WO2021211080A1 - Micropipette multicanal fonctionnelle (plage de volume élevée) - Google Patents

Micropipette multicanal fonctionnelle (plage de volume élevée) Download PDF

Info

Publication number
WO2021211080A1
WO2021211080A1 PCT/TR2021/050327 TR2021050327W WO2021211080A1 WO 2021211080 A1 WO2021211080 A1 WO 2021211080A1 TR 2021050327 W TR2021050327 W TR 2021050327W WO 2021211080 A1 WO2021211080 A1 WO 2021211080A1
Authority
WO
WIPO (PCT)
Prior art keywords
pipette
chamber
multipipette
micropipette
volume
Prior art date
Application number
PCT/TR2021/050327
Other languages
English (en)
Inventor
Mehmet Ali ÖZARGUN
Toprak İzgi GÜVEN
Sümer Erkan KAYA
Original Assignee
Oezargun Mehmet Ali
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oezargun Mehmet Ali filed Critical Oezargun Mehmet Ali
Publication of WO2021211080A1 publication Critical patent/WO2021211080A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means

Definitions

  • the invention belongs to a micropipette device commonly used in medical, chemistry, biology and biochemistry laboratories for precise liquid measurement. Certain experiments, ranging from university laboratories to clinical research laboratories, require different micropipettes. For example, ⁇ 250m1> chloroform and ⁇ 15-25m1> TE buffer should be used in RNA isolation experiments. If reference is made to commercially available micropipettes, the use of two different pipettes is mandatory. The invention combines these two pipettes while offering a wider volume range.
  • micropipette The multichannel micropipette is produced from the idea of a micropipette device, which is a sensitive measuring device in origin and used for liquid measurement.
  • Micropipettes are widely used in biology, chemistry indexed laboratories. It is used in many experiments to make precise and spot measurements. Over the years, micropipettes have been developed to provide ease of experimentation and to extend their functions.
  • a multichannel micropipette is an advanced micropipette that offers the same volume draw to multiple outlets with the same plunger. This model is frequently used for experiments requiring more samples of the same volume. The operating principle is the same as for single chamber micropipettes.
  • a multichannel pipette which is recognised with its optionally tuned and managed to increase the availability of the multichannel pipette and improve productivity, quality control and sustainability in a research area.
  • the pipette comprises of a pipette main body, a lower section connected to the lower end of the pipettes main body, a plural chip holding units disposed within the lower part; and removable by any of a plurality of connectable sections located in a unit casing within the lower section where each of the chip holder units may be attached and removed from it.
  • the pipette known in US 2018318824 differs from the pipette of the present invention in the following aspects.
  • the multichannel micropipette draws the same volume from different outlets in its model, while the multichannel micropipette according to the invention (100) is capable of withdrawing the desired volume of solution independently from the compartment of that particular volume. Since the pipette of the present invention has a total chamber of ⁇ 3>, it is called multichannel.
  • Single channel electronic pipettes are known from CN 108031501.
  • Electronic pipettes are high- tech smart micropipettes.
  • An electronic pipette comprising an electronic pipette with a battery- powered system therein, a piston actuated in a cylinder located within an engine, a control system for performing pipette operations and a user interface for operating the pipette; which includes at least one touch or press area of the touch screen to perform pipetting operations.
  • the electronic pipette also relates to the use of a touch screen as part of the user interface of an electronic pipette to run pipetting operations.
  • the invention (100) provides more than the volume range offered by the electronic pipette in addition to its cheap cost and allows the use of a single pipette for each experiment. This reduces the time the researchers spent in the laboratory because of the limited number of pipettes, allowing researchers to complete the entire experiment with a single model.
  • a handheld electronic pipette is known to increase ease of use.
  • the invention relates to a hand-held electronic pipette, the contents of which comprise a linear actuator comprising a motor for driving a piston to aspirate and dispense a fluid into and out of a pipette tip; a control circuit for the pipette comprising a user-controllable microprocessor and memory; a screen electrically connected to the microprocessor; and a user operable controller connected to the microprocessor.
  • the controller can be operated by the user along at least one axis to navigate and select at least one option in the user interface.
  • the present invention adds diversity to the prior art, combining the different volume subsets of the model that are required for the experiments.
  • the model thus facilitates the purchase of laboratory equipment economically and ergonomically. In terms of use, the comfort of the existing model is preserved. It is compatible with polypropylene pipette tips, which are available in the market and are currently used for single use.
  • Model allows ⁇ 3> different interchangeable compartments.
  • Another feature of the manual micropipettes is that they can be easily disassembled and reassembled for maintenance. These pipettes should be cleaned and maintained at regular intervals, and if necessary, the volume calibration setting should be corrected.
  • a problem with the new technology electronic pipettes is that maintenance cannot be carried out easily.
  • the invention is designed to be easily disassembled and cleaned in each ⁇ 3> chamber.
  • the invention mentioned above is a product to be produced like any other micropipette, except for its external design.
  • ⁇ Figure 4> and ⁇ Figure 5> refer to inner parts of the invention from the cross-section view; ⁇ Figure 6> and ⁇ Figure 7> refer to detailed exploding view of the upper parts and lower parts, alongside with the main body.
  • the operating principle of the invention (100) is the same as the single channel micropipettes if the chamber is handled independently.
  • the plunger (5) is placed in the desired chamber and locked.
  • the model is used by holding the multichannel body (2) with the thumb on the key part (5) of the plunger.
  • the cap (4) of the plunger is provided for the comfort of the thumb and can be in different colours according to the user's request.
  • the finger support (1) has been added for user comfort.
  • the key (5) is placed by connecting to the magnet plunger (7; 8) to the desired chamber.
  • the desired volume adjustment is made on the volume indicator (3) by turning the key piece (5).
  • the plunger (5; 8) is connected to the main piston with the help of the transition cylinder (9).
  • the rotation process changes the numbers on the volume indicator wheels (17) where the main piston (14) is clamped, and the desired volume can be attained.
  • the calibration cycle (6) can be turned and the adjustment can be made again.
  • the plunger (5; 8) is connected to the main piston (14) through the transition cylinder.
  • the piston slide (15) which is located around the main piston (14) for the friction, protects the mechanism and provides linear movement during these operations.
  • the upper, middle and lower stabilizers (13; 16; 18), on the other hand, are maintaining these common parts and protect the integrity. As stated earlier, the peculiar mechanisms of each chamber start from their sub-stabilizers.
  • the lower inner piston (23) passes through the volume wheels (17) and contacts with the main piston (14).
  • the air flow created by the plunger (5; 8) is transmitted through the buffer spring and the main spring (19; 21).
  • the buffer spring and the buffer cylinder (24; 25) are included to maintain the integrity of the mechanisms. These mechanisms stay clamped inside the lower outer piston (32). They are located inside the outer shaft (38) to protect it from both physical damage and chemicals intended for use in experiments.
  • the outer shaft (38) is also located in the other two chambers for the same reason and includes the opening suitable for attaching the pipette tip.
  • these outer shafts (36;37;38) can be removed by rotating manually. It is recommended to wear laboratory gloves for this process.
  • the pipette tips used in the experiments are for single use only and must be changed with each new solution. These pipette tips work integrated with ejector systems since they pose a risk of contamination as a result of a direct user contact.
  • three ejector buttons can be found on the upper surface, connecting to their rods (10) inside the multichannel body(2A;2B;2C).
  • This main body is clamped with ejector connecting rods (33; 34; 35) and with its downward linear movement, it can push the pipette tip out without direct user contact.
  • ejector connecting rods (33; 34; 35) can be removed by the user in the pressed position of the ejector button (10) and can be re attached the same way.
  • each chamber (2A;2B;2C) has its own ejection button (10), eliminating the possibility of errors with its chamber-specific colour. This process also prevents the user from being exposed to contamination as well as damaging the sensitive measuring mechanism from the solution.
  • the plunger (5;8) is used for both draining and discharging liquid.
  • the fluid in that volume range (3) can be taken and released.
  • the basic procedure is to hold the plunger (5;8) to the first stop by holding the piston in the air.
  • Fluid intake is achieved by releasing the button slowly and evenly.
  • the micropipette (100) is taken with the liquid to the place where the liquid is to be discharged.
  • the plunger (5;8) is pressed again to the first stop, then continued to the last point (second stop). This will release all the liquid from the pipette tip.
  • volume adjustable pipettes the volume at the tip of the pipette can be brought to the desired microliter.
  • Most models have volume indicators (digital etc.) which shows the volume that pipette tip draws.
  • the most common models range from 0.5- 10 m ⁇ , 10-100m1, 100-1000m1.
  • the invention has these ranges in its 3 chambers.
  • the invention (100) provides the function of the 3 micropipettes.
  • 0.1-2m1, 0.1-10m1, 20- 200m1, 1000-5000m1 models can be produced and replaced with each chamber (2A;2B;2C) of the invention mentioned above.
  • the invention can be manufactured by lighter material.
  • the angle of the finger support (1) can be adjusted and produced accordingly for user comfort.
  • plastic pipette tips used are designed for aqueous (liquid) solutions and are not recommended for use with organic components (can melt pipette tips or damage the pipette mechanism)

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Cette micropipette, qui est produite afin d'utiliser les solutions requises dans de nombreuses expériences et de les prendre dans différents volumes dans un seul dispositif, comprend 3 compartiments sans contact avec différentes plages de volume pour différentes sorties de pipette et différentes solutions. Les solutions souhaitées sont retirées dans l'ordre souhaité et dans un volume approprié. L'utilisateur contrôle chaque compartiment avec un seul piston contenant un aimant et peut le décharger dans le tube à essai approprié à n'importe quel moment. De plus, le principe de fonctionnement de l'invention est le même que les micropipettes actuellement utilisées et le confort d'utilisation est maintenu. Les nouvelles parties de l'invention sont conçues pour être rentables.
PCT/TR2021/050327 2020-04-14 2021-04-08 Micropipette multicanal fonctionnelle (plage de volume élevée) WO2021211080A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2020/05922 2020-04-14
TR202005922 2020-04-14

Publications (1)

Publication Number Publication Date
WO2021211080A1 true WO2021211080A1 (fr) 2021-10-21

Family

ID=75581590

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2021/050327 WO2021211080A1 (fr) 2020-04-14 2021-04-08 Micropipette multicanal fonctionnelle (plage de volume élevée)

Country Status (1)

Country Link
WO (1) WO2021211080A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3641593A1 (de) * 1985-12-06 1987-06-11 Fuji Photo Film Co Ltd Doppelpipettenvorrichtung
JP2016182600A (ja) 2011-05-17 2016-10-20 ライニン インストルメント、エルエルシー 二軸コントローラを有する電子ピペット
CN108031501A (zh) 2012-02-13 2018-05-15 恩姆菲舍尔科技公司 电子吸液管
DE112016006174T5 (de) * 2016-01-07 2018-09-20 A & D Company, Limited Mehrkanalpipette

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3641593A1 (de) * 1985-12-06 1987-06-11 Fuji Photo Film Co Ltd Doppelpipettenvorrichtung
JP2016182600A (ja) 2011-05-17 2016-10-20 ライニン インストルメント、エルエルシー 二軸コントローラを有する電子ピペット
CN108031501A (zh) 2012-02-13 2018-05-15 恩姆菲舍尔科技公司 电子吸液管
DE112016006174T5 (de) * 2016-01-07 2018-09-20 A & D Company, Limited Mehrkanalpipette
US20180318824A1 (en) 2016-01-07 2018-11-08 A&D Company, Limited Multichannel pipette

Similar Documents

Publication Publication Date Title
EP3096149A1 (fr) Dispositif de pipettage en pénétration à bouchon souple, et procédé de pipettage en pénétration à bouchon souple
JP6600642B2 (ja) 互いに重ねられる２つの吸引室を備えるマルチチャンネルピペット操作システム
EP0265028B1 (fr) Pipette à canaux multiples
US6244119B1 (en) Multichannel pipette system and pipette tips therefor
KR101449086B1 (ko) 피스톤의 전후 운동에 의한 샘플링을 제공하는 피펫
WO2001066253A1 (fr) Pipette volumetrique manuelle ergonomique sans ressort de rappel
US11660592B2 (en) Dynamic broad volumetric range pipette
EP3677921A1 (fr) Une cartouche pour système de dosage numérique
US7204163B2 (en) Mechanical piston pipette
EP1206321B1 (fr) Bande souple pour pipette et son procede d'utilisation
WO2021211080A1 (fr) Micropipette multicanal fonctionnelle (plage de volume élevée)
JP6367216B2 (ja) ピペットチップ、そのようなチップを備えるピペット、そのようなピペットチップと、試料を含有する少なくとも１つの容器とを含む一式及びそのようなピペットを使用する方法
US7462328B2 (en) Pipette for disposable tips of different size
US6074611A (en) Micro-pipettor apparatus
EP2010325B1 (fr) Procédé de sélection d'une pointe de pipette et dispositif permettant son application
WO2009114350A1 (fr) Dispositif d'aspiration dans une pipette
WO2014071260A1 (fr) Dispositifs en forme de crayon pour la préparation et l'analyse d'échantillons biologiques
US9180446B2 (en) Manual dosing device
TR202005922U5 (tr) (Yüksek Hacim aralıklı) fonksiyonel çok kanallı mikropipet
CN215029034U (zh) 一种基于称重的自动移液装置
GB2161398A (en) Pipette
CN113711056A (zh) 具有移液器适配性的集成微流控设备
CN214844205U (zh) 用于中药材检测的移液器
CN212955159U (zh) 在基于pcr的分子诊断装置中使用的化验盒
Ewald et al. Fundamentals of dispensing

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21720029

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 21720029

Country of ref document: EP

Kind code of ref document: A1