen im Bereich von 800-1 150 °C, insbesondere 800 °C und 900 °C, und/oder bei der Wirbelschichtfeuerung und/oder Rostfeuerung, keine gefährlichen Nebenprodukte oder Emissionen freigeben und entweder Rückstandslos verbrennen und/oder zerfallen oder nur Rückstände hinterlassen, die unbedenklich für Mensch und Umwelt sind und/oder solche die die Anforderungen der BImSchG, der BImSchV und/oder Industrial Emissions Directive 2010/75/EU (Integrated Pollution Prevention and Control) in der zum Anmeldezeitpunkt letzten und/oder gültigen Fassung, insbesondere bei Verbrennung bei Temperaturen im Bereich von 800-1 150 °C, insbesondere 800 °C und 900 °C, und/oder bei der Wirbelschichtfeuerung und/oder Rostfeuerung, erfüllen. Bevorzugt beinhalten sie keine seltene Elemente oder anders wertvolle Materialien, insbesondere keine Materialien, deren Wert durch die Hitzeeinwirkung um mehr als 50% reduziert wird, insbesondere keine Permanentmagnete. Bevorzugt handelt es sich um Kunststoff, Emaille, Keramik, Stahl, Wolframkarbid, Titannitrid, Aluminiumoxid, Metall-und Halbmetall-Nitride, Metall- Carbide, Metall-und Halbmetall-Oxide, Glas- und/oder Kohlenstoffverbundwerkstoff, Glas- und/oder Kohlenstofffasern, Aramid, Aramidharz und/oder Aluminium und Aluminiumlegierungen, Magnesiumlegierungen, bevorzugt um Kunststoff. Als Kunststoff kommt beispielsweise in Betracht Polypropylen, Polyethylen, Polyvinylacetat, Polyethylvinylacetat, PEEK, PET, Polyamide und Polyimide. In this document, “single-use” material is understood to mean a material or a mixture of materials which, in the case of disposal, in particular dumping and/or incineration, in particular in the case of incineration at temperatures in the range of 800-1 150 °C, in particular 800 °C and 900 °C, and/or in the case of fluidized bed combustion and/or grate combustion, do not release any dangerous by-products or emissions and either burn without residue and/or decompose or only leave residues which are harmless to People and the environment are and/or those that meet the requirements of the BImSchG, the BImSchV and/or Industrial Emissions Directive 2010/75/EU (Integrated Pollution Prevention and Control) in the latest and/or valid version at the time of registration, especially in the case of combustion at temperatures in the range of 800-1 150 °C, in particular 800 °C and 900 °C, and/or in the case of fluidized bed firing and/or grate firing. They preferably do not contain any rare elements or other valuable materials, in particular no materials whose value is reduced by more than 50% due to the effect of heat, in particular no permanent magnets. It is preferably plastic, enamel, ceramic, steel, tungsten carbide, titanium nitride, aluminum oxide, metal and semimetal nitrides, metal carbides, metal and semimetal oxides, glass and/or carbon composite material, glass and/or carbon fibers , Aramid, aramid resin and/or aluminum and aluminum alloys, magnesium alloys, preferably plastic. Polypropylene, polyethylene, polyvinyl acetate, polyethyl vinyl acetate, PEEK, PET, polyamides and polyimides can be considered as the plastic, for example.
Durch das Verfahren lassen sich nicht nur sehr kleine Risse auch im Bereich von 10-20 /m, die nicht einmal durch die gesamte Wandstärke hindurch gehen müssen, eindeutig und einfach erkennen. Auch lassen sich Kavitäten und Totraumvolumen ("Dead Legs") sowie mechanisch überbelastete Stellen sichtbar machen. Dadurch lassen sich Prozesse in dem Behältnis sicherer gestalten und/oder die Reinheit und/oder Ausbeute des im Behältnis ablaufenden Prozesses erhöhen. Aus diesem Grund eignet sich das Verfahren auch zur Qualitätskontrolle und für Produkttests und die Produktenwicklung. The process allows not only very small cracks in the range of 10-20 /m, which do not even have to go through the entire wall thickness, to be clearly and easily detected. Cavities and dead legs ("dead legs") as well as mechanically overloaded areas can also be visualized. As a result, processes in the container can be made safer and/or the purity and/or yield of the process taking place in the container can be increased. For this reason, the process is also suitable for quality control and for product testing and product development.

Claims

9 Ansprüche 9 claims
1 . Verfahren zur Erkennung von Adsorptionsunterschieden, Anlagerungs- und/oder Rückhaltbereichen in Behältnissen, wobei eine einen Farbstoff enthaltende Flüssigkeit in ein Behältnis eingeführt wird, wobei das Behältnis ein zumindest teilweise lichtdurchlässige(r/s) und/oder transparente(r/s) Kunststoffreaktor, nichtmetallisches Fluidsystem, Einweg-Baugruppe und/oder Einwegaufbau ist und wobei das Behältnis mit darin enthaltender Flüssigkeit, insbesondere von außen, mit Licht, insbesondere UV-Licht, insbesondere UV-A Licht, bestrahlt wird, dadurch gekennzeichnet, dass die sich durch die unterschiedliche Verteilung des Farbstoffs im Inneren des Behältnis ergebenen Unterschiede in der optischen Ansicht des Flüssigkeit beinhaltenden Behältnisses von außen betrachtet, analysiert und/oder aufgenommen werden und der Farbstoff in der Flüssigkeit gelöst in das Behältnis eingeführt wird. 1 . Method for detecting differences in adsorption, accumulation and/or retention areas in containers, in which a liquid containing a dye is introduced into a container, in which the container is an at least partially translucent and/or transparent plastic reactor, non-metallic fluid system, single-use assembly and/or single-use structure, and wherein the container with the liquid it contains is irradiated, in particular from the outside, with light, in particular UV light, in particular UV-A light, characterized in that the different Distribution of the dye inside the container resulting differences in the optical view of the liquid containing container viewed from the outside, analyzed and / or recorded and the dye dissolved in the liquid is introduced into the container.
2. Verfahren nach Anspruch 1 , wobei mittels der Betrachtung, Analyse und/oder Aufnahme Bereiche mit gegenüber der Umgebung oder dem Mittel erhöhter, insbesondere einen vorbestimmten Grenzwert übersteigender, Farbstoffkonzentration identifiziert werden. 2. The method as claimed in claim 1, wherein areas with a dye concentration which is higher than the environment or the agent, in particular exceeding a predetermined limit value, are identified by means of the observation, analysis and/or recording.
3. Verfahren nach einem der vorstehenden Ansprüche, wobei die Flüssigkeit eine Penetrantlösung ist und/oder der Farbstoff ein fluoreszierender Farbstoff, insbesondere ein Fluoro- oder Chromophor, ist und/oder wobei die Flüssigkeit und/oder der Farbstoff thixotrop ist. 3. The method according to any one of the preceding claims, wherein the liquid is a penetrant solution and/or the dye is a fluorescent dye, in particular a fluorophore or chromophore, and/or wherein the liquid and/or the dye is thixotropic.
4. Verfahren nach einem der vorstehenden Ansprüche, wobei der Farbstoff an mindestens ein Molekül, insbesondere organisches Molekül, insbesondere Biomolekül, insbesondere kovalent, gebunden ist und/oder ein Konjugat ist. 4. The method according to any one of the preceding claims, wherein the dye is bound to at least one molecule, in particular organic molecule, in particular biomolecule, in particular covalently, and/or is a conjugate.
5. Verfahren nach einem der vorstehenden Ansprüche, wobei das Behältnis bei der Betrachtung, Analyse und/oder Aufnahme, insbesondere vollständig, mit der Flüssigkeit gefüllt ist. Verfahren nach einem der vorstehenden Ansprüche, wobei während der Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit dem Licht eine Spülung des Behältnisses und/der ein Ablaufen und/der ein Abpumpen der Flüssigkeit aus dem Behältnis durchgeführt wird und/oder nach der Spülung, dem Ablauf und/oder dem Abpumpen eine weitere Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit Licht, insbesondere dem selben Licht, durchgeführt wird. Verfahren nach einem der vorstehenden Ansprüche, wobei es sich bei dem Behältnis um ein thermoplastisches und/oder elastomeres Behältnis handelt. Verfahren nach einem der vorstehenden Ansprüche, wobei die Betrachtung, Analyse und/oder Aufnahme hinter einem Polfilter, insbesondere Interferenzfilter, erfolgt und oder das Licht polarisiert ist. Verfahren nach einem der vorstehenden Ansprüche, wobei die Flüssigkeit durch das Behältnis gepumpt wird, insbesondere zumindest auch während der Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit dem Licht. Verfahren nach einem der vorstehenden Ansprüche, wobei ein chemisches und/oder pharmakologisches und/oder biologisches Verfahren, insbesondere Herstellungsverfahren, unter Anwesenheit der Flüssigkeit durchgeführt wird und während dessen dauerhaft und/oder wiederholt die Betrachtung, Analyse und/oder Aufnahme unter Bestrahlung mit dem Licht durchgeführt wird. Verwendung eines Farbstoffes in einem zumindest teilweise lichtdurchlässigen und/oder transparenten Behältnis, um unter Bestrahlung von Licht Adsorptionsunterschiede, Anlagerungs- und/oder Rückhaltbereiche des Farbstoffs und/oder eines damit verbundenen Moleküls durch die sich durch die unterschiedliche Verteilung des Farbstoffs im Inneren des Behältnis ergebenen Unterschiede in der optischen Ansicht des die Flüssigkeit beinhaltenden Behältnisses von 11 außen erkennbar zu machen, wobei das Behältnis ein Kunststoffreaktor, nichtmetallisches Fluidsystem, eine Einweg-Baugruppe und/oder ein Einwegaufbau ist. Verwendung nach Anspruch 1 1 , wobei das Behältnis gespült wird, um von außen unter Bestrahlung von Licht die Erkennung von Adsorptionsunterschieden des Farbstoffes und/oder des damit verbundenen Moleküls erkennbar zu machen. Verwendung nach einem der Anspruch 1 1 bis 12, wobei der Farbstoff in einer Flüssigkeit enthalten ist und insbesondere die Flüssigkeit eine Penetrantlösung ist und/oder der Farbstoff ein fluoreszierender Farbstoff, insbesondere ein Fluoro- oder Chromophor, ist und/oder wobei die Flüssigkeit und/oder der Farbstoff thixotrop ist und/oder wobei der Farbstoff an mindestens ein Molekül, insbesondere organisches Molekül, insbesondere Biomolekül, insbesondere kovalent, gebunden ist und/oder ein Konjugat ist. Verwendung nach einem der Anspruch 1 1 bis 13, wobei mindestens ein Polfilter zum erkennbar machen verwendet wird. 5. The method as claimed in one of the preceding claims, in which the container is filled, in particular completely, with the liquid during the observation, analysis and/or recording. Method according to one of the preceding claims, wherein during the observation, analysis and/or recording under irradiation with the light, the container is rinsed and/or the liquid is drained and/or pumped out of the container and/or after the rinsing, the process and/or the pumping out, a further observation, analysis and/or recording is carried out under irradiation with light, in particular the same light. A method according to any one of the preceding claims, wherein the container is a thermoplastic and/or elastomeric container. Method according to one of the preceding claims, in which the observation, analysis and/or recording takes place behind a polarizing filter, in particular an interference filter, and/or the light is polarized. Method according to one of the preceding claims, wherein the liquid is pumped through the container, in particular at least also during the observation, analysis and/or recording under irradiation with the light. Method according to one of the preceding claims, wherein a chemical and/or pharmacological and/or biological method, in particular a manufacturing method, is carried out in the presence of the liquid and during which the observation, analysis and/or recording under irradiation with the light is carried out continuously and/or repeatedly is carried out. Use of a dye in an at least partially translucent and/or transparent container in order, under irradiation of light, to determine differences in adsorption, accumulation and/or retention areas of the dye and/or a molecule connected thereto as a result of the different distribution of the dye inside the container Differences in the optical view of the container containing the liquid from 11, the container being a plastic reactor, non-metallic fluid system, disposable assembly and/or assembly. Use according to claim 11, wherein the container is rinsed in order to make the detection of differences in adsorption of the dye and/or the molecule connected thereto recognizable from the outside under irradiation with light. Use according to one of Claims 1 1 to 12, in which the dye is contained in a liquid and in particular the liquid is a penetrant solution and/or the dye is a fluorescent dye, in particular a fluorophore or chromophore, and/or the liquid and/or or the dye is thixotropic and/or wherein the dye is bound to at least one molecule, in particular organic molecule, in particular biomolecule, in particular covalently, and/or is a conjugate. Use according to one of claims 1 1 to 13, wherein at least one polarizing filter is used to make it recognizable.
PCT/EP2021/085113 2020-12-16 2021-12-09 Method for detecting adsorption differences, accumulation and/or retention regions in partially translucent containers WO2022128756A1 (en)

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Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3417241A (en) * 1965-12-07 1968-12-17 Ronald E. Davis Empty bottle inspection method and apparatus utilizing both water soluble and oil soluble fluorescent dyes
US3965350A (en) 1972-01-24 1976-06-22 Air Products And Chemicals, Inc. Dye penetrant method for detecting flows
US4204978A (en) 1977-09-06 1980-05-27 Den-Mat, Inc. Tooth crack detector
US4400618A (en) 1981-08-06 1983-08-23 International Business Machines Corporation Method of detecting and analyzing damage in printed circuit boards
US4858768A (en) * 1986-08-04 1989-08-22 The Coca-Cola Company Method for discrimination between contaminated and uncontaminated containers
WO1990000268A1 (en) 1988-07-01 1990-01-11 National Research Development Corporation Underwater inspection apparatus and method
US5554318A (en) 1994-02-07 1996-09-10 Basf Aktiengesellschaft Use of compounds which absorbs and fluoresce in the IR range as crack-detecting agents
WO2000047982A1 (en) 1999-02-08 2000-08-17 Tiede Gmbh & Co Rissprüfanlagen Crack detecting arrangement which is especially used after the dye-penetration method or magnetic method
WO2000060344A1 (en) 1999-03-31 2000-10-12 Hitachi, Ltd. Method and apparatus for non destructive testing
WO2005027730A2 (en) 2003-09-19 2005-03-31 The General Hospital Corporation Fluorescence polarization imaging devices and methods
US20060192177A1 (en) 2005-02-25 2006-08-31 Leishan Chen Essentially thickness independent single layer photoelastic coating
JP3141389U (en) 2008-02-19 2008-05-01 名古屋電機工業株式会社 Reference sample for fluorescence inspection and sample container used therefor
WO2013110458A1 (en) 2012-01-27 2013-08-01 Sartorius Stedim Biotech Gmbh Method for demonstrating flaws in an ultrafiltration membrane
WO2013144215A1 (en) 2012-03-29 2013-10-03 Roche Diagnostics Gmbh Method for training personnel in quality control
JP2015094642A (en) 2013-11-11 2015-05-18 マークテック株式会社 Flaw detector
US10054552B1 (en) 2017-09-27 2018-08-21 United Technologies Corporation System and method for automated fluorescent penetrant inspection
CN110862747A (en) 2019-11-28 2020-03-06 国网吉林省电力有限公司电力科学研究院 Penetrating agent for detecting and repairing encapsulated insulation microcracks and preparation method thereof

Patent Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3417241A (en) * 1965-12-07 1968-12-17 Ronald E. Davis Empty bottle inspection method and apparatus utilizing both water soluble and oil soluble fluorescent dyes
US3965350A (en) 1972-01-24 1976-06-22 Air Products And Chemicals, Inc. Dye penetrant method for detecting flows
US4204978A (en) 1977-09-06 1980-05-27 Den-Mat, Inc. Tooth crack detector
US4400618A (en) 1981-08-06 1983-08-23 International Business Machines Corporation Method of detecting and analyzing damage in printed circuit boards
US4858768A (en) * 1986-08-04 1989-08-22 The Coca-Cola Company Method for discrimination between contaminated and uncontaminated containers
WO1990000268A1 (en) 1988-07-01 1990-01-11 National Research Development Corporation Underwater inspection apparatus and method
US5554318A (en) 1994-02-07 1996-09-10 Basf Aktiengesellschaft Use of compounds which absorbs and fluoresce in the IR range as crack-detecting agents
WO2000047982A1 (en) 1999-02-08 2000-08-17 Tiede Gmbh & Co Rissprüfanlagen Crack detecting arrangement which is especially used after the dye-penetration method or magnetic method
WO2000060344A1 (en) 1999-03-31 2000-10-12 Hitachi, Ltd. Method and apparatus for non destructive testing
WO2005027730A2 (en) 2003-09-19 2005-03-31 The General Hospital Corporation Fluorescence polarization imaging devices and methods
US20060192177A1 (en) 2005-02-25 2006-08-31 Leishan Chen Essentially thickness independent single layer photoelastic coating
JP3141389U (en) 2008-02-19 2008-05-01 名古屋電機工業株式会社 Reference sample for fluorescence inspection and sample container used therefor
WO2013110458A1 (en) 2012-01-27 2013-08-01 Sartorius Stedim Biotech Gmbh Method for demonstrating flaws in an ultrafiltration membrane
WO2013144215A1 (en) 2012-03-29 2013-10-03 Roche Diagnostics Gmbh Method for training personnel in quality control
JP2015094642A (en) 2013-11-11 2015-05-18 マークテック株式会社 Flaw detector
US10054552B1 (en) 2017-09-27 2018-08-21 United Technologies Corporation System and method for automated fluorescent penetrant inspection
CN110862747A (en) 2019-11-28 2020-03-06 国网吉林省电力有限公司电力科学研究院 Penetrating agent for detecting and repairing encapsulated insulation microcracks and preparation method thereof

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