MX2022012456A - Sistemas y metodos de lisis celular. - Google Patents

Sistemas y metodos de lisis celular.

Info

Publication number
MX2022012456A
MX2022012456A MX2022012456A MX2022012456A MX2022012456A MX 2022012456 A MX2022012456 A MX 2022012456A MX 2022012456 A MX2022012456 A MX 2022012456A MX 2022012456 A MX2022012456 A MX 2022012456A MX 2022012456 A MX2022012456 A MX 2022012456A
Authority
MX
Mexico
Prior art keywords
cell lysis
methods
lysis systems
ultrasonic transducer
lysis device
Prior art date
Application number
MX2022012456A
Other languages
English (en)
Inventor
Imad Lahoud
Jeff Machovec
Sajid Bhatti
Saleh Ghannam Almazrouei Mohammed Alshaiba
Clement Lamoureux
Original Assignee
Shaheen Innovations Holding Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP20168245.7A external-priority patent/EP3834949A1/en
Priority claimed from US16/889,667 external-priority patent/US11254979B2/en
Priority claimed from US17/122,025 external-priority patent/US11672928B2/en
Application filed by Shaheen Innovations Holding Ltd filed Critical Shaheen Innovations Holding Ltd
Priority claimed from PCT/GB2021/050822 external-priority patent/WO2021205151A2/en
Publication of MX2022012456A publication Critical patent/MX2022012456A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/0207—Driving circuits
    • B06B1/0223—Driving circuits for generating signals continuous in time
    • B06B1/0238—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave
    • B06B1/0246—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave with a feedback signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/0207—Driving circuits
    • B06B1/0223—Driving circuits for generating signals continuous in time
    • B06B1/0269—Driving circuits for generating signals continuous in time for generating multiple frequencies
    • B06B1/0284—Driving circuits for generating signals continuous in time for generating multiple frequencies with consecutive, i.e. sequential generation, e.g. with frequency sweep
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/06—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction
    • B06B1/0644—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction using a single piezoelectric element
    • B06B1/0651—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction using a single piezoelectric element of circular shape

Abstract

Un sistema de lisis celular (1) comprende un aparato excitador (2) y un dispositivo de lisis celular (3) que se pueden conectar entre sí de manera desmontable. El dispositivo de lisis celular (3) comprende un transductor ultrasónico (12) y una cámara de sonicación (11). El aparato excitador (2) activa el transductor ultrasónico (12) para emitir ondas ultrasónicas para lisar las células en un recipiente de muestras (22) que es transportado por el dispositivo de lisis celular (3).
MX2022012456A 2020-04-06 2021-04-01 Sistemas y metodos de lisis celular. MX2022012456A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP20168245.7A EP3834949A1 (en) 2019-12-15 2020-04-06 Ultrasonic systems and methods
US16/889,667 US11254979B2 (en) 2020-06-01 2020-06-01 Systems and devices for infectious disease screening
US202017065992A 2020-10-08 2020-10-08
US202063111592P 2020-11-09 2020-11-09
US17/122,025 US11672928B2 (en) 2019-12-15 2020-12-15 Mist inhaler devices
PCT/GB2021/050822 WO2021205151A2 (en) 2020-04-06 2021-04-01 Cell lysis systems and methods

Publications (1)

Publication Number Publication Date
MX2022012456A true MX2022012456A (es) 2023-01-11

Family

ID=83902079

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022012456A MX2022012456A (es) 2020-04-06 2021-04-01 Sistemas y metodos de lisis celular.

Country Status (13)

Country Link
EP (2) EP4117462A2 (es)
JP (1) JP2023521340A (es)
KR (1) KR20230034202A (es)
CN (1) CN115701914A (es)
AU (1) AU2021252180A1 (es)
BR (1) BR112022020237A2 (es)
CA (1) CA3174154A1 (es)
CO (1) CO2022015633A2 (es)
GB (1) GB2609770A (es)
IL (1) IL296991A (es)
MX (1) MX2022012456A (es)
PE (1) PE20230011A1 (es)
ZA (1) ZA202210963B (es)

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2671135B2 (ja) * 1988-08-01 1997-10-29 東湘電機株式会社 細胞の超音波破砕装置
ES2309022T3 (es) * 1997-12-24 2008-12-16 Cepheid Dispositivo y procedimiento para lisis.
US6100084A (en) * 1998-11-05 2000-08-08 The Regents Of The University Of California Micro-sonicator for spore lysis
US6887693B2 (en) * 1998-12-24 2005-05-03 Cepheid Device and method for lysing cells, spores, or microorganisms
FR2791697B1 (fr) * 1999-04-01 2003-02-28 Biomerieux Sa Appareil et procede de lyse par ultrasons de cellules biologiques
US20040200909A1 (en) * 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
JP3092396U (ja) * 2002-08-28 2003-03-07 エレコン科学株式会社 交差超音波破砕器
CN1246448C (zh) * 2003-02-25 2006-03-22 清华大学 一种超声裂解细胞或剪切大分子装置及其使用方法
US8169122B1 (en) * 2006-12-15 2012-05-01 Dxna Llc Ultra sonic release of DNA or RNA
US10967298B2 (en) * 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9587236B2 (en) * 2013-01-18 2017-03-07 Folim G. Halaka Continuous sonication for biotechnology applications and biofuel production
JP7279051B2 (ja) * 2017-12-28 2023-05-22 エシコン エルエルシー 超音波エンドエフェクタの状態の決定

Also Published As

Publication number Publication date
BR112022020237A2 (pt) 2023-02-23
KR20230034202A (ko) 2023-03-09
ZA202210963B (en) 2024-02-28
EP4159053A1 (en) 2023-04-05
GB202214906D0 (en) 2022-11-23
CA3174154A1 (en) 2021-10-14
JP2023521340A (ja) 2023-05-24
IL296991A (en) 2022-12-01
CO2022015633A2 (es) 2023-05-29
EP4117462A2 (en) 2023-01-18
CN115701914A (zh) 2023-02-14
PE20230011A1 (es) 2023-01-09
GB2609770A (en) 2023-02-15
AU2021252180A1 (en) 2022-11-10

Similar Documents

Publication Publication Date Title
WO2021205151A3 (en) Cell lysis systems and methods
ZA202210963B (en) Cell lysis systems and methods
WO2022051657A9 (en) Portable ultrasound systems and methods
CN104568111B (zh) 一种医用水听器声压灵敏度及指向性测试方法
CN106691509A (zh) 一种超声波成像控制方法、装置及超声波成像仪
CN108648569A (zh) 一种含有控制系统的超声成像测井实验装置
CN105537091B (zh) 基于频率差拍式和声学参量阵技术的次声波合成方法及装置
Ko et al. Structure-Borne and Flow Noise Reductions (Mathematical Modeling)
Harvey et al. Simulation and measurement of nonlinear behavior in a high-power test cell
Larson et al. State switched acoustic source
CN106556459A (zh) 一种用于低频声源测试的双端面的力声互易装置和方法
Komori et al. Evaluation on flexible beamformers with curved-type parametric loudspeaker for spatial audible area design
CN104568112B (zh) 一种医用水听器声压灵敏度及指向性测试装置
RU2012100923A (ru) Устройство для измерения акустического сопротивления твердых материалов
Chen et al. Performance Test and Simulation on Broadband PAA Transducer for Pipeline Detection
Leroyer et al. Computation Fluid Dynamics based model for a better prediction of fluid forces acting on iceberg capsize
Mustonen et al. High-power and time-reversed ultrasound based on FEM simulations and experiments
CN204769467U (zh) 一种超声波传感节点装置
Prakash et al. Ultrasonic detection and signal response of polymers in aquatic environment: Using arduino microcontroller
Diana et al. Use of feature extraction algorithm in real time echo detection application development
Mellinger et al. Methods for automatic detection of mysticete calls
Greene Use of Acoustical Holography for the Imaging of the Sources of Radiated Sound on a Complex Vibrator
Matsumoto et al. Noise reduction combining microphones and piezoelectric Devices
CN111239245A (zh) 一种弱胶结未成岩水合物超声波测试系统及方法
Greene Use of Acoustical Holography for the Imaging of the Sources of Radiated Sound on a Complex Vibrator