WO2022003130A3 - Système d'expression de levure - Google Patents
Système d'expression de levure Download PDFInfo
- Publication number
- WO2022003130A3 WO2022003130A3 PCT/EP2021/068252 EP2021068252W WO2022003130A3 WO 2022003130 A3 WO2022003130 A3 WO 2022003130A3 EP 2021068252 W EP2021068252 W EP 2021068252W WO 2022003130 A3 WO2022003130 A3 WO 2022003130A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- expression system
- yeast expression
- expression
- saccharomyces
- enzymes
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01084—Alcohol O-acetyltransferase (2.3.1.84)
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne l'expression d'enzymes hétérologues sous le contrôle de promoteurs constitutifs, en particulier l'expression d'enzymes de type acétyltransférase chez un hôte sélectionné à partir de Saccharomyces.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH00815/20 | 2020-07-01 | ||
CH8152020 | 2020-07-01 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2022003130A2 WO2022003130A2 (fr) | 2022-01-06 |
WO2022003130A3 true WO2022003130A3 (fr) | 2022-03-17 |
Family
ID=76807653
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2021/068252 WO2022003130A2 (fr) | 2020-07-01 | 2021-07-01 | Système d'expression de levure |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2022003130A2 (fr) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014096992A1 (fr) * | 2012-12-20 | 2014-06-26 | Dsm Ip Assets B.V. | Acétyltransférases et leur utilisation pour la production de caroténoïdes |
WO2019058001A1 (fr) * | 2017-09-25 | 2019-03-28 | Dsm Ip Assets B.V. | Production d'esters de rétinyle |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2371967B1 (fr) | 2005-03-18 | 2015-06-03 | DSM IP Assets B.V. | Production de caroténoïdes dans des levures oléagineuses et des champignons |
EP2078092A2 (fr) | 2006-09-28 | 2009-07-15 | Microbia, Inc. | Production de caroténoïdes dans des levures et des champignons oléagineux |
EP3242949B1 (fr) | 2015-01-06 | 2021-11-03 | DSM IP Assets B.V. | Système crispr-cas destiné à une cellule hôte de lévure |
WO2016172282A1 (fr) | 2015-04-21 | 2016-10-27 | Dsm Ip Assets B.V. | Production microbienne de terpénoïdes |
-
2021
- 2021-07-01 WO PCT/EP2021/068252 patent/WO2022003130A2/fr active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014096992A1 (fr) * | 2012-12-20 | 2014-06-26 | Dsm Ip Assets B.V. | Acétyltransférases et leur utilisation pour la production de caroténoïdes |
WO2019058001A1 (fr) * | 2017-09-25 | 2019-03-28 | Dsm Ip Assets B.V. | Production d'esters de rétinyle |
Non-Patent Citations (5)
Title |
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"Lachancea mirantina acetyl transferase 1 (LmATF1), SEQ:13", GENESEQ,, 16 May 2019 (2019-05-16), XP002794500 * |
HONG SEUNG-HYE ET AL: "Biochemical properties of retinoid-converting enzymes and biotechnological production of retinoids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 99, no. 19, 1 August 2015 (2015-08-01), pages 7813 - 7826, XP035541982, ISSN: 0175-7598, [retrieved on 20150801], DOI: 10.1007/S00253-015-6830-8 * |
HUI-JEONG JANG ET AL: "Selective retinol production by modulating the composition of retinoids from metabolically engineered E. coli", BIOTECHNOLOGY AND BIOENGINEERING, WILEY, US, vol. 112, no. 8, 12 May 2015 (2015-05-12), pages 1604 - 1612, XP071101922, ISSN: 0006-3592, DOI: 10.1002/BIT.25577 * |
LACHANCEA FERMENTATI -: "Hit details for GSP:BGE36667", 28 March 2019 (2019-03-28), XP055853908, Retrieved from the Internet <URL:http://ibis.internal.epo.org/exam/hitDetails.jsp?id=303123902> [retrieved on 20211022] * |
SACCHAROMYCES ARBORICOLA: "Hit details for EPOP:JC582901", 10 September 2014 (2014-09-10), XP055853926, Retrieved from the Internet <URL:Hit details for EPOP:JC582901> [retrieved on 20211022] * |
Also Published As
Publication number | Publication date |
---|---|
WO2022003130A2 (fr) | 2022-01-06 |
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