WO2021147092A1

WO2021147092A1 - Grease composition, grease for feed, preparation method therefor and application thereof

Info

Publication number: WO2021147092A1
Application number: PCT/CN2020/074009
Authority: WO
Inventors: 赖州文; 龙吉云; 章亮
Original assignee: 徐州新奥生物科技有限公司
Priority date: 2020-01-23
Filing date: 2020-01-23
Publication date: 2021-07-29

Abstract

A grease composition, comprising bile acid, a bile acid derivative, a bile acid salt or a mixture thereof, and grease. The grease is granulated by the bile acid, bile acid derivative, and bile acid salt, or a mixture chyle thereof. Also provided are a preparation method for said grease composition, a grease for feed comprising a grease composition, and an application of a grease composition or grease for feed in animal rearing.

Description

Oil composition, feed fat and preparation method and application thereof

Technical field

The present application generally relates to the field of animal feed products; more specifically, the present application relates to fat compositions suitable for animal feeding, feed fats, and preparation methods and applications thereof.

Background technique

In animal feed, oil is an indispensable part. Feed fats can provide energy and essential fatty acids for animals. Animals lacking essential fatty acids may cause undesirable results such as growth arrest, decreased reproductive function, subcutaneous edema and bleeding, and body color dullness. Feed oil is also used as a carrier for fat-soluble vitamins A, D, carotenoids, etc., to promote the absorption and utilization of vitamins as fat-soluble substances. The omega 3 fatty acids in feed oil can reduce prostaglandins, thereby increasing the level of antibody production in the carcass, saving the amount of antibiotics for livestock, and achieving the effect of disease prevention and treatment. Emulsifiers are added to the current production process of feed fats to create a hydrophilic environment for the fats to improve the digestion, absorption and utilization rate of the fats.

Because of its biological benefits, bile acids are now used as additives in the production of animal feed. Studies have found that bile acids can emulsify fat in the animal body and expand its contact area with lipase; regulate the activities of pancreatic lipase and lipoprotein esterase to improve the hydrolysis and metabolism of fat; bile acids transport fat in the intestine , Promote the absorption of fat. In addition, bile acids can significantly reduce the catabolism of autologous fat by regulating the activity of hormone-sensitive lipase. Bile acids can save energy raw materials, increase energy utilization, improve growth performance and slaughter performance, and are the "positive energy" for saving resources.

Improved feed fats, especially improved feed fats containing bile acids, bile acid derivatives or bile salts, are needed in the art.

Summary of the invention

In the first aspect, the present application provides a fat composition comprising bile acid, bile acid derivative, bile acid salt or a mixture thereof, and fat, wherein the fat is modified by the bile acid, bile acid derivative, bile acid salt Or its mixture is milky and micronized.

In some embodiments, the bile acid derivative is selected from the group consisting of deoxybile acid, chenodeoxy bile acid, ursodeoxy bile acid, hyodeoxy bile acid, and any combination thereof.

In some embodiments, the bile acid salt is selected from sodium salt, potassium salt, calcium salt, magnesium salt, and any combination thereof.

In some embodiments, the oil is selected from fish oil, linseed oil, seaweed oil, palm oil, coconut oil, rice oil, soybean oil, medium chain triglycerides, and any combination thereof.

In some embodiments, the oil is rich in omega-3 fatty acids.

In some embodiments, the diameter of the formed milkweed microparticles (particulate fats and oils of milkweed) is 10 μm or less.

In the second aspect, the present application provides a method for preparing an oil composition, which comprises emulsifying and homogenizing bile acid, bile acid derivative, bile acid salt or mixture thereof with emulsifier, microcapsule packaging material and oil.

In some embodiments, the method further includes spray drying the homogenized product.

In some embodiments, the bile acid derivative is selected from deoxybile acid, chenodeoxybile acid, ursodeoxybile acid, hyodeoxybile acid, and any combination thereof.

In some embodiments, the emulsifier is selected from the group consisting of sodium stearoyl lactylate, sodium caseinate, mono/diglycerides (e.g., glyceryl mono/distearate), and any combination thereof.

In some embodiments, the microcapsule packaging material is selected from modified starch, dextrin, syrup, chitosan, lactose, whey powder, and any combination thereof.

In some embodiments, the oil is selected from fish oil, linseed oil, seaweed oil, palm oil, coconut oil, rice oil, soybean oil, medium chain triglycerides and any combination thereof. Preferably, the oil is rich in omega -3 fatty acids.

In some embodiments, based on the total weight of the fat composition, the bile acid, bile acid derivative, bile acid salt, or mixture thereof is 0.1 wt.% to 20 wt.%.

In some embodiments, based on the total weight of the fat composition, the fat is 5 wt.% to 80 wt.%.

In some embodiments, based on the total weight of the fat composition, the emulsifier is 0.4 wt.% to 13 wt.%.

In some embodiments, based on the total weight of the oil composition, the microcapsule packaging material is 0.1 wt.% to 90 wt.%.

In some embodiments, the method includes the following steps:

Dissolve bile acid, bile acid derivatives, bile acid salts or mixtures thereof in a weakly alkaline aqueous solution (for example, use alkaline sodium salt or sodium hydroxide to adjust the pH to 8-12), and encapsulate a part of the emulsifier and microcapsules The materials are mixed and dissolved separately or mixed and dissolved in water, and the above aqueous solutions are mixed again to obtain the water phase;

Dissolve part of the emulsifier in the oil to obtain the oil phase;

Mix, emulsify, and homogenize the water phase and the oil phase; and

Optionally, spray drying is performed on the homogenized product.

In some embodiments, the grease composition prepared according to the method described in the second aspect has the properties of the grease composition described in the first aspect.

In the third aspect, the present application provides feed fats and oils, which comprise the fat composition of the first aspect or the fat composition prepared according to the method of the second aspect.

In a fourth aspect, this application provides the oil composition of the first aspect, or the oil composition prepared according to the method of the second aspect, or the use of the feed fat of the third aspect in animal feeding.

Brief description of the drawings

Figures 1 to 4 show a transmission electron microscope comparison of a bile acid-free grease composition prepared according to conventional techniques (above picture) and a grease composition according to the method of the present application (below picture), wherein the magnifications of Figs. 1 to 4 Respectively 2500, 10000, 25000, 50000 times.

Detailed description of the invention

The inventors of the present application conducted research on the preparation of feed fats containing bile acid, bile acid derivatives or bile acid salts. Bile acids can emulsify fat in the animal body to form chylous microparticles. If chylous microparticles can be achieved in the production process of feed fats (in vitro), the intestinal mucosal epithelial cells can better recognize them. Grease quickly transports and transfers oil into the body with affinity. As an exogenous bioemulsifier, bile acid can activate lipase and improve the digestion, absorption and utilization rate of fat, in addition to transporting fat as particles. In contrast, conventional chemical emulsifiers can only play a role in creating a hydrophilic environment for oils and improving the digestion and absorption of oils. In addition, although bile acids have many biological benefits, bile acids, bile acid derivatives, or bile acid salts usually have a bitter taste, and adding them directly to feed or feed fats can cause palatability problems. In view of the above technical problems, the inventors of this application have established various inventions of this application through research. The various inventions of this application can solve at least one of the above technical problems, and in some cases also have other advantages.

definition

The following definitions are provided to better define this application and to guide those of ordinary skill in the art in the practice of this application. Unless otherwise specified, the meanings of terms in this application are the same as those commonly understood by those skilled in the art, for example, terms related to raw materials and products, operating steps, process parameters, equipment and tools used, and numerical units. All the patent documents, academic papers and other public publications cited in this article are incorporated herein by reference in their entirety.

The term "bile acid derivative" as used herein refers to a substance suitable for administration to animals whose structure is derived from bile acid. For example, common bile acid derivatives are deoxy bile acids, such as chenodeoxy bile acid, ursodeoxy bile acid, hyursodeoxy bile acid, and the like.

The term "bile salt" as used herein refers to a salt of bile acid suitable for administration to animals. For example, suitable bile acid salts include sodium, potassium, calcium, magnesium, and the like.

The term "fat" as used herein refers to oils and fats suitable as animal feed. For example, suitable oils include, but are not limited to, fish oil, linseed oil, seaweed oil, palm oil, coconut oil, rice oil, soybean oil, medium chain triglycerides, and the like.

The term "emulsifier" used herein is in accordance with the meaning understood by those skilled in the field of feed fat production. The addition of emulsifiers in the production process can create a hydrophilic environment for the fats and thereby improve the digestion and absorption utilization of the fats. For example, suitable emulsifiers include, but are not limited to, sodium stearoyl lactylate, sodium caseinate, mono/diglycerides (such as glyceryl mono/distearate) and the like.

As used herein, the term "microcapsule packaging material", also called "microcapsule wall material" has the meaning understood by those skilled in the field of feed fat production. Generally, in the microencapsulation process, the microcapsule packaging material is a part of the water phase, and the water phase and the oil phase are homogenized by grinding or shearing, and then spray-dried to form microcapsules. For example, suitable microcapsule packaging materials include, but are not limited to, modified starch, dextrin, syrup, chitosan, lactose, whey powder and the like.

Specific implementation plan

In some embodiments, bile acid derivatives such as deoxybile acid, chenodeoxy bile acid, ursodeoxy bile acid, and any combination thereof.

In some embodiments, the oil is rich in omega-3 fatty acids.

In some embodiments, the method further includes spray drying the emulsified homogenized product.

In some embodiments, based on the total weight of the fat composition, the bile acid, bile acid derivative, bile acid salt, or mixture thereof is 0.1 wt.% to 20 wt.%, for example, 0.1 wt.%, 0.5 wt.% , 1wt.%, 2wt.%, 3wt.%, 4wt.%, 5wt.%, 6wt.%, 7wt.%, 8wt.%, 9wt.%, 10wt.%, 11wt.%, 12wt.%, 13wt %, 14wt.%, 15wt.%, 16wt.%, 17wt.%, 18wt.%, 19wt.%, 20wt.%, or a range composed of any two of the above values. In some embodiments, based on the total weight of the fat composition, the bile acid, bile acid derivative, bile acid salt, or mixture thereof is 1 wt.% to 10 wt.%. In some embodiments, based on the total weight of the fat composition, the bile acid, bile acid derivative, bile acid salt, or mixture thereof is 1 wt.% to 5 wt.%.

In some embodiments, based on the total weight of the fat composition, the fat is 5wt.% to 80wt.%, for example, 5wt.%, 10wt.%, 15wt.%, 20wt.%, 25wt.%, 30wt.% , 35wt.%, 40wt.%, 45wt.%, 50wt.%, 55wt.%, 60wt.%, 65wt.%, 70wt.%, 75wt.%, 80wt.%, or a range of any two of the above values . In some embodiments, based on the total weight of the fat composition, the fat is 10 wt.% to 50 wt.%. In some embodiments, based on the total weight of the grease composition, the fat is 30 wt.% to 50 wt.%.

In some embodiments, based on the total weight of the fat composition, the emulsifier is 0.4wt.%-13wt.%, for example, 0.4wt.%, 0.6wt.%, 0.8wt.%, 1wt.%, 2wt. %, 3wt.%, 4wt.%, 5wt.%, 6wt.%, 7wt.%, 8wt.%, 9wt.%, 10wt.%, 11wt.%, 12wt.%, 13wt.%, or any two of the above A range of values.

In some embodiments, based on the total weight of the fat composition, the microcapsule packaging material is 0.1wt.% to 90wt.%, for example, 0.1wt.%, 0.5wt.%, 1wt.%, 5wt.%, 10wt. %, 15wt.%, 20wt.%, 25wt.%, 30wt.%, 35wt.%, 40wt.%, 45wt.%, 50wt.%, 55wt.%, 60wt.%, 65wt.%, 70wt.%, 75wt.%, 80wt.%, 85wt.%, 90wt.%, or a range composed of any two of the above values.

In some embodiments, the method includes the following steps:

(a) Dissolve bile acid, bile acid derivatives, bile acid salts, or mixtures thereof in a weakly alkaline aqueous solution (for example, use alkaline sodium salt or sodium hydroxide to adjust the pH to 8-12), add a part of the emulsifier and The microcapsule packaging materials are mixed and dissolved separately or mixed and dissolved in water, and the above aqueous solutions are remixed to obtain the water phase;

(b) Dissolve a part of the emulsifier in the fat to obtain the oil phase;

(c) Mixing, emulsifying and homogenizing the water phase and the oil phase; and

(d) Optionally, spray drying the homogenized product.

In some embodiments, the grease in step (b) is heated to 50°C to 90°C, for example, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C .

In some embodiments, the emulsification treatment is colloidal grinding or shear pump shearing.

In some embodiments, the homogenization treatment is performed under high pressure, such as 20-45 MPa, for example, 20 MPa, 25 MPa, 30 MPa, 35 MPa, 40 MPa, 45 MPa.

In some embodiments, spray drying is performed under high pressure, such as 2-20 MPa, such as 2 MPa, 5 MPa, 10 MPa, 15 MPa, 20 MPa.

In some embodiments, the inlet air temperature of spray drying is controlled at 135-210°C (for example, 135°C, 140°C, 150°C, 160°C, 170°C, 180°C, 190°C, 200°C, 210°C). The wind temperature is controlled to 55-105°C (for example, 55°C, 65°C, 75°C, 85°C, 95°C, 105°C).

As a non-limiting example, the preparation method of the grease composition can be implemented as follows:

Dissolve bile acid, bile acid derivatives, bile acid salts or their mixture in weakly alkaline water according to the formula ratio (use alkaline sodium salt or sodium hydroxide to adjust the pH to 8-12), sodium caseinate, and modified starch Dissolve in water, other microcapsule packaging materials (dextrin, syrup, chitosan, lactose, whey powder) are dissolved in water, and the two are mixed and stirred evenly; the mono/diglycerides (such as mono/di stearic acid) Glycerides) and sodium stearoyl lactylate are dissolved in the oil phase heated to 50-90°C. The water phase and the oil phase are mixed and ground by a colloid mill or sheared by a high-shear pump. The homogenization pressure is controlled to 20-45MPa to homogenize the material liquid at high pressure, and the homogenized emulsion is filled into bile acid emulsified oil. If filling is not carried out, the material can be pressurized to 2-20MPa by a high-pressure pump for pressure spray drying. Control the inlet air temperature of the drying tower to 135-210°C and the outlet temperature of 55-105°C to spray dry the material to obtain water-soluble bile acid emulsified oil microcapsule powder.

The microencapsulated fat prepared according to the method of the present application, because the wall material of the microcapsule usually contains sweet syrup or dextrin and other substances, through the process of the present application, the bile acid is bounded between the oil phase and the water phase syrup. The sweetness of the material can conceal the bitterness of bile acid to a certain extent and help solve the problem of palatability.

In addition, the microencapsulated oil prepared according to the method of the present application can realize the milk micronization of the oil in vitro, which enables the intestinal mucosal epithelial cells to better recognize the oil and affinity to quickly transport and transport the oil into the body. As an exogenous biological emulsifier, bile acid can activate lipase and improve the digestion, absorption and utilization rate of oil, in addition to transporting oil as particles.

Animals suitable for administering the fat composition or feed fat of the present application include but are not limited to livestock and poultry, fish and the like. Animals suitable for administering the feed composition of the present application are not particularly limited, and suitable animals may be, for example, livestock, poultry, or aquatic products. The "livestock" in this article refers to mammals in the breeding industry, such as pigs, cattle, sheep, dogs, cats, rabbits, etc. The "poultry" in this article refers to poultry animals in the breeding industry, such as chickens, ducks, geese, and bird breeding animals. "Aquatic products" in this article refers to aquatic animals in the aquaculture industry, such as fish, shrimp, crabs, and bullfrogs.

The fat composition or feed fat of the present application can be directly added to water for animals to drink or directly mixed with feed and eaten.

The beneficial effects that can be achieved by using the oil composition of the present application for feeding animals include, but are not limited to, improving the laying quality of laying hens (for example, increasing the DHA content of eggs), and increasing the newborn weight, weaning weight, and daily weight gain of newborn mammals. Or weaning survival rate to promote fish growth.

Example

The inventions of the present application are exemplarily described below in conjunction with the embodiments, but the content of the embodiments does not limit the inventions of the present application in any way.

Example 1

Exemplary raw material formulations for preparing the grease composition of the present application are shown in Table 1.

Table 1

The preparation process is as follows:

Dissolve bile acid, bile acid derivatives, bile acid salts or their mixture in weakly alkaline water according to the formula ratio (use alkaline sodium salt or sodium hydroxide to adjust the pH to 8-12), sodium caseinate, and modified starch Dissolve in water, other microcapsule packaging materials (dextrin, syrup, chitosan, lactose, whey powder) are dissolved in water, and the two are mixed and stirred evenly; the mono/diglycerides (such as mono/di stearic acid) Glycerides) and sodium stearoyl lactylate are dissolved in the oil phase heated to 50-90°C. The water phase and the oil phase are mixed, and ground by a colloid mill or sheared by a high-shear pump, and the homogenization pressure is controlled to 20-45MPa to perform high-pressure homogenization of the material liquid, and the homogenized emulsion is filled into bile acid emulsified oil. If filling is not carried out, the material can be pressurized by a high-pressure pump to 2-20MPa for pressure spray drying. Control the inlet air temperature of the drying tower to 135-210°C and the outlet temperature of 55-105°C to spray dry the material to obtain water-soluble bile acid emulsified oil microcapsule powder.

Example 2

The fat composition prepared in Example 1 was added to grass carp feed and broiler feed at a ratio of 3 kg/t, and fed to grass carp and broiler chickens for four weeks, respectively. The serum triglycerides, intestinal compact protein, malondialdehyde, Liver glutathione peroxidase (GSH-PX) and superoxidase (SOD) are used as indicative indicators for detection.

As a control, feed simply mixed with bile acids (for example, 2.91 kg of ordinary feed + 90 grams of bile acid) was added to grass carp feed and broiler feed at 3 kg/t, and the feeding process and detection indicators were the same as above.

Comparing the test results of the above two groups, it is found that compared with the control, the comprehensive indexes of grass carp and broiler chickens administered with the oil composition of the present application are improved by more than 5%.

Example 3

The oil and fat composition prepared in Example 1 was subjected to transmission film analysis with a control oil and fat composition prepared according to a conventional process similar to that in Example 1, but without bile acid components. As shown in Figures 1-4, in the oil composition prepared according to the method of the present application, the hydrophilic and lipophilic groups of bile acid are combined between water and oil. The emulsified and dispersed emulsified oil particles are further dispersed into smaller chylomicrons; while the control oil composition does not have such properties.

Example 4. Egg laying quality test of laying hens

The raw material formulation of the grease composition tested in this example is shown in Table 2, and the preparation process refers to Example 1.

Table 2

胆汁酸盐Bile acid salt	3％3%
深海鱼油Deep sea fish oil	50％50%
单双硬酯酸甘油酯Glyceryl monostearate	2.5％2.5%
硬酯酰乳酸钠Sodium stearoyl lactylate	1.5％1.5%
酪朊酸钠Sodium Caseinate	3％3%
变性淀粉Modified starch	5％5%
糊精dextrin	35％35%

Test site: Zhiyu Farm, Yuanyang County, Xinxiang City, Henan Province

Test grouping:

A: Control group (conventional feed for laying hens)

B: Test group (conventional feed for laying hens + 5% oil composition of this embodiment)

Each group of 200 57-week-old "Nongda No. 3" white feather laying hens were fed continuously for 30 days. The results are shown in Table 3 below:

Table 3. DHA content in eggs

To

A

B

DHA content of 100g eggs (g)

Below detection limit

0.2415

As shown in the results in Table 3, adding the oil composition of the present application to the diet of laying hens can increase the DHA content of eggs.

Example 5. Testing of newborn weight, weaning weight and weaning survival rate of suckling piglets

The raw material formulation of the grease composition tested in this example is shown in Table 4, and the preparation process refers to Example 1.

Table 4

胆汁酸 bile acid	2％2%
亚麻籽油Linseed oil	50％50%
单双硬脂酸甘油酯Glyceryl monostearate	1.5％1.5%
硬酯酰乳酸钠Sodium stearoyl lactylate	1.2％1.2%

酪朊酸钠Sodium Caseinate	2％2%
变性淀粉Modified starch	3％3%
糖浆syrup	40.3％40.3%

Test site: Xinfa Farm, Xihua County, Zhoukou City, Henan Province

Test grouping:

A: Control group (Regular feed for lactating sows)

B: Test group (Regular feed for lactating sows + 2% oil composition of this embodiment)

Each group of 12 lactating sows with similar parities and body conditions were weaned from the 91st day of gestation to 22 days after the suckling piglets. The results are shown in Table 5 below:

table 5

As shown in the results in Table 5, adding the lipid composition of the present application to the diet of perinatal and lactating sows can increase the newborn weight of suckling piglets, the average weaning rate of suckling piglets, and the survival rate of suckling piglets from weaning.

Example 6. Testing of newborn weight, weaning weight and weaning survival rate of suckling piglets

The raw material composition of the grease composition tested in this example is shown in Table 6, and the preparation process refers to Example 1.

Table 6

胆汁酸 bile acid	2％2%
椰子油coconut oil	50％50%
单双硬脂酸甘油酯Glyceryl monostearate	1.5％1.5%
硬酯酰乳酸钠Sodium stearoyl lactylate	1.2％1.2%

酪朊酸钠Sodium Caseinate	2％2%
变性淀粉Modified starch	3％3%
糖浆syrup	40.3％40.3%

Test location: Sir Jinxu Pig Farm

Test grouping:

A: Control group (Regular feed for lactating sows)

B: Test group (Regular feed for lactating sows + 2% of the oil composition of the present embodiment)

Each group of 10 lactating sows with similar parities and body conditions were weaned from 5 days before delivery to 22 days after suckling piglets. The results are shown in Table 7 below:

Table 7

As shown in the results in Table 5, adding the oil composition of the present application to the diets of perinatal and lactating sows can increase the average weaning weight of suckling piglets, the daily increase of suckling piglets, and the survival rate of suckling piglets from weaning.

Example 6. Growth of sea bass, liver function and serum biochemical index test

The raw material composition of the grease composition tested in this example is shown in Table 8, and the preparation process refers to Example 1.

Table 8

胆汁酸盐Bile acid salt	3％3%
深海鱼油Deep sea fish oil	30％30%
单双硬脂酸甘油酯Glyceryl monostearate	3％3%
硬酯酰乳酸钠Sodium stearoyl lactylate	1.6％1.6%

酪朊酸钠Sodium Caseinate	2％2%
变性淀粉Modified starch	10％10%
糊精dextrin	50.4％50.4%

Test location: Jimei Fisheries College

Test grouping:

A: Test 1 group (self-prepared sea bass diet + 0.5% compound 1)

B: Test 2 group (self-prepared seabass diet + 0.5% compound 2)

C: Test 3 groups (self-prepared seabass diet + 0.5% oil composition of this embodiment)

Note: Compound 2 is a mixture that has exactly the same components as the oil and fat composition of this example but has not been homogenized and emulsified. Complex 1 is complex 2 without bile salts.

Each group has 4 repetitions, each repetition is one tank, and each tank has 20 seabass. Feed 3 times a day at regular intervals, and the experimental period is 8 weeks. The results are shown in Table 9 below:

Table 9

Note: The different letters in the shoulder of the peer data indicate significant differences (p<0.05).

As shown in the results in Table 9, adding 0.5% of the fat composition of the present application to the diet of sea bass has the following beneficial effects:

Significantly increase the weight gain rate and feed intake of sea bass;

Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were significantly reduced, indicating that the burden on the liver was reduced;

Alkaline phosphatase increased significantly, indicating that it is beneficial to improve the utilization of nutrients

The lipoprotein lipase (LPL) is significantly increased, and the triglyceride (TG) content in the liver is significantly reduced, indicating that the oil composition of the present application can increase the activity of lipolytic enzymes and reduce the liver triglyceride content.

In the above, the present invention has been described in detail with general descriptions and specific embodiments, but on the basis of the present invention, some modifications or improvements can be made, which is obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all belong to the scope of the present invention.

Claims

An oil and fat composition comprising bile acid, bile acid derivative, bile acid salt or a mixture thereof, and oil, wherein the oil is micronized by the bile acid, bile acid derivative, bile acid salt or the mixture thereof.
The oil composition of claim 1, wherein

The bile acid derivatives are selected from deoxybile acid, chenodeoxybile acid, ursodeoxybile acid, hyodeoxybile acid and any combination thereof; and/or

The bile acid salt is selected from sodium salt, potassium salt, calcium salt, magnesium salt and any combination thereof.
The fat composition according to any one of claims 1-2, wherein the fat is selected from fish oil, linseed oil, seaweed oil, palm oil, coconut oil, rice oil, soybean oil, medium chain triglycerides, And any combination thereof, preferably, the oil is rich in omega-3 fatty acids.
The oil and fat composition according to any one of claims 1 to 3, wherein the diameter of the formed milky particles is 10 μm or less.
The method for preparing a fat composition includes the following steps:

Emulsify and homogenize bile acids, bile acid derivatives, bile salts or their mixtures with emulsifiers, microcapsule packaging materials and oils; and

Optionally, spray drying is performed on the homogenized product.
The method of claim 5, wherein

The bile acid derivative is selected from deoxybile acid, chenodeoxybile acid, ursodeoxybile acid and any combination thereof; and/or

The bile acid salt is selected from sodium salt, potassium salt, calcium salt, magnesium salt and any combination thereof; and/or

The emulsifier is selected from the group consisting of sodium stearoyl lactylate, sodium caseinate, mono/diglycerides (such as glyceryl mono/distearate), and any combination thereof; and/or

The microcapsule packaging material is selected from modified starch, dextrin, syrup, chitosan, lactose, whey powder and any combination thereof; and/or

The fat is selected from fish oil, linseed oil, seaweed oil, palm oil, coconut oil, rice oil, soybean oil, medium chain triglycerides, and any combination thereof. Preferably, the fat is rich in omega-3 fatty acids.
The method according to claim 5 or 6, wherein based on the total weight of the fat composition,

The bile acid, bile acid derivative, bile acid salt or mixture thereof is 0.1 wt.% to 20 wt.%, preferably 1 wt.% to 10 wt.%, more preferably 1 wt.% to 5 wt.%; and/or

The fat is 5wt.%～80wt.%, preferably 10wt.%～50wt.%, more preferably 30wt.%～50wt.%; and/or

The emulsifier is 0.4wt.% to 13wt.%; and/or

The microcapsule packaging material is 0.1wt.% to 90wt.%.
The method of any one of claims 5 to 7, comprising the following steps:

Dissolve bile acid, bile acid derivatives, bile acid salts or mixtures thereof in a weakly alkaline aqueous solution, mix a part of the emulsifier and microcapsule packaging materials to dissolve or mix and dissolve separately in water, and then mix the above aqueous solutions to obtain a water phase ；

Dissolve part of the emulsifier in the oil to obtain the oil phase;

Mix, emulsify, and homogenize the water phase and the oil phase; and

Optionally, spray drying is performed on the homogenized product.
Feed fats and oils comprising the fat composition according to any one of claims 1 to 4 or the fat composition prepared according to the method according to any one of claims 5-8.
The fat composition of any one of claims 1 to 4, or the fat composition prepared according to the method of any one of claims 5-8, or the feed fat of claim 9 in animal breeding In the use.