WO2022073332A1 - 一种恒温全自动化细胞制片一体化装置及制片方法 - Google Patents

一种恒温全自动化细胞制片一体化装置及制片方法 Download PDF

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Publication number
WO2022073332A1
WO2022073332A1 PCT/CN2021/088274 CN2021088274W WO2022073332A1 WO 2022073332 A1 WO2022073332 A1 WO 2022073332A1 CN 2021088274 W CN2021088274 W CN 2021088274W WO 2022073332 A1 WO2022073332 A1 WO 2022073332A1
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WIPO (PCT)
Prior art keywords
slide
settling
integrated device
cleaning
sample
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PCT/CN2021/088274
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English (en)
French (fr)
Inventor
陈石磊
朱海龟
汪钰青
聂垒
胡付余
王扩业
李一鸣
曾斌
陆龙飞
Original Assignee
嘉兴晶铸生物科技有限公司
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Application filed by 嘉兴晶铸生物科技有限公司 filed Critical 嘉兴晶铸生物科技有限公司
Priority to US18/029,678 priority Critical patent/US20230358647A1/en
Priority to EP21876855.4A priority patent/EP4206641A4/en
Publication of WO2022073332A1 publication Critical patent/WO2022073332A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00039—Transport arrangements specific to flat sample substrates, e.g. pusher blade
    • G01N2035/00049—Transport arrangements specific to flat sample substrates, e.g. pusher blade for loading/unloading a carousel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00089—Magazines
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00138—Slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0403—Sample carriers with closing or sealing means
    • G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0437—Cleaning cuvettes or reaction vessels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0439—Rotary sample carriers, i.e. carousels
    • G01N2035/0441—Rotary sample carriers, i.e. carousels for samples

Definitions

  • the invention relates to the technical field of instruments for pathological cells, diagnostics, and pathological biomolecular diagnostics, in particular to a cell sheet device, in particular to an automatic liquid-based thin-layer cell preparation device and a preparation method.
  • one of the objectives of the present invention is to provide an integrated device for constant temperature and fully automated cell preparation and a method for using the same.
  • the present invention proposes a constant temperature fully automated cell preparation integrated device, which includes:
  • a rotary settling pan the rotary settling pan includes a settling pan base and a turntable disc body connected to the settling pan body through a fixed shaft, wherein the bottom of the settling pan base is provided with a temperature control component, and the side wall of the settling pan base is A glass slide inlet, a glass slide outlet and a cleaning and sample addition inlet are arranged in sequence; at least one or more sedimentation cup accommodating grooves are evenly arranged along the circumferential edge of the turntable disc body to accommodate the settling cups; the turntable disc body intermittently At least four glass slide slots are formed between the turntable body and the rotary sinker base, and the glass slides to be added or the sampled glass slides are appropriately accommodated in the glass slides. in the card slot;
  • the slide loading mechanism is set at a position close to the glass slide inlet of the rotating sinker, and the slide loading mechanism pushes the slide to be loaded into the rotating sinker from the slide entrance position;
  • An integrated device for cleaning and adding samples is arranged on the side of the rotary sedimentation plate, and the integrated device for cleaning and adding liquid includes a cleaning liquid component and a sample adding component, and the cleaning liquid component and the sample adding component are accompanied by the turntable
  • the intermittent rotation of the disc body simultaneously adds liquid to one or more different settling cups;
  • a slide-out mechanism the slide-out mechanism is set at a position close to the glass slide outlet of the rotating sinker, and the slide-out mechanism moves the loaded glass slides out from the slide outlet position.
  • the outer wall and bottom of the settling pan base are provided with a thermal insulation layer
  • an inner layer of the thermal insulation layer close to the turntable body is provided with thermal insulation cotton
  • an outer layer away from the turntable body is provided with a metal cladding.
  • the temperature control assembly includes a temperature control element and a heating device connected to the temperature control element, the heating device is arranged at a position of the settling pan base corresponding to the settling cup accommodating groove, and the temperature control element comprises a resistance Regulator.
  • the temperature control component controls the temperature of the settling cup in the settling cup accommodating tank to be 20-60°C.
  • the slide loading mechanism comprises a slide cassette with an opening and a pushing device, the pushing device pushes the slides stored in the slide cassette to the slide inlet position through the opening, and is pushed by the glass slide.
  • the slide inlet goes into the slide card slot of the rotating sinker.
  • the integrated device for cleaning and adding samples further comprises a mechanical arm, one end of the mechanical arm is connected to the Z-direction adjustment mechanism, and the other end of the cleaning liquid component is fluidly connected to the sample adding component;
  • the Z-direction adjustment mechanism reciprocates in the vertical direction to adjust the relative height of the mechanical arm relative to the turntable body.
  • the robotic arm has an arcuate portion.
  • the film ejection mechanism includes a film ejection fork and a driving mechanism connected with the film ejection fork.
  • the settling cup accommodating groove and the glass slide clamping groove are arranged at intervals, and when the turntable disc body rotates intermittently, the slide clamping groove and the settling cup at the position of the slide inlet, the slide outlet and the cleaning and sample inlet position are accommodated in the settling cup.
  • the spaces between the slots are connected.
  • the present invention also proposes a method for using the above-mentioned constant temperature fully automated cell preparation integrated device, comprising the following steps:
  • Step S1 the glass slide to be loaded enters the rotary sinker from the slide inlet, and rotates intermittently with the turntable body;
  • Step S2 cleaning the cleaning liquid component and the sample adding component in the integrated sample adding device along with the intermittent rotation of the turntable disc body and adding liquid to one or more different settling cups respectively.
  • the loading glass slides are loaded with samples, and the cleaning component is used to clean the sedimentation cup that has been loaded with samples, and the loaded slides that have been loaded with samples are removed from the slide outlet.
  • the constant temperature fully automated cell preparation integrated device and its using method of the present invention have the following advantages and beneficial effects:
  • the settling cup By using the original settling cup, equipped with cleaning mechanism and cleaning reagents, the settling cup can be used multiple times, avoiding repeated filling of the settling cup to affect the efficiency, realizing automation, saving consumables and reducing costs.
  • the sedimentation cup can be cleaned at the same time when the Pasteur staining is performed, the mechanical structure is optimized, the sample waiting time is reduced, and the production efficiency is greatly improved.
  • Fig. 1 is the thermostatic fully automated cell preparation integrated device of the present invention
  • FIG. 2 is a schematic diagram of the exploded structure of the rotary sedimentation plate in the thermostatically fully automated cell preparation integrated device described in FIG. 1 .
  • FIG. 3 schematically shows the structure of the rotating sedimentation plate in some embodiments of the thermostatically-automated cell preparation integrated device according to the present invention from another perspective;
  • Fig. 4 illustrates the sample adding operation to the rotary sedimentation plate in the thermostatic fully automated cell preparation integrated device of the present invention
  • Fig. 5 illustrates the cleaning operation of the rotary sedimentation plate in the constant temperature fully automated cell preparation integrated device of the present invention
  • Fig. 6 is the fork structure in the constant temperature fully automated cell preparation integrated device according to the present invention.
  • Fig. 7 schematically illustrates the pusher mechanism in the thermostatic fully automated cell preparation integrated device of the present invention
  • FIG. 8 illustrates the fork operation of the slide pushing device in the thermostatic fully automated cell preparation integrated device according to the present invention to perform slide production.
  • Fig. 1 is the thermostatic fully automated cell preparation integrated device according to the present invention.
  • the automatic liquid-based thin-layer cell preparation device includes a rotary sedimentation plate 12, a slide storage and push mechanism 25, a sample needle 01, and a pipetting mechanism 26.
  • the slide storage and push mechanism 25 includes a slide cassette 02 , a slide-in fork 08 , and a slide-out fork 13 .
  • the pipetting mechanism 26 includes a reagent arm Z axis 10 , a lateral reagent arm R axis 09 , and a reagent needle assembly 27 located at the end of the lateral reagent arm R axis 09 .
  • the reagent needle assembly 27 forms an arc and is located just above the sedimentation cup 05 .
  • the sedimentation tablet assembly 30 includes a glass slide slot 23 , a jacking mechanism 07 , and a sedimentation cup 05 .
  • the jacking mechanism 07 controls the sedimentation cup 05 to move up and down along the central axis of the constant temperature turntable 17 .
  • the jacking mechanism 07 controls the settling cup 05 to lift up, the shifting fork motor 18 drives the slide fork 08 to push the slides in the slide cassette 02 into the slide slot 23 from the slide inlet 24, and the jacking mechanism 07 controls the settling cup 05 Descend to seal the bottom of the sinker cup 05 with the glass slide.
  • the reagent needle assembly 27 includes a vertically arranged arc-shaped support 28, an integrated reagent needle 04, and an integrated cleaning needle 03.
  • the integrated reagent needle 04 and the integrated cleaning needle 03 are connected to an external container through an internal suction pipe.
  • the lift drive motor 19 drives the reagent arm Z axis 10 to move up and down to complete reagent filling and waste liquid extraction.
  • the cleaning assembly 29 includes a cleaning plate and a cleaning plate pushing mechanism 11 .
  • the thermostatic turntable 17 rotates at least once every time the pasteurization or sedimentation cup cleaning is completed, and the next sedimentation cup 05 to be operated is transferred to the bottom of the reagent needle assembly 27 .
  • the integrated reagent needle 04 cooperates with the integrated cleaning needle 03, and when the sample in one sedimentation cup is pasteurized, the other sedimentation cup in a washable state can be cleaned.
  • the temperature control system includes a heating belt 14 and a temperature control assembly 15 .
  • the temperature is controllably adjusted in the range of 20°C to 45°C, and a stable external environment is provided for the dyeing reaction through the outer thermal insulation shell 16 .
  • the slide cassette 02 loaded with the slides is fastened with the slide cassette buckle 22 and inserted into the slide cassette catch slot 21 to complete the fixing.
  • the jack-up mechanism 07 controls the sinker cup 05 to rise, the slide-in fork 08 pushes out the glass slide, and enters the corresponding slide slot 23 from the slide inlet 24 through the rotation of the rotary sinker 12 .
  • the lifting mechanism 07 puts down the sedimentation cup 05, and the bottom of the sedimentation cup 05 is closed with the glass slide.
  • the sample adding needle 01 injects the sample into the sedimentation cup 05, the thermostatic turntable moves the sedimentation cup to the lower part of the integrated reagent needle, and the elevating drive motor 19 drives the reagent arm Z-axis 10 to descend to perform the Pasteur staining process.
  • the slide slot is rotated to the slide-out position, the slide-out fork 13 descends first, and enters the thermostatic turntable from the slide outlet 32; , and pull the slide out.
  • the slide card slot rotates to the cleaning position, the jacking mechanism controls the sedimentation cup to rise, the cleaning plate motor 20 drives the cleaning plate pushing mechanism 11 to push the cleaning plate from the cleaning plate inlet 33 into the glass card slot 23, and the jacking mechanism controls the sedimentation cup 05
  • the bottom of the sedimentation cup 05 is closed with the cleaning plate 31 to form a closed structure, and the integrated cleaning needle 03 passes the cleaning reagent into the sedimentation cup 05.
  • the integrated cleaning needle 03 draws out the waste liquid, and the jacking mechanism controls the sedimentation cup to rise and clean.
  • the plate motor 20 drives the cleaning plate pushing mechanism to pull out the cleaning plate, and the jacking mechanism controls the descending of the settling cup.
  • the reagent and dye solution are transferred through the integrated reagent needle, the cleaning solution of the sedimentation cup is transferred through the integrated cleaning needle, and the slides contain cationic high-adsorption slides.
  • the automatic liquid-based thin-layer cell preparation device includes a rotary sedimentation plate 12, a slide storage and push mechanism 25, a sample needle 01, a liquid pipetting mechanism 26, and a cleaning component 29; wherein: the sedimentation plate 12 includes a number of peripherally arranged The thermostatic turntable 17 of the spacer glass slot 23 , the thermal insulation shell 16 , and the sedimentation tablet assembly 30 .
  • the slide storage and push mechanism 25 includes a slide cassette 02, a slide-in fork 08, and a slide-out fork 13.
  • the pipetting mechanism 26 includes a reagent arm Z axis 10 , a lateral reagent arm R axis 09 , and a reagent needle assembly 27 located at the end of the lateral reagent arm R axis 09 .
  • the reagent needle assembly 27 forms an arc and is located just above the sedimentation cup 05 .
  • the sedimentation tablet assembly 30 includes a glass slide slot 23 , a jacking mechanism 07 , and a sedimentation cup 05 .
  • the jacking mechanism 07 controls the sedimentation cup 05 to move up and down along the central axis of the constant temperature turntable 17 .
  • the jacking mechanism 07 controls the settling cup 05 to lift up, the shifting fork motor 18 drives the slide fork 08 to push the slides in the slide cassette 02 from the slide inlet 24 into the slide slot 23, and the jacking mechanism 07 controls the settling cup 05 Descend to seal the bottom of the sinker cup 05 with the glass slide.
  • the reagent needle assembly 27 includes a vertically arranged arc-shaped support 28, an integrated reagent needle 04, and an integrated cleaning needle 03.
  • the integrated reagent needle 04 and the integrated cleaning needle 03 are connected to an external container through an internal suction pipe.
  • the lift drive motor 19 drives the reagent arm Z axis 10 to move up and down to complete reagent filling and waste liquid extraction.
  • the cleaning assembly 29 includes a cleaning plate and a cleaning plate pushing mechanism 11 .
  • the thermostatic turntable 17 rotates at least once every time the pasteurization or sedimentation cup cleaning is completed, and the next sedimentation cup 05 to be operated is transferred to the bottom of the reagent needle assembly 27 .
  • the integrated reagent needle 04 cooperates with the integrated cleaning needle 03, and when the sample in one sedimentation cup is pasteurized, the other sedimentation cup in a washable state can be cleaned.
  • the temperature control system includes a heating belt 14 and a temperature control assembly 15 .
  • the temperature is controllably adjusted in the range of 20°C to 45°C, and a stable external environment is provided for the dyeing reaction through the outer thermal insulation shell 16 .
  • the slide cassette 02 loaded with the slides is fastened with the slide cassette buckle 22 and inserted into the slide cassette catch slot 21 to complete the fixing.
  • the jack-up mechanism 07 controls the sinker cup 05 to rise, the slide-in fork 08 pushes out the glass slide, and enters the corresponding slide slot 23 from the slide inlet 24 through the rotation of the rotary sinker 12 .
  • the lifting mechanism 07 puts down the sedimentation cup 05, and the bottom of the sedimentation cup 05 is closed with the glass slide.
  • the sample adding needle 01 injects the sample into the sedimentation cup 05, the thermostatic turntable moves the sedimentation cup to the lower part of the integrated reagent needle, and the elevating drive motor 19 drives the reagent arm Z-axis 10 to descend to perform the Pasteur staining process.
  • the slide slot is rotated to the slide-out position, the slide-out fork 13 descends first, and enters the thermostatic turntable from the slide outlet 32; , and pull the slide out.
  • the slide card slot rotates to the cleaning position, the jacking mechanism controls the sedimentation cup to rise, the cleaning plate motor 20 drives the cleaning plate pushing mechanism 11 to push the cleaning plate from the cleaning plate inlet 33 into the glass card slot 23, and the jacking mechanism controls the sedimentation cup 05
  • the bottom of the sedimentation cup 05 is closed with the cleaning plate 31 to form a closed structure, and the integrated cleaning needle 03 passes the cleaning reagent into the sedimentation cup 05.
  • the integrated cleaning needle 03 draws out the waste liquid, and the jacking mechanism controls the sedimentation cup to rise and clean.
  • the plate motor 20 drives the cleaning plate pushing mechanism to pull out the cleaning plate, and the jacking mechanism controls the descending of the settling cup.
  • the glass slides are automatically loaded into the machine and tightly joined with the sedimentation cup;
  • the processed sample is added to the sedimentation cup through the sample injection needle;
  • the temperature of the constant temperature static settling is controlled between 20°C and 60°C.
  • the operation steps are as follows: the sample is left to settle for 3-15 minutes, the supernatant is removed, 0.5-2ml of absolute ethanol or isopropanol is added, and 8-12 seconds later, the sample is removed; then, add 0.5-2ml of pH7.7tris buffer, sucked away after 3-8 seconds; added 0.5-2ml of hematoxylin staining solution, sucked away after 1-5 minutes; added 0.5-2ml of pH7.7tris buffer, sucked away after 0.5-1 minute ; Add 0.5-2ml of absolute ethanol or isopropanol, suck it off after 3-8 seconds; add 0.5-2ml of absolute ethanol or isopropanol, suck it off after 3-8 seconds; add EA-OG mixed dye solution 0.5- 2ml, sucked away after 1-5 minutes; add 0.5-2ml of absolute ethanol or isopropanol, sucked away after 5-10 seconds; add 0.5-2ml of absolute ethanol or iso
  • the constant temperature and fully automated cell preparation integrated device further includes a sealing device, which is used for sealing the glass slide after the glass slide is transferred out of the slide card slot, and the sealing device is used for sealing the glass slide.
  • the film device includes a frame, a shifting fork, a glue application arm, a sealing glue reagent needle, a curing cup, a curing box, an ultraviolet lamp, a first control motor 7 and a second control motor.
  • the rack is provided with a sample slide rail
  • the shift fork is arranged at one end of the sample slide rail, and can slide in the sample slide rail, and the upper part of the sample slide rail is sequentially arranged from the side where the shift fork is provided to the other side
  • a curing box and an ultraviolet lamp a curing cup is arranged on the curing box, a glue adding arm is arranged above the curing cup, and a sealing glue reagent needle is arranged on the glue arm; the first control motor and the second control motor are arranged on the machine.
  • the first control motor is connected to the glue arm and can drive the glue arm to move in the vertical direction
  • the second control motor is connected to the glue arm and can drive the glue arm to move in the horizontal direction
  • the slide is placed on the sample slide rail It can slide in the sample slide rail under the action of the fork.
  • the first control motor is connected to the gluing arm through a gear or belt transmission mechanism, and can drive the gluing arm to rotate in the horizontal direction;
  • the second control motor is connected to the gluing arm through a rack-and-pinion mechanism or a worm gear mechanism, and is capable of driving the gluing arm to rotate in the horizontal direction. It can drive the glue arm to move up and down.
  • the bottom of the curing box is provided with an accommodating groove, the accommodating groove can accommodate the slide glass, and a through hole is arranged in the middle of the curing box, and the through hole is the accommodating position of the curing cup; the through hole is communicated with the accommodating groove.
  • the bottom of the curing box is provided with a guide spring column; the bottom of the curing cup is hollow.
  • the curing cup accommodating position on the curing box is provided with a limit slot in the circumferential direction, and the curing cup is provided with a protruding clip in the circumferential direction, and the clip can be matched and connected with the limit slot.
  • the width of the accommodating groove at the bottom of the curing box matches the size of the sample slide.
  • the accommodating groove at the bottom of the curing box clamps the sample slide to prevent the sample slide from moving.
  • the size of the through-hole matches the size of the curing cup, and the clamping block arranged on the circumference of the curing cup is matched and connected with the limit slot on the curing box, thereby restricting the curing cup to be stably installed on the curing box, and will not move or move. It will rotate, so as not to affect the subsequent dispensing process, so that the dispensing position remains fixed.
  • the guide spring column at the bottom of the curing box is in a state of energy storage when the glue application arm presses the fastening cup on the surface of the sample slide.
  • the energy storage of the guide spring column will automatically lift the curing cup. This separates the curing cup from the sample slide.
  • the bottom of the curing cup is hollowed out in a circular shape, so that the sealing glue is fixed on the sample slide to form a circular curing layer.
  • a lampshade can be provided outside the ultraviolet lamp, the lampshade is located above the sample slide rail, and the sample slide after dispensing is cured by the ultraviolet lamp in the lampshade.
  • the glue adding arm is provided with a sealing glue accommodating device, an injection system, a suction system and a waste glue accommodating device, and the reagent needle includes a glue feeding needle and a glue suction needle;
  • the sealing glue accommodating device is connected to the injection system,
  • the injection system is connected to the glue adding needle, the glue suction needle is connected to the suction system, and the suction system is connected to the waste glue accommodating device.
  • the injection system is capable of dripping the sealing glue in the sealing glue accommodating device onto the surface of the sample slide through a glue application needle, and the volume of the glue injected by the injection system is measured by a metering device, and the volume of the glue drop is controlled by the metering device. ;
  • the suction system can suck the excess sealing glue to the waste glue accommodating device through the glue suction needle.
  • the constant temperature and fully automated cell preparation integrated device further includes a perforated plate filter device, which is used for positive pressure blowing on the glass slide.
  • the porous plate filtering device includes a porous plate, a clamping jaw, a moving component, a guide block, an air blowing positive pressure block and a frame; the moving component, the guiding block and the air blowing positive pressure block are all arranged on the frame, and the clamping jaw
  • the moving assembly is connected and can move under the action of the moving assembly.
  • the clamping jaw can grab the perforated plate, and the perforated plate is installed on the guide block, and the guide block is located under the blowing positive pressure block.
  • the moving assembly includes an X-axis slide rail, a Y-axis slide rail, the Y-axis slide rail is mounted on the X-axis slide rail and can move on the X-axis slide rail, and the gripper is mounted on the Y-axis slide rail and can slide on the Y-axis slide rail. Movement on the track. Also included is a stack assembly that includes a multi-layer placement location capable of placing a multi-well plate.
  • the guide block is movably connected to the frame, and the guide block can translate and rotate under the blowing positive pressure block.
  • the blowing positive pressure block is movably connected to the frame, and the blowing positive pressure block can translate and/or rotate above the guide block; the blowing positive pressure block can be connected to external air through a pipeline.
  • the perforated plate includes a grid, a filter membrane and a filtrate disc, the filter membrane is connected to the bottom of the well, the filtrate tray is detachably connected under the filter membrane, and both sides of the perforated plate are turned outward to form a clamping position.
  • One or more clamping strips are arranged at the bottom of the edge of the porous plate, and one or more clamping grooves are arranged on the clamping jaws. When the clamping jaws are clamped in the clamping position of the perforated plate, the clamping strips and the clamping grooves are matched one-to-one. connection; a cut corner is set on one side edge of the perforated plate to form a placement mark.
  • There are a plurality of the cells and the cells are arranged in multiple rows and columns; the cells are in the shape of a door arch, a T shape or a triangle.
  • the diameter of the filter pores of the filter membrane is 1-50 microns.
  • the perforated plate includes 24 door arch-shaped cells, arranged in 6 rows and 4 columns, the filter membrane adopts a screen mesh, and the screen mesh is welded to the bottom of the cell by a heat welding process, and the gate arch-shaped cell
  • the diameter of the arc part is ⁇ 4-50 mm
  • the width of the straight edge of the door arch cell is 4-50 mm
  • the depth of the door arch cell is 5-40 mm
  • the liquid filling volume of the perforated plate is 20-3000 microliters.
  • the board material is PES (polyethersulfone resin), PE (polyethylene) or PET (polyethylene terephthalate).

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Abstract

一种恒温全自动化细胞制片一体化装置，其包括：旋转沉降盘（12），旋转沉降盘（12）包括沉降盘盘座以及通过固定轴与沉降盘盘体连接的转盘盘体；设于靠近旋转沉降盘（12）的玻片入口（24）位置的加片机构，其将待加样玻片由玻片入口（24）位置推送入旋转沉降盘（12）内；清洗加样集成装置，清洗加样集成装置设于旋转沉降盘（12）旁侧，清洗加样集成装置包括清洗组件（29）以及加样组件，清洗组件（29）以及加样组件随同转盘盘体的间歇转动同时分别向不同的一个或多个沉降杯（05）内加液；设于靠近旋转沉降盘（12）的玻片出口（32）位置的出片机构，出片机构将已加样玻片由玻片出口（32）位置移出。

Description

一种恒温全自动化细胞制片一体化装置及制片方法 技术领域
本发明涉及病理细胞、诊断学、病理生物分子诊断学用仪器技术领域，具体涉及一种细胞片装置，特别涉及一种全自动液基薄层细胞制片装置及制片方法。
背景技术
在医学检测和生物学研究中，常需要通过对细胞样本进行制片与染色，以便更好地观察。传统的制片染色过程均采用手工操作，但是由于手工涂片存在着大量的细胞堆积以及红细胞、黏液、杂质等干扰因素而影响正确诊断，会出现2％～50％的假阴性率，并且容易使诊断人员眼工作疲劳，且费时费力，效率低等问题。近年来，液基细胞学技术的应用使细胞的制片与染色技术有了新的突破，液基细胞学技术是一种将脱落细胞保存在细胞保存液中，能有效祛除红细胞、黏液、杂质等干扰诊断因素并将细胞团分散离解，显著改变病理细胞学制片质量，细胞结构和背景清晰，利于鉴别诊断，明显降低了假阴性率。
通过对文献和专利的检索，与细胞制片相关的专利及文献，例如：公开号为CN106018033A，公开日为2016年10月12日，名称为“全自动液基薄层细胞制片染色装置”的中国专利文献公开了一种全自动液基薄层细胞制片染色装置。在该专利文献所公开的技术方案中，装置可以全自动实现放样，移液，制片，染色的机构。其虽然在一定程序上能够自动制片，部分取代人工操作，但是该制片染色装置将在不同的温度将会导致不同的染色深浅，缺少有效的质量控制，同时其仅能实现放样与染色，而不能实现清洗，收片等全流程的自动化，因此还是需要人工的参与，从而提高了人力成本与制片时间，不利于降低整个过程的成本。
发明内容
针对现有技术中的缺陷，本发明的目的之一在于提供一种恒温全自动化细胞制片一体化装置及其使用方法。
有鉴于此，本发明提出了一种恒温全自动化细胞制片一体化装置，其包括：
旋转沉降盘，所述旋转沉降盘包括沉降盘盘座以及通过固定轴与沉降盘盘体连接的 转盘盘体，其中，所述沉降盘盘座底部设有温控组件，沉降盘盘座侧壁依次排列设有玻片入口、玻片出口以及清洗加样入口；沿转盘盘体圆周边缘均匀设置有至少一个或多个沉降杯容置槽，以容置沉降杯；所述转盘盘体间歇性地绕所述固定轴转动，所述转盘盘体与旋转沉降盘盘座间形成至少四个玻片卡槽，待加样玻片或是已加样玻片适配地容置于所述玻片卡槽内；
加片机构，所述加片机构设于靠近所述旋转沉降盘的玻片入口位置，所述加片机构将待加样玻片由所述玻片入口位置推送入旋转沉降盘内；
清洗加样集成装置，所述清洁加液集成装置设于所述旋转沉降盘旁侧，所述清洗加液集成装置包括清洗液组件以及加样组件，所述清洗液组件以及加样组件随同转盘盘体的间歇转动同时分别向不同的一个或多个沉降杯内加液；
出片机构，所述出片机构设于靠近所述旋转沉降盘的玻片出口位置，所述出片机构将已加样玻片由所述玻片出口位置移出。
优选地，所述沉降盘盘座外壁及底部设有保温层，所述保温层接近所述转盘盘体的内层设有保温棉，而远离转盘盘体的外层设有金属包层。
优选地，所述温控组件包括温控元件以及与温控元件连接的加热装置，所述加热装置设于所述沉降盘盘座对应沉降杯容置槽的位置，所述温控元件包括电阻调节器。
优选地，所述温控组件控制所述沉降杯容置槽内的沉降杯温度为20～60℃。
优选地，所述加片机构包括开设有开口的玻片匣以及推送装置，所述推送装置将存储于玻片匣内的玻片经所述开口推送至玻片入口位置，并由所述玻片入口进入旋转沉降盘的玻片卡槽内。
优选地，所述清洗加样集成装置还包括机械臂，所述机械臂一端与Z向调节机构连接，另一端清洗液组件与加样组件的流体连接；
所述Z向调节机构沿竖直方向往复运动，以调节机械臂相对于转盘盘体的相对高度。
优选地，所述机械臂具有弧形部。
优选地，所述出片机构包括出片拨叉以及与出片拨叉连接的驱动机构。
优选地，所述沉降杯容置槽与玻片卡槽间隔设置，当转盘盘体间歇转动时，玻片入口、玻片出口以及清洗加样入口位置处的玻片卡槽与沉降杯容置槽间的空间连通。
相应的，本发明还提出了一种采用上述的恒温全自动化细胞制片一体化装置使用方法，包括如下步骤：
步骤S1：待加样玻片由玻片入口进入旋转沉降盘内，并随同转盘盘体间歇转动；
步骤S2：清洗加样集成装置中的清洗液组件以及加样组件随同转盘盘体的间歇转动同时分别向不同的一个或多个沉降杯内加液，此时，加样组件通过沉降杯向待加样玻片加样，而清洗组件则对已加完样的沉降杯进行清洗，已加完样的已加样玻片则由玻片出口移出。
与现有技术相比，本发明所述的恒温全自动化细胞制片一体化装置及其使用方法具有如下所述的优点以及有益效果：
⑴在不同的温度下，染料着色的深浅存在不同，从而导致制片效果的差异。通过采用温控系统，从而可以在不同的季节、地域条件下给染色反应提供稳定的外环境，获得稳定的制片效果。
⑵通过采用独创性的沉降杯，配以清洗机构和清洗试剂，这样可以实现沉降杯的多次使用，避免反复装填沉降杯影响效率，实现了自动化，节约耗材，降低成本。
⑶通过采用协同工作的集成试剂针和集成清洗针，这样可以在进行巴氏染色的时候，同时进行沉降杯的清洗，优化了机械结构，减少了样本等待时间，大大提高了制片效率。
⑷通过大容量的玻片匣以及自动推片结构，这样可以减少人工参与的次数，极大地减少了装填耗材的次数，提升了整个制片流程的自动化水平。
附图说明
通过阅读参照以下附图对非限制性实施例所作的详细描述，本发明的其它特征、目的和优点将会变得更明显：
图1为本发明所述的恒温全自动化细胞制片一体化装置；
图2为图1的述的恒温全自动化细胞制片一体化装置中的旋转沉降盘的分解结构示意图。
图3从另一视角示意性地显示了本发明所述的恒温全自动化细胞制片一体化装置在一些实施方式中的旋转沉降盘的结构；
图4示意了本发明所述的恒温全自动化细胞制片一体化装置中对旋转沉降盘的加样操作；
图5示意了本发明所述的恒温全自动化细胞制片一体化装置中对旋转沉降盘的清洗操作；
图6为本发明所述的恒温全自动化细胞制片一体化装置中的拨叉结构；
图7示意了本发明所述的恒温全自动化细胞制片一体化装置中的推片机构；
图8示意了本发明所述的恒温全自动化细胞制片一体化装置中推片装置进行出片的拨叉操作。
附图标记如下所示：
Figure PCTCN2021088274-appb-000001
具体实施方式
下面结合具体实施例对本发明进行详细说明。以下实施例将有助于本领域的技术人员进一步理解本发明，但不以任何形式限制本发明。应当指出的是，对本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变化和改进。这些都属于本发明的保护范围。
实施例1
图1为本发明所述的恒温全自动化细胞制片一体化装置。
如图1所示，并在必要时结合图2至图8，全自动液基薄层细胞制片装置，包括旋转沉降盘12、玻片存储与推送机构25、加样针01、移液机构26、清洗组件29；其中：沉降盘12包括外周向设置有若干间隔玻片卡槽23的恒温转盘17、保温外壳16、沉降制片组件30。其中：玻片存储与推送机构25包括玻片匣02、入片拨叉08、出片拨叉13。移液机构26包括试剂臂Z轴10、横向试剂臂R轴09、位于横向试剂臂R轴09末端的试剂针组件27。试剂针组件27形成弧形位于沉降杯05正上方。
进一步地，恒温转盘17内部集成有温控系统，保温外壳16将沉降盘12外周和下部包裹。沉降制片组件30包括玻片卡槽23、顶升机构07、沉降杯05，顶升机构07控制沉降杯05沿恒温转盘17的中心轴方向上下运动。
顶升机构07控制沉降杯05抬起，拨叉电机18驱动入片玻叉08将玻片匣02 内玻片从玻片入口24推入玻片卡槽23，顶升机构07控制沉降杯05下降，使沉降杯05底部与玻片密闭接合。
试剂针组件27包括竖向设置的弧形支架28、集成试剂针04、集成清洗针03，集成试剂针04和集成清洗针03通过内吸管道与外部容器连接。升降驱动电机19驱动试剂臂Z轴10上下运动，完成试剂加注和废液抽取。清洗组件29包括清洗板、清洗板推送机构11。
恒温转盘17每完成一次巴氏染色或者沉降杯清洗，恒温转盘17转动至少一次，将下一个需要操作的沉降杯05转移至试剂针组件27下方。
集成试剂针04与集成清洗针03协同工作，对一个沉降杯内样本进行巴氏染色时，可对另一个处于可清洗状态的沉降杯进行清洗。
更为具体地，温控系统包括加热带14和温控组件15。可控地调节温度在20℃-45℃区间，并通过外围的保温外壳16，为染色反应提供稳定的外环境。
进一步具体地，装好玻片的玻片匣02扣好玻片匣卡扣22，插入玻片匣卡槽21内，完成固定。顶升机构07控制沉降杯05上升，入片拨叉08将玻片推出，通过旋转沉降盘12的旋转，从玻片入口24进入相应的玻片卡槽23。顶升机构07将沉降杯05放下，沉降杯05底部与玻片闭合。加样针01将样本注入沉降杯05，恒温转盘将沉降杯转至集成试剂针下方，升降驱动电机19驱动试剂臂Z轴10下降，进行巴氏染色流程。巴氏染色完成后，玻片卡槽旋转至出片位置，出片拨叉13先下降，从玻片出口32进入恒温转盘，出片拨叉13上升至玻片下方，出片拨叉13退出，将玻片拉出。玻片卡槽旋转至清洗位，顶升机构控制沉降杯上升，清洗板电机20驱动清洗板推送机构11将清洗板由清洗板入口33推入玻片卡槽23，顶升机构控制沉降杯05下降，沉降杯05底部与清洗板31闭合形成密闭结构，集成清洗针03将清洗试剂通入沉降杯05，清洗完成后，集成清洗针03将废液抽出，顶升机构控制沉降杯上升，清洗板电机20驱动清洗板推送机构把清洗板拉出，顶升机构控制沉降杯下降。
试剂与染液通过集成试剂针转移，沉降杯清洗液通过集成清洗针转移，载玻片包含阳离子高吸附载玻片。
全自动液基薄层细胞制片装置，包括旋转沉降盘12、玻片存储与推送机构25、加样针01、移液机构26、清洗组件29；其中：沉降盘12包括外周向设置有若干间隔玻片卡槽23的恒温转盘17、保温外壳16、沉降制片组件30。其中：玻片存储与 推送机构25包括玻片匣02、入片拨叉08、出片拨叉13。移液机构26包括试剂臂Z轴10、横向试剂臂R轴09、位于横向试剂臂R轴09末端的试剂针组件27。试剂针组件27形成弧形位于沉降杯05正上方。
进一步地，恒温转盘17内部集成有温控系统，保温外壳16将沉降盘12外周和下部包裹。沉降制片组件30包括玻片卡槽23、顶升机构07、沉降杯05，顶升机构07控制沉降杯05沿恒温转盘17的中心轴方向上下运动。
顶升机构07控制沉降杯05抬起，拨叉电机18驱动入片玻叉08将玻片匣02内玻片从玻片入口24推入玻片卡槽23，顶升机构07控制沉降杯05下降，使沉降杯05底部与玻片密闭接合。
试剂针组件27包括竖向设置的弧形支架28、集成试剂针04、集成清洗针03，集成试剂针04和集成清洗针03通过内吸管道与外部容器连接。升降驱动电机19驱动试剂臂Z轴10上下运动，完成试剂加注和废液抽取。清洗组件29包括清洗板、清洗板推送机构11。
恒温转盘17每完成一次巴氏染色或者沉降杯清洗，恒温转盘17转动至少一次，将下一个需要操作的沉降杯05转移至试剂针组件27下方。
集成试剂针04与集成清洗针03协同工作，对一个沉降杯内样本进行巴氏染色时，可对另一个处于可清洗状态的沉降杯进行清洗。
更为具体地，温控系统包括加热带14和温控组件15。可控地调节温度在20℃-45℃区间，并通过外围的保温外壳16，为染色反应提供稳定的外环境。
进一步具体地，装好玻片的玻片匣02扣好玻片匣卡扣22，插入玻片匣卡槽21内，完成固定。顶升机构07控制沉降杯05上升，入片拨叉08将玻片推出，通过旋转沉降盘12的旋转，从玻片入口24进入相应的玻片卡槽23。顶升机构07将沉降杯05放下，沉降杯05底部与玻片闭合。加样针01将样本注入沉降杯05，恒温转盘将沉降杯转至集成试剂针下方，升降驱动电机19驱动试剂臂Z轴10下降，进行巴氏染色流程。巴氏染色完成后，玻片卡槽旋转至出片位置，出片拨叉13先下降，从玻片出口32进入恒温转盘，出片拨叉13上升至玻片下方，出片拨叉13退出，将玻片拉出。玻片卡槽旋转至清洗位，顶升机构控制沉降杯上升，清洗板电机20驱动清洗板推送机构11将清洗板由清洗板入口33推入玻片卡槽23，顶升机构控制沉降杯05下降，沉降杯05底部与清洗板31闭合形成密闭结构，集成清洗针03将清洗试剂通入沉降杯05，清洗完成后，集成清洗针03将废液抽出，顶升机构 控制沉降杯上升，清洗板电机20驱动清洗板推送机构把清洗板拉出，顶升机构控制沉降杯下降。
上述过程中，本发明所述的恒温全自动化细胞制片装置的操作流程如下所述：
玻片自动上机，与沉降杯密闭接合；
将处理后的样本加入沉降杯，恒温沉降；
对沉降细胞进行巴氏染色；
将巴氏染色后的玻片转移出玻片卡槽，对沉降杯进行清洗；
处理后的样本通过加样针加入沉降杯；
其中，恒温静置沉降的温度控制在20℃-60℃之间。
进行巴氏染色时，其操作步骤如下所述：样本静置沉降3-15分钟，吸去上清后加入无水乙醇或异丙醇0.5-2ml，8-12秒后吸走；随后，加入PH7.7tris缓冲液0.5-2ml，3-8秒后吸走；加入苏木素染液0.5-2ml，1-5分钟后吸走；加入PH7.7tris缓冲液0.5-2ml，0.5-1分钟后吸走；加入无水乙醇或异丙醇0.5-2ml，3-8秒后吸走；加入无水乙醇或异丙醇0.5-2ml，3-8秒后吸走；加入EA-OG混合染液0.5-2ml，1-5分钟后吸走；加入无水乙醇或异丙醇0.5-2ml，5-10秒后吸走；加入无水乙醇或异丙醇0.5-2ml，5-10秒后吸走；出片拨叉将玻片转移出玻片卡槽后，将清洗板移动到玻片卡槽，对沉降杯进行清洗。
实施例2
在本实施例中的恒温全自动化细胞制片一体化装置还包括封片装置，所述封片装置用于在玻片转移出玻片卡槽后，对该玻片进行封片，所述封片装置包括机架、拨叉、加胶臂、封片胶试剂针、固化杯、固化盒、紫外灯、第一控制电机7以及第二控制电机。
其中，所述机架上设置有样本滑轨，拨叉设置在样本滑轨的一端，并能够在样本滑轨内滑动，样本滑轨的上方从设置拨叉的一侧到另一侧依次设置有固化盒、紫外灯，固化盒上设置有固化杯，固化杯上方设置有加胶臂，加胶臂上设置有封片胶试剂针；所述第一控制电机、第二控制电机设置在机架上，第一控制电机连接加胶臂并能够驱动加胶臂在竖直方向运动，第二控制电机连接加胶臂并能够驱动加胶臂在水平方向运动；载玻片放置在样本滑轨内并能够在拨叉的作用下在样本滑轨内滑动。
此外，第一控制电机通过齿轮或皮带传输机构连接加胶臂，并能够驱动加胶臂在水平方向内转动；所述第二控制电机通过齿轮齿条机构或者涡轮蜗杆机构连接加胶臂，并能够驱动加胶臂上下运动。
固化盒底部设置有容纳槽，所述容纳槽能够放置载玻片，固化盒中间设置有一个贯穿孔，所述贯穿孔为固化杯容纳位置；所述贯穿孔与容纳槽连通。所述固化盒底部设置有导向弹簧柱；所述固化杯底部镂空。所述固化盒上的固化杯容纳位置周向上设置有限位卡槽，所述固化杯的周向上设置有凸出的卡块，所述卡块能够与限位卡槽匹配连接。
此外，固化盒底部容纳槽的宽度与样品载玻片的尺寸匹配，在加胶臂压紧固化杯的同时，固化盒底部容纳槽将样品载玻片卡紧防止样品载玻片窜动。所述贯穿空的尺寸与固化杯的尺寸匹配，固化杯周向上设置的卡块与固化盒上的限位卡槽匹配连接，从而限制固化杯稳固安装在固化盒上，不会窜动也不会转动，从而不影响后续滴胶过程，使得滴胶位置保持固定。所述固化盒底部的导向弹簧柱在加胶臂压紧固化杯于样品载玻片表面时处于蓄能状态，当加胶臂抬升时，导向弹簧柱释放蓄能会自动将固化杯抬起，从而使固化杯与样品载玻片分离。所述固化杯底部镂空为圆形，使得封片胶在样本载玻片上固定位置形成圆形固化层。
优选地，在一些实施方式中，可以将紫外灯的外部设置有灯罩，所述灯罩位于样本滑轨上方，滴胶后的样本载玻片在灯罩内接受紫外灯固化。
所述加胶臂内设置有封片胶容纳装置、注射系统、抽吸系统以及废胶容纳装置，所述试剂针包括加胶针、吸胶针；所述封片胶容纳装置连接注射系统，注射系统连接加胶针，所述吸胶针连接抽吸系统，抽吸系统连接废胶容纳装置。所述吸胶针为一个或多个，所述多个吸胶针均匀设置在加胶针的周向上；所述注射系统上设置有计量装置。
所述注射系统能够将封片胶容纳装置内的封片胶通过加胶针滴加到样本载玻片表面，注射系统注射加胶的体积通过计量装置计量，并通过计量装置控制滴胶的体积；所述抽吸系统能够将多余的封片胶通过吸胶针抽吸到废胶容纳装置，在一个实施例中，加胶针的周向上均匀设置有四个吸胶针，即第一吸胶针、第二吸胶针、第三吸胶针以及第四吸胶针。
实施例3
在本实施例中，所述的恒温全自动化细胞制片一体化装置还包括多孔板过滤装置，所述多孔板过滤装置用于对玻片进行正压吹气。
所述多孔板过滤装置包括多孔板、夹爪、移动组件、导向块、吹气正压块以及机架；所述移动组件、导向块、吹气正压块均设置在机架上，夹爪连接移动组件并能够在移动组件的作用下运动，夹爪能够抓取多孔板，多孔板安装在导向块上，导向块位于吹气正 压块的下方。所述移动组件包括X轴滑轨、Y轴滑轨，Y轴滑轨安装在X滑轨上并能够在X轴滑轨上运动，夹爪安装在Y轴滑轨上并能够在Y轴滑轨上运动。还包括堆栈组件，所述堆栈组件包括多层放置位置，所述放置位置能够放置多孔板。
所述导向块活动连接机架，导向块能够在吹气正压块的下方平动和转动。所述吹气正压块活动连接机架，吹气正压块能够在导向块的上方平动和/或转动；所述吹气正压块能够通过管路连接外部气体。
所述多孔板包括孔格、滤膜以及滤液盘，滤膜连接在孔格的底部，滤液盘可拆卸连接在滤膜下方，多孔板两侧边沿外翻形成夹持位置。所述多孔板边沿底部设置有一个或多个卡条，所述夹爪上设置有一个或多个卡槽，夹爪夹持在多孔板夹持位置时，卡条与卡槽一一对应匹配连接；所述多孔板一侧边沿上设置切角形成放置标志。所述孔格为多个，多个孔格排列成多排多列；所述孔格为门拱形、T形或者三角形。所述滤膜过滤孔直径为1-50微米。
优选地，在一些实施方式中，多孔板包括24个门拱形孔格，排列成6排4列，滤膜采用筛网，筛网通过热焊工艺焊接在孔格底部，门拱形孔格圆弧部直径为φ4-50毫米，门拱形孔格直线边的宽度4-50毫米，门拱形孔格深度5-40毫米，多孔板的液体加注量为20-3000微升，多孔板材质为PES(聚醚砜树脂)、PE(聚乙烯)或PET(聚对苯二甲酸乙二醇酯)。
需要说明的是，本发明的保护范围中现有技术部分并不局限于本申请文件所给出的实施例，所有不与本发明的方案相矛盾的现有技术，包括但不局限于在先专利文献、在先公开出版物，在先公开使用等等，都可纳入本发明的保护范围。
此外，本案中各技术特征的组合方式并不限本案权利要求中所记载的组合方式或是具体实施例所记载的组合方式，本案记载的所有技术特征可以以任何方式进行自由组合或结合，除非相互之间产生矛盾。
还需要注意的是，以上所列举的实施例仅为本发明的具体实施例。显然本发明不局限于以上实施例，随之做出的类似变化或变形是本领域技术人员能从本发明公开的内容直接得出或者很容易便联想到的，均应属于本发明的保护范围。
以上对本发明的具体实施例进行了描述。需要理解的是，本发明并不局限于上述特定实施方式，本领域技术人员可以在权利要求的范围内做出各种变化或修改，这并不影响本发明的实质内容。在不冲突的情况下，本申请的实施例和实施例中的特征可以任意相互组合。

Claims (10)

  1. 一种恒温全自动化细胞制片一体化装置，其特征在于，其包括：
    旋转沉降盘，所述旋转沉降盘包括沉降盘盘座以及通过固定轴与沉降盘盘体连接的转盘盘体，其中，所述沉降盘盘座底部设有温控组件，沉降盘盘座侧壁依次排列设有玻片入口、玻片出口以及清洗加样入口；沿转盘盘体圆周边缘均匀设置有至少一个或多个沉降杯容置槽，以容置沉降杯；所述转盘盘体间歇性地绕所述固定轴转动，所述转盘盘体与旋转沉降盘盘座间形成至少四个玻片卡槽，待加样玻片或是已加样玻片适配地容置于所述玻片卡槽内；
    加片机构，所述加片机构设于靠近所述旋转沉降盘的玻片入口位置，所述加片机构将待加样玻片由所述玻片入口位置推送入旋转沉降盘内；
    清洗加样集成装置，所述清洁加液集成装置设于所述旋转沉降盘旁侧，所述清洗加液集成装置包括清洗液组件以及加样组件，所述清洗液组件以及加样组件随同转盘盘体的间歇转动同时分别向不同的一个或多个沉降杯内加液；
    出片机构，所述出片机构设于靠近所述旋转沉降盘的玻片出口位置，所述出片机构将已加样玻片由所述玻片出口位置移出。
  2. 根据权利要求1所述的恒温全自动化细胞制片一体化装置，其特征在于，所述沉降盘盘座外壁及底部设有保温层，所述保温层接近所述转盘盘体的内层设有保温棉，而远离转盘盘体的外层设有金属包层。
  3. 根据权利要求1所述的恒温全自动化细胞制片一体化装置，其特征在于，所述温控组件包括温控元件以及与温控元件连接的加热装置，所述加热装置设于所述沉降盘盘座对应沉降杯容置槽的位置，所述温控元件包括电阻调节器。
  4. 根据权利要求1所述的恒温全自动化细胞制片一体化装置，其特征在于，温控组件控制所述沉降杯容置槽内的沉降杯温度为20～60℃。
  5. 根据权利要求1所述的恒温全自动化细胞制片一体化装置，其特征在于，所述加片机构包括开设有开口的玻片匣以及推送装置，所述推送装置将存储于玻片匣内的玻片经所述开口推送至玻片入口位置，并由所述玻片入口进入旋转沉降盘的玻片卡槽内。
  6. 根据权利要求1所述的恒温全自动化细胞制片一体化装置，其特征在于，所述清洗加样集成装置还包括机械臂，所述机械臂一端与Z向调节机构连接，另一端清洗液组件与加样组件的流体连接；
    所述Z向调节机构沿竖直方向往复运动，以调节机械臂相对于转盘盘体的相对高度。
  7. 根据权利要求6所述的恒温全自动化细胞制片一体化装置，其特征在于，所述机械臂具有弧形部。
  8. 根据权利要求1所述的恒温全自动化细胞制片一体化装置，其特征在于，所述出片机构包括出片拨叉以及与出片拨叉连接的驱动机构。
  9. 根据权利要求所述的恒温全自动化细胞制片一体化装置，其特征在于，所述沉降杯容置槽与玻片卡槽间隔设置，当转盘盘体间歇转动时，玻片入口、玻片出口以及清洗加样入口位置处的玻片卡槽与沉降杯容置槽间的空间连通。
  10. 一种采用上述权利要求1-9中任意一项所述的恒温全自动化细胞制片一体化装置使用方法，其特征在于，包括如下步骤：
    步骤S1：待加样玻片由玻片入口进入旋转沉降盘内，并随同转盘盘体间歇转动；
    步骤S2：清洗加样集成装置中的清洗液组件以及加样组件随同转盘盘体的间歇转动同时分别向不同的一个或多个沉降杯内加液，此时，加样组件通过沉降杯向待加样玻片加样，而清洗组件则对已加完样的沉降杯进行清洗，已加完样的已加样玻片则由玻片出口移出。
    、
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