PL434622A1 - Active protein encapsulation method using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and use thereof for preparation of a veterinary composition for treatment of atopic dermatitis in animals - Google Patents

Active protein encapsulation method using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and use thereof for preparation of a veterinary composition for treatment of atopic dermatitis in animals

Info

Publication number
PL434622A1
PL434622A1 PL434622A PL43462220A PL434622A1 PL 434622 A1 PL434622 A1 PL 434622A1 PL 434622 A PL434622 A PL 434622A PL 43462220 A PL43462220 A PL 43462220A PL 434622 A1 PL434622 A1 PL 434622A1
Authority
PL
Poland
Prior art keywords
active protein
polymer
composition
cells
culture
Prior art date
Application number
PL434622A
Other languages
Polish (pl)
Inventor
Łukasz Bzdzion
Jakub GRZESIAK
Karol Wrzeszcz
Original Assignee
Bioceltix Spółka Akcyjna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioceltix Spółka Akcyjna filed Critical Bioceltix Spółka Akcyjna
Priority to PL434622A priority Critical patent/PL434622A1/en
Priority to PCT/IB2021/056127 priority patent/WO2022009134A2/en
Publication of PL434622A1 publication Critical patent/PL434622A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J3/00—Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms
    • A61J3/07—Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms into the form of capsules or similar small containers for oral use
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/11—Encapsulated compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
    • A61K8/8129—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal or ketal radical; Compositions of hydrolysed polymers or esters of unsaturated alcohols with saturated carboxylic acids; Compositions of derivatives of such polymers, e.g. polyvinylmethylether
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning

Abstract

Przedmiotem wynalazku jest sposób enkapsulacji białkowej substancji czynnej wykorzystujący techniki elektroosadzania, charakteryzujący się tym, że obejmuje następujące etapy: (a) założenie pierwotnej hodowli komórek mezenchymalnych zawierającej 2000-5000 komórek tkanki źródłowej oraz podłoże hodowlane suplementowane surowicą; (b) prowadzenie hodowli komórkowej założonej w etapie (a) przez 280-340 godzin do osiągnięcia pełnego pokrycia powierzchni hodowlanej przez hodowane komórki; (c) pozyskanie płynu hodowlanego z nad hodowanych komórek; (d) oczyszczenie płynu hodowlanego pozyskanego w etapie (c) z pozostałości komórkowych oraz zawieszonych komórek poprzez odwirowanie wspomnianego płynu z siłą od 300 do 1200 x g; (e) przeniesienie górnej fazy płynnej z nad osadu do nowego naczynia; (f) delikatne wymieszanie oczyszczonej fazy płynnej uzyskanej w etapie (e) z roztworem wodnym alkoholu poliwinylowego; (g) dodanie alkoholu etylowego do mieszaniny uzyskanej w etapie (f) przy ciągłym mieszaniu; (h) materiał uzyskany w etapie (g) jest deponowany na powierzchni kolektora za pomocą elektroprzędzenia albo elektrorozpylania. Kolejnym przedmiotem wynalazku jest immunomodulująca kompozycja zawierająca białkową substancję czynną oraz polimer, charakteryzująca się tym, że zawiera alkohol etylowy, białkową substancję czynną stanowi włóknisty, w pełni rozpuszczalny w wodzie materiał zawierający białka uwalniane przez komórki mezenchymalne, w tym CCL2 w ilości od 0,56 do 5,62 ng/g suchej masy kompozycji, natomiast polimer stanowi roztwór wodny alkoholu poliwinylowego. Kolejnym przedmiotem wynalazku jest zastosowanie kompozycji według wynalazku do wytwarzania kompozycji weterynaryjnej do leczenia atopowego zapalenia skóry u zwierząt.The invention relates to an active protein encapsulation method using electrodeposition techniques, characterized in that it comprises the following steps: (a) establishing a primary mesenchymal cell culture containing 2,000-5,000 cells of the source tissue and a serum-supplemented culture medium; (b) carrying out the cell culture established in step (a) for 280-340 hours until the culture area is fully covered by the cultured cells; (c) obtaining a culture fluid from the super cultured cells; (d) purifying the culture fluid obtained in step (c) from cell debris and suspended cells by centrifuging said fluid with a force of 300 to 1200 x g; (e) transferring the upper liquid phase from above the sediment to a new vessel; (f) gently mixing the purified liquid phase obtained in step (e) with an aqueous solution of the polyvinyl alcohol; (g) adding ethyl alcohol to the mixture obtained in step (f) with continuous stirring; (h) the material obtained from step (g) is deposited on the surface of the collector by means of electrospinning or electrospray. Another object of the invention is an immunomodulatory composition containing a protein active substance and a polymer, characterized in that it contains ethyl alcohol, the protein active substance is a fibrous, fully water-soluble material containing proteins released by mesenchymal cells, including CCL2 in an amount from 0.56 to 5.62 ng / g dry weight of the composition, while the polymer is an aqueous solution of polyvinyl alcohol. A further object of the invention is the use of a composition according to the invention for the preparation of a veterinary composition for the treatment of atopic dermatitis in animals.

PL434622A 2020-07-10 2020-07-10 Active protein encapsulation method using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and use thereof for preparation of a veterinary composition for treatment of atopic dermatitis in animals PL434622A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PL434622A PL434622A1 (en) 2020-07-10 2020-07-10 Active protein encapsulation method using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and use thereof for preparation of a veterinary composition for treatment of atopic dermatitis in animals
PCT/IB2021/056127 WO2022009134A2 (en) 2020-07-10 2021-07-08 Method of encapsulation of an active protein using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and its use for the production of a veterinary composition for the treatment of atopic dermatitis in animals

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL434622A PL434622A1 (en) 2020-07-10 2020-07-10 Active protein encapsulation method using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and use thereof for preparation of a veterinary composition for treatment of atopic dermatitis in animals

Publications (1)

Publication Number Publication Date
PL434622A1 true PL434622A1 (en) 2022-01-17

Family

ID=77543543

Family Applications (1)

Application Number Title Priority Date Filing Date
PL434622A PL434622A1 (en) 2020-07-10 2020-07-10 Active protein encapsulation method using electrodeposition techniques, an immunomodulating composition containing the active protein and a polymer, and use thereof for preparation of a veterinary composition for treatment of atopic dermatitis in animals

Country Status (2)

Country Link
PL (1) PL434622A1 (en)
WO (1) WO2022009134A2 (en)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2054506A1 (en) * 2006-08-15 2009-05-06 Agency for Science, Technology and Research Mesenchymal stem cell conditioned medium
US20120276215A1 (en) * 2011-04-26 2012-11-01 Riordan Neil H Therapeutic Conditioned Media
CN106823009A (en) * 2017-01-12 2017-06-13 广东泰宝医疗器械技术研究院有限公司 A kind of antibacterial bone renovating bracket material with induction repair and preparation method thereof
KR20210042268A (en) * 2018-05-30 2021-04-19 다이렉트 바이오로직스 엘엘씨 Growth factor and extracellular vesicle freezing or powdering additive including mesenchymal stem cell (MSC) preparation and method of use
EP3806872A1 (en) * 2018-06-15 2021-04-21 Mariñas Pardo, Luis Pharmaceutical composition for dermatology and uses thereof
WO2019246014A1 (en) * 2018-06-18 2019-12-26 North Carolina State University Stem cell biomimetic nanoparticle therapeutic agents and uses thereof
CN109172606A (en) * 2018-11-30 2019-01-11 天津欣普赛尔生物医药科技有限公司 A kind of sustained-release micro-spheres and preparation method of the body of excretion containing mescenchymal stem cell

Also Published As

Publication number Publication date
WO2022009134A4 (en) 2022-06-16
WO2022009134A3 (en) 2022-04-21
WO2022009134A2 (en) 2022-01-13

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