Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
  • A61K31/407—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with other heterocyclic ring systems, e.g. ketorolac, physostigmine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
  • A61K31/5375—1,4-Oxazines, e.g. morpholine
  • A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/54—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one sulfur as the ring hetero atoms, e.g. sulthiame
  • A61K31/542—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one sulfur as the ring hetero atoms, e.g. sulthiame ortho- or peri-condensed with heterocyclic ring systems
  • A61K31/545—Compounds containing 5-thia-1-azabicyclo [4.2.0] octane ring systems, i.e. compounds containing a ring system of the formula:, e.g. cephalosporins, cefaclor, or cephalexine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
  • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
  • A61P35/02—Antineoplastic agents specific for leukemia
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/04—Ortho-condensed systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00

Definitions

• SETD2 INHIBITORS AND RELATED METHODS AND USES, INCLUDING COMBINATION THERAPIES BACKGROUND OF THE INVENTION Field of the Invention [0001]
• the present disclosure provides SETD2 protein inhibitors, and methods, compositions, and kits for treating diseases, disorders, or conditions in a subject with a SETD2 protein inhibitor.
• the methods, compositions, and kits for treating diseases, disorders, or conditions further comprise a second therapeutic agent, wherein the second therapeutic agent comprises one or more glucocorticoid receptor agonists, one or more immunomodulatory drugs, one or more proteasome inhibitors, one or more Bcl-2 inhibitors, one or more pleiotropic pathway modulators, one or more XPO1 inhibitors, one or more histone deacetylase inhibitors, or one or more EZH2 inhibitors, or a combination thereof.
• HMTs histone methyltransferases
• the level of expression of a particular gene is influenced by the presence or absence of one or more methyl groups at a relevant histone site.
• the specific effect of a methyl group at a particular histone site persists until the methyl group is removed by a histone demethylase, or until the modified histone is replaced through nucleosome turnover.
• other enzyme classes can decorate DNA and histones with other chemical species, and still other enzymes can remove these species to provide control of gene expression.
• SETD2 is a human histone methyltransferase located at the cytogenic band p21.31 of chromosome 3 (3p21.31).
• SETD2 stands for Suppressor of variegation, Enhancer of zeste, and Trithorax domain containing 2.
• the SETD2 protein comprises three conserved functional domains: (1) the triplicate AWS-SET-PostSET domain; (2) a WW domain; and (3) a Set2-Rbp1 interacting ("SRI") domain. These three functional domains define the biological function of SETD2. See, Li, J. et al., Oncotarget 7:50719-50734 (2016). SETD2 is believed to be the single human gene responsible for the trimethylation of lysine 36 (Lys-36) of histone H3 (H3K36me3) using dimethylated Lys-36 (H3K36me2) as substrate. Edmunds, J.W.
• SETD2 loss-of-function point mutations in SETD2 were reported in patients with acute leukemia. Zhu, X. et al., Nature Genetics 46: 287-293 (2014). Mutations in SETD2 have also been reported in pediatric high-grade gliomas. Fontebasso, A.M. et al., Acta Neuropathol.125: 659-669 (2013).
• the present disclosure generally provides SETD2 protein inhibitors, and methods, compositions, and kits for treating diseases, disorders, or conditions in a subject with a SETD2 protein inhibitor and, optionally, a second therapeutic agent, wherein the second therapeutic agent comprises glucocorticoid receptor agonists, immunomodulatory drugs, proteasome inhibitors, Bcl-2 inhibitors, pleiotropic pathway modulators, XPO1 inhibitors, histone deacetylase inhibitors, or EZH2 inhibitors, or a combination thereof.
• the second therapeutic agent comprises glucocorticoid receptor agonists, immunomodulatory drugs, proteasome inhibitors, Bcl-2 inhibitors, pleiotropic pathway modulators, XPO1 inhibitors, histone deacetylase inhibitors, or EZH2 inhibitors, or a combination thereof.
• the present disclosure provides methods of treating diseases, disorders, or conditions, e.g., cancer, in a subject in need thereof with: [0007] (1) a therapeutically effective amount of a substituted indole represented by any one of Formulae I, II, II-A, III, III-A, IV, IV-A, IV-B, IV-C, IV-D, V, V-A, V-B, VI, VII, VII-A, VII-B, VII-C, VII-D, VII-E, VII-F, VII-G, VII-H, VIII, VIII-A, or VIII-B, or a compound of Table 1, or a compound of Table 1B, below, and the pharmaceutically acceptable salts and solvates thereof, collectively referred to herein as a "Compounds of the Disclosure;" and [0008] (2) a therapeutically effective amount of a second therapeutic agent, [0009] wherein the second therapeutic agent comprises one or more glucocorticoid receptor agonists, one or more immuno
• the present disclosure provides a Compound of the Disclosure for use in treating cancer, e.g., multiple myeloma, in a subject in need thereof, wherein the Compound of the Disclosure is to be administered to the subject in combination with a Second Therapeutic Agent.
• the present disclosure provides a Compound of the Disclosure for use in the manufacture of a medicament for treating cancer in a mammal, wherein the Compound of the Disclosure is to be administered to the subject in combination with a Second Therapeutic Agent.
• the present disclosure provides a kit comprising a Compound of the Disclosure and a Second Therapeutic Agent.
• the present disclosure provides a Compound of the Disclosure, i.e., substituted indole represented by any one of Formulae I, II, II-A, III, III-A, IV, IV- A, IV-B, IV-C, IV-D, V, V-A, V-B, VI, VII, VII-A, VII-B, VII-C, VII-D, VII-E, VII- F, VII-G, VII-H, VIII, VIII-A, or VIII-B, or a compound of Table 1, or a compound of Table 1B.
• the present disclosure provides a pharmaceutical composition comprising a Compound of the Disclosure and a pharmaceutically acceptable carrier.
• the present disclosure provides methods of treating diseases, disorders, or conditions, e.g., cancer, in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a Compound of the Disclosure.
• the present disclosure provides a Compound of the Disclosure for use in treating cancer, e.g., multiple myeloma, in a subject in need thereof.
• the present disclosure provides a Compound of the Disclosure for use in the manufacture of a medicament for treating cancer in a subject.
• Fig. 1 is a line graph showing the anti-proliferative activity of Cpd. No.15, tazemetostat (Taz), and lenalidomide (Len), and combinations thereof, in KMS-11 cells over the course of a 14-day co-treatment study.
• Fig. 2 is a bar graph showing the anti-proliferative activity of Cpd.
• Fig. 3 is a line graph showing the synergistic anti-proliferative activity of Cpd. No.15 in combination with pomalidomide in KMS-11 cells in 7-day co-treatment study.
• Fig. 4 is a table showing the Loewe Excess Model analysis of the Cpd. No.15 plus pomalidomide combination in KMS-11 cells in 7-day co-treatment study. DETAILED DESCRIPTION OF THE INVENTION I.
• the compound having Formula I is not N-(1-(1-(L- alanyl)piperidin-4-yl)ethyl)-7-methyl-1H-indole-2-carboxamide, N-((1r,4r)-4-(3- aminopropanamido)cyclohexyl)-7-methyl-1H-indole-2-carboxamide, or N-((1r,4r)-4- aminocyclohexyl)-7-methyl-1H-indole-2-carboxamide.
• Compounds of the Disclosure are compounds having Formula I, wherein: [0039] R 1a is selected from the group consisting of halogen, C 1 -C 6 alkyl, C 1 -C 6 alkoxy, C 3 -C 8 cycloalkyl, (hydroxy)C 1-6 alkyl, and (C 3 -C 6 cycloalkyl)C 1-6 alkyl; [0040] R 1b , R 1c , and R 1d are each independently selected from the group consisting of hydrogen, halogen, C 1 -C 6 alkyl, C 2 -C 6 alkenyl, (hydroxy)C 1 -C 6 alkyl, and C 1 -C 6 alkoxy; [0041] R 1e is selected from the group consisting of hydrogen and C1-C6 alkyl; [0042] G 1 is selected from the group consisting of optionally substituted C6-C10 aryl, optionally substituted 5- to 10-membered heteroaryl, optionally
• Compounds of the Disclosure are compounds having Formula I, wherein: [0046] R 1a is selected from the group consisting of halogen, C1-C3 alkyl, C1-C3 alkoxy, C3-C6 cycloalkyl, (hydroxy)C1-4 alkyl, and (C3-C6 cycloalkyl)C1-4 alkyl; [0047] R 1b , R 1c , and R 1d are each independently selected from the group consisting of hydrogen, halogen, C 1 -C 3 alkyl, C 2 -C 4 alkenyl, (hydroxy)C 1 -C 4 alkyl, and C 1 -C 3 alkoxy; [0048] R 1e is selected from the group consisting of hydrogen and C1-C3 alkyl; [0049] G 1 is selected from the group consisting of optionally substituted C 6 -C 10 aryl, optionally substituted 5- to 10-membered heteroaryl, optionally substituted 3- to 10-
• Compounds of the Disclosure are compounds having Formula I, wherein is a double bond, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula I, wherein R 1e is hydrogen, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula I, wherein R 1a is C 1 -C 3 alkyl, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula I, wherein G 2 is hydrogen, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula II: or a pharmaceutically acceptable salt or solvate thereof, wherein R 1d and G 1 are as defined in connection with Formula I.
• Compounds of the Disclosure are compounds having Formulae I or II, wherein R 1d is selected from the group consisting of hydrogen and fluoro, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula II-A: or a pharmaceutically acceptable salt or solvate thereof, wherein G 1 is as defined in connection with Formula II.
• Compounds of the Disclosure are compounds having Formulae I, II, or II-A, wherein G 1 is selected from the group consisting of optionally substituted C6-C10 aryl, optionally substituted 5- to 9-membered heteroaryl, optionally substituted 3- to 10-membered heterocyclo, optionally substituted C6-C8 cycloalkyl, (5- to 9-membered heteroaryl)C 1 -C 6 alkyl, (5- to 9-membered heteroaryl)(C 6-10 aryl)C 1 -C 4 alkyl, (5- to 9-membered heteroaryl heteroaryl)(C3-C6 cycloalkyl)C 1 -C 4 alkyl, and (C3-C6 cycloalkyl)C 1 -C 4 alkyl, or a pharmaceutically acceptable salt or solvate thereof.
• G 1 is selected from the group consisting of optionally substituted C6-C10 aryl, optionally substituted 5- to 9-membered hetero
• Compounds of the Disclosure are compounds having Formula III-A: wherein R 1d , R 2a , R 2b , R 2c , R 2d , and R 2e are as defined in connection with Formula III, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is an optionally substituted 3- to 10-membered heterocycle linked to the rest of the molecule through a nitrogen atom, e.g., R 2b is: and the like.
• R a1 is -NR 5a R 5b and R 5a and R 5b are independently selected from the group consisting of hydrogen and C 1 -C 4 alkyl. In another embodiment, R a1 is optionally substituted 4- to 10- membered heterocyclo. [0174] In another embodiment, Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -2, R 2b -2A, or R 2b -2b, or a pharmaceutically acceptable salt or solvate thereof. In another embodiment, R b1 is C 1 -C 4 alkyl.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -3, R 2b -3A, or R 2b -3B, or a pharmaceutically acceptable salt or solvate thereof.
• R c2 and R c3 are each hydrogen.
• R c4 is hydrogen.
• m is 1.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -4, or a pharmaceutically acceptable salt or solvate thereof.
• R d2 and R d3 are each hydrogen or fluoro.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -5, R 2b -5A, or R 2b -5B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -6, R 2b -6A, or R 2b -6B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -7, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -8, R 2b -8A, R 2b -8B, R 2b -8C, or R 2b -8D, or a pharmaceutically acceptable salt or solvate thereof.
• R h2 is selected from the group consisting of hydrogen and C 1 -C 3 alkyl.
• R h3 is hydrogen.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -9, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 10, R 2b -10A, R 2b -10B, R 2b -10C, and R 2b -10d, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 11, R 2b -11A and R 2b -11B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -12, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 13, R 2b -13A, R 2b -13B, R 2b -13C, R 2b -13D, R 2b -13E, and R 2b -13F, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -14, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -15, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 16, R 2b -16A and R 2b -16B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -17, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -18, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -19, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -20, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 21, R 2b -21A and R 2b -21B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III, wherein R 2b is selected from the group consisting of R 2b -22, R 2b -22A and R 2b -22B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -23, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -24, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -25, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 26, R 2b -26A and R 2b -26B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 27, R 2b -27A and R 2b -27B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is selected from the group consisting of R 2b - 28, R 2b -28A and R 2b -28B, or a pharmaceutically acceptable salt or solvate thereof.
• R 2b is selected from the group consisting of R 2b - 28, R 2b -28A and R 2b -28B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -29, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is R 2b -30, R 2b -30A, or R 2b -30B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2b is any one or more of the R 11a groups provided in connection with Formula IV, see below, or a pharmaceutically acceptable salt or solvate thereof.
• R 2b is any one or more of the R 11a groups provided in connection with Formula IV, see below, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 4c is C 1 -C 4 alkyl, or a pharmaceutically acceptable salt or solvate thereof.
• R 2d is selected from the group consisting of hydrogen, fluoro, and chloro, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2d is hydrogen, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula III or Formula III-A, wherein R 2d is fluoro, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula IV, wherein Z 4 is selected from the group consisting of -O- and -CH2-; or Z 4 is absent, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula IV, wherein: [0219] Z 4 is selected from the group consisting of -O- and -CH 2 -; or Z 4 is absent; [0220] Z 5 is selected from the group consisting of -CH 2 - and -CH 2 CH 2 -; [0221] R 13c is selected from the group consisting of alkyl, haloalkyl, alkoxy, (alkoxy)alkyl, (hydroxy)alkyl, (cyano)alkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted cycloalkyl, and optionally substituted heterocycle, and [0222] R 1d is as defined in connection with Formula I, or a
• Compounds of the Disclosure are compounds having Formula IV-A: , or a pharmaceutically acceptable salt or solvate thereof, wherein R 1d , R 11a , and Z 4 are as defined in connection with Formula IV.
• Compounds of the Disclosure are compounds having Formula IV-B: or a pharmaceutically acceptable salt or solvate thereof, wherein R 1d , R 11a , and Z 4 are as defined in connection with Formula IV.
• Compounds of the Disclosure are compounds having Formula IV-C: or a pharmaceutically acceptable salt or solvate thereof, wherein R 1d , R 11a , and Z 4 are as defined in connection with Formula IV.
• Compounds of the Disclosure are compounds having Formula IV-D: or a pharmaceutically acceptable salt or solvate thereof, wherein R 1d , R 11a , and Z 4 are as defined in connection with Formula IV.
• Compounds of the Disclosure are compounds having Formula IV, IV-A, IV-B, IV-C, or IV-D, wherein Z 4 is -C(R 28a )(R 28b )-; and R 28a and R 28b are independently selected from the group consisting of hydrogen, C 1 -C 4 alkyl, and fluoro, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula IV, IV-A, IV-B, IV-C, or IV-D, wherein Z 4 is -C(R 28a )(R 28b )-; R 28a is hydrogen; and R 28b is selected from the group consisting of C 1 -C 4 alkyl and fluoro, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula IV, IV-A, IV-B, IV-C, or IV-D, wherein Z 4 is -C(R 28a )(R 28b )-; and R 28a and R 28b are independently C 1 -C 4 alkyl, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula IV, IV-A, IV-B, IV-C, or IV-D, wherein Z 4 is selected from the group consisting of -O-, -CH 2 -, and -N(R 23 ), or Z 4 is absent, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein Z 4 is -CH 2 - , or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is an optionally substituted 3- to 10-membered heterocycle linked to the rest of the molecule through a nitrogen atom, e.g., R 11a is and the like.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is a substituted 4- to 14-membered heterocyclo selected from the group consisting of: [0239] R 12a is selected from the group consisting of hydrogen, C 1 -C 3 alkyl, (C 1 -C 4 alkoxy)C 1 -C 4 alkyl; and (hydroxy)C 1 -C 4 alkyl; [0240] R 13a is selected from the group consisting of C 1 -C 4 alkyl; amino; unsubstituted C3- C 6 cycloalkyl; substituted C 3 -C 6 cycloalkyl having one or two substituents independently selected from the group consisting of halo, hydroxy, C 1 -C 4 alkyl, amino, and (amino)C1- C4 alkyl; (C 1 -C 4 alkoxy)C 1 -C
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is a substituted 4- to 14-membered heterocyclo is selected from the group consisting of: or a pharmaceutically acceptable salt or solvate thereof.
• R 12a is selected from the group consisting of hydrogen and C 1 -C 3 alkyl;
• R 13a is C 1 -C 4 alkyl;
• R 13b is C 1 -C 4 alkyl, or a pharmaceutically acceptable salt or solvate thereof.
• R 12a is selected from the group consisting of hydrogen and methyl; R 13a is methyl; and R 13b is methyl, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is any one or more of the R 2b groups provided in connection with Formula III, see above, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein: [0260] R 11a is selected from the group consisting of: [0261] and R a1 , R a2 , R a3 , R a4 , R a5 , R b1 , R c1 , R c2 , R c3 , R c4 , m, R d1 , R d2 , R d3 , R e1 , R f1 , R g1 , R h1 , R h2 , R h3 , R h4 , R i1 , Z 1 , R j1 , R k1 , R k2 , r, Z 2 , R n3 , R o1 , R o2 , R o3 , R p1 , Z 3
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein: [0263] R 11a is selected from the group consisting of: [0264] and R a1 , R a5 , R b1 , R e1 , R f1 , R h1 , R h2 , R h3 , R k1 , R n3 , R s1 , R t1 , R w1 , R x1 , and R y1 are as defined in connection with Formula III; or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -1, R 11a -1A, R 11a - 1B, R 11a -1C, or R 11a -1D, or a pharmaceutically acceptable salt or solvate thereof.
• R a1 is -NR 5a R 5b .
• R a1 is -NR 5a R 5b and R 5a and R 5b are independently selected from the group consisting of hydrogen and C 1 -C 4 alkyl.
• R a1 is optionally substituted 4- to 10-membered heterocyclo.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -2, R 11a -2A, or R 11a -2b, or a pharmaceutically acceptable salt or solvate thereof.
• R b1 is C 1 -C 4 alkyl.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -3, R 11a -3A, or R 11a -3B, or a pharmaceutically acceptable salt or solvate thereof.
• R c2 and R c3 are each hydrogen.
• R c4 is hydrogen. In another embodiment, m is 1.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -4, or a pharmaceutically acceptable salt or solvate thereof.
• R d2 and R d3 are each hydrogen or fluoro.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -5, R 11a -5A, or R 11a -5B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -6, R 11a -6A, or R 11a -6B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -7, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -8, R 11a -8A, R 11a - 8B, R 11a -8C, or R 11a -8D, or a pharmaceutically acceptable salt or solvate thereof.
• R h2 is selected from the group consisting of hydrogen and C 1 -C 3 alkyl.
• R h3 is hydrogen.
• Compounds of the Disclosure are compounds any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -9, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -10, R 11a -10A, R 11a -10B, R 11a -10C, and R 11a -10d, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -11, R 11a -11A and R 11a -11B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -12, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -13, R 11a -13A, R 11a -13B, R 11a -13C, R 11a -13D, R 11a -13E, and R 11a -13F, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -14, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -15, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -16, R 11a -16A and R 11a -16B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -17, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -18, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -19, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -20, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -21, R 11a -21A and R 11a -21B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -22, R 11a -22A and R 11a -22B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -23, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -24, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -25, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -26, R 11a -26A and R 11a -26B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -27, R 11a -27A and R 11a -27B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is selected from the group consisting of R 11a -28, R 11a -28A and R 11a -28B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -29, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV, IV-A, IV-B, IV-C, or IV-D, wherein R 11a is R 11a -30, R 11a -30A, or R 11a -30B, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV-A, IV-B, IV-C, or IV-D, wherein: [0306] Z 4 is -CH 2 -; and [0307] R 11a is selected from the group consisting of:
• Compounds of the Disclosure are compounds having any one of Formulae IV-A, IV-B, IV-C, or IV-D, wherein: [0309] Z 4 is -CH2-; and [0310] R 11a is selected from the group consisting of: or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae IV-A, IV-B, IV-C, or IV-D, wherein: [0312] Z 4 is -CH 2 -; [0313] R 11a is: R 27a is selected from the group consisting of hydrogen, C 1 -C 4 alkyl, C 1 -C 4 haloalkyl, (C1- C 4 alkoxy)C 1 -C 4 alkyl, and (hydroxy)C 1 -C 4 alkyl, or a pharmaceutically acceptable salt or solvate thereof. In another embodiment, R 27a is methyl.
• Compounds of the Disclosure are compounds Formula V: wherein: [0315] R 14a is selected from the group consisting of optionally substituted alkyl and optionally substituted heteroaryl; [0316] R 14b is selected from the group consisting of optionally substituted alkyl, optionally substituted heteroaryl, optionally substituted aryl, optionally substituted heterocyclo, optionally substituted cycloalkyl, and carboxamido; and [0317] p is 0, 1, 2, or 3; or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula V-A: wherein R 1d , R 14a , R 14d , and p are as defined in connection with Formula V, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula V-B: wherein R 1d , R 14a , R 14d , and p are as defined in connection with Formula V, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae V, V-A, or V-B, wherein R 14b is selected from the group consisting of unsubstituted 5- to 10-membered heteroaryl; substituted 5- to 10-membered heteroaryl having one or two substituents independently selected from the group consisting of C 1 -C 4 alkyl and (C 3 -C 6 cycloalkyl)C 1 -C 4 alkyl; unsubstituted C 6 -C 10 aryl; substituted C 6 -C 10 aryl, having one or two substituents independently selected from the group consisting of C 1 -C 4 alkyl and (3- to 8-membered heterocyclo)C 1 -C 4 alkyl; unsubstituted 4- to 14- membered heterocyclo; substituted 4- to 14-membered heterocyclo having one or two substituents independently selected from the group consisting of hydroxy, amino, and C 1 - C4 alky
• Compounds of the Disclosure are compounds having any one of Formulae V, V-A, or V-B, wherein R 14b is selected from the group consisting of unsubstituted 5- or 6-membered heteroaryl; substituted 5- or 6-membered heteroaryl having one or two substituents independently selected from the group consisting of C 1 -C 4 alkyl and (C3-C6 cycloalkyl)C 1 -C 4 alkyl; unsubstituted phenyl; substituted phenyl, having one or two substituents independently selected from the group consisting of C 1 -C 4 alkyl and (3- to 8-membered heterocyclo)C 1 -C 4 alkyl; and unsubstituted C 3 -C 6 cycloalkyl, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae V, V-A, or V-B, wherein p is 0, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having any one of Formulae V, V-A, or V-B, wherein p is 1, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VI: wherein: [0343] R 19 is selected from the group consisting of unsubstituted 4- to 14-membered heterocyclo; and substituted 4- to 14-membered heterocyclo having one or two substituents independently selected from the group consisting of amino, hydroxy, and C 1 -C 4 alkyl; [0344] R 20 is selected from the group consisting of hydrogen, halo, and C 1 -C 4 alkyl; and [0345] q is 1, 2, or 3, or a pharmaceutically acceptable salt or solvate thereof. [0346] In another embodiment, Compounds of the Disclosure are compounds having Formula VI, wherein q is 1.
• Compounds of the Disclosure are compounds having Formula VII: [0348] wherein: [0349] R 11b is selected from the group consisting of C 1 -C 4 alkyl, halo, and C 1 -C 4 haloalkyl; and [0350] R 1d and R 11a are as defined in connection with Formula IV, or a pharmaceutically acceptable salt or solvate thereof. [0351] In another embodiment, Compounds of the Disclosure are compounds having Formula VII-A: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VII-B: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VII-C: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VII-D: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VII-E: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VII-F: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VII-G: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VII-H: wherein R 1d , R 11a , and R 11b are as defined in connection with Formula VII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VIII: wherein: [0360] R 30 is selected from the group consisting of hydrogen; C 1 -C 6 alkyl; unsubstituted C3-C6 cycloalkyl; substituted C3-C6 cycloalkyl having one or two substituents independently selected from the group consisting of halo, hydroxy, C 1 -C 4 alkyl, amino, and (amino)C 1 -C 4 alkyl; unsubstituted 4- to 14-membered heterocyclo; and substituted 4- to 14-membered heterocyclo having one or two substituents independently selected from the group consisting of amino, hydroxy, and C 1 -C 4 alkyl; -C(
• Compounds of the Disclosure are compounds having Formula VIII-A: wherein R 1d , R 30 , and u are as defined in connection with Formula VIII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds having Formula VIII-B: wherein R 1d , R 30 , and u are as defined in connection with Formula VIII, or a pharmaceutically acceptable salt or solvate thereof.
• Compounds of the Disclosure are compounds of Table 1, and the pharmaceutically acceptable salts or solvates thereof. The chemical names of the compounds of Table 1 were generated by Chemdraw ® Professional version 17.0.0.206.
• Compounds of the Disclosure are selected from the group consisting of Cpd. Nos. 1228, 1229, 1230, 1231, 1232, 1233, 1234 and 1235, and the pharmaceutically acceptable salts or solvates thereof.
• Compounds of the Disclosure are selected from the group consisting of Cpd. Nos. 15, 942, 1184, and 1232, and the pharmaceutically acceptable salts or solvates thereof.
• the Compound of the Disclosure is Cpd. No. 15.
• the Compound of the Disclosure is Cpd. No. 1228.
• the Compound of the Disclosure is Cpd. No. 1229.
• the Compound of the Disclosure is Cpd. No. 1230. In a non-limiting embodiment, the Compound of the Disclosure is Cpd. No. 1231. In a non-limiting embodiment, the Compound of the Disclosure is Cpd. No. 1232. In a non-limiting embodiment, the Compound of the Disclosure is Cpd. No. 1233. In a non-limiting embodiment, the Compound of the Disclosure is Cpd. No. 1234. In a non-limiting embodiment, the Compound of the Disclosure is Cpd. No. 1235. In a non-limiting embodiment, the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No.15.
• the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No.1228. In a non-limiting embodiment, the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No. 1229. In a non-limiting embodiment, the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No.1230. In a non-limiting embodiment, the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No. 1231. In a non-limiting embodiment, the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No. 1232.
• the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No. 1233. In a non-limiting embodiment, the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No. 1234. In a non-limiting embodiment, the Compound of the Disclosure is a pharmaceutically acceptable salt or solvate of Cpd. No.1235 Table 1
• the present disclosure encompasses the preparation and use of salts of the Compounds of the Disclosure, including non-toxic pharmaceutically acceptable salts.
• pharmaceutically acceptable addition salts include inorganic and organic acid addition salts and basic salts.
• the pharmaceutically acceptable salts include, but are not limited to, metal salts such as sodium salt, potassium salt, cesium salt and the like; alkaline earth metals such as calcium salt, magnesium salt and the like; organic amine salts such as triethylamine salt, pyridine salt, picoline salt, ethanolamine salt, triethanolamine salt, dicyclohexylamine salt, N,N'-dibenzylethylenediamine salt and the like; inorganic acid salts such as hydrochloride, hydrobromide, phosphate, sulphate and the like; organic acid salts such as citrate, lactate, tartrate, maleate, fumarate, mandelate, acetate, dichloroacetate, trifluoroacetate, oxalate, formate and the
• compositions refers to any salt, e.g., obtained by reaction with an acid or a base, of a Compound of the Disclosure that is physiologically tolerated in the target subject (e.g., a mammal, e.g., a human).
• Acid addition salts can be formed by mixing a solution of the particular Compound of the Disclosure with a solution of a pharmaceutically acceptable non-toxic acid such as hydrochloric acid, fumaric acid, maleic acid, succinic acid, acetic acid, citric acid, tartaric acid, carbonic acid, phosphoric acid, oxalic acid, dichloroacetic acid, or the like.
• Basic salts can be formed by mixing a solution of the compound of the present disclosure with a solution of a pharmaceutically acceptable non-toxic base such as sodium hydroxide, potassium hydroxide, choline hydroxide, sodium carbonate and the like.
• a pharmaceutically acceptable non-toxic base such as sodium hydroxide, potassium hydroxide, choline hydroxide, sodium carbonate and the like.
• the present disclosure encompasses the preparation and use of solvates of Compounds of the Disclosure. Solvates typically do not significantly alter the physiological activity or toxicity of the compounds, and as such may function as pharmacological equivalents.
• the term "solvate” as used herein is a combination, physical association and/or solvation of a compound of the present disclosure with a solvent molecule such as, e.g.
• solvate encompasses both solution-phase and isolatable solvates.
• Compounds of the Disclosure can be present as solvated forms with a pharmaceutically acceptable solvent, such as water, methanol, ethanol, and the like, and it is intended that the disclosure includes both solvated and unsolvated forms of Compounds of the Disclosure.
• solvate is a hydrate.
• a "hydrate” relates to a particular subgroup of solvates where the solvent molecule is water.
• Solvates typically can function as pharmacological equivalents. Preparation of solvates is known in the art. See, for example, M. Caira et al, J. Pharmaceut. Sci., 93(3):601-611 (2004), which describes the preparation of solvates of fluconazole with ethyl acetate and with water. Similar preparation of solvates, hemisolvates, hydrates, and the like are described by E.C. van Tonder et al., AAPS Pharm. Sci. Tech., 5(1):Article 12 (2004), and A.L.
• a typical, non-limiting, process of preparing a solvate would involve dissolving a Compound of the Disclosure in a desired solvent (organic, water, or a mixture thereof) at temperatures above 20°C to about 25°C, then cooling the solution at a rate sufficient to form crystals, and isolating the crystals by known methods, e.g., filtration. Analytical techniques such as infrared spectroscopy can be used to confirm the presence of the solvent in a crystal of the solvate. II.
• the therapeutic methods of the present disclosure comprise administering a therapeutically effective amount of a Compound of the Disclosure in combination with a therapeutically effective amount of a Second Therapeutic Agent.
• the term "Second Therapeutic Agent” as used herein comprises one or more glucocorticoid receptor agonists, one or more immunomodulatory drugs, one or more proteasome inhibitors, one or more Bcl-2 inhibitors, one or more pleiotropic pathway modulators, one or more XPO1 inhibitors, one or more histone deacetylase inhibitors, or one or more EZH2 inhibitors, or a combination thereof.
• the Second Therapeutic Agent comprises one compound from one drug class, i.e., one glucocorticoid receptor agonist, one immunomodulatory drug, one proteasome inhibitor, one Bcl-2 inhibitor, one pleiotropic pathway modulator, one XPO1 inhibitor, one histone deacetylase inhibitor, or one EZH2 inhibitor.
• one drug class i.e., one glucocorticoid receptor agonist, one immunomodulatory drug, one proteasome inhibitor, one Bcl-2 inhibitor, one pleiotropic pathway modulator, one XPO1 inhibitor, one histone deacetylase inhibitor, or one EZH2 inhibitor.
• the Second Therapeutic Agent comprises two different compounds from one drug class, e.g., two different glucocorticoid receptor agonists, e.g., dexamethasone and prednisone, two different immunomodulatory drugs, two different proteasome inhibitors, two different Bcl-2 inhibitors, two different pleiotropic pathway modulators, two different XPO1 inhibitors, two different histone deacetylase inhibitors, or two different EZH2 inhibitors.
• two different glucocorticoid receptor agonists e.g., dexamethasone and prednisone
• two different immunomodulatory drugs two different proteasome inhibitors
• Bcl-2 inhibitors two different pleiotropic pathway modulators
• two different XPO1 inhibitors two different histone deacetylase inhibitors
• two different EZH2 inhibitors two different EZH2 inhibitors.
• the Second Therapeutic Agent comprises three different compounds from one drug class, e.g., three different glucocorticoid receptor agonists, e.g., dexamethasone, prednisone, and methylprednisolone, three different immunomodulatory drugs, three different proteasome inhibitors, three different Bcl-2 inhibitors, three different pleiotropic pathway modulators, three different XPO1 inhibitors, three different histone deacetylase inhibitors, or three different EZH2 inhibitors.
• three different glucocorticoid receptor agonists e.g., dexamethasone, prednisone, and methylprednisolone
• three different immunomodulatory drugs three different proteasome inhibitors
• Bcl-2 inhibitors three different pleiotropic pathway modulators
• three different XPO1 inhibitors three different histone deacetylase inhibitors
• EZH2 inhibitors three different EZH2 inhibitors.
• the Second Therapeutic Agent comprises three different compounds from two drug classes, e.g., two different glucocorticoid receptor agonists, e.g., dexamethasone and prednisone, and one immunomodulatory drug; two different glucocorticoid receptor agonists and one proteasome inhibitor; and so on.
• the Second Therapeutic Agent comprises compounds from different drug classes, for example, a first compound from a first drug class and second compound from a second drug class, wherein the first drug class and the second drug class are different.
• the Second Therapeutic agent comprises an EZH2 inhibitor, e.g., tazemetostat, and an immunomodulatory drug, e.g., lenoliminide.
• EZH2 inhibitor e.g., tazemetostat
• an immunomodulatory drug e.g., lenoliminide.
• combinations of drug classes are provide in the following table: [0377]
• the Second Therapeutic Agent comprises three compounds from three different drug classes.
• the Second Therapeutic Agent comprises a first compound from a first drug class, and a second compound from a second drug class, and a third compound from a third drug class, wherein the first drug class, the second drug class, and the third drug class are different.
• combinations of drug classes are provide in the following table:
• glucocorticoid receptor agonist or "GR agonist” as used herein refers to a compound that activates the glucocorticoid receptor.
• Glucocorticoid receptor agonists and methods of administering glucocorticoid receptor agonists to a subject are known in the art. See, e.g., Pufall, M. A., Adv Exp Med Biol. 872:315–333 (2015).
• Exemplary glucocorticoid receptor agonists include, but are not limited to, dexamethasone, hydrocortisone, corticosterone, prednisolone, methylprednisolone, prednisone, triamcinolone, mapracorat, ciclesonide, and (20S)-protopanaxatriol.
• the glucocorticoid receptor agonist is prednisone.
• the glucocorticoid receptor agonist is dexamethasone.
• immunomodulatory drug refers to a compound that inhibits the production of tumour necrosis factor, interleukin 6, immunoglobulin G, and/or VEGF, andand/or co-stimulates T cells and NK cells, and/or increases interferon gamma and interleukin 2 production.
• Immunomodulatory drugs and methods of administering immunomodulatory drugs to a subject are known in the art.
• Exemplary immunomodulatory drugs include, but art not limited to, thalidomide, lenalidomide, and pomalidomide.
• the immunomodulatory drug is pomalidomide.
• proteasome inhibitor refers to a compound that blocks the action of proteasomes and thus prevents the degredation of pro-apoptotic factors such as p53 protein.
• proteasome inhibitors and methods of administering proteasome inhibitors to a subject are known in the art.
• Exemplary proteasome inhibitors include, but art not limited to, bortezomib, carfilzomib, and ixazomib. In one embodiment, the proteasome inhibitor inhibitor is bortezomib.
• Bcl-2 inhibitor refers to a compound that inhibits the anti-apoptotic Bcl-2 protein.
• Bcl-2 inhibitors and methods of administering Bcl-2 inhibitors to a subject are known in the art.
• Examplary Bcl-2 inhibitors include but are not limited to, navitoclax (ABT-263), ABT-737, Sabutoclax, AT-1019 (Gossypol), TW-37, venetoclax (ABT-199), obatoclax, HA14-1, A-1155463, A-1331852, and WEHI-539.
• the Bcl-2 inhibitor is venetoclax.
• the term "pleiotropic pathway modulator" as used herein refers to compound that binds to cereblon to promote protein degredation.
• Pleiotropic pathway modulators and methods of administering pleiotropic pathway modulators to a subject are known in the art. See, e.g., Hagner et al., Blood 126:779-789 (2017).
• a non- limiting exemplary pleiotropic pathway modulator is CC-122.
• the term "XPO1 inhibitor” as used herein refers to an inhibitor of exportin-1 (also known as chromosome region maintenance 1 protein homolog; CRM1).
• XPO1 inhibitors and methods of administering XPO1 inhibitors to a subject are known in the art. See, e.g., Wang and Liu, Stem Cell Invest 6:6 (2019).
• a non-limiting exemplary XPO1 inhibitor is selinexor.
• histone deacetylase inhibitor or "HD inhibitor” as used herein refers to a compound that inhibit histone deactylase enzymes. Histone deacetylase inhibitors and methods of administering histone deacetylase inhibitors to a subject are known in the art. See, e.g., Eckschlager et al., Int. J. Mol. Sci. 18:1414 (2017) doi:10.3390/ijms18071414.
• Exemplary histone deacetylase inhibitors include, but are not limited to, romidepsin, belinostat, panobinostat, and vorinostate.
• the histone deacetylase inhibitor is panobinostat.
• EZH2 inhibitor refers to a compound that inhibits the enhancer of zeste homolog 2 enzyme.
• EZH2 inhibitors and methods of administering EZH2 inhibitors to a subject are known in the art. See, e.g., Lue and Amengual, Curr Hematol Malig Rep 13:369–382 (2016).
• Exemplary EZH2 inhibitors include, but are not limited to, tazemetostat, EPZ011989, EPZ005687, GSK126, PF-06821497, and valemetostat.
• the EZH2 inhibitor is tazemetostat.
• CT Embodiment I A method of treating a subject in need thereof, the method comprising administering to the subject: (a) a therapeutically effective amount of a Compound of the Disclosure; and (b) a therapeutically effective amount of a Second Therapeutic Agent, wherein the Second Therapeutic Agent comprises a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a Bcl-2 inhibitor, a pleiotropic pathway modulator, a XPO1 inhibitor, a histone deacetylase inhibitor, or an EZH2 inhibitor, or a combination thereof; and the subject has cancer.
• CT Embodiment II A method of treating a subject in need thereof, the method comprising administering to the subject: (a) a therapeutically effective amount of a Compound of the Disclosure; and (b) a therapeutically effective amount of a Second Therapeutic Agent, wherein the Second Therapeutic Agent comprises a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a
• CT Embodiment II wherein the Second Therapeutic Agent comprises a glucocorticoid receptor agonist.
• CT Embodiment III The method of CT Embodiment II, wherein the glucocorticoid receptor agonist is dexamethasone.
• CT Embodiment IV The method of any one of CT Embodiments I-III, wherein the Second Therapeutic Agent comprises an immunomodulatory drug.
• CT Embodiment V The method of CT Embodiment IV, wherein the immunomodulatory drug is pomalidomide or lenalidomide.
• CT Embodiment VI The method of CT Embodiment VI.
• CT Embodiment VII The method of CT Embodiment VI, wherein the proteasome inhibitor is bortezomib.
• CT Embodiment VIII The method of any one of CT Embodiment I-VII, wherein the Second Therapeutic Agent comprises a Bcl-2 inhibitor.
• CT Embodiment IX The method of CT Embodiment VIII, wherein the Bcl-2 inhibitor is venetoclax.
• CT Embodiment X The method of any one of CT Embodiments I-IX, wherein the Second Therapeutic Agent comprises a pleiotropic pathway modulator.
• CT Embodiment XI The method of CT Embodiment X, wherein the pleiotropic pathway modulator is CC-122.
• CT Embodiment XII The method of any one of CT Embodiments I-XI, wherein the Second Therapeutic Agent comprises a XPO1 inhibitor.
• CT Embodiment XIII The method of CT Embodiment XII, wherein the XPO1 inhibitor is selinexor
• CT Embodiment XIV The method of any one of CT Embodiments I-XIII, wherein the Second Therapeutic Agent comprises a histone deacetylase inhibitor.
• CT Embodiment XV The method of any one of CT Embodiments I-XIII, wherein the Second Therapeutic Agent comprises a histone deacetylase inhibitor.
• CT Embodiment XIV wherein the histone deacetylase inhibitor is panobinostat.
• CT Embodiment XVI The method of any one of CT Embodiments I-XV, wherein the Second Therapeutic Agent is an EZH2 inhibitor.
• CT Embodiment XVII The method of CT Embodiment XVI, wherein the EZH2 inhibitor is tazemetostat. III.
• the present disclosure is directed generally to a method for treating a disease, condition, or disorder in a subject suffering from, or at risk of suffering from, the disease, condition, or disorder, the method comprising administering to the subject an effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent.
• the disease, condition, or disorder is responsive to or mediated by the inhibition of SETD2 protein by a Compound of the Disclosure.
• a Compound of the Disclosure can be administered to a subject having cancer as a single agent.
• a Compound of the Disclosure can also be administered to a subject having cancer in combination with a Second Therapeutic Agent.
• a Compound of the Disclosure and the Second Therapeutic Agent can be administered in combination under one or more of the following conditions: as separate pharmaceutical compositions, at different periodicities, e.g., simultaneously or sequentially, at different durations, at different concentrations, by different administration routes, etc. Additional optional therapeutic, e.g., anticancer, agents may also be administered to the cancer patient.
• the present disclosure is also directed to a method of inhibiting SETD2 protein in subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of a Compound of the Disclosure.
• the present disclosure provides a method of treating a disease, disorder, or condition in a subject comprising administering a therapeutically effective amount of a Compound of the Disclosure.
• the present disclosure provides a method of treating a disease, disorder, or condition in a subject comprising administering a therapeutically effective amount of a Compound of the Disclosure in combination with a Second Therapeutic Agent.
• the present disclosure provides a method of treating cancer in a subject comprising administering a therapeutically effective amount of a Compound of the Disclosure.
• the present disclosure provides a method of treating cancer in a subject comprising administering a therapeutically effective amount of a Compound of the Disclosure in combination with a Second Therapeutic Agent.
• Compounds of the Disclosure treat cancer by inhibiting SETD2 protein. Examples of treatable cancers include, but are not limited to, the cancers listed in Table 2. Table 2
• the cancer is pancreatic cancer or esophageal cancer.
• the cancer is selected from the group consisting of esophageal cancer, kidney cancer, stomach cancer, hepatocellular carcinoma, glioblastoma, central nervous system (CNS) cancer, soft tissue cancer, lung cancer, breast cancer, bladder/urinary tract cancer, head and neck cancer, prostate cancer, hematological cancer, pancreatic cancer, skin cancer, endometrial cancer, ovarian cancer, and colorectal cancer.
• the cancer or cancer cell is a hematological cancer. Exemplary hematological cancers include, but are not limited to, the cancers listed in Table 3.
• the cancer is multiple myeloma.
• the multiple myeloma is characterized as having chromosomal translocations involving the immunoglobulin heavy chain locus at 14q32.
• the chromosomal translocation is a t(4;14) translocation, i.e., the multiple myeloma is t(4;14) multiple myeloma.
• the cancer is mantle cell lymphoma.
• the cancer is diffuse large B-cell lymphoma.
• the present disclosure provides a therapeutic method of modulating protein methylation, gene expression, cell proliferation, cell differentiation and/or apoptosis in vivo in the cancers mentioned above by administering a therapeutically effective amount of a Compound of the Disclosure to a subject in need of such therapy and, optionally, a Second Therapeutic Agent.
• a Therapeutically effective amount of a Compound of the Disclosure to a subject in need of such therapy and, optionally, a Second Therapeutic Agent.
• the present disclosure provides the following particular embodiments relating to Compounds of the Disclosure, methods of treating cancer with a Compound of the Disclosure, and methods of treating cancer with a Compound of the Disclosure in combination with a Second Therapeutic Agent.
• Embodiment I Embodiment I.
• a method of treating a subject in need thereof comprising administering to the subject a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent, wherein the subject has cancer, and the Second Therapeutic Agent is selected from the group consisting of a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a Bcl-2 inhibitor, a pleiotropic pathway modulator, a XPO1 inhibitor, a histone deacetylase inhibitor, and an EZH2 inhibitor, or a combination thereof.
• Embodiment II The method of Embodiment I, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment III Embodiment III.
• Embodiment I wherein the cancer is a hematological cancer.
• Embodiment IV The method of Embodiment III, wherein the hematological cancer is any one or more of the cancers of Table 3, e.g., multiple myeloma.
• Embodiment V The method of Embodiment IV, wherein the hematological cancer is t(4;14) multiple myeloma.
• Embodiment VI The method of any one of Embodiments I-V further comprising administering a therapeutically effective amount of a Second Therapeutic Agent.
• Embodiment VII Embodiment VII.
• a pharmaceutical composition comprising a Compound of the Disclosure and a pharmaceutically acceptable carrier for use in treating cancer, wherein the pharmaceutical composition comprising a Compound of the Disclosure is, optionally, to be administered in combination with a Second Therapeutic Agent, wherein the Second Therapeutic Agent is selected from the group consisting of a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a Bcl-2 inhibitor, a pleiotropic pathway modulator, a XPO1 inhibitor, a histone deacetylase inhibitor, and an EZH2 inhibitor, or a combination thereof.
• Embodiment VIII The pharmaceutical composition of Embodiment VII, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment IX The pharmaceutical composition of Embodiment VII, wherein the cancer is a hematological cancer.
• Embodiment X The pharmaceutical composition of Embodiment IX, wherein the hematological cancer is any one or more of the cancers of Table 3, e.g., multiple myeloma.
• Embodiment XI The pharmaceutical composition of Embodiment X, wherein the hematological cancer is t(4;14) multiple myeloma.
• Embodiment XII The pharmaceutical composition of Embodiment X, wherein the hematological cancer is t(4;14) multiple myeloma.
• a Compound of the Disclosure for use in treatment of cancer wherein the Compound of the Disclosure is, optionally, to be administered in combination with a Second Therapeutic Agent, wherein the Second Therapeutic Agent is selected from the group consisting of a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a Bcl-2 inhibitor, a pleiotropic pathway modulator, a XPO1 inhibitor, a histone deacetylase inhibitor, and an EZH2 inhibitor, or a combination thereof.
• a glucocorticoid receptor agonist an immunomodulatory drug
• a proteasome inhibitor e.g., a Bcl-2 inhibitor
• a pleiotropic pathway modulator e.g., a XPO1 inhibitor
• a histone deacetylase inhibitor e.g., a histone deacetylase inhibitor
• EZH2 inhibitor e.g., EZH2 inhibitor
• Embodiment XII The compound for use of Embodiment XII, wherein the cancer is a hematological cancer.
• Embodiment XV The compound for use of Embodiment XIV, wherein the hematological cancer is any one or more of the cancers of Table 3, e.g., multiple myeloma.
• Embodiment XVI The compound for use of Embodiment XV, wherein the hematological cancer is t(4;14) multiple myeloma.
• Embodiment XVII Embodiment XVII.
• Embodiment XVIII The use of Embodiment XVII, wherein the cancer is any one or more of the cancers of Table 2. [0437] Embodiment XVIII.
• Embodiment XVII wherein the cancer is a hematological cancer.
• Embodiment XIX The use of Embodiment XVII, wherein the hematological cancer is any one or more of the cancers of Table 3, e.g., multiple myeloma.
• Embodiment XX The use of Embodiment XIX, wherein the hematological cancer is t(4;14) multiple myeloma.
• Embodiment XXI The use of any one of Embodiments XVII-XX for comprising a Secondary Therapeutic Agent.
• Embodiment XXII The use of any one of Embodiments XVII-XX for comprising a Secondary Therapeutic Agent.
• kits comprising a Compound of the Disclosure and, optionally, a Second Therapeutic Agent, and instructions for administering the Compound of the Disclosure to a subject having cancer.
• Embodiment XXIII The kit of Embodiment XXII, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment XXIV The kit of Embodiment XXII, wherein the cancer is a hematological cancer.
• Embodiment XXV Embodiment XXIV, wherein the hematological cancer is any one or more of the cancers of Table 3, e.g., multiple myeloma.
• Embodiment XXVI The kit of Embodiment XXV, wherein the hematological cancer is t(4;14) multiple myeloma.
• Embodiment XXVII The kit of any one of Embodiments XXII-XXVI further comprising a Secondary Therapeutic Agent.
• Embodiment XXVIII The method of Embodiment VI, wherein the Compound of the Disclosure and the Second Therapeutic agent are administered simultaneously.
• Embodiment XXIX The method of Embodiment VI, wherein the Compound of the Disclosure and the Second Therapeutic agent are administered sequentially.
• Embodiment XXXI The pharmaceutical composition of any one of Embodiments VII-XI, wherein the pharmaceutical composition is formulated for simultaneous administration of the Compound of the Disclosure and the Second Therapeutic Agent.
• Embodiment XXXI The pharmaceutical composition of any one of Embodiments VII-XI, wherein the pharmaceutical composition is formulated for sequential administration of the Compound of the Disclosure and the Second Therapeutic Agent.
• Embodiment XXXII The compound for use of any one of Embodiments XII-XVI, wherein the combination of the Compound of the Disclosure and the Second Therapeutic Agent is administered simultaneously.
• Embodiment XXXIII Embodiment XXIII.
• Embodiment XXXIV The use of any one of Embodiments XVII-XX, wherein the combination of the Compound of the Disclosure and the Second Therapeutic Agent is administered simultaneously.
• Embodiment XXXV The use of any one of Embodiments XVII-XX, wherein the combination of the Compound of the Disclosure and the Second Therapeutic Agent is administered sequentially.
• Embodiment XXXV The use of any one of Embodiments XVII-XX, wherein the combination of the Compound of the Disclosure and the Second Therapeutic Agent is administered sequentially.
• Embodiment 1. The use of any one of Embodiments XVII-XX, wherein the combination of the Compound of the Disclosure and the Second Therapeutic Agent is administered sequentially.
• Embodiment 2 The method of Embodiment 1, wherein the compound is a compound of Formula II: or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 3. The method of Embodiments 1 or 2, wherein G 1 is selected from the group consisting of: optionally substituted C 6 -C 10 aryl; optionally substituted 5- to 9-membered heteroaryl; optionally substituted 3- to 10-membered heterocyclo; optionally substituted C6-C8 cycloalkyl; (5- to 9-membered heteroaryl)C1-C6 alkyl; (5- to 9-membered heteroaryl)(C 6-10 aryl)C 1 -C 4 alkyl; (5- to 9-membered heteroaryl heteroaryl)(C 3 -C 6 cycloalkyl)C 1 -C 4 alkyl; and (C 3 -C 6 cycloalkyl)C 1 -C 4 alkyl, or a pharmaceutically acceptable salt
• Embodiment 5 The method of Embodiment 4, wherein the compound is a compound of Formula IV-A: IV-A, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 6. The method of Embodiment 4, wherein the compound is a compound of Formula IV-B: IV-B, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 7. The method of Embodiment 4, wherein the compound is a compound of Formula IV-C: IV-C, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 8. The method of Embodiment 4, wherein the compound is a compound of Formula IV-D: IV-D, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 10 The method of Embodiment 9, wherein R 11a is a substituted 4- to 14-membered heterocyclo selected from the group consisting of: [0513] R 12a is selected from the group consisting of hydrogen, C 1 -C 3 alkyl, (C 1 -C 4 alkoxy)C 1 -C 4 alkyl; and (hydroxy)C 1 -C 4 alkyl; [0514] R 13a is selected from the group consisting of C 1 -C 4 alkyl; amino; unsubstituted C3- C 6 cycloalkyl; substituted C 3 -C 6 cycloalkyl having one or two substituents independently selected from the group consisting of halo, hydroxy, C 1 -C 4 alkyl, amino, and (amino)C1- C4 alkyl; (C 1 -C 4 alkoxy)C 1 -C 4 alkyl; (hydroxy)C 1 -C 4 alkyl; unsubstituted
• R 27a and R 27b are each independently selected from the group consisting of hydrogen, C 1 -C 4 alkyl, C 1 -C 4 haloalkyl, (C 1 -C 4 alkoxy)C 1 -C 4 alkyl; and (hydroxy)C 1 -C 4 alkyl;
• R 27d is selected from the group consisting of hydrogen; C 1 -C 4 alkyl; and C 1 -C 4 haloalkyl; [0527] R 13b
• Embodiment 13 The method of Embodiment 9, wherein R 11a is a substituted 4- to 14-membered heterocyclo selected from the group consisting of: or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 14 The method of any one of Embodiments 4-13, wherein Z 4 is -CH2-, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 15 The method of any one of Embodiments 1-14, wherein R 1d is fluoro, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 1 The method of Embodiment 1, wherein the compound is a compound of Table 1, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 17 The method of Embodiment 1, wherein the compound is a compound of Table 1B, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 18 The method of any one of Embodiments 1-17, wherein the second therapeutic agent comprises a glucocorticoid receptor agonist.
• Embodiment 19 The method of Embodiment 18, wherein the glucocorticoid receptor agonist is dexamethasone.
• Embodiment 20 The method of Embodiment 1, wherein the compound is a compound of Table 1, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 21 The method of Embodiment 20, wherein the immunomodulatory drug is pomalidomide or lenalidomide.
• Embodiment 22 The method of any one of Embodiments 1-21, wherein the second therapeutic agent comprises a proteasome inhibitor.
• Embodiment 23 The method of Embodiment 22, wherein the proteasome inhibitor is bortezomib.
• Embodiment 24 The method of any one of Embodiments 1-23, wherein the second therapeutic agent comprises a Bcl-2 inhibitor.
• Embodiment 25 Embodiment 25.
• Embodiment 24 wherein the Bcl-2 inhibitor is venetoclax.
• Embodiment 26 The method of any one of Embodiments 1-25, wherein the second therapeutic agent comprises a pleiotropic pathway modulator.
• Embodiment 27 The method of Embodiment 26, wherein the pleiotropic pathway modulator is CC-122.
• Embodiment 28 The method of any one of Embodiments 1-27, wherein the second therapeutic agent comprises a XPO1 inhibitor.
• Embodiment 29 The method of Embodiment 28, wherein the XPO1 inhibitor is selinexor.
• Embodiment 30 The method of Embodiment 24, wherein the Bcl-2 inhibitor is venetoclax.
• Embodiment 31 The method of Embodiment 30, wherein the histone deacetylase inhibitor is panobinostat.
• Embodiment 32 The method of any one of Embodiments 1-31, wherein the second therapeutic agent is an EZH2 inhibitor.
• Embodiment 33 The method of Embodiment 32, wherein the EZH2 inhibitor is tazemetostat.
• Embodiment 34 The method of any one of Embodiments 1-33, wherein the compound of Formula I and the Second Therapeutic Agent are administered to the subject separately.
• Embodiment 35 Embodiment 35.
• Embodiment 36 The method of Embodiment 35, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment 37 The method of Embodiment 38, wherein the cancer is a hematological cancer.
• Embodiment 38 The method of Embodiment 37, wherein the hematological cancer is any one or more of the cancers of Table 3.
• Embodiment 39 The method of any one of Embodiments 1-34, wherein the subject in need thereof has cancer.
• Embodiment 40 The kit of Embodiment 39 further comprising instructions for administering the compound of Formula I and the Second Therapeutic Agent to a subject having cancer.
• Embodiment 41 A compound selected from the group consisting of: [0577] 4-fluoro-7-methyl-N-((1R,3S)-3-((3aS,6aS)-1-methyl-2- oxohexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl)cyclohexyl)-1H-indole-2-carboxamide; [0578] 4-fluoro-7-methyl-N-((1R,3R)-3-((3aS,6aS)-1-methyl-2- oxohexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl)cyclohexyl)-1H-indole-2-carboxamide; [0579] 4-fluoro-7-methyl-N-((1R,3S)-3-
• Embodiment 42 A pharmaceutical composition comprising the compound of Embodiment 41, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier.
• Embodiment 43 A method of treating a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the compound of Embodiment 41, or a pharmaceutically acceptable salt or solvate thereof.
• Embodiment 44 The method of Embodiment 43, wherein the subject in need thereof has cancer.
• Embodiment 45 The method of Embodiment 44, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment 46 is any one or more of the cancers of Table 2.
• Embodiment 44 wherein the cancer is a hematological cancer.
• Embodiment 47 The method of Embodiment 46, wherein the hematological cancer is any one or more of the cancers of Table 3.
• Embodiment 48 The pharmaceutical composition of Embodiment 43 for use in treating a subject.
• Embodiment 49 The pharmaceutical composition of Embodiment 48, wherein the subject has cancer.
• Embodiment 50 The pharmaceutical composition of Embodiment 49, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment 51 The pharmaceutical composition of Embodiment 49, wherein the cancer is a hematological cancer.
• Embodiment 52 The method of Embodiment 51, wherein the hematological cancer is any one or more of the cancers of Table 3.
• Embodiment 53 The compound of Embodiment 41 for use in treating a subject.
• Embodiment 54 The compound for use of Embodiment 53, wherein the subject has cancer.
• Embodiment 55 The compound for use of Embodiment 54, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment 56 The compound for use of Embodiment 54, wherein the cancer is a hematological cancer.
• Embodiment 57 The compound for use of Embodiment 54, wherein the cancer is a hematological cancer.
• Embodiment 56 wherein the hematological cancer is any one or more of the cancers of Table 3.
• Embodiment 58 Use of a compound of Embodiment 21 in the manufacture of a medicament for treating a subject.
• Embodiment 59 The use of Embodiment 58, wherein the subject has cancer.
• Embodiment 60 The use of Embodiment 59, wherein the cancer is any one or more of the cancers of Table 2.
• Embodiment 61 The use of Embodiment 59, wherein the cancer is a hematological cancer.
• Embodiment 62 The use of Embodiment 59, wherein the cancer is a hematological cancer.
• Embodiment 61 wherein the hematological cancer is any one or more of the cancers of Table 3.
• Embodiment 63 A kit comprising the compound of Embodiment 41 and instructions for administering the compound to a subject.
• Embodiment 64 The kit of Embodiment 63, wherein the subject cancer.
• Compounds of the Disclosure can be administered to a subject in the form of a raw chemical without any other components present.
• Compounds of the Disclosure can also be administered to a subject as part of a pharmaceutical composition containing the compound combined with a suitable pharmaceutically acceptable carrier.
• a suitable pharmaceutically acceptable carrier can be selected from pharmaceutically acceptable excipients and auxiliaries.
• compositions within the scope of the present disclosure include all compositions where a Compound of the Disclosure is combined with one or more pharmaceutically acceptable carriers. In one embodiment, the Compound of the Disclosure is present in the composition in an amount that is effective to achieve its intended therapeutic purpose. While individual needs may vary, a determination of optimal ranges of effective amounts of each compound is within the skill of the art.
• a Compound of the Disclosure can be administered to a mammal, e.g., a human, orally at a dose of from about 0.0025 to about 1500 mg per kg body weight of the mammal, or an equivalent amount of a pharmaceutically acceptable salt or solvate thereof, per day to treat the particular disorder.
• a useful oral dose of a Compound of the Disclosure administered to a mammal is from about 0.0025 to about 50 mg per kg body weight of the mammal, or an equivalent amount of the pharmaceutically acceptable salt or solvate thereof.
• the dose is typically about one-half of the oral dose.
• a unit oral dose may comprise from about 0.01 mg to about 1 g of the Compound of the Disclosure, e.g., about 0.01 mg to about 500 mg, about 0.01 mg to about 250 mg, about 0.01 mg to about 100 mg, 0.01 mg to about 50 mg, e.g., about 0.1 mg to about 10 mg, of the compound.
• the unit dose can be administered one or more times daily, e.g., as one or more tablets or capsules, each containing from about 0.01 mg to about 1 g of the compound, or an equivalent amount of a pharmaceutically acceptable salt or solvate thereof.
• a Compound of Disclosure or pharmaceutical composition comprising a Compound of the Disclosure and, optionally a Second Therapeutic Agent can be administered to any subject, e.g., a cancer patient in need thereof, that may experience the beneficial effects of a Compound of the Disclosure.
• a subject e.g., a cancer patient in need thereof.
• mammals e.g., humans and companion animals, although the disclosure is not intended to be so limited.
• the subject is a human.
• a pharmaceutical composition of the present disclosure can be administered by any means that achieves its intended purpose.
• administration can be by the oral, parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, transdermal, intranasal, transmucosal, rectal, intravaginal or buccal route, or by inhalation.
• the dosage administered and route of administration will vary, depending upon the circumstances of the particular subject, and taking into account such factors as age, gender, health, and weight of the recipient, condition or disorder to be treated, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect desired.
• a pharmaceutical composition of the present disclosure can be administered orally.
• a pharmaceutical composition of the present disclosure can be administered orally and is formulated into tablets, dragees, capsules, or an oral liquid preparation.
• the oral formulation comprises extruded multiparticulates comprising the Compound of the Disclosure.
• a pharmaceutical composition of the present disclosure can be administered rectally, and is formulated in suppositories.
• a pharmaceutical composition of the present disclosure can be administered by injection.
• a pharmaceutical composition of the present disclosure can be administered transdermally.
• a pharmaceutical composition of the present disclosure can be administered by inhalation or by intranasal or transmucosal administration.
• a pharmaceutical composition of the present disclosure can be administered by the intravaginal route.
• a pharmaceutical composition of the present disclosure can contain from about 0.01 to 99 percent by weight, e.g., from about 0.25 to 75 percent by weight, of a Compound of the Disclosure, e.g., about 1%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, or about 75% by weight of a Compound of the Disclosure.
• a pharmaceutical composition of the present disclosure is manufactured in a manner which itself will be known in view of the instant disclosure, for example, by means of conventional mixing, granulating, dragee-making, dissolving, extrusion, or lyophilizing processes.
• compositions for oral use can be obtained by combining the active compound with solid excipients, optionally grinding the resulting mixture and processing the mixture of granules, after adding suitable auxiliaries, if desired or necessary, to obtain tablets or dragee cores.
• Suitable excipients include fillers such as saccharides (for example, lactose, sucrose, mannitol or sorbitol), cellulose preparations, calcium phosphates (for example, tricalcium phosphate or calcium hydrogen phosphate), as well as binders such as starch paste (using, for example, maize starch, wheat starch, rice starch, or potato starch), gelatin, tragacanth, methyl cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvinyl pyrrolidone.
• saccharides for example, lactose, sucrose, mannitol or sorbitol
• cellulose preparations for example, calcium phosphates (for example, tricalcium phosphate or calcium hydrogen phosphate)
• binders such as starch paste (using, for example, maize starch, wheat starch, rice starch, or potato starch), gelatin, tragacanth, methyl cellulose, hydroxypropylmethyl
• one or more disintegrating agents can be added, such as the above-mentioned starches and also carboxymethyl-starch, cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof, such as sodium alginate.
• Auxiliaries are typically flow-regulating agents and lubricants such as, for example, silica, talc, stearic acid or salts thereof (e.g., magnesium stearate or calcium stearate), and polyethylene glycol.
• Dragee cores are provided with suitable coatings that are resistant to gastric juices.
• concentrated saccharide solutions can be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, polyethylene glycol and/or titanium dioxide, lacquer solutions and suitable organic solvents or solvent mixtures.
• suitable cellulose preparations such as acetylcellulose phthalate or hydroxypropylmethyl-cellulose phthalate can be used.
• Dye stuffs or pigments can be added to the tablets or dragee coatings, for example, for identification or in order to characterize combinations of active compound doses.
• Examples of other pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin, or soft, sealed capsules made of gelatin and a plasticizer such as glycerol or sorbitol.
• the push-fit capsules can contain a compound in the form of granules, which can be mixed with fillers such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers, or in the form of extruded multiparticulates.
• the active compounds are preferably dissolved or suspended in suitable liquids, such as fatty oils or liquid paraffin.
• stabilizers can be added.
• Possible pharmaceutical preparations for rectal administration include, for example, suppositories, which consist of a combination of one or more active compounds with a suppository base.
• Suitable suppository bases include natural and synthetic triglycerides, and paraffin hydrocarbons, among others. It is also possible to use gelatin rectal capsules consisting of a combination of active compound with a base material such as, for example, a liquid triglyceride, polyethylene glycol, or paraffin hydrocarbon.
• Suitable formulations for parenteral administration include aqueous solutions of the active compound in a water-soluble form such as, for example, a water-soluble salt, alkaline solution, or acidic solution.
• a suspension of the active compound can be prepared as an oily suspension.
• Suitable lipophilic solvents or vehicles for such as suspension may include fatty oils (for example, sesame oil), synthetic fatty acid esters (for example, ethyl oleate), triglycerides, or a polyethylene glycol such as polyethylene glycol-400 (PEG-400).
• An aqueous suspension may contain one or more substances to increase the viscosity of the suspension, including, for example, sodium carboxymethyl cellulose, sorbitol, and/or dextran.
• the suspension may optionally contain stabilizers.
• the Compound of the Disclosure and the Second Therapeutic Agent are administered in combination to a subject as part of a single pharmaceutical composition.
• the Compound of the Disclosure and the Second Therapeutic Agent are administered in combination to a subject separately, e.g., as two or more separate pharmaceutical compositions.
• the Second Therapeutic Agent may comprise one of a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a Bcl-2 inhibitor, a pleiotropic pathway modulator, a XPO1 inhibitor, a histone deacetylase inhibitor, or an EZH2 inhibitor.
• two separate pharmaceutical compositions – one comprising the Compound of the Disclosure and one comprising the Second Therapeutic Agent — are administered to a subject.
• the Second Therapeutic Agent may comprise a combination of two of a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a Bcl-2 inhibitor, a pleiotropic pathway modulator, a XPO1 inhibitor, a histone deacetylase inhibitor, or an EZH2 inhibitor.
• three separate pharmaceutical compositions – one comprising the Compound of the Disclosure, one comprising the first Second Therapeutic Agent, and one comprising the second Second Therapeutic Agent – are administered to a subject.
• the Second Therapeutic Agent comprises a combination of three or more of a glucocorticoid receptor agonist, an immunomodulatory drug, a proteasome inhibitor, a Bcl-2 inhibitor, a pleiotropic pathway modulator, a XPO1 inhibitor, a histone deacetylase inhibitor, or an EZH2 inhibitor.
• the separate pharmaceutical compositions can be administered to the subject, for example, at different periodicities, at different durations, or by different administration routes.
• a Compound of the Disclosure is administered to the patient prior to the Second Therapeutic Agent, e.g., 0.5, 1, 2, 3, 4, 5, 10, 12, or 18 hours, 1, 2, 3, 4, 5, or 6 days, or 1, 2, 3, or 4 weeks prior to the administration of the Second Therapeutic Agent.
• a Compound of the Disclosure is administered after the Second Therapeutic Agent, e.g., 0.5, 1, 2, 3, 4, 5, 10, 12, or 18 hours, 1, 2, 3, 4, 5, or 6 days, or 1, 2, 3, or 4 weeks after the administration of the Second Therapeutic Agent.
• a Compound of the Disclosure and the Second Therapeutic Agent are administered concurrently.
• kits which comprise a Compound of the Disclosure (or a composition comprising a Compound of the Disclosure) packaged in a manner that facilitates their use to practice methods of the present disclosure.
• the kit includes a Compound of the Disclosure (or a composition comprising a Compound of the Disclosure) packaged in a container, such as a sealed bottle or vessel, with a label affixed to the container or included in the kit that describes use of the compound or composition to practice the method of the disclosure.
• the compound or composition is packaged in a unit dosage form.
• the kit further can include a device suitable for administering the composition according to the intended route of administration.
• the kit further can include a Second Therapeutic Agent.
• the kit comprises a Compound of the Disclosure and a Second Therapeutic Agent as separate pharmaceutical compositions. IV.
• present disclosure provides methods of treating a subject having cancer, e.g., multiple myeloma, comprising (a) determining whether a biomarker is present or absent in a biological sample taken from the subject; and (b) administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent to the subject if the biomarker is present in the biological sample.
• a biomarker e.g., multiple myeloma
• administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent to the subject if the biomarker is present in the biological sample.
• Biomarkers include, but are not limited to, chromosomal translocations in a cancer, e.g., mulitple myeloma, cell and WHSC1/NSD2/MMSET expression.
• the measurable aspect of the biomarker is its expression status. In one embodiment, the measurable aspect of the biomarker is its mutation status.
• the biomarker is WHSC1/NSD2/MMSET expression which is differentially present in a subject of one phenotypic status, e.g., a subject having a hematological cancer, as compared with another phenotypic status, e.g., a normal undiseased subject or a patient having cancer without overexpression WHSC1/NSD2/MMSET.
• the biomarker is overexpression of WHSC1/NSD2/MMSET.
• Biomarker standards can be predetermined, determined concurrently, or determined after a biological sample is obtained from the subject.
• Biomarker standards for use with the methods described herein can, for example, include data from samples from subjects without cancer; data from samples from subjects with cancer, e.g., breast cancer, that is not metastatic; and data from samples from subjects with cancer, e.g., breast cancer, that metastatic. Comparisons can be made to establish predetermined threshold biomarker standards for different classes of subjects, e.g., diseased vs. non- diseased subjects. The standards can be run in the same assay or can be known standards from a previous assay. [0639] A biomarker is differentially present between different phenotypic status groups if the mean or median expression or mutation levels of the biomarker is calculated to be different, i.e., higher or lower, between the groups.
• biomarkers provide an indication that a subject, e.g., a cancer patient, belongs to one phenotypic status or another.
• the determination of the expression level or mutation status of a biomarker in a patient can be performed using any of the many methods known in the art. Any method known in the art for quantitating specific proteins and/or detecting WHSC1/NSD2/MMSET expression and/or chromosomal translocations, or the expression or mutation levels of any other biomarker in a patient or a biological sample may be used in the methods of the disclosure.
• RNA expression examples include, but are not limited to, PCR (polymerase chain reaction), or RT-PCR, flow cytometry, Northern blot, Western blot, ELISA (enzyme linked immunosorbent assay), RIA (radioimmunoassay), gene chip analysis of RNA expression, immunohistochemistry or immunofluorescence.
• PCR polymerase chain reaction
• RT-PCR flow cytometry
• Northern blot Northern blot
• Western blot Western blot
• ELISA enzyme linked immunosorbent assay
• RIA radioimmunoassay
• a biological sample is obtained from the patient and the biological sample is assayed for determination of a biomarker expression or mutation status.
• the present disclosure provides a method of treating a subject having cancer, e.g., multiple myeloma, the method comprising: (a) determining whether a chromosomal translocation is present or absent in a biological sample taken from the subject; and (b) administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Theraputic Agent to the subject if a chromosomal translocation is present in the biological sample.
• the present disclosure provides a method of treating a subject having cancer, e.g., multiple myeloma, the method comprising administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapetuic Agent to the subject having a chromosomal translocation.
• the present disclosure provides a method, comprising administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent, to a subject in need thereof, wherein: (a) the subject has multiple myeloma; and (b) the multiple myeloma is characterized as having a chromosomal translocation.
• the chromosomal translocation is a t(4;14) translocation.
• the present disclosure provides a method of treating a subject having multiple myeloma, the method comprising: (a) determining whether an overexpression of WHSC1/NSD2/MMSET is present or absent in a biological sample taken from the subject; and (b) administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent, to the subject if an overexpression of WHSC1/NSD2/MMSET is present in the biological sample.
• the present disclosure provides a method of treating a subject having multiple myeloma, the method comprising administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent, to the subject if an overexpression of WHSC1/NSD2/MMSET is present in subject.
• the present disclosure provides a method, comprising administering a therapeutically effective amount of a Compound of the Disclosure and, optionally, a Second Therapeutic Agent, to a subject in need thereof, wherein: (a) the subject has multiple myeloma; and (b) the multiple myeloma is characterized as having an overexpression of WHSC1/NSD2/MMSET. V.
• halo as used herein by itself or as part of another group refers to -Cl, -F, -Br, or -I.
• nitro as used herein by itself or as part of another group refers to -NO 2 .
• cyano as used herein by itself or as part of another group refers to -CN.
• hydroxy as herein used by itself or as part of another group refers to -OH.
• alkyl refers to a straight- or branched-chain aliphatic hydrocarbon containing one to twelve carbon atoms, i.e., a C 1 -C 12 alkyl, or the number of carbon atoms designated, e.g., a C 1 alkyl such as methyl, a C 2 alkyl such as ethyl, etc.
• the alkyl is a C 1 -C 10 alkyl.
• the alkyl is a C1-C6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C 1 -C 3 alkyl, i.e., methyl, ethyl, propyl, or isopropyl.
• Non-limiting exemplary C 1 -C 12 alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tert-butyl, iso-butyl, 3-pentyl, hexyl, heptyl, octyl, nonyl, and decyl.
• the optionally substituted alkyl is substituted with two substituents. In another embodiment, the optionally substituted alkyl is substituted with one substituent. In another embodiment, the optionally substituted alkyl is an optionally substituted C1-C6 alkyl. In another embodiment, the optionally substituted alkyl is an optionally substituted C 1 -C 4 alkyl. In one embodiment, the optionally substituted alkyl is an optionally substituted is a C1 or C2 alkyl.
• alkenyl refers to an alkyl group containing one, two, or three carbon-to-carbon double bonds.
• the alkenyl group is a C 2 -C 6 alkenyl group.
• the alkenyl group is a C2-C4 alkenyl group.
• the alkenyl group has one carbon-to-carbon double bond.
• Non-limiting exemplary alkenyl groups include ethenyl, propenyl, isopropenyl, butenyl, sec-butenyl, pentenyl, and hexenyl.
• alkenyl as used herein by itself or as part of another refers to an alkenyl group that is either unsubstituted or substituted with one, two or three substituents, wherein each substituent is independently halo, nitro, cyano, hydroxy, amino (e.g., alkylamino, dialkylamino), haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfonyl, arylsulfonyl, ureido, guanidino, carboxy, carboxyalkyl, optionally substituted cycloalkyl, alkenyl, alkynyl, optionally substituted aryl, optionally substituted heteroaryl, or optionally substituted heterocycl
• alkynyl as used herein by itself or as part of another group refers to an alkyl group containing one, two, or three carbon-to-carbon triple bonds. In one embodiment, the alkynyl has one carbon-to-carbon triple bond. In another embodiment, the alkynyl is a C 1 -C 6 alkynyl. In another embodiment, the alkynyl is a C 2 -C 4 alkynyl. In another embodiment, the alkynyl has one carbon-to-carbon triple bond.
• Non-limiting exemplary alkynyl groups include ethynyl, propynyl, butynyl, 2-butynyl, pentynyl, and hexynyl groups.
• optionally substituted alkynyl refers to an alkynyl group that is either unsubstituted or substituted with one, two or three substituents, wherein each substituent is independently halo, nitro, cyano, hydroxy, amino (e.g., alkylamino, dialkylamino), haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfonyl, arylsulfonyl, ureido,
• Non-limiting exemplary optionally substituted alkynyl groups include -CH ⁇ CHPh.
• haloalkyl as used herein by itself or as part of another group refers to an alkyl group substituted by one or more fluorine, chlorine, bromine, and/or iodine atoms.
• the alkyl is substituted by one, two, or three fluorine and/or chlorine atoms.
• the alkyl is substituted by one, two, or three fluorine atoms.
• the alkyl is a C1-C6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl group is a C 1 or C 2 alkyl.
• Non-limiting exemplary haloalkyl groups include fluoromethyl, difluoromethyl, trifluoromethyl, pentafluoroethyl, 1,1-difluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, 3,3,3-trifluoropropyl, 4,4,4-trifluorobutyl, and trichloromethyl groups.
• hydroxyalkyl or "(hydroxy)alkyl” as used herein by themselves or as part of another group refer to an alkyl group substituted with one, two, or three hydroxy groups.
• the alkyl is a C 1 -C 6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C 1 or C 2 alkyl.
• the hydroxyalkyl is a monohydroxyalkyl group, i.e., substituted with one hydroxy group. In another embodiment, the hydroxyalkyl group is a dihydroxyalkyl group, i.e., substituted with two hydroxy groups.
• Non-limiting exemplary (hydroxyl)alkyl groups include hydroxymethyl, hydroxyethyl, hydroxypropyl and hydroxybutyl groups, such as 1-hydroxyethyl, 2-hydroxyethyl, 1,2-dihydroxyethyl, 2-hydroxypropyl, 3-hydroxypropyl, 3-hydroxybutyl, 4-hydroxybutyl, 2-hydroxy-1- methylpropyl, and 1,3-dihydroxyprop-2-yl.
• alkoxy as used herein by itself or as part of another group refers to an alkyl group attached to a terminal oxygen atom. In one embodiment, the alkyl is a C 1 -C 6 alkyl.
• the alkyl is a C 1 -C 4 alkyl group.
• Non-limiting exemplary alkoxy groups include methoxy, ethoxy, and tert-butoxy.
• haloalkoxy as used herein by itself or as part of another group refers to a haloalkyl group attached to a terminal oxygen atom.
• the haloalkyl is a C1-C6 alkyl.
• the haloalkyl group is a C 1 -C 4 haloalkyl group.
• Non-limiting exemplary haloalkoxy groups include fluoromethoxy, difluoromethoxy, trifluoromethoxy, and 2,2,2-trifluoroethoxy.
• alkylthio refers to an alkyl group attached to a terminal sulfur atom.
• the alkyl group is a C 1 -C 4 alkyl group.
• Non-limiting exemplary alkylthio groups include -SCH3, and -SCH2CH3.
• alkoxyalkyl or “(alkoxy)alkyl” as used herein by themselves or as part of another group refers to an alkyl group substituted with one alkoxy group.
• the alkoxy is a C1-C6 alkoxy.
• the alkoxy is a C 1 -C 4 alkoxy.
• the alkyl is a C 1 -C 6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl.
• Non-limiting exemplary alkoxyalkyl groups include methoxymethyl, methoxyethyl, methoxypropyl, methoxybutyl, ethoxymethyl, ethoxyethyl, ethoxypropyl, ethoxybutyl, propoxymethyl, iso-propoxymethyl, propoxyethyl, propoxypropyl, butoxymethyl, tert-butoxymethyl, isobutoxymethyl, sec- butoxymethyl, and pentyloxymethyl.
• heteroalkyl refers to a stable straight or branched chain hydrocarbon radical containing 1 to 10 carbon atoms and at least two heteroatoms, which can be the same or different, selected from O, N, or S, wherein the sulfur atom(s) can optionally be oxidized.
• the heteroatoms can be placed at any interior position of the heteroalkyl group or at a position at which the heteroalkyl group is attached to the remainder of the molecule.
• the heteroalkyl contains two oxygen atoms.
• the heteroalkyl contains one oxygen and one nitrogen atom.
• the heteroalkyl contains two nitrogen atoms.
• Non-limiting exemplary heteroalkyl groups include -OCH 2 CH 2 NH 2 , -NHCH2CH2OCH3, and -OCH2CH2OCH3.
• cycloalkyl as used herein by itself or as part of another group refers to saturated and partially unsaturated, e.g., containing one or two double bonds, monocyclic, bicyclic, or tricyclic aliphatic hydrocarbons containing three to twelve carbon atoms, i.e., a C3-12 cycloalkyl, or the number of carbons designated, e.g., a C3 cycloalkyl such a cyclopropyl, a C 4 cycloalkyl such as cyclobutyl, etc.
• the cycloalkyl is bicyclic, i.e., it has two rings. In another embodiment, the cycloalkyl is monocyclic, i.e., it has one ring. In another embodiment, the cycloalkyl is a C3-8 cycloalkyl. In another embodiment, the cycloalkyl is a C 3-6 cycloalkyl, i.e., cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl. In another embodiment, the cycloalkyl is a C 5 cycloalkyl, i.e., cyclopentyl.
• the cycloalkyl is a C6 cycloalkyl, i.e., cyclohexyl.
• Non-limiting exemplary C3-12 cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, norbornyl, decalin, adamantyl, cyclohexenyl, and spiro[3.3]heptane.
• cycloalkyl as used herein by itself or as part of another group refers to a cycloalkyl group is either unsubstituted or substituted with one, two, or three substituents, wherein each substituent is independently halo, nitro, cyano, hydroxy, amino (e.g., -NH2, alkylamino, dialkylamino, aralkylamino, hydroxyalkylamino, or (heterocyclo)alkylamino), heteroalkyl, haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyl, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfonyl, arylsulfonyl, ureido, guanidino, carboxy,
• Non-limiting exemplary optionally substituted cycloalkyl groups include 3-(4-acetylpiperazin-1- yl)cyclohexyl, 3-(3-(N-methylacetamido)pyrrolidin-1-yl)cyclohexyl, 3- morpholinocyclohexyl, and 3-(pyrimidin-5-yl)cyclohexyl.
• the optionally substituted cycloalkyl is either unsubstituted or substituted with one, two, or three substituents, wherein each substituent is independently halo, nitro, cyano, hydroxy, amino (e.g., -NH2, alkylamino, dialkylamino, aralkylamino, hydroxyalkylamino, or (heterocyclo)alkylamino), heteroalkyl, haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyl, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfonyl, arylsulfonyl, ureido, guanidino, carboxy, carboxyalkyl, optionally substituted alkyl, optionally substituted cycloalkyl, alken
• heterocyclo refers to saturated and partially unsaturated, e.g., containing one or two double bonds, monocyclic, bicyclic, or tricyclic groups containing three to fourteen ring members, i.e., a 3- to 14-membered heterocyclo, comprising one, two, three, or four heteroatoms.
• Each heteroatom is independently oxygen, sulfur, or nitrogen.
• heterocyclo also includes groups having fused optionally substituted aryl or optionally substituted heteroaryl groups such as indoline, indolin-2-one, 2,3-dihydro-1H-pyrrolo[2,3-c]pyridine, 2,3,4,5-tetrahydro-1H-benzo[d]azepine, or 1,3,4,5-tetrahydro-2H-benzo[d]azepin-2-one.
• the heterocyclo group is a 8- to12-membered cyclic group containing two rings and one or two nitrogen atoms. The heterocyclo can be linked to the rest of the molecule through any available carbon or nitrogen atom.
• Non-limiting exemplary heterocyclo groups include: [0689]
• the term "optionally substituted heterocyclo" as used herein by itself or part of another group refers to a heterocyclo group that is either unsubstituted or substituted with one to four substituents, wherein each substituent is independently halo, nitro, cyano, hydroxy, amino, (e.g., -NH 2 , alkylamino, dialkylamino, aralkylamino, hydroxyalkylamino, or (heterocyclo)alkylamino), heteroalkyl, haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyl, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfonyl, arylsulfonyl, ureido,
• the optionally substituted heterocyclo is either unsubstituted or substituted with one to four substituents, wherein each substituent is independently halo, nitro, cyano, hydroxy, amino, (e.g., -NH 2 , alkylamino, dialkylamino, aralkylamino, hydroxyalkylamino, or (heterocyclo)alkylamino), heteroalkyl, haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyl, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfonyl, arylsulfonyl, ureido, guanidino, carboxy, carboxyalkyl, optionally substituted alkyl, optionally substituted cycloalkyl, alkenyl,
• Non-limiting exemplary optionally substituted heterocyclo groups include: [0691]
• the term "aryl” as used herein by itself or as part of another group refers to an aromatic ring system having six to fourteen carbon atoms, i.e., C 6 -C 14 aryl.
• Non-limiting exemplary aryl groups include phenyl (abbreviated as "Ph"), naphthyl, phenanthryl, anthracyl, indenyl, azulenyl, biphenyl, biphenylenyl, and fluorenyl groups.
• the aryl group is phenyl or naphthyl.
• the aryl group is phenyl.
• aryl that is either unsubstituted or substituted with one to five substituents, wherein the substituents are each independently halo, nitro, cyano, hydroxy, amino, (e.g., -NH2, alkylamino, dialkylamino, aralkylamino, hydroxyalkylamino, or (heterocyclo)alkylamino), heteroalkyl, haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyl, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfonyl, arylsulfonyl, ureido, guanidino, carboxy, carboxyalkyl, optionally
• the optionally substituted aryl is an optionally substituted phenyl. In another embodiment, the optionally substituted phenyl has four substituents. In another embodiment, the optionally substituted phenyl has three substituents. In another embodiment, the optionally substituted phenyl has two substituents. In another embodiment, the optionally substituted phenyl has one substituent.
• Non-limiting exemplary optionally substituted aryl groups include 2-methylphenyl, 2-methoxyphenyl, 2-fluorophenyl, 2-chlorophenyl, 2-bromophenyl, 3-methylphenyl, 3-methoxyphenyl, 3- fluorophenyl, 3-chlorophenyl, 4-methylphenyl, 4-ethylphenyl, 4-methoxyphenyl, 4-fluorophenyl, 4-chlorophenyl, 2,6-di-fluorophenyl, 2,6-di-chlorophenyl, 2-methyl, 3-methoxyphenyl, 2-ethyl, 3-methoxyphenyl, 3,4-di-methoxyphenyl, 3,5-di-fluorophenyl 3,5-di-methylphenyl, 3,5-dimethoxy, 4-methylphenyl, 2-fluoro-3-chlorophenyl, 3-chloro- 4-fluorophenyl, and 2-phenylpropan-2-
• optionally substituted aryl includes aryl groups having fused optionally substituted cycloalkyl groups and fused optionally substituted heterocyclo groups.
• Non-limiting examples include: 2,3-dihydro- 1H-inden-1-yl, 1,2,3,4-tetrahydronaphthalen-1-yl, 1,3,4,5-tetrahydro-2H-benzo[c]azepin- 2-yl, 1,2,3,4-tetrahydroisoquinolin-1-yl, and 2-oxo-2,3,4,5-tetrahydro-1H- benzo[d]azepin-1-yl.
• heteroaryl refers to monocyclic and bicyclic aromatic ring systems having five to 14 fourteen ring members, i.e., a 5- to 14-membered heteroaryl, comprising one, two, three, or four heteroatoms.
• Each heteroatom is independently oxygen, sulfur, or nitrogen.
• the heteroaryl has three heteroatoms.
• the heteroaryl has two heteroatoms.
• the heteroaryl has one heteroatom.
• the heteroaryl is a 5- to 10-membered heteroaryl.
• the heteroaryl has 5 ring atoms, e.g., thienyl, a 5-membered heteroaryl having four carbon atoms and one sulfur atom.
• the heteroaryl has 6 ring atoms, e.g., pyridyl, a 6-membered heteroaryl having five carbon atoms and one nitrogen atom.
• Non-limiting exemplary heteroaryl groups include thienyl, benzo[b]thienyl, naphtho[2,3-b]thienyl, thianthrenyl, furyl, benzofuryl, pyranyl, isobenzofuranyl, benzooxazonyl, chromenyl, xanthenyl, 2H-pyrrolyl, pyrrolyl, imidazolyl, pyrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, isoindolyl, 3H-indolyl, indolyl, indazolyl, purinyl, isoquinolyl, quinolyl, phthalazinyl, naphthyridinyl, cinnolinyl, quinazolinyl, pteridinyl, 4aH-carbazolyl, carbazolyl, ⁇ -carboliny
• the heteroaryl is chosen from thienyl (e.g., thien-2-yl and thien-3-yl), furyl (e.g., 2-furyl and 3-furyl), pyrrolyl (e.g., 1H-pyrrol-2-yl and 1H-pyrrol-3-yl), imidazolyl (e.g., 2H-imidazol-2-yl and 2H- imidazol-4-yl), pyrazolyl (e.g., 1H-pyrazol-3-yl, 1H-pyrazol-4-yl, and 1H-pyrazol-5-yl), pyridyl (e.g., pyridin-2-yl, pyridin-3-yl, and pyridin-4-yl), pyrimidinyl (e.g., pyrimidin-2- yl, pyrimidin-4-yl, and pyrimidin-5-yl), thiazolyl (e.g.
• heteroaryl also includes N-oxides.
• a non-limiting exemplary N-oxide is pyridyl N-oxide.
• the term "optionally substituted heteroaryl" as used herein by itself or as part of another group refers to a heteroaryl that is either unsubstituted or substituted with one to four substituents, wherein the substituents are independently halo, nitro, cyano, hydroxy, amino, (e.g., -NH2, alkylamino, dialkylamino, aralkylamino, hydroxyalkylamino, or (heterocyclo)alkylamino), heteroalkyl, haloalkyl, hydroxyalkyl, alkoxy, haloalkoxy, aryloxy, aralkyl, aralkyloxy, alkylthio, carboxamido, sulfonamido, alkylcarbonyl, arylcarbonyl, alkylsulfon
• the optionally substituted heteroaryl has two substituents. In another embodiment, the optionally substituted heteroaryl has one substituent. Any available carbon or nitrogen atom can be substituted.
• aryloxy as used herein by itself or as part of another group refers to an optionally substituted aryl attached to a terminal oxygen atom. A non-limiting exemplary aryloxy group is PhO-.
• heteroaryloxy as used herein by itself or as part of another group refers to an optionally substituted heteroaryl attached to a terminal oxygen atom.
• a non-limiting exemplary aryloxy group is pyridyl-O-.
• aralkyloxy refers to an aralkyl attached to a terminal oxygen atom.
• a non-limiting exemplary aralkyloxy group is PhCH2O-.
• (cycloalkyl)oxy refers to a cycloalkyl group attached to a terminal oxygen atom.
• a non-limiting exemplary cycloalkyloxy group is: [0701]
• the term "(heterocyclo)oxy” as used herein by itself or as part of another group refers to a heterocyclo group attached to a terminal oxygen atom.
• a non-limiting exemplary (heterocyclo)oxy group is: [0702]
• the term "(cyano)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with one, two, or three cyano groups. In one embodiment, the alkyl is substituted with one cyano group. In another embodiment, the alkyl is a C1-C6 alkyl In another embodiment, the alkyl is a C 1 -C 4 alkyl.
• Non-limiting exemplary (cyano)alkyl groups include -CH 2 CH 2 CN and -CH 2 CH 2 CH 2 CN.
• (cycloalkyl)alkyl refers to an alkyl substituted with one optionally substituted cycloalkyl group.
• the cycloalkyl group is an optionally substituted C 3 -C 6 cycloalkyl.
• the alkyl is a C 1 -C 6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• Non-limiting exemplary (cycloalkyl)alkyl groups include: [0704]
• the term "sulfonamido" as used herein by itself or as part of another group refers to a radical of the formula -SO 2 NR 50a R 50b , wherein R 50a and R 50b are each independently hydrogen, alkyl, optionally substituted cycloalkyl, optionally substituted heterocyclo, optionally substituted aryl, or optionally substituted heteroaryl; or R 50a and R 50b taken together with the nitrogen to which they are attached form a 3- to 8-membered optionally substituted heterocyclo group.
• Non-limiting exemplary sulfonamido groups include -SO2NH2, -SO2N(H)CH3, and -SO2N(H)Ph.
• the alkyl is a C 1 -C 4 alkyl.
• a non-limiting exemplary alkylcarbonyl group is -COCH3.
• a non-limiting exemplary arylcarbonyl group is -COPh.
• alkylsulfonyl as used herein by itself or as part of another group refers to a sulfonyl group, i.e., -SO 2 -, substituted by an alkyl group.
• a non-limiting exemplary alkylsulfonyl group is -SO2CH3.
• arylsulfonyl refers to a sulfonyl group, i.e., -SO 2 -, substituted by an optionally substituted aryl group.
• a non-limiting exemplary arylsulfonyl group is -SO 2 Ph.
• mercaptoalkyl refers to an alkyl substituted by a –SH group.
• the term "(heterocyclo)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with one, two, or three optionally substituted heterocyclo groups. In one embodiment, the alkyl is substituted with one optionally substituted 5- to 8-membered heterocyclo group. In another embodiment, alkyl is a C1-C6 alkyl. In another embodiment, alkyl is a C 1 -C 4 alkyl. The heterocyclo group can be linked to the alkyl group through a carbon or nitrogen atom.
• Non-limiting exemplary (heterocyclo)alkyl groups include:
• R 54a is hydrogen or alkyl
• R 54b is hydrogen, alkyl, optionally substituted cycloalkyl, optionally substituted heterocyclo, optionally substituted aryl, or optionally substituted heteroaryl.
• (heteroaryl)alkyl as used herein by itself or as part of another group refers to an alkyl substituted with one or two optionally substituted heteroaryl groups.
• the alkyl group is substituted with one optionally substituted 5- to 14-membered heteroaryl group. In another embodiment, the alkyl group is substituted with two optionally substituted 5- to 14-membered heteroaryl groups. In another embodiment, the alkyl group is substituted with one optionally substituted 5- to 9-membered heteroaryl group. In another embodiment, the alkyl group is substituted with two optionally substituted 5- to 9-membered heteroaryl groups. In another embodiment, the alkyl group is substituted with one optionally substituted 5- or 6-membered heteroaryl group. In another embodiment, the alkyl group is substituted with two optionally substituted 5- or 6-membered heteroaryl groups.
• the alkyl group is a C1-C6 alkyl. In another embodiment, the alkyl group is a C 1 -C 4 alkyl. In another embodiment, the alkyl group is a C 1 or C 2 alkyl.
• Non-limiting exemplary (heteroaryl)alkyl groups include: [0716] The term "(heteroaryl)(aryl)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted heteroaryl group and one optionally substituted aryl group. In one embodiment, the heteroaryl is an optionally substituted 5- to 9-membered heteroaryl group.
• the heteroaryl is an optionally substituted 5- or 6-membered heteroaryl group.
• the aryl is an optionally substituted phenyl group or optionally substituted naphthyl group.
• the alkyl is a C 1 -C 6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C 1 or C 2 alkyl.
• Non-limiting exemplary (heteroaryl)(aryl)alkyl groups include: [0717]
• the term "(heteroaryl)(heterocyclo)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted heteroaryl group and one optionally substituted heterocyclo group.
• the heteroaryl is an optionally substituted 5- to 9-membered heteroaryl group.
• the heteroaryl is an optionally substituted 5- or 6-membered heteroaryl group.
• the heterocyclo is an optionally substituted 5- to 8-membered heterocyclo.
• the heterocyclo is an optionally substituted 5- or 6-membered heterocyclo.
• the alkyl is a C1-C6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C 1 or C 2 alkyl.
• a non-limiting exemplary (heteroaryl)(heterocyclo)alkyl group is: [0718]
• the term "(heteroaryl)(carboxamido)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted heteroaryl group and one carboxamido group.
• the heteroaryl is an optionally substituted 5- to 9-membered heteroaryl group.
• the heteroaryl is an optionally substituted 5- or 6-membered heteroaryl group.
• the alkyl is a C 1 -C 6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1-C3 alkyl.
• Non-limiting exemplary (heteroaryl)(carboxamido)alkyl groups include: [0719]
• Non-limiting exemplary carboxamido groups include: morpholine-4-carbonyl, N,N- dimethylaminocarbonyl, N-(1-methylpiperidin-4-yl)aminocarbonyl, 4-methylpiperazine- 1-carbonyl, N-(3-aminocyclopentyl)aminocarbonyl, N-(pyridin-3-yl)aminocarbonyl, and N-(tetrahydrofuran-3-yl)aminocarbonyl.
• heteroaryl (cycloalkyl)alkyl
• cycloalkyl is an optionally substituted C 3 -C 6 cycloalkyl.
• alkyl is a C1-C6 alkyl.
• alkyl is a C1- C4 alkyl.
• the alkyl is a C1-C3 alkyl.
• a non-limiting exemplary (heteroaryl)(C 3 -C 6 cycloalkyl) alkyl group is: [0721]
• the term "(aryl)(alkoxycarbonyl)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted aryl group and one alkoxycarbonyl group.
• the aryl is an optionally substituted phenyl group or optionally substituted naphthyl group.
• the aryl is an optionally substituted phenyl group.
• the alkyl is a C 1 -C 6 alkyl.
• the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (aryl)(alkoxycarbonyl)alkyl group is: [0722]
• Non-limiting exemplary alkoxycarbonyl groups include -CO2- Me and -CO2Et.
• the term "(heteroaryl)(amino)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted heteroaryl group and one amino group.
• the heteroaryl is an optionally substituted 5- to 9-membered heteroaryl group.
• the heteroaryl is an optionally substituted 5- or 6-membered heteroaryl group.
• the alkyl is a C1-C6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (heteroaryl)(amino)alkyl group is: [0724]
• the term "(cycloalkyl)(alkoxycarbonyl)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted cycloalkyl group and one alkoxycarbonyl group.
• the cycloalkyl is an optionally substituted C3-C6 cycloalkyl.
• the alkyl is a C1-C6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C 1 or C 2 alkyl.
• a non-limiting exemplary (cycloalkyl)(alkoxycarbonyl)alkyl group is: [0725]
• the term "(heteroaryl)(alkoxycarbonyl)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted heteroaryl group and one alkoxycarbonyl group.
• the heteroaryl is an optionally substituted 5- to 9-membered heteroaryl group.
• the heteroaryl is an optionally substituted 5- or 6-membered heteroaryl group.
• the alkyl is a C 1 -C 6 alkyl.
• the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C1 or C2 alkyl.
• Non-limiting exemplary (heteroaryl)(alkoxycarbonyl)alkyl groups include: [0726]
• the term "(heterocyclo)(cycloalkyl)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted heterocyclo group and one optionally substituted cycloalkyl group.
• the heterocyclo is an optionally substituted 5- to 8-membered heterocyclo. In another embodiment, the heterocyclo is an optionally substituted 5- or 6-membered heterocyclo.
• the cycloalkyl is an optionally substituted C3-C6 cycloalkyl.
• the alkyl is a C 1 -C 6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (heterocyclo)(cycloalkyl)alkyl group is: [0727]
• the term "(aryl)(cycloalkyl)alkyl" as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted aryl group and one optionally substituted cycloalkyl group.
• the aryl is an optionally substituted phenyl group or optionally substituted naphthyl group. In another embodiment, the aryl is an optionally substituted phenyl group. In one embodiment, the cycloalkyl is an optionally substituted C3-C6 cycloalkyl. In one embodiment, the alkyl is a C 1 -C 6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (aryl)(cycloalkyl)alkyl group is: [0728]
• the terms "aralkyl” or "(aryl)alkyl” as used herein by themselves or as part of another group refers to an alkyl substituted with one, two, or three optionally substituted aryl groups.
• the alkyl is substituted with one optionally substituted aryl group.
• the alkyl is substituted with two optionally substituted aryl groups.
• the aryl is an optionally substituted phenyl or optionally substituted naphthyl.
• the aryl is an optionally substituted phenyl.
• the alkyl is a C 1 -C 6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C 1 or C 2 alkyl.
• Non-limiting exemplary (aryl)alkyl groups include benzyl, phenethyl, -CHPh2, and -CH(4-F-Ph)2.
• (aryl)(hydroxy)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with one optionally substituted aryl group and one hydroxyl group.
• the aryl is an optionally substituted phenyl group or optionally substituted naphthyl group. In another embodiment, the aryl is an optionally substituted phenyl group.
• the alkyl is a C 1 -C 6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl. In another embodiment, the alkyl is a C1 or C2 alkyl.
• Non-limiting exemplary (aryl)(hydroxy)alkyl groups include: [0730]
• the term "(cycloalkyl)(hydroxy)alkyl” as used herein by itself or as part of another group refers to an alkyl group substituted with one optionally substituted cycloalkyl group and one hydroxyl group.
• the cycloalkyl group is an optionally substituted C3-C6 cycloalkyl group.
• the alkyl is a C1-C6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (cycloalkyl)(hydroxy)alkyl group is: [0731]
• the term "(alkoxycarbonyl)alkyl” as used herein by itself or as part of another group refers to an alkyl substituted with one or two alkoxycarbonyl groups.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (alkoxycarbonyl)alkyl groups is: [0732]
• the term "(aryl)(haloalkyl)alkyl” as used herein by itself or as part of another group refers to an alkyl substituted with one optionally substituted aryl group and one haloalkyl group.
• the aryl is an optionally substituted group or optionally substituted naphthyl.
• the aryl is an optionally substituted phenyl.
• the haloalkyl is a C 1 -C 4 haloalkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C 1 or C 2 alkyl.
• a non-limiting exemplary (aryl)(haloalkyl)alkyl groups is: [0733]
• the term "(cycloalkyl)(haloalkyl)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with one optionally substituted cycloalkyl group and one haloalkyl group.
• the cycloalkyl is an optionally substituted C 3 -C 6 cycloalkyl.
• the haloalkyl is a C 1 -C 4 haloalkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (cycloalkyl)( haloalkyl) alkyl groups is: [0734]
• the term "(hydroxy)(haloalkyl)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with one hydroxy group and one haloalkyl group.
• the haloalkyl is a C 1 -C 4 haloalkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C 1 or C 2 alkyl.
• a non-limiting exemplary (hydroxy)( haloalkyl)alkyl groups is: .
• the term "(alkoxycarbonyl)(haloalkyl)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with one alkoxycarbonyl group and one haloalkyl group.
• the haloalkyl is a C 1 -C 4 haloalkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• a non-limiting exemplary (alkoxycarbonyl)(haloalkyl)alkyl groups is: [0736]
• the term "(carboxamido)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with a carboxamido group.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C1 or C2 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• the alkyl is a C 1 or C 2 alkyl.
• a non-limiting exemplary (carboxy)alkyl group is -CH 2 CO 2 H.
• (amino)(hydroxy)alkyl refers to an alkyl substituted with one hydroxy group and one amino group.
• the alkyl is a C 1 -C 4 alkyl.
• a non-limiting exemplary "(amino)(hydroxy)alkyl group is:
• the term "(amino)(aryl)alkyl” as used herein by itself or as part of another group refers to an alkyl group substituted with one amino group and one optionally substituted aryl group.
• the amino group is -NH 2 , alkylamino, or dialkylamino.
• the aryl group is an optionally substituted phenyl.
• the alkyl is a C1-C6 alkyl. In another embodiment, the alkyl is a C 1 -C 4 alkyl.
• Non-limiting exemplary (amino)(aryl)alkyl groups include: [0740]
• the term "amino" as used by itself or as part of another group refers to a radical of the formula -NR 55a R 55b , wherein R 55a and R 55b are independently hydrogen, optionally substituted alkyl, haloalkyl, (hydroxy)alkyl, (alkoxy)alkyl, (amino)alkyl, heteroalkyl, optionally substituted cycloalkyl, optionally substituted heterocyclo, optionally substituted aryl, optionally substituted heteroaryl, (aryl)alkyl, (cycloalkyl)alkyl, (heterocyclo)alkyl, or (heteroaryl)alkyl
• the amino is -NH2.
• the amino is an "alkylamino," i.e., an amino group wherein R 55a is C1-6 alkyl and R 55b is hydrogen. In one embodiment, R 55a is C 1 -C 4 alkyl.
• Non-limiting exemplary alkylamino groups include -N(H)CH3 and -N(H)CH2CH3.
• the amino is a "dialkylamino,” i.e., an amino group wherein R 55a and R 55b are each independently C 1-6 alkyl. In one embodiment, R 55a and R 55b are each independently C 1 -C 4 alkyl.
• Non-limiting exemplary dialkylamino groups include -N(CH 3 ) 2 and -N(CH 3 )CH 2 CH(CH 3 ) 2 .
• the amino is a "hydroxyalkylamino," i.e., an amino group wherein R 55a is (hydroxyl)alkyl and R 55b is hydrogen or C 1 -C 4 alkyl.
• the amino is a "cycloalkylamino,” i.e., an amino group wherein R 55a is optionally substituted cycloalkyl and R 55b is hydrogen or C 1 -C 4 alkyl.
• the amino is a "aralkylamino," i.e., an amino group wherein R 55a is aralkyl and R 55b is hydrogen or C 1 -C 4 alkyl.
• Non-limiting exemplary aralkylamino groups include -N(H)CH2Ph, -N(H)CHPh2, and -N(CH3)CH2Ph.
• the amino is a "(cycloalkyl)alkylamino,” i.e., an amino group wherein R 55a is (cycloalkyl)alkyl and R 55b is hydrogen or C 1 -C 4 alkyl.
• Non-limiting exemplary (cycloalkyl)alkylamino groups include: [0748]
• the amino is a "(heterocyclo)alkylamino," i.e., an amino group wherein R 55a is (heterocyclo)alkyl and R 55b is hydrogen or C 1 -C 4 alkyl.
• Non- limiting exemplary (heterocyclo)alkylamino groups include: [0749]
• the term "(amino)alkyl" as used herein by itself or as part of another group refers to an alkyl substituted with one amino group.
• the amino group is - NH 2 .
• the amino group is an alkylamino.
• the amino group is a dialkylamino.
• the alkyl is a C1-C6 alkyl.
• the alkyl is a C 1 -C 4 alkyl.
• Non-limiting exemplary (amino)alkyl groups include -CH 2 NH 2 , CH 2 CH 2 N(H)CH 3 , -CH 2 CH 2 N(CH 3 ) 2 , CH 2 N(H)cyclopropyl, -CH 2 N(H)cyclobutyl, and -CH 2 N(H)cyclohexyl, and -CH 2 CH 2 CH 2 N(H)CH 2 Ph and -CH2CH2CH2N(H)CH2(4-CF3-Ph).
• the present disclosure encompasses any of the Compounds of the Disclosure being isotopically-labelled (i.e., radiolabeled) by having one or more atoms replaced by an atom having a different atomic mass or mass number.
• isotopes that can be incorporated into the disclosed compounds include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and chlorine, such as 2 H (or deuterium (D)), 3 H, 1 1 C, 13 C, 14 C, 15 N, 18 O, 17 O, 31 P, 32 P, 35 S, 18 F, and 36 Cl, respectively, e.g., 3 H, 11 C, and 14 C.
• compositions wherein substantially all of the atoms at a position within the Compound of the Disclosure are replaced by an atom having a different atomic mass or mass number.
• Isotopically-labelled Compounds of the Disclosure can be prepared by methods known in the art. [0751] Compounds of the Disclosure may contain one or more asymmetric centers and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms.
• the present disclosure encompasses the use of all such possible forms, as well as their racemic and resolved forms and mixtures thereof.
• the individual enantiomers can be separated according to methods known in the art in view of the present disclosure.
• the compounds described herein contain olefinic double bonds or other centers of geometric asymmetry, and unless specified otherwise, it is intended that they include both E and Z geometric isomers. All tautomers are also encompassed by the present disclosure.
• the term "stereoisomers” is a general term for all isomers of individual molecules that differ only in the orientation of their atoms in space.
• enantiomers and isomers of compounds with more than one chiral center that are not mirror images of one another include enantiomers and isomers of compounds with more than one chiral center that are not mirror images of one another (diastereomers).
• chiral center or "asymmetric carbon atom” refers to a carbon atom to which four different groups are attached.
• enantiomer and enantiomeric refer to a molecule that cannot be superimposed on its mirror image and hence is optically active wherein the enantiomer rotates the plane of polarized light in one direction and its mirror image compound rotates the plane of polarized light in the opposite direction.
• racemic refers to a mixture of equal parts of enantiomers and which mixture is optically inactive.
• Compounds of the Disclosure are racemic.
• absolute configuration refers to the spatial arrangement of the atoms of a chiral molecular entity (or group) and its stereochemical description, e.g., R or S.
• stereochemical terms and conventions used in the specification are meant to be consistent with those described in Pure & Appl. Chem 68:2193 (1996), unless otherwise indicated.
• enantiomeric excess or “ee” refers to a measure for how much of one enantiomer is present compared to the other.
• the percent enantiomeric excess is defined as ([ ⁇ ]obs/[ ⁇ ]max)*100, where [ ⁇ ]obs is the optical rotation of the mixture of enantiomers and [ ⁇ ]max is the optical rotation of the pure enantiomer. Determination of enantiomeric excess is possible using a variety of analytical techniques, including NMR spectroscopy, chiral column chromatography or optical polarimetry.
• Compounds of the Disclosure having one or more chiral centers are enantiomerically enriched, e.g., the ee is about 5% or more. In another embodiment, the ee is about 10%. In another embodiment, the ee is about 20%. In another embodiment, the ee is about 30%. In another embodiment, the ee is about 40%. In another embodiment, the ee is about 50%. In another embodiment, the ee is about 60%. In another embodiment, the ee is about 70%. In another embodiment, the ee is about 80%. In another embodiment, the ee is about 85%. In another embodiment, the ee is about 90%. In another embodiment, the ee is about 91%.
• the ee is about 92%. In another embodiment, the ee is about 93%. In another embodiment, the ee is about 94%. In another embodiment, the ee is about 95%. In another embodiment, the ee is about 96%. In another embodiment, the ee is about 97%. In another embodiment, the ee is about 98%. In another embodiment, the ee is about 99%. [0760]
• the terms “a” and “an” refer to one or more.
• the term "about,” as used herein, includes the recited number ⁇ 10%. Thus, "about 10" means 9 to 11.
• treat refers to eliminating, reducing, or ameliorating a disease or condition, and/or symptoms associated therewith. Although not precluded, treating a disease or condition does not require that the disease, condition, or symptoms associated therewith be completely eliminated.
• the terms “treat,” “treating,” “treatment,” and the like may include “prophylactic treatment,” which refers to reducing the probability of redeveloping a disease or condition, or of a recurrence of a previously-controlled disease or condition, in a subject who does not have, but is at risk of or is susceptible to, redeveloping a disease or condition or a recurrence of the disease or condition.
• treatment also includes relapse prophylaxis or phase prophylaxis, as well as the treatment of acute or chronic signs, symptoms and/or malfunctions.
• the treatment can be orientated symptomatically, for example, to suppress symptoms. It can be effected over a short period, be oriented over a medium term, or can be a long-term treatment, for example within the context of a maintenance therapy.
• terapéuticaally effective amount refers to an amount of the active ingredient(s) that is(are) sufficient, when administered by a method of the disclosure, to efficaciously deliver the active ingredient(s) for the treatment of condition or disease of interest to an individual in need thereof.
• the therapeutically effective amount of the agent may reduce (i.e., retard to some extent and preferably stop) unwanted cellular proliferation; reduce the number of cancer cells; reduce the tumor size; inhibit (i.e., retard to some extent and preferably stop) cancer cell infiltration into peripheral organs; inhibit (i.e., retard to some extent and preferably stop) tumor metastasis; inhibit, to some extent, tumor growth; modulate protein methylation in the target cells; and/or relieve, to some extent, one or more of the symptoms associated with the cancer.
• the administered compound or composition prevents growth and/or kills existing cancer cells, it may be cytostatic and/or cytotoxic.
• the term “container” means any receptacle and closure therefore suitable for storing, shipping, dispensing, and/or handling a pharmaceutical product.
• the term “insert” means information accompanying a pharmaceutical product that provides a description of how to administer the product, along with the safety and efficacy data required to allow the physician, pharmacist, and patient to make an informed decision regarding use of the product.
• the package insert generally is regarded as the "label” for a pharmaceutical product.
• the term “disease” or “condition” or “disorder” denotes disturbances and/or anomalies that as a rule are regarded as being pathological conditions or functions, and that can manifest themselves in the form of particular signs, symptoms, and/or malfunctions.
• Compounds of the Disclosure inhibit SETD2 protein and can be used in treating diseases and conditions such as proliferative diseases, wherein inhibition of SETD2 protein provides a benefit. See, e.g., U.S. Provisional Appl. No.62/545,353.
• the Compounds of the Disclosure can be used to treat a "SETD2 protein mediated disorder"
• a SETD2 protein mediated disorder is any pathological condition in which a SETD2 protein is known to play a role.
• a SETD2 mediated disorder is a proliferative disease.
• inhibiting SETD2 protein is the inhibition of the activity of one or more activities of SETD2 protein.
• the activity of the SETD2 protein is the ability of the SETD2 protein to transfer a methyl group to a target protein, e.g., histone. It should be appreciated that the activity of SETD2 may be inhibited in vitro or in vivo. Exemplary levels of inhibition of the activity of SETD2 include at least 5% inhibition at least 10% inhibition, at least 20% inhibition, at least 30% inhibition, at least 40% inhibition, at least 50% inhibition, at least 60% inhibition, at least 70% inhibition, at least 80% inhibition, at least 90% inhibition, and up to about 100% inhibition. [0770]
• the term "biological sample” as used herein refers any tissue or fluid from a subject that is suitable for detecting chromosomal translocations.
• useful biological samples include, but are not limited to, biopsied tissues and/or cells, e.g., solid tumor, lymph gland, inflamed tissue, tissue and/or cells involved in a condition or disease, blood, plasma, serous fluid, cerebrospinal fluid, saliva, urine, lymph, cerebral spinal fluid, and the like.
• Other suitable biological samples will be familiar to those of ordinary skill in the relevant arts.
• a biological sample can be analyzed for chromosomal translocations using any technique known in the art. Such techniques include, but are not limited to, polymerase chain reaction (PCR) methodology, reverse transcription-polymerase chain reaction (RT-PCR) methodology, or cytoplasmic light chain immunofluorescence combined with fluorescence in situ hybridization (cIg-FISH).
• PCR polymerase chain reaction
• RT-PCR reverse transcription-polymerase chain reaction
• cIg-FISH cytoplasmic light chain immunofluorescence combined with fluorescence in situ hybridization
• a biological sample can be obtained using techniques that are well within the scope of ordinary knowledge of a clinical practioner.
• the biological sample comprises blood cells.
• the phrase "in combination" as used in connection with the administration of a Compound of the Disclosure and a Second Therapeutic Agent to a subject means that the Compound of the Disclosure and the Second Therapeutic Agent can be administered to the subject together, e.g., as part of a single pharmaceutical composition or formulation, or separately, e.g., as part of two or more separate pharmaceutical compositions or formulations.
• the phrase "in combination" as used in connection with the administration of a Compound of the Disclosure and a Second Therapeutic Agent to a subject is thus intended to embrace administration of a Compound of the Disclosure and a Second Therapeutic Agent in a sequential manner, wherein the Compound of the Disclosure and the Second Therapeutic Agent are administered to the subject at a different time, as well as administration concurrently, or in a substantially simultaneous manner.
• Simultaneous administration can be accomplished, for example, by administering to the subject a single capsule having a fixed ratio of each of the Compound of the Disclosure and the Second Therapeutic Agent or in multiple, single capsules for each of the Compound of the Disclosure and the Second Therapeutic Agent.
• Sequential or substantially simultaneous administration of the Compound of the Disclosure and the Second Therapeutic Agent agent can be accomplished by any appropriate route including, but not limited to, oral routes, intravenous routes, intramuscular routes, and direct absorption through mucous membrane tissues.
• the Compound of the Disclosure and the Second Therapeutic Agent can be administered by the same route or by different routes.
• the Second Therapeutic Agent of the combination may be administered by intravenous injection while the Compound of the Disclosure of the combination may be administered orally.
• both the Compound of the Disclosure and the Second Therapeutic Agent may be administered orally or both the Compound of the Disclosure and the Second Therapeutic Agent may be administered by intravenous injection.
• the Compound of the Disclosure and the Second Therapeutic Agent may also be administered in alternation.
• the Compound of the Disclosure and the Second Therapeutic Agent are administered to a subject separately, e.g., as part of two or more separate pharmaceutical compositions or formulations.
• Compounds of the Disclosure are prepared using methods disclosed in PCT/US2019/046569, or by the illustrative methods shown in the General Schemes below. In the General Schemes, R 1d , R 2b , R 2d , R 2e , A 1 , A 2 , R 11a , R 14a , R 14b , R 19 , R 20 , G, Z 4 , and q are as defined in connection with Formulae II, III, IV, V, or VI, unless otherwise indicated.
• suitable protecting groups can be employed in the synthesis, for example, when Z is (amino)alkyl or any other group that may group that may require protection, or when R 8 is amino, (amino)alkyl, or any other group that may require protection.
• R 8 is amino, (amino)alkyl, or any other group that may require protection.
• step 2 the compound of Formula (2) is converted to the indole of Formula (3) under acidic conditions.
• step 3 the compound of Formula (3) is hydrolyzed to give the indole-2-carboxylic acid of Formula (4).
• step 4 a compound of Formula (4) is reacted with G 1 NH 2 under standard coupling conditions to give a compound of Formula II.
• General Scheme 1 Formula II [0775] In General Scheme 2, a compound of Formula (5) is reacted with R 2b -H wherein R 2b is a heterocyclo, e.g., R 2b -H is piperidine, or an amine, e.g., R 2b -H is dimethyl amine, to give a compound of Formula (6).
• step 3 the compound of Formula (7) is reacted with a compound of Formula (4), see General Scheme 1, under standard coupling conditions to give a compound of Formula III, wherein A 1 and A 2 are CH and R 2b is an optionally substituted heterocyclo or an amino group.
• step 3 Formula IV (wherein R 11a is optionally substituted heterocyclo; and Z 5 is CH 2 )
• step 1 of General Scheme 5 a nitrile of Formula (14) is reacted with a Grignard reagent (R 14a -MgBr) and the resulting product is reduced to give a compound of Formula (15).
• the compound of Formula (15) is coupled with a compound of Formula (4) to give a compound of Formula V, wherein p is 0.
• General Scheme 5 [0779]
• an aldehyde of Formula (16) is reacted with an ester of Formula (17) to give a compound of Formula (18).
• step 2 the compound of Formula (18) hydrolyzed to give a compound of Formula (19).
• step 3 the compound of Formula (19) is converted to the isocyanate of Formula (20).
• the compound of Formula (20) is reacted with benzyl alcohol to give a compound of Formula (21).
• Hydrogenation of a compound of Formula (21) and removal of the Cbz groups gives an amine of Formula (23).
• Coupling a compound of Formula (23) with a compound of Formula (4) gives a compound of Formula V, wherein p is 1.
• General Scheme 6 Formula V (wherein p is 1) [0780]
• the nitrile of Formula (24) is reduced to give an amine of Formula (25).
• the compound of Formula (25) is coupled with a compound of Formula (4) to give a compound of Formula VI.
• the resulting solution was stirred for 2 h at 25 o C.
• the reaction progress was monitored by LCMS, and the reaction solution was quenched by 10 ml of water.
• the resulting solution was extracted with 3x15 ml of ethyl acetate and the organic layers combined and concentrated under vacuum. The residue was applied onto a silica gel column with ethyl acetate/petroleum ether (1:1).
• the product thus obtained was further purified by Prep-HPLC with the following conditions: Column, XBridge Prep Phenyl OBD Column, 5 ⁇ m, 19*150 mm; mobile phase, Water with 10 mmol TFA and MeCN (20.0% MeCN up to 30.0% in 10 min, up to 95.0% in 1 min, hold 95.0% in 1 min, down to 20.0% in 2 min); Detector, UV 254/220 nm.
• Quantification of the effect of single agents or combinations on cell viability was performed through measurement of cellular adenosine triphosphate (ATP) using a CellTiter-Glo® (Promega, Madison, WI) Luminescent Cell Viability Assay. Luminescence was detected using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Concentration response plots were generated in GraphPad Prism version 7.0 for Windows, GraphPad Software, (La Jolla, California) and curves fitted to a four-parameter logistic model with variable slope. Percent of inhibition was calculated at each treatment concentration.
• NCI-H929, MM1.S, MINO, REC1, MAVER1, Z138, JEKO1, JVM2, and RPMI-8226 cell lines were purchased from the American Type Culture Collection (ATCC, Manassas VA); KMS-11, KMS34, and KMS-28-BM were purchased from the Japanese Collection of Research Bioresources (JCRB, Osaka, Japan); and L-363 and GRANTA519 were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures. [0794] The results of these combinations are summarized in Tables A and B (multiple myeloma cell lines), and Tables C and D (mantle cell lymphoma cell lines).
• Fig. 3 shows the synergistic anti-proliferative activity of Cpd. No. 15 combined with pomalidomide in KMS-11 cells in a 7-day co-treatment study.
• Fig. 4 shows the Loewe Model analysis of the Cpd. No. 15/pomalidomide combination studies in KMS-11 cells.
• Diffuse large B-cell lymphoma cells were seeded into flasks and pre-treated with several concentrations of Cpd. No.15 or DMSO. Cells were then split adjusted to initial density, replated and co-treated Cpd. No.
• Quantification of the effect of single agents or combinations on cell viability was performed through measurement of cellular adenosine triphosphate (ATP) using CellTiter-Glo® (Promega, Madison, WI). Luminescence was detected using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Concentration response plots were generated in GraphPad Prism version 8.0 for Windows, GraphPad Software, (La Jolla, California) and curves fitted to a four-parameter logistic model with variable slope. Percent of inhibition was calculated at each treatment concentration.
• WSUDLCL2 was purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures and KARPAS422 was purchased from Sigma Aldrich (St. Louis, MO). The results of these combinations are summarized in Table E (diffuse large B-cell lymphoma cell lines).

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