WO2021103528A9 - Procédé de construction de plasmide rapporteur d'activité de promoteur de pdgfb - Google Patents

Procédé de construction de plasmide rapporteur d'activité de promoteur de pdgfb Download PDF

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Publication number
WO2021103528A9
WO2021103528A9 PCT/CN2020/099603 CN2020099603W WO2021103528A9 WO 2021103528 A9 WO2021103528 A9 WO 2021103528A9 CN 2020099603 W CN2020099603 W CN 2020099603W WO 2021103528 A9 WO2021103528 A9 WO 2021103528A9
Authority
WO
WIPO (PCT)
Prior art keywords
constructing
pdgfb
promoter activity
reporter plasmid
activity reporter
Prior art date
Application number
PCT/CN2020/099603
Other languages
English (en)
Chinese (zh)
Other versions
WO2021103528A1 (fr
Inventor
翟旭光
Original Assignee
南通大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 南通大学 filed Critical 南通大学
Publication of WO2021103528A1 publication Critical patent/WO2021103528A1/fr
Publication of WO2021103528A9 publication Critical patent/WO2021103528A9/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/49—Platelet-derived growth factor [PDGF]

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de construction d'un plasmide rapporteur d'activité de promoteur du gène PDGFB, applicable à l'étude de l'activité de promoteur du gène PDGFB.
PCT/CN2020/099603 2019-11-28 2020-06-30 Procédé de construction de plasmide rapporteur d'activité de promoteur de pdgfb WO2021103528A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201911187386.0A CN110777146A (zh) 2019-11-28 2019-11-28 一种pdgfb启动子活性报告质粒的构建方法
CN201911187386.0 2019-11-28

Publications (2)

Publication Number Publication Date
WO2021103528A1 WO2021103528A1 (fr) 2021-06-03
WO2021103528A9 true WO2021103528A9 (fr) 2021-07-15

Family

ID=69392961

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2020/099603 WO2021103528A1 (fr) 2019-11-28 2020-06-30 Procédé de construction de plasmide rapporteur d'activité de promoteur de pdgfb

Country Status (2)

Country Link
CN (1) CN110777146A (fr)
WO (1) WO2021103528A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110777146A (zh) * 2019-11-28 2020-02-11 南通大学 一种pdgfb启动子活性报告质粒的构建方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928939A (en) * 1995-03-01 1999-07-27 Ludwig Institute For Cancer Research Vascular endothelial growth factor-b and dna coding therefor
US7341847B2 (en) * 2003-04-02 2008-03-11 Agency For Science, Technology And Research Promoter construct for gene expression in neuronal cells
CN101481706A (zh) * 2008-01-12 2009-07-15 上海中医药大学附属普陀医院 含有mdr1基因启动子和报告基因的重组质粒及其用途
CN103509811A (zh) * 2013-08-07 2014-01-15 贵州大学 牛脂肪酸转运蛋白1基因启动子荧光素酶质粒的构建方法
CN103525940A (zh) * 2013-10-29 2014-01-22 郑州大学 检验cyp3a4内含子snp具有增强子和启动子功能的报告基因方法
CN109517807A (zh) * 2018-11-20 2019-03-26 暨南大学 一种心脏血管靶向的噬菌体载体及其用途
CN109576297B (zh) * 2018-12-27 2022-06-24 上海中医药大学附属曙光医院 一种含wsb1基因启动子和报告基因的重组质粒及其构建方法和应用
CN110305901A (zh) * 2019-07-08 2019-10-08 云南大学 一种基于人tlr4基因启动子区的双荧光素酶报告基因载体及其构建方法与应用
CN110777146A (zh) * 2019-11-28 2020-02-11 南通大学 一种pdgfb启动子活性报告质粒的构建方法

Also Published As

Publication number Publication date
CN110777146A (zh) 2020-02-11
WO2021103528A1 (fr) 2021-06-03

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