WO2022027398A1 - Dispositif d'isolation et de culture de micro-organismes - Google Patents

Dispositif d'isolation et de culture de micro-organismes Download PDF

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Publication number
WO2022027398A1
WO2022027398A1 PCT/CN2020/107273 CN2020107273W WO2022027398A1 WO 2022027398 A1 WO2022027398 A1 WO 2022027398A1 CN 2020107273 W CN2020107273 W CN 2020107273W WO 2022027398 A1 WO2022027398 A1 WO 2022027398A1
Authority
WO
WIPO (PCT)
Prior art keywords
incubator
rotating
bearing
rotating rod
rod
Prior art date
Application number
PCT/CN2020/107273
Other languages
English (en)
Chinese (zh)
Inventor
郑娉婷
Original Assignee
南京溧水高新创业投资管理有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 南京溧水高新创业投资管理有限公司 filed Critical 南京溧水高新创业投资管理有限公司
Priority to PCT/CN2020/107273 priority Critical patent/WO2022027398A1/fr
Publication of WO2022027398A1 publication Critical patent/WO2022027398A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus

Definitions

  • the invention relates to the field of culture devices, in particular to a microorganism separation and culture device.
  • the process of simulating the ecological environment of microorganisms to separate and purify microorganisms with different media and culture conditions At present, only a small number of microorganisms in nature can be isolated and cultivated, which seriously hinders the development of microbial life.
  • the research of activity law and the development of microbial resources the cultivation of microorganisms is mainly through the cultivation equipment and medium, the medium is the nutrient matrix required by the microorganisms, and the traditional medium is static and non-flowing in the cultivation equipment.
  • the medium is static, the nutrients cannot be transferred to each other, and it is easy to cause some microorganisms to lack nutrients and cannot grow well.
  • the main purpose of the present invention is to provide a device for separating and culturing microorganisms, which can effectively solve the problems in the background technology.
  • a microorganism separating and culturing device comprising an incubator, a No. 1 rotating rod is arranged in the middle of the incubator, a No. 1 bearing is arranged at the bottom of the incubator corresponding to the position of the No. 1 rotating rod, and the bottom of the No. 1 rotating rod is movable It is installed in the No. 1 bearing, and the upper end of the No. 1 rotating rod is also provided with a No. 1 bearing.
  • the outer side of the No. 1 bearing on the upper end of the No. 1 rotating rod is provided with a side connecting plate, and the outer side of the side connecting plate is connected to the incubator.
  • the inner wall is fixedly installed, the middle position of the No.
  • the rotating rod is provided with a connecting gear
  • the lower part of the incubator is provided with a screw rod corresponding to the position of the connecting gear
  • the outer end of the screw rod is provided with a side rotating wheel.
  • the rotating rod is provided with a placing plate at the position of the upper No. 1 bearing, the placing plate is provided with several culture dishes, the upper end of the No. 1 rotating rod is provided with a screw rod, and a No. 1 rotating ring is set on the screw rod, so
  • the outer side of the No. 1 rotating ring is provided with several No. 1 connecting rods, the outer side of the No. 1 connecting rod is provided with an outer rotating ring, the bottom of the outer rotating ring is provided with a lower rubber ring, and the screw rod is located on a
  • the No. 2 rotating circle is arranged above the No. 2 rotating circle, and the upper end of the incubator is provided with a cover.
  • No. 2 bearings are provided on the left and right sides of the corresponding screw rod on the incubator, and the screw rod is movably installed in the No. 2 bearing.
  • a side handle is provided on the outer side of the side rotating wheel.
  • the outer edge of the placing tray is provided with an outer baffle
  • the outer bottom of the placing tray is provided with a bottom connecting body
  • the bottom connecting body is provided with rolling steel balls
  • the side connecting plate corresponds to the position of the rolling steel balls A rolling groove is provided.
  • the No. 2 rotating ring is also provided with several rotating handles.
  • the cover and the incubator are movably installed through hinges.
  • the present invention has the following beneficial effects.
  • the petri dish is placed on the placing tray, the No. 1 rotating ring is movably installed on the screw, and the No. 1 rotating ring is rotated downwards.
  • the lower rubber ring of the outer rotating ring on the outer side is stuck on the top of the petri dish.
  • the side handle is intermittently and regularly rotated slowly. The medium flows, so that nutrients are fully transferred to each other, so that the microorganisms can grow better.
  • FIG. 1 is a schematic diagram of the overall structure of a device for separating and culturing microorganisms according to the present invention.
  • FIG. 2 is a schematic structural diagram of the No. 1 rotating circle of a microorganism separating and culturing device of the present invention.
  • FIG. 3 is a schematic structural diagram of the No. 2 rotating circle of a microorganism separating and culturing device of the present invention.
  • connection may be a fixed connection It can also be a detachable connection or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or the internal communication between the two components.
  • connected may be a fixed connection It can also be a detachable connection or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or the internal communication between the two components.
  • a microorganism separation and culture device includes an incubator 1, a No. 1 rotating rod 2 is arranged in the middle of the incubator 1, and a No. 1 bearing 3 is arranged at the bottom of the incubator 1 corresponding to the position of the No. 1 rotating rod 2 , the bottom of the No. 1 rotating rod 2 is movably installed in the No. 1 bearing 3, and the upper end of the No. 1 rotating rod 2 is also provided with a No. 1 bearing 3, and the outer side of the No. 1 bearing 3 at the upper end of the No. 1 rotating rod 2 is provided with a side connecting plate 4.
  • the outer side of the side connecting plate 4 and the inner wall of the incubator 1 are fixedly installed, the middle position of the No.
  • the No. 1 rotating rod 2 is provided with a connecting gear 5, and the lower part of the incubator 1 is provided with a screw rod 7 corresponding to the position of the connecting gear 5, and the screw rod 7
  • the outer side end of the shaft is provided with a side turning wheel 8
  • the No. 1 turning rod 2 is provided with a placing plate 10 at the position of the upper No. 1 bearing 3
  • the placing plate 10 is provided with a number of culture dishes 12, and the upper end of the No. 1 turning rod 2 is provided with
  • There is a screw 16 a No. 1 rotating ring 17 is arranged on the screw 16, a number of No. 1 connecting rods 18 are arranged on the outside of the No.
  • the bottom is provided with a lower rubber ring 20
  • the screw 16 is provided with a second rotation ring 21 above the first rotation ring 17
  • the upper end of the incubator 1 is provided with a cover 23 .
  • the incubator 1 in order to facilitate the rotation of the screw rod 7 , the incubator 1 is provided with No. 2 bearings 6 corresponding to the left and right sides of the screw rod 7 , and the screw rod 7 is movably installed in the No. 2 bearing 6 .
  • a side handle 9 is provided on the outer side of the side turning wheel 8 .
  • the outer edge of the placing tray 10 is provided with an outer blocking body 11
  • the outer bottom of the placing tray 10 is provided with a bottom connecting body 13
  • the bottom connecting body 13 is provided with a bottom connecting body 13.
  • Rolling steel balls 14 are provided, and rolling grooves 15 are provided on the side connecting plate 4 at positions corresponding to the rolling steel balls 14 .
  • a plurality of rotating handles 22 are also provided on the No. 2 rotating ring 21 .
  • cover 23 and the incubator 1 are movably installed through hinges 24, which facilitates the installation of the cover 23.
  • the present invention is a device for separating and cultivating microorganisms. After placing the microorganisms in the petri dish 12, the petri dish 12 is placed on the placing plate 10 in turn, and then the No. 1 rotating circle 17 passes through the The thread and the screw 16 are movably installed with each other, and the No. 1 rotating ring 17 is rotated through several No. 1 connecting rods 18 on the No. 1 rotating ring 17 to move it downward, so that the outer side of the No. 1 rotating ring 17 is at the bottom of the outer rotating ring 19. The lower rubber ring 20 is clamped on the petri dish 12, thereby fixing the petri dish 12, and then the No.
  • the side handle 9 is regularly rotated after a period of time. , so that the side rotating wheel 8 rotates, so that the screw rod 7 rotates in the No. 2 bearing 6, and through the structure of the screw rod 7 and the connecting gear 5 meshing with each other, the connecting gear 5 rotates to drive the rotation of the No. 1 rotating rod 2 , so that the placing plate 10 above the No.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif d'isolement et de culture de micro-organismes, comprenant un incubateur (1). Une première tige rotative (2) est disposé au milieu de l'incubateur (1) ; un premier palier (3) est disposé au fond de l'incubateur (1) à la position correspondant à la première tige rotative ; le fond de la première tige rotative (2) est monté mobile dans le premier palier (3) ; l'autre premier palier (3) est en outre agencé au niveau de l'extrémité supérieure de la première tige rotative (2) ; une plaque de raccordement latérale (4) est disposé sur le côté extérieur du premier palier (3) située à l'extrémité supérieure de la première tige rotative (2) ; le côté extérieur de la plaque de raccordement latérale (4) et la paroi interne de l'incubateur (1) sont montés fixes ; et un engrenage de liaison (5) est disposé au milieu de la première tige rotative (2). Le dispositif d'isolement et de culture de micro-organismes appartient au domaine de la culture biologique et, au moyen de structures telles qu'un disque de placement (10), peut faire tourner lentement des récipients de culture (12) placés sur le dispositif, permettant ainsi à un milieu de culture dans les récipients de culture de s'écouler et aux substances nutritives d'être transmises plus uniformément et permettant ainsi aux micro-organismes de mieux se développer.
PCT/CN2020/107273 2020-08-06 2020-08-06 Dispositif d'isolation et de culture de micro-organismes WO2022027398A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2020/107273 WO2022027398A1 (fr) 2020-08-06 2020-08-06 Dispositif d'isolation et de culture de micro-organismes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2020/107273 WO2022027398A1 (fr) 2020-08-06 2020-08-06 Dispositif d'isolation et de culture de micro-organismes

Publications (1)

Publication Number Publication Date
WO2022027398A1 true WO2022027398A1 (fr) 2022-02-10

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PCT/CN2020/107273 WO2022027398A1 (fr) 2020-08-06 2020-08-06 Dispositif d'isolation et de culture de micro-organismes

Country Status (1)

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WO (1) WO2022027398A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116004375A (zh) * 2023-02-21 2023-04-25 威海德生技术检测有限公司 一种海洋食品中微生物含量的检测装置及方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060141609A1 (en) * 2004-12-28 2006-06-29 Olympus Corporation Culture observation apparatus, sample tray heat-insulating device and lid
CN202989146U (zh) * 2012-08-14 2013-06-12 中国人民解放军南京军区南京总医院 一种转动式微动态培养箱
CN104169413A (zh) * 2012-02-29 2014-11-26 株式会社Jtec 具有培养液更换功能的细胞培养装置
JP2016013059A (ja) * 2014-06-30 2016-01-28 澁谷工業株式会社 自動培養操作装置
CN209403135U (zh) * 2018-12-31 2019-09-20 内蒙古蒙森农业科技股份有限公司 一种马铃薯种薯繁育用培养箱
CN210163449U (zh) * 2019-05-15 2020-03-20 湖北省肿瘤医院（湖北省肿瘤研究所） 一种药品检测用微生物定量培养基台

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060141609A1 (en) * 2004-12-28 2006-06-29 Olympus Corporation Culture observation apparatus, sample tray heat-insulating device and lid
CN104169413A (zh) * 2012-02-29 2014-11-26 株式会社Jtec 具有培养液更换功能的细胞培养装置
CN202989146U (zh) * 2012-08-14 2013-06-12 中国人民解放军南京军区南京总医院 一种转动式微动态培养箱
JP2016013059A (ja) * 2014-06-30 2016-01-28 澁谷工業株式会社 自動培養操作装置
CN209403135U (zh) * 2018-12-31 2019-09-20 内蒙古蒙森农业科技股份有限公司 一种马铃薯种薯繁育用培养箱
CN210163449U (zh) * 2019-05-15 2020-03-20 湖北省肿瘤医院（湖北省肿瘤研究所） 一种药品检测用微生物定量培养基台

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116004375A (zh) * 2023-02-21 2023-04-25 威海德生技术检测有限公司 一种海洋食品中微生物含量的检测装置及方法

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