WO2021187815A1 - Formulation microbienne pour améliorer la tolérance au stress environnemental des plantes, contenant la souche flavobactérium sp. tch3-2 en tant que principe actif, et son utilisation - Google Patents

Formulation microbienne pour améliorer la tolérance au stress environnemental des plantes, contenant la souche flavobactérium sp. tch3-2 en tant que principe actif, et son utilisation Download PDF

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Publication number
WO2021187815A1
WO2021187815A1 PCT/KR2021/003099 KR2021003099W WO2021187815A1 WO 2021187815 A1 WO2021187815 A1 WO 2021187815A1 KR 2021003099 W KR2021003099 W KR 2021003099W WO 2021187815 A1 WO2021187815 A1 WO 2021187815A1
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strain
tch3
plants
flavobacterium
environmental stress
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PCT/KR2021/003099
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English (en)
Korean (ko)
Inventor
이선우
최수연
정은주
백광열
이형주
김은진
최기혁
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동아대학교산학협력단
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Publication of WO2021187815A1 publication Critical patent/WO2021187815A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/20—Flavobacterium

Definitions

  • the present invention relates to a microbial preparation for enhancing environmental stress tolerance of plants containing the Flavobacterium genus TCH3-2 strain as an active ingredient and uses thereof.
  • chloroplast and mitochondrial enzymes are inactivated, protein synthesis is inhibited, already synthesized proteins are degraded, and cell membrane damage occurs. Drying stress due to lack of moisture is also a major factor in reducing crop productivity. Therefore, it is required to develop a method for improving the tolerance of plants in a high temperature or dry stress situation, which is emerging as a factor that inhibits crop productivity.
  • Korean Patent No. 0942228 discloses 'a plant disease control agent using Flavobacterium hercinium ipb-C313 strain'
  • Korean Patent No. 1182103 discloses 'Soil root bacterium Flavobacterium'.
  • Leeum strain GSE09 a composition for controlling plant anthrax, increasing yield, and promoting maturation, and a method for controlling anthrax using the same.
  • the 'microbial preparation for enhancing environmental stress tolerance of plants containing TCH3-2 strain of the genus Flavobacterium as an active ingredient and use thereof' of the present invention has not yet been disclosed.
  • the present invention has been derived by the above requirements, the present inventors Flavobacterium sp. TCH3-2 strain (Accession No.: KACC81154BP) pepper ( Capsicum annuum ) or tomato ( Lycopersicon esculentum ) Planting soil
  • Flavobacterium sp. TCH3-2 strain accesion No.: KACC81154BP
  • pepper Capsicum annuum
  • tomato Lycopersicon esculentum
  • the present invention provides a microbial preparation for enhancing environmental stress resistance of plants containing a Flavobacterium sp. TCH3-2 strain or a culture solution thereof as an active ingredient.
  • the present invention is a method for enhancing environmental stress resistance of plants, comprising the step of treating a plant, seed or plant planting soil with an effective amount of a Flavobacterium sp. TCH3-2 strain or a culture solution thereof.
  • the present invention provides a method for preparing a microbial agent for enhancing environmental stress resistance of plants, comprising the step of culturing a Flavobacterium sp. TCH3-2 strain.
  • the Flavobacterium sp. TCH3-2 strain is expected to greatly contribute to improving the productivity of crops because it enhances the tolerance of plants to high temperature and drying stress.
  • 1 is a result of confirming the degree of wilting of tomato leaves when water is given after treating a tomato with a Flavobacterium sp. TCH3-2 strain and treating dry stress conditions.
  • FIG. 2 is a photograph showing the recovery of tomatoes from drying stress when the Flavobacterium sp. TCH3-2 strain is treated on tomatoes and water is given after treatment with drying stress conditions.
  • the present invention provides a microbial preparation for enhancing environmental stress resistance of plants containing a Flavobacterium sp. TCH3-2 strain or a culture solution thereof as an active ingredient.
  • Flavobacterium TCH3-2 strain of the present invention was isolated from the rhizome soil of crops, and was deposited with the National Academy of Agricultural Sciences on November 20, 2015 (Accession No.: KACC92107P), and as of February 22, 2021 It was given an international accession number (accession number: KACC81154BP).
  • the environmental stress may be high temperature or drying stress, but is not limited thereto.
  • the high temperature may be 30 ⁇ 40 °C, preferably may be 36 °C, but is not limited thereto.
  • the microbial preparations for enhancing the environmental stress tolerance of plants according to the present invention are, for example, in the form of direct sprayable solutions, powders and suspensions or highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, scattering substances Or it may be prepared in granules, but is not limited thereto.
  • the microbial preparation for enhancing environmental stress tolerance of plants of the present invention may be formulated in various forms. Such formulations may be prepared, for example, by adding solvents and/or carriers. Often, inert additives and surface-active substances such as emulsifiers or dispersants are mixed into the formulation.
  • Suitable surface-active substances are aromatic sulfonic acids (eg lignosulfonic acid, phenol-sulfonic acid, naphthalene- and dibutylnaphthalenesulfonic acid), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, alkalis of fatty alcohol sulfates metals, alkaline earth metals, ammonium salts, sulfated hexa-, hepta- and octadecanols, salts of fatty alcohol glycol ethers, sulfonates naphthalene and derivatives thereof, condensates of formaldehyde, naphthalene or naphthalenesulfonic acids, phenols and formaldehyde Condensate, polyoxyethyleneoctyl phenol ether, ethoxylated isooctyl-, octyl- or nonyl
  • Suitable solid carrier materials are, in principle, all porous and agriculturally acceptable carriers such as mineral earths (such as silica, silica gel, silicates, talc, kaolin, limestone, lime, chalk, bowls, ocher, clays, Dolomite, diatomaceous earth, calcium sulphate, magnesium sulphate, magnesium oxide, ground synthetics), fertilizers (e.g. ammonium sulphate, ammonium phosphate, ammonium nitrate, urea), vegetable products (e.g. grain meal, bark meal, wood meal) and nut shell powder) or cellulose powder, but is not limited thereto.
  • the said solid carrier can also be used 1 type or in mixture of 2 or more types.
  • the microbial agent for promoting environmental stress tolerance of plants of the present invention can be mixed with a spreading agent and penetrant, or a surfactant to increase crop absorption and effect.
  • the present invention also provides an effective amount of a Flavobacterium sp. TCH3-2 strain (KACC81154BP) or a culture solution thereof to a plant, plant seed or plant planting soil.
  • Environmental stress tolerance of a plant comprising the step of provide a way to enhance it.
  • a method of enhancing the environmental stress tolerance of the plant it can be carried out by immersing or irrigating the plant with an effective amount of the Flavobacterium genus TCH3-2 strain or a culture solution thereof, that is, spraying.
  • an effective amount of the Flavobacterium genus TCH3-2 strain or its culture solution is poured into the soil around the plant, or the seeds can be soaked in an effective amount of the Flavobacterium genus TCH3-2 strain or its culture solution. have.
  • the 'effective amount' of the present invention is an amount sufficient to produce beneficial or desired results, and in order to increase the environmental stress resistance of plants, the Flavobacterium genus TCH3-2 strain or a culture solution thereof is uniformly diluted with water, followed by a power spreader It can be applied to plants and arable land using the same suitable spraying equipment.
  • the plant may be pepper ( Capsicum annuum ) or tomato ( Lycopersicon esculentum ), but is not limited thereto.
  • the present invention also provides a method for producing a microbial agent for enhancing environmental stress resistance of plants, comprising the step of culturing Flavobacterium sp. TCH3-2 strain (KACC81154BP).
  • the culturing method of the Flavobacterium sp. TCH3-2 strain and the preparation method of the microorganism preparation may use any method known in the art, and is not particularly limited to a specific method.
  • Example 1 the effect of the Flavobacterium sp. TCH3-2 strain (KACC81154BP) on the drying tolerance of tomatoes was confirmed.
  • the soil used was horticultural top soil for rich farmers, and 17g each was transferred to a 45 ⁇ 45 mm2 pot, and tomato seedlings were planted.
  • the tomato used is Hawaii 7996 variety, and the seeds were surface sterilized before use.
  • Triton X-100 and 70% ethanol solution For surface sterilization, put the seeds in 0.1% Triton X-100 and 70% ethanol solution and mix vigorously for 1 minute, then discard the solution, then 0.1 % Triton X-100 and 1% sodium hypochlorite (sodium hypochlorite) solution was immersed and strongly mixed, and then the solution was removed, and then rinsed sufficiently with sterile water 5 times or more.
  • TCH3-2 strain (1.35 ⁇ 10 9 CFU/ml) suspension was treated to 4 ⁇ 10 8 CFU per g of soil in tomatoes planted in soil, and control treated tomato seedlings only with sterilized water, and tomatoes grown for one more week were used for drying tolerance test. Drying stress observed the recovery trend of withered leaves by watering again for 5 days after not watering for 5 days in the growth room under the same conditions. The percentage of withered leaves per tomato individual by evaluating the number of withered leaves every day was recorded. The number of tomato plants used for each treatment was 10.
  • Tomatoes were irrigated with distilled water during the growing season, and immediately after drying stress, they were immersed in 200 ml each at two hour intervals, and after planting, they were immersed in 100-200 ml of distilled water once during the observation period after planting and stress treatment.
  • the wilting phenomenon progressed from the 4th day, and on the 5th day, 80% of the total leaves withered. It was not observed at all, and wilting was observed in 50% of all leaves on the 5th day, and from the 6th day of water irrigation, the wilting phenomenon gradually decreased in the TCH3-2 strain treated group, and decreased to less than 20% on the 10th day. , In the control not treated with the TCH3-2 strain, the wilting phenomenon was not recovered even after irrigation with water (FIG. 1).
  • Flavobacterium sp. Spicy AR-4 variety was used to test the high temperature resistance of red pepper by the TCH3-2 strain.
  • Red pepper can grow optimally in the temperature range of 25-28°C during the day and 18-22°C at night, and when it is over 30°C during the flowering period, it adversely affects flowering and fruiting.
  • the average maximum temperature and average minimum temperature of the month (month) in which dead plants were counted were 31.8°C and the average minimum temperature was 23.4°C, which was higher than the optimum temperature for growth. There was a very large difference compared with the optimum temperature for growth.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Dentistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • Pest Control & Pesticides (AREA)
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Abstract

La présente invention concerne: une formulation microbienne pour améliorer la tolérance au stress environnemental de plantes, la formulation microbienne contenant la souche TCH3-2 de flavobactérium sp. en tant que principe actif; et son utilisation. La souche TCH3-2 de Flavobacterium sp. portant le numéro d'accès KACC81154BP peut améliorer la tolérance des plantes à une haute température ou à un stress sec, et elle est donc prévue pour contribuer significativement à améliorer la productivité des cultures.
PCT/KR2021/003099 2020-03-19 2021-03-12 Formulation microbienne pour améliorer la tolérance au stress environnemental des plantes, contenant la souche flavobactérium sp. tch3-2 en tant que principe actif, et son utilisation WO2021187815A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2020-0034003 2020-03-19
KR1020200034003A KR102360511B1 (ko) 2020-03-19 2020-03-19 플라보박테리움 속 tch3-2 균주를 유효성분으로 함유하는 식물의 환경 스트레스 내성 증진용 미생물 제제 및 이의 용도

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WO2021187815A1 true WO2021187815A1 (fr) 2021-09-23

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019062756A (ja) * 2017-09-28 2019-04-25 積水化学工業株式会社 高温耐性改善用組成物及び植物体の生育方法

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KR100942228B1 (ko) 2009-11-10 2010-02-16 충청남도 플라보박테리움 헤르시니움 이피비-씨313 균주를 이용한 식물병 방제제

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019062756A (ja) * 2017-09-28 2019-04-25 積水化学工業株式会社 高温耐性改善用組成物及び植物体の生育方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Genome analysis of plant associated bacteria and comparative metagenome analysis of rhizosphere microbial community", FINAL REPORT, February 2015 (2015-02-01), Korea, pages 1 - 99, XP009530961 *
BAEK, KWANG YEOL: "Investigation of Plant-Growth Promoting Effect and Transcriptome Analysis of Novel Species of Flavobacterium in Tomato Rhizosphere", MASTER'S THESIS, December 2017 (2017-12-01), Busan, Korea, pages 1 - 44, XP009532041 *
KIM JEONG-EUN; WOO OG-GEUM; BAE YOOWON; KEUM HYE LIM; CHUNG SUNGLAN; SUL WOO JUN; LEE JAE-HOON: "Enhanced Drought and Salt Stress Tolerance inbyHYN0056", JOURNAL OF PLANT BIOLOGY, BOTANICAL SOCIETY OF KOREA, SEOUL, KR, vol. 63, no. 1, 1 February 2020 (2020-02-01), KR, pages 63 - 71, XP037049165, ISSN: 1226-9239, DOI: 10.1007/s12374-020-09236-8 *
KIM, JEONG-EUN: "Identification of New PGPR Contributing to Abiotic Stress Resistance in Arabidopsis thaliana", MASTER'S THESIS MAJOR IN BIOLOGY EDUCATION GRADUATE SCHOOL OF EDUCATION PUSAN NATIONAL UNIVERSITY, 1 January 2019 (2019-01-01), Korea, pages 1 - 57, XP009530959 *

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KR102360511B1 (ko) 2022-02-09

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