WO2022088670A1 - Liquide biologique de réparation des cheveux préparé par mélange d'enzyme tg et de kératine/acide aminé - Google Patents

Liquide biologique de réparation des cheveux préparé par mélange d'enzyme tg et de kératine/acide aminé Download PDF

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Publication number
WO2022088670A1
WO2022088670A1 PCT/CN2021/097351 CN2021097351W WO2022088670A1 WO 2022088670 A1 WO2022088670 A1 WO 2022088670A1 CN 2021097351 W CN2021097351 W CN 2021097351W WO 2022088670 A1 WO2022088670 A1 WO 2022088670A1
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WO
WIPO (PCT)
Prior art keywords
keratin
enzyme
hair care
amino acid
hair
Prior art date
Application number
PCT/CN2021/097351
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English (en)
Chinese (zh)
Inventor
肖静
李子源
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齐鲁工业大学
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Publication date
Application filed by 齐鲁工业大学 filed Critical 齐鲁工业大学
Priority to JP2023526290A priority Critical patent/JP2023542250A/ja
Publication of WO2022088670A1 publication Critical patent/WO2022088670A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/002—Preparations for repairing the hair, e.g. hair cure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/12—Preparations containing hair conditioners
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

Definitions

  • the invention relates to the technical field of hair care, in particular to a biological hair care and repairing liquid with TG enzyme complexed keratin/amino acid.
  • the main cause of hair damage is that the bonds between the proteins in the hair are broken, which changes the characteristics of the hair, and it is easy to be frizzy and broken by external force.
  • the use of alkaline solutions and reducing agents will reduce weight and destroy the disulfide bonds of keratin.
  • the purpose of the present invention is to provide a kind of biological hair care and repair solution of TG enzyme complex keratin/amino acid, so as to solve the problems existing in the above-mentioned prior art, so that the toughness and damage resistance of the hair are significantly improved, and a good repair is realized. Effect.
  • the present invention provides following scheme:
  • the present invention provides a kind of biological hair care repairing liquid.
  • the raw materials include the following components:
  • the macromolecular hydrolyzed keratin and the small molecular hydrolyzed keratin are obtained by hydrolysis of keratin extracted from wool.
  • the enzymatic activity of the TG enzyme is 4700-4800 U/g.
  • TG enzyme activity is 5-25 U/mL.
  • the molecular weight of the macromolecule hydrolyzed keratin is 20KDa-30KDa, and the molecular weight of the small molecule hydrolyzed keratin is 0.8KDa-1.2KDa.
  • amino acid is L-lysine.
  • the present invention utilizes the high-efficiency catalytic properties of TG enzyme, and on the basis of additional keratin to form more disulfide bonds, directionally catalyzes more amide covalent cross-linking of keratin, thereby forming more covalent bonds; at the same time, the present invention On the basis of exogenous addition of traditional small molecular keratin, macromolecular hydrolyzed keratin is also added.
  • the keratin with different molecular weights can ensure sufficient cross-linking between the exogenous keratin and the protein groups exposed at the damaged hair strands, and, In the presence of amino acids, TG enzyme can also make the above components form a cross-linked protein network, thereby significantly improving the strength, damage resistance, mild and long-lasting effect of hair.
  • the invention can achieve good repairing effect on damaged hair by determining the components and contents and regulating the proportion of hydrolyzed keratin with different molecular weights, and the product process is simple and has wide application and promotion value.
  • Fig. 1 is the infrared spectrogram of damaged hair sample of the present invention, embodiment 3 and comparative examples 6, 7 repairing hair sample;
  • 1 is the damaged hair sample
  • 2 is the single protein repair sample of Comparative Example 7
  • 3 is the TG enzyme repair sample of Comparative Example 6,
  • 4 is the hair sample of Example 3 TG enzyme-keratin/amino acid combined repair;
  • Fig. 2 is the SEM image of damaged hair sample of the present invention, embodiment 3 repairing hair sample;
  • the macromolecular hydrolyzed keratin and the small molecular hydrolyzed keratin in the present invention are obtained by hydrolyzing the keratin extracted from wool.
  • Numbering Material name Content (unit: %) 1 macromolecule hydrolyzed keratin 0.5 2 Small molecule hydrolyzed keratin 0.5 3 TGase 0.3 4 L-Lysine 0.5
  • Numbering Material name Content (unit: %) 1 macromolecule hydrolyzed keratin 0.8 2 Small molecule hydrolyzed keratin 0.2 3 TGase 0.1 4 D-Lysine 1 5 Deionized water margin
  • Numbering Material name Content (unit: %) 1 macromolecule hydrolyzed keratin 0.6 2 Small molecule hydrolyzed keratin 0.4
  • Numbering Material name Content (unit: %) 1 macromolecule hydrolyzed keratin 0.7 2 Small molecule hydrolyzed keratin 0.6 3 TGase 0.4 4 L-Lysine 0.8 5 Deionized water margin
  • Example 3 The difference from Example 3 is that the formulation components do not contain macromolecular hydrolyzed keratin.
  • Example 3 The difference from Example 3 is that the formulation components do not contain small molecule hydrolyzed keratin.
  • Example 3 The difference from Example 3 is that the formula does not contain amino acids.
  • Example 3 The difference from Example 3 is that the added small molecule hydrolyzed keratin has a molecular weight of 0.2KDa-0.6KDa.
  • Example 3 The difference from Example 3 is that the amount of lysine added is 1.2%.
  • Example 3 The difference from Example 3 is that the formulation components only contain TG enzyme and deionized water.
  • Example 3 The difference from Example 3 is that the formulation components only contain macromolecular hydrolyzed keratin, small molecular hydrolyzed keratin and deionized water.
  • G is the moisture content, %
  • W is the mass of the residual sample after alkali treatment, g.
  • Example 1 5.27 ⁇ 0.51 Example 2 5.35 ⁇ 0.47 Example 3 5.14 ⁇ 0.31 Example 4 5.21 ⁇ 0.52 Comparative Example 1 6.87 ⁇ 0.47 Comparative Example 2 6.54 ⁇ 0.54 Comparative Example 3 6.08 ⁇ 0.17 Comparative Example 4 6.11 ⁇ 0.48 Comparative Example 5 5.57 ⁇ 0.51 Comparative Example 6 6.99 ⁇ 0.46 Comparative Example 7 8.19 ⁇ 0.63
  • the biological hair care and repair solution of the TG enzyme complexed keratin/amino acid of the present invention has a good repair effect on damaged hair.
  • 1 is the damaged hair sample
  • 2 is the single protein repair sample of Comparative Example 7
  • 3 is the TG enzyme repair sample of Comparative Example 6
  • 4 is the TG enzyme-keratin/amino acid combined repair sample of Example 3.
  • a is the damaged hair sample
  • b is the repaired hair sample of Example 3.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

L'invention concerne un liquide biologique de réparation des cheveux préparé par le mélange d'une enzyme TG et de kératine/acide aminé, se rapportant au domaine technique des soins capillaires. Le liquide biologique de réparation des cheveux comprend les matières premières suivantes en pourcentage de masse : 0,5 à 0,8 % de kératine hydrolysée macromoléculaire, 0,2 à 0,6 % de kératine hydrolysée micromoléculaire, 0,1 à 0,5 % d'enzyme TG, 0,5 à 1 % d'acide aminé, le reste étant de l'eau désionisée. L'utilisation de la caractéristique catalytique à haute efficacité de l'enzyme TG permet la formation d'un tissu protéique réticulé par l'enzyme TG et les cheveux endommagés sur la base d'une addition supplémentaire de kératine et d'acide aminé, de sorte que la force, la ténacité et la résistance aux dommages des cheveux sont remarquablement améliorées, et qu'un effet doux et durable est obtenu. En déterminant précisément les composants et le contenu de la formule, un bon effet réparateur sur les cheveux abîmés peut être obtenu, et le produit est simple dans son processus et présente une large gamme d'applications et de valeurs de production à grande échelle.
PCT/CN2021/097351 2020-10-28 2021-05-31 Liquide biologique de réparation des cheveux préparé par mélange d'enzyme tg et de kératine/acide aminé WO2022088670A1 (fr)

Priority Applications (1)

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JP2023526290A JP2023542250A (ja) 2020-10-28 2021-05-31 Ｔｇ酵素複合ケラチン／アミノ酸の生物学的ヘアケア修復液

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CN202011171973.3 2020-10-28
CN202011171973.3A CN112263500B (zh) 2020-10-28 2020-10-28 一种tg酶复合角蛋白/氨基酸的生物护发修复液

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116115514A (zh) * 2023-04-04 2023-05-16 广州美思生物技术有限公司 一种护发的植物蛋白组合物及其制备方法与应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112263500B (zh) * 2020-10-28 2022-11-15 齐鲁工业大学 一种tg酶复合角蛋白/氨基酸的生物护发修复液

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CN116115514A (zh) * 2023-04-04 2023-05-16 广州美思生物技术有限公司 一种护发的植物蛋白组合物及其制备方法与应用

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