WO2022105326A1

WO2022105326A1 - Bio-reaction chip structure

Info

Publication number: WO2022105326A1
Application number: PCT/CN2021/112860
Authority: WO
Inventors: 吴旭东; 帅宇; 丁格格
Original assignee: 江苏卓微生物科技有限公司
Priority date: 2020-11-18
Filing date: 2021-08-16
Publication date: 2022-05-27

Abstract

A bio-reaction chip structure, comprising a reaction base (1), an elastic substrate (2) and a pressing plate (3). The pressing plate (3) is fixed on the reaction base (1) by means of a support component, the fixed end of the elastic substrate (2) is fixed to one end of the top surface of the reaction base (1), the elastic substrate (2) is folded in a direction away from the top surface of the reaction base (1), the bottom surface of the elastic substrate (2) abuts against the bottom surface of the pressing plate (3), the bottom surface of the elastic substrate (2) is covered by a first coating (41), and the top surface of the reaction base (1) is covered by a second coating (42).

Description

A bioreactor chip structure

CROSS-REFERENCE TO RELATED APPLICATIONS

This disclosure requires the application number 202011295302.8, titled "Microfluidic Bioreactor Chip and its Using Method", filed with the China Patent Office on November 18, 2020, and the application number filed with the China Patent Office on April 28, 2021 202120905863.9, the priority of the Chinese patent application entitled "A bioreaction chip structure", the entire contents of which are incorporated herein by reference.

technical field

The present disclosure relates to a biological reaction chip structure, belonging to the technical field of biochemistry and molecular biology.

Background technique

At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology. Molecular detection products are mainly used in the detection of various clinical departments such as tumor, infection, genetics, and prenatal screening, as well as physical examination centers, technical service centers, third-party testing institutions, and the rapid microbial testing market.

In the current microfluidic bioreaction chip, when the elastic substrate is automatically downwardly attached to the reaction substrate, since the elastic substrate is not stable enough in the process of falling to the reaction substrate, the elastic substrate may be attached to the reaction substrate. It is not firm, the lamination time is slow, and it is still not convenient and stable enough to use.

public content

The present disclosure provides a biological reaction chip structure, which includes a reaction substrate, an elastic substrate and a pressing plate, the pressing plate is fixed on the reaction substrate through a support assembly, and the fixed end of the elastic substrate is fixed at one end of the top surface of the reaction substrate , the elastic substrate is folded along the direction away from the top surface of the reaction substrate and the bottom surface of the elastic substrate is in contact with the bottom surface of the pressing plate, the bottom surface of the elastic substrate is covered with a first coating, and the reaction The top surface of the base body is covered with a second coating;

The first coating film includes a first coating film attached to the bottom surface of the elastic substrate, and a first connecting film connected to the first coating film by bending;

The second coating film comprises a second coating film attached to the top surface of the reaction substrate, and a second connecting film connected to the second coating film by bending;

The first tie film and the second tie film are fixedly connected.

Optionally, biological reaction functional components are arranged in the reaction matrix (1) and/or the elastic substrate (2).

The present disclosure provides a biological reaction chip structure, the biological reaction chip structure includes a reaction substrate (1), an elastic substrate (2) and a pressing plate (3), the pressing plate (3) is fixed on the reaction substrate (1) through a support assembly ), the fixed end of the elastic substrate (2) is fixed at one end of the top surface of the reaction substrate (1), and the elastic substrate (2) is folded along the direction away from the top surface of the reaction substrate (1) and The bottom surface of the elastic substrate (2) is in contact with the bottom surface of the pressing plate (3), and the bottom surface of the elastic substrate (2) and the top surface of the reaction matrix (1) are connected by an isolation component.

Optionally, the isolation component encloses the biological response functional component.

Optionally, the biological reaction functional component includes a gas hole (7) and a cavity (8).

Optionally, the entire surface of the isolation component seals the top surface of the reaction matrix (1) and the bottom surface of the elastic substrate (2).

Optionally, the isolation assembly includes a first coating film (41) attached to the bottom surface of the elastic substrate (2), and a second coating membrane (41) attached to the top surface of the reaction substrate (1). 42); the first covering film (41) and the second covering film (42) are connected to each other.

Optionally, the first coating film (41) includes a first coating film (411) attached to the bottom surface of the elastic substrate (2), and a first coating film (411) that is bent and connected to the first coating film (411). a connecting membrane (412);

The second coating film (42) comprises a second coating film (421) attached to the top surface of the reaction substrate (1), and a second connecting film (421) bent and connected to the second coating film (421). 422);

The first tie film (412) and the second tie film (422) are fixedly connected.

Optionally, the first joint film (412) and the second joint film (422) are adhered together on their entire surfaces.

Optionally, the first application film (411) and the second application film (421) respectively seal the biological response functional component.

Optionally, the support assembly includes one of a support column, a support frame or a support rod.

Optionally, the support assembly includes a first support column, a second support column, a third support column, and a fourth support column, and the first support column and the second support column are respectively disposed on the reaction substrate near the elastic substrate. At one end of the fixed end, the third support column and the fourth support column are respectively arranged at one end of the reaction substrate away from the fixed end of the elastic substrate.

Optionally, the connection relationship between the first support column (51), the second support column (52), the third support column (53) and the fourth support column (54) and the reaction matrix 1 is as follows: One: One-piece injection molding structure, adhesive connection, riveting, threaded connection, pin connection, plug connection or plastic hot melt welding.

Optionally, one side of the pressing plate is provided with a first connecting plate and a third connecting plate, the other side of the pressing plate is provided with a second connecting plate and a fourth connecting plate, the first connecting plate and the first connecting plate are The support column is fixedly connected, the second connection plate is fixedly connected to the second support column, the third connection plate is fixedly connected to the third support column, and the fourth connection plate is fixedly connected to the fourth support column.

Optionally, between the first connection plate (61) and the first support column (51), between the second connection plate (62) and the second support column (52), the third connection plate Between (63) and the third support column (53), the fixed connection between the fourth connecting plate (64) and the fourth support column (54) is adhesive connection, riveting, screw connection, pin connection, One of plug-in or plastic hot melt welding.

Optionally, the pressing plate is a rigid plate.

Optionally, the pressing plate is a highly elastic flexible sheet.

Optionally, the elastic substrate (2) is a sheet with resilience.

Description of drawings

In order to illustrate the technical solutions of the embodiments of the present disclosure more clearly, the accompanying drawings required in the embodiments will be briefly introduced below. It should be understood that the following drawings merely represent the embodiments of the present disclosure by way of example, and the size ratios in the figures are It does not directly correspond to the true scale of the embodiments, and the following drawings illustrate only certain embodiments of the present disclosure and should not be considered as limiting the scope.

1 is a front view of a structure of a biological reaction chip according to an embodiment of the disclosure;

2 is a top view of a structure of a biological reaction chip according to an embodiment of the disclosure;

3 is a schematic diagram of the connection of the first coating film and the second coating film according to an embodiment of the disclosure;

4 is a schematic structural diagram of a first coating film according to an embodiment of the disclosure;

5 is a schematic structural diagram of a second coating film according to an embodiment of the disclosure;

FIG. 6 is a schematic diagram of the connection between the reactive substrate and the elastic substrate according to an embodiment of the disclosure.

FIG. 7 is a schematic diagram of the connection between the reactive substrate and the elastic substrate according to an embodiment of the disclosure.

8 is a schematic diagram of the structure of the air hole and the cavity and the flow of gas between the air hole and the cavity according to an embodiment of the disclosure, wherein the arrows indicate the flow direction of the gas.

9 is a schematic diagram of the structure of the air hole and the cavity and the flow of gas between the air hole and the cavity according to an embodiment of the disclosure, wherein the arrows indicate the flow direction of the gas.

10 is a front view of a structure of a biological reaction chip according to an embodiment of the disclosure;

11 is a top view of a structure of a biological reaction chip according to an embodiment of the disclosure;

FIG. 12 is a schematic diagram of the connection between the reactive substrate and the elastic substrate according to an embodiment of the disclosure.

13 is a top view of a structure of a biological reaction chip according to an embodiment of the disclosure.

Reference numerals: reactive substrate 1, elastic substrate 2, pressing plate 3, first coating 41, first applying film 411, first connecting film 412, second coating 42, second applying film 421, second The connecting film 422, the first supporting column 51, the second supporting column 52, the third supporting column 53, the fourth supporting column 54, the first connecting plate 61, the second connecting plate 62, the third connecting plate 63, the fourth connecting plate 64. Air hole 7, air hole cavity 71, ventilation hole 72, through hole 73, cavity 8, liquid storage cavity 81, reaction cavity 82, sample addition cavity 83, buffer cavity 84, waste liquid cavity 85, microfluidic channel 9, support rack 10.

Detailed ways

In order to make the content of the present disclosure easier to understand clearly, the present disclosure will be described in further detail below according to the embodiments and in conjunction with the accompanying drawings.

I. Bioreactor chip structure

An embodiment of the present disclosure discloses a biological reaction chip structure including a reaction substrate 1, an elastic substrate 2 and a pressure plate 3. The pressure plate 3 is fixed on the reaction substrate 1 through a support assembly, and the fixed end of the elastic substrate 2 is fixed At one end of the top surface of the reaction substrate 1 , the elastic substrate 2 is folded in a direction away from the top surface of the reaction substrate 1 and the bottom surface of the elastic substrate 2 is in contact with the bottom surface of the pressing plate 3 . The bottom surface of 2 and the top surface of the reaction substrate 1 are connected by isolation components.

In some embodiments, biological reaction functional components are provided in the reaction matrix 1 and/or the elastic substrate 2 .

In some exemplary embodiments, the biological reaction functional components include, but are not limited to, pores 7 , cavities 8 and microfluidic channels 9 .

In some embodiments, the chamber 8 includes, but is not limited to, a liquid storage chamber 81 , a reaction chamber 82 , a sample addition chamber 83 , a buffer chamber 84 and a waste chamber 85 .

In some embodiments, the air holes 7 include, but are not limited to, air hole cavities 71 , vent holes 72 or through holes 73 .

In some exemplary embodiments, the end face of the reaction substrate 1 facing the elastic substrate 2 is provided with an air hole cavity 71 , a liquid storage chamber 81 , a reaction chamber 82 , a buffer chamber 84 and a waste liquid chamber 85 , and the elastic substrate 2 faces the reaction substrate. The end face of 1 is provided with microfluidic channels 9 and ventilation holes 72 .

In some exemplary embodiments, the end face of the reaction substrate 1 facing the elastic substrate 2 is provided with a pore cavity 71 , a liquid storage cavity 81 , a reaction cavity 82 , a sample loading cavity 83 , a buffer cavity 84 and a waste liquid cavity 85 . The end face of the sheet 2 facing the reaction matrix 1 is provided with a microfluidic channel 9 and a vent hole 72 .

In some exemplary embodiments, the air hole cavity 71 is configured with a closed lower surface and an open upper surface; the ventilation hole 72 is a ventilation hole with both upper and lower surfaces open. In some embodiments, at least one liquid storage cavity 81 corresponds to at least one air hole cavity 71 . In some embodiments, at least one vent hole 72 corresponds to at least one vent cavity 71 . In some embodiments, at least one vent hole 72 is located at one end of the microfluidic channel 9 and communicates with the corresponding at least one air hole cavity 71 through the microfluidic channel 9 .

In some embodiments, the reaction substrate 1 is provided with at least one liquid storage chamber 81, at least one air hole chamber 71, at least one sample adding chamber 83, and a reaction chamber 82;

The end face of the elastic substrate facing the reaction substrate is provided with a plurality of microfluidic channels 9;

When the elastic substrate is attached to the reaction substrate, some of the microfluidic channels 9 of the plurality of microfluidic channels are configured to communicate with the liquid storage chamber 81 and the air cavity 71, and some of the microfluidic channels 9 are configured to communicate with the liquid storage chamber 81 and the reaction chamber. The cavity 82 , part of the microfluidic channel 9 is configured to communicate with the sample adding cavity 83 and the air hole cavity 71 , and part of the microfluidic channel 9 is configured to communicate with the sample adding cavity 83 and the reaction chamber 82 . In some embodiments, at least one buffer chamber 84 is further provided on the reaction substrate 1 , and part of the microfluidic channel 9 is configured to communicate with the reaction chamber 82 and the buffer chamber 84 .

In some exemplary embodiments, adjacent liquid storage cavities 81 are provided as a structure that communicates inside the reaction matrix 1 , such as through a U-shaped channel in the reaction matrix 1 . In some exemplary embodiments, the liquid storage cavity 81 is configured to correspond to one end of the microfluidic channel 9. When the reaction matrix 1 and the elastic substrate 2 are attached to each other, part of the liquid storage cavity 81 and the reaction cavity 82 pass through the microfluidic channel 9 is connected, and part of the liquid storage cavity 81 is communicated with the air hole cavity 71 through the microfluidic channel 9 .

In some embodiments, the sample adding cavity 83 and the buffer cavity 84 are closed on the lower surface and open on the upper surface. In some embodiments, the buffer cavity 84 is configured to correspond to one end of the microfluidic channel 9 . When the reaction matrix 1 and the elastic substrate 2 are attached, the buffer cavity 84 communicates with the reaction cavity 82 through the microfluidic channel 9 .

In some embodiments, the buffer chamber 84 and the sample loading chamber 83 are configured as a structure that communicates inside the reaction matrix 1 , such as through a U-shaped channel together inside the reaction matrix 1 .

In some embodiments, the sample adding cavity 83 is configured to correspond to one end of the microfluidic channel 9 . When the reaction matrix 1 and the elastic substrate 2 are attached, the sample adding cavity 83 communicates with the vent hole 72 through the microfluidic channel 9 .

When the reaction substrate 1 and the elastic substrate 2 are bonded together, the buffer chamber 84 and the reaction chamber 82 communicate with each other through the microfluidic channel 9, and the buffer chamber 84 and the sample adding chamber 83 communicate with each other. The ventilation holes 72 on the elastic elastic substrate 2 are connected to the external air source for driving.

In some exemplary embodiments, when the reaction matrix 1 and the elastic substrate 2 are attached, the vent hole 72 can be connected with a fluid driving device to drive the fluid, and some of the microfluidic channels in the plurality of microfluidic channels 9 9 is configured to communicate with the liquid storage chamber 81 and the stomata chamber 71, and part of the microfluidic channel 9 is configured to communicate with the liquid storage chamber 81, the reaction chamber 82 and the waste liquid chamber 85, thereby forming a complete closed environment for fluid driving and biological reactions .

In some embodiments, the pressing plate 3 is provided with through holes 73 . In some embodiments, the through hole 73 is a vent hole opened up and down. In some embodiments, the through holes 73 are arranged so that when the reaction matrix 1 and the elastic substrate 2 are attached, the through holes 73 are positioned vertically corresponding to the ventilation holes 72 .

In some embodiments, the shape of the through hole 73 provided on the pressing plate 3 may be, for example, including but not limited to round, square, diamond or window through holes, as long as it satisfies, when the reaction substrate 1 and the elastic substrate 2 are bonded together , just above the pressing plate and in the vertical direction of the elastic substrate 2 , part or all of the ventilation holes 72 can be exposed through the through holes 73 . When the reaction substrate 1 and the elastic substrate 2 are bonded together, an external air source can be connected to the vent hole 72 through the through hole 73 to drive the flow of the liquid.

In some embodiments, the isolation assembly encloses the biological response functional assembly.

In some embodiments, the isolation component completely seals the top surface of the reaction matrix 1 and the bottom surface of the elastic substrate 2 .

In some embodiments, the isolation component includes a first coating 41 attached to the bottom surface of the elastic substrate 2, and a second coating 42 attached to the top surface of the reactive substrate 1; the first coating The first coating 41 and the second coating 42 are connected to each other.

In some exemplary embodiments, the cavity 8 is a semi-open structure and is covered by the second cover film 42 .

In some embodiments, the first coating film 41 includes a first coating film 411 attached to the bottom surface of the elastic substrate 2, and a first connecting film 412 connected to the first coating film 411 by bending;

The second coating film 42 includes a second coating film 421 attached to the top surface of the reaction substrate 1, and a second connecting film 422 connected to the second coating film 421 by bending;

The first connecting film 412 and the second connecting film 422 are fixedly connected.

An embodiment of the present disclosure provides a biological reaction chip structure, which includes a reaction substrate 1 , an elastic substrate 2 and a pressing plate 3 , the pressing plate 3 is fixed on the reaction substrate 1 by a support assembly, and the fixed end of the elastic substrate 2 is It is fixed at one end of the top surface of the reaction substrate 1, the elastic substrate 2 is folded along the direction away from the top surface of the reaction substrate 1, and the bottom surface of the elastic substrate 2 is in contact with the bottom surface of the pressing plate 3. The bottom surface of the sheet 2 is covered with a first coating 41, and the top surface of the reaction substrate 1 is covered with a second coating 42;

The first coating film 41 includes a first coating film 411 attached to the bottom surface of the elastic substrate 2, and a first connecting film 412 connected to the first coating film 411 by bending;

In some embodiments, the first application film 411 and the second application film 421 respectively seal the biological response functional components.

II. Support components

In some embodiments, the support assembly includes, but is not limited to, a support column, a support frame 10, or a support rod. It should be noted that, as long as the support assembly is sufficient to fix the pressure plate 3 above the elastic substrate 2, the elastic substrate 2 can be fixed by the gap before the chip is used and/or after the connecting film is torn off, the elastic substrate 2 can pass through The slide is attached to the reactive matrix 1.

In some embodiments, the support assembly includes a first support column 51, a second support column 52, a third support column 53 and a fourth support column 54, and the first support column 51 and the second support column 52 are respectively provided At one end of the reaction substrate 1 close to the fixed end of the elastic substrate 2 , the third support column 53 and the fourth support column 54 are respectively disposed at one end of the reaction substrate 1 away from the fixed end of the elastic substrate 2 . This embodiment is non-limiting, and in other embodiments, the support assembly may also include two, three, five or more support posts.

In some embodiments, the support assembly is a support frame 10 , and the support frame 10 is fixed above the reaction matrix 1 . In some embodiments, the fixing method of the support frame 10 and the reaction matrix 1 includes, but is not limited to, bonding, snap-fit, chute or integral molding. In some embodiments, one end of the support frame 10 is disposed at one end of the reaction substrate 1 close to the fixed end of the elastic substrate 2 , and the other end of the support frame is disposed at one end of the reaction substrate 1 away from the fixed end of the elastic substrate 2 . In some embodiments, the support frame 10 is configured to open on the side close to the fixed end of the reaction substrate 1 and the elastic substrate 2 , and open on the side of the reaction substrate 1 away from the fixed end of the elastic substrate 2 . It is believed that, without being bound by theory, the openings on both sides of the support frame are provided to realize the fixing of the elastic substrate 2, and the elastic substrate 2 can be attached to the reaction substrate 1 by sliding after the bonding film is torn off. In some embodiments, the pressing plate 3 is fixed on the lower surface of the top end of the support frame 10 . In some embodiments, the fixing methods of the pressing plate 3 and the support frame 10 include, but are not limited to, bonding and crimping.

In some embodiments, the connection relationship between the first support column 51 , the second support column 52 , the third support column 53 and the fourth support column 54 and the reaction matrix 1 is one of the following: integral injection molding Structural, glued, riveted, threaded, pinned, plugged or plastic hot melt welding.

In some embodiments, a first connecting plate 61 and a third connecting plate 63 are disposed on one side of the pressing plate 3, and a second connecting plate 62 and a fourth connecting plate 64 are disposed on the other side of the pressing plate 3, so The first connecting plate 61 and the first supporting column 51 are fixedly connected, the second connecting plate 62 and the second supporting column 52 are fixedly connected, the third connecting plate 63 and the third supporting column 53 are fixedly connected, and the The four connecting plates 64 are fixedly connected to the fourth supporting column 54 .

In some embodiments, between the first connection plate 61 and the first support column 51 , between the second connection plate 62 and the second support column 52 , and between the third connection plate 63 and the third support column 53, the fixed connection between the fourth connection plate 64 and the fourth support column 54 is one of adhesive connection, riveting, screw connection, pin connection, plug connection or plastic hot melt welding.

III. SELECTION OF PLATE AND ELASTIC SUBSTRATE

In some embodiments, the pressing plate 3 is a rigid plate.

In some embodiments, the pressing plate 3 is a highly elastic flexible sheet.

In some embodiments, the elastic substrate 2 is a sheet with resilience.

As used herein, the term "resilience" refers to the ability of an object to rapidly return to its original shape after the external force causing the object to deform is removed.

The embodiments of the present disclosure overcome the deficiencies of the prior art, and provide a biological reaction chip structure, which can make the bonding between the elastic substrate 2 and the reaction matrix 1 more ideal.

By adopting the above technical solution, the present disclosure separates the coating film on the reaction substrate 1 and the elastic substrate 2 by pulling the first connecting film 412 and the second connecting film 422 that are pasted together, and the pulling process is matched with the pressing of the pressing plate 3 at the same time. The force makes the bonding process of the elastic substrate 2 and the reaction matrix 1 faster, smoother and more stable, and the bonding effect is more ideal than natural bonding.

As shown in Figures 1 to 6, a biological reaction chip structure includes a reaction matrix 1, an elastic substrate 2 and a pressure plate 3. The reaction matrix 1 and the elastic substrate 2 are provided with a liquid storage cavity for biological reaction, a reaction In the cavity, flow channel and air hole, etc., the top surface of the reaction substrate 1 is fixed with a support component, the bottom surface of the pressure plate 3 is fixed on the support component, and the fixed end of the elastic substrate 2 is fixed on one end of the top surface of the reaction substrate 1. The sheet 2 is folded in a direction away from the top surface of the reaction substrate 1 and the bottom surface of the elastic substrate 2 is in contact with the bottom surface of the pressing plate 3 .

The elastic substrate 2 can be but is not limited to high molecular polymers such as PDMS, Flexdym, epoxy resin, polyurethane, etc., that is, as long as the selected material of the elastic substrate 2 can have a certain resilience, it can meet the requirements in the process of tearing off the film. Among them, the elastic substrate can rebound, so that the elastic substrate 2 and the reaction matrix 1 are attached, which can meet the usage requirements of this embodiment. The pressing plate 3 may be a rigid plate, or may be, but not limited to, a high-elasticity flexible sheet such as latex coating, TPU coating, silicone coating, and PVC coating. In the process of applying the material of the pressing plate 3, during the process of pulling 422 and 412 to complete the bonding, the first film 41 can slide on the lower surface of the pressing plate 3 with a certain frictional force, but will not stick. The pressing plate 3 has sufficient pressing force on the elastic substrate 2 . The elastic substrate 2 is reversely pressed by the pressing plate 3, and the pressing plate 3 will exert a downward pressing force on the folded elastic substrate 2, and then use the resilience of the elastic substrate 2 itself in the process of tearing off the film. , the effect of bonding the elastic substrate 2 and the reaction matrix 1 is better.

As shown in FIGS. 1 to 5 , the bottom surface of the elastic substrate 2 is covered with a first coating 41 , and the top surface of the reaction substrate 1 is covered with a second coating 42 .

Among them, as shown in FIGS. 3 to 5 , the first coating film 41 includes a first coating film 411 attached to the bottom surface of the elastic substrate 2 , and a first connecting film 412 that is bent and connected to the first coating film 411 . The second coating film 42 includes a second coating film 421 attached to the top surface of the reaction substrate 1, and a second bonding film 422 connected to the second bonding film 421 by bending; the first bonding film 412 and the second bonding film 422 The joint films 422 are adhered together on the entire surface, and the first and

second application films

411 and 421 respectively seal the bioreaction functional components, such as pores and cavities, inside the reaction substrate 1 and the elastic substrate 2 .

When in use, just pull the first connecting film 412 and the second connecting film 422 pasted together outward to drive the first sticking film 411 to separate from the bottom surface of the elastic substrate 2, and also drive the second sticking film 421 to be separated from the bottom surface of the elastic substrate 2. The top surface of the reaction substrate 1 is separated, and during the process of separating the first application film 411 from the elastic substrate 2, the first application film 411 will drive the bottom surface of the elastic substrate 2 to adhere to the top surface of the reaction substrate 1. With the force of the pressing plate 3 pressing down on the elastic substrate 2, the elastic substrate 2 will fall and adhere to the reaction matrix 1 more quickly and stably.

When the elastic substrate 2 with opposite surface charge and the reaction substrate 1 are attached together, electrostatic adsorption is added, and the attachment effect is better.

As shown in FIG. 6 , the pressure plate 3 is connected to the reaction substrate 1 through a support assembly. In this embodiment, a preferred solution of the support assembly is provided. The support assembly mainly plays the role of fixing the pressure plate 3 above the reaction substrate 1 and is not limited to The structure of the support assembly in this embodiment. The support assembly in this embodiment includes a first support column 51 , a second support column 52 , a third support column 53 and a fourth support column 54 . The first support column 51 and the second support column 52 are respectively disposed on the reaction substrate 1 . One end close to the fixed end of the elastic substrate 2, the third support column 53 and the fourth support column 54 are respectively arranged at one end of the reaction substrate 1 away from the fixed end of the elastic substrate 2, each support column of the support assembly in this embodiment and the reaction substrate 1 One-piece injection molding structure.

As shown in FIG. 2 , one side of the pressing plate 3 is provided with a first connecting plate 61 and a third connecting plate 63 , the other side of the pressing plate 3 is provided with a second connecting plate 62 and a fourth connecting plate 64 , and the first connecting plate 61 It is fixedly connected with the first support column 51, the second connecting plate 62 is fixedly connected with the second support column 52, the third connecting plate 63 is fixedly connected with the third support column 53, and the fourth connecting plate 64 is fixedly connected with the fourth support column 54. . The connection method between each connecting plate on both sides of the pressing plate 3 and the corresponding support column can be by gluing or plastic hot melt welding.

As shown in FIG. 7 , on the basis of FIG. 6 , the end face of the reaction substrate 1 facing the elastic substrate 2 is provided with an air hole cavity 71 , a liquid storage cavity 81 , a reaction cavity 82 , a sample addition cavity 83 , a buffer cavity 84 and a waste liquid cavity 85. The end face of the elastic substrate 2 facing the reaction substrate 1 is provided with a microfluidic channel 9 and a ventilation hole 72.

The air hole cavity 71 , the liquid storage cavity 81 , the reaction cavity 82 , the sample addition cavity 83 , the buffer cavity 84 and the waste liquid cavity 85 can all be configured so that the lower surface is closed and the upper surface is open; stomata. Each liquid storage cavity 81 corresponds to each air hole cavity 71 . Each air hole 72 corresponds to each air hole cavity 71 .

As shown in FIGS. 7 and 8 , the adjacent liquid storage cavities 81 are arranged to communicate with each other through a U-shaped channel inside the reaction matrix 1 , and the liquid storage cavity 81 is configured to correspond to one end of the microfluidic channel 9 . When the substrate 1 and the elastic substrate 2 are attached, between the adjacent liquid storage chambers 81, one of the liquid storage chambers 81 is communicated with the reaction chamber 82 through the microfluidic channel 9, and the other adjacent liquid storage chamber 81 is connected to the air hole. The chambers 71 are communicated through the microfluidic channel 9 . At the same time, the air hole cavity 71 is configured to correspond to one end of the microfluidic channel 9 . When the reaction matrix 1 and the elastic substrate 2 are attached, the air hole cavity 71 and the corresponding ventilation hole 72 are communicated through the microfluidic channel 9 . When the reaction substrate 1 and the elastic substrate 2 are attached together, the vent hole 72 can be connected with the fluid driving device to drive the fluid, and some of the microfluidic channels 9 in the plurality of the microfluidic channels 9 are configured to communicate the liquid storage chamber 81 with the The stomata cavity 71 and part of the microfluidic channel 9 are configured to communicate with the liquid storage cavity 81 , the reaction cavity 82 and the waste liquid cavity 85 , thereby forming a complete closed environment for fluid driving and biological reactions.

As shown in FIGS. 7 and 9 , on the basis of the structure in FIG. 8 , the buffer chamber 84 and the sample adding chamber 83 can be configured to be connected through a U-shaped channel inside the reaction matrix 1 , and the sample adding chamber 83 can be configured to correspond to the micro-channel. At one end of the flow channel 9 , when the reaction matrix 1 and the elastic substrate 2 are attached, the sample loading chamber 83 is communicated with the vent hole 72 through the microfluidic channel 9 . At the same time, the buffer cavity 84 is configured to correspond to one end of the microfluidic channel 9 . When the reaction matrix 1 and the elastic substrate 2 are attached, the buffer cavity 84 and the reaction cavity 82 communicate with each other through the microfluidic channel 9 . When the reaction substrate 1 and the elastic substrate 2 are bonded together, the buffer chamber 84 and the reaction chamber 82 communicate with each other through the microfluidic channel 9, and the buffer chamber 84 and the sample adding chamber 83 communicate with each other. The ventilation holes 72 on the elastic elastic substrate 2 are communicated with each other and connected to the external air source drive, thereby forming a complete closed environment for fluid drive and biological reaction.

On the basis of FIGS. 7-9 , the reaction chamber 82 can also be configured so that the left and right ends of the reaction chamber 82 correspond to a microfluidic channel 9 respectively. When the reaction substrate 1 and the elastic substrate 2 are attached, the left and right ends of the reaction chamber 82 are respectively connected to The corresponding liquid storage chamber 81 and the buffer chamber 84 are communicated with each other through the microfluidic channel 9 . As shown in FIGS. 10-12 , the support component is a support frame 10 , the support frame 10 is fixed above the reaction substrate 1 by bonding, and the pressing plate 3 is fixed on the supporting frame in a direction parallel to the pressing plate 3 by bonding 10 The lower surface of the tip. One end of the support frame 10 in the x-axis direction is disposed at one end of the reaction substrate 1 close to the fixed end of the elastic substrate 2 , and the other end of the support frame in the x-axis direction is disposed at one end of the reaction substrate 1 away from the fixed end of the elastic substrate 2 . At the same time, the support frame 10 is arranged to open on the side of the reaction base 1 and the fixed end of the elastic substrate 2 in the x-axis direction, and open on the side of the reaction base 1 away from the fixed end of the elastic substrate 2, and at the same time in the y-axis direction. opening on both sides. The openings on both sides of the support frame in the x-axis direction are to realize the fixation of the elastic substrate 2, and the elastic substrate 2 can be attached to the reaction matrix 1 by sliding after tearing off the connecting film. The opening in the y-axis direction is for the purpose of Save material.

As shown in FIG. 13 , the pressing plate 3 is provided with through holes 73 , such as a plurality of through holes 73 , and the through holes 73 are ventilation holes opened up and down. The through holes 73 are configured such that when the reaction substrate 1 and the elastic substrate 2 are attached, each through hole 73 corresponds to the position of each vent hole 72 , so that each through hole 73 is in fluid communication with each vent hole 72 . When the reaction matrix 1 and the elastic substrate 2 are attached, each through hole 73 and each corresponding vent hole 72 respectively form a flow channel directly above the pressing plate and in the vertical direction of the elastic substrate 2 . When the reaction substrate 1 and the elastic substrate 2 are bonded together, the external air source can be connected to the vent hole 72 through the through hole 73 to drive the liquid flow.

During production and installation, the pressing plate 3 can be inclined toward the pulling direction of the first connecting film 412 and the second connecting film 422, which is more favorable for the elastic substrate 2 to be attached to the reaction substrate 1 during the rebound process.

The above embodiments further describe in detail the technical problems, technical solutions and beneficial effects solved by the present disclosure. It should be understood that the above are only examples of the present disclosure, and are not intended to limit the present disclosure. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present disclosure shall be included within the protection scope of the present disclosure.

Industrial Applicability

The present disclosure provides a biological reaction chip structure. The reaction substrate and the cover film on the elastic substrate are separated by pulling the first connecting film and the second connecting film that are pasted together. During the pulling process, the pressing force of the pressing plate is simultaneously matched, so that The bonding process between the elastic substrate and the reactive matrix is faster, smoother and more stable, and the bonding effect is more ideal than natural bonding, which has a wide range of industrial application value.

Claims

A biological reaction chip structure, characterized in that: the biological reaction chip structure comprises a reaction matrix (1), an elastic substrate (2) and a pressure plate (3), and the pressure plate (3) is fixed on the reaction matrix (3) by a support assembly. 1), the fixed end of the elastic substrate (2) is fixed at one end of the top surface of the reaction substrate (1), and the elastic substrate (2) is folded along the direction away from the top surface of the reaction substrate (1) And the bottom surface of the elastic substrate (2) is in contact with the bottom surface of the pressing plate (3), the bottom surface of the elastic substrate (2) is covered with a first coating film (41), and the reaction matrix (1) has a bottom surface. The top surface is covered with a second film (42);

The first coating film (41) includes a first coating film (411) attached to the bottom surface of the elastic substrate (2), and a first connecting film (411) that is bent and connected to the first coating film (411). 412);

The second coating film (42) comprises a second coating film (421) attached to the top surface of the reaction substrate (1), and a second connecting film (421) bent and connected to the second coating film (421). 422);

The first tie film (412) and the second tie film (422) are fixedly connected.
The structure of a biological reaction chip according to claim 1, wherein a biological reaction functional component is arranged in the reaction matrix (1) and/or the elastic substrate (2).
A biological reaction chip structure according to claim 2, characterized in that: the biological reaction chip structure comprises a reaction matrix (1), an elastic substrate (2) and a pressure plate (3), the pressure plate (3) passing through The support assembly is fixed on the reaction base (1), the fixed end of the elastic base sheet (2) is fixed at one end of the top surface of the reaction base (1), and the elastic base sheet (2) is along a distance away from the reaction base (1). ) is folded in the direction of the top surface and the bottom surface of the elastic substrate (2) is in contact with the bottom surface of the pressing plate (3), and the bottom surface of the elastic substrate (2) and the top surface of the reaction substrate (1) are in contact with each other. connected by isolation components.
The structure of a biological reaction chip according to claim 3, wherein the isolation component seals the biological reaction functional component.
The structure of a biological reaction chip according to claim 3 or 4, characterized in that: the isolation component seals the entire surface of the top surface of the reaction substrate (1) and the bottom surface of the elastic substrate (2).
A bioreaction chip structure according to any one of claims 3-5, characterized in that: the isolation component comprises a first covering film (41) attached to the bottom surface of the elastic substrate (2), A second coating film (42) attached to the top surface of the reaction substrate (1); the first coating film (41) and the second coating film (42) are connected to each other.
A kind of biological reaction chip structure according to claim 6, is characterized in that:

The first coating film (41) includes a first coating film (411) attached to the bottom surface of the elastic substrate (2), and a first connecting film (411) that is bent and connected to the first coating film (411). 412);

The second coating film (42) comprises a second coating film (421) attached to the top surface of the reaction substrate (1), and a second connecting film (421) bent and connected to the second coating film (421). 422);

The first tie film (412) and the second tie film (422) are fixedly connected.
A bioreaction chip structure according to claim 2 or 7, characterized in that: the first application film (411) and the second application film (421) respectively seal the bioreaction functional components.
A bioreaction chip structure according to any one of claims 1-8, characterized in that: the support assembly comprises one of a support column, a support frame (10) or a support rod.
A bioreactor chip structure according to any one of claims 1-8, wherein the support assembly comprises a first support column (51), a second support column (52), a third support column ( 53) and the fourth support column (54), the first support column (51) and the second support column (52) are respectively arranged at one end of the reaction substrate (1) close to the fixed end of the elastic substrate (2), the The third support column (53) and the fourth support column (54) are respectively arranged at one end of the reaction substrate (1) away from the fixed end of the elastic substrate (2).
A bioreactor chip structure according to claim 10, characterized in that: the first support column (51), the second support column (52), the third support column (53) and the fourth support column (54) ) and the reaction matrix (1) are respectively connected to one of the following: integral injection molding structure, adhesive connection, riveting, screw connection, pin connection, plug connection or plastic hot melt welding.
A biological reaction chip structure according to claim 10 or 11, characterized in that: a first connecting plate (61) and a third connecting plate (63) are provided on one side of the pressing plate (3), and the pressing plate The other side of (3) is provided with a second connecting plate (62) and a fourth connecting plate (64), the first connecting plate (61) and the first supporting column (51) are fixedly connected, the second connecting The plate (62) is fixedly connected to the second support column (52), the third connection plate (63) is fixedly connected to the third support column (53), and the fourth connection plate (64) is connected to the fourth support column ( 54) Fixed connection.
A bioreactor chip structure according to claim 12, characterized in that: between the first connecting plate (61) and the first support column (51), the second connecting plate (62) and the second connecting plate (62) Fixed connection between the support columns (52), between the third connection plate (63) and the third support column (53), and between the fourth connection plate (64) and the fourth support column (54) The method is one of glue connection, riveting, screw connection, pin connection, plug connection or plastic hot melt welding.
A biological reaction chip structure according to any one of claims 1-13, characterized in that: the reaction substrate (1) is provided with at least one liquid storage chamber (81), at least one air hole chamber (71), at least one a sample loading chamber (83), and a reaction chamber (82);

The end face of the elastic substrate facing the reaction substrate is provided with a plurality of microfluidic channels (9);

When the elastic substrate is attached to the reaction substrate, some of the microfluidic channels (9) in the plurality of microfluidic channels are configured to communicate with the liquid storage chamber (81) and the air cavity (71), and some of the microfluidic channels (9) are configured The liquid storage chamber (81) and the reaction chamber (82) are connected to each other, and part of the microfluidic channel (9) is configured to communicate with the sample injection chamber (83) and the air hole chamber (71), and part of the microfluidic channel (9) is configured to communicate with the pumping chamber. The sample chamber (83) and the reaction chamber (82).
The structure of a biological reaction chip according to claim 14, characterized in that: the pressing plate (3) is provided with a through hole (73), and the through hole (73) is provided with upper and lower openings. When the elastic substrate 2 is attached, the through hole (73) is at a position vertically corresponding to the ventilation hole (72).
A biological reaction chip structure according to any one of claims 1-15, characterized in that: the pressing plate (3) is a rigid plate.
A biological reaction chip structure according to any one of claims 1-16, characterized in that: the pressing plate (3) is a highly elastic flexible sheet.
A bioreaction chip structure according to any one of claims 1-17, characterized in that: the elastic substrate (2) is a thin sheet with resilience.