WO2022062386A1

WO2022062386A1 - Energy-saving self-stirring bioreactor

Info

Publication number: WO2022062386A1
Application number: PCT/CN2021/089818
Authority: WO
Inventors: 沈超锋
Original assignee: 湖州越彬智能科技有限公司
Priority date: 2020-09-24
Filing date: 2021-04-26
Publication date: 2022-03-31
Also published as: CN112080397A

Abstract

Disclosed is an energy-saving self-stirring bioreactor, comprising a reaction box. A ring-shaped heating plate is embedded on an inner wall of the reaction box. The bottom part within the reaction box is, by means of a rotating shaft, in sealed rotating connection with a stirring rod. The lower end of the reaction box is provided with a circular recess. The lower end of the rotating shaft penetrates through the lower end of the reaction box and is fixedly connected to a disc, the disc being provided with a cavity. A thermoelectric power generation sheet is rotatably connected, by means a cross bar, to the inside of the cavity. A sidewall of the heating plate is connected, by means of a heat conduction strip, to an inner top part of the cavity. A plurality of heat dissipation sheets are fixedly connected to the bottom part of the cavity. An electromagnet is embedded in the disk. In the present invention, by means of generating an electrical current on the thermoelectric power generation sheet, the electromagnet is electrified to generate magnetism. Since a permanent magnet and an electromagnet repel, the electromagnet rotates forward under the magnetic repulsion force of the permanent magnet, thereby driving the rotating shaft to rotate such that the microorganisms in the reaction box are stirred by the stirring rod and are heated evenly, thus accelerating the reaction efficiency thereof.

Description

An energy-saving self-stirring bioreactor

technical field

The invention relates to the technical field of biological engineering treatment, in particular to an energy-saving self-stirring bioreactor.

Background technique

Bioreactors are equipment used for microbial cultivation. Small bioreactors are often used in laboratories for microbial cultivation. In the process of microbial cultivation, in order to ensure the normal reaction of microorganisms, the microorganisms need to be heated at a constant temperature and Add nutrient solution.

In the actual operation of the laboratory, when the microorganisms need to be cultivated, the microorganisms are often stirred artificially from time to time, which makes the experimental operation of the microorganisms more cumbersome, and when adding nutrient solution to the reactor, it is always It is necessary to open the lid on the reactor to add, so that the microorganisms in the reactor will be in direct contact with the air, which makes the microorganisms easily infected, thereby affecting the experimental results.

To this end, we propose an energy-saving self-stirring bioreactor.

SUMMARY OF THE INVENTION

The purpose of the present invention is to propose an energy-saving self-stirring bioreactor in order to solve the shortcomings existing in the prior art.

In order to achieve the above object, the present invention adopts the following technical solutions:

An energy-saving self-stirring bioreactor includes a reaction box, an annular heating plate is embedded in the inner wall of the reaction box, a stirring rod is rotatably connected to the inner bottom of the reaction box through a rotary shaft seal, and a lower end of the reaction box is provided with a circular groove, the lower end of the rotating shaft penetrates the lower end of the reaction box and is fixedly connected with a disc, a cavity is opened on the disc, and a thermoelectric power generation sheet is rotatably connected in the cavity through a cross bar, and the side wall of the heating plate passes through The heat-conducting strip is connected to the top of the cavity, the bottom of the cavity is fixedly connected with a plurality of heat sinks, the disc is embedded with an electromagnet, the thermoelectric power generation piece is coupled and connected to the electromagnet, and the inner wall of the circular groove is connected to the electromagnet. A permanent magnet is symmetrically embedded, and the electromagnet and the permanent magnet repel each other. A driving mechanism for driving the disc to rotate forward and reverse is installed in the circular groove. The lower end of the reaction box is provided with a liquid storage cavity. The liquid storage cavity The inside is filled with nutrient solution, and a pump mechanism for pumping the nutrient solution into the reaction box is installed on the reaction box.

Preferably, the driving mechanism includes two racks that are symmetrically and fixedly connected to the inner wall of the circular slot, the two racks are located on the inner wall of the circular slot away from the permanent magnet, and one end of the transverse rod away from the thermoelectric sheet penetrates the disc The side wall is fixedly connected with gears.

Preferably, the pumping mechanism includes a magnetic sliding plug, an arc-shaped cavity is opened at the lower end of the reaction box, the inner wall of the arc-shaped cavity is fixedly connected to the side wall of the magnetic sliding plug through a spring, and the inner wall of the arc-shaped cavity is connected by a one-way The suction pipe is connected with the inner wall of the liquid storage cavity, and the inner wall of the arc-shaped cavity is connected with the inner bottom of the reaction box through the one-way liquid outlet hole.

The present invention has the following beneficial effects:

1. By setting the thermoelectric power generation sheet, the heat conduction strip, the heat sink, the electromagnet and the permanent magnet, when the heating plate heats the reaction box, under the action of the heat conduction strip and the heat sink, a temperature difference is generated on both sides of the thermoelectric power generation sheet, thereby making A current is generated on the thermoelectric power generation sheet, so that the electromagnet is energized to generate magnetism. Since the permanent magnet and the electromagnet repel, the electromagnet rotates in the forward direction under the action of the magnetic repulsion of the permanent magnet, which in turn drives the rotating shaft to rotate, so that the stirring rod is responsive to the inside of the reaction box. The microorganisms are stirred, so that the microorganisms are heated evenly and the reaction efficiency is accelerated;

2. By setting the drive mechanism, when the electromagnet rotates to the limit position opposite to the permanent magnet under the action of the magnetic repulsion of the permanent magnet, during the rotation of the disc, the gear meshes with the rack, and then the gear rotates to drive the thermoelectric sheet to rotate 180 degrees. °, so that the upper and lower ends of the thermoelectric sheet are reversed. Under the action of the heat conducting strip and the heat sink, the current generated on the thermoelectric sheet will be opposite to the initial state, so that the magnetism generated by the electrification of the electromagnet attracts the permanent magnet, and the electromagnetic The iron rotates in the opposite direction under the magnetic attraction of the permanent magnet, and drives the stirring rod to rotate again to stir the microorganism. When facing the permanent magnet plate, under the action of the heat conduction strip and the heat sink, the current direction on the thermoelectric power generation sheet is the same as the initial state, so that the electromagnet rotates forward again under the action of the magnetic repulsion of the permanent magnet, and so on. Make the disc drive the rotating shaft and the stirring rod to reciprocate forward and reverse in the reaction box, stir the microorganisms in the reaction box, avoid manual stirring, and reduce labor intensity;

3. By setting the pumping mechanism, when the electromagnet drives the disc to rotate in the forward direction, the electromagnet drives the magnetic sliding plug to slide on the inner wall of the arc-shaped cavity to the end away from the spring, and the nutrient solution in the arc-shaped cavity is discharged through one-way. The hole is squeezed into the reaction box. When the magnetism on the electromagnet disappears, the magnetic plug slides to the original position under the action of the spring, and the nutrient solution in the liquid storage cavity is sucked into the arc cavity through the one-way pipette. When the electromagnet drives the disc to reciprocate and rotate in the circular groove, the nutrient solution in the liquid storage chamber is continuously pumped into the reaction box, thereby maintaining the normal growth of microorganisms and avoiding manual opening of the lid on the reactor to add nutrients. liquid, so that the microorganisms are in direct contact with the air, so that the microorganisms are infected, which in turn affects the experimental results.

Description of drawings

Fig. 1 is the structural representation of a kind of energy-saving self-stirring bioreactor proposed by the present invention;

Fig. 2 is the structure enlarged schematic diagram at A place in Fig. 1;

Fig. 3 is the structure enlarged schematic diagram at B place in Fig. 1;

Fig. 4 is a top-view structural schematic diagram of a driving mechanism in an energy-saving self-stirring bioreactor proposed by the present invention;

FIG. 5 is a schematic top-view structural diagram of a pumping mechanism in an energy-saving self-stirring bioreactor proposed by the present invention.

In the picture: 1 reaction box, 2 feeding port, 3 discharging port, 4 observation port, 5 heating plate, 6 rotating shaft, 7 stirring rod, 8 circular groove, 9 disc, 10 cavity, 11 thermoelectric power generation sheet, 12 Thermal strip, 13 heat sink, 14 electromagnet, 15 permanent magnet, 16 gear, 17 rack, 18 liquid storage cavity, 19 arc cavity, 20 spring, 21 magnetic slide plug, 22 one-way suction pipe, 23 one-way Outlet hole.

detailed description

In order to make the above objects, features and advantages of the present invention more clearly understood, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific implementation disclosed below.

1-5, an energy-saving self-stirring bioreactor includes a reaction box 1, a feed port 2 is opened above the inner wall of the reaction box 1, a discharge port 3 is connected below the inner wall of the reaction box 1, and the upper end of the reaction box 1 is provided with There is an observation port 4, which is used to check the reaction situation of the microorganisms in the reaction box 1 at any time. The inner wall of the reaction box 1 is embedded with a ring-shaped heating plate 5. 1. The lower end is provided with a circular groove 8, and the lower end of the rotating shaft 6 penetrates the lower end of the reaction box 1 and is fixedly connected with a disc 9. The disc 9 is provided with a cavity 10, and a thermoelectric sheet 11 is connected in the cavity 10 through the rotation of the cross bar. The side wall of the plate 5 is connected with the inner top of the cavity 10 through the heat-conducting strip 12. Further, the heat-conducting strip 12 is made of soft material, and its length is long, so that the heat-conducting strip 12 can still be used for the cavity 10 when the disc 9 rotates. The inner bottom of the cavity 10 conducts heat conduction, and a plurality of heat sinks 13 are fixedly connected to the inner bottom of the cavity 10. It should be noted that due to the existence of the heat sink 13, the temperature of the bottom of the cavity 10 is lower, and the temperature of the top of the cavity 10 is higher than that of the cavity 10. The upper end and the lower end of the thermoelectric power generation sheet 11 will have a significant temperature difference, so that a current is generated on the thermoelectric power generation sheet 11. The disc 9 is embedded with an electromagnet 14, and the thermoelectric power generation sheet 11 is coupled with the electromagnet 14. The inner wall of the groove 8 is symmetrically embedded with a permanent magnet 15. The electromagnet 14 and the permanent magnet 15 repel each other. The circular groove 8 is equipped with a driving mechanism for driving the disc 9 to rotate in the positive and negative directions. The lower end of the reaction box 1 is provided with a liquid storage cavity 18. The liquid cavity 18 is filled with nutrient solution. Further, a one-way liquid inlet hole is opened on the inner wall of the liquid storage cavity 18 for adding nutrient solution to the liquid storage cavity 18. The reaction tank 1 is equipped with a pump for pumping the nutrient solution into the reaction tank. 1 inside the pump mechanism.

The drive mechanism includes two racks 17 symmetrically and fixedly connected to the inner wall of the circular groove 8, the two racks 17 are located on the inner wall of the circular groove 8 away from the permanent magnet 15, and one end of the crossbar away from the thermoelectric sheet 11 penetrates the side wall of the disc 9. And the gear 16 is fixedly connected. Further, in the initial state, the thermoelectric power generation sheet 11 generates current, so that the electromagnet 14 generates magnetic repulsion with the permanent magnet 15, and then the electromagnet 14 drives the disc under the action of the magnetic repulsion. 9 rotates forward, during the rotation of the disc 9, the gear 16 will contact the rack 17, and then the gear 16 will rotate 180° under the action of the rack 17, and then drive the thermoelectric sheet 11 to rotate 180°, making thermoelectric power generation The upper and lower ends of the sheet 11 are reversed, that is, the hot end of the thermoelectric sheet 11 is located at the bottom of the cavity 10 in the initial state, and the cold end of the thermoelectric sheet 11 is located at the top of the cavity 10. Under the action of , the temperature difference between the two ends of the thermoelectric power generation sheet 11 changes in a decreasing-balanced-increasing manner, and finally the current on the thermoelectric power generation sheet 11 is made opposite to the initial state, so that the electromagnet 14 is generated in the permanent magnet 15. The magnetism is attracted to each other, and then the electromagnet 14 drives the disc 9 to rotate in the opposite direction under the action of the magnetic attraction.

The pumping mechanism includes a magnetic slide plug 21, an arc-shaped cavity 19 is provided at the lower end of the reaction box 1, and the inner wall of the arc-shaped cavity 19 is fixedly connected to the side wall of the magnetic slide plug 21 through a spring 20. It should be noted that the arc-shaped cavity 19 is close to the spring 20. The inner wall of the arc cavity 19 is provided with a through hole that communicates with the outside world to balance the change of the pressure in the arc cavity 19 when the magnetic slide 21 slides on the inner wall of the arc cavity 19. The inner wall of the cavity 18 is connected, and the inner wall of the arc-shaped cavity 19 is connected to the inner bottom of the reaction box 1 through the one-way liquid outlet hole 23. It should be noted that the one-way pipette 22 only allows the nutrient solution to enter the arc-shaped cavity 19 from the liquid storage cavity 18. Inside, the one-way liquid outlet hole only allows the nutrient solution to enter the reaction box 1 from the arc-shaped cavity 19. Further, when the electromagnet 14 rotates to the limit position opposite to the permanent magnet 15 under the action of the magnetic repulsion of the permanent magnet 15, The thermoelectric sheet 11 rotates and the temperature difference on it changes in a decreasing-balance-increasing manner. When the temperature difference between the two ends of the thermoelectric sheet 11 is balanced, the current on the thermoelectric sheet 11 disappears, and then the magnetic force on the electromagnet 14 disappears. disappear. At this time, the magnetic slide plug 21 slides to the original position on the inner wall of the arc-shaped cavity 19 under the action of the spring 20. Then when the disc 9 rotates in the reverse direction, the position of the magnetic slide plug 21 remains unchanged. When rotating in the forward direction, the magnetic sliding plug 21 slides again to squeeze out the nutrient solution in the arc-shaped cavity 19 .

In the present invention, when the heating plate 5 heats the microorganisms in the reaction box 1 at a constant temperature, the heat-conducting strip 12 transfers the temperature on the heating plate 5 to the cavity 10, so that the temperature at the upper end of the thermoelectric sheet 11 increases. 10. A heat sink 13 is fixedly connected to the inner bottom, and then the lower end of the thermoelectric sheet 11 is lower in temperature under the action of the heat sink 13, so that a significant temperature difference is generated between the two ends of the thermoelectric sheet 11, and then a current is generated on the thermoelectric sheet 11. , so that the electromagnet 14 is energized to generate magnetic repulsion with the permanent magnet 15, and then the electromagnet 14 drives the disc 9 to rotate 180° in the forward direction under the action of the repulsion force of the permanent magnet 15, thereby making the rotating shaft fixedly connected to the upper end of the disc 9 6. Drive the stirring rod 7 to rotate in the forward direction to stir the microorganisms. During the forward rotation of the disc 9, the rack 17 fixedly connected to the inner wall of the circular groove 8 drives the gear 16 to rotate 180°, and then drives the coaxial fixed connection with the gear 16. The thermoelectric sheet 11 is rotated 180°, so that the upper and lower ends of the thermoelectric sheet 11 are exchanged, and then under the action of the heat sink 13 and the heat conducting strip 12, the temperature difference between the two ends of the thermoelectric sheet 11 is reduced-balanced-increased. Change, and finally make the current on the thermoelectric power generation piece 11 opposite to the initial state, so that the magnetism generated on the electromagnet 14 is attracted by the permanent magnet 15, and then the electromagnet 14 drives the disc 9 to rotate in the reverse direction under the action of the magnetic attraction force. , drive the stirring rod 7 to reversely rotate to stir the microorganisms. During the reverse rotation of the disc 9, the gear 16 drives the thermoelectric fins to rotate 180° again under the action of the rack 17. Under the action, the temperature difference between the two ends of the thermoelectric power generation sheet 11 is again reduced-balanced-increased, and finally the direction of the current on the thermoelectric power generation sheet 11 is the same as the initial state, thereby making the electromagnet 14 act on the magnetic repulsion of the permanent magnet 15. Rotate forward again, so that the disc 9 drives the stirring rod 7 to reciprocate forward and reverse rotation in the reaction box 1, and the microorganisms in the reaction box 1 are stirred;

When the disc 9 rotates in the forward direction, the electromagnet 14 drives the magnetic sliding plug 21 to slide on the inner wall of the arc-shaped cavity 19 to the end away from the spring 20, and squeezes the nutrient solution in the arc-shaped cavity 19 into the one-way liquid outlet hole 23. In the reaction box 1, the nutrient solution is added to the microorganisms in the reaction box 1. When the electromagnet 14 is rotated to the limit position, the temperature on the thermoelectric power generation sheet 11 is rotated by 180°, so that the temperature on it decreases-balance-increases. , when the temperature difference between the two ends of the thermoelectric sheet 11 is balanced, the current on the thermoelectric sheet 11 disappears, and then the magnetic force on the electromagnet 14 disappears. At this time, the magnetic slide 21 slides on the inner wall of the arc cavity 19 under the action of the spring 20 To the original position, the nutrient solution in the liquid storage cavity 18 is sucked into the arc-shaped cavity 19 through the one-way pipette 22, and then when the disc 9 rotates in the reverse direction, the position of the magnetic slide plug 21 remains unchanged. 9. When it rotates forward again, the magnetic slide plug 21 slides again to squeeze out the nutrient solution in the arc-shaped cavity 19. In this way, when the electromagnet 14 reciprocates forward and reverse rotation, the magnetic slide plug is in the magnetic attraction force of the electromagnet 14 and the spring. Under the action of the elastic force of 20, the inner wall of the arc-shaped cavity 19 slides back and forth, and the nutrient solution in the liquid storage cavity 18 is continuously pumped into the reaction box 1, thereby maintaining the normal growth of microorganisms.

The above description is only a preferred embodiment of the present invention, but the protection scope of the present invention is not limited to this. The equivalent replacement or change of the inventive concept thereof shall be included within the protection scope of the present invention.

Claims

An energy-saving self-stirring bioreactor, comprising a reaction box (1), characterized in that an annular heating plate (5) is embedded in the inner wall of the reaction box (1), and an inner bottom of the reaction box (1) is embedded A stirring rod (7) is sealed and rotatably connected through the rotating shaft (6), a circular groove (8) is formed at the lower end of the reaction box (1), and the lower end of the rotating shaft (6) penetrates the lower end of the reaction box (1) and is fixedly connected with a circular groove (8). A disc (9), a cavity (10) is opened on the disc (9), and a thermoelectric power generation sheet (11) is connected in the cavity (10) by rotating a cross bar, and the heating plate (5) side The wall is connected to the inner top of the cavity (10) through a heat conducting strip (12), a plurality of heat sinks (13) are fixedly connected to the inner bottom of the cavity (10), and an electromagnet is embedded in the disc (9). (14), the thermoelectric power generation sheet (11) is coupled and connected to the electromagnet (14), the inner wall of the circular groove (8) is symmetrically embedded with a permanent magnet (15), and the electromagnet (14) is connected to the permanent magnet (14). 15) Repel each other, a drive mechanism for driving the disc (9) to rotate forward and reverse is installed in the circular groove (8), and a liquid storage cavity (18) is opened at the lower end of the reaction box (1), and the liquid storage cavity (18) is filled with a nutrient solution, and a pump mechanism for pumping the nutrient solution into the reaction box (1) is installed on the reaction box (1).
An energy-saving self-stirring bioreactor according to claim 1, characterized in that the drive mechanism comprises two racks (17) symmetrically and fixedly connected to the inner wall of the circular groove (8), and the two racks (17) The bar (17) is located on the inner wall of the circular groove (8) away from the permanent magnet (15), and one end of the cross bar away from the thermoelectric sheet (11) penetrates the side wall of the disc (9) and is fixedly connected with a gear (16).
The energy-saving self-stirring bioreactor according to claim 1, characterized in that the pumping mechanism comprises a magnetic sliding plug (21), and an arc-shaped cavity (19) is opened at the lower end of the reaction box (1). The inner wall of the arc-shaped cavity (19) is fixedly connected to the side wall of the magnetic slide plug (21) through a spring (20), and the inner wall of the arc-shaped cavity (19) is connected to the liquid storage cavity (22) through a one-way pipette (22). 18) The inner wall is connected, and the inner wall of the arc-shaped cavity (19) is connected with the inner bottom of the reaction box (1) through the one-way liquid outlet hole (23).