WO2022003130A3 - Système d'expression de levure - Google Patents

Système d'expression de levure Download PDF

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Publication number
WO2022003130A3
WO2022003130A3 PCT/EP2021/068252 EP2021068252W WO2022003130A3 WO 2022003130 A3 WO2022003130 A3 WO 2022003130A3 EP 2021068252 W EP2021068252 W EP 2021068252W WO 2022003130 A3 WO2022003130 A3 WO 2022003130A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression system
yeast expression
expression
saccharomyces
enzymes
Prior art date
Application number
PCT/EP2021/068252
Other languages
English (en)
Other versions
WO2022003130A2 (fr
Inventor
Valmik Kanubhai VYAS
Peter Louis HOUSTON
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2022003130A2 publication Critical patent/WO2022003130A2/fr
Publication of WO2022003130A3 publication Critical patent/WO2022003130A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01084—Alcohol O-acetyltransferase (2.3.1.84)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne l'expression d'enzymes hétérologues sous le contrôle de promoteurs constitutifs, en particulier l'expression d'enzymes de type acétyltransférase chez un hôte sélectionné à partir de Saccharomyces.
PCT/EP2021/068252 2020-07-01 2021-07-01 Système d'expression de levure WO2022003130A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH8152020 2020-07-01
CH00815/20 2020-07-01

Publications (2)

Publication Number Publication Date
WO2022003130A2 WO2022003130A2 (fr) 2022-01-06
WO2022003130A3 true WO2022003130A3 (fr) 2022-03-17

Family

ID=76807653

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2021/068252 WO2022003130A2 (fr) 2020-07-01 2021-07-01 Système d'expression de levure

Country Status (1)

Country Link
WO (1) WO2022003130A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014096992A1 (fr) * 2012-12-20 2014-06-26 Dsm Ip Assets B.V. Acétyltransférases et leur utilisation pour la production de caroténoïdes
WO2019058001A1 (fr) * 2017-09-25 2019-03-28 Dsm Ip Assets B.V. Production d'esters de rétinyle

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0609040B1 (pt) 2005-03-18 2018-07-31 Microbia, Inc. Fungo recombinante do gênero yarrowia, método de produção de um carotenóide e método de preparo de um aditivo alimentício ou ração contendo um carotenóide
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
DK3242949T3 (da) 2015-01-06 2022-01-24 Dsm Ip Assets Bv Crispr-cas-system til en gærværtscelle
WO2016170045A1 (fr) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Géranylgéranyle pyrophosphate synthase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014096992A1 (fr) * 2012-12-20 2014-06-26 Dsm Ip Assets B.V. Acétyltransférases et leur utilisation pour la production de caroténoïdes
WO2019058001A1 (fr) * 2017-09-25 2019-03-28 Dsm Ip Assets B.V. Production d'esters de rétinyle

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Lachancea mirantina acetyl transferase 1 (LmATF1), SEQ:13", GENESEQ,, 16 May 2019 (2019-05-16), XP002794500 *
HONG SEUNG-HYE ET AL: "Biochemical properties of retinoid-converting enzymes and biotechnological production of retinoids", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 99, no. 19, 1 August 2015 (2015-08-01), pages 7813 - 7826, XP035541982, ISSN: 0175-7598, [retrieved on 20150801], DOI: 10.1007/S00253-015-6830-8 *
HUI-JEONG JANG ET AL: "Selective retinol production by modulating the composition of retinoids from metabolically engineered E. coli", BIOTECHNOLOGY AND BIOENGINEERING, WILEY, US, vol. 112, no. 8, 12 May 2015 (2015-05-12), pages 1604 - 1612, XP071101922, ISSN: 0006-3592, DOI: 10.1002/BIT.25577 *
LACHANCEA FERMENTATI -: "Hit details for GSP:BGE36667", 28 March 2019 (2019-03-28), XP055853908, Retrieved from the Internet <URL:http://ibis.internal.epo.org/exam/hitDetails.jsp?id=303123902> [retrieved on 20211022] *
SACCHAROMYCES ARBORICOLA: "Hit details for EPOP:JC582901", 10 September 2014 (2014-09-10), XP055853926, Retrieved from the Internet <URL:Hit details for EPOP:JC582901> [retrieved on 20211022] *

Also Published As

Publication number Publication date
WO2022003130A2 (fr) 2022-01-06

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