CN211645248U - PCR (polymerase chain reaction) rapid reaction chip - Google Patents

Abstract

The utility model relates to a PCR fast reaction chip, including the channel piece and the gasket that hugs closely with it, the channel piece is a film piece, including at least a set of PCR reaction group that comprises liquid feeding hole, exhaust hole and the PCR micro-reaction tank that is linked together with liquid feeding hole, exhaust hole, liquid feeding hole and exhaust hole run through the channel piece, liquid feeding hole communicates with PCR micro-reaction tank through liquid feeding channel, the exhaust hole communicates with PCR micro-reaction tank through exhaust channel; the PCR micro-reaction tank, the liquid adding channel and the exhaust channel are single-sided hollow structures which are open towards a sealing sheet, and one side of the sealing sheet is tightly combined with one side of the channel sheet, which is provided with the hollow structures, to form a cavity; the sealing sheet is made of a metal film or a biological film. The PCR rapid reaction chip structure can be matched with a matched heating device to realize rapid temperature rise and fall.

Description

PCR (polymerase chain reaction) rapid reaction chip

Technical Field

The utility model relates to the field of biology and analytical chemistry, in particular to a PCR rapid reaction chip.

Background

PCR (polymerase chain reaction) is an important means for molecular biological research, the reaction time is always a limiting factor of PCR reaction, a conventional PCR reaction usually takes one or two hours, and the application field of the PCR reaction is limited by the long detection time in clinical detection, especially for emergency treatment. Along with the research and development and application of the PCR rapid reagent, the bottleneck of the reaction time is mainly focused on the temperature rising and reducing speed, and on the basis, how to increase the temperature rising and reducing speed of the PCR reaction becomes the key for expanding the application. In addition, the conventional PCR detection device often starts a new round of test after one round of test is finished, and in emergency application, the demand of follow-up test is increasing, and the follow-up function of the PCR device is also more and more important, but the current PCR rapid reaction chip cannot meet the demand.

Disclosure of Invention

In order to solve the problem, the utility model provides a PCR reaction chip, its structure setting can be supporting heating device and realize going up and down the temperature fast, shortens PCR reaction time.

The utility model adopts the technical proposal that:

a PCR fast reaction chip comprises a channel sheet and a sealing sheet closely attached to the channel sheet, wherein the channel sheet is a thin film sheet and comprises at least one PCR reaction group consisting of a liquid adding hole, an exhaust hole and a PCR micro reaction pool communicated with the liquid adding hole and the exhaust hole, the liquid adding hole and the exhaust hole penetrate through the channel sheet, the liquid adding hole is communicated with the PCR micro reaction pool through a liquid adding channel, and the exhaust hole is communicated with the PCR micro reaction pool through an exhaust channel; one surface of the channel sheet is provided with a single-surface hollowed structure which forms the PCR micro-reaction pool, the liquid feeding channel and the exhaust channel and is open towards the sealing sheet, and one surface of the sealing sheet is tightly combined with one surface of the hollowed structure of the channel sheet to form a cavity; the sealing sheet is made of a metal film or a biological film.

The PCR rapid reaction chip also comprises a cover plate, wherein the cover plate is arranged on one surface of the channel plate, which faces away from the hollow structure, and the surface of the cover plate, which faces towards the channel plate, is tightly attached to the channel plate and seals the liquid adding holes and the exhaust holes.

The liquid adding channel and the exhaust channel are arranged in parallel, and the cover plate is tightly attached to the channel sheet by means of adhesion or adsorption connection and used for sealing a liquid adding hole and an exhaust hole added with a reaction system, so that pollution in the PCR process is avoided.

The channel sheet is made of transparent materials, so that optical detection is facilitated. Preferably, the material of the channel sheet is selected from any one of acrylic, glass, PE, PP and PS.

The thickness of the sealing piece is 0.01-0.1 mm. Preferably, the sealing plate is an aluminum foil, the aluminum has strong thermal conductivity, and heat can be quickly transferred to the sample in the PCR micro-reaction pool through the aluminum foil.

The combination mode of the channel piece and the sealing piece comprises welding, sticking or adsorption.

A reaction reagent system is preset in a PCR micro-reaction pool of the PCR reaction chip. Preferably, the system can be liquid, dry powder or freeze-dried ball, etc.

The liquid adding hole and the exhaust hole are arranged at one end of the channel sheet and are respectively connected with the PCR micro-reaction tank arranged at the other end of the channel sheet through a liquid adding channel and an exhaust channel; the centers of the PCR micro reaction pools are on a straight line, and the reaction groups are arranged in a parallel array structure.

Preferably, the liquid feeding channel and the exhaust channel are arranged in parallel, so that the space can be saved to the greatest extent, and more reaction groups can be distributed on a limited large PCR reaction chip. The cover plate is provided with bar code information for recording related information of a preset reagent system in the PCR reaction chip.

The thickness of the channel sheet in the PCR micro reaction pool area is larger than that of other areas, so that the volume of the micro reaction pool is increased, and more samples are contained.

Embedding a reaction reagent in a PCR micro-reaction pool of a PCR reaction chip in advance, wherein the preset reagent can be liquid, dry powder or freeze-dried balls and the like, the processed chip has a certain period of validity, and a sample can be added through a liquid adding hole only after proper temperature return during an experiment; when in use, the PCR reaction chip is manually swung along the outward direction of the PCR micro-reaction tank, so that the reaction system is transferred to the PCR micro-reaction tank.

The PCR reaction chip can be added with a sample or a premixed reaction system through the liquid adding hole, the added liquid can enter the PCR micro-reaction pool through the channel connected with the PCR micro-reaction pool through the liquid adding hole, and the air replaced by the liquid entering can be discharged from the air exhaust hole through the connecting channel.

The utility model discloses a gasket of PCR reaction chip is the heat conduction membrane that materials such as metal were made, and it directly becomes the partly of cavity, and it is fast than traditional PCR reaction pipe heat conduction, makes the faster heating and cooling of experiment system to accelerate PCR reaction process.

Drawings

FIG. 1 is a schematic diagram of a layered structure of a PCR reaction chip according to the present invention;

FIG. 2 is a schematic diagram of the structure of a PCR reaction chip according to the present invention;

FIG. 3 is a schematic front view (back side) of a channel sheet of a PCR reaction chip according to the present invention;

FIG. 4 is a schematic isometric view of a chip channel sheet for PCR reaction according to the present invention;

1-PCR reaction chip, 11-liquid adding hole, 12-air exhausting hole, 13-PCR micro reaction pool, 14-liquid adding channel, 15-air exhausting channel, 16-label, 17-cover plate, 18-sealing plate and 19-channel plate.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by manufacturers, and are all conventional products available on the market.

As shown in FIGS. 1 and 2, the PCR chip 1 is composed of a cover plate 17, a channel plate 19 and a sealing plate 18 from the top to the bottom, wherein the channel plate 19 is an intermediate layer. As shown in fig. 3 and 4, the channel sheet 19 is a thin film sheet, and includes at least one PCR reaction set composed of a liquid adding hole 11, an air discharging hole 12, and a PCR micro-reaction tank 13 communicated with the liquid adding hole 11 and the air discharging hole 12, the liquid adding hole 11 and the air discharging hole 12 penetrate through the channel sheet 19, the liquid adding hole 11 is communicated with the PCR micro-reaction tank through a liquid adding channel 14, and the air discharging hole 12 is communicated with the PCR micro-reaction tank 13 through an air discharging channel 15; the PCR micro-reaction pool 13, the liquid adding channel 14 and the exhaust channel 15 are of single-side hollow structures which are open towards the sealing sheet 18, and one side of the sealing sheet 18 is tightly combined with one side of the channel sheet, which is provided with the hollow structures, to form a cavity; the material of the sealing sheet 18 is selected from a metal film or a biological film. The liquid adding hole 11 and the exhaust hole 12 are arranged at one end of the channel sheet 19 and are respectively connected with the PCR micro reaction tank arranged at the other end of the channel sheet 19 through the liquid adding channel 14 and the exhaust channel 15; the centers of the PCR micro reaction pools 13 are on a straight line, and the reaction groups are arranged in a parallel array structure.

The cover plate 17 is arranged on one surface, back to the hollow structure, of the channel piece 19, and one surface, facing the channel piece 19, of the cover plate 17 is tightly attached to the channel piece and seals the liquid adding holes and the exhaust holes.

In some embodiments, the cover 17 may be adhered or attached to the channel piece to close the filling hole and the vent hole.

In some embodiments, the liquid adding channel 14 and the air exhausting channel 15 are disposed in parallel, and are used for sealing the liquid adding hole and the air exhausting hole added with the reaction system, so as to avoid pollution in the PCR process.

In some embodiments, the channel piece 19 is transparent to facilitate detection. The material of the channel sheet 19 is selected from any one of acryl, glass, PE, PP, and PS. The combination mode of the channel sheet and the sealing sheet comprises welding, sticking or adsorption.

In some embodiments, the sealing sheet 18 is an aluminum foil with a thickness of 0.01-0.1 mm and a high thermal conductivity, and can be bonded to the channel sheet 19. The cover plate 17 is also in a membrane structure and is adhered and connected with the channel plate 19, the cover plate 17 is separated from the channel plate 19 when in experiment, the PCR reaction system is added through the liquid adding hole 11 and exhausted through the exhaust hole 12, then the cover plate 17 is adhered with the channel plate 19, the liquid adding hole 11 and the exhaust hole 12 on the channel plate are sealed, the communication with the outside is avoided, and the pollution of the closed reaction system is reduced.

In other embodiments, the aluminum foil has a thickness of 0.01 to 0.05 mm.

In other embodiments, 3 reaction sets are provided on one PCR reaction chip 1, and 3 PCR reaction samples can be added simultaneously. The user opens the connection between the cover plate 17 and the channel plate 19, and can add the reaction system into the PCR reaction chip 1 through the liquid adding hole 11, the liquid adding hole 11 is connected with the PCR micro reaction tank 13 through the channel 14, and the exhaust hole 12 is connected with the PCR micro reaction tank 13 through the channel 15. After adding liquid, one side of the sample adding hole 11 of the PCR reaction chip 1 can be held by hand, one side of the PCR micro reaction tank 13 faces outwards, the PCR reaction chip 1 is swung, under the action of external force, a PCR reaction system moves to the PCR micro reaction tank 13 from the sample adding hole 11, meanwhile, gas in the PCR micro reaction tank 13 is discharged through the exhaust hole 12 connected with the PCR micro reaction tank, and then the cover plate 17 is covered, so that the sample adding hole 11 and the exhaust hole 12 are not communicated with the atmosphere.

In other embodiments, the material of the sealing sheet can be selected from other transparent or opaque biological sterile films or metal films which are easy to conduct heat, and the sealing sheet can obtain an extremely fast temperature rise and fall speed through contact conduction with the heating structure.

In some embodiments, a reaction reagent system, such as a liquid, a dry powder or a freeze-dried ball, is pre-set in a PCR micro-reaction chamber of a PCR reaction chip, and barcode information is attached to a cover plate for recording information related to the pre-set reagent system in the PCR reaction chip.

In other embodiments, the thickness of the channel sheet in the PCR micro reaction pool area is 1-3 mm greater than that of other areas, so that the volume of the micro reaction pool is increased by increasing the thickness of the PCR reaction pool, and more samples are contained.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims (9)

1. A PCR fast reaction chip is characterized by comprising a channel sheet and a sealing sheet closely attached to the channel sheet, wherein the channel sheet is a thin film sheet and comprises at least one PCR reaction group consisting of a liquid adding hole, an exhaust hole and a PCR micro reaction tank communicated with the liquid adding hole and the exhaust hole, the liquid adding hole and the exhaust hole penetrate through the channel sheet, the liquid adding hole is communicated with the PCR micro reaction tank through a liquid adding channel, and the exhaust hole is communicated with the PCR micro reaction tank through an exhaust channel; one surface of the channel sheet is provided with a single-surface hollowed structure which forms the PCR micro-reaction pool, the liquid feeding channel and the exhaust channel and is open towards the sealing sheet, and one surface of the sealing sheet is tightly combined with one surface of the hollowed structure of the channel sheet to form a cavity; the sealing sheet is made of a metal film or a biological film.

2. The PCR chip of claim 1, further comprising a cover plate disposed on a surface of the channel plate facing away from the hollow structure, wherein a surface of the cover plate facing the channel plate is closely attached to the channel plate and seals the liquid feeding holes and the air discharging holes.

3. The chip for PCR rapid reaction according to claim 2, wherein the liquid feeding channel and the air discharging channel are arranged in parallel, and the close attachment of the cover plate and the channel plate is achieved by adhesion or adsorption, and is used for sealing the liquid feeding hole and the air discharging hole added with the reaction system, thereby avoiding the pollution in the PCR process.

4. The chip for PCR rapid reaction according to claim 1, wherein the channel sheet is made of transparent material for easy detection.

5. The PCR chip of claim 1, wherein the combination of the channel plate and the sealing plate comprises soldering, pasting or adsorbing.

6. The PCR chip of claim 2, wherein a reaction reagent system is pre-arranged in the PCR micro-reaction chamber of the PCR chip.

7. The PCR chip for rapid reaction according to claim 1, wherein the liquid adding hole and the air discharging hole are provided at one end of the channel piece, and are connected to the PCR micro-reaction tank provided at the other end of the channel piece through a liquid adding channel and an air discharging channel, respectively; the centers of the PCR micro reaction pools are on a straight line, and the reaction groups are arranged in a parallel array structure.

8. The PCR chip of claim 6, wherein the cover plate has bar code information for recording the information related to the pre-set reaction reagent system in the PCR chip.

9. The PCR chip as claimed in claim 1, wherein the thickness of the channel sheet in the PCR micro reaction chamber region is greater than that in other regions, so as to increase the volume of the micro reaction chamber and accommodate a larger amount of sample.

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