PE20230011A1 - Sistemas y metodos de lisis celular - Google Patents

Sistemas y metodos de lisis celular

Info

Publication number
PE20230011A1
PE20230011A1 PE2022002187A PE2022002187A PE20230011A1 PE 20230011 A1 PE20230011 A1 PE 20230011A1 PE 2022002187 A PE2022002187 A PE 2022002187A PE 2022002187 A PE2022002187 A PE 2022002187A PE 20230011 A1 PE20230011 A1 PE 20230011A1
Authority
PE
Peru
Prior art keywords
cell lysis
methods
lysis systems
ultrasonic transducer
lysis device
Prior art date
Application number
PE2022002187A
Other languages
English (en)
Inventor
Imad Lahoud
Jeff Machovec
Sajid Bhatti
Saleh Ghannam Almazrouei Mohammed Alshaiba
Clement Lamoureux
Original Assignee
Shaheen Innovations Holding Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP20168245.7A external-priority patent/EP3834949A1/en
Priority claimed from US16/889,667 external-priority patent/US11254979B2/en
Priority claimed from US17/122,025 external-priority patent/US11672928B2/en
Application filed by Shaheen Innovations Holding Ltd filed Critical Shaheen Innovations Holding Ltd
Priority claimed from PCT/GB2021/050822 external-priority patent/WO2021205151A2/en
Publication of PE20230011A1 publication Critical patent/PE20230011A1/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/0207—Driving circuits
    • B06B1/0223—Driving circuits for generating signals continuous in time
    • B06B1/0238—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave
    • B06B1/0246—Driving circuits for generating signals continuous in time of a single frequency, e.g. a sine-wave with a feedback signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/0207—Driving circuits
    • B06B1/0223—Driving circuits for generating signals continuous in time
    • B06B1/0269—Driving circuits for generating signals continuous in time for generating multiple frequencies
    • B06B1/0284—Driving circuits for generating signals continuous in time for generating multiple frequencies with consecutive, i.e. sequential generation, e.g. with frequency sweep
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B06—GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS IN GENERAL
    • B06B—METHODS OR APPARATUS FOR GENERATING OR TRANSMITTING MECHANICAL VIBRATIONS OF INFRASONIC, SONIC, OR ULTRASONIC FREQUENCY, e.g. FOR PERFORMING MECHANICAL WORK IN GENERAL
    • B06B1/00—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency
    • B06B1/02—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy
    • B06B1/06—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction
    • B06B1/0644—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction using a single piezoelectric element
    • B06B1/0651—Methods or apparatus for generating mechanical vibrations of infrasonic, sonic, or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction using a single piezoelectric element of circular shape
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

Un sistema de lisis celular (1) comprende un aparato excitador (2) y un dispositivo de lisis celular (3) que se pueden conectar entre si de manera desmontable; el dispositivo de lisis celular (3) comprende un transductor ultrasonico (12) y una camara de sonicacion (11); el aparato excitador (2) activa el transductor ultrasonico (12) para emitir ondas ultrasonicas para lisar las celulas en un recipiente de muestras (22) que es transportado por el dispositivo de lisis celular (3).
PE2022002187A 2020-04-06 2021-04-01 Sistemas y metodos de lisis celular PE20230011A1 (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP20168245.7A EP3834949A1 (en) 2019-12-15 2020-04-06 Ultrasonic systems and methods
US16/889,667 US11254979B2 (en) 2020-06-01 2020-06-01 Systems and devices for infectious disease screening
US202017065992A 2020-10-08 2020-10-08
US202063111592P 2020-11-09 2020-11-09
US17/122,025 US11672928B2 (en) 2019-12-15 2020-12-15 Mist inhaler devices
PCT/GB2021/050822 WO2021205151A2 (en) 2020-04-06 2021-04-01 Cell lysis systems and methods

Publications (1)

Publication Number Publication Date
PE20230011A1 true PE20230011A1 (es) 2023-01-09

Family

ID=83902079

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2022002187A PE20230011A1 (es) 2020-04-06 2021-04-01 Sistemas y metodos de lisis celular

Country Status (13)

Country Link
EP (2) EP4117462A2 (es)
JP (1) JP2023521340A (es)
KR (1) KR20230034202A (es)
CN (1) CN115701914A (es)
AU (1) AU2021252180A1 (es)
BR (1) BR112022020237A2 (es)
CA (1) CA3174154A1 (es)
CO (1) CO2022015633A2 (es)
GB (1) GB2609770A (es)
IL (1) IL296991A (es)
MX (1) MX2022012456A (es)
PE (1) PE20230011A1 (es)
ZA (1) ZA202210963B (es)

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2671135B2 (ja) * 1988-08-01 1997-10-29 東湘電機株式会社 細胞の超音波破砕装置
ES2309022T3 (es) * 1997-12-24 2008-12-16 Cepheid Dispositivo y procedimiento para lisis.
US6100084A (en) * 1998-11-05 2000-08-08 The Regents Of The University Of California Micro-sonicator for spore lysis
US6887693B2 (en) * 1998-12-24 2005-05-03 Cepheid Device and method for lysing cells, spores, or microorganisms
FR2791697B1 (fr) * 1999-04-01 2003-02-28 Biomerieux Sa Appareil et procede de lyse par ultrasons de cellules biologiques
US20040200909A1 (en) * 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
JP3092396U (ja) * 2002-08-28 2003-03-07 エレコン科学株式会社 交差超音波破砕器
CN1246448C (zh) * 2003-02-25 2006-03-22 清华大学 一种超声裂解细胞或剪切大分子装置及其使用方法
US8169122B1 (en) * 2006-12-15 2012-05-01 Dxna Llc Ultra sonic release of DNA or RNA
US10967298B2 (en) * 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9587236B2 (en) * 2013-01-18 2017-03-07 Folim G. Halaka Continuous sonication for biotechnology applications and biofuel production
JP7279051B2 (ja) * 2017-12-28 2023-05-22 エシコン エルエルシー 超音波エンドエフェクタの状態の決定

Also Published As

Publication number Publication date
BR112022020237A2 (pt) 2023-02-23
KR20230034202A (ko) 2023-03-09
ZA202210963B (en) 2024-02-28
MX2022012456A (es) 2023-01-11
EP4159053A1 (en) 2023-04-05
GB202214906D0 (en) 2022-11-23
CA3174154A1 (en) 2021-10-14
JP2023521340A (ja) 2023-05-24
IL296991A (en) 2022-12-01
CO2022015633A2 (es) 2023-05-29
EP4117462A2 (en) 2023-01-18
CN115701914A (zh) 2023-02-14
GB2609770A (en) 2023-02-15
AU2021252180A1 (en) 2022-11-10

Similar Documents

Publication Publication Date Title
WO2018083193A3 (en) Method and system for studying biological cells
CL2022002720A1 (es) Sistemas y métodos de lisis celular
CY1124717T1 (el) Μεθοδοι, kit και συσκευη για επεκταση πληθυσμου κυτταρων
AR076325A1 (es) Sistema y metodo para la estimacion de la relacion entre la velocidad de compresion y la velocidad de corte (vp/vs) en una region distante de una perforacion
FR2791697B1 (fr) Appareil et procede de lyse par ultrasons de cellules biologiques
WO2017202932A3 (en) An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid
ES2493690T3 (es) Dispositivo y método para generar un haz de energía acústica desde un orificio de perforación y aplicaciones del mismo
WO2015056003A3 (en) Apparatus and method using ultrasounds for gas conversion
AR074371A1 (es) Metodo de ensayo por ultrasonico y aparato
PE20230011A1 (es) Sistemas y metodos de lisis celular
CY1116515T1 (el) Ρυμουλκο σκαφος
BR112017005346B8 (pt) Dispositivo transdutor e de chassi transdutor, e, sistema e método para emissão e recepção de sinal ultrassônico
ZA202309449B (en) Micro bubble generation method and generation device
AR117676A1 (es) Aparato y sistema para guiar un implemento de limpieza y método de uso de los mismos
CL2019000771A1 (es) Metodo, sistema y herramienta para determinar el espesor de pared de un objeto.
AR114220A1 (es) Procedimiento de análisis de una muestra de roca
Wang et al. Contribution of cavitation generation to shock wave sterilization effects in a narrow water chamber
GB664763A (en) Method of and apparatus for examining materials by ultrasonic irradiation
CN203630096U (zh) 一种用于铁磁材料的电磁超声信号激励装置
FR3097528B1 (fr) Drone équipé d’un capteur à ultrasons
WO2018025088A3 (en) Use of vibrationnal spectroscopy for dna content inspection
PE20230422Z (es) Dispositivo vibrador ultrasonico para acabados de construccion
JP2000214061A (ja) 硬質材料の剪断試験方法及び試験装置
Vatandoost The embryo production through maturation, fertilization and extracorporeal culture of oocytes in three stages IVM, IVF and IVC
Isono et al. A high-speed microscopic system for observation of bubble-cell interaction from a lateral direction