WO2021190223A1

WO2021190223A1 - Sample pre-processing device for detection and use method therefor

Info

Publication number: WO2021190223A1
Application number: PCT/CN2021/077445
Authority: WO
Inventors: 严义勇; 马红圳; 蔡振庆; 王炳志; 付辉
Original assignee: 深圳市易瑞生物技术股份有限公司
Priority date: 2020-03-27
Filing date: 2021-02-23
Publication date: 2021-09-30
Also published as: CN111298673A

Abstract

A sample pre-processing device for detection and a method using the sample pre-processing device. The sample pre-processing device comprises: guide rails (1), an initial position (2), a homogenizing and extracting position (3), a filtering and separating position (4), a liquid sucking and discharging position (5) and a collection position (6) being sequentially provided on the guide rails (1); and a base, used for supporting a fixing frame (10) comprising a plurality of holes; a lower portion of the base is connected to a bottom moving device to drive the base to move on the guide rails (1), such that the fixing frame can switch between the initial position (2), the homogenizing and extracting position (3), the filtering and separating position (4), and the liquid sucking and discharging position (5).

Description

A sample pre-processing equipment for testing and its use method

Technical field

The present invention relates to the field of biochemical detection, and more specifically to a sample pretreatment device for detection and a method of use thereof.

Background technique

In biochemical testing, in order to carry out the subsequent testing process normally, it is usually necessary to pre-process the sample. For solids, processes such as crushing, target extraction, and solid-liquid separation are required; for liquids, processes such as dilution or extraction and liquid-liquid separation are generally required as needed. At present, these inspections are generally independent steps, which are carried out by manual operation, and the process is cumbersome and takes up a lot of time and labor in the inspection process.

Therefore, there is a need for improved sample pretreatment equipment in this field.

Summary of the invention

In order to solve the above-mentioned problems, the present invention proposes a sample pre-processing device for testing, which solves the problem of cumbersome process in the sample processing process.

Therefore, in the first aspect, the present invention provides a sample pretreatment device for testing, including:

A guide rail, on which an initial position, a homogeneous extraction position, a filter separation position, a suction and discharge liquid level, and a collection position are sequentially arranged;

The base is used to support a fixed frame containing a plurality of holes; the lower part of the base is connected with a bottom moving device, which is used to drive the base to move on the guide rail to realize the fixed frame in the initial position and the uniform position. Switching between the mass extraction position, the filtered separation position and the suction and discharge liquid level;

A first fixing frame accommodating position is provided in the initial position, a first container is arranged on the first fixing frame, the first container includes a stirring rod, and an end cap is provided on the stirring rod;

The homogeneous extraction position is provided with a first vertical movement module. The first vertical movement module includes a first vertical movement motor, a motor plate, and a stirring motor and a biting head fixed on the motor plate. A vertical moving motor drives the motor board to move up and down, the biting head is used to engage with the end cap of the first container on the fixed frame, and the stirring motor drives the stirring rod in the first container to rotate;

The filter separation position is provided with a second vertical movement module, the second vertical movement module includes a second vertical movement motor, a push plate and a push rod fixed on the push plate, the second vertical movement The moving motor drives the pushing plate to move up and down;

The suction and discharge liquid level is provided with a third vertical movement module. The third vertical movement module includes a third vertical movement motor, a needle plate and a blowing needle fixed on the needle plate. The vertical moving motor drives the needle plate to move up and down, and the blowing needle is used to suck or release liquid;

The collection position is provided with a second fixing frame accommodating position, and the second fixing frame is used to place a second container of the processed sample;

The needle plate is movable between the suction and discharge liquid level and the collection position, so that the needle injects the liquid therein into the second container of the processed sample.

In one embodiment, the sample pre-processing equipment includes a washing tank and/or a sample rack placed on the fixed rack.

In one embodiment, there is a filter screen in the first container, the filter screen can be slidably mounted on the stirring rod, and the push rod pushes the filter screen down when it moves downward.

In one embodiment, the first container is a homogenizing cup.

In one embodiment, the second container is a test tube.

In one embodiment, the base slides on the guide rail.

In one embodiment, the occlusal head is provided with a ramp, a curved arc, and a right-angled surface.

In one embodiment, the first container is a bottle or tube for loading samples.

In one embodiment, the needle plate slides between the aspiration liquid level and the collection position.

In one embodiment, the fixing frame is used to fix the first container and the second container of different specifications, and the fixed part is provided with a movable mechanism. The fixation prevents the container body from rotating, vibrating and leaving its position during use.

In a second aspect, the present invention provides a sample pretreatment method for testing, the method uses the sample pretreatment device for testing of the first aspect of the present invention, and the method includes:

(1) Place a first fixing frame on the base of the initial position, and place a first container of the sample to be processed on the first fixing frame;

(2) The base moves on the guide rail to transport the fixed frame to the homogeneous extraction position, the first vertical moving motor drives the motor plate to move downward, and the bite head and the first container on the fixed frame The end cap bites and drives the stirring shaft to move. After the stirring is completed, the bite head and the end cap are released from bite, and the first vertical moving motor drives the motor board to return to position;

(3) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level, the third vertical movement motor drives the needle plate to go down, and the buffer is injected, and then the third vertical movement motor drives the needle plate Upward homing;

(4) The base moves on the guide rail to transport the fixed frame to the filter separation position. The second vertical moving motor drives the push plate down to push down the filter screen in the first container, and then the second vertical Drive the push plate to return to the position by the moving motor;

(5) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level. The third vertical movement motor drives the needle plate to go down and suck the liquid, and then the third vertical movement motor drives the needle plate to return to its position. ；

(6) Move the needle plate to the collection position, and spit out the liquid into the second container on the second fixed frame of the collection position.

In one embodiment, the fixed rack is placed in a washing tank or a sample rack.

In one embodiment, the base slides on the guide rail.

In one embodiment, the first container is a homogenizing cup.

In one embodiment, the second container is a test tube.

In one embodiment, the base slides on the guide rail.

In one embodiment, the first container is a bottle or tube for loading samples.

The sample pre-processing equipment for detection of the present invention can realize the functions of homogenization, sample extraction liquid separation, extraction liquid transfer and the like, and the sample can be directly used for instrument equipment or quick inspection detection and analysis after processing.

Description of the drawings

The present invention is illustrated by the following drawings

1A and 1B are schematic diagrams of the overall structure of a sample pretreatment device for testing according to an embodiment of the present invention, showing a top view (1A) and a side view (1B).

2A-2C are schematic diagrams of the structure of the motor plate (2A), the needle plate (2B) and the push plate (2C) of the sample pre-processing equipment for testing according to an embodiment of the present invention.

Figures 3A and 3B show schematic structural views of a homogenizing cup (3A) and a bite head (3B) according to an embodiment of the present invention.

4A and 4B show a schematic structural view of a cleaning station according to an embodiment of the present invention, and are shown as a top view (4A) and a side view (4B).

Detailed ways

Hereinafter, embodiments of the present invention will be described with reference to the drawings. The following detailed description and drawings are used to exemplarily illustrate the principle of the present invention. The present invention is not limited to the described preferred embodiments, and the scope of the application is defined by the claims. The present invention will now be described in detail with reference to exemplary embodiments, some of which are illustrated in the accompanying drawings. The following description is made with reference to the accompanying drawings, and unless otherwise indicated, the same reference numerals in different drawings represent the same or similar elements. The solutions described in the following exemplary embodiments do not represent all the solutions of the present invention. On the contrary, these solutions are merely examples of the devices and methods of the various aspects of the present invention referred to in the appended claims.

1A and 1B are schematic diagrams of the overall structure of a sample pretreatment device for testing according to an embodiment of the present invention, FIG. 1A shows a top view, and FIG. 1B shows a side view. The sample pre-processing equipment for testing includes: a guide rail 1 and a base. The guide rail 1 is sequentially provided with an initial position 2, a homogeneous extraction position 3, a filter separation position 4, a suction and discharge liquid level 5, and a collection position 6; the base is used to support a fixing frame 10 containing a plurality of holes; The lower part of the base is connected with a bottom moving device, which is used to drive the base to move on the guide rail 1. The guide rail can be similar to a railway track to realize the fixed rack 10 in the sample rack initial position 2, the homogeneous extraction position 3, the filtering separation position 4 and the suction Switch between the spit level 5; the base can slide on the guide rail 1.

The initial position 2 is used to place the first fixing frame 10, and the first fixing frame 10 is used to place the first container of the sample to be processed. The fixing rack 10 can be used to place a cleaning tank or a sample rack. For example, a plurality of holes on the fixing rack 10 are used to place bottles or tubes for loading samples. Preferably, the fixing frame 10 can be used to fix the first container and the second container of different specifications, and the fixed part is provided with a movable mechanism, and the side piece is drawn closer to the center piece by a spring, so as to realize the connection between the first container and the second container. Fixed to prevent the container body from rotating, vibrating and leaving the position during use. In the present invention, the movable mechanism uses a spring to pull the side pieces closer to the center piece to realize the fixation of the first container and the second container, and prevent the container body from rotating, vibrating and leaving the position during use. There are bolt holes at the four corners for fixing on the base.

The homogeneous extraction position 3 is provided with a first vertical moving module 31. The first vertical moving module 31 includes a first vertical moving motor 310, a motor plate 312, and a stirring motor 313 fixed on the motor plate 312 and a biting head , The first vertical moving motor 310 drives the motor plate 312 to move up and down, and the biting head is used to bite with the end cap of the first container on the fixing frame 10. Preferably, the bite head is a replaceable piece. In the present invention, the occlusal head is provided with an inclined platform, a curved arc and a right-angled surface; the inclined platform and curved arc facilitate the occlusion of the occlusal head and the end cap. When the occlusal head is lowered and combined with the end cap, it can gradually fit together. In case the position is not right, you can gradually slide the alignment to increase the chance of bite. In addition, you can also correct the position of the end cap and the stirring rod below; the right-angled surface provides a strong point to push it to rotate when pushing.

The filter separation position 4 is provided with a second vertical moving module 41, the second vertical moving module 41 includes a second vertical moving motor 410, a pushing plate 411, and a push rod 412 fixed on the pushing plate 411 , The second vertical moving motor 410 drives the pressing plate 411 to move up and down.

The suction and discharge liquid level 5 is provided with a third vertical movement module 51, and the third vertical movement module 51 includes a third vertical movement motor 510, a needle plate 511, and a needle 512 fixed on the needle plate 511 , The third vertical movement motor 510 drives the needle plate 511 to move up and down; the collection position 6 is used to place a second fixing frame 61, and the second fixing frame 61 is used to place a second container of processed samples , The needle plate 511 can move between the suction and discharge liquid level 5 and the collection position 6. For example, the needle plate 511 slides between the suction and discharge liquid level 5 and the collection position 6. For example, the sliding frame 7 and the transverse motor 71 are included.

2A-2C are schematic diagrams of the structure of the motor plate, the needle plate and the push plate of the sample pre-processing equipment for testing according to an embodiment of the present invention. As shown in FIG. 2A, a stirring motor 313 and a biting head 314 are fixed on the motor plate 312. As shown in FIG. 2B, a needle 512 is fixed on the needle plate 511, and the upper end of the needle 512 is connected to the pipeline 513. The pipe 513 is used to pass liquid. As shown in FIG. 2C, a push rod 412 is fixed on the pressing plate 411.

3A and 3B show the structural schematic diagrams of the homogenization cup 8 and the bite head 314 as the first container according to an embodiment of the present invention. The homogenizing cup 8 is composed of a cup shell 81 and a stirring rod 82. The stirring rod 82 includes an end cap 821 for engaging with the biting head 314. The end cap 821 has a partially conical structure with a protruding plane on the cone. The protruding plane includes a protrusion 8211 that is curved in one direction. The protrusion 8211 Used to push the stirring shaft to move. The occlusal head 314 includes a recessed structure. The recessed structure includes a ramp 3141 for combining with the inclined surface 8212 of the end cap, and a further recessed structure for combining with the protrusion of the end cap. The structure includes a curved arc 3142. , So as to reduce the collision probability when combined with the end cap protrusion 8211, and at the same time, there is a right-angled surface 3143 for pushing the protrusion 8211 to rotate. The stirring rod 82 may be combined with the cup shell 81 or independent of each other, but the mouth of the cup shell 81 is not completely closed, and the push rod 412 can extend into the mouth of the cup shell 81. In a preferred embodiment, the stirring rod 82 and the cup shell 81 are independent of each other. The stirring rod 82 is placed in the cup shell 81, and is fixed in position by the bite head 314 during stirring, and extends into the cup shell 81. The push rod 412 is a cylinder. Put it deep into the cup shell 81.

Figures 4A and 4B show a schematic structural view of a cleaning tank according to an embodiment of the present invention (Figure 4A is a top view and Figure 4B is a side view). The cleaning tank 9 is used for cleaning the sample needle after adding or drawing liquid into the homogenization cup. When cleaning is needed, the needle is inserted into the needle port 91, and the liquid drive mechanism quickly spit out a large amount of liquid to the needle port. After the liquid exceeds the capacity of the needle port, it enters the diversion port 93 through the connecting groove 92 and is discharged through the external drain port 94. Exhaust, the needle is thus cleaned.

Example 1: Treatment of solid samples.

Add 6 grams of fish meat and 5 ml of acetonitrile containing 1% acetic acid into 10 homogenization cups, put the homogenization cup into the holder, place the holder on the base, and then put the base into the guide rail to start the device. The base moves on the guide rail to transport the fixed frame to the homogeneous extraction position. The first vertical movement module includes a first vertical movement motor. The head is in contact with the top cap on the top of the homogenizing cup, the biting head bites the top cap, and drives the stirring shaft to move, and the fish is crushed. Then, the first vertical moving motor drives the motor board to move back up and down.

The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level. The third vertical movement module includes a third vertical movement motor. The third vertical movement motor drives the needle plate to go down. The needle is injected with 5 ml of water. Then, the third vertical moving motor drives the needle board to travel.

The base moves on the guide rail to transport the fixed frame to the filter separation position, the second vertical movement module includes a second vertical movement motor, and the second vertical movement motor drives the pressing plate down, and the pressing plate is used The push rod pushes the strainer in the homogenizing cup down to 4 cm from the bottom. Then, the second vertical moving motor drives the pressing plate to return to its position.

The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level, and the third vertical movement motor drives the needle plate to go down, and the needle on the needle plate is used to suck 6 ml of liquid. Then, the third vertical moving motor drives the needle plate to move, and the needle plate moves to the collection position. The needle spit out 6 ml of liquid into the test tube on the fixed frame of the collection position. This liquid is required for the next analysis. Test solution.

Example 2: Treatment of liquid samples.

Add 10 ml of milk and 5 ml of aqueous solution containing 5% trifluoroacetic acid into 10 homogenization cups, put the homogenization cup into the holder, place the holder on the base, and then put it into the guide rail to start the equipment. The base moves on the guide rail to transport the fixed frame to the homogeneous extraction position. The first vertical movement module includes a first vertical movement motor. The head is in contact with the top cap on the top of the homogenizing cup, and the biting head bites the top cap and drives the stirring shaft to move. Then, the first vertical moving motor drives the motor board to move back up and down.

The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level. The third vertical movement module includes a third vertical movement motor. The third vertical movement motor drives the needle plate to go down. The needle is injected with 5 ml of HEPES buffer at pH 10. Then, the third vertical moving motor drives the needle plate to return to its position.

The base moves on the guide rail to transport the fixed frame to the filter separation position, the second vertical movement module includes a second vertical movement motor, and the second vertical movement motor drives the pressing plate down, and the pressing plate is used The push rod pushes the strainer in the homogenizing cup down to 5 cm from the bottom. Then, the second vertical moving motor drives the pressing plate to return to its position.

Claims

A sample pretreatment equipment for testing, including:

A guide rail, on which an initial position, a homogeneous extraction position, a filter separation position, a suction and discharge liquid level, and a collection position are sequentially arranged;

The base is used to support a fixed frame containing a plurality of holes; the lower part of the base is connected with a bottom moving device, which is used to drive the base to move on the guide rail to realize the fixed frame in the initial position and the uniform position. Switching between the mass extraction position, the filtered separation position and the suction and discharge liquid level;

A first fixing frame accommodating position is provided in the initial position, a first container is arranged on the first fixing frame, the first container includes a stirring rod, and an end cap is provided on the stirring rod;

The homogeneous extraction position is provided with a first vertical movement module. The first vertical movement module includes a first vertical movement motor, a motor plate, and a stirring motor and a biting head fixed on the motor plate. A vertical moving motor drives the motor board to move up and down, the biting head is used to engage with the end cap of the first container on the fixed frame, and the stirring motor drives the stirring rod in the first container to rotate;

The filter separation position is provided with a second vertical movement module, the second vertical movement module includes a second vertical movement motor, a push plate and a push rod fixed on the push plate, the second vertical movement The moving motor drives the pushing plate to move up and down;

The suction and discharge liquid level is provided with a third vertical movement module. The third vertical movement module includes a third vertical movement motor, a needle plate and a blowing needle fixed on the needle plate. The vertical moving motor drives the needle plate to move up and down, and the blowing needle is used to suck or release liquid;

The collection position is provided with a second fixing frame accommodating position, and the second fixing frame is used to place a second container of the processed sample;

The needle plate is movable between the suction and discharge liquid level and the collection position, so that the needle injects the liquid therein into the second container of the processed sample.
The sample pretreatment device according to claim 1, wherein the sample pretreatment device comprises a cleaning tank and/or a sample rack placed on the fixed rack.
The sample pretreatment device according to claim 1 or 2, wherein the first container has a filter screen, the filter screen is slidably mounted on the stirring rod, and the push rod moves the filter screen downwards. Push down.
The sample pretreatment device according to any one of claims 1 to 3, wherein the base slides on the guide rail.
The sample pretreatment device according to any one of claims 1 to 4, wherein the first container is a bottle or tube for loading a sample.
The sample pretreatment device according to any one of claims 1 to 4, wherein the first container is a homogenization cup.
The sample pretreatment device according to any one of claims 1 to 6, wherein the occlusal head is provided with an inclined table, a curved arc, and a right-angled surface.
7. The sample pretreatment device according to any one of claims 1-7, wherein the needle plate slides between the suction and discharge liquid level and the collection position.
The sample pre-processing equipment according to any one of claims 1-8, the fixing frame is used to fix the first container and the second container of different specifications, the fixed part is provided with a movable mechanism, and the side piece is pulled toward the center piece by a spring Recently, the fixation of the first container and the second container is realized, and the container body is prevented from rotating, vibrating and leaving the position during use.
A sample pretreatment method for testing, the method uses the sample pretreatment device according to any one of claims 1-9, and the method comprises:

(1) Place a first fixing frame on the base of the initial position, and place a first container of the sample to be processed on the first fixing frame;

(2) The base moves on the guide rail to transport the fixed frame to the homogeneous extraction position, the first vertical moving motor drives the motor plate to move downward, and the bite head and the first container on the fixed frame The end cap of the occluding, and driving the stirring shaft to move, after the completion of the stirring, the occlusal head and the end cap are disengaged, and the first vertical moving motor drives the motor board to return to position;

(3) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level, the third vertical movement motor drives the needle plate to go down, and the buffer is injected, and then the third vertical movement motor drives the needle plate Upward homing;

(4) The base moves on the guide rail to transport the fixed frame to the filter separation position, and the second vertical moving motor drives the push plate down to push down the filter screen in the first container, and then Two vertical moving motors drive the push plate to return to position;

(5) The base moves on the guide rail to transport the fixed frame to the suction and discharge liquid level. The third vertical movement motor drives the needle plate to go down and suck the liquid, and then the third vertical movement motor drives the needle plate to return to its position. ；

(6) Move the needle plate to the collection position, and spit out the liquid into the second container on the second fixed frame of the collection position.