JP2020024202A - Analysis tool and cleaning method - Google Patents

Abstract

To provide an analysis tool capable of increasing a recovery rate of a target substance.SOLUTION: An analysis tool 1 includes a flow path 2 for feeding fluid, and a recovery part 3 that is provided in the middle of a flow path 2a and is filled with a solid-phase carrier 6 for recovering and cleaning a target substance by a solid-phase extraction method, and the flow path 2 includes the first flow path 2a ranging with an upstream side of the recovery part 3, and a second flow path 2b ranging with a downstream side of the recovery part 3. A ratio φ/W1 and a ratio φ/W2 are within ranges of 1.5 or larger and 6 or smaller, respectively when a planar shape of the recovery part 3 is nearly elliptical, and a width of the first flow path 2a is set to W1, a width of the second flow path 2b is set to W2, and a diameter of the recovery part 3 is set to φ when the recovery part 3 is circular, or (a long diameter + a short diameter)/2 is set to φ when the recovery part 3 is elliptical in a plan view.SELECTED DRAWING: Figure 1

Description

  The present invention relates to an analysis tool and a method for cleaning a target substance using the analysis tool.

  2. Description of the Related Art Conventionally, various analytical tools provided with a microchannel through which a fluid is sent have been proposed. This type of analysis tool is used for analysis of biological substances such as nucleic acids and enzymes, analysis of inorganic ions, and the like. A target substance such as a nucleic acid used for such biochemical and chemical analysis may be separated and collected by being carried on a carrier.

  Specifically, in the solid-phase extraction method, a target substance such as a nucleic acid is carried in a collecting section filled with a solid-phase carrier. The target substance carried on the solid phase carrier in the recovery section is washed with a washing liquid. Then, the target substance is separated and recovered by the recovery liquid. In Patent Literature 1, a fluid is sent from above and below to a solid phase extraction column as such a recovery unit, and washing and recovery are performed.

JP 2011-158450 A

  However, in the analysis tool using the solid-phase extraction method as in Patent Literature 1, a liquid residue may be left in the recovery part. In addition, drying of a target substance such as a nucleic acid may be insufficient. Therefore, it has been difficult to sufficiently increase the recovery rate of the target substance.

  An object of the present invention is to provide an analysis tool and a cleaning method using the analysis tool, which can increase the recovery rate of a target substance.

  The analysis tool according to the present invention is provided with a flow path through which a fluid is sent, and provided in the middle of the flow path, and filled with a solid phase carrier for collecting and washing a target substance by a solid phase extraction method. A collecting section, wherein the flow path has a first flow path connected to an upstream side of the collecting section, and a second flow path connected to a downstream side of the collecting section. The planar shape of the collecting section is substantially circular, and the width of the first flow path is W1, the width of the second flow path is W2, and the shape of the collecting section is circular in plan view. When the diameter of the collecting part is or the shape of the collecting part is elliptical, when (major axis + minor axis) / 2 is φ, the ratio φ / W1 and the ratio φ / W2 are: They are in the range of 1.5 or more and 6 or less, respectively.

  In a specific aspect of the analysis tool according to the present invention, in a plan view, the intersection of an extension line along the direction in which the first flow path extends and an extension line in the direction in which the second flow path extends, It is located so as to overlap the center of gravity of the collection unit.

  In another specific aspect of the analysis tool according to the present invention, a substrate having a concave portion, and a cover member provided on the substrate and closing the concave portion of the substrate, comprising: The recovery part is configured by closing the recess by the cover member.

  In still another specific aspect of the analysis tool according to the present invention, the first flow path, the second flow path, and the collection unit are arranged so as to face the cover member.

  In still another specific aspect of the analysis tool according to the present invention, the first flow path, the second flow path, and the collection unit have the same depth.

  In still another specific aspect of the analysis tool according to the present invention, the cover member is formed of a resin film. It is preferable that the thickness of the resin film is 200 μm or less.

  In still another specific aspect of the analysis tool according to the present invention, the target substance is a nucleic acid.

  In still another specific aspect of the analytical device according to the present invention, the solid support is a glass fiber or a silica monolith.

  The cleaning method of the present invention is a method for cleaning the target substance using the analysis tool configured according to the present invention, wherein a liquid containing the target substance is sent from the first flow path to the recovery unit, Supporting the target substance on the solid phase carrier, sending a cleaning liquid from the first flow path to the collection unit, and washing the target substance supported on the solid phase carrier; Drying the target substance by heating the part.

  In a specific aspect of the cleaning method of the present invention, the analysis tool includes a substrate having a concave portion, and a cover member provided on the substrate and closing the concave portion of the substrate, The recovery unit is configured by closing the concave portion of the substrate by the cover member, and the heating of the recovery unit is performed from the cover member side.

  ADVANTAGE OF THE INVENTION According to this invention, the analysis tool which can raise the recovery rate of a target substance, and the washing | cleaning method using the said analysis tool can be provided.

FIG. 2 is a schematic plan view illustrating a main part of the analysis tool according to the first embodiment of the present invention. FIG. 2 is a schematic cross-sectional view of a portion along line AA and line BB in FIG. 1. It is a schematic plan view for explaining the relation between the intersection of the extension line along the direction in which the first flow path extends and the extension line in the direction in which the second flow path extends, and the center of gravity of the collection unit. FIG. 7 is a schematic plan view illustrating a main part of an analysis tool according to a second embodiment of the present invention. FIG. 5 is a schematic cross-sectional view of a portion along a line CC in FIG. 4.

  Hereinafter, the present invention will be clarified by describing specific embodiments of the present invention with reference to the drawings.

[Analysis tools]
(First embodiment)
FIG. 1 is a schematic plan view showing a main part of the analysis tool according to the first embodiment of the present invention. FIG. 2 is a schematic cross-sectional view of a portion along line AA and line BB in FIG. In addition, the left part of FIG. 2 shows a part along the line AA of FIG. 2 shows a portion along the line BB in FIG. In FIG. 1, the inside of the analysis tool 1 is indicated by a broken line.

  As shown in FIG. 1, the analysis tool 1 is a microchip provided with a flow path 2 through which a fluid is sent. A recovery unit 3 is provided in the middle of the flow path 2. The recovery section 3 is filled with a solid phase carrier 6 for recovering and washing the target substance by a solid phase extraction method. The target substance includes, for example, nucleic acids, pesticides, and the like.

  Although not particularly limited, the analysis tool 1 has a plate-shaped substrate 4 and a cover member 5 in the present embodiment, as shown in FIG. The substrate 4 has a first main surface 4a and a second main surface 4b facing each other. On the first main surface 4a side of the substrate 4, a concave portion 4c is provided. The recess 4c is provided so as to open to the first main surface 4a side.

  The material forming the substrate 4 is not particularly limited, and for example, a synthetic resin, rubber, metal, or the like can be used. The synthetic resin is not particularly limited, but is preferably a thermoplastic resin. Among them, as the thermoplastic resin, for example, a cycloolefin polymer, a cycloolefin copolymer, polycarbonate, polymethyl methacrylate, or polypropylene can be used. One of these may be used alone, or a plurality of them may be used in combination.

  The substrate 4 is preferably made of a molded body of the thermoplastic resin. The molding method is not particularly limited, and a known molding method can be used. Examples of the molding method include injection molding, injection compression molding, gas-assisted injection molding, extrusion molding, multilayer extrusion molding, rotational molding, hot press molding, blow molding, and foam molding. Of these, injection molding is preferred.

  The substrate 4 may be formed by laminating a plurality of synthetic resin sheets. The substrate 4 may include a base sheet and a substrate body having a through hole provided on the base sheet.

  A cover member 5 is provided on the first main surface 4 a of the substrate 4. The cover member 5 is provided so as to close the recess 4 c of the substrate 4. The collection unit 3 is configured by the cover member 5 closing the recess 4 c of the substrate 4. In the present embodiment, the first flow path 2a and the second flow path 2b are similarly configured by the cover member 5 closing the recess of the substrate 4.

  The cover member 5 can be made of, for example, a flexible material such as a resin film. As the resin film, for example, a thermoplastic resin such as a cycloolefin polymer, a cycloolefin copolymer, polycarbonate, polymethyl methacrylate, or polypropylene can be used.

  Further, the cover member 5 may be made of an elastic member. The elastic member is not particularly limited, but is preferably an elastomer. In the present invention, the substrate 4 and the cover member 5 may be integrally formed.

  The method of attaching the cover member 5 to the first main surface 4a of the substrate 4 is not particularly limited, and a known laminating method can be used. Examples of the laminating method include a method such as press bonding and roll lamination. The lamination conditions are not particularly limited, but, for example, it is preferable to laminate at a temperature of 50 ° C. or lower. Further, it is preferable that the lamination is performed under a pressure of 10 MPa or less. By laminating under such conditions, the embedding of the cover member 5 in the flow path 2 can be made more difficult to occur.

  In the substrate 4, a flow path 2 through which a fluid is sent is provided. Here, the flow channel 2 is a micro flow channel. The flow path 2 may be a flow path having a larger cross-sectional area than the micro flow path instead of the micro flow path. However, a microchannel is preferable. Thus, various analyzes can be performed with a small amount of sample.

  The cross-sectional shape and size of the microchannel are not particularly limited. For example, in the case of using a pump or gravity when flowing a fluid through the microchannel, from the viewpoint of reducing the channel resistance, when the cross-sectional shape of the microchannel is substantially rectangular (including square), it is small. The dimension of the other side is preferably 20 μm or more, more preferably 50 μm or more, and still more preferably 100 μm or more. From the viewpoint of further miniaturization of the microfluidic device using the analysis tool 1, the dimension of the smaller side is preferably 5 mm or less, more preferably 1 mm or less, and even more preferably 500 μm or less.

  Further, when the cross-sectional shape of the microchannel is substantially circular, the diameter (in the case of an ellipse, the minor axis) is preferably 20 μm or more, more preferably 50 μm or more, and even more preferably 100 μm or more. In light of the further miniaturization of the microfluidic device, the diameter (short diameter in the case of an ellipse) is preferably equal to or less than 5 mm, more preferably equal to or less than 1 mm, and still more preferably equal to or less than 500 μm.

  On the other hand, for example, when a capillary phenomenon is effectively used when flowing a fluid through a microchannel, when the cross-sectional shape of the microchannel is substantially rectangular (including a square), the dimension of the smaller side is determined. The thickness is preferably 5 μm or more, more preferably 10 μm or more, and further preferably 20 μm or more. Further, the dimension of the smaller side is preferably 200 μm or less, and more preferably 100 μm or less.

  In the present embodiment, the above-described collection unit 3 is provided in the middle of the flow path 2. The flow path 2 has a first flow path 2a and a second flow path 2b.

  The first flow path 2a is an upstream micro flow path provided on the upstream side of the collection unit 3. One end of the first flow path 2 a is connected to the collection unit 3. On the other hand, a fluid or gas can flow in from the inlet side where the other end of the first flow path 2a is provided. In the case where a plurality of fluids or gases are caused to flow into the recovery unit 3, they may all flow from the same inlet, or a separate inlet or first flow path 2a may be provided for each flowing fluid or gas. Good.

  The second flow path 2b is a downstream micro flow path provided downstream of the collection unit 3. One end of the second flow path 2b is connected to the collection unit 3. From the other end provided on the downstream side of the second flow path 2b, a fluid or gas can be sent to another portion. In addition, other flow paths than the first flow path 2a and the second flow path 2b may be connected as necessary. In addition, the shape of the flow path 2 is not particularly limited, and the flow path, the mixing / reaction unit, and the like can be arranged according to a target test and reaction.

  In the present embodiment, the recovery unit 3 is filled with the solid phase carrier 6 for supporting the target substance. Such a solid support 6 is not particularly limited, but for example, glass fiber, silica monolith, cellulose fiber and the like can be used. The solid support 6 is preferably a glass fiber or a silica monolith. One of these may be used alone, or a plurality of them may be used in combination. The solid phase carrier 6 can be punched and filled in the same shape as the collection unit 3, for example.

  The retained particle diameter of the solid support 6 is preferably 1 μm or less, more preferably 0.8 μm or less, further preferably 0.6 μm or less, and further preferably 0.1 μm or more. When the retained particle diameter of the solid support 6 is equal to or less than the upper limit, the recovery rate of the target substance can be more effectively increased. In addition, when the retained particle diameter of the solid phase carrier 6 is equal to or less than the upper limit, it is possible to more smoothly send the cleaning liquid or the like to the collection unit 3. The retained particle diameter of the solid support 6 refers to a particle diameter capable of retaining 90% or more when the dispersion water of the seven types of powder specified in JIS Z 8901 is naturally filtered.

  As shown in FIG. 1, in the present embodiment, the planar shape of the collection unit 3 is substantially circular. The planar shape of the collection unit 3 may be a substantially perfect circle or a substantially ellipse. In the present embodiment, the ratio φ / W1 and the ratio φ / W2 are in the range of 1.5 or more and 6 or less, respectively. Note that W1 is the width of the first flow path 2a. W2 is the width of the second flow path 2b. φ is the diameter of the collecting unit 3 when the shape of the collecting unit 3 is circular. Φ is (major axis + minor axis) / 2 when the shape of the collection unit 3 is elliptical.

  In the present embodiment, since the ratio φ / W1 and the ratio φ / W2 are respectively within the above ranges, it is possible to make it difficult to generate a residual liquid such as a cleaning liquid to be described later, and to increase the recovery rate of the target substance.

  When the ratio φ / W1 exceeds the upper limit, the washing liquid or the like cannot fill the entire solid phase carrier 6, and the recovery rate of the target substance may be deteriorated. When the ratio φ / W1 is smaller than the lower limit, the amount of the target substance adsorbed on the solid support may be insufficient, and the recovery may be deteriorated.

  When the ratio φ / W2 exceeds the above upper limit, it may be difficult to feed a cleaning liquid or the like. If the ratio φ / W2 is smaller than the lower limit, the liquid remaining on the solid phase carrier 6 increases, and the recovery rate may deteriorate.

  In order to further increase the recovery rate of the target substance, the ratio φ / W1 and the ratio φ / W2 are each preferably 2 or more, and more preferably 5 or less.

  In addition, W1 can be set to, for example, 0.15 mm or more and 5 mm or less. W2 can be, for example, 0.15 mm or more and 5 mm or less. φ can be, for example, 1 mm or more and 10 mm or less.

  As shown in FIG. 2, in the present embodiment, the first flow path 2 a, the second flow path 2 b, and the collection unit 3 are arranged so as to face the cover member 5. Therefore, by heating from the cover member 5 side with a heater or the like, the target substance can be more sufficiently dried. Therefore, the recovery rate of the target substance can be further increased. Note that, in the present invention, the first flow path 2a, the second flow path 2b, and the collection unit 3 do not necessarily have to be arranged so as to face the cover member 5.

  In the present invention, it is preferable that the depth D1 of the first flow path 2a, the depth D2 of the second flow path 2b, and the depth D3 of the collection unit 3 are equal. In this case, since the cleaning liquid or the like can be sent without remaining liquid, the recovery rate of the target substance can be further increased. However, the depth D1 of the first flow path 2a, the depth D2 of the second flow path 2b, and the depth D3 of the collection unit 3 do not have to be equal, and are not particularly limited.

  The depths of the first flow path 2a, the second flow path 2b, and the collecting unit 3 are not particularly limited, but are preferably 1 mm or less, and more preferably 0.7 mm or less. The lower limits of the depths of the first flow path 2a, the second flow path 2b, and the collection unit 3 are not particularly limited, but may be, for example, 0.1 mm.

  In the present invention, the thickness of the cover member 5 is preferably 200 μm or less, more preferably 100 μm or less. When the thickness of the cover member 5 is equal to or less than the above upper limit, the target substance can be more reliably dried by heating from the cover member 5 side with a heater or the like. Note that the lower limit of the thickness of the cover member 5 can be, for example, 50 μm. When the thickness of the cover member 5 is equal to or more than the lower limit, the cover member 5 can be made more difficult to be torn, and the outflow of the reagent to the outside can be further suppressed.

  In the present invention, as shown in FIG. 3, in plan view, the intersection P of the extension line X1 along the direction in which the first flow path 2a extends and the extension line X2 in the direction in which the second flow path 2b extends is , It is preferable to be located so as to overlap with the center of gravity G of the collection unit 3. In this case, it is possible to make it more difficult for the cleaning liquid to remain, and to further increase the recovery rate of the target substance.

  Note that the extension line X1 along the direction in which the first flow path 2a extends passes through the center of the first flow path 2a in the width direction. The extension line X2 along the direction in which the second flow path 2b extends passes through the center of the second flow path 2b in the width direction.

  In the present invention, for example, the liquid sent to the recovery unit 3 can be sent by a liquid sending unit provided inside or outside the analysis tool 1. The liquid sending means is not particularly limited, and includes, for example, a micropump. Specifically, a means for sending a liquid, air, or a predetermined gas to the first flow path 2a using a micropump to send the liquid to the collection unit 3 side may be used. In this case, the micropump may be provided inside the analysis tool 1 or may be provided outside the analysis tool 1. By such means, the liquid is sent to the recovery unit 3.

  Further, as another liquid sending means, a gas generating member arranged in a space connected to the upstream side of the first flow path 2a can be cited. The gas generating member is a member that generates gas by an external force such as light or heat. Gas can be generated by applying an external force to the gas generating member at a predetermined timing, and the gas can be sent into the first flow path 2a. Thereby, the liquid can be sent from the first flow path 2a to the recovery unit 3 side. Examples of the gas generating member include a gas generating tape.

(Second embodiment)
FIG. 4 is a schematic plan view showing a main part of the analysis tool according to the second embodiment of the present invention. FIG. 5 is a schematic cross-sectional view of a portion along the line CC in FIG. In FIG. 4, the inside of the analysis tool 21 is indicated by a broken line.

  In the first embodiment, the direction in which the first flow path 2a extends and the direction in which the second flow path 2b extends are different directions, whereas in the analysis tool 21 of the second embodiment, the first direction is different. The direction in which the flow path 2a extends is the same as the direction in which the second flow path 2b extends. The depth D1 of the first flow path 2a, the depth D2 of the second flow path 2b, and the depth D3 of the collection unit 3 are made equal. The width W1 of the first flow path 2a is equal to the width W2 of the second flow path 2b. The other points are the same as in the first embodiment.

  Also in the analysis tool 21 of the second embodiment, since the ratio φ / W1 and the ratio φ / W2 shown in FIG. 1 are respectively within the above ranges, it is possible to make it difficult to generate a liquid residue such as a cleaning liquid and the like. Recovery rate can be increased.

  Further, as shown in the first embodiment and the second embodiment, the direction in which the first flow path 2a extends and the direction in which the second flow path 2b extends may be the same direction, and may be different directions. It may be. However, in a plan view, the intersection of the extension line along the direction in which the first flow path 2a extends and the extension line in the direction in which the second flow path 2b extends is positioned so as to overlap the center of gravity of the collection unit 3. Is preferred. In this case, the remaining of the cleaning liquid is less likely to occur, and the recovery rate of the target substance can be further increased.

[Washing method]
Hereinafter, an example of a method for cleaning a target substance using the above-described analysis tool 1 will be described.

  In the cleaning method of the present embodiment, first, the liquid containing the target substance is sent from the first flow path 2a to the collection unit 3. As a result, the target substance is supported on the solid support 6. Next, the cleaning liquid is sent from the first flow path 2a to the collection unit 3. Thereby, the target substance carried on the solid support 6 is washed. Next, the target substance is dried by heating the recovery unit 3.

  The dried target substance can be recovered by sending the recovered liquid from the first flow path 2a to the recovery unit 3.

  The liquid containing the target substance, the cleaning liquid, and the recovery liquid can be respectively sent by the above-described liquid sending means.

  As described above, in the cleaning method according to the present embodiment, since the analysis tool 1 is used, the liquid such as the cleaning liquid hardly remains. Therefore, the recovery rate of the target substance can be increased.

  In addition, when heating the recovery unit 3, it can be heated by a heater, a Peltier element, or the like. At this time, it is desirable to heat the collection unit 3 from the cover member 5 side. In that case, the target substance can be more reliably dried, and the recovery rate of the target substance can be further increased.

  Hereinafter, the present invention will be clarified by giving specific examples and comparative examples of the present invention. Note that the present invention is not limited to the following embodiments.

(Examples 1 to 52, Comparative Examples 1 to 18)
In Examples 1 to 52 and Comparative Examples 1 to 18, the analysis tool 1 shown in FIGS. 1 and 2 was manufactured as follows. The width W1 and depth D1 of the first flow path 2a, the width W2 and depth D2 of the second flow path 2b, and the diameter φ and depth D3 of the collection unit 3 are shown in Tables 1 to 4 below. , And may be different from FIGS. 1 and 2 in some cases.

  As a material constituting the substrate 4, a cycloolefin polymer (trade name "Zeonor 1060R" manufactured by Zeon Corporation) was used, and the substrate 4 having the concave portion 4c was produced by injection molding. The analysis tool 1 was produced by using a sealing tape (trade name “ZF14-188” manufactured by Zeon Corporation) as the cover member 5 and closing the recess 4 c of the substrate 4 with the sealing tape. In Example 43, a sealing tape (trade name “ZF14-100” manufactured by Zeon Corporation) was used as the cover member 5, and in Example 44, a sealing tape (trade name “ZF14” manufactured by Zeon Corporation) was used. -060 ") was used.

  In a plan view, the intersection P of the extension line X1 along the direction in which the first flow path 2a extends and the extension line X2 along the direction in which the second flow path 2b extends overlaps the center of gravity G of the collection unit 3. Was.

  The planar shape of the collecting unit 3 is a circle, and the diameter φ and the depth D3 of the collecting unit 3 and the material of the solid support 6 are as shown in Tables 1 to 4 below. The glass fibers were GA200 (manufactured by Whatman, holding particle diameter 0.8 μm), GA201 (manufactured by Whatman, holding particle diameter 0.6 μm), GB140 (manufactured by Whatman, holding particle diameter 0.4 μm), GD121 ( QM-A (manufactured by Whatman, 2.2 μm) was used. As the silica monolith, a monolith (manufactured by GL Sciences Inc.) in a spin column for MonoFas (registered trademark) DNA purification kit I was used. In addition, a paper disk (manufactured by Advantech) was used as the cellulose fiber.

  The width W1, depth and φ / W1 of the first flow path 2a, and the width W2, depth and φ / W2 of the second flow path 2b are as shown in Tables 1 to 4 below.

  The target substances used were those shown in Tables 1 to 4 below.

Determination of recovery;
In Examples 1 to 52 and Comparative Examples 1 to 18, the recovery rates were measured as follows.

  First, a gas was generated by the first micropump, and the gas was applied from the back of a liquid containing an objective nucleic acid (aqueous solution containing guanidine hydrochloride). By applying the gas, the liquid containing the target substance was pressed from the first flow path 2a to the recovery section 3 so that the target substance was supported on the solid phase carrier 6 of the recovery section 3.

  After sending the liquid containing the target substance, gas was generated by the second micropump, and the gas was applied from behind the cleaning liquid (a 70% aqueous solution of ethanol). By pressing the cleaning liquid by applying gas, the target substance carried on the recovery unit 3 was cleaned. Subsequently, the heater was brought into contact with the cover member 5 side of the substrate 4 to dry the cleaning liquid at 100 ° C. for 5 minutes.

  Next, a gas was generated by the third micropump, and the gas was applied from behind the recovered liquid (water). By applying a gas from the rear of the collected liquid and pressing the collected liquid, the collected liquid was sent to the collecting unit 3, and the target substance carried on the collecting unit 3 was separated and collected.

  Next, the collected target substance was heated and cooled in a plurality of temperature ranges (95 ° C., 55 ° C., and 72 ° C.) for performing PCR. Thereby, the nucleic acid was amplified by the PCR reaction. After amplifying the nucleic acid, the amplified nucleic acid was detected by a method using a Q probe. The Ct value was calculated from the obtained amplification curve by using the Crossing Point method as an analysis method. In addition, the Ct value of the target substance having a known concentration was calculated, and the recovery rate (%) was calculated from the Ct value of each sample based on the Ct value.

[Evaluation criteria]
◎: Recovery rate of 70% or more ○: Recovery rate of 50% or more, less than 70% ×: Recovery rate of less than 50%

  The results are shown in Tables 1 to 4 below.

Reference numerals 1, 21 Analysis tool 2 Flow path 2a First flow path 2b Second flow path 3 Collection unit 4 Substrates 4a and 4b First and second main surfaces 4c Depression 5 Cover member 6 ... Solid phase carrier

Claims (11)

A flow path through which the fluid is sent;
A collection unit that is provided in the middle of the flow path and is filled with a solid phase carrier for collecting and washing a target substance by a solid phase extraction method,
With
The flow path has a first flow path connected to an upstream side of the recovery unit, and a second flow path connected to a downstream side of the recovery unit,
A planar shape of the collection unit is substantially circular;
In a plan view, the width of the first flow path is W1, the width of the second flow path is W2, and the diameter of the recovery section when the shape of the recovery section is circular, or the recovery section. Is an elliptical shape, when (major axis + minor axis) / 2 is φ, the ratio φ / W1 and the ratio φ / W2 are in the range of 1.5 or more and 6 or less, respectively. Analytical tools.   In a plan view, the intersection of the extension line along the direction in which the first flow path extends and the extension line in the direction in which the second flow path extends is located so as to overlap the center of gravity of the collection unit. The analysis tool according to claim 1. A substrate having a recess,
A cover member provided on the substrate and closing the recess of the substrate;
With
The analysis tool according to claim 1, wherein the collection unit is configured by closing the concave portion of the substrate with the cover member.   The analysis tool according to claim 3, wherein the first flow path, the second flow path, and the collection unit are arranged so as to face the cover member.   The analysis tool according to any one of claims 1 to 4, wherein the first flow path, the second flow path, and the recovery unit have the same depth.   The analysis tool according to any one of claims 3 to 5, wherein the cover member is made of a resin film.   The analysis tool according to claim 6, wherein the thickness of the resin film is 200 µm or less.   The analysis tool according to any one of claims 1 to 7, wherein the target substance is a nucleic acid.   The analytical device according to any one of claims 1 to 8, wherein the solid support is a glass fiber or a silica monolith. A method for cleaning the target substance using the analysis tool according to any one of claims 1 to 9,
Sending a liquid containing the target substance from the first flow path to the recovery section, and supporting the target substance on the solid phase carrier;
A step of sending a washing liquid from the first flow path to the collecting section, and washing the target substance carried on the solid phase carrier;
A step of drying the target substance by heating the recovery unit;
A cleaning method comprising:   The analysis tool includes a substrate having a concave portion, and a cover member provided on the substrate and closing the concave portion of the substrate, wherein the concave portion of the substrate is closed by the cover member. The cleaning method according to claim 10, wherein the recovery section is configured to perform the heating of the recovery section from the cover member side.

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