WO2021166102A1 - Solution saccharifiée - Google Patents

Solution saccharifiée Download PDF

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Publication number
WO2021166102A1
WO2021166102A1 PCT/JP2020/006443 JP2020006443W WO2021166102A1 WO 2021166102 A1 WO2021166102 A1 WO 2021166102A1 JP 2020006443 W JP2020006443 W JP 2020006443W WO 2021166102 A1 WO2021166102 A1 WO 2021166102A1
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WIPO (PCT)
Prior art keywords
saccharified solution
cellulose
content
saccharified
containing biomass
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PCT/JP2020/006443
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English (en)
Japanese (ja)
Inventor
敦 古城
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王子ホールディングス株式会社
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Application filed by 王子ホールディングス株式会社 filed Critical 王子ホールディングス株式会社
Priority to PCT/JP2020/006443 priority Critical patent/WO2021166102A1/fr
Priority to PCT/JP2020/013246 priority patent/WO2021166273A1/fr
Priority to PCT/JP2021/006346 priority patent/WO2021167064A1/fr
Priority to TW110105795A priority patent/TW202144581A/zh
Publication of WO2021166102A1 publication Critical patent/WO2021166102A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides

Definitions

  • the present invention relates to a saccharified solution. Specifically, the present invention relates to a saccharified solution using cellulose-containing biomass as a raw material.
  • Sugar may be used as a fermentation raw material.
  • a saccharified solution containing monosaccharides such as six-carbon sugar and five-carbon sugar is assimilated by yeast and converted into ethanol.
  • the ethanol thus obtained is effectively used as, for example, ethanol for fuel or ethanol for beverages.
  • starch derived from edible raw materials such as sugar beet, potatoes, and corn is industrially used.
  • the process of obtaining saccharified liquid from non-edible raw materials such as trees and grass and further producing chemical products such as ethanol by fermentation reaction. Is required to be built.
  • cellulose-containing biomass is hydrolyzed using an enzyme or a microorganism that produces the enzyme to decompose cellulose and hemicellulose contained in the biomass to produce sugar.
  • Patent Document 1 describes a step of hydrolyzing cellulose-containing biomass with cellulase derived from filamentous fungi, filtering the hydrolyzate with an ultrafiltration membrane, recovering cellulase as a non-permeate, and using a saccharified solution as a permeate.
  • a method for producing a saccharified solution containing glucose, a xylooligo, or the like, which comprises a step of recovering and a step of allowing the recovered cellulase to act on a xylan-containing raw material, is disclosed.
  • Patent Document 2 describes a step of reacting a mannanase with a liquid component obtained by hydrotreating woody biomass to obtain a saccharified solution, and a microfiltration membrane and / or an ultrafiltration membrane of the saccharified solution.
  • a method for producing a sugar solution including a step of filtering and recovering the sugar solution from the permeate side is disclosed.
  • Patent Document 3 describes a step of hydrolyzing a cellulose-containing biomass to produce a sugar aqueous solution, and filtering the obtained sugar aqueous solution through a nanofiltration membrane and / or a reverse osmosis membrane from the non-permeable side.
  • a method for producing a sugar solution including a step of recovering a purified sugar solution and removing a fermentation inhibitor such as furfural from the permeation side is disclosed.
  • a saccharified solution is obtained from starch derived from edible raw materials such as tensai, potatoes, and corn
  • the starch degrading enzyme (amylase) cannot cut the branched structural part of the starch, so oligosaccharides are contained in the obtained saccharified solution. Will be included.
  • the saccharified solution there is a desire to obtain a high-concentration glucose solution, but if the saccharified solution contains oligosaccharides, the purity of glucose will decrease, and the efficiency of the subsequent fermentation process will be reduced. There is a problem that it decreases.
  • the conventional method may not sufficiently remove fermentation inhibitors such as furfural, or enzymes used in the saccharification reaction may be mixed in the saccharified solution. there were.
  • the presence of a fermentation inhibitor such as furfural poses a problem because it reduces the efficiency of the subsequent fermentation step.
  • the enzyme used for the saccharification reaction is mixed in the saccharified solution, the purity of glucose is lowered and the reuse efficiency of the enzyme is lowered, which is a problem.
  • the present inventors have prepared a saccharified solution having a small residual amount of enzyme and capable of increasing fermentation efficiency when subjected to a fermentation step. We proceeded with the study for the purpose of providing it.
  • the present inventors used cellulose-containing biomass as a raw material, and in a saccharified solution having an electrical conductivity of 500 ⁇ S / cm or more, the oligosaccharide content and the content of furfural were found. It has been found that by setting the content and the content of soluble lignin as predetermined conditions, a saccharified solution having a small residual amount of enzyme and capable of increasing fermentation efficiency when subjected to a fermentation step can be obtained.
  • the present invention has the following configuration.
  • the electrical conductivity of the saccharified solution is 500 ⁇ S / cm or more
  • a saccharified solution in which the oligosaccharide content in the saccharified solution is 10 ppm or less, the furfural content is 1 ppm or less, and the soluble lignin content is 100 ppm or more.
  • the saccharified solution according to [1] wherein the content of lignin in the cellulose-containing biomass is 1% by mass or more and 3% by mass or less.
  • the present invention may have the following configurations.
  • the electrical conductivity of the saccharified solution is 500 ⁇ S / cm or more
  • the oligosaccharide content in the saccharified solution is 10 ppm or less
  • the furfural content is 1 ppm or less
  • the soluble lignin content is 120 ppm or more.
  • a method for producing a saccharified liquid which comprises a step of membrane-treating the treatment liquid obtained in the saccharification treatment step with an ultrafiltration membrane.
  • the electrical conductivity of the saccharified solution is 500 ⁇ S / cm or more
  • the oligosaccharide content in the saccharified solution is 10 ppm or less
  • the furfural content is 1 ppm or less
  • the soluble lignin content is 120 ppm or more.
  • a method for producing a saccharified solution, wherein the enzymatic activity of the saccharified solution is 10 U / ml or less.
  • the present invention relates to a saccharified solution using cellulose-containing biomass as a raw material.
  • the electrical conductivity of the saccharified solution is 500 ⁇ S / cm or more.
  • the oligosaccharide content in the saccharified solution is 10 ppm or less, the furfural content is 1 ppm or less, and the soluble lignin content is 100 ppm or more.
  • the electric conductivity of the saccharified solution is 500 ⁇ S / cm or more, which means that the saccharified solution is a crude saccharified solution. That is, the present invention relates to a crude saccharified solution having an electrical conductivity of 500 ⁇ S / cm or more, in which the contents of oligosaccharide, furfural and soluble lignin are within a predetermined range.
  • the saccharified solution of the present invention has the above-mentioned structure, the residual amount of the enzyme in the saccharified solution is kept low. Specifically, by setting the content of soluble lignin in the saccharified solution of the present invention to 100 ppm or more, the residual amount of the enzyme in the saccharified solution can be suppressed to a low level.
  • cellulose-containing biomass contains lignin
  • lignin contains soluble lignin and insoluble lignin in a certain ratio. And most of the enzymes used in the saccharification step are recovered together with insoluble lignin.
  • the fact that the content of soluble lignin in the saccharified solution is equal to or higher than a predetermined value means that a certain amount of insoluble lignin was present, and that the enzyme was sufficiently recovered after the saccharification step. It is suggested. Therefore, it is considered that the residual amount of the enzyme in the saccharification solution can be kept low by controlling the starting material and the saccharification step so that the content of soluble lignin in the saccharification solution is 100 ppm or more.
  • the enzyme activity of the saccharified solution is preferably 10 U / ml or less, and more preferably 5 U / ml or less.
  • the enzyme activity of the saccharified solution is a value measured by the following method. First, 4 ml of 100 mM acetate buffer (pH 5) and 32 ml of 1.25 mM 4-methylhumberiferyl ⁇ -D glucoside (manufactured by Wako Co., Ltd.) are added to 4 ml of the saccharified solution, and the mixture is reacted at 37 ° C. for 30 minutes.
  • the reaction is stopped with 200 ml of a 0.5 M glycine-NaOH solution (pH 10.5), and the fluorescence intensity (excitation wavelength 355 nm, measurement wavelength 460 nm) is measured with a fluorometer (Infinite 200 manufactured by Tecan Co., Ltd.).
  • the enzyme residual activity is calculated assuming that the enzyme residual activity when 1 mM 4-methylumbelliferone is released per 1 ml of the saccharified solution is 1 U / ml.
  • the saccharified solution of the present invention has the above-mentioned structure, the fermentation efficiency of the saccharified solution can be increased when the saccharified solution is subjected to a fermentation step.
  • the saccharified solution may be used as a fermentation raw material.
  • a saccharified solution containing a monosaccharide such as hexasaccharide or pentasaccharide is assimilated by yeast and converted into ethanol.
  • the ethanol thus obtained is used as, for example, ethanol for fuel or ethanol for beverages.
  • the fermentation efficiency when fermenting the saccharified solution can be increased, and as a result, the ethanol yield can be increased.
  • the ethanol yield in the present specification is a value calculated as the amount of ethanol produced per unit cellulose-containing biomass amount (kg) by measuring the amount of ethanol in the obtained fermented liquid by fermenting the saccharified liquid by the method described later. be. Specifically, 2 ml of corn steep liquor (manufactured by Oji Cornstarch) and 5 ml of 1M acetate buffer (pH 5) were added to 50 ml of the saccharified solution, and 5 ml of the pre-cultured yeast solution (Saccharomyces cerevisiae 1 ⁇ 10 8 cells / mL) was added. Add and hold at 33 ° C. for 18 hours.
  • the amount of ethanol in the culture solution after the reaction is measured using a high performance liquid chromatography device (HP-2200 manufactured by Azilent Co., Ltd.), and the amount of ethanol produced per unit cellulose-containing biomass amount (kg) is calculated.
  • the ethanol yield calculated in this way is preferably 510 mL / kg or more, more preferably 530 mL / kg or more, and even more preferably 540 mL / kg or more.
  • the oligosaccharide content in the saccharified solution of the present invention may be 10 ppm or less, preferably 5 ppm or less, and more preferably 1 ppm or less.
  • the oligosaccharide content in the saccharified solution is particularly preferably 0 ppm.
  • the oligosaccharide refers to one in which about 2 to 10 monosaccharides are bound by glycosidic bonds. Examples of monosaccharides include glucose, galactose, mannose, fructose and the like.
  • the oligosaccharide content can be measured by ion chromatography. In ion chromatography, the oligosaccharide content is detected as a peak observed after the monosaccharide elutes and can be quantified by a calibration curve.
  • the content of monosaccharide in the saccharified solution of the present invention is preferably 1% by mass or more, more preferably 3% by mass or more, and further preferably 5% by mass or more.
  • the content of the monosaccharide in the saccharified solution is preferably 50% by mass or less, more preferably 30% by mass or less, and further preferably 20% by mass or less.
  • the content of monosaccharides in the saccharified solution can be quantified by detecting the peak of monosaccharides by ion chromatography.
  • the saccharified solution of the present invention contains glucose. Further, since the saccharified solution of the present invention is made from cellulose-containing biomass, it contains other sugars in addition to glucose. Other sugars include, for example, xylose, arabinose, mannose, xylose, galactose.
  • the saccharified solution of the present invention is a saccharified solution made from cellulose-containing biomass. Whether or not the obtained saccharified solution is made from cellulose-containing biomass can be determined by, for example, whether or not the saccharified solution contains lignin, and the lignin in the saccharified solution can be determined. With the detection, it can be determined that the raw material is cellulose-containing biomass.
  • the cellulose-containing biomass is preferably a tree-derived raw material
  • the saccharified solution of the present invention is preferably a saccharified solution obtained from a tree-derived raw material. Examples of the tree-derived raw material include eucalyptus, acacia, pine, birch, spruce, sugi, and cypress.
  • the cellulose-containing biomass contains lignin, and the content of lignin in the cellulose-containing biomass is preferably 1% by mass or more, more preferably 1.5% by mass or more, and 2% by mass or more. Is even more preferable.
  • the content of lignin in the cellulose-containing biomass is preferably 3% by mass or less.
  • the amount of soluble lignin remaining in the saccharified solution may be 100 ppm or more, preferably 120 ppm or more, and more preferably 150 ppm or more.
  • the upper limit of the amount of soluble lignin remaining in the saccharified solution is not particularly limited, but is preferably 2000 ppm or less, for example.
  • the soluble lignin content is a value calculated by the following formula by measuring the absorbance of the UV spectrum (wavelength 210 nm) of the saccharified solution.
  • Soluble lignin content (ppm) dilution ratio x amount of saccharified solution x sample solution extinction / coefficient A x amount of solids in saccharified solution
  • coefficient A is the extinction coefficient of lignin (110 L / g / cm).
  • the saccharified solution of the present invention is a saccharified solution using cellulose-containing biomass as a raw material, and can be obtained by saccharifying the cellulose-containing biomass.
  • the reaction between the water and the cellulose-containing biomass is carried out in the presence of an enzyme or a microorganism that produces the enzyme. Therefore, the content of furfural contained in the obtained saccharified solution is 1 ppm or less.
  • the content of furfural contained in the saccharified solution is measured by high performance liquid chromatography.
  • the present invention may relate to a purified saccharified solution obtained by purifying the above-mentioned saccharified solution.
  • the purified saccharified solution can be obtained by treating the above-mentioned saccharified solution with an ion exchange resin.
  • an ion exchange resin 10 g of an ion exchange resin (Amber Jet 1024, IRA96SB, manufactured by Organo Corporation) is added to 100 ml of the above-mentioned saccharified solution, and the mixture is stirred at 1 room temperature for 30 minutes. Then, by performing filtration, the filtrate is separated from the ion exchange resin, and the obtained filtrate becomes a purified saccharified solution.
  • the electrical conductivity of the purified saccharified solution is preferably 10 ⁇ S / cm or less, preferably 8 ⁇ S / cm or less, and preferably 7 ⁇ S / cm or less.
  • the electrical conductivity of the purified saccharified solution is preferably 5 ⁇ S / cm or more.
  • the content of soluble lignin in the purified saccharified solution is preferably 10 ppm or less, more preferably 9 ppm or less, and even more preferably 8 ppm or less.
  • the electrical conductivity of the purified saccharified solution is preferably 4 ppm or more.
  • the monosaccharide content, oligosaccharide content, and furfural content in the purified saccharified solution are almost the same as those in the above-mentioned saccharified solution because they hardly change before and after the treatment with the ion exchange resin.
  • the method for producing a saccharified solution of the present invention includes a step of saccharifying cellulose-containing biomass.
  • Biomass is a biological resource excluding fossil fuels, and examples of the cellulose-containing biomass include biological resources containing a cellulose component.
  • biological resources containing cellulose components include papermaking trees, forest residue, chips or bark of thinned lumber, sawdust or sawdust generated from sawmills, pruned branches and leaves of roadside trees, construction waste, and the like. Be done.
  • herbaceous resources include agricultural wastes such as kenaf, rice straw, straw, and bagasse, and herbaceous energy crops such as Elianthus, Miscanthus, and Napiergrass.
  • wood-derived paper, used paper, pulp, pulp sludge and the like can also be used.
  • the cellulose-containing biomass used in the present invention is preferably a tree-derived raw material.
  • Cellulose is a chain polymer compound formed by the ⁇ -1,4 glycoside bond between a large number of glucose molecules.
  • cellulose a large number of glucoses are bound by dehydration condensation of the 1-position hydroxyl group of one glucose and the 4-position hydroxyl group of another glucose.
  • 1,4-glycosidic bonds are cleaved, and generally, monosaccharides and oligosaccharides in which about 2 to 10 monosaccharides are bound are produced.
  • the cellulose-containing biomass also includes hemicellulose, which is a polysaccharide existing between cellulose microfibrils. Therefore, when cellulose-containing biomass is saccharified, the hydrolyzate includes glucose, which is a cellulose-derived saccharide, and xylose, arabinose, mannose, and the like, which are hemicellulose-derived saccharides.
  • the method for producing a saccharified solution of the present invention includes a step of saccharifying a broadleaf kraft pulp or a softwood kraft pulp having a lignin content of 1% by mass or more and 3% by mass or less by mixing water and cellulase, and saccharification. It is preferable to include a step of membrane-treating the treatment liquid obtained in the step of treatment with an ultrafiltration membrane.
  • the electrical conductivity of the saccharified solution is 500 ⁇ S / cm or more
  • the oligosaccharide content in the saccharified solution is 10 ppm or less
  • the furfural content is 1 ppm or less
  • the soluble lignin content is 120 ppm or more.
  • the enzymatic activity of the saccharified solution is 10 U / ml or less.
  • the saccharification treatment step is a step of hydrolyzing the polysaccharide contained in the cellulose-containing biomass to obtain a monosaccharide.
  • the reaction with the cellulose-containing biomass is carried out in the presence of water and an enzyme and / or a microorganism producing the enzyme, and stirring is preferably carried out.
  • the solid content concentration in the stirring liquid is preferably 1 to 30% by mass, although it depends on the type of enzyme.
  • the temperature of the stirring liquid is preferably 30 to 75 ° C., and the pH of the stirring liquid is preferably 3.0 to 7.0.
  • the saccharification treatment time is preferably 2 to 200 hours.
  • the enzyme used in the saccharification treatment step is preferably a cellulolytic enzyme.
  • Cellulose-degrading enzyme is an enzyme having at least one selected from cellobiohydrolase activity, endoglucanase activity and beta-glucosidase activity, and is an enzyme generically called cellulase.
  • the cellulase may have hemicellulase activity.
  • Cellulase a commercially available cellulase preparation can be used.
  • Cellulase preparations include the genus Trichoderma, the genus Acremonium, the genus Aspergillus, the genus Fanerochaete, the genus Trametes, the genus Trametes, the genus Humicola, and the genus Humicola. Examples of cellulase preparations to be used.
  • cellulase T2 manufactured by HPI
  • Meiserase manufactured by Meiji Seika
  • Novozymes 188 manufactured by Novozymes
  • Multifect CX10L manufactured by Genecore
  • GC220 manufactured by Genecore
  • the amount of the cellulase preparation used with respect to 100 parts by mass of the cellulose-containing biomass is preferably 0.5 to 100 parts by mass, and more preferably 1 to 50 parts by mass.
  • the method for producing a saccharified liquid of the present invention preferably includes a step of membrane-treating the treatment liquid obtained in the saccharification treatment step.
  • UF membranes Materials for ultrafiltration (UF) membranes include polyethersulfone (PES), polysulfone (PS), polyacrylonitrile (PAN), polyvinylidene fluoride (PVDF), regenerated cellulose, cellulose, cellulose ester, sulfonated polysulfone, sulfone.
  • PES polyethersulfone
  • PS polysulfone
  • PAN polyacrylonitrile
  • PVDF polyvinylidene fluoride
  • regenerated cellulose cellulose, cellulose ester
  • modified polyether sulfone polyolefin
  • polyvinyl alcohol polymethylmethacrylate
  • polytetrafluoroethylene polytetrafluoroethylene
  • a ceramic filter or MF film may be used. Further, in the process of membrane treatment, a filtration method using a filter press or a centrifugation treatment method can also be adopted.
  • the membrane treatment method adopted in the membrane treatment step is not particularly limited, but for example, a simple filtration method (total filtration method) in which treated water is supplied in a direction perpendicular to the membrane surface and a membrane surface.
  • a simple filtration method total filtration method
  • cross-flow filtration in which treated water is supplied in a parallel direction can be mentioned.
  • the membrane permeate obtained in the process of membrane treatment contains a saccharified solution
  • the non-permeate side contains enzymes, raw material residues and the like.
  • the enzyme contained on the non-permeable side can be reused in the above-mentioned saccharification treatment step. By increasing the recycling efficiency of the enzyme, it is possible to suppress the production cost of the saccharified solution.
  • a step of pretreating the cellulose-containing biomass Before the saccharification treatment step, a step of pretreating the cellulose-containing biomass may be provided. Since the cellulose-containing biomass contains lignin, protein, and the like, pretreatment may be performed in order to improve the hydrolysis efficiency by cellulase. Examples of pretreatment methods include acid treatment with sulfuric acid and acetic acid, alkali treatment with caustic soda and ammonia, hydrothermal treatment, subcritical water treatment, steaming treatment, and chemical pulping treatment (sulfite cooking, craft cooking, etc.). Be done.
  • cellulose-containing biomass can be mechanically treated to increase the hydrolysis efficiency by cellulase.
  • the mechanical treatment include mechanical means such as cutting, cutting, crushing, and grinding.
  • the mechanical device to be used is not particularly limited, and for example, a cutting device, a uniaxial crusher, a biaxial crusher, a hammer crusher, a refiner, a kneader, a ball mill and the like can be used.
  • a foreign matter removing step may be provided to remove foreign matter (foreign matter such as stone, dust, metal, plastic, etc.).
  • the foreign matter removing step include a cleaning step.
  • a method for cleaning the cellulose-containing biomass for example, a method of spraying water on the cellulose-containing biomass to remove foreign substances mixed in the cellulose-containing biomass, or a method of immersing the cellulose-containing biomass in water to settle and remove the foreign substances. The method and the like can be mentioned.
  • Another method is to separate foreign matter from cellulose-containing biomass by using a device such as a metal trap or a washing drainer.
  • a sterilization treatment step may be provided as a pretreatment step. If germs are mixed in the cellulose-containing biomass, the germs may consume sugar in the saccharification treatment step and the yield of the product may decrease.
  • the sterilization treatment step may be a method of exposing the raw material to a pH at which bacteria are difficult to grow, such as acid or alkali, but a method of treating at a high temperature may be adopted.
  • a step of concentrating the membrane permeate may be provided.
  • the concentration of the saccharified solution contained in the membrane permeate is increased.
  • a concentrated saccharified solution can be obtained by heating the membrane permeate to evaporate the water content.
  • the saccharified solution of the present invention is preferably used as a fermentation raw material. Therefore, the saccharified solution of the present invention is subjected to a fermentation step and becomes a fermented solution.
  • the fermentation broth is particularly preferably ethanol. Since the content of oligosaccharide in the saccharified solution of the present invention is 10 ppm or less and the content of furfural is 1 ppm or less, fermentation efficiency is good when fermenting the saccharified solution. Therefore, when a fermented liquid (ethanol) is produced using the saccharified liquid of the present invention, a high ethanol yield is achieved.
  • the saccharified solution of the present invention has the above-mentioned structure, the ethanol purity of the fermentation broth (ethanol) is high and the content of impurities can be reduced. Therefore, for example, when ethanol is used for beverages. Can suppress miscellaneous taste and the like.
  • Example 1 ⁇ Saccharification treatment> To 80 mL of water, 5 g (dry weight) of oxygen-bleached hardwood kraft pulp having a lignin content of 2% by mass was added and stirred to prepare a pulp slurry. Next, 1N sulfuric acid was added to this pulp slurry to adjust the pH to 5.0, and 2 mL of a cellulase solution (Multifect CX10L manufactured by Genencore) and water were added to prepare the final volume to 100 mL. .. The solution was allowed to stand at 50 ° C. for 48 hours, and then the supernatant was filtered through an ultrafiltration membrane (Centricon Plus 70 centrifugal filter unit manufactured by Millipore) to obtain a saccharified solution.
  • a cellulase solution Multifect CX10L manufactured by Genencore
  • Example 2 A saccharified solution was obtained in the same manner as in Example 1 except that 5 g (dry weight) of oxygen-bleached softwood kraft pulp having a lignin content of 2% by mass was used as a raw material used for the saccharification treatment.
  • Example 1 A saccharified solution was obtained in the same manner as in Example 1 except that 5 g (dry weight) of bleached hardwood kraft pulp having a lignin content of 0.3% by mass was used as a raw material used for the saccharification treatment.
  • Example 2 A saccharified solution was obtained in the same manner as in Example 1 except that 5 g (dry weight) of bleached softwood kraft pulp having a lignin content of 0.3% by mass was used as a raw material used for the saccharification treatment.
  • ⁇ Furfural content> The content of furfural contained in the saccharified solution was measured using a high performance liquid chromatography device (HP-2200, manufactured by Agilent).
  • Soluble lignin content The absorbance of the UV spectrum (wavelength 210 nm) of the saccharified solution was measured, and the soluble lignin content was calculated by the following formula.
  • Soluble lignin content (ppm) dilution ratio x amount of saccharified solution x sample solution extinction / coefficient A x amount of solids in saccharified solution
  • coefficient A is the extinction coefficient of lignin (110 L / g / cm).

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Abstract

L'invention a pour objet de fournir une solution saccharifiée présentant une faible quantité de résidu d'enzymes, et permettant d'augmenter l'efficacité de fermentation lorsqu'elle est soumise à un processus de fermentation. Plus précisément, l'invention concerne une solution saccharifiée ayant pour matière de départ une biomasse comprenant une cellulose. La conductivité électrique de cette solution saccharifiée est supérieure ou égale à ５００μＳ／ｃｍ. La teneur en oligosaccharide dans cette solution saccharifiée est inférieure ou égale à １０ｐｐｍ, la teneur en furfural y est inférieure ou égale à １ｐｐｍ, et la teneur en lignine soluble y est supérieure ou égale à １００ｐｐｍ.
PCT/JP2020/006443 2020-02-19 2020-02-19 Solution saccharifiée WO2021166102A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PCT/JP2020/006443 WO2021166102A1 (fr) 2020-02-19 2020-02-19 Solution saccharifiée
PCT/JP2020/013246 WO2021166273A1 (fr) 2020-02-19 2020-03-25 Solution saccharifiée
PCT/JP2021/006346 WO2021167064A1 (fr) 2020-02-19 2021-02-19 Solution saccharifiée, et solution saccharifiée purifiée
TW110105795A TW202144581A (zh) 2020-02-19 2021-02-19 糖化液及精製糖化液

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PCT/JP2020/006443 WO2021166102A1 (fr) 2020-02-19 2020-02-19 Solution saccharifiée

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Citations (3)

* Cited by examiner, † Cited by third party
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JP2006149343A (ja) * 2004-11-26 2006-06-15 Daitoo Fujitekku:Kk 木質系バイオマスからのグルコース生成物とグルコース生成物製造方法
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