WO2022062376A1 - 一种带有微电流刺激功能的细胞培养装置 - Google Patents

一种带有微电流刺激功能的细胞培养装置 Download PDF

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WO2022062376A1
WO2022062376A1 PCT/CN2021/088252 CN2021088252W WO2022062376A1 WO 2022062376 A1 WO2022062376 A1 WO 2022062376A1 CN 2021088252 W CN2021088252 W CN 2021088252W WO 2022062376 A1 WO2022062376 A1 WO 2022062376A1
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guide rail
slider
block
stage
magnetic
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French (fr)
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李刚
张淑军
赵婧媛
姚若彤
段立蓉
王恬
闫一欣
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Nantong Textile And Silk Industry Technology Research Institute
Suzhou University
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Nantong Textile And Silk Industry Technology Research Institute
Suzhou University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation

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  • the invention relates to the field of cell culture devices, in particular to a cell culture device with a microcurrent stimulation function.
  • Peripheral nerve damage is a common clinical disease, and its repair and regeneration has always been a difficult problem for neurosurgeons and materials experts.
  • the damaged nerve cannot be repaired by direct suture; autograft and allograft have disadvantages of secondary injury, limited source, cross infection and allogeneic rejection, therefore, researchers started artificial nerve transplantation research on things.
  • artificial nerve grafts are usually co-cultured with nerve cells and then transplanted to the nerve defect site. The latest research shows that when nerve cells are stimulated by microcurrent during the culture process, it is more conducive to proliferation, differentiation and growth, which can better promote the repair and regeneration of damaged axonal nerves.
  • the use of artificial electrical stimulation device to culture nerve cells in vitro can be more conducive to the tissue engineering of nerve conduits.
  • the conventional method for electrical stimulation of cells is to use primary batteries to generate electromotive force through the chemical reaction between the electrode and the electrolyte to generate a weak current.
  • Chinese Patent Publication No. CN201920576816.7
  • Chinese Patent provides a neural
  • the stimulation electrode assembly and the nerve electrical stimulation device generate a weak current by utilizing the chemical reaction between the electrode and the electrolyte in the primary battery.
  • This method of generating electric current through chemical reaction has defects. Since the chemical reaction rate will change with the reaction process, the generated electric current may change, and the electrolyte and electrode need to be replaced in a period of time.
  • the present invention proposes a cell culture device with a micro-current stimulation function, which can better promote various cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and Digestive tract cells, etc., are especially conducive to the proliferation, differentiation and growth of nerve cells, which can promote nerve repair and regeneration, and achieve more ideal results.
  • the present invention proposes a cell culture device with a microcurrent stimulation function, which includes a box body, an upper guide rail, an upper magnetic block, an upper slider, a lower guide rail, a lower magnetic block, a lower slider and a load. tower;
  • the box is a medical grade polymer box or a stainless steel box; the box includes an incubator and a control box; the incubator and the control box are arranged side by side;
  • the upper guide rail, upper magnet block, upper slider, lower guide rail, lower magnet block, lower slider and stage are all set in the incubator; the stage is located in the middle of the upper guide rail and the lower guide rail; the stage is horizontal set up;
  • the upper slider is slidably arranged on the upper guide rail, and the upper magnetic block is arranged on the lower surface of the upper slider;
  • the lower slider is slidably arranged on the lower guide rail, and the lower magnetic block is arranged on the upper surface of the lower slider;
  • the upper magnetic block and the lower magnetic block face each other.
  • One side of the stage has opposite magnetic poles;
  • control box Inside the control box is a control device for controlling the sliding of the upper slider and the lower magnetic block.
  • the distance between the stage and the upper guide rail and the lower guide rail is 1-50cm; the distance between the bottom surface of the upper magnet block and the stage is X; the distance between the upper surface of the lower magnet block and the stage is X; X is 0.5-50cm.
  • the sliding speed of the upper slider and the lower slider is 1-]00cm/s.
  • the two ends of the upper guide rail and the lower guide rail are respectively M end and N end;
  • the upper slider is slidably arranged at the M-end position of the upper guide rail, and the lower slider is slidably arranged at the M-end position of the lower guide rail;
  • the upper slider and the lower slider move synchronously, reciprocating from M to N, and then from N to M; the moving direction and speed of the upper slider and the lower slider are always the same;
  • the conductive device of length L and the culture dish for seeding cells are placed at the center of the stage;
  • the moving speed of the upper slider and the lower slider is V; the conductive device cuts the magnetic field line, and the calculation formula of the current I1 is:
  • ⁇ is the angle between the conducting device and the vertical magnetic field line
  • R is the resistance of the conductive device
  • B is the magnetic induction intensity between the upper magnet block and the lower magnet block
  • the conducting means are metal wires or metal tubes or metal rods.
  • the two ends of the upper guide rail and the lower guide rail are respectively M end and N end;
  • the upper slider is slidably arranged at the M end position of the upper guide rail, and the lower slider is slidably arranged at the N end position of the lower guide rail;
  • the upper slider reciprocates from M to N, and then from N to M;
  • the lower slider reciprocates from N to M, and then from M to N;
  • the moving direction of the upper slider and the lower slider are always opposite, and the moving speed is always the same;
  • the conductive device of length L and the culture dish for seeding cells are placed at the center of the stage;
  • the moving speed of the upper slider and the lower slider is V; the conductive device cuts the magnetic field line, and the calculation formula of the current I2 is:
  • I2 B ⁇ L ⁇ V ⁇ Sin ⁇ /R
  • ⁇ is the angle between the conducting device and the vertical magnetic field line
  • R is the resistance of the conductive device
  • B is the magnetic induction intensity between the upper magnet block and the lower magnet block
  • the conducting means are metal wires or metal tubes or metal rods.
  • the two ends of the upper guide rail and the lower guide rail are respectively M end and N end;
  • the lower slider is fixed at the M end position or the N end position of the lower guide rail;
  • the conductive device of length L and the culture dish for seeding cells are placed at the center of the stage; the moving speed of the upper slider is V; the conductive device cuts the magnetic field line, and the calculation formula of the current I3 is:
  • ⁇ is the angle between the conducting device and the vertical magnetic field line
  • R is the resistance of the conductive device
  • B is the magnetic induction intensity between the upper magnet block and the lower magnet block
  • the conducting means are metal wires or metal tubes or metal rods.
  • the present invention can better promote the proliferation, differentiation and growth of various cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, etc., especially nerve cells, through electrical stimulation, thereby promoting nerve repair and regeneration. , to achieve a more ideal effect;
  • the invention has the advantages of simple device structure, low production and maintenance costs, and easy industrialization promotion
  • the moving direction, trajectory and speed of the upper magnetic block and the lower magnetic block are set by the program to change the magnetic field, and the automatic control of the cell culture process is realized;
  • the present invention can maintain the stable change of the magnetic field, can generate a stable current, does not need to use a primary battery, does not need to use an electrolyte and an electrode, is continuously stable, and is green and environmentally friendly.
  • FIG. 1 is a schematic structural diagram of a cell culture device with a microcurrent stimulation function proposed by the present invention.
  • FIG. 2 is a schematic diagram of the first embodiment of the cell culture device with the microcurrent stimulation function proposed by the present invention.
  • FIG. 3 is a schematic diagram of the second embodiment of the cell culture device with the microcurrent stimulation function proposed by the present invention.
  • FIG. 4 is a schematic diagram of the third embodiment of the cell culture device with the microcurrent stimulation function proposed by the present invention.
  • Figure 5 is a cell culture device with a microcurrent stimulation function proposed by the present invention. After culturing in the device for three days, the growth state of nerve cells is good, indicating that the device proposed by the present invention is beneficial to the growth and proliferation of nerve cells.
  • Figure 6 shows the growth state of nerve cells after culturing for three days in a normal environment. It shows that the growth state of cells cultured in an ordinary environment is worse than that of cells cultured in the device of the present invention at the same time, and the device of the present invention is beneficial to the cultivation of nerve cells.
  • Figure 7 is a graph of the instantaneous electromotive force generated by the action of the device over time in the cell culture device with the microcurrent stimulation function proposed by the present invention.
  • the instantaneous electromotive force generated when the magnesium wire cuts the magnetic field line is about 0.35V, which lasts for about 4 seconds.
  • the electromotive force becomes 0.1V. It can be seen from the figure that the change is relatively regular, and is suitable for the theoretical calculation formula proposed by the present invention. It is proved that the device proposed by the present invention can generate microcurrent which is beneficial to the culture of nerve cells.
  • a cell culture device with a microcurrent stimulation function proposed by the present invention includes a box body 4, an upper guide rail 11, an upper magnetic block 21, an upper slider 31, a lower guide rail 12, and a lower magnetic block. block 22, lower slider 32 and stage 6;
  • the box body 4 is a medical grade polymer box body or a stainless steel box body; the box body 4 includes an incubator 41 and a control box 42; the incubator 41 and the control box 42 are arranged side by side;
  • the upper guide rail 11, the upper magnetic block 21, the upper slider 31, the lower guide rail 12, the lower magnetic block 22, the lower slider 32 and the stage 6 are all arranged in the incubator 41;
  • the middle position of the guide rail 12; the stage 6 is set horizontally;
  • the upper slider 31 is slidably arranged on the upper guide rail 11, and the upper magnetic block 21 is arranged on the lower surface of the upper slider 31; the lower slider 32 is slidably arranged on the lower guide rail 12, and the lower magnetic block 22 is arranged on the upper surface of the lower slider 32;
  • the magnetic poles of the upper magnetic block 21 and the lower magnetic block 22 facing the stage 6 are opposite;
  • a control device for controlling the sliding of the upper slider 31 and the lower magnet block 22 is provided inside the control box 42 .
  • the distance between the stage 6 and the upper guide rail 11 and the lower guide rail 12 is 1-50 cm;
  • the distance between the bottom surface of the upper magnetic block 21 and the stage 6 is X; the distance between the upper surface of the lower magnetic block 22 and the stage 6 is X; X is 0.5-50 cm.
  • the sliding speed of the upper slider 31 and the lower slider 32 is 1-100 cm/s.
  • magnetism-generated electricity when a part of the conductor of the closed circuit performs the motion of cutting the magnetic field line, an induced current will be generated on the conductor.
  • conductive materials such as medical human body pipes, metal wires, etc.
  • the purpose of automatically cutting the magnetic field lines can be achieved, thereby generating induced currents that allow cells to accept Electrical stimulation to better promote their proliferation, differentiation and growth.
  • the device based on the principle of "magnetically generated electricity” does not have a chemical reaction, so there is no change in the current generated due to the change of the chemical reaction rate, and it avoids the need to replace the electrolyte and electrodes. troubled.
  • a stable current can be generated by uniformly cutting the magnetic field through the reciprocating motion of the cell culture dish or the magnetic device, which is especially suitable for the device for promoting the regeneration of damaged nerves based on the principle of "magnetism-generated electricity";
  • the culture dish and the conductive device are placed in the middle of the upper magnetic block 21 and the lower magnetic block 22, and the magnetic field is changed by the movement of the upper magnetic block 21 and the lower magnetic block 22, and the conductive device automatically cuts the magnetic field lines; then, by changing the magnetic field,
  • the induction current is generated around the conductive device, so that the nerve cells receive electrical stimulation, and the nerve cells are better cultivated, so as to achieve the purpose of promoting the repair and regeneration of damaged nerves.
  • the present invention can promote the proliferation, differentiation and growth of nerve cells by conducting electrical stimulation in various cell culture processes, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, etc., so as to promote nerve repair. and regeneration benefits.
  • the two ends of the upper guide rail 11 and the lower guide rail 12 are set as the M end and the N end respectively;
  • the upper slider 31 is slidably arranged at the M end position of the upper guide rail 11, and the lower slider 32 is slidably arranged at the M end position of the lower guide rail 12;
  • the upper slider 31 and the lower slider 32 move synchronously, reciprocating from M to N, and then from N to M; the moving direction and speed of the upper slider 31 and the lower slider 32 are always the same;
  • a conductive device with a length of L and a culture dish for seeding cells are placed at the center of the stage 6;
  • the moving speed of the upper slider 31 and the lower slider 32 is V; the conductive device cuts the magnetic field line, and the calculation formula of the current I1 is:
  • ⁇ is the angle between the conducting device and the vertical magnetic field line
  • R is the resistance of the conductive device
  • B is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;
  • the conducting means are metal wires or metal tubes or metal rods.
  • the role of the current is to stimulate the growth of nerve cells around the conduit, thereby accelerating the regeneration and repair of damaged tissue.
  • the two ends of the upper guide rail 11 and the lower guide rail 12 are set as the M end and the N end respectively;
  • the upper slider 31 is slidably arranged at the M end position of the upper guide rail 11, and the lower slider 32 is slidably arranged at the N end position of the lower guide rail 12;
  • the upper slider 31 reciprocates from M to N, and then from N to M;
  • the lower slider 32 reciprocates from N to M, and then from M to N;
  • the moving direction of the upper slider 31 and the lower slider 32 are always opposite, and the moving speed is always the same;
  • a conductive device with a length of L and a culture dish for seeding cells are placed at the center of the stage 6;
  • the moving speed of the upper slider 31 and the lower slider 32 is V; the conductive device cuts the magnetic field line, and the calculation formula of the current I2 is:
  • I2 B ⁇ L ⁇ V ⁇ Sin ⁇ /R
  • ⁇ is the angle between the conducting device and the vertical magnetic field line
  • R is the resistance of the conductive device
  • B is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;
  • the conducting means are metal wires or metal tubes or metal rods.
  • the effect of current can stimulate damaged axonal nerve cells around the catheter, promote the proliferation, differentiation and growth of damaged axonal nerve cells, and accelerate the regeneration and repair of damaged axonal nerves.
  • the two ends of the upper guide rail 11 and the lower guide rail 12 are set as the M end and the N end respectively;
  • the lower slider 32 is fixedly arranged at the M end position or the N end position of the lower guide rail 12;
  • the upper slider 31 reciprocates from M to N, and then from N to M; the lower slider 32 never moves;
  • the conductive device of length L and the culture dish for seeding cells are placed at the center of the stage 6; the moving speed of the upper slider 31 is V; the conductive device cuts the magnetic field line, and the calculation formula of the current I3 is:
  • ⁇ is the angle between the conducting device and the vertical magnetic field line
  • R is the resistance of the conductive device
  • B is the magnetic induction intensity between the upper magnetic block 21 and the lower magnetic block 22;
  • the conducting means are metal wires or metal tubes or metal rods.
  • the effect of current can stimulate the growth of nerve cells around the conduit to a certain extent, so that the long-distance and long-term damaged peripheral nerves can be regenerated and repaired faster, and nerve cells can be cultured in vitro to promote nerve cell proliferation. , differentiation and growth.
  • the present invention has the following advantages:
  • the present invention can better promote the proliferation, differentiation and growth of various cells, such as tendons, ligaments, nerves, blood vessels, skin, muscles and digestive tract cells, etc., especially nerve cells, through electrical stimulation, thereby promoting nerve repair and regeneration. , to achieve a more ideal effect;
  • the invention has the advantages of simple device structure, low production and maintenance costs, and easy industrialization promotion
  • the motion trajectories of the upper magnetic block 21 and the lower magnetic block 22 are set by the program to change the magnetic field and realize the automatic control of the cell culture process;
  • the invention according to the principle of "magnetism to generate electricity", can maintain the stable change of the magnetic field, can generate a stable current, does not need to use primary batteries, does not need to use electrolytes and electrodes, and is green and environmentally friendly.

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Abstract

本申请提供一种带有微电流刺激功能的细胞培养装置，其包括箱体、上导轨、上磁块、上滑块、下导轨、下磁块、下滑块和载物台；箱体为医用级聚合物箱体或不锈钢箱体；箱体包括培养箱和控制箱；培养箱和控制箱并排设置；上导轨、上磁块、上滑块、下导轨、下磁块、下滑块和载物台均设置在培养箱内；载物台位于上导轨与下导轨的中间位置处；载物台水平设置；上滑块滑动设置在上导轨上，上磁块设置在上滑块的下表面；下滑块滑动设置在下导轨上，下磁块设置在下滑块的上表面；上磁块与下磁块磁极相反。通过在多种细胞培养过程中进行电刺激，如肌腱、韧带、神经、血管、皮肤、肌肉和消化道细胞等，尤其是能够促进神经细胞的增殖、分化和生长，达到促进神经修复和再生的有益效果。

Description

一种带有微电流刺激功能的细胞培养装置 技术领域
本发明涉及细胞培养装置领域，尤其涉及一种带有微电流刺激功能的细胞培养装置。
背景技术
周围神经受损是临床上常见的病症，它的修复和再生也一直都是困扰神经外科专家和材料专家的难点问题。当长距离和长时间受损时，受损神经无法进行直接缝合修复；自体移植和异体移植存在二次损伤、来源有限、交叉感染和异体排异的弊端，因此，研究人员开始了人工神经移植物的研究。在实际应用中，人工神经移植物通常会与神经细胞共培养后移植到神经缺损部位。最新的研究表明，当神经细胞在培养过程中受到微电流刺激时，更加有利于增殖、分化和生长，从而可以更好地促进受损轴突神经的修复和再生。
采用人工电刺激装置对神经细胞进行体外培养能够更有利于神经导管的组织工程化。目前，用于细胞电刺激的常规方法是使用原电池，通过电极和电解液之间的化学反应产生电动势进而产生微弱电流，例如，中国专利(公开号：CN201920576816.7)提供了一种神经电刺激电极组件及神经电刺激装置，通过利用原电池中电极和电解液间的化学反应产生微弱电流。此种通过化学反应产生电流的方式存在缺陷，由于化学反应速率会随着反应过程发生改变，可能使产生的电流发生变化，且需要在一段周期内更换电解液和电极。此外，原电池中可能会产生副反应，导致电池的充放电效率降低、容量寿命损失和性能下降，例如，中国专利(公开号：CN92242285.0)提供了一种由连体双缸、盐桥、铜片、锌片、特制小灯泡和正负极接线柱等组成的铜锌原电池演示仪，此种方法以锌作原电池的负极。当采用纯锌作电极时，锌片上有少量气泡产生；当采用不纯的锌作电极时，锌片上有更多气泡产生。这是由于在锌片上的锌与表面杂质形成许多微小的原电池，发生了副反应。不能够为电刺激装置提供稳定持续的电源，不利于细胞的正常培养。
因此，如何解决上述缺陷，需要解决如何提供一种具有持续稳定和安全环保的电刺激功能的细胞培养装置。
发明内容
(一)发明目的
为解决背景技术中存在的技术问题，本发明提出一种带有微电流刺激功能的细胞培养装置，通过电刺激更好地促进多种细胞，如肌腱、韧带、神经、血管、皮肤、肌肉和消化道细胞等，尤其有利于神经细胞的增殖、分化和生长，从而可以促进神经修复和再生，达到更理想的效果。
(二)技术方案
为解决上述问题，本发明提出了一种带有微电流刺激功能的细胞培养装置，包括箱体、上导轨、上磁块、上滑块、下导轨、下磁块、下滑块和载物台；
箱体为医用级聚合物箱体或不锈钢箱体；箱体包括培养箱和控制箱；培养箱和控 制箱并排设置；
上导轨、上磁块、上滑块、下导轨、下磁块、下滑块和载物台均设置在培养箱内；载物台位于上导轨与下导轨的中间位置处；载物台水平设置；
上滑块滑动设置在上导轨上，上磁块设置在上滑块的下表面；下滑块滑动设置在下导轨上，下磁块设置在下滑块的上表面；上磁块与下磁块朝向载物台的一面磁极相反；
控制箱内部设有用于控制上滑块及下磁块滑动的控制装置。
优选的，载物台距离上导轨以及下导轨之间的距离为1-50cm；上磁块底面与载物台之间的距离为X；下磁块上表面与载物台之间的距离为X；X为0.5-50cm。
优选的，上滑块以及下滑块的滑动速度为1-]00cm/s。
优选的，设上导轨以及下导轨的两端分别为M端和N端；
初始状态下：上滑块滑动设置在上导轨的M端位置处，下滑块滑动设置在下导轨的M端位置处；
运动状态下，上滑块与下滑块同步移动，沿由M向N，再由N向M的往复运动；上滑块与下滑块移动方向和移动速度始终相同；
长度为L的导电装置与铺种细胞的培养皿放置在载物台的中心位置处；
上滑块及下滑块的移动速度为V；导电装置切割磁感线，电流I1的计算公式为：
I1＝B×L×V×Sinα/R；
α为导电装置与垂直磁感线之间的夹角；
R为导电装置的电阻；
B为上磁块与下磁块之间的磁感应强度；
导电装置为金属丝或金属管或金属杆。
优选的，设上导轨以及下导轨的两端分别为M端和N端；
初始状态下：上滑块滑动设置在上导轨的M端位置处，下滑块滑动设置在下导轨的N端位置处；
运动状态下，上滑块沿由M向N，再由N向M的往复运动；下滑块沿由N向M，再由M向N的往复运动；
上滑块与下滑块移动方向始终相反，移动速度始终相同；
长度为L的导电装置与铺种细胞的培养皿放置在载物台的中心位置处；
上滑块及下滑块的移动速度为V；导电装置切割磁感线，电流I2的计算公式为：
I2＝B×L×V×Sinα/R；
α为导电装置与垂直磁感线之间的夹角；
R为导电装置的电阻；
B为上磁块与下磁块之间的磁感应强度；
导电装置为金属丝或金属管或金属杆。
优选的，设上导轨以及下导轨的两端分别为M端和N端；
初始状态下：下滑块固定设置在下导轨的M端位置处或N端位置处；
运动状态下，上滑块沿由M向N，再由N向M的往复运动；下滑块始终不动；
长度为L的导电装置与铺种细胞的培养皿放置在载物台的中心位置处；上滑块的移动速度为V；导电装置切割磁感线，电流I3的计算公式为：
I3＝B×L×V×Sinα/R；
α为导电装置与垂直磁感线之间的夹角；
R为导电装置的电阻；
B为上磁块与下磁块之间的磁感应强度；
导电装置为金属丝或金属管或金属杆。
本发明，通过电刺激更好地促进多种细胞，如肌腱、韧带、神经、血管、皮肤、肌肉和消化道细胞等，尤其是神经细胞的增殖、分化和生长，从而可以促进神经修复和再生，达到更理想的效果；
本发明，装置结构简单，生产和维修保养成本低，易于工业化推广；
本发明，通过程序设定上磁块和下磁块的运动方向、轨迹、速度以改变磁场，并实现细胞培养过程的自动化控制；
本发明，根据“磁生电”原理，可以维持磁场的稳定变化，可产生稳定的电流，无需使用原电池，无需使用电解液和电极，持续稳定，绿色环保。
附图说明
图1为本发明提出的带有微电流刺激功能的细胞培养装置的结构示意图。
图2为本发明提出的带有微电流刺激功能的细胞培养装置的第一个实施例示意图。
图3为本发明提出的带有微电流刺激功能的细胞培养装置的第二个实施例示意图。
图4为本发明提出的带有微电流刺激功能的细胞培养装置的第三个实施例示意图。
图5为本发明提出的带有微电流刺激功能的细胞培养装置，经过该装置中培养三天后的神经细胞生长状态良好，说明本发明提出的装置有利于神经细胞的生长和增殖。
图6为对照普通环境下，经过培养三天后的神经细胞的生长状态。说明经过普通环境培养的细胞相比于在本发明所述装置中相同时间培养的细胞的生长状态较差，说明本发明提出的装置有利于神经细胞的培养。
图7为本发明提出的带有微电流刺激功能的细胞培养装置中，数量为10根、长度规格为10cm的镁丝在经过装置作用过程中所产生的瞬间电动势随着时间变化图。当上、下导轨的磁铁同向匀速直线运动时，镁丝切割磁感线时产生的瞬间电动势约为0.35V，大约持续4秒钟，在变换方向的瞬间，电动势变为0.1V。从图中可以看出该变化较为规律，适用于本发明提出的理论计算公式。证明了本发明提出的装置能够产生有利于神经细胞培养的微电流。
具体实施方式
为使本发明的目的、技术方案和优点更加清楚明了，下面结合具体实施方式并参照附图，对本发明进一步详细说明。应该理解，这些描述只是示例性的，而并非要限制本发明的范围。此外，在以下说明中，省略了对公知结构和技术的描述，以避免不必要地混淆本发明的概念。
如图1-7所示，本发明提出的一种带有微电流刺激功能的细胞培养装置，包括箱体4、上导轨11、上磁块21、上滑块31、下导轨12、下磁块22、下滑块32和载物台6；
箱体4为医用级聚合物箱体或不锈钢箱体；箱体4包括培养箱41和控制箱42；培养箱41和控制箱42并排设置；
上导轨11、上磁块21、上滑块31、下导轨12、下磁块22、下滑块32和载物台6均设置在培养箱41内；载物台6位于上导轨11与下导轨12的中间位置处；载物台6水平设置；
上滑块31滑动设置在上导轨11上，上磁块21设置在上滑块31的下表面；下滑块32滑动设置在下导轨12上，下磁块22设置在下滑块32的上表面；上磁块21与下磁块22朝向载物台6的一面磁极相反；
控制箱42内部设有用于控制上滑块31及下磁块22滑动的控制装置。
在一个可选的实施例中，载物台6距离上导轨11以及下导轨12之间的距离为1-50cm；
上磁块21底面与载物台6之间的距离为X；下磁块22上表面与载物台6之间的距离为X；X为0.5-50cm。
在一个可选的实施例中，上滑块31以及下滑块32的滑动速度为1-100cm/s。
本发明中，根据“磁生电”原理，当闭合电路的一部分导体做切割磁感线运动时，在导体上便会产生感应电流。在磁场环境下，只要在细胞周围放置具有导电性的材料(如医用人体管道、金属丝等)，并改变磁场时，就可以达到自动切割磁感线的目的，进而产生感应电流，使细胞接受电刺激，从而更好地促进其增殖、分化和生长。相对于原电池的方法，“磁生电”原理的装置由于不存在化学反应，也就不存在因为化学反应速率的变化而使产生的电流发生变化，而且，避免了需要更换电解液和电极的困扰。
本发明中，通过细胞培养皿或磁力装置的往复运动，均匀切割磁场，即可产生稳定的电流，尤其适用于基于“磁生电”原理的促受损神经再生装置；将铺种有神经细胞的培养皿和导电装置放置在上磁块21与下磁块22中间，通过上磁块21与下磁块22的移动改变磁场，并使导电装置自动切割磁感线；进而，通过改变磁场，使导电装置周围产生感应电流，使神经细胞接受电刺激，更好地对神经细胞进行培养，从而达到促进受损神经修复和再生的目的。
本发明，通过在多种细胞培养过程中进行电刺激，如肌腱、韧带、神经、血管、皮肤、肌肉和消化道细胞等，尤其是能够促进神经细胞的增殖、分化和生长，达到促进神经修复和再生的有益效果。
实施例1
本发明中，设上导轨11以及下导轨12的两端分别为M端和N端；
初始状态下：上滑块31滑动设置在上导轨11的M端位置处，下滑块32滑动设置在下导轨12的M端位置处；
运动状态下，上滑块31与下滑块32同步移动，沿由M向N，再由N向M的往复运动；上滑块31与下滑块32移动方向和移动速度始终相同；
长度为L的导电装置与铺种细胞的培养皿放置在载物台6的中心位置处；
上滑块31及下滑块32的移动速度为V；导电装置切割磁感线，电流I1的计算公式为：
I1＝B×L×V×Sinα/R；
α为导电装置与垂直磁感线之间的夹角；
R为导电装置的电阻；
B为上磁块21与下磁块22之间的磁感应强度；
导电装置为金属丝或金属管或金属杆。
需要说明的是，电流的作用是刺激导管周围神经细胞的生长，从而加快受损组织的再生与修复。
实施例2
本发明中，设上导轨11以及下导轨12的两端分别为M端和N端；
初始状态下：上滑块31滑动设置在上导轨11的M端位置处，下滑块32滑动设置在下导轨12的N端位置处；
运动状态下，上滑块31沿由M向N，再由N向M的往复运动；下滑块32沿由N向M，再由M向N的往复运动；
上滑块31与下滑块32移动方向始终相反，移动速度始终相同；
长度为L的导电装置与铺种细胞的培养皿放置在载物台6的中心位置处；
上滑块31及下滑块32的移动速度为V；导电装置切割磁感线，电流I2的计算公式为：
I2＝B×L×V×Sinα/R；
α为导电装置与垂直磁感线之间的夹角；
R为导电装置的电阻；
B为上磁块21与下磁块22之间的磁感应强度；
导电装置为金属丝或金属管或金属杆。
需要说明的是，电流的作用可刺激导管周围受损轴突神经细胞，促进受损轴突神经细胞的增殖、分化和生长，加快受损轴突神经的再生与修复。
实施例3
本发明中，设上导轨11以及下导轨12的两端分别为M端和N端；
初始状态下：下滑块32固定设置在下导轨12的M端位置处或N端位置处；
运动状态下，上滑块31沿由M向N，再由N向M的往复运动；下滑块32始终不动；
长度为L的导电装置与铺种细胞的培养皿放置在载物台6的中心位置处；上滑块31的移动速度为V；导电装置切割磁感线，电流I3的计算公式为：
I3＝B×L×V×Sinα/R；
α为导电装置与垂直磁感线之间的夹角；
R为导电装置的电阻；
B为上磁块21与下磁块22之间的磁感应强度；
导电装置为金属丝或金属管或金属杆。
需要说明的是，电流的作用在一定程度上可以刺激导管周围神经细胞的生长，从而使长距离和长时间受损的周围神经受加快再生与修复，可以对神经细胞体外培养，促进神经细胞增殖、分化和生长。
综上，本发明具有以下优点：
本发明，通过电刺激更好地促进多种细胞，如肌腱、韧带、神经、血管、皮肤、肌肉和消化道细胞等，尤其是神经细胞的增殖、分化和生长，从而可以促进神经修复和再生，达到更理想的效果；
本发明，装置结构简单，生产和维修保养成本低，易于工业化推广；
本发明，通过程序设定上磁块21和下磁块22的运动轨迹以改变磁场，并实现细胞培养过程的自动化控制；
本发明，根据“磁生电”原理，可以维持磁场的稳定变化，可产生稳定的电流，无需使用原电池，无需使用电解液和电极，绿色环保。
应当理解的是，本发明的上述具体实施方式仅仅用于示例性说明或解释本发明的原理，而不构成对本发明的限制。因此，在不偏离本发明的精神和范围的情况下所做的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。此外，本发明所附权利要求旨在涵盖落入所附权利要求范围和边界、或者这种范围和边界的等同形式内的全部变化和修改例。

Claims (6)

  1. 一种带有微电流刺激功能的细胞培养装置，其特征在于，包括箱体(4)、上导轨(11)、上磁块(21)、上滑块(31)、下导轨(12)、下磁块(22)、下滑块(32)和载物台(6)；
    箱体(4)为医用级聚合物箱体或不锈钢箱体；箱体(4)包括培养箱(41)和控制箱(42)；培养箱(41)和控制箱(42)并排设置；
    上导轨(11)、上磁块(21)、上滑块(31)、下导轨(12)、下磁块(22)、下滑块(32)和载物台(6)均设置在培养箱(41)内；载物台(6)位于上导轨(11)与下导轨(12)的中间位置处；载物台(6)为水平设置；
    上滑块(31)滑动设置在上导轨(11)上，上磁块(21)设置在上滑块(31)的下表面；下滑块(32)滑动设置在下导轨(12)上，下磁块(22)设置在下滑块(32)的上表面；上磁块(21)与下磁块(22)朝向载物台(6)的一面，上磁块(21)与下磁块(22)磁极相反；
    控制箱(42)内部设有用于控制上滑块(31)及下磁块(22)滑动的控制装置。
  2. 根据权利要求1所述的带有微电流刺激功能的细胞培养装置，其特征在于，载物台(6)距离上导轨(11)以及下导轨(12)之间的距离为1-50cm；
    上磁块(21)底面与载物台(6)之间的距离为X；下磁块(22)上表面与载物台(6)之间的距离为X；X为0.5-50cm。
  3. 根据权利要求1所述的带有微电流刺激功能的细胞培养装置，其特征在于，上滑块(31)以及下滑块(32)的滑动速度为1-100cm/s。
  4. 根据权利要求1-3任一项所述的带有微电流刺激功能的细胞培养装置，其特征在于，设上导轨(11)以及下导轨(12)的两端分别为M端和N端；
    初始状态下：上滑块(31)滑动设置在上导轨(11)的M端位置处，下滑块(32)滑动设置在下导轨(12)的M端位置处；
    运动状态下，上滑块(31)与下滑块(32)同步移动，沿由M向N，再由N向M的往复运动；上滑块(31)与下滑块(32)移动方向和移动速度始终相同；
    长度为L的导电装置与铺种细胞的培养皿放置在载物台(6)的中心位置处；
    上滑块(31)及下滑块(32)的移动速度为V；导电装置切割磁感线，电流I1的计算公式为：
    I1＝(B×L×V×Sinα)/R；
    α为导电装置与垂直磁感线之间的夹角；
    R为导电装置的电阻；
    B为上磁块(21)与下磁块(22)之间的磁感应强度；
    导电装置为金属丝或金属管或金属杆。
  5. 根据权利要求1-3任一项所述的带有微电流刺激功能的细胞培养装置，其特征在于，设上导轨(11)以及下导轨(12)的两端分别为M端和N端；
    初始状态下：上滑块(31)滑动设置在上导轨(11)的M端位置处，下滑块(32)滑动设置在下导轨(12)的N端位置处；
    运动状态下，上滑块(31)沿由M向N，再由N向M的往复运动；下滑块(32)沿由N向M，再由M向N的往复运动；
    上滑块(31)与下滑块(32)移动方向始终相反，移动速度始终相同；
    长度为L的导电装置与铺种细胞的培养皿放置在载物台(6)的中心位置处；
    上滑块(31)及下滑块(32)的移动速度为V；导电装置切割磁感线，电流I2的计算公式为：
    I2＝(B×L×V×Sinα)/R；
    α为导电装置与垂直磁感线之间的夹角；
    R为导电装置的电阻；
    B为上磁块(21)与下磁块(22)之间的磁感应强度；
    导电装置为金属丝或金属管或金属杆。
  6. 根据权利要求1-3任一项所述的带有微电流刺激功能的细胞培养装置，其特征在于，设上导轨(11)以及下导轨(12)的两端分别为M端和N端；
    初始状态下：下滑块(32)固定设置在下导轨(12)的M端位置处或N端位置处；
    运动状态下，上滑块(31)沿由M向N，再由N向M的往复运动；下滑块(32)始终不动；
    长度为L的导电装置与铺种细胞的培养皿放置在载物台(6)的中心位置处；上滑块(31)的移动速度为V；导电装置切割磁感线，电流I3的计算公式为：
    I3＝(B×L×V×Sinα)/R；
    α为导电装置与垂直磁感线之间的夹角；
    R为导电装置的电阻；
    B为上磁块(21)与下磁块(22)之间的磁感应强度；
    导电装置为金属丝或金属管或金属杆。
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