WO2021228499A1 - Appareil et procédé d'échantillonnage automatique permettant la fourniture automatique d'un échantillon pour la détermination qualitative et/ou quantitative d'un anaylte - Google Patents

Appareil et procédé d'échantillonnage automatique permettant la fourniture automatique d'un échantillon pour la détermination qualitative et/ou quantitative d'un anaylte Download PDF

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Publication number
WO2021228499A1
WO2021228499A1 PCT/EP2021/060101 EP2021060101W WO2021228499A1 WO 2021228499 A1 WO2021228499 A1 WO 2021228499A1 EP 2021060101 W EP2021060101 W EP 2021060101W WO 2021228499 A1 WO2021228499 A1 WO 2021228499A1
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WO
WIPO (PCT)
Prior art keywords
sample
liquid
analyte
control electronics
sampling device
Prior art date
Application number
PCT/EP2021/060101
Other languages
German (de)
English (en)
Inventor
Urs Endress
Detlev Wittmer
Manfred Jagiella
Christoph Rompf
Ralf Steuerwald
Angela Eubisch
Michael Hanko
Peter Lindmüller
Eckhard Sommer
Robert MÖLLER
Christine Marion GRÄFE
Monika Heisterkamp
Original Assignee
Endress+Hauser Conducta Gmbh+Co. Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Endress+Hauser Conducta Gmbh+Co. Kg filed Critical Endress+Hauser Conducta Gmbh+Co. Kg
Priority to US17/998,847 priority Critical patent/US20230194390A1/en
Priority to CN202180033791.6A priority patent/CN115516287A/zh
Priority to EP21720435.3A priority patent/EP4150311A1/fr
Publication of WO2021228499A1 publication Critical patent/WO2021228499A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/18—Devices for withdrawing samples in the liquid or fluent state with provision for splitting samples into portions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/20—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
    • G01N1/2035—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials by deviating part of a fluid stream, e.g. by drawing-off or tapping
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G01N33/1826—Organic contamination in water
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/20—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
    • G01N1/2035—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials by deviating part of a fluid stream, e.g. by drawing-off or tapping
    • G01N2001/2071—Removable sample bottle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons

Definitions

  • the invention relates to an automatic sampling device and a method for the automated provision of a sample for a qualitative and / or quantitative determination of an analyte comprising a biopolymer in a sample liquid, in particular in water or wastewater.
  • the invention also relates to a measuring system for the qualitative or quantitative determination of the analyte, which comprises the automatic sampling device, and a method for the qualitative or quantitative determination of the analyte in the sample liquid.
  • the analyte can be a bioparticle and / or a biopolymer, e.g. DNA, RNA, a protein, a virus, a virus component, a bacterium or a bacterial component, or an anthropogenic trace substance or micropollutant, e.g. a pesticide, drug, personal care product or their breakdown products or Metabolites.
  • a biopolymer e.g. DNA, RNA, a protein, a virus, a virus component, a bacterium or a bacterial component, or an anthropogenic trace substance or micropollutant, e.g. a pesticide, drug, personal care product or their breakdown products or Metabolites.
  • virus components e.g. RNA
  • the laboratory examination usually initially comprises complex preparatory procedures for the concentration and / or extraction of the analyte in order to provide a sample suitable for the qualitative or quantitative analysis.
  • the sample produced in this way is then analyzed using an immunological method or a method based on quantitative real-time PCR (English technical term: real-time quantitative PCR or qPCR for short).
  • This procedure is not suitable for systematic monitoring of wastewater, which could serve as the basis for an early warning system even for extensive geographical areas, e.g. for a large number of municipalities or districts, since the associated time and personnel expenditure is very high.
  • the object of the invention is to provide a device and a method that allow systematic monitoring of water or wastewater for the occurrence of certain analytes, for example biopolymers or bioparticles, such as viruses, virus components, bacteria, bacterial components, proteins, DNA or RNA , or anthropogenic micropollutants.
  • biopolymers or bioparticles such as viruses, virus components, bacteria, bacterial components, proteins, DNA or RNA , or anthropogenic micropollutants.
  • the object is achieved by the automatic sampling device according to claim 1, the measuring system according to claim 12, the method for the automated provision of a sample according to claim 16 and the method for the qualitative or quantitative determination of at least one analyte according to claim 23 specified.
  • the automatic sampling device according to the invention for taking liquid samples from a sample liquid present at a sampling point of a body of water or a container, in particular water or waste water, for the qualitative or quantitative determination of at least one analyte contained in the sample liquid, in particular a biopolymer comprises: one with the sampling point fluidically connectable sample line; a pumping device; at least one sample container that can be fluidically connected to the sample line; and control electronics that are configured to fluidically connect the sample line to the at least one
  • control electronics are further set up to transport a predeterminable volume of the sample liquid as a liquid sample along the fluid flow path into the sample container by means of the pumping device, wherein the sampling device has means for concentrating and / or extracting the analyte present in the liquid sample.
  • a sample prepared for a subsequent quantitative or qualitative determination can be made available with a volume that is significantly reduced compared to the liquid sample originally taken from the sampling point. This makes it easier to transport the sample to be examined to a laboratory for further analysis.
  • the sample obtained by concentration and / or extraction is also advantageously suitable for, in particular automated, transfer to an analysis device.
  • the removal of the liquid sample and the concentration or extraction can advantageously be carried out partially or completely automatically by means of the sampling device. This allows systematic monitoring of the sample liquid for the presence of the analyte at a single sampling point or also at a large number of sampling points in an extensive geographical area.
  • a qualitative determination of the analyte is understood here and below to mean a method in which it is determined whether the analyte is present in the liquid sample.
  • a quantitative determination also includes the determination of a value representing the concentration of the analyte in the liquid sample.
  • the device can be set up to concentrate and / or extract one or more analytes to be determined.
  • the at least one analyte can be a bioparticle or a biopolymer, for example DNA, RNA, a protein, a virus, a virus component, a bacterium or a bacterial component, or an anthropogenic trace substance or micropollutant, e.g. a pesticide, drug, personal care product or its degradation product Metabolite.
  • the sample liquid can be waste water, water present in a body of water or water, in particular drinking water, in a supply network.
  • the aforementioned sampling point can be, for example, a point on or in a body of water.
  • a container containing the sample liquid can be a pipe through which the sample liquid flows, or an open basin or channel.
  • the sampling point can also be arranged on or in a pipeline through which the sample liquid flows, fluidly connected or connectable to the sample line, e.g. in a water supply network or in a sewage network, or on or in a basin, e.g. in a local or communal sewage treatment plant be.
  • the sampling point can be, for example, a point in the area of the inlet of a sewage treatment plant. It can also be arranged at a point in a sewage network, for example in a discharge point or in the area of a discharge point into the sewage network.
  • the sampling device can have a plurality of sample containers, each of which can be individually fluidically connected to the sample line.
  • the sampling device can have a sample distributor fluidically connected to the sample line, e.g. in the form of a movable distributor arm, the position of which can be adjusted by the control electronics in order to fluidically connect one of the sample containers to the sample line by means of the sample distributor.
  • the sample distributor can also be implemented by means of a valve device and fluid lines which can be individually connected to the sample line via the valve device and which lead to the sample containers.
  • a filter device with a filter or a filter cascade can be arranged, which is designed to filter solids from the sample liquid but to let the analyte through.
  • the means for concentration and / or extraction can comprise a collecting matrix which can be brought into contact with the liquid sample and which binds the analyte selectively or essentially selectively.
  • the collecting matrix can be designed, for example, to selectively bind or immobilize a certain class of biopolymers, e.g. certain proteins or RNA or DNA of certain species, such as, for example, RNA from viruses of a certain type, e.g. of a certain order, family or genus. If the analyte is an anthropogenic trace substance, the collecting matrix can be designed to selectively bind or immobilize a certain substance or a certain class of low molecular weight organic substances.
  • the collecting matrix can be arranged in the at least one sample container or in a raw-flow unit fluidly connected to the sample container, in particular a fluid line or a raw-flow cartridge.
  • the through-flow unit can be arranged in the fluid flow path, in particular upstream of the sample container. It can also be arranged in an upper region of the sample container, for example as an insert close to or in an opening of the sample container.
  • the flow through unit can be detachable with the Fluid line and / or the sample container be connected, so that it is possible to remove the permeable unit together with the collecting matrix and the analyte bound to it from the sampling device and from the sample container and to transport it to a laboratory for further analysis.
  • the collecting matrix can be present loose or in a removable insert in the sample container. The collecting matrix with the analyte bound to it can be removed in this case by removing the insert or by emptying the sample container while retaining the collecting matrix.
  • the collecting matrix can be designed to immobilize several different, but in particular structurally and / or chemically similar analytes, e.g. as already mentioned, certain proteins or a certain class of biopolymers.
  • analytes can be determined qualitatively or quantitatively independently of one another, for example by means of qPCR-based methods.
  • the sampling device can have a plurality of flow-through cartridges arranged in the fluid flow path or in the sample container, each of which contains different collection matrices which are each designed to immobilize at least one specific analyte.
  • the sampling device can also have a plurality of sample containers which each contain different collection matrices in such a way that each collection matrix immobilizes a different analyte. Different liquid samples are used to determine different analytes.
  • the collecting matrix advantageously has a large surface.
  • the surface can have a functionalization that serves to selectively bind the analyte, e.g. RNA, DNA or proteins.
  • it can be coated with silica, glass or cross-linked polymers that are modified with suitable functional groups (e.g. negatively charged hydroxyl groups) for selective interaction with the analyte (e.g. positively charged viruses).
  • suitable functional groups e.g. negatively charged hydroxyl groups
  • analyte e.g. positively charged viruses
  • It can also consist entirely of silica, glass, or cross-linked polymers modified to interact selectively with the analyte.
  • the surface can be modified by antibodies that specifically interact with the analyte or similar catcher structures or catcher molecules or recognition elements based on affine interactions.
  • Aptamers, peptide nucleic acids (PNA), molecularly imprinted polymers (MIP) can be used as capture structures or recognition elements.
  • the collecting matrix can, for example, have a large number of surface-functionalized particles (beads or microbeads), for example polymer or magnetic beads, with a surface-functionalized substrate a roughened or three-dimensionally structured surface, a functionalized fleece, for example a functionalized filter fleece, or a chromatographic separation column.
  • surface-functionalized particles for example polymer or magnetic beads
  • a surface-functionalized substrate a roughened or three-dimensionally structured surface
  • a functionalized fleece for example a functionalized filter fleece
  • chromatographic separation column for example chromatographic separation column.
  • the automatic sampling device can be designed to automatically empty and clean the at least one sample container before or after manual or automatic removal of the collecting matrix with the bound analyte, so that the sample container is available for receiving a new liquid sample.
  • the sampling device can further be set up to present a new collecting matrix for selective binding or immobilization of the at least one analyte in the sample container after the sample container has been emptied and cleaned and the collecting matrix loaded with the analyte has been removed.
  • the sampling device can alternatively also be set up to automatically empty the sample container, to elute the analyte retained in the collecting matrix from the collecting matrix and to collect the eluate as a sample made available for further analysis or to output it as a sample to an automatic analysis device connected to the sampling device.
  • the collecting matrix can remain in the sample container. After removal of the eluate and an optional cleaning process, the sample container with the remaining collecting matrix is available again for receiving a new liquid sample and for concentrating and extracting the analyte in this liquid sample by means of the collecting matrix.
  • the means for concentration and / or extraction can comprise a centrifuge integrated into the device.
  • the control electronics can be set up to store an identification of the liquid sample for each liquid sample taken from the extraction point, and to store a time stamp, which indicates the point in time at which the liquid sample is taken from the extraction point, for the identification.
  • the identification of the liquid sample can at the same time be an identification of the sample container into which the liquid sample is transported.
  • the control electronics can be set up to store the identifier and the time stamp locally in a memory of the control electronics and / or to a device connected for communication with the control electronics, e.g. a portable operating device (e.g. smartphone, tablet), one with the control electronics for communication connected PC or server to communicate.
  • the server can be a component of the sampling device, in particular a component of the control electronics or a computer remote from the sampling device, in particular a component of a cloud.
  • the automatic sampling device can furthermore have at least one interface for connecting the control electronics to at least one sensor which can be brought into contact with the sample liquid present at the sampling point and which is set up to receive measurement signals of at least one measured variable of the sample liquid, for example temperature, pH value or conductivity, to be generated and output to the control electronics, wherein the control electronics are further set up to store at least one measured value of the measured variable derived from the measurement signals, which is recorded at the point in time at which the liquid sample is taken from the extraction point, for the identification of the liquid sample. Using the additionally recorded measured variable, a measured value of the analyte concentration in the sample liquid determined on the basis of the liquid sample can be calculated more precisely.
  • the control electronics can be set up to store the measured values locally and / or to communicate with the above-mentioned device connected to the control electronics for communication.
  • turbidity concentrations of dissolved gases, ion concentrations or concentrations of photometrically or spectrometrically detectable molecules or sum parameters
  • SAK value spectral absorption coefficient
  • COD chemical oxygen demand
  • TOC total organically bound carbon
  • the automatic sampling device can optionally comprise means for adjusting a pH value in the liquid sample.
  • These means can include, for example, a pump controllable by means of the control electronics, possibly additional liquid lines and one or more reagents, e.g. one or more buffer solutions, one or more acids and / or one or more alkalis, which are transported into the sample container by means of the pump via the liquid lines can be used to adjust the pH of the liquid sample contained therein.
  • a pH sensor can be arranged in the sample container, which is connected to the control electronics in order to output measured pH values to it.
  • the control electronics can use these pH measured values to regulate the pH value in the liquid sample.
  • control electronics can be connected via the interface mentioned in the previous paragraph to a pH sensor immersed in the sample liquid at the sampling point, and one or more of the reagents based on the pH measurement value recorded by this pH sensor at the time the liquid sample was taken add to the liquid sample in the sample container to adjust the pH.
  • the sampling device can have a temperature sensor which is set up to detect measured values representing the temperature of a liquid sample contained in the at least one sample container and to output them to the control electronics.
  • the control electronics can also be set up to store at least one of the measured values or a measured value profile for the identification of the liquid sample.
  • the control electronics can be set up to store the measured values locally and / or to communicate with the above-mentioned device connected to the control electronics for communication.
  • a temperature or a temperature profile which the liquid sample with the analyte is suspended until the collection matrix or a sample provided by concentration and extraction of the analyte from the liquid sample is removed from the sample container or from the sampling device for the purpose of further analysis, are taken into account in the determination and / or interpretation of the analysis result.
  • the temperature profile can be used to determine the analyte concentration in the sample liquid at the time the sample is taken.
  • the temperature of the sample liquid can influence the rate of degradation processes of the analyte or analytes.
  • it can simultaneously influence the binding kinetics of the analyte (s) to the collecting matrix. It is therefore advantageous to record the temperature or the temperature profile to which the liquid sample and / or the collecting matrix is exposed after the liquid sample has been removed from the extraction point and to make it available for consideration in the qualitative and / or quantitative determination of the at least one analyte.
  • the automatic sampling device can comprise a temperature control device which is designed to control the temperature of the at least one sample container and the liquid sample contained therein, in particular to cool them.
  • the temperature control device can be designed to set a specific temperature in the area of the at least one sample container.
  • the sampling device can have a GPS receiver which is set up to output location data (GPS data) to the control electronics, the control electronics being set up to store the location data.
  • GPS data location data
  • the control electronics can be set up to store the location data locally and / or to communicate to the above-mentioned device connected to the control electronics for communication.
  • control electronics can have a communication interface.
  • the other device can, for example, be the already mentioned control device or a server that is set up to store the data, for example in a central and / or decentralized database, and software, for example a web or cloud application, for display or to make available for data analysis.
  • the software can be executed on the operating device, the server or on a further device, for example a further server or a PC, or in a cloud, of which the server can be a component. This enables the linking of liquid sample and / or sampling point-specific data, such as identifications, time stamps, temperature data, weather data, location data (e.g.
  • GPS data of the sampling point or measured values of the sample liquid, with those that can also be read into the control unit or the software, results of the qualitative and / or quantitative determination of the at least one analyte determined in the laboratory, hereinafter also referred to as analysis results for short, in the samples provided by the sampling point.
  • analysis results for short, in the samples provided by the sampling point.
  • the data, measured values and analysis results determined in this way can be used in a web or cloud application from Samples taken from different sampling points are brought together and compared with one another and / or with data from other sources.
  • the invention also comprises a measuring system for the qualitative or quantitative determination of at least one analyte, in particular a biopolymer, in a sample liquid, in particular water or wastewater, comprising:
  • An automatic sampling device for taking liquid samples of the sample liquid at a sampling point, e.g. a body of water or a container, in particular a pipe in a water supply or sewage network or an inlet or a basin of a sewage treatment plant, according to one of the configurations described above;
  • an analysis device set up for the qualitative or quantitative determination of the at least one analyte
  • a transfer device which is designed to provide a sample of the at least one analyte concentrated or extracted by means of the sampling device.
  • the transfer device can comprise a magnet which is set up to attract the magnetic beads after contact with the sample liquid and to transfer them into a further vessel. In this way, the collecting matrix with the analyte bound to it can be separated from the other components of the liquid sample. If, as mentioned above, the collecting matrix is contained in an insert of the sample container or in a removable cartridge, the transfer device can be designed to automatically remove the cartridge or the insert.
  • the collecting matrix with the bound analyte can be provided, for example, as a sample of the analyte concentrated or extracted by means of the sampling device for further analysis by means of the analysis device.
  • the transfer device can furthermore comprise means for eluting the analyte from the collecting matrix, here for example from the magnetic beads.
  • the eluate can be provided as a sample of the analyte concentrated or extracted by means of the sampling device.
  • the transfer device is further designed to transfer the prepared sample to the analysis device.
  • the measuring system can further comprise control electronics which are set up to control the transfer device for the automated transfer of a sample of the concentrated or extracted analyte to the analysis device.
  • control electronics can control pumps, valves and moving parts, such as the magnet mentioned above, to move the collecting matrix from an original position in the sample vessel or in a permeable unit in the fluid flow path of the sampling device into the analysis device or into a Transfer device belonging to another container to transfer and the analytes from the
  • control electronics can be the above-mentioned control electronics of the automatic sampling device or a component of the control electronics of the automatic sampling device.
  • the analysis device can be set up to carry out the qualitative or quantitative determination of the analyte in a partially or fully automated manner.
  • the analysis by means of the analysis device can be carried out, for example, using an affinity-based, in particular antibody-based, immunological assay or using a real-time qPCR-based method.
  • the analysis device can be set up to carry out the qualitative or quantitative determination of the analyte by means of a spectroscopic method, for example by means of Raman spectroscopy, in particular also by means of Surface Enhanced Raman Spectroscopy (SERS) or Tip Enhanced Raman Spectroscopy (TERS) by means of Mass spectrometry, or using hyperspectral imaging.
  • SERS Surface Enhanced Raman Spectroscopy
  • TMS Tip Enhanced Raman Spectroscopy
  • the analysis device can have a computing unit which is set up to determine a qualitative or quantitative analysis result, e.g. an analyte concentration in the original liquid sample, and which is further set up to control the analysis device for the automated determination of the analyte.
  • the computing unit can be set up to store analysis results of the qualitative and / or quantitative determinations in the samples provided by the transfer device, in particular each linked to an identification of the liquid sample from which a sample provided by the transfer device was obtained.
  • the computing unit of the analysis device can also have a communication interface for communicating data to another device.
  • the other device can be, for example, the control electronics of the sampling device, the control device already mentioned above or the server mentioned above, which is set up to store data provided by the analysis device, for example in a central and / or decentralized database, and a To provide software, e.g. a web or cloud application, for display or data analysis.
  • the software can be executed on the control device, the server or on another device, e.g. another server or a PC, or in a cloud, of which the server can be a component.
  • the software can link the analysis results made available by the analysis device with data of the associated liquid samples that were recorded by the sampling device and carry out a more extensive data analysis with the linked data.
  • the invention also comprises a method for the automated provision of a sample for a qualitative and / or quantitative determination of at least one analyte, in particular a biopolymer, in a sample liquid, in particular water or waste water.
  • the process consists of the following steps: - Removal of a predeterminable volume of the sample liquid as a liquid sample from the extraction point by means of an automatic sampling device, in particular the sampling device according to one of the configurations described above, from the at a sampling point, for example in a container or a body of water, in particular in a pipe in a water supply or Wastewater network, at an inlet point to a wastewater network or in an inlet or a basin of a sewage treatment plant, sample liquid present;
  • the method can also include extracting or concentrating the analyte by means of centrifugation or by means of a precipitation reaction.
  • the collecting matrix can be placed in the sample container or in a raw flow unit fluidly connected to the sample container, in particular a fluid line or a raw flow filter cartridge.
  • the collecting matrix can be configured as explained above in connection with the sampling device.
  • the method can further comprise removing the collecting matrix with the analyte from the device and / or eluting the analyte from the collecting matrix to form a solution of the analyte as a sample for the subsequent qualitative or quantitative determination.
  • the subsequent qualitative or quantitative determination of the analyte can be carried out by means of an affinity-based, in particular antibody-based, immunological assay, by means of a real-time qPCR-based method or by means of a spectroscopic method.
  • the result of this determination is referred to above and below as the analysis result for short.
  • the analysis result can represent the concentration of the analyte in the sample provided and / or the concentration of the analyte in the original liquid sample.
  • the method can further include storing an identification of the liquid sample and a time stamp representing the point in time at which the liquid sample was removed from the removal point in an electronic control system of the automatic sampling device.
  • the identification of the liquid sample and the associated time stamp can be stored by the control electronics and / or by a device that can be connected to the control electronics for communication, e.g. a, in particular portable, operating device or a server, and a software, e.g. a web or cloud application, which links the stored data with other data and, if necessary, carries out further data analyzes.
  • the method can record at least one measured value of at least one measured variable of the sample liquid, for example the temperature, the pH value or the conductivity, at the time the liquid sample is taken from the extraction point by means of an additional sensor and store the measured value in the control electronics to identify the Include liquid sample.
  • Further measured variables that can be measured by means of one or more additional sensors that can be connected to the control electronics are turbidity, concentrations of dissolved gases, ion concentrations or concentrations of photometrically or spectrometrically detectable molecules or sum parameters such as SAK value (spectral absorption coefficient), COD (chemical oxygen demand) or TOC (total organically bound carbon).
  • the at least one additionally determined measured value in particular several additionally determined measured values of one or more different of these parameters, can be stored linked to the identification of the liquid sample. They can be taken into account when determining or evaluating the analysis result determined on the basis of the sample provided.
  • the at least one additional measured value can be included in a calculation model for determining the concentration of the analyte in the original liquid sample from a qPCR measurement result or from a measurement value of an immunological assay or from spectrometric measurement data.
  • a pH value measured in the sample liquid can also be used to automatically set a pH value in the liquid sample that is advantageous for binding to the collecting matrix by means of the control electronics.
  • the sampling device can have storage containers with reagents that influence the pH value of the liquid sample, liquid lines, one or more pumps and, if necessary, one or more valves that are actuated by the control electronics to adjust the pH value of the liquid sample.
  • the method can further comprise the detection of a temperature or a temperature profile to which the liquid sample is exposed from the time it is removed.
  • the temperature or the temperature profile can be stored by the control electronics and linked to the stored identification of the liquid sample.
  • the temperature profile to which the liquid sample was exposed can be included in an evaluation and / or determination of the analysis result.
  • the stored temperature or the stored temperature profile can be included in a calculation model for determining the concentration of the analyte in the original liquid sample from a qPCR measurement result, from a measurement value from an immunological assay or from a spectroscopic measurement.
  • the method can further include the acquisition of location data that represent the location of the sampling point, for example by manual input or by a GPS receiver, and the storage of the location data in the control electronics.
  • the method can also include the transfer of the identification of the liquid sample and other data stored in the control electronics linked to the identification, e.g.
  • a time stamp associated with the identification, measurement values associated with the identification, location information associated with the identification and / or data specific to the sampling point to an operating device or an analysis device serving to analyze the provided sample, in particular to a laboratory device, or to a server connected to the analysis device and / or the control electronics for wireless or wired communication, which the data received from the control electronics and / or the analysis device and its memory can be accessed by software, for example a web or cloud application, in order to display the data and / or to carry out a further analysis of the data.
  • the software can be executed on the operator panel, the server or another device.
  • the invention also comprises a method for the qualitative or quantitative determination of at least one analyte, in particular a biopolymer in a sample liquid, in particular water or wastewater, comprising:
  • the method can be carried out with the measuring system described above.
  • the analysis method can be carried out fully or partially automatically on the sample provided by means of at least one analysis device which comprises a computing unit which is set up to determine a qualitative or quantitative analysis result, e.g. an analyte concentration in the original liquid sample.
  • a qualitative or quantitative analysis result e.g. an analyte concentration in the original liquid sample.
  • the analysis device can be an online analysis device which is arranged directly at the sampling point.
  • the sample provided can be brought to a laboratory and analyzed there in a partially or fully automated manner using at least one laboratory analyzer.
  • the sample provided by the method described above which is obtained by concentrating the analyte by means of a collecting matrix or alternatively also by another method, e.g. centrifugation, is less expensive to transport than a sample container containing the complete liquid sample. Because of the correspondingly smaller volume, cooling is also easier to achieve during transport.
  • the method can determine a concentration of the analyte in the original liquid sample with the additional inclusion of at least one measured value recorded at the sampling point, for example the temperature, the pH value or the Conductivity, or one or more further measured variables, the measured value being made available by the control electronics of the sampling device.
  • the concentration of the analyte in the original liquid sample also corresponds to the concentration of the analyte in the sample liquid that was present at the sampling point at the time the sample was taken.
  • the time of sampling can be represented by a time stamp, which is recorded by the control electronics of the sampling device and stored in the control electronics linked to the identification of the liquid sample and / or from the control electronics to another device, e.g. the analysis device, to an operating device or a Server, can be issued for storage associated with the identification of the liquid sample.
  • the sampling point can be represented by location information, for example GPS data, which is recorded by the control electronics and stored by them and / or linked for storage with the identification of the liquid sample to another device, e.g. the analyzer, to the control unit and / or a server, which can be issued with the identification of the liquid sample for storage.
  • the control electronics of the sampling device can transmit the measured values to be included in the determination of the concentration of the analyte in the liquid sample together with the identification of the liquid sample to the analysis device for storage.
  • the values, together with the identification of the liquid sample can be read from the control electronics into an operating device for storage and output from the operating device to the analysis device and stored there.
  • the values can be output together with the identification of the liquid sample from the control electronics to a server, which stores the values and makes them available to software, e.g. a web or cloud application.
  • the analysis device or the computing unit of the analysis device can output the analysis results of the sample, e.g.
  • the control electronics, the control unit, the server or the other server stores the analysis results linked to the identification of the liquid sample.
  • the software can access the control unit, the server or the other server in order to read out the stored analysis results and the associated identification and use them for further data analysis.
  • the quantitative determination of the analyte e.g. the determination of an analyte concentration in the original liquid sample including the at least one further measured value, can be carried out in one embodiment of the method by the computing unit of the analysis device using the data provided by the operating device or the software.
  • the quantitative determination of the analyte can be performed by the software using an analysis result determined by the computing unit of the analysis device from the sample and output to the server and, if necessary, by the control electronics of the Sampling device detected and output to the server measured values are carried out.
  • the analyte concentration in the original liquid sample can be calculated using one or more measured values using a calculation model.
  • the calculation model can, for example, take into account the influence of the temperature on the binding behavior of the analyte to a collecting matrix for the concentration and / or extraction of the analyte in the liquid sample and the influence of the temperature on the degradation process.
  • measured temperature values or measured values of the temperature profile to which the liquid sample was exposed before the analysis can be entered by the sampling device.
  • the software can be run on the operator panel, the server, another server or distributed in a cloud.
  • the additional measured values recorded by the control electronics and associated with the liquid sample can not only be used to determine a concentration of the analyte. They can be used as an alternative or in addition to evaluating the analysis result, or to create or adapt chemometric or epidemiological models. They can also be used for statistical purposes.
  • the method can further comprise the communication of the analysis result and possibly additional data from the control electronics and / or the analysis device to the software mentioned, e.g. a web or cloud application, for further analysis.
  • the additional data can be an identification of the sampling point, e.g. GPS data or other location data that identify the sampling point.
  • the software in particular in the form of a cloud application, can access data from a large number of sampling devices at a large number of sampling points.
  • the data in particular identifications of liquid samples, linked with associated time stamps, location information and measured values, can be stored in a central database or in a cloud on several computers, which the software can access.
  • the analysis results that are obtained by means of the qualitative or quantitative determinations of the analyte carried out by the liquid samples collected by the sampling devices can also be stored in the database in association with the identifications of the associated liquid samples.
  • the software can use the data for comprehensive data analysis.
  • the software can, for example, monitor a temporal course of the analysis results and thus provide an early warning system that detects an increase in the concentration of the analyte at a sampling point or in an area comprising several sampling points and generates a warning based on this.
  • the software can also use the data to analyze a temporal and / or spatial development of the analyte concentration in an extensive area, for example in a water or sewage network of a larger region or an extensive body of water. It can also provide a visualization in the form of a map of the analyte concentration. Trends, ie developments over time, can also be displayed on the map.
  • the software can make the results of the data analysis, in particular the mentioned displays of information from an early warning system or graphic representations of maps, available to other computers (end devices), in particular in a browser-based application on the end devices.
  • the above devices and methods are suitable, for example, for monitoring water or sewage networks, areas in sewage treatment plants and discharge points in sewage networks for the occurrence or trends in the concentration of pathogens, e.g. viruses or bacteria, or parts of pathogens, e.g. DNA, RNA or Shell components, in the water.
  • pathogens e.g. viruses or bacteria
  • pathogens e.g. DNA, RNA or Shell components
  • This can be used for modeling, forecasting and analyzing epidemics, especially as an early warning system.
  • pathogen can be, for example, the Sars-CoV-2 virus.
  • FIG. 2a shows a detail of the automatic sampling device in a first embodiment
  • FIG. 3 shows a measuring system for the qualitative or quantitative determination of an analyte in a
  • FIG. 4 shows a measuring system for the qualitative or quantitative determination of an analyte in a
  • Wastewater sample in a second embodiment.
  • an automatic sampling device 1 is shown. It is designed as a cabinet unit that is divided into an upper dosing space and a lower sample space.
  • the dosing space can be closed with a dosing space door 2, the sample space with a sample space door 3.
  • a pump 4 which in the present example is designed as a hose pump, is arranged in the metering chamber. Other configurations, for example as a vacuum pump, are possible.
  • the pump 4 interacts with a sample line 5, which in the present example is designed as a hose line.
  • the sample line 5 can be fluidically connected at its first end (not visible in FIG. 1) to a sampling point 12 (FIGS. 2a, 2b) from which liquid samples of a sample liquid can be taken for analysis.
  • the sampling point can be an open body of water or a channel.
  • the fluidic connection can be established by immersing the first end of the sample line 5.
  • the sampling point 12 can also be a closed container, for example a pipe, through which the sample liquid flows. In this case, the fluidic connection can be established by means of a sampling valve.
  • the second end of the sample line 5 is fluidically connected to a sample distributor 6 designed here as a rotary arm.
  • a sample distributor 6 designed here as a rotary arm.
  • the sample distributor can be moved over the sample container 8 by means of a distribution mechanism, here a rotating mechanism, in order to fluidically connect the sample line 5 to one of the sample containers 8.
  • a distribution mechanism here a rotating mechanism
  • a fluid flow path is formed which runs from the sampling point 12 via the sample line 5 and the sample distributor 6 into the sample container 8.
  • a distributor plate 9 is arranged above the sample container 8 in the present example. However, this is only available as an option.
  • a filter or a filter cascade can be arranged at the first end of the sample line 5, which filter back at least some of the solids contained in the sample liquid by size filtration.
  • the filter or filters can also be arranged at another point in the fluid flow path between the first end of the sample line 5 and the sample container 8.
  • the sampling device 1 further comprises control electronics 10 which are set up to control the pump 4 and the sample distributor 6 for transporting a predeterminable volume of the sample liquid from the extraction point into a sample container 8.
  • control electronics 10 which are set up to control the pump 4 and the sample distributor 6 for transporting a predeterminable volume of the sample liquid from the extraction point into a sample container 8.
  • a window 11 through which a display of the control electronics 10 can be seen and the control electronics 10 can be operated by means of input keys and a turn-push switch even when the dosing room door is closed.
  • the control electronics 10 have a processor and memory in which operating and evaluation programs are stored that the control electronics 10 can carry out for controlling the sampling device 1 for sampling and for recording and storing data in connection with the sampling.
  • the sampling device 1 can optionally have a cooling device which serves to cool the sample space. A temperature of the sample space can be set by means of the control electronics 10.
  • the control electronics 10 can in particular be set up to store an identification for a sample transported into a sample container 8, as well as a time stamp assigned to the identification, which identifies the point in time at which the sample was taken.
  • the control electronics 10 can Assign and store further information on the identification, e.g. measurement data of other sensors connected to the control electronics 10, such as temperature measurement data, pH measurement data, conductivity measurement data, turbidity measurement data, measurement values of these parameters and location data, e.g. the location of the sampling point at which the sampling device 1 is arranged.
  • the control electronics 10 also have one or more communication interfaces via which they can communicate with further data processing devices, for example with an analysis device that is used to analyze the samples provided by the sampling device 1, or with a portable control unit via which an operator can collect data from the Can read control electronics 10 or can output to the control electronics 10.
  • the control electronics 10 can also be set up to transmit data to a server, which stores the data and makes it available to software, for example a web or cloud application. These data can include the identifications and associated data of collected liquid samples, as well as location data identifying the sampling point.
  • the software can access this data for further evaluation and analysis. Conversely, the software can make data or information available to the control electronics 10 via the server for future sampling or for display on a user interface of the control electronics 10.
  • the control electronics 10 can have one or more of the following types of communication interfaces: communication interfaces according to a standard of the process industry, e.g. 4 ... 20 mA, Profibus, HART, Modbus, or other, in particular also proprietary communication standards; but also communication interfaces for communication according to an Ethernet standard or a Bluetooth standard.
  • the sampling device 1 also has means for concentrating and / or extracting an analyte comprising a biopolymer taken from one of the sampling point.
  • the analyte can be, for example, a virus or a virus component or a bacterium or a bacterial component.
  • Components of viruses and bacteria are understood here to mean in particular parts of their shells, in particular proteins, and polynucleotides such as RNA or RNA fragments, DNA or DNA fragments. Examples of means for concentrating and / or extracting such an analyte are described below with reference to FIGS. 2a and 2b.
  • FIG. 2a and 2b schematically show the fluid flow path between the sampling point 12 (here an indicated channel) and a sample container 8 (the sample distributor is omitted in this illustration for the sake of clarity).
  • a flow meter 13 which is used to set the predeterminable sample volume.
  • the predeterminable volume of the sample liquid can be transported into the sample container 8 via the sample line 5 by means of the pump 4.
  • a collecting matrix in the form of a plurality of magnetic or polymer beads 14 is provided in the sample container 8, the surfaces of which are modified in such a way that the analyte in the sample liquid essentially binds selectively to the surfaces.
  • the analyte is, for example, virus RNA of a certain type of virus
  • RNA of such viruses binds to the beads, but not - or at least to a much lesser extent - other RNA or DNA in the sample liquid.
  • Such beads are known in the prior art for the enrichment and / or extraction of biomolecules, in particular biopolymers. Their surfaces can be modified by structuring or functionalization with antibodies or other capture structures in order to provide the desired selectivity.
  • the sampling device can comprise one or more further liquid containers 15 with one or more reagents, e.g. an acid, a base and / or a buffer solution, as well as a further pump 16, by means of which the reagent or reagents are added to the liquid sample can.
  • the further pump 16 is a hose pump which interacts with a liquid line 17 in order to transport a reagent from the liquid container 15 into the sample container 8.
  • the pump 16 can be controlled by the control electronics 10 of the sampling device 1 in order to dose an amount of the reagent or reagents required to achieve a desired pH value.
  • a pH sensor can be provided in the sample container, which outputs pH measured values to the control electronics 10 so that the latter can control the addition of the reagent or reagents on the basis of the pH measured values.
  • the control electronics 10 can also be connected to a pH sensor immersed in the sample liquid present there at the sampling point 12 and use pH measurements from this sensor to control the addition of the reagent or reagents to the liquid sample in the sample container 8.
  • the beads 14 with the analyte bound or immobilized thereon can be removed from the sample container 8 manually or by means of a (partially) automated transfer device (not shown in FIG. 2a) and transported to a laboratory for further analysis.
  • a magnet in particular a switchable electromagnet, can be used which, for example, can be moved in an automated manner to remove the magnetic beads and can be controlled by the control electronics 10.
  • another possibility for removing the beads 14 from the sample container 8 can be to automatically empty the sample container 8 while retaining the beads 14 in a filter.
  • the beads 14 can also be held in an insert in the sample container 8 and can be removed from the sample container 8 by removing the insert. If there is a transfer device, it can also comprise means for eluting the analyte from the beads, so that the sample provided for transport to the laboratory or for transfer to an analysis device for further qualitative or quantitative analysis is formed from the eluate.
  • FIG. 2b Another embodiment of the means for concentrating and / or extracting the analyte is shown in FIG. 2b.
  • these means comprise a removable cartridge 17 which is arranged in the upper region of the sample container 8 and which contains a collecting matrix with an affinity for analytes.
  • This can be, for example, a functionalized fleece, a chromatography column or a bed of surface-functionalized polymer beads.
  • the beads or the fleece can be functionalized in an analogous manner to the magnetic or polymer beads used in the example in FIG. 2a.
  • the analyte in the cartridge is essentially selectively bound to the collecting matrix or, in the case of a chromatography column, separated chromatographically from other sample components and retained in the collecting matrix.
  • the cartridge 17 can also be arranged outside the sample container 8 at a different position in the fluid flow path.
  • the cartridge 17 can be removed automatically or manually from the sample container or from the fluid flow path in the sampling device 1 and transported to a laboratory for further analysis. If the cartridge 17 is removed automatically by means of a transfer device, this can, as in the example described above, additionally comprise means for eluting the analyte from the beads, so that they can be transported to the laboratory or transferred to an analysis device for further qualitative or quantitative analysis prepared sample is formed from the eluate.
  • means for concentrating and / or extracting the analyte are conceivable, e.g. a centrifugation device which can be integrated in the sampling device 1.
  • a measuring system 100 for the qualitative or quantitative determination of the analyte contained in the sample liquid by means of liquid samples taken from the sampling point 12 is shown schematically in FIG. 3.
  • the measuring system 100 comprises the sampling device 1 with the control electronics 10 already described with reference to FIGS. 1, 2a, 2b a sewage treatment plant.
  • the additional sensor 18 can be a conductivity sensor, a pH sensor, a turbidity sensor, a temperature sensor, a photometric or spectrometric probe for determining a concentration of one or more Substances, in particular a nitrate or SAC probe, or an ion-selective electrode.
  • the additional sensor 18 outputs measured values to the control electronics 10 of the sampling device 1. As already mentioned in connection with FIG.
  • control electronics 10 are set up to link an identification of a removed liquid sample, as well as a time stamp identifying the time of sampling and other data, in particular measurement data of the additional sensor 18, with the identification and to store it .
  • the sampling device 1 can also have several additional sensors 18 connected to the control electronics 10, the measured values of which can be recorded and stored.
  • the measuring system 100 also has an analysis device 20, which is arranged in a laboratory remote from the sampling point 12, which has a liquid handling system for sample preparation and preparation as well as an analysis unit for the subsequent automated implementation of an immunological assay or a real-time qPCR-based or a spectroscopic analysis of the sample, as well as a computing unit includes.
  • the sample obtained from a liquid sample taken from the sampling device 1 by concentrating and / or extracting the analyte for further analysis is transferred to the analysis device 20 and analyzed from the sample in an automated or partially automated manner using this method known per se in the prior art.
  • the computing unit is used, among other things, to calculate a quantitative analysis result, e.g. a concentration of the analyte in the sample provided by the sampling device 1 or in the original liquid sample, and possibly also to control liquid handling and analysis procedures carried out automatically by means of the analyzer 20 .
  • the analysis device 20 can be connected to the sampling device 1 for communication, for example via a LAN connection (intranet or Internet).
  • the sampling device 1 can use this connection to transmit the identifications of liquid samples and associated information such as sensor data, time stamps, and location information to the computing unit of the analysis device 20.
  • the computing unit of the analysis device 20 can transmit analysis results together with the identification of the associated liquid samples to the sampling device 1.
  • the computing unit of the analysis device 20 can use the additional information received from the sampling device 1 to determine and / or evaluate the analysis results of the individual samples. For example, when determining a quantitative analysis result, the influence of other measured variables of the sample liquid, e.g. the pH value or the conductivity of the sample liquid at the time of sampling, can be taken into account.
  • the merging of the analysis results determined by the analysis device 20 and the additional information made available by the sampling device 1 takes place on the basis of the identifications of the samples.
  • the measuring system 100 can optionally include a mobile operating device 21, which in the present example is designed as a smartphone or tablet PC.
  • Operating device 21 can be set up for bidirectional communication both with the sampling device 1 and with the analysis device 20. It can therefore be used to transfer data from one unit to the other. It can also be used to read in a label attached visibly to a sample container 8 or readable in an NFC or RFID chip on the sample container 8 in order to avoid confusion when transferring the concentrated samples from the sampling device 1 to the analysis device 20 in the laboratory.
  • the methods and means for unambiguously identifying sample containers used to identify and manage collected samples in sample containers during transport between a sampling point and a laboratory are well known to the person skilled in the art and can also be used here. It is therefore not discussed in detail here.
  • the operating device 21, the analysis device 20 and the control electronics 10 of the sampling device 1 are configured in the present example for communication with a server which, in the present example, is part of a cloud 22.
  • the server can also be a local computer, i.e. located at the sampling point or in the laboratory, or an individual central computer that can communicate with the computing unit of the analysis device 20, the operating device 21 and / or the control electronics 10 via the intranet or Internet.
  • the server can collect and save data, measured values and analysis results linked to the identification of the associated sample and use them for data analysis.
  • the storage can take place in a central database or in the cloud.
  • a computer that can be connected to the server, e.g. another server, a PC or the operating device, or in the cloud 22, software can be executed that accesses the stored data and carries out further data analyzes and / or data and results of the Can bring data analyzes to the display.
  • This software can be a web or cloud application, for example.
  • the software or the cloud 22 can be connected to a large number of sampling points and laboratory analysis devices and thus determine and analyze a large number of data from spatially distributed sampling points.
  • a cloud application can, for example, record and evaluate quantitative or qualitative analysis results for certain analytes, such as viruses or other microorganisms, in particular pathogens, in a body of water, a water or sewage network or in a large number of different private and / or municipal sewage treatment plants. It can monitor changes in the concentration of the analyte over time, e.g. a virus concentration at the individual sampling points or in a larger region comprising several sampling points, and thus serve as an early warning system for the outbreak of a disease or epidemic in the monitored region.
  • the cloud application can provide a map of the concentrations of the analytes at the various sampling points.
  • the cloud application 22 can be set up to compare the analysis results determined for the individual sampling points and other data with data from other sources. If the analyte is, for example, a virus or a component of the virus, e.g.
  • the concentration of the virus or the virus - RNA in regional wastewater can be compared with data from regional health authorities in order to predict outbreaks of a disease caused by the virus at an early stage, to increase the number of locally performed tests on patients or to identify discrepancies between tests on patients and analysis results in wastewater.
  • FIG 4 shows a further exemplary embodiment for a measuring system 101 which is used for the qualitative or quantitative determination of the analyte contained in the sample liquid by means of liquid samples taken from the sampling point 12.
  • additional data e.g. time and location data as well as additional measured values, is set up.
  • the measuring system 101 further comprises an analysis device 20 which is used for the qualitative or quantitative determination of the analyte in samples provided by the sampling device 1.
  • the measuring system 101 described here has a transfer device 23 that can be operated completely automatically and is designed to automatically assign a collecting matrix from the sample container 8, which is contained in a sample container 8 of the sampling device 1 and essentially selectively binds the analytes remove.
  • the collecting matrix is formed from a multiplicity of surface-modified magnetic beads which are placed in the sample container 8 in such a way that analyte present in the liquid sample forms on the magnetic beads.
  • means for adjusting the pH value in the liquid sample can also be provided.
  • the transfer device 23 has one or more movable and / or switchable magnets 24 for removing the collecting matrix from the sample container 8, with which the magnetic beads can be attracted and removed from the sample container 8.
  • the magnetic beads are rinsed with solvent in the transfer device 23 and the analyte is eluted from the collecting matrix.
  • the liquid sample obtained in this way is transferred from the transfer device 23 via a fluid line 25 into the analysis device 20.
  • the transfer unit can be controlled by means of a local control unit or by means of the control electronics 10 of the sampling device 1.
  • the analysis device 20 can be designed analogously to the analysis device described above with reference to FIG. 3 for the automated implementation of a further sample preparation and for the subsequent implementation of an analysis, for example an immunological assay, a real-time qPCR-based analysis or a spectroscopic analysis of the sample.
  • the analysis device 20 comprises a computing unit 26 and an analysis cartridge 27 in which the liquid analysis is carried out in a controlled manner by means of the computing unit 26.
  • the computing unit 26 is connected to the control unit 10 for communication via cable 28 or wirelessly in order to read in identifications and associated data of the samples to be analyzed and / or to return analysis results to the control electronics 10.
  • the control electronics 10 and / or the computing unit 26 can communicate with a server, which in the present example is part of a cloud 22.
  • a server which in the present example is part of a cloud 22.
  • Data exchange and analysis as well as further evaluations of the data and analysis results made available by means of a cloud application accessing the data stored in the server can take place in a completely analogous manner to that described above with reference to FIG. 3
  • the sampling device 1 described here and the measuring systems 100 and 101 can be used for process-compatible, systematic removal of liquid samples, in particular from water supply networks, sewage networks and sewage treatment plants, with subsequent analysis for the qualitative or quantitative determination of analytes comprising biopolymers, such as viruses, bacteria or DNA or Serve RNA.
  • the data can be made available systematically, in regular measurement cycles and with little personnel and time expenditure, and advantageously from a web or cloud application that can be accessed by a large number of end devices and that links the sample data with other data from other sources can be further analyzed or made available for further analyzes by authorities or scientific research institutions.
  • this enables comprehensive studies on the course of epidemics; on the other hand, a reliable early warning system can be made available in the event of an increased occurrence of pathogens in the supply or wastewater.

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Abstract

L'invention se rapporte à un appareil d'échantillonnage automatique (1) permettant de prélever des échantillons liquides d'un échantillon liquide, en particulier d'eau ou d'eaux usées, présentes au niveau d'une station d'échantillonnage (12) d'un corps d'eau ou d'un récipient, pour la détermination qualitative ou quantitative d'au moins un analyte, en particulier d'un biopolymère, contenu dans le liquide échantillon, l'appareil comprenant : • une conduite d'échantillon (5) pouvant être en liaison fluidique avec la station d'échantillonnage (12) ; • un dispositif de pompe (4) ; • au moins un récipient d'échantillon (8) pouvant être en liaison fluidique avec la conduite d'échantillon (5) ; et • un système de commande électronique (10) configuré pour la liaison fluidique de la conduite d'échantillon (5) avec l'au moins un récipient d'échantillon (8) de telle sorte qu'un trajet d'écoulement de fluide s'étendant depuis la station d'échantillonnage (12) à travers la conduite d'échantillon (5) dans le récipient d'échantillon (8) soit formé, • le système de commande électronique (10) étant en outre configuré pour transporter, au moyen du dispositif de pompe (4), un volume définissable du liquide d'échantillon, sous la forme d'un échantillon liquide, le long du trajet d'écoulement de fluide dans le récipient d'échantillon (8), • l'appareil d'échantillonnage (1) comprenant des moyens (14, 17) de concentration et/ou d'extraction de l'analyte présent dans l'échantillon liquide.
PCT/EP2021/060101 2020-05-15 2021-04-19 Appareil et procédé d'échantillonnage automatique permettant la fourniture automatique d'un échantillon pour la détermination qualitative et/ou quantitative d'un anaylte WO2021228499A1 (fr)

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US17/998,847 US20230194390A1 (en) 2021-04-19 2021-04-19 Automatic sampling apparatus and method for automatically providing a sample for qualitative and/or quantitative determination of an analyte
CN202180033791.6A CN115516287A (zh) 2020-05-15 2021-04-19 自动取样设备和用于自动提供样本以定性和/或定量确定分析物的方法
EP21720435.3A EP4150311A1 (fr) 2020-05-15 2021-04-19 Appareil et procédé d'échantillonnage automatique permettant la fourniture automatique d'un échantillon pour la détermination qualitative et/ou quantitative d'un anaylte

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DE102020113317.0A DE102020113317A1 (de) 2020-05-15 2020-05-15 Automatische Probennahmevorrichtung und Verfahren zum automatisierten Bereitstellen einer Probe für eine qualitative und/oder quantitative Bestimmung eines Analyten
DE102020113317.0 2020-05-15

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