WO2022111710A1

WO2022111710A1 - Optical path conversion device and optical system

Info

Publication number: WO2022111710A1
Application number: PCT/CN2021/134157
Authority: WO
Inventors: 徐家宏; 闫红力; 吴江湖; 屈嘉乐; 林冲; 刘剑
Original assignee: 深圳市瑞图生物技术有限公司
Priority date: 2020-11-30
Filing date: 2021-11-29
Publication date: 2022-06-02
Also published as: CN114578535A; CN114578535B

Abstract

An optical path conversion device and an optical system. The optical path conversion device comprises: an objective lens assembly (300) and a conversion assembly (200). The objective lens assembly (300) comprises a first objective lens (321) and a second objective lens (322) to respectively form first emergent light (511) and second emergent light (520). The conversion assembly (200) comprises a first conversion mechanism (210) and a second conversion mechanism (220). The first conversion mechanism (210) receives the first emergent light (511) to form first reflected light (512). The second conversion mechanism (220) comprises a driving unit (228) and a reflecting unit (225). The driving unit (228) is connected to the reflecting unit (225) and drives the reflecting unit (225) to move to the optical path of the first reflected light (512), so as to form second reflected light (513) and make the optical path thereof coincide with the optical path of the second emergent light (520). The problem of poor precision caused by conventional structure conversion is solved by optical path conversion, and the reliability of the structure design of the optical path conversion is higher than that of the existing turntable structure, facilitating high-precision multiplicative conversion, facilitating control of the consistency of the optical paths, solving the difference in non parallelism between the objective lenses, and facilitating control of the degree to which the fields of view of the objective lenses coincide.

Description

Optical path conversion device and optical system

technical field

The present application relates to optical microscopy technology, in particular to an optical path conversion device and an optical system.

Background technique

Optical Microscope (OM) is an optical instrument that uses optical principles to magnify and image tiny objects that cannot be distinguished by the human eye, so that people can extract microstructure information. Taking a biological microscope as an example, for chromosome karyotype analysis, to observe the intermediate division of cells, it is necessary to use a low-power objective to scan the glass slide fully, and then use a high-power objective to observe the selected karyotype in detail. The low-power objective has a field of view. Compared with high magnification, the selected qualified cells are scattered in metaphase. Therefore, when switching from low magnification to high magnification objective lens, the positioning accuracy of the central field of view is very important. Poor positioning quality can easily lead to the phenomenon of lack of chromosome photography, which affects the judgment of results.

To observe any specimen, you must first determine the position of the target to be observed with a low-power objective lens. The product obtained by multiplying the magnification of the eyepiece and the magnification of the objective lens is the magnification of the original object; if the object image is not in the center of the field of view, slow down. Slowly move to the center of the field of vision, and then adjust appropriately. Then switch to a high-power objective lens. Under normal circumstances, when the high-power objective lens is turned positive, a blurred object image can be seen in the center of the field of view, and then fine-tune the focus to obtain a clear object image. When the high-magnification objective lens is replaced, the field of view becomes smaller and darker. To readjust the brightness of the field of view, it can be increased by raising the condenser or using a concave reflector.

Traditional commercial microscopes mostly use the objective lens conversion of the turntable structure. The conversion methods are divided into manual conversion and electric conversion. The number of objective lenses is divided into 3-hole, 4-hole and 6-hole turntables. There are fixed steel balls in the turntable, and each hole position corresponds to the design. The concave hole, through the positioning of the steel ball and the concave hole, determines the conversion positioning of the objective lens. However, it has the following problems: the conversion accuracy of the objective lens is poor. According to the high-power universal microscope in the national standard of "JBT 7398.7-1994", the two-way repeated positioning error of a single hole is ≤0.025mm; in fact, it is often close to 0.025mm. Moreover, the rotation of the rotating disk structure objective lens is positioned by the fixed steel ball and the concave hole, and the long-term back and forth conversion is easy to cause wear and tear, and the positioning accuracy is reduced; further, because the optical axis of the objective lens is not parallel to the mechanical axis, the non-parallelism of each objective There are differences, so after the objectives are mounted on the turntable, there are also differences in the positioning accuracy of the center field of view between the objectives.

SUMMARY OF THE INVENTION

Based on this, it is necessary to provide an optical path conversion device and an optical system.

An optical path conversion device, comprising: an objective lens assembly, including a first objective lens and a second objective lens, the first objective lens and the second objective lens are used to receive incident light respectively, so as to form a first outgoing light and a second outgoing light respectively a light; and a conversion assembly, including a first conversion mechanism and a second conversion mechanism, the first conversion mechanism is used for receiving the first outgoing light to form a first reflected light, and the second conversion mechanism includes a driving unit and a reflection a unit, the driving unit is connected to the reflection unit and used to drive the reflection unit to move to the optical path of the first reflected light, so as to form a second reflected light and make the optical path of the second reflected light and the light path of the second reflected light The optical paths of the second outgoing rays are coincident.

The above-mentioned optical path conversion device solves the problem of poor accuracy caused by traditional structural conversion through optical path conversion. On the one hand, the structural design of optical path conversion is more reliable than the current turntable structure, and on the premise of ensuring performance, it can meet the expected service life of the product; On the one hand, it is beneficial to achieve high-precision multiple conversion, and the accuracy can be controlled within ±0.005mm; on the other hand, it is beneficial to control the consistency of the optical path, solve the difference of non-parallelism between objective lenses, and control the coincidence of the fields of view of the objective lenses. On the other hand, it is not necessary to realize the beam integration of the objective lens through the prism combination, so as to avoid the problem of the optical path difference of the objective lens.

An optical system, comprising an illumination device, an imaging structure and any one of the optical path conversion devices; the illumination device is used for emitting the incident light to the first objective lens and the second objective lens; the imaging device The structure is arranged on the optical path of the second outgoing light.

Description of drawings

FIG. 1 is a schematic diagram of an embodiment of an optical path conversion device of the present application.

FIG. 2 is a schematic diagram of the position change of the second conversion mechanism of the embodiment shown in FIG. 1 .

FIG. 3 is a schematic diagram of another embodiment of the optical path conversion device of the present application.

FIG. 4 is a schematic diagram of another direction of the embodiment shown in FIG. 3 .

FIG. 5 is a schematic diagram of another direction of the embodiment shown in FIG. 3 .

FIG. 6 is a schematic diagram of an embodiment of the optical system of the present application.

FIG. 7 is a schematic diagram of the lighting device of the embodiment shown in FIG. 6 .

FIG. 8 is a schematic view from another direction of the embodiment shown in FIG. 7 .

FIG. 9 is a schematic diagram of another direction of the embodiment shown in FIG. 6 .

FIG. 10 is a schematic diagram of another direction of the embodiment shown in FIG. 6 .

FIG. 11 is a schematic diagram of another direction of the embodiment shown in FIG. 6 .

FIG. 12 is a schematic diagram of another direction of the embodiment shown in FIG. 6 .

FIG. 13 is an application schematic diagram of the embodiment shown in FIG. 6 .

FIG. 14 is a schematic view from another direction of the embodiment shown in FIG. 13 .

FIG. 15 is a schematic view from another direction of the embodiment shown in FIG. 13 .

FIG. 16A and FIG. 16B are respectively schematic diagrams of objective lens conversion of an embodiment of the optical system of the present application.

FIG. 17A and FIG. 17B are respectively schematic diagrams of objective lens conversion of another embodiment of the optical system of the present application.

Reference numerals: camera structure 100 , conversion assembly 200 , objective lens assembly 300 , lighting device 400 , frame structure 600 , support platform 700 , object loading device 800 ; first conversion mechanism 210 , second conversion mechanism 220 , reflection space 230 , objective lens The base 310, the objective lens group 320, the light source assembly 410, the light source guide rail 420, the light source driver 430, the base 440; the data connection port 101, the camera 102, the camera screw connection part 103, the magnification reducing lens 104, the connecting piece 105, the mounting part 106, Lens structure 107; mounting seat 211, first adjusting screw 212, second adjusting screw 213, reflector 214; machine table 221, fixing frame 222, frame 223, driving motor 224, reflecting unit 225, screw 226, third Conversion mechanism 227, drive unit 228; first objective lens 321, second objective lens 322, third objective lens 323; mounting card holder 411, mounting card slot 412, condensing lens group 413, condensing lens group 414, light-emitting lamp head 415, light source driving rod 431, light source base 432; incident light 500, first outgoing light 511, first reflected light 512, second reflected light 513, second outgoing light 520, third outgoing light 531, third reflected light 532, fourth reflected light 533.

Detailed ways

In order to make the above objects, features and advantages of the present application more clearly understood, the specific embodiments of the present application will be described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.

In addition, the terms "first" and "second" are only used for descriptive purposes, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature delimited with "first", "second" may expressly or implicitly include at least one of that feature. In the description of the present application, "plurality" means at least two, such as two, three, etc., unless expressly and specifically defined otherwise.

In this application, unless expressly stated and defined otherwise, a first feature "on" or "under" a second feature may be the first feature in direct contact with the second feature, or the first feature and the second feature indirectly contact through an intermediary. Also, the first feature being "above", "over" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is level higher than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature has a lower level than the second feature.

It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or an intervening element may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for the purpose of illustration only and do not represent the only embodiment.

In order to solve the problem of optical path difference, the heat dissipation of the light source and the structural design caused by the attenuation of the illumination brightness, the problem of the space size occupied by the lighting device, and the uncontrollable degree of the objective field of view because the sample introduction structure platform cannot be accurately moved in parallel. Problem, in one embodiment of the present application, an optical path conversion device includes: an objective lens assembly and a conversion assembly, the objective lens assembly includes a first objective lens and a second objective lens, and the first objective lens and the second objective lens are used to receive incident light respectively, The first outgoing light and the second outgoing light are respectively formed; in each embodiment, the incident light is received separately, and the incident light is received separately at different times, not simultaneously. The conversion assembly includes a first conversion mechanism and a second conversion mechanism. The first conversion mechanism is used to receive the first outgoing light to form the first reflected light. The second conversion mechanism includes a driving unit and a reflection unit. The driving unit is connected with the reflection unit and used for The reflection unit is driven to move on the optical path of the first reflected light, so as to form the second reflected light, and the optical path of the second reflected light is overlapped with the optical path of the second outgoing light. The above-mentioned optical path conversion device solves the problem of poor accuracy caused by traditional structural conversion through optical path conversion. On the one hand, the structural design of optical path conversion is more reliable than the current turntable structure, and on the premise of ensuring performance, it can meet the expected service life of the product; On the one hand, it is conducive to realizing high-precision multiple conversion, and the accuracy can be controlled within ±0.005mm; on the other hand, it is conducive to controlling the consistency of the optical path, that is, the observation direction, which solves the difference in the non-parallelism between the objective lenses, and is conducive to controlling the objective lens. On the other hand, it is not necessary to realize the beam integration of the objective lens through the combination of prisms, so as to avoid the problem of the optical path difference of the objective lens.

In one of the embodiments, an optical path conversion device includes part or all of the structures of the following embodiments; that is, the optical path conversion device includes some or all of the following technical features. In one embodiment, the optical path conversion device includes a conversion component and an objective lens component; the conversion component is used for controlling the optical path, and further, controlling the optical path includes keeping the optical path unchanged and adjusting the optical path; the objective lens component is used for providing an objective lens to form outgoing light, usually Objectives used to provide different magnifications. Further, in one of the embodiments, the objective lens assembly is used to maintain the position during the objective lens conversion, that is, the position remains unchanged. In the optical path conversion device of the present application, when the objective lens is converted, the objective lens itself is immobile, and the gap between the objective lens and the tube lens is fixed. The optical path is an almost ideal parallel light, so the parfocal distance of the objective lens is strictly controlled; because the mirror is changed and the beam combination is not realized by prism combination, the problem of optical path difference is avoided.

In order to facilitate the conversion of the objective lens, in one embodiment, the objective lens assembly includes a first objective lens and a second objective lens, and the first objective lens and the second objective lens are used to receive incident light respectively, so as to form the first outgoing light and the second outgoing light respectively; Further, in one of the embodiments, each objective lens in the objective lens assembly includes a first objective lens and a second objective lens, and the optical axes thereof are parallel to each other; that is, the first outgoing light rays and the second outgoing light rays are parallel. Further, in one of the embodiments, the objective lens assembly further includes a third objective lens, and the third objective lens is used for receiving incident light rays to form third outgoing light rays. Further, in one of the embodiments, the direction of the second outgoing light is coincident with the observation direction. Such a design is conducive to the realization of high-precision multiple conversion, the accuracy can be controlled within ±0.005mm, and it is beneficial to control the consistency of the optical path, solve the difference in the non-parallelism between the objective lenses, and thus help to control the coincidence of the fields of view of the objective lenses. Spend.

In order to keep the position of the objective lens unchanged during the conversion of the objective lens, in one embodiment, the conversion component includes a first conversion mechanism and a second conversion mechanism, and the first conversion mechanism is configured to receive the first outgoing light to form the first reflected light, The second conversion mechanism includes a driving unit and a reflecting unit. The driving unit is connected to the reflecting unit and is used to drive the reflecting unit to move to the optical path of the first reflected light, so as to form the second reflected light and make the optical path of the second reflected light and the second exit. The light paths of the rays coincide. Further, in one of the embodiments, the conversion component is provided with a reflection space between the first conversion mechanism and the second conversion mechanism, and the optical path of the first reflected light is located in the reflection space. In each embodiment, both the first conversion mechanism and the second conversion mechanism are provided with total reflection mirrors, that is, the reflector of the first conversion mechanism and the reflection unit of the second conversion mechanism are provided with total reflection mirrors; in one embodiment In one embodiment, the first conversion mechanism and the second conversion mechanism are provided with a total reflection prism; in one embodiment, the first conversion mechanism and the second conversion mechanism are provided with a right angle prism; such a design, on the one hand, adopts a total reflection prism It is beneficial to standardize the reflection direction and reduce the adjustment of the optical path alignment; with the repeated use of the product, the adjustment of the optical path alignment is sometimes necessary, so effective and efficient adjustment needs to be considered; on the other hand, the optical path conversion When the objective lens remains stationary, the problem of poor structural conversion accuracy is solved through optical path conversion. On the other hand, the structural design of optical path conversion is more reliable than the current rotating disk objective lens assembly, and on the premise of ensuring performance, it can meet the expected service life of the product.

In one embodiment, the number of driving motors is one, and in one embodiment, the movement direction of the reflection unit is perpendicular to the second reflected light and the second outgoing light. Further, in one of the embodiments, the movement direction of the reflection unit is perpendicular to the second reflected light and the second outgoing light, so that the reflection unit has a linear reflection range, and at any position within the linear reflection range, the second reflection The light paths of the light rays are all coincident with the light paths of the second outgoing light rays. This is an excellent technical solution. Since the movement direction of the reflection unit is perpendicular to the second reflection light and the second outgoing light, during the movement of the reflection unit, it only needs to enter the reflection position, that is, the light entering the first reflection light. On the road, since the reflection angle is constant, the reflection position, that is, the reflection direction, remains constant, so that the second reflected light can be formed whose optical path coincides with the optical path of the second outgoing light. The optical path conversion efficiency of the optical path conversion device is greatly improved, the technical problem of repeatedly adjusting the reflection direction is avoided, the workload of manual intervention is reduced, and it is conducive to rapid positioning and further automatic detection.

In order to achieve the effect of converting the objective lens when the objective lens does not move, in one embodiment, the second converting mechanism further includes a fixing frame, the driving unit includes a driving motor and a lead screw, the driving motor is installed on the fixing frame, and the reflection The unit is installed on the screw rod, the driving motor drives the screw rod to move and drives the reflection unit to move along the screw rod, and the extension direction of the screw rod is perpendicular to the second reflected light and the second outgoing light. That is, the drive motor is used to adjust the relative position of the optical path of the reflection unit and the first reflected light according to the selection requirements of the objective lens, to control the reflection optical path of the reflection unit, in one embodiment, the drive motor is used to select the requirements according to the objective lens along a The direction adjusts the relative position of the reflection unit and the first reflected light. In one of the embodiments, the driving motor is a stepping motor or a linear motor. Such a design is conducive to accurately controlling the position of the reflection unit. The structural design of the optical path conversion is more reliable than the current turntable structure, and can meet the expected service life of the product on the premise of ensuring performance.

In order to facilitate the control of the reflection angle, so that the optical path enters the observation direction after being accurately reflected, in one embodiment, the first conversion mechanism includes a mounting seat, a reflecting member and an adjusting member, and the adjusting member is respectively connected with the mounting base and the reflecting member, and adjusts the The part is used to adjust the inclination angle of the reflection part relative to the first outgoing light. In order to adjust the reflection angle of the reflecting member to accurately control the reflected light path, further, in one embodiment, the adjusting member includes two adjusting screws, which are arranged at intervals and are respectively installed on the mounting bases for adjusting The reflection angle of the reflector. Alternatively, the adjustment member includes three adjustment screws that are not in the same line. Such a design, on the one hand, is conducive to fine-tuning the optical path so that it can accurately enter the observation direction after being reflected; on the other hand, by adjusting the angle of the mirror, the low-magnification objective lens matches the high-power objective lens, which is conducive to solving the difference in non-parallelism between the objective lenses .

In one embodiment, the objective lens assembly further includes at least one third objective lens, the third objective lens is used for receiving the incident light to form the third outgoing light, and the first conversion mechanism is further used for moving to the optical path of the third outgoing light to form the third outgoing light. For the third reflected light, the driving unit is further configured to drive the reflecting unit to move on the optical path of the third reflected light to form a fourth reflected light, and the optical path of the fourth reflected light is overlapped with the optical path of the second outgoing light. The number of third objective lenses may be one, two or more, that is, there may be multiple third objective lenses, but only one first conversion mechanism, and this first conversion mechanism can be moved so that the third objective lens originating from the third objective lens The three outgoing rays are reflected by the first converting mechanism to form a third reflected light whose direction is controlled, and then are correspondingly reflected by the reflecting unit of the second converting mechanism to form a fourth reflected light that overlaps the optical path of the second outgoing light. The imaging effects of different objective lenses are obtained at the same observation position, and the purpose of objective lens conversion is achieved under the premise that the objective lens does not move.

In one embodiment, the objective lens assembly further includes a third objective lens, and the third objective lens is used for receiving the incident light to form the third outgoing light, and the conversion assembly further includes a third conversion mechanism, and the third conversion mechanism is used for receiving the third outgoing light. In order to form the third reflected light, the driving unit is also used for driving the reflecting unit to move to the optical path of the third reflected light to form the fourth reflected light, and the optical path of the fourth reflected light and the optical path of the second outgoing light are overlapped. When the second objective lens is used for imaging, the second objective lens does not move, and the incident light irradiates the sample to be detected and passes through the second objective lens to form a second outgoing light. The sample to be tested, the incident light irradiates the sample to be tested and then passes through the first objective lens to form the first outgoing light, the first conversion mechanism receives the first outgoing light to form the first reflected light, and the driving unit drives the reflecting unit to move to the light of the first reflected light on the way, so as to form the second reflected light and make the light path of the second reflected light coincide with the light path of the second outgoing light. In this case, when the third objective lens is used for imaging, the first conversion mechanism can be immobile; if necessary, in order to avoid blocking the optical path of the third reflected light, the first conversion mechanism can also be movable. In an embodiment, the optical path conversion device further includes a conversion drive assembly, which is connected to the first conversion mechanism and used to drive the first conversion mechanism to move so that the first conversion mechanism is away from the optical path of the third reflected light. Further, in one of the embodiments, the conversion drive assembly includes a motor and a connecting rod, and the motor is connected to the first conversion mechanism through the connecting rod; or, the conversion drive assembly includes a frame device and a stepping motor fixed on the frame device, and the step The feeding motor is connected with the first conversion mechanism through a connecting rod, and is used for driving the first conversion mechanism away from the optical path of the third reflected light or close to the optical path of the third reflected light at a predetermined distance each time. For the embodiment in which the first conversion mechanism does not move, in order to use the light path to reduce adjustment as much as possible, further, in one of the embodiments, the number of objective lenses in the objective lens group is one more than the number of the first conversion mechanism, and the light of one objective lens is The axis is used to coincide with the observation direction, and the positions of the remaining objective lenses correspond to the first conversion mechanism one by one. With such a design, the objective lens whose optical axis coincides with the observation direction does not need to use the first conversion mechanism during use; other objective lenses correspond to a first conversion mechanism, and the corresponding first conversion mechanism directly adjusts the first conversion mechanism when in use. The light is reflected so that the direction of the second reflected light is coincident with the optical axis of the objective lens.

In order to be applicable to the objective lens assembly of more objective lenses, in one embodiment, the number of third objective lenses is at least two, the number of third conversion mechanisms is at least two, and each third objective lens and each third conversion mechanism One-to-one corresponding settings. For the embodiment in which there is a third objective lens and the first conversion mechanism is immovable, in order to correspond to a plurality of objective lenses, the position of the reflection unit can be controlled in two directions. In one embodiment, the number of driving motors is two, and the two driving motors are used to adjust the relative positions of the reflection unit and the optical path in two directions according to the selection requirements of the objective lens. Further, the driving directions of the two driving motors are perpendicular to each other. In one embodiment, the number of the first conversion mechanisms is at least two, and the reflectors in each of the first conversion mechanisms are spaced apart from each other so that they do not block each other; in the objective lens group, the number of objective lenses is larger than that of the first conversion mechanism. One more in number, the optical axis of one of the objective lenses is used to coincide with the observation direction, and the positions of the remaining objective lenses correspond to the reflectors of the first conversion mechanism one by one. Such a design is conducive to realizing accurate switching of multiple objective lenses.

In one embodiment, an optical path conversion device is shown in FIG. 1 and FIG. 2 , which includes a conversion assembly 200 and an objective lens assembly 300; the objective lens assembly 300 includes an objective lens holder 310 and an objective lens group 320 mounted on the objective lens holder 310, The objective lens group 320 includes a first objective lens 321 and a second objective lens 322. Please refer to FIG. 2 together. The first objective lens 321 and the second objective lens 322 are used to receive the incident light 500 respectively to form the first outgoing light 511 and the second outgoing light respectively. Light 520 ; wherein, the direction of the first outgoing light 511 and the direction of the second outgoing light 520 are respectively consistent with the direction of the incident light 500 . The conversion assembly 200 includes a first conversion mechanism 210 and a second conversion mechanism 220, and a reflection space 230 is disposed between the first conversion mechanism 210 and the second conversion mechanism 220; the second conversion mechanism 220 is used to form the second reflected light 513 , and make the optical path of the second reflected light 513 coincide with the optical path of the second outgoing light 520, the second conversion mechanism 220 adjusts the position according to the selection requirements of the objective lens to control whether the optical path needs to be changed; the first conversion mechanism 210 is used to receive the first outgoing light. The light 511 is used to form the first reflected light 512. If necessary, the reflection angle can be adjusted to control the fine direction of the first reflected light 512 to accurately correspond to the first outgoing light 511 and the second conversion mechanism 220; the second conversion mechanism 220 includes The driving unit 228 and the reflecting unit 225, the driving unit 228 is connected with the reflecting unit 225 and is used to drive the reflecting unit 225 to move to the optical path of the first reflected light 512 to form the second reflected light 513 and make the optical path of the second reflected light 513 and The optical paths of the second outgoing light rays 520 overlap. In this embodiment, the objective lens group 320 includes two objective lenses, and the optical axes of each objective lens can be arranged in parallel. In the embodiment shown in FIG. 1 , the second converting mechanism 220 cooperates with the first converting mechanism 210 to change the direction of the first outgoing light 511 . As shown in FIG. 2, the position of the second conversion mechanism 220 is adjusted to keep the direction of the second outgoing light 520 unchanged, so that the optical path of the second reflected light 513 shown in FIG. The optical paths of the outgoing light rays 520 are overlapped for easy observation or detection. In this embodiment, both the first conversion mechanism 210 and the second conversion mechanism 220 are provided with right-angle prisms.

Further, in one of the embodiments, the optical path conversion device further includes a camera structure and a frame structure, the camera structure is fixed on the frame structure, the conversion component is located between the camera structure and the objective lens component, and the camera structure is located in the light of the second outgoing light. On the way, it is used to obtain an enlarged image of the sample to be tested; in one embodiment, the imaging structure is provided with an adaptor mirror. Further, in one of the embodiments, the first conversion mechanism is fixed on the frame structure. It can be understood that the imaging structure can be replaced with an eyepiece structure for easy observation. Further, in one of the embodiments, the optical path conversion device further includes a control device connected to the imaging structure, and the control device is configured to monitor the state of the observation field through the imaging structure and control the imaging structure to perform automatic imaging. Further, in one of the embodiments, the imaging structure includes a camera and its data connection port, a magnification reducing lens, a connector, a mounting portion and a lens structure, and the camera is connected to the magnification reducing lens through the camera screw connection portion therein, and the magnification reducing lens is reduced. The reducing mirror is connected to the lens structure through a connecting piece, the lens structure includes a lens and its mounting seat, a mounting portion is disposed outside the connecting piece, and the camera structure is fixed to the outside through the mounting portion, for example, to the frame structure.

In one embodiment, an optical path conversion device is shown in FIG. 3 . Different from the embodiment shown in FIG. 1 , the optical path conversion device of this embodiment further includes a camera structure 100 and a frame structure 600 , and the camera structure 100 is fixed On the frame structure 600 , the conversion component 200 is located between the imaging structure 100 and the objective lens component 300 , and the imaging structure 100 is located on the optical path of the second outgoing light.

Please refer to FIG. 4 and FIG. 5 together, the first converting mechanism 210 is provided with a mounting seat 211 , a first adjusting screw 212 , a second adjusting screw 213 and a reflector 214 ; the reflector 214 is adjusted by the first adjusting screw 212 and the second adjusting screw 214 The screw 213 is disposed on the mounting seat 211 , and the reflection angle of the reflector 214 is adjusted by the first adjusting screw 212 and the second adjusting screw 213 respectively. The second conversion mechanism 220 is provided with a machine table 221 , a fixing frame 222 , a driving motor 224 , a reflection unit 225 and a lead screw 226 ; the driving motor 224 is arranged on the fixing frame 222 , the fixing frame 222 is arranged on the machine table 221 , and the driving motor 224 Driven and connected to the reflection unit 225 through the screw rod 226, the driving motor 224 is used to adjust the relative position of the reflection unit 225 and the optical path according to the selection requirements of the objective lens, so as to control the optical path of the second outgoing light. In one embodiment, the drive motor 224 is used to adjust the relative position of the reflection unit 225 and the optical path in one direction according to the selection requirements of the objective lens. The objective lens group 320 is provided with a first objective lens 321 and a second objective lens 322 mounted on the objective lens holder 310 , and the optical axes of the first objective lens 321 and the second objective lens 322 are arranged in parallel.

In one embodiment, an optical system includes an optical path conversion device of any of the embodiments. In one of the embodiments, an optical system includes an illumination device, an imaging structure and any one of the optical path conversion devices; the illumination device is used for emitting incident light to the first objective lens and the second objective lens; the imaging structure is arranged on the second objective lens. on the light path of the outgoing light. In one embodiment, the lighting device includes a light source and a moving component, the moving component drives the light source to move, and the light source is used to form incident light. In one embodiment, the condensing and outgoing direction of the illumination device is arranged parallel to the optical axis of each objective lens, and the illumination device can be arranged in translation toward the objective lens group so that the condensing and outgoing direction of the illumination device coincides with the optical axis of the selected objective lens. . It can be understood that the optical axis of the objective lens is usually a straight line, and the condensing and exiting direction of the illuminating device is usually a beam range. . Further, in one of the embodiments, the light source is a light source assembly, and the moving assembly includes a light source guide rail, a light source driver and a base; that is, the lighting device includes a light source assembly, a light source guide rail, a light source driver and a base, and the light source driver and the light source guide rail are arranged on the base. , the light source assembly is arranged on the light source guide rail, the light source driver is drivingly connected with the light source assembly, and the light source driver is used to drive the light source assembly to slide on the light source guide rail, so that the condensing and outgoing direction of the light source assembly coincides with the optical axis of the selected objective lens.

In one of the embodiments, an optical path conversion device is shown in FIG. 6 . Different from the embodiment shown in FIG. 1 , the optical path conversion device of this embodiment further includes a camera structure 100 and an illumination device 400 . and lighting device, so the optical path conversion device of this embodiment can also be called an optical system, that is, an optical system with an optical path conversion device, that is, an optical system as shown in FIG. , the optical system of this embodiment does not include the frame structure 600, but has an additional lighting device 400. The second conversion mechanism 220 is further provided with a frame 223, the reflection unit 225 is arranged on the frame 223, and the driving motor 224 passes through the screw 226. It is drivingly connected with the frame 223 and drives the reflection unit 225 . In this embodiment, the imaging structure 100 includes a camera 102 and its data connection port 101 , a magnification reduction lens 104 , a connector 105 , a mounting portion 106 and a lens structure 107 , and the camera 102 is connected to the reduction magnification through the camera screw connection portion 103 therein. The mirror 104, the magnification and reduction mirror 104 are connected to the lens structure 107 through the connecting member 105. The lens structure 107 includes a lens and its fixing seat. The connecting member 105 is provided with a mounting portion 106. on the frame structure 600. The lighting device 400 includes a light source assembly 410, a light source guide rail 420, a light source driver 430 and a base 440. The light source driver 430 and the light source guide rail 420 are arranged on the base 440, the light source assembly 410 is slidably arranged on the light source guide rail 420, and the light source assembly 410 is used to provide incident light. For the light 500, the light source driver 430 is drivingly connected with the light source assembly 410, and the light source driver 430 is used to drive the light source assembly 410 to slide on the light source guide rail 420, so that the condensing and outgoing direction of the light source assembly 410 coincides with the optical axis of the selected objective lens.

In one embodiment, the lighting device is shown in FIG. 7 and FIG. 8 . The lighting device is further sleeved with a mounting bracket 411 on the light source assembly 410 , and the mounting bracket 411 is provided with a mounting slot 412 for installing and fixing the light source. The condensing lens group at the end of the assembly 410; the lighting device is also provided with a light source seat 432, the light source assembly 410 is fixed on the light source seat 432 and is slidably connected with the light source guide rail 420, and the light source driving rod 431 of the light source driver 430 is connected to the light source seat 432, and passes through the light source. The driving rod 431 drives the light source base 432 to drive the light source assembly 410 to slide on the light source guide rail 420 .

In one embodiment, an optical system is shown in FIG. 9 . Different from the embodiment shown in FIG. 6 , the optical system of this embodiment further includes a support platform 700 . Please refer to FIGS. 10 , 11 and 700 together. FIG. 12 , the frame structure 600 is fixed on the support platform 700 .

In one embodiment, the optical system further includes a loading device, the loading device includes a loading stage and a power unit, the loading stage is arranged on the optical path of the incident light and is used for carrying the sample to be detected, and the power unit is connected with the loading stage , the power unit is used to drive the movement of the stage to move the sample to be detected into the observation field of the first objective lens and the second objective lens.

In one embodiment, an optical system is shown in FIG. 13 . Different from the embodiment shown in FIG. 9 , the optical system of this embodiment further includes an object carrier device 800 , please refer to FIGS. 14 and 15 together. , the loading device 800 is used as a sample platform, and a plurality of samples to be tested are sequentially arranged on the loading device 800. In actual use, automatic control of sample injection, automatic control of switching objective lens, automatic camera, and finally can also cooperate with the judgment module to achieve automatic Judgment, so as to complete the automatic detection of samples.

In one embodiment, the objective lens switching of an optical system is shown in FIG. 16A and FIG. 16B . The lighting device further includes a light-emitting lamp head 415 , a condensing lens group 413 and a condensing lens group 414 , and the light emitted by the light-emitting lamp head 415 is generally dispersed. , the incident light 500 is formed after being collected by the condensing lens group 413 and the condensing lens group 414 , as shown in FIG. 16A , enters the second objective lens 322 , passes through the lens structure 107 and forms a second outgoing light 520 , and is then imaged on the camera 102 ; The optical axis of the second objective lens 322 coincides with the condensing and outgoing direction of the condensing lens group 414, and neither the reflector 214 of the first conversion mechanism 210 nor the reflection unit 225 of the second conversion mechanism 220 changes the direction of the second outgoing light 520; In the embodiment, in the objective lens group 320, the number of objective lenses is one more than the number of the first conversion mechanism 210, and the optical axis of the second objective lens 322 is used to coincide with the observation direction, and the position of the first objective lens 321 corresponds to the first conversion mechanism. Institution 210. When changing the objective lens, as shown in FIG. 16B , the objective lens does not move, and the camera 102 does not move either, and the position of the light source assembly of the lighting device is adjusted to adjust the position of the light-emitting lamp head 415 . At this time, the optical axis of the first objective lens 321 and the condenser lens group 414 The first objective lens 321 receives the incident light 500 to form the first outgoing light 511, the reflector 214 reflects the first outgoing light 511 to form the first reflected light 512, and the reflecting unit 225 reflects The first reflected light 512 forms the second reflected light 513 and the optical path of the second reflected light 513 is overlapped with the optical path of the second outgoing light 520, while the positions of the camera 102 and the lens structure 107 remain unchanged, that is, the observation position remains unchanged, That is to say, the position of the light-emitting imaging remains unchanged, and the positions of the first objective lens 321 and the second objective lens 322 also remain unchanged. Only the position of the reflection unit 225 of the second conversion mechanism 220 has changed to control the second reflected light. The optical path of 513 coincides with the optical path of the second outgoing light 520, so that the effect of objective lens switching is achieved on the premise that the positions of the above items remain unchanged. In addition, the angle of the reflector 214 of the first conversion mechanism can be fine-tuned to ensure the accuracy of the light path entering the lens structure 107 and the camera 102 .

In one of the embodiments, the objective lens switching of an optical system is shown in FIGS. 17A and 17B . Different from the embodiment shown in FIGS. 16A and 16B , the objective lens group of the optical system in this embodiment further includes a third The objective lens 323, that is, the objective lens assembly includes three objective lenses, namely the first objective lens 321, the second objective lens 322 and the third objective lens 323. Similarly, the third objective lens 323 is used to receive the incident light 500 to form the third outgoing light 531, and convert the The assembly also includes a third conversion mechanism 227, wherein the optical axis of the second objective lens 322 is used to coincide with the observation direction, and the positions of the first objective lens 321 and the third objective lens 323 correspond to the reflector 214 and the third conversion mechanism 227 respectively; The conversion mechanism 227 is used to receive the third outgoing light 531 to form the third reflected light 532, and the driving unit 228 is also used to drive the reflecting unit 225 to move to the optical path of the third reflected light 532 to form the fourth reflected light 533, and make the fourth reflected light 533. The optical path of the fourth reflected ray 533 coincides with the optical path of the second outgoing ray 520. Similarly, the positions of the first objective lens 321, the second objective lens 322, the third objective lens 323, the camera 102 and the lens structure 107 remain unchanged, only The positions of the incident light 500 and the reflection unit 225 are changed. In this embodiment, the number of driving motors is two, and the two driving motors are used to adjust the relative positions of the reflection unit 225 and the optical path in two directions according to the selection requirements of the objective lens, so as to correspond to the reflection member 214 and the third conversion mechanism 227 respectively. . The first conversion mechanism 210 and the third conversion mechanism 227 are spaced apart from each other so as not to block each other. In another embodiment, the first conversion mechanism 210 further includes a conversion driving component, and the conversion driving component may be a screw motor or a linear stepping motor, etc., and the first conversion mechanism 210 and the third conversion mechanism 227 may be located on the same plane, When the third outgoing light ray 531 needs to be reflected to form the third reflected light ray 532, the conversion driving assembly moves so that the first conversion mechanism 210 is far away from the optical path of the third reflected light ray 532. Therefore, even if the first conversion mechanism 210 and the third conversion mechanism The mechanism 227 is located on the same plane, and the conversion drive assembly can also prevent the first conversion mechanism 210 from blocking the third reflected light 532 . Further, in one of the embodiments, the optical axes of each objective lens in the objective lens group are arranged in parallel and located on the same plane.

As shown in FIG. 17A , the direction range of the light emitted by the light-emitting lamp head 415 of the lighting device is generally relatively large and scattered. The incident light 500 is formed after being collected by the condensing lens group 413 and the condensing lens group 414 , and enters the first objective lens 321 to form the first objective lens 321 When the light 511 is emitted, the optical axis of the first objective lens 321 is coincident with the light-converging and output direction of the condensing lens group 414, the first emitted light 511 is reflected by the reflecting member 214 of the first conversion mechanism to form the first reflected light 512, and then After being reflected by the reflection unit 225 of the second conversion mechanism, the second reflected light 513 is formed and enters the lens structure 107 and the camera 102, and is finally imaged on the camera 102; when the objective lens is converted, as shown in FIG. 17B, adjust the position of the light source component of the lighting device That is to say, the position of the light-emitting lamp head 415 is adjusted. At this time, the optical axis of the third objective lens 323 coincides with the condensing and outputting direction of the condenser lens group 414, so that the incident light 500 enters the third objective lens 323 to form a third outgoing light 531. The third outgoing light 531 is reflected by the third conversion mechanism 227 to form a third reflected light 532, and then reflected by the reflecting unit 225 to form a fourth reflected light 533, which is finally imaged on the camera 102; during this process, the positions of the camera 102 and the lens structure 107 remain unchanged , that is, the observation position remains unchanged, and the positions of the first objective lens 321, the second objective lens 322 and the third objective lens 323 also remain unchanged, only the positions of the light-emitting lamp head 415 and the reflecting unit 225 have changed to control the second output The optical path of the light 520 achieves the effect of objective lens switching on the premise that the positions of the above items remain unchanged. The reflection angle of the third conversion mechanism 227 can be fine-tuned to ensure the accuracy of the light path entering the lens structure 107 and the camera 102 .

Please refer to FIG. 6 , FIG. 13 , FIG. 16A , FIG. 16B , FIG. 17A and FIG. 17B , in one embodiment, the entire microscope imaging optical system includes a camera structure 100 , a conversion component 200 , an objective lens component 300 , and an illumination device 400 And the object carrier device 800; wherein the lighting device includes an LED light source with a light-emitting lamp head 415, a condensing lens group 413 and a condensing lens group 414, and the condensing lens group 413 includes 2 lenses such as a plano-convex lens and a biconvex lens. The condensing lens group is shown in the figure 413 is the implementation case without excluding other optical structures, the same below; the condenser lens group 414 includes two lenses such as a biconvex lens and a hyperspherical plano-convex lens, and the object carrier includes a glass slide, a sample and a cover glass, etc. The object carrier can be fixed on XY stage such as manual XY stage or motorized XY stage; or the object loading device can include XY stage; the objective lens assembly includes objective lens, tube lens and adapter lens, etc. The matching of glasses does not exclude the combination of objective lenses and adapter lenses with other multiples; the conversion assembly includes a first conversion mechanism and a second conversion mechanism.

In this embodiment, please continue to refer to FIG. 16B , when the first objective lens 321 such as the 10x objective lens is used for observation, the light from the light-emitting lamp head 415 of the LED lamp is collected by the light-collecting lens group 413 , because the light-emitting angle of the general LED lamp is relatively large. , the light needs to be collected, and then condensed to the surface of the sample through the condenser lens group 414 to illuminate the sample. After the 10x objective lens imaging light passes through the optical path of the objective lens, the light is reflected by the reflector 214 of the first conversion mechanism 210 at 90 degrees to the reflection unit 225 of the second conversion mechanism 220. After the second 90-degree reflection, it enters the tube lens, 0.5x adapter mirror and camera in turn. The function of the adapter mirror is to reduce the size of the imaging field of view to match the size of the target surface of the camera chip. The LED lights of the lighting device 400 can be in at least two situations: (1) There is a corresponding LED light under the objective lens of each multiple. When the low-power objective lens is working, the first LED light is controlled to be turned on; when switching to the high-power lens, the first LED light is controlled. One LED light is turned off, and the second LED light is controlled to be turned on, and the rest of the embodiments are analogous, and will not be repeated; ② One LED light, which moves with the lighting device, adjusts the brightness of the LED light to fit the objective lens. From the standpoint of heat dissipation and energy saving, the latter is preferred.

Please continue to refer to FIG. 16A , when switching to the second objective lens 322, such as a 100x objective lens for observation, the reflection unit 225 moves out of the optical path system, and at the same time the illuminating device moves below the optical axis of the 100x objective lens, and the sample is moved to the 100x observation field through the XY stage. The objective lens is switched when the objective lens position remains unchanged. The reflector 214 of the first conversion mechanism 210 adjusts the reflection angle to control the optical path of the second outgoing light, so that the optical axis of the 10x objective lens can be parallel to the optical axis of the 100x objective lens, and can match the optical axis parallelism of different objective lenses.

That is, the present application realizes the selection of the objective lens multiple by means of the mirror assembly, including the first conversion mechanism and the second conversion mechanism, switching in the optical path system, wherein the switching of the mirror such as the prism can be realized by a linear motion mechanism, Moreover, there is no requirement for the accuracy of the linear motion, only the prism reflection area can satisfy the effective reflection area, and the utility model has the advantages of simple implementation, high precision and long service life. For example, for the linear motion mechanism of moving prism, the screw rod with stepper motor or linear motor can be selected. These motion modes can withstand stronger work intensity. In the application scenario of low work intensity of prism switching, it is calculated according to the maximum daily test volume. The service life can reach more than 5 years, far exceeding the traditional expected validity period, so the influence of the wear of the moving mechanism on the accuracy can be reduced to a very low level.

It should be noted that other embodiments of the present application also include implementable optical path conversion devices and optical systems formed by combining the technical features of the above embodiments.

The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity, all possible combinations of the technical features in the above-described embodiments are not described. However, as long as there is no contradiction between the combinations of these technical features, All should be regarded as the scope described in this specification. Moreover, the above-mentioned embodiments only represent several embodiments of the present application, and the descriptions thereof are relatively specific and detailed, but should not be construed as a limitation on the scope of the patent application. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the protection scope of the present application. Therefore, the scope of protection of the patent of the present application shall be subject to the appended claims.

Claims

An optical path conversion device, characterized in that it comprises:

an objective lens assembly, comprising a first objective lens and a second objective lens, the first objective lens and the second objective lens are used for respectively receiving incident light rays to form first outgoing light rays and second outgoing light rays respectively; and

The conversion assembly includes a first conversion mechanism and a second conversion mechanism, the first conversion mechanism is used for receiving the first outgoing light to form a first reflected light, the second conversion mechanism includes a driving unit and a reflection unit, so The driving unit is connected with the reflection unit and is used to drive the reflection unit to move to the optical path of the first reflected light, so as to form a second reflected light and make the optical path of the second reflected light and the second outgoing light The light paths of the rays coincide.
The optical path conversion device according to claim 1, wherein the objective lens assembly is used to keep the position unchanged during the objective lens conversion.
The optical path conversion device according to claim 1, wherein the optical axis of the first objective lens and the optical axis of the second objective lens are parallel to each other.
The optical path conversion device according to claim 1, wherein the direction of the second outgoing light is coincident with the observation direction.
The optical path conversion device according to claim 1, wherein the conversion component is provided with a reflection space between the first conversion mechanism and the second conversion mechanism, and the optical path of the first reflected light is located in the in the reflection space.
The optical path conversion device according to claim 1, wherein a total reflection mirror is provided in the first conversion mechanism and the second conversion mechanism.
The optical path conversion device according to claim 1, wherein the first conversion mechanism and the second conversion mechanism are both provided with a total reflection prism.
The optical path conversion device according to claim 1, wherein the first conversion mechanism and the second conversion mechanism are both provided with right-angle prisms.
The optical path conversion device according to claim 1, wherein a movement direction of the reflection unit is perpendicular to the second reflected light and the second outgoing light.
The optical path conversion device according to claim 1, wherein the first conversion mechanism comprises a mounting seat, a reflecting member and an adjusting member, the adjusting member is respectively connected to the mounting base and the reflecting member, and the adjusting member The member is used to adjust the inclination angle of the reflection member relative to the first outgoing light.
The optical path conversion device according to claim 10, wherein the adjusting member comprises two adjusting screws, and the two adjusting screws are arranged at intervals and are respectively mounted on the mounting seat for adjusting the reflecting member or, the adjustment member includes three adjustment screws that are not on the same straight line.
The optical path conversion device according to claim 1, wherein the driving unit comprises a driving motor, and the driving motor is used to adjust the relative position of the reflection unit and the optical path of the first reflected light according to the selection requirement of the objective lens , to control the reflection light path of the reflection unit.
The optical path conversion device according to claim 12, wherein the driving unit further comprises a screw rod, the reflection unit is mounted on the screw rod, and the driving motor drives the screw rod to move to drive the reflection The unit moves along the screw rod, and the extension direction of the screw rod is perpendicular to the second reflected light and the second outgoing light.
The optical path conversion device according to claim 12, wherein the number of the driving motor is one, and the driving motor is used to adjust the distance between the reflection unit and the first reflected light in one direction according to the selection requirement of the objective lens. Or, the number of the driving motors is two, and the two driving motors are used to adjust the relative positions of the reflection unit and the first reflected light in two directions according to the selection requirements of the objective lens.
The optical path conversion device according to claim 14, wherein the number of the driving motors is two, and the driving directions of the two driving motors are perpendicular to each other.
The optical path conversion device according to claim 12, wherein the second conversion mechanism further comprises a fixing frame, and the driving motor is mounted on the fixing frame.
The optical path conversion device according to claim 1, wherein the objective lens assembly further comprises at least one third objective lens, the third objective lens is used for receiving incident light rays to form third outgoing light rays, and the first conversion mechanism It is also used to move to the optical path of the third outgoing light to form a third reflected light, and the driving unit is also used to drive the reflecting unit to move to the optical path of the third reflected light to form a fourth reflection light, the optical path of the fourth reflected light is coincident with the optical path of the second outgoing light.
The optical path conversion device according to claim 1, wherein the objective lens assembly further comprises a third objective lens, the third objective lens is used for receiving incident light rays to form third outgoing light rays, and the conversion assembly further comprises a third objective lens a conversion mechanism, the third conversion mechanism is used for receiving the third outgoing light to form a third reflected light, and the driving unit is further used for driving the reflecting unit to move to the optical path of the third reflected light, so as to A fourth reflected light ray is formed, and the optical path of the fourth reflected light ray is coincident with the optical path of the second outgoing light ray.
The optical path conversion device according to claim 17, wherein the number of the third objective lens can be one, two or more, and there is only one of the first conversion mechanism.
The optical path conversion device according to claim 18, wherein the first conversion mechanism is immovable, the drive unit includes a drive motor, the number of the drive motors is two, and the two drive motors are used for According to the selection requirements of the objective lens, the relative positions of the reflection unit and the optical path are adjusted in two directions to respectively correspond to the first conversion mechanism and the third conversion mechanism, wherein the first conversion mechanism and the third conversion mechanism spaced from each other so that they do not block each other.
The optical path conversion device according to claim 17, wherein the optical path conversion device further comprises a conversion driving assembly, the conversion driving assembly is connected with the first conversion mechanism, and is used for driving the first conversion mechanism to move so that the first conversion mechanism is far away from the optical path of the third reflected light.
The optical path conversion device according to claim 21, wherein the conversion driving assembly comprises a motor and a connecting rod, the motor is connected to the first conversion mechanism through the connecting rod, or the conversion driving assembly It includes a frame device and a stepping motor fixed on the frame device. The stepping motor is connected with the first conversion mechanism through the connecting rod, and is used to drive the first conversion mechanism away from the The optical path of the third reflected light is or is close to the optical path of the third reflected light.
The optical path conversion device according to claim 1, wherein the number of objective lenses in the objective lens assembly is one more than the number of the first conversion mechanism, wherein the optical axis of one objective lens is used to coincide with the observation direction, and the other The positions of the objective lenses correspond one-to-one with the first conversion mechanism.
The optical path conversion device according to claim 18, wherein the number of the third objective lenses is at least two, the number of the third conversion mechanisms is at least two, and each of the third objective lenses and each of the The third conversion mechanisms are arranged in a one-to-one correspondence.
The optical path conversion device according to claim 1, wherein the optical path conversion device further comprises an imaging structure, the conversion component is located between the imaging structure and the objective lens component, and the imaging structure is located in the first On the optical path of the outgoing light, it is used to obtain an enlarged image of the sample to be tested.
The optical path conversion device according to claim 25, wherein the optical path conversion device further comprises a frame structure, and the imaging structure is fixed on the frame structure.
The optical path conversion device according to claim 25, wherein the imaging structure is provided with an adaptor lens or an eyepiece structure; and/or

The optical path conversion device further includes a frame structure, and the first conversion mechanism is fixed on the frame structure; and/or

The imaging structure includes a camera and its data connection port, a magnification and reduction mirror, a connector, a mounting portion, and a lens structure. The lens structure is connected by the connecting piece, the lens structure includes a lens and its mounting seat, the mounting portion is disposed on the outer portion of the connecting piece, and the camera structure is fixed to the outside through the mounting portion.
The optical path conversion device according to claim 25, characterized in that, the optical path conversion device further comprises a control device connected to the imaging structure, the control device is used for monitoring the state of the observation field through the imaging structure and controlling the camera structure. The camera structure is used for automatic camera.
An optical system, characterized in that it comprises an illuminating device, an imaging structure and the optical path conversion device according to any one of claims 1 to 24;

The illumination device is used for emitting the incident light to the first objective lens and the second objective lens;

The imaging structure is arranged on the optical path of the second outgoing light.
The optical system according to claim 29, wherein the lighting device comprises a light source and a moving component, the moving component drives the light source to move, and the light source is used to form the incident light.
The optical system according to claim 30, wherein the moving component comprises a light source guide rail, a light source driver and a base; the light source driver and the light source guide rail are arranged on the base, and the light source is arranged on the light source guide rail In the above, the light source driver is connected to the light source, and the light source driver is used to drive the light source to slide on the light source guide rail, so that the condensing and outgoing direction of the light source coincides with the optical axis of the selected objective lens. .
The optical system according to claim 29, wherein the condensing and outgoing direction of the illumination device is arranged parallel to the optical axis of each objective lens of the objective lens assembly, and the illumination device is arranged in a translation direction toward the objective lens assembly In order to make the condensing and outgoing direction of the illuminating device coincide with the optical axis of the selected objective lens.
The optical system according to claim 29, wherein the optical system further comprises a support platform, the camera structure is fixed on a frame structure, and the frame structure is fixed on the support platform.
The optical system according to claim 29, wherein the optical system further comprises a loading device, the loading device comprises a stage and a power unit, and the stage is arranged on the light of the incident light On the road and used to carry the sample to be tested, the power unit is connected with the stage, and the power unit is used to drive the stage to move to move the sample to be tested to the first objective lens and the second in the field of view of the objective lens.
The optical system according to claim 29, wherein the optical system further comprises a loading device, the loading device is used as a sample platform, and a plurality of samples to be tested are sequentially arranged on the loading device.