WO2022081124A1 - Dispositifs d'écoulement à effet de mèche automatique - Google Patents

Dispositifs d'écoulement à effet de mèche automatique Download PDF

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Publication number
WO2022081124A1
WO2022081124A1 PCT/US2020/055211 US2020055211W WO2022081124A1 WO 2022081124 A1 WO2022081124 A1 WO 2022081124A1 US 2020055211 W US2020055211 W US 2020055211W WO 2022081124 A1 WO2022081124 A1 WO 2022081124A1
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WIPO (PCT)
Prior art keywords
self
blocking
particulates
wicking
porous substrate
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Application number
PCT/US2020/055211
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English (en)
Inventor
Adam C. WEISMAN
Original Assignee
Hewlett-Packard Development Company, L.P.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Hewlett-Packard Development Company, L.P. filed Critical Hewlett-Packard Development Company, L.P.
Priority to PCT/US2020/055211 priority Critical patent/WO2022081124A1/fr
Publication of WO2022081124A1 publication Critical patent/WO2022081124A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow

Definitions

  • Self-wicking flow devices are intended to detect the presence of a10 target analyte in a liquid sample. These devices are simple to use and do not require specialized training of the user. Accordingly, self-wicking flow devices are widely used for medical diagnostic testing, environmental sample testing, and in laboratories, to name a few.
  • FIG.1 graphically illustrates a method of manufacturing a fluid barrier for a self-wicking flow device in accordance with the present disclosure
  • FIG.2 graphically illustrates a schematic view of an example20 self-wicking flow device in accordance with the present disclosure
  • FIG.3 graphically illustrates a schematic view of an example blocking particulate in accordance with the present disclosure
  • FIG.4 graphically illustrates a schematic view of an example self-wicking flow device with various fluid barriers in accordance with the present25 disclosure
  • FIG.5 graphically illustrates a schematic view of an example flow immunoassay system in accordance with the present disclosure.
  • Self-wicking flow devices can permit the detection of a target analyte in a sample fluid.
  • These devices can incorporate a porous substrate.
  • the 5 sample fluid sequentially runs along the porous substrate via capillary flow.
  • the target analyte will interact with a compound having a functional group that is capable of binding to the target analyte.
  • a testing region on the porous substrate can detect a complex of the target analyte and the compound and a control region on the porous substrate10 can detect the compound.
  • an optical indicator will appear or will not appear, depending on the device type.
  • Fluidic routing in self-wicking, lateral or directional flow assays can be used to improve the overall15 functionality of the device. For example, fluidic routing can improve mixing, allow for sequential additions of reagents (such as the compound having the functional group capable of binding to the target analyte), and limit bed volume in the testing region and the control region; thereby, improving detection sensitivity.
  • reagents such as the compound having the functional group capable of binding to the target analyte
  • limit bed volume in the testing region and the control region thereby, improving detection sensitivity.
  • the method can include associating blocking particulates having magnetic properties with a porous substrate of the self-wicking flow device, and repositioning the blocking particulates associated with the porous substrate using a magnet to cause the blocking particle to modulate fluid flow.
  • the blocking particulates can25 have an average particle size smaller than an average pore size of pores of the porous substrate.
  • the associating can occur by printing the blocking particulates in an aqueous liquid vehicle onto the porous substrate.
  • the blocking particulates can include a latex, a wax, or a combination thereof and the repositioning can occur in a direction opposite a30 magnetic field generated by the magnet.
  • the blocking particulates can include a magnetic particle core with a hydrophobic shell and dispersant thereon or the blocking particulates can include a magnetic particle core with a hydrophilic shell thereon and the repositioning can occur in a direction of a magnetic field generated by the magnet.
  • the method can further include adjusting an orientation of the magnet, adjusting a strength of a 5 magnetic field generated by the magnet, or a combination thereof.
  • the method can further include fusing the blocking particulates after the repositioning to form a permanent fluid barrier on the porous substrate.
  • the fusing can occur by melting a portion of the blocking particulates to fuse adjacent blocking particulates to one another, by crosslinking a polymer of a10 hydrophobic shell of the blocking particulates to one another, by crosslinking a polymer of a hydrophilic shell of the blocking particulates, or a combination thereof.
  • the method can further include alternating the associating and the fusing of the blocking particulates as the fluid barrier is being formed.
  • a self-wicking flow device (“device”).
  • the device can include a fluid flow channel including a porous substrate associated with blocking particulates that can have magnetic properties and can have an average particle size smaller than an average pore size of pores of the porous substrate.
  • the device can further include a fluid barrier that can include blocking20 particulates that can be repositioned relative to the porous substrate in response to a magnetic field.
  • the blocking particulates can include a magnetic particulate core with a hydrophobic shell and dispersant thereon or the blocking particulates can include a magnetic particulate core with a hydrophilic shell thereon.
  • the magnetic particulate core can include iron25 oxide, doped iron oxides, iron, nickel, cobalt, steel, stainless steel, gadolinium, samarium, neodymium, or alloys or combinations thereof.
  • the porous substrate can include a nitrocellulose pad, a cellulose pad, or a fiberglass pad, and an average pore size of the pores of the porous substrate can range from about 500 nm to about 50 ⁇ m.
  • the fluid30 barrier can be in the shape of a fluidic routing barrier, a bed volume fluidic routing barrier, a fluidic mixing barrier, or a combination thereof.
  • a flow immunoassay system can include a self-wicking flow device and a magnet.
  • the self-wicking flow device can include a fluid flow channel including a porous substrate and blocking particulates.
  • the blocking particulates can exhibit magnetic properties and can be repositionable in 5 response to a magnetic field to form a fluid barrier to modulate fluid flow.
  • the blocking particulates can also have an average particle size smaller than an average pore size of pores of the porous substrate.
  • the magnet can be associated with the self-wicking, lateral or directional flow device to generate a magnetic field to reposition the blocking particulates within the self-wicking flow10 device.
  • the self-wicking, lateral or directional flow device can further include a support in the form of a housing or a backing card.
  • the magnet can be embedded within or attached to the support.
  • FIG.1 depicts a method of manufacturing a fluid barrier for a self-wicking flow device (“method”).
  • the method 100 can include30 associating 110 blocking particulates having magnetic properties with a porous substrate of the self-wicking flow device, and repositioning 120 the blocking particulates associated with the porous substrate using a magnet to cause the blocking particle to modulate fluid flow.
  • the blocking particulates can have an average particle size smaller than an average pore size of pores of the porous substrate.
  • the method of manufacturing the fluid barrier can occur while 5 manufacturing a self-wicking flow device or may be used to modify an existing self-wicking flow device.
  • the associating 110 can occur by applying blocking particulates to the porous substrate.
  • “applying” may refer to placement of blocking particulates on a porous substrate.
  • the applying in some10 examples can occur by placement of dry blocking particulates onto the substrate and in yet other examples, the blocking particulates may be dispersed in an aqueous liquid vehicle to form a blocking fluid.
  • the blocking particulates may be dispersed in and carried by an aqueous liquid vehicle in the form of a blocking particulate dispersion.
  • the aqueous liquid vehicle can be15 water.
  • the aqueous liquid vehicle can include water and other components, as described in greater detail below.
  • applying may refer to any technology that can be used to put or place the blocking particulate dispersion on the porous substrate.
  • “applying” of the blocking20 particulate dispersion may refer to “jetting,” “ejecting,” “dropping,” “spraying,” or the like.
  • jetting or “ejecting” refers to expelling of a blocking particulate dispersion from ejection or jetting architecture, such as ink-jet architecture.
  • Ink-jet architecture can include thermal or piezoelectric architecture.
  • such architecture can be configured to print varying drop sizes such25 as from about 3 picoliters to less than about 10 picoliters, or to less than about 20 picoliters, or to less than about 30 picoliters, or to less than about 50 picoliters, etc.
  • the associating can occur by dropping the blocking particle dispersion from an applicator, such as a pipette.
  • the associating can occur by printing the blocking particulate dispersion onto the30 porous substrate, such as via ink-jet architecture. Associating the blocking particulate dispersion using inkjet architecture may permit control in the placement area and quantity of the blocking particulates applied to the porous substrate.
  • repositioning 120 of the blocking particulates may be via the use of a magnet.
  • the magnet can 5 influence a position of the blocking particulates by creating a magnetic field which attracts or repels the blocking particulates.
  • the blocking particulates may include a latex, a wax, or a combination thereof. Latexes and waxes are diamagnetic materials. Diamagnetic material may be repulsed by a magnetic field and may orient in a location opposite the magnetic field generated10 by a magnet.
  • the blocking particulates may include a magnetic particle core with a hydrophobic shell and dispersant thereon or a magnetic particle core with a hydrophilic shell thereon.
  • a geometry and strength of the magnetic field generated by the magnet can attract or repel the blocking particulates and can permit a manufacturer to control a position of the blocking particulates associated with the porous substrate.
  • the method can further include adjusting an orientation of the magnet, a strength of a magnetic field generated by the magnet,20 or a combination thereof. Adjusting an orientation and/or strength may permit repositioning 120 of the blocking particulates.
  • the magnet can be a permanent magnet attached to the self-wicking flow device or can be a removable magnet separate of the device.
  • a permanent magnet may be used to continuously apply a magnetic field and may hold the blocking particulates in a desired position without25 fusing.
  • the blocking particulates may be fused after repositioning 120.
  • the fusing can occur by melting a portion of the blocking particulates to fuse adjacent blocking particulates to one another, by crosslinking a polymer of a hydrophobic shell of the blocking particulates to one another, by30 crosslinking a polymer of a hydrophilic shell of the blocking particulates to one another, or a combination thereof.
  • the fusing can occur by melting a portion of the blocking particulates. Melting a portion of the blocking particulates can cause individual blocking particles to fuse, bind, cure, etc.
  • the melting can occur by selective application of heat.
  • the melting can involve positioning the porous substrate in 5 or near a heat generating source, such as a light, an oven, a flame, a laser, or the like.
  • fusing can occur by crosslinking the blocking particulates to one another.
  • a portion of the hydrophobic shell or the hydrophilic shell of the blocking particulates can include a crosslinking agent.
  • The10 crosslinking agent may crosslink with crosslinking agents on adjacent blocking particulates or may crosslink to another material of the blocking particulates.
  • the blocking particulates may include initiation sites that may be activated to polymerize monomers on a surface of adjacent blocking particulates to one another.
  • the polymerization can occur post-orientation.
  • polymerization may be initiated chemically, radiantly, thermally, magnetic oscillations, or a combination thereof.
  • Fusing the blocking particulates to one another may allow for the formation of a permanent fluid barrier. Following formation of the permanent fluid barrier, the magnetic field and/or the magnet generating the magnetic field may20 be removed from the area near the porous substrate.
  • the method can include alternating the associating 110 and the fusing of the blocking particulates while forming the fluid barrier.
  • FIGS.2-430 depict various self-wicking flow devices and/or components thereof. These devices can be relevant to the methods and systems described herein, as they can be used in the context of the methods and systems. These various examples can include various features, with several features common from example to example.
  • a self-wicking flow device 200 can include a fluid flow channel including a porous substrate 210 associated10 with blocking particulates 220 having magnetic properties.
  • the blocking particulates may have an average particle size smaller than an average pore size of pores of the porous substrate.
  • the self-wicking flow device may further include a fluid barrier 230 including the blocking particulates that can be repositioned relative to the porous substrate in response to a magnetic field.
  • the fluid flow channel can include a negative space that can be etched, molded, or engraved from a material of a housing and may surround the porous substrate of a self-wicking flow device.
  • the fluid flow channel can be sized and shaped to surround the porous substrate.
  • the fluid flow channel may include a pathway.
  • the pathway may be a linear pathway, a curved path, a pathway with20 turns, a branched pathway, a serpentine pathway, or any other pathway configuration. In some examples, the pathway may be linear and/or branched.
  • the porous substrate may be present in a portion of or throughout the entire length of the fluid flow channel.
  • the porous substrate may include a substrate that can allow an25 analyte fluid to flow therethrough via capillary action.
  • the porous substrate can include nitrocellulose, cellulose, acetate cellulose, fiberglass, porous silica, polyester, surface modified polyester, hydrogel, nylon, polytetrafluorethylene, silica, or a combination thereof.
  • the porous substrate can include a nitrocellulose pad, a cellulose pad, or a fiberglass30 pad.
  • the porous substrate can include a nitrocellulose pad.
  • Pores of the porous substrate can have an average pore size ranging from about 500 nm to about 50 ⁇ m, from about 1 ⁇ m to about 50 ⁇ m, from about 5 ⁇ m to about 50 ⁇ m, from about 15 ⁇ m to about 30 ⁇ m, from about 500 nm to about 5 ⁇ m, or from about 2 ⁇ m to about 8 ⁇ m.
  • a thickness of the porous substrate can range from about 50 ⁇ m to about 500 ⁇ m, from about 250 ⁇ m to about 500 ⁇ m, or 5 from about 50 ⁇ m to about 300 ⁇ m.
  • a length of the porous substrate can range from about 10 mm to about 50 mm, from about 10 mm to about 30 mm, or from about 25 mm to about 50 mm.
  • a width of the porous substrate can range from about 3 mm to about 10 mm, from about 4 mm to about 8 mm, or from about 3 mm to about 8 mm.
  • the porous membranes will have rectangular dimensions ranging from 50-500 microns (height), 10-50mm (length), and 3-10mm (width) for lateral flow devices.
  • the magnetic barriers will further restrict the dimensions of where the fluid may flow to a minimum of 10 microns (up to the maximum dimension of the substrate) in any axis depending on the method of application15 and the magnetic field.
  • the porous substrate can include a complexing region, a fluid flow region, and a detecting region.
  • the complexing region can include a compound having a functional group to bind with an analyte in a sample.
  • the compound having a functional group to bind with the analyte in the sample may vary based20 on the analyte and the purpose of the self-wicking flow device.
  • the compound having the functional group to bind with the analyte in the sample can include colloidal gold, a colored latex particle, a fluorescent latex particle, a paramagnetic latex particle, a cellulose nanobead, or a combination thereof.
  • the fluid flow region can be a portion of the porous substrate positioned between the25 complexing region and the detecting region.
  • a length of this region can vary based on the amount of time desired for the fluid to pass from the complexing region to the detecting region.
  • the detecting region can include a test strip and a control strip.
  • the detecting region may be a sandwich format detecting region or a competitive format detecting region.
  • a sandwich format detecting region can30 generate a positive result by displaying an optical indicator, such as a colored line.
  • a competitive format detecting region can generate a positive result by displaying the absence of an optical indicator.
  • the porous substrate may further include a flow controlling agent.
  • the flow controlling agent may be impregnated within the porous substrate and may include buffer salts, proteins, surfactants, and the like. 5 [0027]
  • the blocking particulates can include particulates having magnetic properties.
  • the blocking particulates 220 can include a magnetic particulate core 222 with a hydrophobic shell 224 and a dispersant thereon 226. See FIG.3.
  • the blocking particulates can include particulates having a magnetic particulate core with a hydrophilic shell thereon.
  • the magnetic particulate core can include a solid magnetic core.
  • the magnetic particulate core can include several smaller particulate magnetic particles, as shown in FIG.3.
  • the magnetic particulate core may include iron oxide, doped iron oxide, iron, nickel, cobalt, steel, stainless steel, gadolinium, samarium, neodymium, or alloys or combinations thereof.
  • the magnetic particulate core may include iron oxide, doped iron oxide, or alloys or combinations thereof. In yet another example, the magnetic particulate core may include a doped iron oxide, and the dopant may include zinc, yttrium, manganese, or a combination thereof.
  • An average particle size of the magnetic particulate core can range20 from about 10 nm to about 5 ⁇ m. In yet other examples, the magnetic particulate core can range from about 10 nm to about 500 nm, from about 10 nm to about 200 nm, from about 100 nm to about 300 nm, from about 250 nm to about 500 nm, or from about 1 ⁇ m to about 5 ⁇ m.
  • particle size can refer to the value of the diameter of spherical particles or in particles that are not spherical25 can refer to the longest dimension of that particle.
  • Average particle size can be measured using a particle analyzer such as the MASTERSIZERTM 3000 available from Malvern Panalytical.
  • the particle analyzer can measure particle size using laser diffraction. A laser beam can pass through a sample of particles and the angular variation in intensity of light scattered by the particles can be measured. 30 Larger particles scatter light at smaller angles, while small particles scatter light at larger angles.
  • the particle analyzer can then analyze the angular scattering data to calculate the size of the particles using the Mie theory of light scattering.
  • the particle size can be reported as a volume equivalent sphere diameter.
  • particle size can be determined and/or confirmed using a scanning electron microscope (SEM).
  • a deviation in particle size 5 between individual magnetic particulate cores can be less than about 20%, less than about 10%, less than about 7%, or less than about 5%.
  • magnetic particulate cores with a target of 100 nm in size can have a magnetic core particle size that can range from about 90 nm to about 110 nm, from about 80 nm to about 120 nm, from about 90 nm to about 110 nm, from about 93 nm to10 about 107 nm, or from about 95 nm to about 105 nm, for example.
  • the hydrophobic or hydrophilic shell can include paraffin, polyolefin, polytetrafluoroethylene, silica, polystyrene, latex, or a combination thereof.
  • the hydrophobic or hydrophilic shell can include latex and the latex may include polymers and copolymers of acrylic, vinyl acetate,15 polyester, vinylidene chloride, butadiene, styrene-butadiene, acrylonitrile-butadiene, acrylol, or a combination thereof.
  • the hydrophobic or hydrophilic shell may have a melting point above ambient temperatures (about 20 °C to about 25 °C).
  • the blocking particulates can include a20 hydrophilic shell. Blocking particulates with a hydrophilic shell can be self-dispersing. In other examples, the blocking particulates can include a hydrophobic shell. The hydrophobic shell can have a dispersant disposed thereon.
  • the dispersant may include polyvinyl alcohol, polyvinyl alcohol cross-linked with boric acid, styrene-acrylate polymers, styrene-acrylate 25 copolymers, styrene-acrylate block copolymers, or a combination thereof.
  • the dispersant may be styrene-acrylate polymer dispersant and may include hydrophilic monomers including acid monomers and hydrophobic monomers.
  • Hydrophobic monomers that can be polymerized in the acrylic dispersant may include for example, styrene, p-methyl styrene, methyl 30 methacrylate, hexyl acrylate, hexyl methacrylate, butyl acrylate, butyl methacrylate, ethyl acrylate, ethyl methacrylate, propyl acrylate, propyl methacrylate, octadecyl acrylate, octadecyl methacrylate, stearyl methacrylate, vinylbenzyl chloride, isobornyl acrylate, tetrahydrofurfuryl acrylate, 2-phenoxyethyl methacrylate, ethoxylated nonyl phenol methacrylate, iso
  • Acidic monomers that may be incorporated may include for example, acrylic acid, methacrylic acid, ethacrylic acid, dimethylacrylic acid, maleic anhydride, maleic acid, 10 vinylsulfonate, cyanoacrylic acid, vinylacetic acid, allylacetic acid, ethylidineacetic acid, propylideacetic acid, crotonoic acid, fumaric acid, itaconic acid, sorbic acid, angelic acid, cinnamic acid, styrylacrylic acid, citraconic acid, glutaconic acid, aconitic acid, phenylacrylic acid, acryloxypropionic acid, aconitic acid, phenylacrylic acid, acryloxypropionic acid, vinylbenzoic acid, N-vinylsucanamidic15 acid, mesaconic acid, methacroylatanine, acryloylhydroxyglycine, sulfoethyl methacrylic acid, s
  • a surface charge of the blocking particulates can have a zeta potential ranging from about 50 mV to about -50 mV, from about 25 mV to about25 -25 mV, from about 15 mV to about -15 mV, from about 50 mV to about 0 mV, or from about 0 mV to about -50 mV.
  • zeta potential refers to an electrokinetic potential of the blocking particulates in a colloidal dispersion. Zeta potential can be measured using a MastersizerTM 3000 particle analyzer, and may be measured as described in the user manual available from Malvern30 Panalytical.
  • the zeta potential can indicate a stability of a dispersion including the blocking particulates.
  • the stability of the dispersion will indicate whether or not the blocking particulates may flow through the porous substrate.
  • Blocking particulates with a zeta potential beyond the range of about 50 mV to about -50 mV may not flow through the porous substrate [0032]
  • the blocking particulates may be oriented to form a fluid barrier. 5
  • the blocking particulates may be positioned using a magnet and/or may be fused in a desired location on the porous substrate.
  • the fluid barrier may be in the shape of a fluidic routing barrier 232, a bed volume fluidic routing barrier 234, a fluidic mixing barrier 236, a fluid evaporation barrier 238, or a combination thereof, as illustrated in FIG.4. Also shown in FIG.4 is a self-wicking flow device10 200, a porous substrate 210, and a detecting region including a testing strip 214A and a control strip 214B.
  • a fluidic routing barrier may be used to limit an area of capillary flow through the porous substrate.
  • a bed volume fluidic routing barrier may be used to limit an area of capillary flow through the porous substrate such that the fluid may15 be directed to an area of interest such as the detecting region.
  • a bed volume fluidic routing barrier may be used to direct fluid flow to the detecting region to improve the sensitivity of the self-wicking flow device and may prevent a response in an area of the porous substrate which may not be visible.
  • Fluidic mixing barriers may be used to create mixing zones.
  • Fluidic evaporation barriers20 may be used to minimize sample evaporation from the porous substrate before the sample reaches the detecting zone.
  • the fluidic barrier may be located along a z-plane of the porous substrate.
  • the self-wicking flow device may include additional components.
  • the self-wicking flow device may include a support in the form of a25 housing, a backing card, or a combination thereof.
  • a housing can be a casing that the porous substrate may be disposed within.
  • the self-wicking flow device may further include a fluid inlet or port which may permit access to the porous substrate at or upstream of the complexing region.
  • the housing may further30 include a viewing window over the detecting region.
  • the viewing window may be an opening in the housing or may include an optically transparent material in the area of the detecting region to allow a user to view the results of a self-wicking, lateral or directional flow immunoassay test.
  • the self-wicking immunoassay device can further include a backing. The backing can support the porous substrate.
  • the backing can include polyphenylene 5 ether, polyester, polytetrafluoroethylene, glass, glass fiber, cellulose, nitrocellulose, or a combination thereof.
  • the backing can be used to provide stability to the porous substrate.
  • Self-Wicking Systems 10 [0036] Turning now to certain self-wicking systems, FIG.5 in particular depicts a self-wicking immunoassay system 300.
  • the self-wicking immunoassay system can include a lateral or directional flow device 200 that is self-wicking, and can include a fluid flow channel with a porous substrate 210 and blocking particulates.
  • the blocking particulates can exhibit magnetic properties and can be15 repositionable in response to a magnetic field to form a fluid barrier 230 to modulate fluid flow.
  • the blocking particulates can have an average particle size smaller than an average pore size of pores of the porous substrate.
  • the system can further include a magnet 310 associated with the self-wicking flow device to generate a magnetic field to reposition the blocking particulates within the20 self-wicking flow device.
  • the self-wicking flow device and components thereof may be as described above.
  • the magnet can be configured to generate a magnetic field that can be used to position, reposition, or hold the blocking particulates in a desired location.
  • the magnet can be used to generate a magnetic field that may hold the25 blocking particulates in any Cartesian coordinate (x, y, and/or z) within the porous substrate.
  • the magnet can be sized and shaped to generate a magnetic field in a desired location for formation of the fluid barrier.
  • the magnet can be configured to control a strength of the magnetic field generated.
  • the magnet may be electrically actuated.
  • the30 magnet can be embedded within or attached to a support for the self-wicking flow device, such as the housing or the backing card.
  • the magnet can be separate of the self-wicking flow device.
  • Aqueous Liquid Vehicles for Blocking Particulate Dispersions 5 may include water alone or in combination with a variety of additional components.
  • components that may be included, in addition to water may include co-solvent, surfactant, buffer, antimicrobial agent, anti-kogation agent, chelating agent, buffer, etc., which are commonly used in fluid agents that are ejected using ink-jet 10 architecture.
  • the aqueous liquid vehicle can include water and a co-solvent.
  • the aqueous liquid vehicle can include water, co-solvent, and a surfactant.
  • the aqueous liquid vehicle can include water, co-solvent, surfactant, and buffer or buffer and a chelating agent.
  • the aqueous liquid vehicle may include water as the primary solvent. The water may be deionized.
  • water can be present in the liquid vehicle at from about 50 wt% to 100 wt%, from about 50 wt% to about 93 wt%, from about 50 wt% to about 70 wt%, from about 90 wt% to 100 wt%, from about 75 wt% to about 95 wt%, or from about 55 wt% to about 65 wt%.
  • Examples of co-solvent(s) that may be present in the aqueous liquid vehicle can include ethanol, methanol, propanol, acetone, tetrahydrofuran, hexane, 1-butanol, 2-butanol, tert-butanol, isopropanol, propylene glycol, methyl ethyl ketone, dimethylformamide, 1,4-dioxone, acetonitrile, 1,2-butanediol, 1-methyl-2,3-propanediol, 2-pyrrolidone, glycerol, 2-phyenoxyethanol, 25 2-phenylethanol, 3-phenylpropanol, or a combination thereof.
  • a total amount of co-solvent(s) can range from about 2 wt% to about 50 wt%, from about 2 wt% to about 15 wt%, from about 10 wt% to about 25 wt%, from about 25 wt%, to about 50 wt%, or from about 5 wt% to about 12 wt%, based on a total weight of30 the aqueous liquid vehicle.
  • the aqueous liquid vehicle may also include surfactant.
  • the surfactant can include a non-ionic surfactant, a cationic surfactant, and/or an anionic surfactant.
  • Example non-ionic surfactants that can be used include self-emulsifiable, nonionic wetting agents based on acetylenic 5 diol chemistry (e.g., SURFYNOL ® SEF from Air Products and Chemicals, Inc., USA), a fluorosurfactant (e.g., CAPSTONE ® fluorosurfactants from DuPont, USA), or a combination thereof.
  • acetylenic 5 diol chemistry e.g., SURFYNOL ® SEF from Air Products and Chemicals, Inc., USA
  • a fluorosurfactant e.g., CAPSTONE ® fluorosurfactants from DuPont, USA
  • the surfactant can be an ethoxylated low-foam wetting agent (e.g., SURFYNOL ® 440, SURFYNOL ® 465, or SURFYNOL ® CT-111 from Air Products and Chemical Inc., USA) or an10 ethoxylated wetting agent and molecular defoamer (e.g., SURFYNOL ® 420 from Air Products and Chemical Inc., USA).
  • an ethoxylated low-foam wetting agent e.g., SURFYNOL ® 440, SURFYNOL ® 465, or SURFYNOL ® CT-111 from Air Products and Chemical Inc., USA
  • an10 ethoxylated wetting agent and molecular defoamer e.g., SURFYNOL ® 420 from Air Products and Chemical Inc., USA.
  • Still other surfactants can include wetting agents and molecular defoamers (e.g., SURFYNOL ® 104E from Air Products and Chemical Inc., USA), alkylphenylethoxylates, solvent-free surfactant blends (e.g., SURFYNOL ® CT-211 from Air Products and Chemicals, Inc., USA), 15 water-soluble surfactant (e.g., TERGITOL ® TMN-6, TERGITOL ® 15S7, and TERGITOL ® 15S9 from The Dow Chemical Company, USA), or a combination thereof.
  • wetting agents and molecular defoamers e.g., SURFYNOL ® 104E from Air Products and Chemical Inc., USA
  • alkylphenylethoxylates e.g., SURFYNOL ® CT-211 from Air Products and Chemicals, Inc., USA
  • 15 water-soluble surfactant e.g., TERGIT
  • the surfactant can include a non-ionic organic surfactant (e.g., TEGO ® Wet 510 from Evonik Industries AG, Germany), a non-ionic secondary alcohol ethoxylate (e.g., TERGITOL® 15-S-5, TERGITOL ® 20 15-S-7, TERGITOL ® 15-S-9, and TERGITOL ® 15-S-30 all from Dow Chemical Company, USA), or a combination thereof.
  • a non-ionic organic surfactant e.g., TEGO ® Wet 510 from Evonik Industries AG, Germany
  • a non-ionic secondary alcohol ethoxylate e.g., TERGITOL® 15-S-5, TERGITOL ® 20 15-S-7, TERGITOL ® 15-S-9, and TERGITOL ® 15-S-30 all from Dow Chemical Company, USA
  • Example anionic surfactants can include alkyldiphenyloxide disulfonate (e.g., DOWFAX ® 8390 and DOWFAX ® 2A1 from The Dow Chemical Company, USA), and oleth-3 phosphate surfactant (e.g., CRODAFOSTM N3 Acid from Croda, UK).
  • Example cationic surfactant that25 can be used can include dodecyltrimethylammonium chloride, hexadecyldimethylammonium chloride, or a combination thereof.
  • the surfactant (which may be a blend of multiple surfactants) may be present at an amount ranging from about 0.01 wt% to about 2 wt%, from about 0.05 wt% to about 1.5 wt%, or from about 0.01 wt% to about 1 wt%.
  • the aqueous liquid vehicle may further include a chelating agent, an antimicrobial agent, a buffer, or a combination thereof.
  • the aqueous liquid vehicle may include a chelating agent.
  • Chelating agent(s) can be used to minimize or to eliminate the deleterious effects of heavy metal impurities.
  • chelating agents can include disodium ethylene-diaminetetraacetic acid (EDTA-Na), ethylene diamine tetra acetic acid (EDTA), and methyl-glycinediacetic acid (e.g., TRILON ® M from BASF Corp., Germany). If included, whether a single chelating agent is used or a10 combination of chelating agents are used, the total amount of chelating agent(s) may range from about 0.01 wt% to about 2 wt% or from about 0.01 wt% to about 0.5 wt%.
  • the aqueous liquid vehicle may also include antimicrobial agents. Antimicrobial agents can include biocides and fungicides.
  • Example antimicrobial15 agents can include the NUOSEPT ® (Ashland Inc., USA), VANCIDE ® (R.T. Vanderbilt Co., USA), ACTICIDE ® B20 and ACTICIDE ® M20 (Thor Chemicals, U.K.), PROXEL ® GXL (Arch Chemicals, Inc., USA), BARDAC ® 2250, 2280, BARQUAT ® 50-65B, and CARBOQUAT ® 250-T, (Lonza Ltd. Corp., Switzerland), KORDEK® MLX (The Dow Chemical Co., USA), and combinations thereof.
  • a total amount of antimicrobial agents can range from about 0.01 wt% to about 1 wt%.
  • an aqueous liquid vehicle may further include a buffer.
  • the buffer can withstand small changes (e.g., less than 1) in pH when small quantities of a water-soluble acid or a water-soluble base are added to a25 composition containing the buffer.
  • the buffer can include a poly-hydroxy functional amine.
  • the buffer can include potassium hydroxide, 2-[4-(2-hydroxyethyl) piperazin-1-yl] ethane sulfonic acid, 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIZMA ® sold by Sigma-Aldrich, USA), 3-morpholinopropanesulfonic acid, triethanolamine, 30 2-[bis-(2-hydroxyethyl)-amino]-2-hydroxymethyl propane-1,3-diol (bis tris methane), N-methyl-D-glucamine, N,N,N’N’-tetrakis-(2-hydroxyethyl)- ethylenediamine and N,N,N’N’-tetrakis-(2-hydroxypropyl)-ethylenediamine, beta-alanine, betaine, or mixtures thereof.
  • potassium hydroxide 2-[4-(2-hydroxyethyl) piperazin-1-yl] ethane sulfonic acid
  • the buffer can include 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIZMA ® sold by Sigma-Aldrich, USA), beta-alanine, betaine, or mixtures thereof.
  • TEZMA 2-amino-2-(hydroxymethyl)-1,3-propanediol
  • beta-alanine betaine
  • the buffer can include 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIZMA ® sold by Sigma-Aldrich, USA), beta-alanine, betaine, or mixtures thereof.
  • numeric range that ranges from about 10 to about 500 should be interpreted to include the explicitly recited sub-range of about 10 to about 500 as well as25 sub-ranges thereof such as about 50 and about 300, as well as sub-ranges such as from about 100 to about 400, from about 150 to about 450, from about 25 to about 250, etc.

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Abstract

La présente divulgation concerne un procédé de fabrication d'une barrière de fluide pour un dispositif d'écoulement à effet de mèche automatique. Le procédé peut comprendre l'association de particules de blocage ayant des propriétés magnétiques avec un substrat poreux du dispositif d'écoulement à effet de mèche automatique. Les particules de blocage peuvent avoir une taille de particule moyenne inférieure à une taille de pore moyenne des pores du substrat poreux. Le procédé peut en outre comprendre le repositionnement des particules de blocage associées au substrat poreux à l'aide d'un aimant pour amener la particule de blocage à moduler l'écoulement de fluide.
PCT/US2020/055211 2020-10-12 2020-10-12 Dispositifs d'écoulement à effet de mèche automatique WO2022081124A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116679043A (zh) * 2023-08-03 2023-09-01 天津大学 适用于磁泳分析平台的机器人及制备方法、磁泳分析平台

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050175A1 (fr) * 1999-02-23 2000-08-31 Battelle Memorial Institute Appareil et procede de manipulation de particules magnetiques presentes dans un fluide
US20040166547A1 (en) * 2003-02-25 2004-08-26 Sullivan Brian M. Magnetic bead agglomerator for automated ELISA process
JP2007139649A (ja) * 2005-11-21 2007-06-07 Asahi Kasei Corp 分析装置の多孔体
KR101491921B1 (ko) * 2013-08-02 2015-02-12 고려대학교 산학협력단 시료 검출 장치
US20180100854A1 (en) * 2014-03-07 2018-04-12 The Regents Of The University Of California Methods and devices for integrating analyte extraction, concentration and detection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000050175A1 (fr) * 1999-02-23 2000-08-31 Battelle Memorial Institute Appareil et procede de manipulation de particules magnetiques presentes dans un fluide
US20040166547A1 (en) * 2003-02-25 2004-08-26 Sullivan Brian M. Magnetic bead agglomerator for automated ELISA process
JP2007139649A (ja) * 2005-11-21 2007-06-07 Asahi Kasei Corp 分析装置の多孔体
KR101491921B1 (ko) * 2013-08-02 2015-02-12 고려대학교 산학협력단 시료 검출 장치
US20180100854A1 (en) * 2014-03-07 2018-04-12 The Regents Of The University Of California Methods and devices for integrating analyte extraction, concentration and detection

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116679043A (zh) * 2023-08-03 2023-09-01 天津大学 适用于磁泳分析平台的机器人及制备方法、磁泳分析平台
CN116679043B (zh) * 2023-08-03 2023-11-03 天津大学 适用于磁泳分析平台的机器人及制备方法、磁泳分析平台

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