WO2022009134A3 - Method of encapsulation of an active protein using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and its use for the production of a veterinary composition for the treatment of atopic dermatitis in animals - Google Patents
Method of encapsulation of an active protein using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and its use for the production of a veterinary composition for the treatment of atopic dermatitis in animals Download PDFInfo
- Publication number
- WO2022009134A3 WO2022009134A3 PCT/IB2021/056127 IB2021056127W WO2022009134A3 WO 2022009134 A3 WO2022009134 A3 WO 2022009134A3 IB 2021056127 W IB2021056127 W IB 2021056127W WO 2022009134 A3 WO2022009134 A3 WO 2022009134A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- active protein
- polymer
- composition
- culture
- cells
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
- A61J3/00—Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms
- A61J3/07—Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms into the form of capsules or similar small containers for oral use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/11—Encapsulated compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8129—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal or ketal radical; Compositions of hydrolysed polymers or esters of unsaturated alcohols with saturated carboxylic acids; Compositions of derivatives of such polymers, e.g. polyvinylmethylether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
Abstract
The subject of invention is a method of encapsulating an active protein using electrodeposition techniques, characterised in that it comprises the following steps: (a) establishing a primary mesenchymal cell culture containing 2,000-5,000 source tissue cells and a serum-supplemented culture medium; (b) maintaining the cell culture established in step (a) for 280-340 hours until the culture surface is fully covered by the cultured cells; (c) obtaining a culture fluid from the above of the cultured cells; (d) purifying the culture fluid obtained in step (c) from cell debris and suspended cells by centrifuging said fluid with a force of 300 to 1200 x g; (e) transferring the upper liquid phase from above the sediment to a new vessel; (f) gently mixing the purified liquid phase obtained in step (e) with an aqueous solution of polyvinyl alcohol; (g) adding ethyl alcohol to the mixture obtained in step (f) while stirring continuously; (h) the material obtained in step (g) is deposited on the collector surface by means of electro spinning or electrospraying. Another subject of invention is an immunomodulating composition containing an active protein and a polymer, characterised in that it contains ethyl alcohol, wherein the active protein is a fibrous, fully water-soluble material containing proteins released by mesenchymal cells, including CCL2 at an amount from 0.56 to 5.62 ng/g of dry weight of the composition, and the polymer is an aqueous solution of polyvinyl alcohol. Another subject of invention is an use of the composition according to invention for the preparation of a veterinary composition for the treatment of atopic dermatitis in animals.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL434622A PL434622A1 (en) | 2020-07-10 | 2020-07-10 | Active protein encapsulation method using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and use thereof for preparation of a veterinary composition for treatment of atopic dermatitis in animals |
PL434622 | 2020-07-10 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2022009134A2 WO2022009134A2 (en) | 2022-01-13 |
WO2022009134A3 true WO2022009134A3 (en) | 2022-04-21 |
WO2022009134A4 WO2022009134A4 (en) | 2022-06-16 |
Family
ID=77543543
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2021/056127 WO2022009134A2 (en) | 2020-07-10 | 2021-07-08 | Method of encapsulation of an active protein using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and its use for the production of a veterinary composition for the treatment of atopic dermatitis in animals |
Country Status (2)
Country | Link |
---|---|
PL (1) | PL434622A1 (en) |
WO (1) | WO2022009134A2 (en) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008020815A1 (en) * | 2006-08-15 | 2008-02-21 | Agency For Science, Technology And Research | Mesenchymal stem cell conditioned medium |
US20120276215A1 (en) * | 2011-04-26 | 2012-11-01 | Riordan Neil H | Therapeutic Conditioned Media |
CN106823009A (en) * | 2017-01-12 | 2017-06-13 | 广东泰宝医疗器械技术研究院有限公司 | A kind of antibacterial bone renovating bracket material with induction repair and preparation method thereof |
CN109172606A (en) * | 2018-11-30 | 2019-01-11 | 天津欣普赛尔生物医药科技有限公司 | A kind of sustained-release micro-spheres and preparation method of the body of excretion containing mescenchymal stem cell |
WO2019231562A1 (en) * | 2018-05-30 | 2019-12-05 | Direct Biologics Llc | A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use |
WO2019238952A1 (en) * | 2018-06-15 | 2019-12-19 | Marinas Pardo Luis | Pharmaceutical composition for dermatology and uses thereof |
WO2019246014A1 (en) * | 2018-06-18 | 2019-12-26 | North Carolina State University | Stem cell biomimetic nanoparticle therapeutic agents and uses thereof |
-
2020
- 2020-07-10 PL PL434622A patent/PL434622A1/en unknown
-
2021
- 2021-07-08 WO PCT/IB2021/056127 patent/WO2022009134A2/en active Application Filing
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
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US9029146B2 (en) * | 2005-09-02 | 2015-05-12 | Agency For Science, Technology And Research | Mesenchymal stem cell conditioned medium |
WO2008020815A1 (en) * | 2006-08-15 | 2008-02-21 | Agency For Science, Technology And Research | Mesenchymal stem cell conditioned medium |
US20120276215A1 (en) * | 2011-04-26 | 2012-11-01 | Riordan Neil H | Therapeutic Conditioned Media |
CN106823009A (en) * | 2017-01-12 | 2017-06-13 | 广东泰宝医疗器械技术研究院有限公司 | A kind of antibacterial bone renovating bracket material with induction repair and preparation method thereof |
WO2019231562A1 (en) * | 2018-05-30 | 2019-12-05 | Direct Biologics Llc | A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use |
WO2019238952A1 (en) * | 2018-06-15 | 2019-12-19 | Marinas Pardo Luis | Pharmaceutical composition for dermatology and uses thereof |
WO2019246014A1 (en) * | 2018-06-18 | 2019-12-26 | North Carolina State University | Stem cell biomimetic nanoparticle therapeutic agents and uses thereof |
CN109172606A (en) * | 2018-11-30 | 2019-01-11 | 天津欣普赛尔生物医药科技有限公司 | A kind of sustained-release micro-spheres and preparation method of the body of excretion containing mescenchymal stem cell |
Non-Patent Citations (9)
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CHO B. ET AL: "The effect of exosome from human adipose tissue-derived mesenchymal stem cells on atopic dermatitis in the house dust mite antigen-induced mouse model", CYTOTHERAPY, vol. 20, no. 5, 1 May 2018 (2018-05-01), GB, pages S23 - S24, XP055777638, ISSN: 1465-3249, DOI: 10.1016/j.jcyt.2018.02.055 * |
CHO BYONG SEUND ET AL: "ASC-EXOSOME as a potential therapeutic for atopic dermatitis", ISEV2019 ABSTRACT BOOK IN JOURNAL OF EXTRACELLULAR VESICLES, vol. 8, 28 February 2019 (2019-02-28), pages 1 - 394, XP055887183, Retrieved from the Internet <URL:https://www.tandfonline.com/doi/pdf/10.1080/20013078.2019.1593587> DOI: 10.1080/20013078.2019.1593587 * |
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Also Published As
Publication number | Publication date |
---|---|
PL434622A1 (en) | 2022-01-17 |
WO2022009134A4 (en) | 2022-06-16 |
WO2022009134A2 (en) | 2022-01-13 |
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