WO2021193700A1 - 呼吸器感作性の測定方法および呼吸器感作性測定試薬 - Google Patents

呼吸器感作性の測定方法および呼吸器感作性測定試薬 Download PDF

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Publication number
WO2021193700A1
WO2021193700A1 PCT/JP2021/012167 JP2021012167W WO2021193700A1 WO 2021193700 A1 WO2021193700 A1 WO 2021193700A1 JP 2021012167 W JP2021012167 W JP 2021012167W WO 2021193700 A1 WO2021193700 A1 WO 2021193700A1
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WO
WIPO (PCT)
Prior art keywords
arylalkylcarbonyl
cysteine
lysine
respiratory
test
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2021/012167
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English (en)
French (fr)
Japanese (ja)
Inventor
正晴 藤田
裕介 山本
利彦 笠原
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Corp
Original Assignee
Fujifilm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujifilm Corp filed Critical Fujifilm Corp
Priority to JP2022510591A priority Critical patent/JPWO2021193700A1/ja
Priority to EP21775201.3A priority patent/EP4130739A4/en
Publication of WO2021193700A1 publication Critical patent/WO2021193700A1/ja
Priority to US17/951,053 priority patent/US20230024686A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/24—Immunology or allergic disorders
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food

Definitions

  • the test substance may be, for example, water, an organic solvent compatible with water (eg, methanol, ethanol, acetonitrile, acetone, N, N dimethyl sulfoxide (DMSO)), or a mixture thereof, even if the molar concentration cannot be adjusted. It may be dissolved in a solvent (a mixed solvent of water and an organic solvent, or a mixed solvent of two or more kinds of organic solvents).
  • a solvent a mixed solvent of water and an organic solvent, or a mixed solvent of two or more kinds of organic solvents.
  • the test substance is preferably dissolved to a concentration of 0.01 mg / mL to 10 mg / mL, and is dissolved to a concentration of 0.5 mg / mL to 5 mg / mL. It is more preferable to dissolve the mixture so as to have a concentration of 0.1 mg / mL to 1 mg / mL.
  • a fluorescent substance since a fluorescent substance is used as a measurement target, it has excellent selectivity and is used as an analytical means for a very small amount. Since the fluorescence intensity is proportional to the concentration of the fluorescent substance, quantitative analysis can be performed by creating a calibration curve.
  • the fluorescence detector a commercially available detector can be used, and examples thereof include those manufactured by Shimadzu Corporation, Waters, Hitachi, Agilent Technologies, and Osaka Soda.
  • Example 2 The 5 types of respiratory sensitizers and 27 types of non-respiratory sensitizers shown in the table below were evaluated under normal ADRA (conditions for reacting NAC and NAL separately).

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plasma & Fusion (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
PCT/JP2021/012167 2020-03-25 2021-03-24 呼吸器感作性の測定方法および呼吸器感作性測定試薬 Ceased WO2021193700A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2022510591A JPWO2021193700A1 (https=) 2020-03-25 2021-03-24
EP21775201.3A EP4130739A4 (en) 2020-03-25 2021-03-24 METHOD FOR MEASUREMENT OF RESPIRATORY SENSITIZATION AND REAGENT FOR MEASUREMENT OF RESPIRATORY SENSITIZATION
US17/951,053 US20230024686A1 (en) 2020-03-25 2022-09-22 Method for measuring respiratory sensitization and respiratory sensitization measuring reagent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2020054061 2020-03-25
JP2020-054061 2020-03-25

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/951,053 Continuation US20230024686A1 (en) 2020-03-25 2022-09-22 Method for measuring respiratory sensitization and respiratory sensitization measuring reagent

Publications (1)

Publication Number Publication Date
WO2021193700A1 true WO2021193700A1 (ja) 2021-09-30

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PCT/JP2021/012167 Ceased WO2021193700A1 (ja) 2020-03-25 2021-03-24 呼吸器感作性の測定方法および呼吸器感作性測定試薬

Country Status (4)

Country Link
US (1) US20230024686A1 (https=)
EP (1) EP4130739A4 (https=)
JP (1) JPWO2021193700A1 (https=)
WO (1) WO2021193700A1 (https=)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001059102A (ja) 1999-07-02 2001-03-06 Seco Tools Ab 工具を製造する方法、工具及び装置
JP2011059102A (ja) 2009-08-13 2011-03-24 Fujifilm Corp 皮膚感作性測定試薬
JP2014037995A (ja) 2012-08-13 2014-02-27 Fujifilm Corp 皮膚感作性測定試薬
US20150111771A1 (en) * 2012-04-26 2015-04-23 Senzagen Ab Method for Identifying Agents Capable of Inducing Respiratory Sensitization and Array and Analytical Kits for Use in the Method
JP2018505655A (ja) * 2014-11-28 2018-03-01 センソシェン アクティエ ボラーグ 分析方法及び分析方法で使用するためのアレイ

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LU93401B1 (en) * 2016-12-27 2018-07-24 Luxembourg Inst Science & Tech List Three-dimensional in vitro lung model, process for preparing said model, and its use for determining and/or predicting the sensitizing effects of inhalable products
JP7104793B2 (ja) * 2018-08-31 2022-07-21 富士フイルム株式会社 皮膚感作性の測定方法および皮膚感作性測定試薬

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001059102A (ja) 1999-07-02 2001-03-06 Seco Tools Ab 工具を製造する方法、工具及び装置
JP2011059102A (ja) 2009-08-13 2011-03-24 Fujifilm Corp 皮膚感作性測定試薬
US20150111771A1 (en) * 2012-04-26 2015-04-23 Senzagen Ab Method for Identifying Agents Capable of Inducing Respiratory Sensitization and Array and Analytical Kits for Use in the Method
JP2014037995A (ja) 2012-08-13 2014-02-27 Fujifilm Corp 皮膚感作性測定試薬
JP2018505655A (ja) * 2014-11-28 2018-03-01 センソシェン アクティエ ボラーグ 分析方法及び分析方法で使用するためのアレイ

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ARTS, J.: "How to assess respiratory sensitization of low molecular weight chemicals?", INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, vol. 225, no. 113469, 12 February 2020 (2020-02-12), pages 1 - 8, XP086072793, DOI: 10.1016/j.ijheh.2020.113469 *
DIK SANDER, RORIJE EMIEL, SCHWILLENS PAUL, VAN LOVEREN HENK, EZENDAM JANINE: "Can the Direct Peptide Reactivity Assay Be Used for the Identification of Respiratory Sensitization Potential of Chemicals?", TOXICOLOGICAL SCIENCES, ACADEMIC PRESS, vol. 153, no. 2, 1 October 2016 (2016-10-01), pages 361 - 371, XP055862489, ISSN: 1096-6080, DOI: 10.1093/toxsci/kfw130 *
HEMMING JOCELYN D. C., HOSFORD MARK, SHAFER MARTIN M.: "Application of the direct peptide reactivity assay (DPRA) to inorganic compounds: a case study of platinum species", TOXICOLOGY RESEARCH, vol. 8, no. 6, 1 November 2019 (2019-11-01), pages 802 - 814, XP055862495, DOI: 10.1039/c9tx00242a *
LALKO JFKIMBER IGERBERICK GFFOERTSCH LMAPI AMDEARMAN RJ: "The direct peptide reactivity assay: selectivity of chemical respiratory allergens", TOXICOLOGICAL SCIENCES, vol. 129, no. 2, 2012, pages 421 - 431, XP055862461, DOI: 10.1093/toxsci/kfs205
LALKO JON F., KIMBER IAN, GERBERICK G. FRANK, FOERTSCH LESLIE M., API ANNE MARIE, DEARMAN REBECCA J.: "The Direct Peptide Reactivity Assay: Selectivity of Chemical Respiratory Allergens", TOXICOLOGICAL SCIENCES, ACADEMIC PRESS, vol. 129, no. 2, 1 October 2012 (2012-10-01), pages 421 - 431, XP055862461, ISSN: 1096-6080, DOI: 10.1093/toxsci/kfs205 *
MIZOGUCHI IOHASHI MCHIBA YHASEGAWA HXU MOWAKI TYOSHIMOTO T: "Prediction of chemical respiratory and contact sensitizers by OX40L expression in dendric cells using a novel 3D coculture system", FRONTIERS IN IMMUNOLOGY, vol. 8, 2017, pages 929
MIZOGUCHI IZURU, OHASHI MIO, CHIBA YUKINO, HASEGAWA HIDEAKI, XU MINGLI, OWAKI TOSHIYUKI, YOSHIMOTO TAKAYUKI: "Prediction of Chemical Respiratory and Contact Sensitizers by OX40L Expression in Dendritic Cells Using a Novel 3D Coculture System", FRONTIERS IN IMMUNOLOGY, vol. 8, XP055862499, DOI: 10.3389/fimmu.2017.00929 *
NORTH, C. M. ET AL.: "Developing a framework for assessing chemical respiratory sensitization: A workshop report", REGULATORY TOXICOLOGY AND PHARMACOLOGY, vol. 80, 7 July 2016 (2016-07-07), pages 295 - 309, XP029708785, DOI: 10.1016/j_yrtph. 2016.06.00 6 *
See also references of EP4130739A4

Also Published As

Publication number Publication date
JPWO2021193700A1 (https=) 2021-09-30
EP4130739A1 (en) 2023-02-08
EP4130739A4 (en) 2023-06-14
US20230024686A1 (en) 2023-01-26

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