WO2021133006A1 - Composition pour réduire les rides de la peau ou prévenir le vieillissement de la peau, contenant un extrait de radis séché et torréfié et aliments naturels fonctionnels comprenant la composition - Google Patents

Composition pour réduire les rides de la peau ou prévenir le vieillissement de la peau, contenant un extrait de radis séché et torréfié et aliments naturels fonctionnels comprenant la composition Download PDF

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Publication number
WO2021133006A1
WO2021133006A1 PCT/KR2020/018741 KR2020018741W WO2021133006A1 WO 2021133006 A1 WO2021133006 A1 WO 2021133006A1 KR 2020018741 W KR2020018741 W KR 2020018741W WO 2021133006 A1 WO2021133006 A1 WO 2021133006A1
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WIPO (PCT)
Prior art keywords
radish
skin
extract
composition
dried
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PCT/KR2020/018741
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English (en)
Korean (ko)
Inventor
김희진
이기범
정지형
신승철
Original Assignee
제주돌담뜰 농업회사법인(주)
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Priority claimed from KR1020200016279A external-priority patent/KR102437871B1/ko
Application filed by 제주돌담뜰 농업회사법인(주) filed Critical 제주돌담뜰 농업회사법인(주)
Publication of WO2021133006A1 publication Critical patent/WO2021133006A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products

Definitions

  • endogenous aging is an unavoidable aging phenomenon with the passage of time, and its clinical characteristics are relatively mild, and include fine wrinkles, dry skin, and decreased elasticity.
  • the present invention in consideration of the prior art described above, not only enhances the antioxidant effect of dried radish by roasting and processing dried radish, but also improves skin wrinkles or prevents skin aging without irritation to the human body, and a composition thereof To provide health functional food containing
  • the roasted radish extract of the composition for skin wrinkle improvement or skin aging prevention according to the present invention is an extract extracted by roasting dried radish radish to a moisture content of 3% by weight at 200° C. for 30 minutes.
  • the health functional food according to the present invention is characterized in that it comprises the above-described composition for improving skin wrinkles or preventing skin aging and drinking water (water).
  • composition containing the roasted radish extract according to the present invention as an active ingredient is effective in improving skin wrinkles or preventing skin aging.
  • 1 is a representative image of the back skin of a mouse for observing skin redness and keratinization according to the lapse of time in the skin application test group.
  • 3 is a representative image of the back skin of a mouse for observing skin redness and keratinization over the course of the oral administration experimental group.
  • Figure 4 is a graph measuring the thickness of the skin thickness according to the period of the oral administration experimental group.
  • 5 is a graph measuring skin hydration (skin moisture) according to the lapse of the period of the oral administration experimental group.
  • FIG. 7 is a histological image of the skin of a mouse, etc. for histopathological observation according to the lapse of the period of the skin application test group.
  • FIG. 8 is a graph showing the thickness of the epidermis and the thickness of the dermis according to the period of the skin application test group.
  • 9 is a histological image of the skin of a mouse, etc. for histopathological observation according to the period of the oral administration experimental group.
  • 10 is a graph measuring the thickness of the epidermis and the thickness of the dermis according to the period of the oral administration experimental group.
  • 11 is a graph measuring changes in the concentrations of type I collagen, type III collagen, catalase, GPX and SOD according to the lapse of time in the skin application test group.
  • 13 is a graph measuring the change in the concentration of TNF- ⁇ .
  • composition for improving skin wrinkles or preventing skin aging is characterized in that it contains the roasted radish extract as an active ingredient.
  • the roasted dried radish extract is preferably an extract of dried radish dried radish at a temperature of 180 to 200 ° C. for 15 to 30 minutes, and dried radish dried to a moisture content of 3% by weight at 200 ° C. for 30 minutes.
  • An extract obtained by hot water extraction of one radish is more preferable.
  • composition for improving skin wrinkles or preventing skin aging according to the present invention may be for oral administration for skin application.
  • the health functional food according to the present invention is characterized in that it comprises the above-described composition for improving skin wrinkles or preventing skin aging and drinking water (water).
  • Radish (Raphanus sativus L.) was purchased from Jeju Agricultural Products Market on December 2018 and used as a sample. After removing the soil and fine roots of the purchased radish, washed clean, cut the skin into 1.2 ⁇ 4.5 ⁇ 0.6 cm, put it in a hot air dryer, and dried the hot air at 55 ° C. for 24 hours to prepare dried radish. The moisture content of the dried radish was measured to be 3% by weight based on the total weight of the dried radish.
  • Example 1.1 500 g of dried radish prepared in Example 1.1 were subjected to a stir-frying device, including moisture content (3 wt%, 5.9 wt%, 26 wt%, 27 wt%), roasting temperature (180, 200°C), And the roasting time (15 to 30 minutes) was varied, and the roasted radish dried radish was cooled at room temperature and then stored refrigerated until use.
  • the dried radish was dried at 180 to In the dried radish extract (Samples 1 to 9) roasted at 200° C. for 15 to 30 minutes, the total polyphenol content in the extract was significantly higher.
  • dried radish with a moisture content of 3 wt % was roasted at 200° C. for 30 minutes.
  • the electron donating ability to DPPH was measured by the Bondet method, and after adding 2 ⁇ l of each of the 10 extract samples according to ⁇ Table 1> to 198 ⁇ l of 300 ⁇ M DPPH/EtOH, it was left at 37° C. for 30 minutes and then UV/ Absorbance was measured at 517 nm using a visible spectrophotometer.
  • the electron donating ability of each sample was obtained in % by the following formula.
  • the dried radish extract (Sample 10) having a moisture content of 3% by weight was simply dried at a temperature of 55° C. in warm air and not subjected to stir-frying treatment
  • the dried radish was dried at 180 to 180 to
  • the IC 50 value was found to be significantly lower in the radish extract (Samples 1 to 9) roasted at 200° C. for 15 to 30 minutes.
  • the antioxidant efficacy of the radish is significantly increased when the dried radish is roasted at a temperature of 180 to 200° C. for 15 to 30 minutes.
  • the reducing sugar content of the dried radish extract was measured to be 325 mg/g, respectively, and the reducing sugar content of the roasted radish extract was measured to be 310 mg/g. In other words, it was found that reducing sugar was reduced by about 5% in the process of roasting dried radish dried radish with roasted radish.
  • the concentration was calculated from the area by comparing the peak obtained by injecting 20 ⁇ l of the sample into the detector (HP1100, UV280 nm) with the peak obtained from the standard 5-HMF (Sigma-Aldrich Co., USA), and the result is as follows. It is shown in [Table 5].
  • 5-HMF was not detected in dried radish, but 5-HMF was measured to be 7.44 mg/100g in roasted radish.
  • the Maillard reaction is a reaction that occurs when sugars and amino acids are condensed (amino-carbonyl reaction), and free amino acids and reducing sugars participate in the reaction.
  • the color of the roasted radish was dark brown and showed a significant difference in chromaticity from that of the dried radish, which is thought to be due to the formation of melanoidin pigment by the Maillard reaction during the roasting process.
  • the Maillard reaction product has strong antioxidant activity, and in particular, it has been reported that 5-HMF, an intermediate product, has physiological activities such as inhibition of nitric oxide production and inhibition of tyrosinase. It can be seen that antioxidants are produced by Maillard reaction during the roasting process of dried radish radish.
  • Example 4 Efficacy of skin dermal cell proliferation and SOD (Superoxide dismutase)-like activity
  • Human dermal fibroblasts (PromoCell, USA) were cultured in an incubator at 37°C, 5% CO 2 , and Dulbecco's modified Eagal medium (FBS) containing 100 units/ml penicilin-streptomycin and 5% fetal bovine serum (FBS) as a culture medium DMEM) was used. Cells are cultured while changing the culture medium 2-3 times a week, and when cell differentiation reaches the maximum on day 6-7, wash the cells with phosphate buffered saline (PBS) and then trypsion-EDTA solution (0.05% trypsin and 0.02% EDTA).
  • PBS phosphate buffered saline
  • centrifugation was performed to collect the cells, put them in a medium, mixed them well with a pipette to distribute the cells evenly, and dispensed them in several barrels, and then they were used for subculture while stored in liquid nitrogen.
  • Cells used in the experiment were subcultured in the same manner as above, and each passage number was recorded during culture. When the passage number was 10 or more, new cells were taken out from the liquid nitrogen tank and cultured again to be used as experimental cells.
  • the human skin dermis cells human dermal fibroblasts (HDFs, PromoCell, USA) a 96-well plate in (5x10 3 cells / well) seeding with 5x10 3 cells / well and in DMEM (10% FBS, 1% penicillin streptomycin) medium
  • DMEM 50% FBS, 1% penicillin streptomycin
  • the dried radish extract treated group showed 89% and 87% cell viability effects at 300 and 500 ⁇ g/ml, respectively, and in the case of the stir-fried radish extract treated group, 95% and 110% at 300 and 500 ⁇ g/ml, respectively. of the cell viability effect, the cell viability increased by about 6% and 23% in the case of the roasted dried radish extract treatment group compared to the dried radish extract treatment group.
  • HDFs Human dermal fibroblasts
  • PromoCell which are human skin dermal cells
  • SOD-like activity was measured using a superoxide dismutase activity kit (Cayman, USA), as shown in [Table 7] below.
  • both the dried radish extract and the stir-fried radish extract increased the SOD-like activity in a concentration-dependent manner.
  • the relative SOD-like activity of 1.24 and 1.25 at 300 and 500 ⁇ g/ml, respectively were shown, while in the roasted dried radish-treated group, the relative SOD-like activity was 1.27 and 1.28 at 300 and 500 ⁇ g/ml, respectively. SOD-like activity was shown. That is, it was found that the relative SOD-like activity effect increased by about 2.4% and 2.4% in the case of the roasted dried radish-treated group compared to the dried radish-treated group.
  • Species and strains of experimental animals were purchased from Orient Bio (address: 322, Galmachi-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do) as female SKH-1 hairless specific pathogen-free (SPF) mice.
  • experimental animals were housed in a polycarbonate breeding box (240W ⁇ 390L ⁇ 175H mm) for acclimatization, quarantine and administration, and observation periods, each of 6 mice or less, and identification of individuals was performed by labeling using an ear punch and by breeding box.
  • the individual identification card display method was used.
  • anesthesia was induced by setting the oxygen flowmeter to 0.9L/min and setting the isoflurane concentration to 3.5%.
  • the oxygen flowmeter was maintained at 4 ⁇ 0.8L/min using a mask, and the anesthesia was maintained by setting the isoflurane concentration to 1.5%.
  • the core body temperature was maintained at 37-38°C using a warming mat.
  • UV rays were irradiated under animal anesthesia, and the head including eyes was irradiated with UV rays after blocking UV rays.
  • the UV irradiator installs a UV lamp that generates UV rays of 280-320 nm (peak 313 nm) in the cabinet that can be connected to the anesthesia machine, and the intensity of UV rays located at the bottom of the cabinet is measured through a UVmeter to obtain 1 MED (week 1) and 2 Apply at MED (weeks 2-6) intensity. UV irradiation was carried out 3 times a week, 2 hours after application of the test substance, and the distance between the skin of the mouse's back and the UV lamp was 30 cm or more.
  • the UV irradiator installs a UV lamp that generates UV rays of 280-320 nm (peak 313 nm) in the cabinet that can be connected to the anesthesia machine, and the intensity of UV rays located at the bottom of the cabinet is measured through a UVmeter to obtain 1 MED (week 1) and 2 Apply at MED (weeks 2-6) intensity. UV irradiation was carried out 3 times a week, 2 hours after application of the test substance, and the distance between the skin of the back of the mouse and the UV lamp was 30 cm or more.
  • the skin redness symptoms of the UV irradiation test group (VC, RE-TPO, RE-TPOW) are alleviated over time from 3 to 6 weeks, compared to the excipient control group (VC), the test substance applied group ( RE-TPO, RE-TPOW) was observed to have a faster remission rate, and the degree of keratin formation was observed to be less in the test substance application group (RE-TPO, RE-TPOW) than in the excipient control group (VC) ([Fig. 3]).
  • the normal control group (NC) also showed a decrease in the dermal layer thickness over time, but the test substance applied groups (RE-T, RE-TW) showed a normal dermal layer thickness during the entire test period (NC). It was observed to be thicker than the excipient control group (VC).
  • the test substance application group (RE-T) showed a statistically significant (p ⁇ 0.05) increase in the dermal layer thickness compared to the excipient control group (VC) at week 6 (see [Fig. 7] and [Fig. 8]).
  • the epidermal layer was thicker than that of the normal control group (NC) in all of the UV irradiation test groups (VC, RE-TPO, RE-TPOW) during the entire test period.
  • the groups (VC, RE-TPO, RE-TPOW) in particular, in the case of the test group (RE-TPOW), in which the test substance was orally administered with drinking water, the thinnest epidermal layer thickness among the UV irradiation test groups at the 6th week at the end of the test was shown.
  • the thickness of the epidermal layer exhibited by proliferation was similar during the entire test period, but in the case of the test substance application group (RE-T, RE-TW), the thickness of the epidermal layer increased over time from 3 weeks to 6 weeks. was observed to decrease, and the thickness of the epidermal layer was observed to be thinner than that of the excipient control group (VC).
  • test substance application group RE-TPO, RE-TPOW
  • VC excipient control group
  • VC excipient control group
  • the reduction in the thickness of the dermal layer due to photoaging is caused by an increase in inflammatory mediators due to the generation of reactive oxygen species (ROS) by UVB ultraviolet rays, and collagenase (MMPs) secreted by dermal fibroblasts by these inflammatory mediators.
  • ROS reactive oxygen species
  • MMPs collagenase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention concerne une composition pour réduire les rides de la peau ou prévenir le vieillissement de la peau sans provoquer d'irritation sur le corps humain, et des aliments naturels fonctionnels comprenant la composition, la composition comprenant du radis séché et torréfié en tant que principe actif.
PCT/KR2020/018741 2019-12-24 2020-12-21 Composition pour réduire les rides de la peau ou prévenir le vieillissement de la peau, contenant un extrait de radis séché et torréfié et aliments naturels fonctionnels comprenant la composition WO2021133006A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2019-0173951 2019-12-24
KR20190173951 2019-12-24
KR1020200016279A KR102437871B1 (ko) 2019-12-24 2020-02-11 볶음 처리한 무말랭이 추출물을 함유하는 피부주름 개선 또는 피부노화 예방용 조성물 및 그 조성물을 포함하는 건강기능식품
KR10-2020-0016279 2020-02-11

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WO2021133006A1 true WO2021133006A1 (fr) 2021-07-01

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090076219A (ko) * 2008-01-08 2009-07-13 (주)세전 가압 볶음 무말랭이 및 이의 용도
KR20190036330A (ko) * 2017-09-27 2019-04-04 서원대학교산학협력단 열처리된 무 추출물을 포함하는 항산화 조성물
KR20190116837A (ko) * 2018-04-05 2019-10-15 서원대학교산학협력단 무 추출물을 함유하는 피부 주름 및 탄력 개선용 조성물

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090076219A (ko) * 2008-01-08 2009-07-13 (주)세전 가압 볶음 무말랭이 및 이의 용도
KR20190036330A (ko) * 2017-09-27 2019-04-04 서원대학교산학협력단 열처리된 무 추출물을 포함하는 항산화 조성물
KR20190116837A (ko) * 2018-04-05 2019-10-15 서원대학교산학협력단 무 추출물을 함유하는 피부 주름 및 탄력 개선용 조성물

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Dried Radish Tea That is Good for Your Skin", 11 September 2015 (2015-09-11), pages 1 - 8, XP009528736, Retrieved from the Internet <URL:https://blog.naver.com/rltjru/220265720100> *
ANONYMOUS: "Take Care of Your Bronchial Health with Vitamin C-Rich Dried Radish Tea in the Fall", COUNTRY YOUTH, 11 September 2015 (2015-09-11), XP055825042, Retrieved from the Internet <URL:https://blog.daum.net/youngricher/82> *

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