WO2022110093A1 - Microbial pesticide formulation for plant protection and growth regulation - Google Patents
Microbial pesticide formulation for plant protection and growth regulation Download PDFInfo
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- WO2022110093A1 WO2022110093A1 PCT/CN2020/132558 CN2020132558W WO2022110093A1 WO 2022110093 A1 WO2022110093 A1 WO 2022110093A1 CN 2020132558 W CN2020132558 W CN 2020132558W WO 2022110093 A1 WO2022110093 A1 WO 2022110093A1
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- Prior art keywords
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- microbial pesticide
- plant protection
- pesticide formulation
- growth regulation
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 43
- 239000003090 pesticide formulation Substances 0.000 title claims abstract description 30
- 230000026267 regulation of growth Effects 0.000 title claims abstract description 24
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 51
- 241000196324 Embryophyta Species 0.000 claims abstract description 32
- 239000000843 powder Substances 0.000 claims abstract description 26
- 150000001413 amino acids Chemical class 0.000 claims abstract description 23
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 20
- 150000002910 rare earth metals Chemical class 0.000 claims abstract description 20
- 229930003231 vitamin Natural products 0.000 claims abstract description 15
- 235000013343 vitamin Nutrition 0.000 claims abstract description 15
- 239000011782 vitamin Substances 0.000 claims abstract description 15
- 229940088594 vitamin Drugs 0.000 claims abstract description 15
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 13
- 235000010755 mineral Nutrition 0.000 claims abstract description 13
- 239000011707 mineral Substances 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 4
- 239000000575 pesticide Substances 0.000 claims description 24
- 235000001014 amino acid Nutrition 0.000 claims description 21
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 20
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 14
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 11
- 239000004472 Lysine Substances 0.000 claims description 11
- BSKHPKMHTQYZBB-UHFFFAOYSA-N 2-methylpyridine Chemical compound CC1=CC=CC=N1 BSKHPKMHTQYZBB-UHFFFAOYSA-N 0.000 claims description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 10
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 10
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 10
- 239000004473 Threonine Substances 0.000 claims description 10
- 229930003268 Vitamin C Natural products 0.000 claims description 10
- 235000004279 alanine Nutrition 0.000 claims description 10
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 10
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 10
- 235000019154 vitamin C Nutrition 0.000 claims description 10
- 239000011718 vitamin C Substances 0.000 claims description 10
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 9
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 9
- 229930003270 Vitamin B Natural products 0.000 claims description 9
- 239000004474 valine Substances 0.000 claims description 9
- 235000019156 vitamin B Nutrition 0.000 claims description 9
- 239000011720 vitamin B Substances 0.000 claims description 9
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 8
- 241000194103 Bacillus pumilus Species 0.000 claims description 7
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 7
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 7
- 241000193386 Lysinibacillus sphaericus Species 0.000 claims description 7
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 7
- 229930182817 methionine Natural products 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 2
- 235000011132 calcium sulphate Nutrition 0.000 claims description 2
- 235000002639 sodium chloride Nutrition 0.000 claims description 2
- 241001131796 Botaurus stellaris Species 0.000 claims 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 1
- 239000005864 Sulphur Substances 0.000 claims 1
- 235000001484 Trigonella foenum graecum Nutrition 0.000 claims 1
- 244000250129 Trigonella foenum graecum Species 0.000 claims 1
- 235000001019 trigonella foenum-graecum Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 14
- 208000035240 Disease Resistance Diseases 0.000 abstract description 8
- 230000001965 increasing effect Effects 0.000 abstract description 7
- 230000006806 disease prevention Effects 0.000 abstract description 5
- 230000002045 lasting effect Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 244000063299 Bacillus subtilis Species 0.000 abstract description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract description 3
- 239000013543 active substance Substances 0.000 abstract description 3
- 230000000443 biocontrol Effects 0.000 abstract description 3
- 230000004060 metabolic process Effects 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 2
- 241000296609 Celastrus angulatus Species 0.000 abstract 2
- 229930195482 Validamycin Natural products 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- JARYYMUOCXVXNK-IMTORBKUSA-N validamycin Chemical compound N([C@H]1C[C@@H]([C@H]([C@H](O)[C@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)CO)[C@H]1C=C(CO)[C@H](O)[C@H](O)[C@H]1O JARYYMUOCXVXNK-IMTORBKUSA-N 0.000 abstract 2
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 7
- 239000000047 product Substances 0.000 description 6
- 235000002566 Capsicum Nutrition 0.000 description 4
- 239000006002 Pepper Substances 0.000 description 4
- 241000722363 Piper Species 0.000 description 4
- 235000016761 Piper aduncum Nutrition 0.000 description 4
- 235000017804 Piper guineense Nutrition 0.000 description 4
- 235000008184 Piper nigrum Nutrition 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000000855 fungicidal effect Effects 0.000 description 4
- 239000000417 fungicide Substances 0.000 description 4
- 229920001491 Lentinan Polymers 0.000 description 3
- 229940115286 lentinan Drugs 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000013586 microbial product Substances 0.000 description 3
- 244000144730 Amygdalus persica Species 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 235000018262 Arachis monticola Nutrition 0.000 description 2
- 244000003416 Asparagus officinalis Species 0.000 description 2
- 235000005340 Asparagus officinalis Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
- 241000219146 Gossypium Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 241000220225 Malus Species 0.000 description 2
- 235000011430 Malus pumila Nutrition 0.000 description 2
- 235000015103 Malus silvestris Nutrition 0.000 description 2
- 235000006040 Prunus persica var persica Nutrition 0.000 description 2
- 235000014443 Pyrus communis Nutrition 0.000 description 2
- 240000001987 Pyrus communis Species 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 244000061458 Solanum melongena Species 0.000 description 2
- 235000002597 Solanum melongena Nutrition 0.000 description 2
- 244000126002 Ziziphus vulgaris Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 235000019743 Choline chloride Nutrition 0.000 description 1
- 241000065610 Cotinus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000005980 Gibberellic acid Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000745988 Phyllostachys Species 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960003178 choline chloride Drugs 0.000 description 1
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
- 239000004476 plant protection product Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
Definitions
- the invention relates to a microbial pesticide preparation, in particular to a microbial pesticide preparation for plant protection growth regulation.
- plants including all kinds of crops, will be constrained by various adversity factors in their life, such as cold, high temperature, drought, salinity, waterlogging, toxins, etc., coupled with the influence of diseases and insect pests, thus restricting the Increased crop yield and improved quality.
- PLM plant health management
- the Chinese invention patent with the application number of CN201510998185.4 discloses a biological pesticide composition and application for preventing and treating Cotinus wilt. Specifically, it includes the use of three different biological pesticides at different growth stages, and the biological pesticide a includes 13-18 parts of plant fungicide a, 4-6 parts of phylloxacin, and 12-15 parts of microbial product fungicide a; Biopesticide b includes 12-20 parts of plant fungicide b, 1-4 parts of gibberellic acid, 3-5 parts of woodnicotine, 12-16 parts of microbial product fungicide b, and 30-40 parts of water; biological pesticides c includes 15-20 parts of plant bactericide c, 3-6 parts of Bacillus sp., 1-5 parts of oilseed oil, 3-6 parts of thaliana, and 10-15 parts of microbial product bactericide c by mass.
- Such biological pesticides are used in a large amount, the use process is cumbersome, and the cost is high.
- the Chinese invention patent with the application number of CN200910248521.8 discloses a new type of biological pesticide compounded by Jinggangmycin and lentinan, the formula and the weight ratio thereof are as follows: 0.25-0.48 part of lentinan, 0.5-2.5 part of Jinggangmycin , 1 to 1.5 parts of amino acids, and 95.52 to 98.25 parts of water.
- the lentinan used in the biological pesticide has high cost.
- the Chinese invention patent with the application number of CN201110352113.4 discloses a microbial pesticide preparation for plant protection growth regulation, including 0.5-5% of Wuyi fungus spore powder, 5-25% of choline chloride, light rare earth 10% -30%, methionine 5-20%, zinc sulfate 1-8%, copper sulfate 0.3-3%, boric acid 1-5%, vitamin B0.05-0.5%, vitamin C0.05-0.5%, filler kaolin quantity.
- a low-cost microbial pesticide formulation is expected, which has obvious disease prevention, disease resistance, stress resistance, increased crop yield, improved crop product quality, and has obvious yield-increasing effect, lasting effect, stable control effect, and low cost.
- the production process is simple.
- the object of the present invention is to provide a low-cost microbial pesticide formulation, which has obvious disease prevention, disease resistance, stress resistance, increase crop yield, and improve crop product quality, and has obvious yield increasing effect, lasting effect, and control effect. Stable, low cost and simple production process.
- the invention provides a microbial pesticide preparation for plant protection growth regulation, which is characterized in that it is composed of the following raw materials in percentage by weight: Bacillus sp. spore powder 1-5%, ethyl acetate 10-20%, amino acid 10-5% 20%, Jinggangmycin 5-10%, Phyllostachys 1-5%, Light Rare Earth 40-60%.
- the aforementioned microbial pesticide preparation for plant protection growth regulation wherein, it is composed of the following raw materials by weight: 3% of Bacillus sp. spore powder, 15% of ethyl acetate, 20% of amino acids, 7% of Jinggangmycin, 5% element, 50% light rare earth.
- the aforementioned microbial pesticide preparation for plant protection growth regulation wherein, it is composed of the following raw materials by weight: 3% of Bacillus spore powder, 20% of ethyl acetate, 18% of amino acids, 7% of Jinggangmycin, 2% element, 50% light rare earth.
- Bacillus sp. spore powder is Bacillus pumilus spore powder or Bacillus sphaericus spore powder.
- the spore content of the Bacillus spore powder is 2 billion/g.
- the aforementioned microbial pesticide formulation for plant protection growth regulation wherein, the amino acid is selected from any one or two or more of alanine, lysine, methionine, valine, and threonine. mix.
- the aforementioned microbial pesticide formulation for plant protection growth regulation further comprises vitamins.
- the vitamins include vitamin B and vitamin C.
- the aforementioned microbial pesticide formulation for plant protection growth regulation further includes minerals.
- the minerals include calcium sulfate, magnesium sulfate, sodium chloride, potassium chloride, ferric chloride, and copper sulfate.
- the microbial pesticide preparation of the invention uses Bacillus subtilis as the main active substance, and is mixed with amino acids at the same time, and light rare earth provides nutrients required for growth and metabolism of the colony, which can improve the activity of the bacteria. It can form a functional biocontrol bacterial combination with obvious disease resistance, stress resistance, yield increase and optimization of agricultural product quality.
- the preparation of the invention can be widely used in vegetables such as cucumber, pepper, eggplant, tomato, watermelon, fruit trees such as winter jujube, apple, peach, pear, economic crops such as asparagus, peanut, cotton, etc., and forest flowers and plants.
- the preparation of the present invention is convenient to use, and can be powdered for hole application, spread application, or diluted into a suspension for spray application.
- the pesticide preparation of the invention has obvious disease prevention, disease resistance, stress resistance, increases crop yield, and improves crop product quality, and has obvious yield increasing effect, lasting effect, stable control effect, low cost, simple production process, and can be directly mixed according to the components. That is, no waste pollution.
- Bacillus sphaericus spore powder (the spore content is 2 billion/g), 20 parts of ethyl acetate, 18 parts of amino acids (10 parts of lysine, 4 parts of methionine, 4 parts of methionine) Threonine), 7 parts of Jinggangmycin, 2 parts of picolin and 40 parts of light rare earth, 5 parts of vitamins and 5 parts of minerals (2 parts of magnesium sulfate, 2 parts of sodium chloride, 1 part of copper sulfate) and 1000 parts
- the microbial pesticide formulations are prepared by mixing and stirring evenly.
- 3 parts by weight of Bacillus pumilus spore powder (the spore content is 2 billion/g), 20 parts of ethyl acetate, 10 parts of amino acids (1 part of alanine, 4 parts of lysine, 1 part of methyl alcohol) thionine, 1 part valine, 3 parts threonine), 7 parts Jinggangmycin, 5 parts picolin, 50 parts light rare earth, 6 parts vitamins (3 parts vitamin B, 3 parts vitamin C) and 9 parts of minerals (1 part of calcium sulfate, 2 parts of magnesium sulfate, 2 parts of sodium chloride, 2 parts of potassium chloride, 1 part of ferric chloride, 1 part of copper sulfate) are mixed with 1000 parts of water and stirred well to obtain Microbial pesticide formulations.
- Bacillus pumilus spore powder (the spore content is 2 billion per gram), 20 parts of ethyl acetate, 10 parts of amino acids (2 parts of alanine, 2 parts of lysine, 2 parts of methyl alcohol) thionine, 1 part valine, 3 parts threonine), 10 parts Jinggangmycin, 5 parts picolin, 40 parts light rare earth, 6 parts vitamins (4 parts vitamin B, 2 parts vitamin C) and 4 parts of minerals (1 part of calcium sulfate, 1 part of magnesium sulfate, 1 part of ferric chloride, 1 part of copper sulfate) are mixed with 1000 parts of water and stirred evenly to prepare a microbial pesticide formulation.
- Bacillus pumilus spore powder (the spore content is 2 billion per gram), 10 parts of ethyl acetate, 20 parts of amino acids (4 parts of alanine, 4 parts of lysine, 4 parts of methyl alcohol) thionine, 4 parts valine, 4 parts threonine), 5 parts Jinggangmycin, 5 parts picolin, 40 parts light rare earth, 10 parts vitamins (3 parts vitamin B, 7 parts vitamin C) and 5 parts of minerals (2 parts of potassium chloride, 1 part of ferric chloride, 2 parts of copper sulfate) and 1000 parts of water are mixed and stirred evenly to prepare a microbial pesticide formulation.
- the microbial pesticide formulations of Examples 1 to 10 of the present invention were used for the prevention and treatment of pepper blight, and the results were shown in Table 1 below according to the conventional usage method and dosage.
- Example 1 97 98
- Example 2 95 99
- Example 3 96
- Example 4 89
- Example 5 93
- Example 6 91
- Example 7 92
- Example 8 94
- Example 9 97
- Example 10 88 95
- the microbial pesticide formulation of the present invention has an obvious control effect on pepper blight, and is better than conventional pesticides.
- the microbial pesticide formulation of the present invention uses Bacillus subtilis as the main active substance, and is supplemented with amino acids and light rare earths to provide nutrients required for growth and metabolism of the colony, which can improve the activity of the bacteria. It can form a functional bio-control bacterial combination with obvious disease resistance, stress resistance, yield increase and optimization of agricultural product quality.
- the preparation of the invention can be widely used in vegetables such as cucumber, pepper, eggplant, tomato, watermelon, fruit trees such as winter jujube, apple, peach, pear, economic crops such as asparagus, peanut, cotton, etc., and forest flowers and plants.
- the preparation of the present invention is convenient to use, and can be powdered for hole application, spread application, or diluted into a suspension for spray application.
- the pesticide preparation of the invention has obvious disease prevention, disease resistance, stress resistance, increases crop yield, and improves crop product quality, and has obvious yield increasing effect, lasting effect, stable control effect, low cost, simple production process, and can be directly mixed according to the components. That is, no waste pollution.
Abstract
Disclosed is a microbial pesticide formulation for plant protection and growth regulation. The microbial pesticide formulation consists of the following raw materials in percentage by weight: 1-5% of bacillus spore powder, 10-20% of ethyl acetate, 10-20% of an amino acid, 5-10% of validamycin, 1-5% of celastrus angulatus, and 40-60% of light rare earth. The disclosed microbial pesticide formulation uses bacillus subtilis as a main active substance and is compounded with the amino acid and the light rare earth so as to provide nutrient substances required for growth metabolism for colonies, so that the activity of a strain can be improved, and a biocontrol composition having the functions of having obvious disease resistance, stress resistance, and yield increasing, and optimizing the quality of agricultural products can be formed by means of the cooperation of substances, such as validamycin, celastrus angulatus, vitamins, and minerals. The disclosed pesticide formulation has obvious disease prevention, disease resistance, and stress resistance, increases the crop yield, improves the quality of the crop products, and is obvious in yield increasing effect, lasting in effect, stable in prevention effect, low in cost, and simple in production process. The components can be directly mixed, and there is no waste pollution.
Description
本发明涉及微生物农药制剂，具体涉及一种用于植物保护生长调节的微生物农药制剂。The invention relates to a microbial pesticide preparation, in particular to a microbial pesticide preparation for plant protection growth regulation.
传统的植物保护理念侧重于利用各种手段针对植物的病虫害进行杀灭、抑制。各种预防性的措施往往也是针对不同的病虫害而进行的。然而这在一定程度上提高了病虫害的抗性，从而导致植物保护产品的施用浓度越来越高，对环境的影响也愈加沉重，其防治效果也更不理想。The traditional concept of plant protection focuses on the use of various means to kill and suppress plant diseases and insect pests. Various preventive measures are often carried out for different pests and diseases. However, this improves the resistance of pests and diseases to a certain extent, which leads to higher and higher application concentrations of plant protection products, which has a heavier impact on the environment and less ideal control effects.
其实植物包括各种农作物在种植时一生均会受到各种不同程度的逆境因素的制约，例如寒冷、高温、干旱、盐碱、水涝、毒素等影响，再加上病虫害的影响，从而制约了作物产量的提高和品质的改善。国际学术界近年提出了植物健康操控(plant health management，PHM)理论，就是要研究如何提高作物适应逆境因素的能力和减轻逆境因素对作物生长的影响，调动作物自身的抗逆、免疫功能，是提高作物产量和品质的有效途径。In fact, plants, including all kinds of crops, will be constrained by various adversity factors in their life, such as cold, high temperature, drought, salinity, waterlogging, toxins, etc., coupled with the influence of diseases and insect pests, thus restricting the Increased crop yield and improved quality. In recent years, the international academic community has put forward the theory of plant health management (PHM), which is to study how to improve the ability of crops to adapt to adversity factors and reduce the impact of adversity factors on crop growth, so as to mobilize the resistance and immune functions of animals. An effective way to improve crop yield and quality.
例如，申请号为CN201510998185.4的中国发明专利公开了一种防治黄栌枯萎病的生物农药组合物及应用。具体包括在不同的生长阶段使用三种不同的生物农药，生物农药a以质量计包括植物杀菌剂a 13-18份、苦皮藤素4-6份、微生物产物杀菌剂a 12-15份；生物农药b以质量计包括植物杀菌剂b 12-20份、赤霉酸1-4份、木烟碱3-5份、微生物产物杀菌剂b 12-16份，水30-40份；生物农药c以质量计包括植物杀菌剂c 15-20份、芽孢杆菌菌属3-6份、油籽油1-5份、百部碱3-6份、微生物产物杀菌剂c 10-15份。该类生物农药使用量较多，使用过程繁琐，且成本高。For example, the Chinese invention patent with the application number of CN201510998185.4 discloses a biological pesticide composition and application for preventing and treating Cotinus wilt. Specifically, it includes the use of three different biological pesticides at different growth stages, and the biological pesticide a includes 13-18 parts of plant fungicide a, 4-6 parts of phylloxacin, and 12-15 parts of microbial product fungicide a; Biopesticide b includes 12-20 parts of plant fungicide b, 1-4 parts of gibberellic acid, 3-5 parts of woodnicotine, 12-16 parts of microbial product fungicide b, and 30-40 parts of water; biological pesticides c includes 15-20 parts of plant bactericide c, 3-6 parts of Bacillus sp., 1-5 parts of oilseed oil, 3-6 parts of thaliana, and 10-15 parts of microbial product bactericide c by mass. Such biological pesticides are used in a large amount, the use process is cumbersome, and the cost is high.
又如，申请号为CN200910248521.8的中国发明专利公开了一种井冈霉素和香菇多糖复配新型生物农药，配方及其重量比如下：香菇多糖0.25～0.48份，井冈霉素0.5～2.5份，氨基酸1～1.5份，水95.52～98.25份。该生物农药所用的香菇多糖成本高。Another example, the Chinese invention patent with the application number of CN200910248521.8 discloses a new type of biological pesticide compounded by Jinggangmycin and lentinan, the formula and the weight ratio thereof are as follows: 0.25-0.48 part of lentinan, 0.5-2.5 part of Jinggangmycin , 1 to 1.5 parts of amino acids, and 95.52 to 98.25 parts of water. The lentinan used in the biological pesticide has high cost.
又如，申请号为CN201110352113.4的中国发明专利公开了一种用于植物保护生长调节的微生物农药制剂，包括武夷菌孢子粉0.5-5％，氯化胆碱5-25％、轻稀土10-30％、蛋氨酸5-20％、硫酸锌1-8％、硫酸铜0.3-3％、硼酸1-5％、维生素B0.05-0.5％、维生素C0.05-0.5％、填充料高岭土余量。Another example, the Chinese invention patent with the application number of CN201110352113.4 discloses a microbial pesticide preparation for plant protection growth regulation, including 0.5-5% of Wuyi fungus spore powder, 5-25% of choline chloride, light rare earth 10% -30%, methionine 5-20%, zinc sulfate 1-8%, copper sulfate 0.3-3%, boric acid 1-5%, vitamin B0.05-0.5%, vitamin C0.05-0.5%, filler kaolin quantity.
基于以上，期待一种成本较低的微生物农药制剂，具有明显的防病、抗病、抗逆、提高农作物产量、改善农作物产品品质，且增产效果明显，效果持久，防效稳定，成本低，生产工艺简单。Based on the above, a low-cost microbial pesticide formulation is expected, which has obvious disease prevention, disease resistance, stress resistance, increased crop yield, improved crop product quality, and has obvious yield-increasing effect, lasting effect, stable control effect, and low cost. The production process is simple.
发明内容SUMMARY OF THE INVENTION
鉴于此，本发明的目的是提供一种成本较低的微生物农药制剂，具有明显的防病、抗病、抗逆、提高农作物产量、改善农作物产品品质，且增产效果明显，效果持久，防效稳定，成本低，生产工艺简单。In view of this, the object of the present invention is to provide a low-cost microbial pesticide formulation, which has obvious disease prevention, disease resistance, stress resistance, increase crop yield, and improve crop product quality, and has obvious yield increasing effect, lasting effect, and control effect. Stable, low cost and simple production process.
本发明的目的是通过以下技术方案来实现的：The purpose of this invention is to realize through the following technical solutions:
本发明提供了一种用于植物保护生长调节的微生物农药制剂，其特征在于，由以下重量百分比原料组成：芽孢杆菌菌属孢子粉1～5％、乙酸乙酯10～20％、氨基酸10～20％、井冈霉素5～10％、苦皮藤素1～5％、轻稀土40～60％。The invention provides a microbial pesticide preparation for plant protection growth regulation, which is characterized in that it is composed of the following raw materials in percentage by weight: Bacillus sp. spore powder 1-5%, ethyl acetate 10-20%, amino acid 10-5% 20%, Jinggangmycin 5-10%, Phyllostachys 1-5%, Light Rare Earth 40-60%.
前述的用于植物保护生长调节的微生物农药制剂，其中，由以下重量百分比原料组成：芽孢杆菌菌属孢子粉3％、乙酸乙酯15％、氨基酸20％、井冈霉素7％、苦皮藤素5％、轻稀土50％。The aforementioned microbial pesticide preparation for plant protection growth regulation, wherein, it is composed of the following raw materials by weight: 3% of Bacillus sp. spore powder, 15% of ethyl acetate, 20% of amino acids, 7% of Jinggangmycin, 5% element, 50% light rare earth.
前述的用于植物保护生长调节的微生物农药制剂，其中，由以下重量百分比原料组成：芽孢杆菌菌属孢子粉3％、乙酸乙酯20％、氨基酸18％、井冈霉素7％、苦皮藤素2％、轻稀土50％。The aforementioned microbial pesticide preparation for plant protection growth regulation, wherein, it is composed of the following raw materials by weight: 3% of Bacillus spore powder, 20% of ethyl acetate, 18% of amino acids, 7% of Jinggangmycin, 2% element, 50% light rare earth.
前述的用于植物保护生长调节的微生物农药制剂，其中，所述芽孢杆菌菌属孢子粉为短小芽孢杆菌孢子粉或球形芽孢杆菌孢子粉。The aforementioned microbial pesticide formulation for plant protection growth regulation, wherein the Bacillus sp. spore powder is Bacillus pumilus spore powder or Bacillus sphaericus spore powder.
前述的用于植物保护生长调节的微生物农药制剂，其中，所述芽孢杆菌菌属孢子粉的孢子含量为20亿个/克。In the aforementioned microbial pesticide formulation for plant protection growth regulation, the spore content of the Bacillus spore powder is 2 billion/g.
前述的用于植物保护生长调节的微生物农药制剂，其中，所述氨基酸选自丙氨酸、赖氨酸、甲硫氨酸、缬氨酸、苏氨酸中的任一种或两种以上的混合。The aforementioned microbial pesticide formulation for plant protection growth regulation, wherein, the amino acid is selected from any one or two or more of alanine, lysine, methionine, valine, and threonine. mix.
前述的用于植物保护生长调节的微生物农药制剂，其中，还包含维生素。The aforementioned microbial pesticide formulation for plant protection growth regulation further comprises vitamins.
前述的用于植物保护生长调节的微生物农药制剂，其中，所述维生素包括维生素B、维生素C。In the aforementioned microbial pesticide formulation for plant protection growth regulation, wherein the vitamins include vitamin B and vitamin C.
前述的用于植物保护生长调节的微生物农药制剂，其中，还包括矿物质。The aforementioned microbial pesticide formulation for plant protection growth regulation further includes minerals.
前述的用于植物保护生长调节的微生物农药制剂，其中，所述矿物质包括硫酸钙、硫酸镁、氯化钠、氯化钾、氯化铁、硫酸铜。In the aforementioned microbial pesticide formulation for plant protection growth regulation, wherein, the minerals include calcium sulfate, magnesium sulfate, sodium chloride, potassium chloride, ferric chloride, and copper sulfate.
本发明有益效果：Beneficial effects of the present invention:
本发明的微生物农药制剂以枯草芽孢杆菌属为主要活性物质，同时配以氨基酸，轻稀土为菌落提供生长代谢所需的营养物质，能够提高菌种的活性，再配合井冈霉素、苦皮藤素、 维生素、矿物质等物质，可形成具有明显抗病、抗逆、增产和优化农产品品质的功能生防菌组合。本发明制剂能够广泛应用于如黄瓜、辣椒、茄子、番茄、西瓜等蔬菜、如冬枣、苹果、桃、梨等果树、如芦笋、花生、棉花等经济作物以及林本花卉植物等。另外，本发明制剂使用方便，可粉状穴施、撒施，也可稀释为悬浮液喷洒施用。本发明的农药制剂具有明显的防病、抗病、抗逆、提高农作物产量、改善农作物产品品质，且增产效果明显，效果持久，防效稳定，成本低，生产工艺简单，按照组分直接混合即可，无废弃物污染。The microbial pesticide preparation of the invention uses Bacillus subtilis as the main active substance, and is mixed with amino acids at the same time, and light rare earth provides nutrients required for growth and metabolism of the colony, which can improve the activity of the bacteria. It can form a functional biocontrol bacterial combination with obvious disease resistance, stress resistance, yield increase and optimization of agricultural product quality. The preparation of the invention can be widely used in vegetables such as cucumber, pepper, eggplant, tomato, watermelon, fruit trees such as winter jujube, apple, peach, pear, economic crops such as asparagus, peanut, cotton, etc., and forest flowers and plants. In addition, the preparation of the present invention is convenient to use, and can be powdered for hole application, spread application, or diluted into a suspension for spray application. The pesticide preparation of the invention has obvious disease prevention, disease resistance, stress resistance, increases crop yield, and improves crop product quality, and has obvious yield increasing effect, lasting effect, stable control effect, low cost, simple production process, and can be directly mixed according to the components. That is, no waste pollution.
为了进一步理解本发明，下面结合实施例对本发明优选实施方案进行描述，但是应当理解，这些描述只是为进一步说明本发明的特征和优点，而不是对本发明权利要求的限制，本发明试剂如无特别说明，均为常规试剂，均可由市场购买得到。In order to further understand the present invention, the preferred embodiments of the present invention are described below in conjunction with the examples, but it should be understood that these descriptions are only for further illustrating the features and advantages of the present invention, rather than limiting the claims of the present invention. It is indicated that they are all conventional reagents, which can be purchased from the market.
实施例1Example 1
将重量份为3份的球形芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、15份乙酸乙酯、20份氨基酸(4份丙氨酸、4份赖氨酸、4份甲硫氨酸、4份缬氨酸、4份苏氨酸)、7份井冈霉素、5份苦皮藤素以及50份轻稀土与1000份水混合并搅拌均匀，即制得微生物农药制剂。3 parts by weight of Bacillus sphaericus spore powder (the spore content is 2 billion per gram), 15 parts of ethyl acetate, 20 parts of amino acids (4 parts of alanine, 4 parts of lysine, 4 parts of methyl alcohol) thionine, 4 parts of valine, 4 parts of threonine), 7 parts of Jinggangmycin, 5 parts of picolin and 50 parts of light rare earth are mixed with 1000 parts of water and stirred evenly to prepare a microbial pesticide preparation.
实施例2Example 2
将重量份为3份的球形芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、20份乙酸乙酯、18份氨基酸(6份丙氨酸、4份赖氨酸、4份甲硫氨酸、4份缬氨酸)、7份井冈霉素、2份苦皮藤素以及50份轻稀土与1000份水混合并搅拌均匀，即制得微生物农药制剂。3 parts by weight of Bacillus sphaericus spore powder (the spore content is 2 billion/g), 20 parts of ethyl acetate, 18 parts of amino acids (6 parts of alanine, 4 parts of lysine, 4 parts of methyl alcohol) thionine, 4 parts of valine), 7 parts of Jinggangmycin, 2 parts of picrophyllin and 50 parts of light rare earth are mixed with 1000 parts of water and stirred evenly to prepare a microbial pesticide preparation.
实施例3Example 3
将重量份为3份的球形芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、20份乙酸乙酯、18份氨基酸(10份赖氨酸、4份甲硫氨酸、4份苏氨酸)、7份井冈霉素、2份苦皮藤素以及40份轻稀土、5份维生素以及5份矿物质(2份硫酸镁、2份氯化钠、1份硫酸铜)与1000份水混合并搅拌均匀，即制得微生物农药制剂。3 parts by weight of Bacillus sphaericus spore powder (the spore content is 2 billion/g), 20 parts of ethyl acetate, 18 parts of amino acids (10 parts of lysine, 4 parts of methionine, 4 parts of methionine) Threonine), 7 parts of Jinggangmycin, 2 parts of picolin and 40 parts of light rare earth, 5 parts of vitamins and 5 parts of minerals (2 parts of magnesium sulfate, 2 parts of sodium chloride, 1 part of copper sulfate) and 1000 parts The microbial pesticide formulations are prepared by mixing and stirring evenly.
实施例4Example 4
将重量份为3份的球形芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、20份乙酸乙酯、18份氨基酸(4份丙氨酸、6份赖氨酸、1份甲硫氨酸、7份缬氨酸)、7份井冈霉素、2份苦皮藤素以及45份轻稀土、2份维生素(1份维生素B、1份维生素C)以及3份矿物质(1份硫酸钙、1份氯化铁、1份硫酸铜)与1000份水混合并搅拌均匀，即制得微生物农药制剂。3 parts by weight of Bacillus sphaericus spore powder (the spore content is 2 billion/g), 20 parts of ethyl acetate, 18 parts of amino acids (4 parts of alanine, 6 parts of lysine, 1 part of formazan) thionine, 7 parts valine), 7 parts Jinggangmycin, 2 parts picolin and 45 parts light rare earth, 2 parts vitamins (1 part vitamin B, 1 part vitamin C) and 3 parts minerals (1 part vitamin C) 1 part of calcium sulfate, 1 part of ferric chloride, 1 part of copper sulfate) and 1000 parts of water were mixed and stirred evenly to prepare a microbial pesticide preparation.
实施例5Example 5
将重量份为3份的球形芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、15份乙酸乙酯、20份氨基酸(5份赖氨酸、5份甲硫氨酸、5份缬氨酸、5份苏氨酸)、7份井冈霉素、5份苦皮藤素、40份轻稀土、6份维生素(1份维生素B、5份维生素C)以及4份矿物质(2份氯化钠、1份氯化铁、1份硫酸铜)与1000份水混合并搅拌均匀，即制得微生物农药制剂。3 parts by weight of Bacillus sphaericus spore powder (the spore content is 2 billion/g), 15 parts of ethyl acetate, 20 parts of amino acids (5 parts of lysine, 5 parts of methionine, 5 parts of methionine) valine, 5 parts threonine), 7 parts Jinggangmycin, 5 parts picolin, 40 parts light rare earth, 6 parts vitamins (1 part vitamin B, 5 parts vitamin C) and 4 parts minerals (2 parts 1 part of sodium chloride, 1 part of ferric chloride, 1 part of copper sulfate) and 1000 parts of water were mixed and stirred evenly to prepare a microbial pesticide preparation.
实施例6Example 6
将重量份为3份的短小芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、15份乙酸乙酯、10份氨基酸(5份丙氨酸、5份苏氨酸)、7份井冈霉素、5份苦皮藤素、50份轻稀土、6份维生素(5份维生素B、1份维生素C)以及4份矿物质(1份硫酸钙、2份硫酸镁、1份硫酸铜)与1000份水混合并搅拌均匀，即制得微生物农药制剂。3 parts by weight of Bacillus pumilus spore powder (the spore content is 2 billion/g), 15 parts of ethyl acetate, 10 parts of amino acids (5 parts of alanine, 5 parts of threonine), 7 parts of Jinggangmycin, 5 parts of picolin, 50 parts of light rare earth, 6 parts of vitamins (5 parts of vitamin B, 1 part of vitamin C) and 4 parts of minerals (1 part of calcium sulfate, 2 parts of magnesium sulfate, 1 part of copper sulfate ) mixed with 1000 parts of water and stirred evenly to obtain a microbial pesticide formulation.
实施例7Example 7
将重量份为3份的短小芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、20份乙酸乙酯、10份氨基酸(1份丙氨酸、4份赖氨酸、1份甲硫氨酸、1份缬氨酸、3份苏氨酸)、7份井冈霉素、5份苦皮藤素、50份轻稀土、6份维生素(3份维生素B、3份维生素C)以及9份矿物质(1份硫酸钙、2份硫酸镁、2份氯化钠、2份氯化钾、1份氯化铁、1份硫酸铜)与1000份水混合并搅拌均匀，即制得微生物农药制剂。3 parts by weight of Bacillus pumilus spore powder (the spore content is 2 billion/g), 20 parts of ethyl acetate, 10 parts of amino acids (1 part of alanine, 4 parts of lysine, 1 part of methyl alcohol) thionine, 1 part valine, 3 parts threonine), 7 parts Jinggangmycin, 5 parts picolin, 50 parts light rare earth, 6 parts vitamins (3 parts vitamin B, 3 parts vitamin C) and 9 parts of minerals (1 part of calcium sulfate, 2 parts of magnesium sulfate, 2 parts of sodium chloride, 2 parts of potassium chloride, 1 part of ferric chloride, 1 part of copper sulfate) are mixed with 1000 parts of water and stirred well to obtain Microbial pesticide formulations.
实施例8Example 8
将重量份为5份的短小芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、20份乙酸乙酯、10份氨基酸(2份丙氨酸、2份赖氨酸、2份甲硫氨酸、1份缬氨酸、3份苏氨酸)、10份井冈霉素、5份苦皮藤素、40份轻稀土、6份维生素(4份维生素B、2份维生素C)以及4份矿物质(1份硫酸钙、1份硫酸镁、1份氯化铁、1份硫酸铜)与1000份水混合并搅拌均匀，即制得微生物农药制剂。5 parts by weight of Bacillus pumilus spore powder (the spore content is 2 billion per gram), 20 parts of ethyl acetate, 10 parts of amino acids (2 parts of alanine, 2 parts of lysine, 2 parts of methyl alcohol) thionine, 1 part valine, 3 parts threonine), 10 parts Jinggangmycin, 5 parts picolin, 40 parts light rare earth, 6 parts vitamins (4 parts vitamin B, 2 parts vitamin C) and 4 parts of minerals (1 part of calcium sulfate, 1 part of magnesium sulfate, 1 part of ferric chloride, 1 part of copper sulfate) are mixed with 1000 parts of water and stirred evenly to prepare a microbial pesticide formulation.
实施例9Example 9
将重量份为5份的短小芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、10份乙酸乙酯、20份氨基酸(4份丙氨酸、6份赖氨酸、4份甲硫氨酸、6份苏氨酸)、5份井冈霉素、5份苦皮藤素、45份轻稀土、6份维生素(2份维生素B、4份维生素C)以及4份矿物质(1份硫酸钙、1份硫酸镁、1份氯化钠、1份氯化钾)与1000份水混合并搅拌均匀，即制得微生物农药制剂。。5 parts by weight of Bacillus pumilus spore powder (the spore content is 2 billion/g), 10 parts of ethyl acetate, 20 parts of amino acids (4 parts of alanine, 6 parts of lysine, 4 parts of methyl alcohol) thionine, 6 parts threonine), 5 parts Jinggangmycin, 5 parts picolin, 45 parts light rare earth, 6 parts vitamins (2 parts vitamin B, 4 parts vitamin C) and 4 parts minerals (1 1 part of calcium sulfate, 1 part of magnesium sulfate, 1 part of sodium chloride, 1 part of potassium chloride) and 1000 parts of water are mixed and stirred evenly to prepare a microbial pesticide preparation. .
实施例10Example 10
将重量份为5份的短小芽孢杆菌菌属孢子粉(孢子含量为20亿个/克)、10份乙酸乙酯、20份氨基酸(4份丙氨酸、4份赖氨酸、4份甲硫氨酸、4份缬氨酸、4份苏氨酸)、5份井 冈霉素、5份苦皮藤素、40份轻稀土、10份维生素(3份维生素B、7份维生素C)以及5份矿物质(2份氯化钾、1份氯化铁、2份硫酸铜)与1000份水混合并搅拌均匀，即制得微生物农药制剂。5 parts by weight of Bacillus pumilus spore powder (the spore content is 2 billion per gram), 10 parts of ethyl acetate, 20 parts of amino acids (4 parts of alanine, 4 parts of lysine, 4 parts of methyl alcohol) thionine, 4 parts valine, 4 parts threonine), 5 parts Jinggangmycin, 5 parts picolin, 40 parts light rare earth, 10 parts vitamins (3 parts vitamin B, 7 parts vitamin C) and 5 parts of minerals (2 parts of potassium chloride, 1 part of ferric chloride, 2 parts of copper sulfate) and 1000 parts of water are mixed and stirred evenly to prepare a microbial pesticide formulation.
试验例Test example
使用本发明实施例1～10的微生物农药制剂用于辣椒疫病的防治，按照常规使用方法与用量，结果见下表1。The microbial pesticide formulations of Examples 1 to 10 of the present invention were used for the prevention and treatment of pepper blight, and the results were shown in Table 1 below according to the conventional usage method and dosage.
表1防治效果Table 1 Control effect
| 药剂medicine | 病斑抑制率(％)Lesion Inhibition Rate (%) | 综合防治效果(％)Comprehensive control effect (%) |
| 实施例1Example 1 | 9797 | 9898 |
| 实施例2Example 2 | 9595 | 9999 |
| 实施例3Example 3 | 9696 | 9797 |
| 实施例4Example 4 | 8989 | 9292 |
| 实施例5Example 5 | 9393 | 9090 |
| 实施例6Example 6 | 9191 | 8989 |
| 实施例7Example 7 | 9292 | 9595 |
| 实施例8Example 8 | 9494 | 9191 |
| 实施例9Example 9 | 9797 | 9292 |
| 实施例10Example 10 | 8888 | 9595 |
由表1的结果可以看出，本发明的微生物农药制剂对辣椒疫病的防治效果明显，且较常规的农药效果更好。As can be seen from the results in Table 1, the microbial pesticide formulation of the present invention has an obvious control effect on pepper blight, and is better than conventional pesticides.
综上所述，本发明的微生物农药制剂以枯草芽孢杆菌属为主要活性物质，同时配以氨基酸，轻稀土为菌落提供生长代谢所需的营养物质，能够提高菌种的活性，再配合井冈霉素、苦皮藤素、维生素、矿物质等物质，可形成具有明显抗病、抗逆、增产和优化农产品品质的功能生防菌组合。本发明制剂能够广泛应用于如黄瓜、辣椒、茄子、番茄、西瓜等蔬菜、如冬枣、苹果、桃、梨等果树、如芦笋、花生、棉花等经济作物以及林本花卉植物等。另外，本发明制剂使用方便，可粉状穴施、撒施，也可稀释为悬浮液喷洒施用。本发明的农药制剂具有明显的防病、抗病、抗逆、提高农作物产量、改善农作物产品品质，且增产效果明显，效果持久，防效稳定，成本低，生产工艺简单，按照组分直接混合即可，无废弃物污染。To sum up, the microbial pesticide formulation of the present invention uses Bacillus subtilis as the main active substance, and is supplemented with amino acids and light rare earths to provide nutrients required for growth and metabolism of the colony, which can improve the activity of the bacteria. It can form a functional bio-control bacterial combination with obvious disease resistance, stress resistance, yield increase and optimization of agricultural product quality. The preparation of the invention can be widely used in vegetables such as cucumber, pepper, eggplant, tomato, watermelon, fruit trees such as winter jujube, apple, peach, pear, economic crops such as asparagus, peanut, cotton, etc., and forest flowers and plants. In addition, the preparation of the present invention is convenient to use, and can be powdered for hole application, spread application, or diluted into a suspension for spray application. The pesticide preparation of the invention has obvious disease prevention, disease resistance, stress resistance, increases crop yield, and improves crop product quality, and has obvious yield increasing effect, lasting effect, stable control effect, low cost, simple production process, and can be directly mixed according to the components. That is, no waste pollution.
以上所述仅是本发明的优选实施方式，本发明的保护范围并不仅局限于上述实施例，凡属于本发明思路下的技术方案均属于本发明的保护范围。应当指出，对于本技术领域的普通 技术人员来说，在不脱离本发明原理前提下的若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。The above are only the preferred embodiments of the present invention, and the protection scope of the present invention is not limited to the above-mentioned embodiments, and all technical solutions under the idea of the present invention belong to the protection scope of the present invention. It should be pointed out that for those skilled in the art, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.
Claims (10)
- 一种用于植物保护生长调节的微生物农药制剂，其特征在于，由以下重量百分比原料组成：芽孢杆菌菌属孢子粉1～5％、乙酸乙酯10～20％、氨基酸10～20％、井冈霉素5～10％、苦皮藤素1～5％、轻稀土40～60％。A microbial pesticide preparation for plant protection growth regulation, characterized in that it is composed of the following raw materials in percentage by weight: Bacillus sp. spore powder 1-5%, ethyl acetate 10-20%, amino acid 10-20%, Jinggang 5 to 10% of mycin, 1 to 5% of fenugreek, and 40 to 60% of light rare earth.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于，由以下重量百分比原料组成：芽孢杆菌菌属孢子粉3％、乙酸乙酯15％、氨基酸20％、井冈霉素7％、苦皮藤素5％、轻稀土50％。The microbial pesticide preparation for plant protection growth regulation as claimed in claim 1, characterized in that, it is composed of the following raw materials by weight: 3% of Bacillus sp. spore powder, 15% of ethyl acetate, 20% of amino acids, 7% of oxalate, 5% of bittern, and 50% of light rare earth.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于，由以下重量百分比原料组成：芽孢杆菌菌属孢子粉3％、乙酸乙酯20％、氨基酸18％、井冈霉素7％、苦皮藤素2％、轻稀土50％。The microbial pesticide formulation for plant protection growth regulation according to claim 1, characterized in that, it is composed of the following raw materials by weight percentage: Bacillus sp. spore powder 3%, ethyl acetate 20%, amino acid 18%, Jinggang mold Sulphur 7%, picolin 2%, light rare earth 50%.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于：所述芽孢杆菌菌属孢子粉为短小芽孢杆菌孢子粉或球形芽孢杆菌孢子粉。The microbial pesticide formulation for plant protection growth regulation according to claim 1, wherein the Bacillus sp. spore powder is Bacillus pumilus spore powder or Bacillus sphaericus spore powder.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于：所述芽孢杆菌菌属孢子粉的孢子含量为20亿个/克。The microbial pesticide formulation for plant protection growth regulation according to claim 1, wherein the spore content of the Bacillus spore powder is 2 billion/g.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于：所述氨基酸选自丙氨酸、赖氨酸、甲硫氨酸、缬氨酸、苏氨酸中的任一种或两种以上的混合。The microbial pesticide formulation for plant protection growth regulation according to claim 1, wherein the amino acid is selected from any of alanine, lysine, methionine, valine and threonine One or a combination of two or more.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于：还包含维生素。The microbial pesticide formulation for plant protection growth regulation according to claim 1, characterized in that it further comprises vitamins.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于：所述维生素包括维生素B、维生素C。The microbial pesticide formulation for plant protection growth regulation according to claim 1, wherein the vitamins include vitamin B and vitamin C.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于：还包括矿物质。The microbial pesticide formulation for plant protection growth regulation according to claim 1, characterized in that: it further comprises minerals.
- 如权利要求1所述的用于植物保护生长调节的微生物农药制剂，其特征在于：所述矿物质包括硫酸钙、硫酸镁、氯化钠、氯化钾、氯化铁、硫酸铜。The microbial pesticide formulation for plant protection growth regulation according to claim 1, wherein the minerals include calcium sulfate, magnesium sulfate, sodium chloride, potassium chloride, ferric chloride, and copper sulfate.
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