WO2021205151A3 - Systèmes et procédés de lyse cellulaire - Google Patents

Systèmes et procédés de lyse cellulaire Download PDF

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Publication number
WO2021205151A3
WO2021205151A3 PCT/GB2021/050822 GB2021050822W WO2021205151A3 WO 2021205151 A3 WO2021205151 A3 WO 2021205151A3 GB 2021050822 W GB2021050822 W GB 2021050822W WO 2021205151 A3 WO2021205151 A3 WO 2021205151A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell lysis
methods
lysis systems
ultrasonic transducer
lysis device
Prior art date
Application number
PCT/GB2021/050822
Other languages
English (en)
Other versions
WO2021205151A2 (fr
Inventor
Imad Lahoud
Jeff Machovec
Sajid BHATTI
Mohammed ALSHAIBA SALEH GHANNAM ALMAZROUEI
Clement Lamoureux
Original Assignee
Shaheen Innovations Holding Limited
RUSBY-GALE, Daniel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP20168245.7A external-priority patent/EP3834949A1/fr
Priority claimed from US16/889,667 external-priority patent/US11254979B2/en
Priority claimed from US17/122,025 external-priority patent/US11672928B2/en
Priority to GB2214906.6A priority Critical patent/GB2609770A/en
Priority to CA3174154A priority patent/CA3174154A1/fr
Priority to PE2022002187A priority patent/PE20230011A1/es
Priority to JP2022561050A priority patent/JP2023521340A/ja
Priority to KR1020227038658A priority patent/KR20230034202A/ko
Application filed by Shaheen Innovations Holding Limited, RUSBY-GALE, Daniel filed Critical Shaheen Innovations Holding Limited
Priority to BR112022020237A priority patent/BR112022020237A2/pt
Priority to AU2021252180A priority patent/AU2021252180A1/en
Priority to IL296991A priority patent/IL296991A/en
Priority to CN202180034196.4A priority patent/CN115701914A/zh
Priority to MX2022012456A priority patent/MX2022012456A/es
Priority to EP22207045.0A priority patent/EP4159053A1/fr
Priority to EP21716844.2A priority patent/EP4117462A2/fr
Priority to IL298682A priority patent/IL298682A/en
Priority to EP21731273.5A priority patent/EP4152967A1/fr
Priority to GB2219369.2A priority patent/GB2612211A/en
Priority to JP2022574097A priority patent/JP2023527574A/ja
Priority to PCT/GB2021/051332 priority patent/WO2021245390A1/fr
Priority to CA3180786A priority patent/CA3180786A1/fr
Priority to US17/334,461 priority patent/US11181451B1/en
Priority to IL298679A priority patent/IL298679A/en
Priority to JP2022574096A priority patent/JP2023527573A/ja
Priority to CA3180787A priority patent/CA3180787A1/fr
Priority to AU2021285406A priority patent/AU2021285406A1/en
Priority to GB2219365.0A priority patent/GB2611468A/en
Priority to KR1020227046452A priority patent/KR20230034987A/ko
Priority to AU2021285405A priority patent/AU2021285405A1/en
Priority to US17/334,531 priority patent/US11131000B1/en
Priority to PCT/GB2021/051333 priority patent/WO2021245391A1/fr
Priority to KR1020227046451A priority patent/KR20230034986A/ko
Priority to EP21731274.3A priority patent/EP4153354A1/fr
Priority to US17/466,877 priority patent/US11385148B2/en
Priority to US17/466,906 priority patent/US11274352B2/en
Publication of WO2021205151A2 publication Critical patent/WO2021205151A2/fr
Publication of WO2021205151A3 publication Critical patent/WO2021205151A3/fr
Priority to US17/693,209 priority patent/US11667979B2/en
Priority to US17/731,153 priority patent/US11946844B2/en
Priority to ZA2022/10963A priority patent/ZA202210963B/en
Priority to CONC2022/0015633A priority patent/CO2022015633A2/es
Priority to ZA2022/13088A priority patent/ZA202213088B/en
Priority to ZA2022/13089A priority patent/ZA202213089B/en
Priority to US18/203,584 priority patent/US11959146B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/0207—Driving circuits
    • B06B1/0223—Driving circuits for generating signals continuous in time
    • B06B1/0238—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave
    • B06B1/0246—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave with a feedback signal
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/0207—Driving circuits
    • B06B1/0223—Driving circuits for generating signals continuous in time
    • B06B1/0269—Driving circuits for generating signals continuous in time for generating multiple frequencies
    • B06B1/0284—Driving circuits for generating signals continuous in time for generating multiple frequencies with consecutive, i.e. sequential generation, e.g. with frequency sweep
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/06—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction
    • B06B1/0644—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction using a single piezoelectric element
    • B06B1/0651—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction using a single piezoelectric element of circular shape

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Un système de lyse cellulaire (1) comprend un appareil d'entraînement (2) et un dispositif de lyse cellulaire (3) qui peuvent être attachés de manière amovible l'un à l'autre. Le dispositif de lyse cellulaire (3) comprend un transducteur ultrasonore (12) et une chambre de sonification (11). L'appareil d'entraînement (2) entraîne le transducteur ultrasonore (12) pour émettre des ondes ultrasonores pour lyser des cellules dans un récipient d'échantillon (22) qui est transporté par le dispositif de lyse cellulaire (3).
PCT/GB2021/050822 2020-04-06 2021-04-01 Systèmes et procédés de lyse cellulaire WO2021205151A2 (fr)

Priority Applications (39)

Application Number Priority Date Filing Date Title
EP21716844.2A EP4117462A2 (fr) 2020-04-06 2021-04-01 Systèmes et procédés de lyse cellulaire
EP22207045.0A EP4159053A1 (fr) 2020-04-06 2021-04-01 Systèmes et procédés de lyse cellulaire
MX2022012456A MX2022012456A (es) 2020-04-06 2021-04-01 Sistemas y metodos de lisis celular.
CA3174154A CA3174154A1 (fr) 2020-04-06 2021-04-01 Systemes et procedes de lyse cellulaire
PE2022002187A PE20230011A1 (es) 2020-04-06 2021-04-01 Sistemas y metodos de lisis celular
JP2022561050A JP2023521340A (ja) 2020-04-06 2021-04-01 細胞溶解システム及び方法
KR1020227038658A KR20230034202A (ko) 2020-04-06 2021-04-01 세포 용해 시스템 및 방법
GB2214906.6A GB2609770A (en) 2020-04-06 2021-04-01 Cell lysis systems and methods
BR112022020237A BR112022020237A2 (pt) 2020-04-06 2021-04-01 Sistemas e métodos de lise celular
AU2021252180A AU2021252180A1 (en) 2020-04-06 2021-04-01 Cell lysis systems and methods
IL296991A IL296991A (en) 2020-04-06 2021-04-01 Systems and methods for cell lysis
CN202180034196.4A CN115701914A (zh) 2020-04-06 2021-04-01 细胞裂解系统和方法
KR1020227046451A KR20230034986A (ko) 2020-06-01 2021-05-28 감염증 스크리닝 시스템
EP21731274.3A EP4153354A1 (fr) 2020-06-01 2021-05-28 Dispositif de criblage de maladie infectieuse
CA3180787A CA3180787A1 (fr) 2020-06-01 2021-05-28 Dispositif de criblage de maladie infectieuse
US17/334,461 US11181451B1 (en) 2020-06-01 2021-05-28 Infectious disease screening system
PCT/GB2021/051333 WO2021245391A1 (fr) 2020-06-01 2021-05-28 Dispositif de criblage de maladie infectieuse
GB2219369.2A GB2612211A (en) 2020-06-01 2021-05-28 An infectious disease screening system
JP2022574097A JP2023527574A (ja) 2020-06-01 2021-05-28 感染症検査装置
PCT/GB2021/051332 WO2021245390A1 (fr) 2020-06-01 2021-05-28 Système de dépistage de maladie infectieuse
CA3180786A CA3180786A1 (fr) 2020-06-01 2021-05-28 Systeme de depistage de maladie infectieuse
IL298682A IL298682A (en) 2020-06-01 2021-05-28 Infectious disease screening device
IL298679A IL298679A (en) 2020-06-01 2021-05-28 Infectious disease screening system
JP2022574096A JP2023527573A (ja) 2020-06-01 2021-05-28 感染症検査システム
EP21731273.5A EP4152967A1 (fr) 2020-06-01 2021-05-28 Système de dépistage de maladie infectieuse
AU2021285406A AU2021285406A1 (en) 2020-06-01 2021-05-28 An infectious disease screening device
GB2219365.0A GB2611468A (en) 2020-06-01 2021-05-28 An infectious disease screening device
KR1020227046452A KR20230034987A (ko) 2020-06-01 2021-05-28 감염증 스크리닝 장치
AU2021285405A AU2021285405A1 (en) 2020-06-01 2021-05-28 An infectious disease screening system
US17/334,531 US11131000B1 (en) 2020-06-01 2021-05-28 Infectious disease screening device
US17/466,877 US11385148B2 (en) 2020-06-01 2021-09-03 Infectious disease screening system
US17/466,906 US11274352B2 (en) 2020-06-01 2021-09-03 Infectious disease screening device
US17/693,209 US11667979B2 (en) 2020-06-01 2022-03-11 Infectious disease screening device
US17/731,153 US11946844B2 (en) 2020-06-01 2022-04-27 Infectious disease screening system
ZA2022/10963A ZA202210963B (en) 2020-04-06 2022-10-05 Cell lysis systems and methods
CONC2022/0015633A CO2022015633A2 (es) 2020-04-06 2022-10-31 Sistemas y métodos de lisis celular
ZA2022/13088A ZA202213088B (en) 2020-06-01 2022-12-02 An infectious disease screening device
ZA2022/13089A ZA202213089B (en) 2020-06-01 2022-12-02 An infectious disease screening system
US18/203,584 US11959146B2 (en) 2020-06-01 2023-05-30 Infectious disease screening device

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
EP20168245.7A EP3834949A1 (fr) 2019-12-15 2020-04-06 Systèmes à générateur ultrasonique et méthodes
EP20168245.7 2020-04-06
US16/889,667 US11254979B2 (en) 2020-06-01 2020-06-01 Systems and devices for infectious disease screening
US16/889,667 2020-06-01
US202017065992A 2020-10-08 2020-10-08
US17/065,992 2020-10-08
US202063111592P 2020-11-09 2020-11-09
US63/111,592 2020-11-09
US17/122,025 2020-12-15
US17/122,025 US11672928B2 (en) 2019-12-15 2020-12-15 Mist inhaler devices

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US17/334,531 Continuation US11131000B1 (en) 2020-06-01 2021-05-28 Infectious disease screening device
US17/334,531 Continuation-In-Part US11131000B1 (en) 2020-06-01 2021-05-28 Infectious disease screening device
US17/334,461 Continuation-In-Part US11181451B1 (en) 2020-06-01 2021-05-28 Infectious disease screening system

Publications (2)

Publication Number Publication Date
WO2021205151A2 WO2021205151A2 (fr) 2021-10-14
WO2021205151A3 true WO2021205151A3 (fr) 2021-11-11

Family

ID=78022944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2021/050822 WO2021205151A2 (fr) 2020-04-06 2021-04-01 Systèmes et procédés de lyse cellulaire

Country Status (2)

Country Link
CL (1) CL2022002720A1 (fr)
WO (1) WO2021205151A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023147487A1 (fr) * 2022-01-28 2023-08-03 Plexbio Co., Ltd. Dispositif d'ultrasonication haute fréquence et ses utilisations

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0353365A2 (fr) * 1988-08-01 1990-02-07 Shigeru Chiba Dispositif de désagrégation de cellules par des ultrasons
US6100084A (en) * 1998-11-05 2000-08-08 The Regents Of The University Of California Micro-sonicator for spore lysis
EP1179585A2 (fr) * 1997-12-24 2002-02-13 Cepheid Cartouche de manipulation de fluide intégrée
US20040042936A1 (en) * 2002-08-28 2004-03-04 Koukichi Ido Cross-wave sonicator
US6878540B2 (en) * 1999-06-25 2005-04-12 Cepheid Device for lysing cells, spores, or microorganisms
US20180207551A1 (en) * 2012-03-15 2018-07-26 Flodesign Sonics, Inc. Driver and control for variable impedence load
WO2019130107A1 (fr) * 2017-12-28 2019-07-04 Ethicon Llc Détermination de l'état d'un effecteur d'extrémité ultrasonore

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0353365A2 (fr) * 1988-08-01 1990-02-07 Shigeru Chiba Dispositif de désagrégation de cellules par des ultrasons
EP1179585A2 (fr) * 1997-12-24 2002-02-13 Cepheid Cartouche de manipulation de fluide intégrée
US6100084A (en) * 1998-11-05 2000-08-08 The Regents Of The University Of California Micro-sonicator for spore lysis
US6878540B2 (en) * 1999-06-25 2005-04-12 Cepheid Device for lysing cells, spores, or microorganisms
US20040042936A1 (en) * 2002-08-28 2004-03-04 Koukichi Ido Cross-wave sonicator
US20180207551A1 (en) * 2012-03-15 2018-07-26 Flodesign Sonics, Inc. Driver and control for variable impedence load
WO2019130107A1 (fr) * 2017-12-28 2019-07-04 Ethicon Llc Détermination de l'état d'un effecteur d'extrémité ultrasonore

Also Published As

Publication number Publication date
CL2022002720A1 (es) 2023-09-01
WO2021205151A2 (fr) 2021-10-14

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