Classifications

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  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
  • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
  • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
  • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
  • C07K16/283—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against Fc-receptors, e.g. CD16, CD32, CD64
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
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  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/52—Cytokines; Lymphokines; Interferons
  • C07K14/54—Interleukins [IL]
  • C07K14/5443—IL-15
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
  • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
  • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K39/00—Medicinal preparations containing antigens or antibodies
  • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
  • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
  • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
  • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
  • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
  • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
  • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
  • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
  • C07K2317/622—Single chain antibody (scFv)
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2317/00—Immunoglobulins specific features
  • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
  • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2319/00—Fusion polypeptide
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K2319/00—Fusion polypeptide
  • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
  • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Definitions

• a multispecific immunotherapeutic compound that includes an NK cell engaging domain, an NK activating domain and a targeting domain.
• the targeting domain selectively binds to HER2, HER3, or the HER2/HER3 heterodimer complex and is operably linked to the NK activating domain and the NK cell engaging domain.
• the NK cell engaging domain specifically binds to CD16.
• the CD16 can be CD16a or CD16b.
• the NK cell engaging domain includes the amino acid sequence of SEQ ID NO:2.
• the NK cell engaging domain moiety can include an antibody or a binding fragment thereof.
• the antibody or a binding fragment thereof can be human, humanized, or camelid.
• the NK activating domain includes an IL-15 component.
• the IL-15 component includes the amino acid sequence of SEQ ID NO:4 or a functional variant thereof.
• the functional variant of IL-15 includes an N72D or N72A amino acid substitution as compared to SEQ ID NO:4.
• the targeting domain includes an antibody or a binding fragment thereof.
• the antibody binding fragment can include an scFv, a F(ab)2, a Fab, or a single-domain antibody fragment.
• the targeting domain includes the amino acid sequence of SEQ ID NO:6, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, or SEQ ID NO:27.
• the immunotherapeutic compound can include a second targeting domain.
• the immunotherapeutic compound can include a second NK cell engaging domain.
• the immunotherapeutic compound can include a second NK activating domain.
• this disclosure describes a composition that includes any embodiment of the therapeutic compound summarized above and a pharmaceutically acceptable carrier.
• the composition can further include an additional therapeutic agent.
• the additional therapeutic agent can include a chemotherapeutic agent.
• the additional therapeutic agent can include a therapeutic agent that targets HER2, HER3, or the HER2/HER3 heterodimer complex.
• this disclosure describes a method that includes administering to a subject any embodiment of the compound or composition summarized above in an amount effective to induce NK-mediated killing of a cancer cell.
• this disclosure describes a method for stimulating expansion of NK cells in vivo.
• the method includes administering to a subject an amount of any embodiment of the compound or composition summarized above effective to stimulate expansion of NK cells in the subject.
• this disclosure describes a method of treating cancer in a subject.
• the method includes administering to the subject an amount of any embodiment of the compound or composition summarized above effective for treating the cancer.
• the compound or composition is administered prior to, simultaneously with, or following chemotherapy, surgical resection of a tumor, or radiation therapy.
• the chemotherapy can include altretamine, amsacrine, L- asparaginase, colaspase, bleomycin, busulfan, capecitabine, carboplatin, carmustine, chlorambucil, cisplatin, cladribine, cyclophosphamide, cytophosphane, cytarabine, dacarbazine, dactinomycin, daunorubicin, docetaxel, doxorubicin, epirubicin, etoposide, fluorouracil, fludarabine, fotemustine, ganciclovir, gemcitabine, hydroxyurea, idarubicin, ifosfamaide, irinotecan, lomustine, melphalan, mercaptopurine, methotrexate, mitoxantrone, mitomycin C, nimustine, oxaliplatin, paclitaxel, pemetrexed
• FIG.2 Schematic illustration of an exemplary expression vector with arrangement of coding regions that encode components of cam1615HER2 (left to right): camelid anti-CD16 VHH, human IL-15, anti-HER2 scFV.
• B Gene map of the exemplary expression vector encoding cam1615HER2, including restriction sites and target gene positions on the pET28c vector.
• FIG.2. Production and purification of cam1615HER2.
• A SDS-PAGE gel stained with Coomassie Blue dye, indicating the purity and size of the final product after the two orthogonal column steps. MWS: molecular weight standards; NR: nonreduced; R: Reduced. Densitometry was performed to derive the final purity.
• FIG.3 Chromatography trace resulting from the first-step purification of cam1615HER2 on an ion exchange (FFQ) column. The collection peak is indicated by the double-sided arrow.
• C Chromatography trace of resulting from the second-step purification of cam1615HER2 on size exclusion column. The collection peak is indicated by the double-sided arrow.
• cam1615HER2 treatment shows percentage of highly proliferating NK cells were significantly different when compared to the controls.
• cam1615HER2 treatment shows percentage of total NK cells were significantly different when compared to the controls.
• cam1615HER2 treatment shows numbers of raw NK counts were significantly different when compared to the NT control.
• cam1615HER2 treatment did not increase percentage of highly proliferating CD3 + CD56- T cells.
• cam1615HER2 treatment did not increase percentage of total CD3 + CD56- T cells.
• cam1615HER2 treatment did not increase numbers of raw CD3 + CD56- T cell.
• BAC3 is a CD3 binding molecule and serves as a negative control for binding.
• FIG.6 Testing of the ability of TriKE to augment the killing of drug (tamoxifen) resistant MCF-7L-TamR breast carcinoma cells.
• A Background cytotoxic activity of CD56 + CD3- NK cells without cancer cells was tested using CD107a flow cytometry.
• F Intracellular IFN- y activity using PBMCs incubated with the parental MCF-7L cell line.
• FIG.7 Intracellular IFN- y activity using PBMCs incubated with SKBR-3 breast cancer cells. Intracellular IFN- y activity correlated with CD107a activity.
• FIG.7 INCUCYTE (Essen Bioscience, Inc., Ann Arbor, MI) data measuring killing in real time of SKOV3 ovarian cancer cells in the presence of PBMCs, confirming CD107a cytotoxicity data.
• A Spheroid size;
• B spheroid intensity.
• Cam1615HER2 causes a precipitous decline in target cells measured over a 120-hour period compared to lesser activity in no treatment, anti-cam16 alone (CAM16), and IL-15 alone (IL15) controls.
• N 7 donors/group.
• A CD107a background activity when cells from patient ascites fluid were incubated without MA-148 ovarian cancer cells.
• B CD107a activity when ascites cells were incubated with MA- 148 cells ovarian cancer cells.
• C CD107a background activity when cells from normal donors were incubated without MA-148 ovarian cancer cells.
• E IFN- y background activity when cells from patient ascites fluid were incubated without MA-148 ovarian cancer cells.
• F IFN- y activity when ascites cells were incubated with MA-148 cells ovarian cancer cells.
• G IFN- y background activity when cells from normal donors were incubated without MA-148 ovarian cancer cells.
• A CD107a activity when PBMC NK cells were incubated with OVCAR3 ovarian cancer cells.
• B CD107a activity when PBMC NK cells were incubated with OVCAR5 ovarian cancer cells.
• C CD107a activity when PBMC NK cells were incubated with SKOV3 ovarian cancer cells.
• D IFN- y activity when PBMC NK cells were incubated with OVCAR3 ovarian cancer cells.
• E IFN- y activity when PBMC NK cells were incubated with OVCAR5 ovarian cancer cells.
• F IFN- y activity when PBMC NK cells were incubated with SKOV3 ovarian cancer cells.
• SKOV3 data were performed with the following negative controls: no treatment (NT), anti-cam16 alone (CAM16), IL-15 (IL15), and anti-HER2 antibody alone (e23).
• NT no treatment
• CAM16 anti-cam16 alone
• IL15 IL-15
• e23 anti-HER2 antibody alone
• HER2 has a direct association with cancer since its overexpression is associated with poor prognosis in breast cancer and it triggers intracellular signaling pathways related to cell proliferation, differentiation, and survival.
• HER2 has a direct association with cancer since its overexpression is associated with poor prognosis in breast cancer and it triggers intracellular signaling pathways related to cell proliferation, differentiation, and survival.
• the immunotherapeutic compound can be a Trispecific Killer Engager compound (TriKEs).
• TriKEs have three separate binding regions: an NK cell engaging domain that binds to an NK cell (e.g., CD16), an NK activating domain that includes a cytokine or a functional fragment thereof that binds to a receptor for that cytokine, and a targeting domain that binds a marker present on a target cell (e.g., a cancer cell).
• a target cell e.g., a cancer cell.
• TriKEs offer the advantage of combining an antibody-dependent cellular cytotoxicity (ADCC)-facilitating moiety and an expansion-related moiety (IL-15) on the same molecule.
• ADCC antibody-dependent cellular cytotoxicity
• IL-15 expansion-related moiety
• One or more of the binding regions or domains in the immunotherapeutic compound can include an antibody.
• antibody refers generally an immunoglobulin or a fragment thereof and thus encompasses a monoclonal antibody, a fragment thereof.
• Exemplary antibody fragments include, but are not limited to, an scFv, a Fab, a F(ab’)2, an Fv, a single- domain Ab (sdAb), or other modified forms (e.g., humanized), and/or a combination of monoclonal antibodies and/or fragments thereof.
• the NK cell engaging domain is a camelid single-domain antibody fragment.
• the immunotherapeutic compound has a targeting domain that includes a HER2-targeting scFv having the amino acid sequence of SEQ ID NO:6, the immunotherapeutic compounds described herein can include any other suitable HER2-targeting and or HER3-targeting moiety.
• the targeting domain can recognize HER2, HER3, and/or the HER2/HER3 heterodimer.
• HER2/HER3 heterodimers are detected in many breast cancers and many HER2 + tumors.
• HER2/HER3 dimers are associated with proliferation, distant metastasis, and/or poor patient outcome.
• Exemplary alternative targeting moieties include, antibodies and antibody fragments that specifically bind to HER2, HER3, and/or the HER2/HER3 heterodimer.
• Exemplary antibody fragments include but are not limited to, e23 or a functional fragment thereof (e.g., SEQ ID NO:15), trastuzumab or a functional fragment thereof (e.g., SEQ ID NO:16 and European Patent No.
• EP 3457139 A1 SEQ ID NO:17, SEQ ID NO:18, lumretuzumab or a functional fragment thereof (RG7116; Liu et al., 2019, Biol Proced Online 21:5; e.g., SEQ ID NO:19, SEQ ID NO:20), seribantumab or a functional fragment thereof (MM-121; Liu et al., 2019, Biol Proced Online 21:5; e.g., SEQ ID NO:21 or SEQ ID NO:22), KTN3379/CDX-3379 or a functional fragment thereof (Liu et al., 2019, Biol Proced Online 21:5; e.g., SEQ ID NO:23), patritumab or a functional fragment thereof (U3-1287; Liu et al., 2019, Biol Proced Online 21:5; e.g., SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, or SEQ ID NO:27
• an immunotherapeutic compound can include any other suitable NK engaging moiety.
• exemplary alternative NK engaging moieties include, but are not limited to, any amino acid sequence that can selectively bind to a receptor at least partially located at the surface of an NK cell.
• the NK cell engaging domain can serve a function of binding an NK cell and thereby bring the NK into spatial proximity with a target to which the targeting domain selectively binds.
• the NK cell engaging domain can selectively bind to a receptor that activates the NK cell and, therefore, also possess an activating function.
• the NK cell engaging domain of the exemplary cam1615HER2 compound possesses NK activating activity.
• the NK cell engaging domain may interrupt mechanisms that inhibit NK cells.
• the NK cell engaging domain can include, for example, anti-PD-1/PD-L1, anti-NKG2A, anti-TIGIT, anti-killer-immunoglobulin receptor (KIR), and/or any other inhibition blocking domain.
• the NK cell engaging domain can include an antibody or ligand that selectively binds to any NK cell receptor such as, for example, the cell cytotoxicity receptor 2B4, low affinity Fc receptor CD16, killer immunoglobulin like receptors (KIR), CD2, NKG2A, TIGIT, NKG2C, LIR-1, and/or DNAM-1.
• NK cell receptor such as, for example, the cell cytotoxicity receptor 2B4, low affinity Fc receptor CD16, killer immunoglobulin like receptors (KIR), CD2, NKG2A, TIGIT, NKG2C, LIR-1, and/or DNAM-1.
• KIR killer immunoglobulin like receptors
• CD2 killer immunoglobulin like receptors
• NKG2A killer immunoglobulin like receptors
• TIGIT TIGIT
• NKG2C TIGIT
• LIR-1 LIR-1
• DNAM-1 DNAM-1.
• CD16 has been identified as Fc receptors FcgRIIIa (CD16a) and Fc
• Anti-CD16 antibodies selectively bind to NK cells, but also can bind to neutrophils.
• Anti-CD16a antibodies selectively bind to NK cells, but do not bind to neutrophils.
• An immunotherapeutic compound that includes an NK cell engaging domain having an anti-CD16a antibody can bind to NK cells but not bind to neutrophils. Thus, in circumstances where one may want to engage NK cells but not engage neutrophils, one can design the NK cell engaging domain of the immunotherapeutic compound to include an anti-CD16a antibody.
• the NK activating domain can include any amino acid sequence that activates NK cells, promotes sustaining NK cells, or otherwise promotes NK cell activity.
• the NK activating domain can be, or can be derived from, one or more cytokines that can activate and/or sustain NK cells.
• the term “derived from” refers to an amino acid fragment of a cytokine (e.g., IL-15) that is a functional variant of the referenced cytokine—i.e., has sufficient sequence similarity or sequence identity to the referenced cytokine to provide NK cell activating and/or sustaining activity.
• cytokines on which an NK activating domain may be based include, for example, IL-15, IL-18, IL-12, and IL-21.
• a cam1615HER2 compound includes an NK activating domain derived from IL-15
• a HER2-targeting compound may be designed to have an NK activating domain that is, or is derived from, any suitable cytokine.
• reference to an NK activating domain by identifying the cytokine on which it is based can refer to either the full amino acid sequence of the cytokine or a functional variant of the cytokine.
• a functional variant of the cytokine can include any suitable amino acid fragment of the cytokine and/or a modified version of the cytokine that includes one or more amino acid deletions, additions, and/or substitutions.
• NK activating domain includes an NK activating domain that includes the full amino acid sequence of IL-15, an NK activating domain that includes a fragment of IL-15, or an NK activating domain such as, for example, IL-15N72D or IL-15N72A, that includes an amino acid substitution compared to the wild-type IL-15 amino acid sequence.
• the compositions and methods described herein can involve the use of immunotherapeutic compound modified to include additional domains.
• the immunotherapeutic compound can be designed to be a larger molecule, having more than one targeting domain, more than one NK cell engaging domain, and/or more than one NK activating domain.
• the NK activating domains may be provided in series or in any other combination.
• Any cytokine-based NK activating domain can include either the full amino acid sequence of the cytokine, may be an amino acid fragment, or may be a modified version of the cytokine, independent of the nature of other NK activating domains included in the immunotherapeutic compound.
• Exemplary additional targeting domains include, but are not limited to, any moiety that selectively binds to an intended target such as, for example, a tumor cell, a target in the cancer stroma, a target on an inhibitory cell such as myeloid derived suppressor cells that are CD33+, or a target on a virally-infected cell.
• a targeting domain can include, for example, an anti- tumor antibody such as rituximab (anti-CD20), afutuzumab (anti-CD20), pertuzumab (anti- HER2/neu), labetuzumab (anti-CEA), adecatumumab (anti-EpCAM), citatuzumab communicatingox (anti- EpCAM), edrecolomab (anti-EpCAM), arcitumomab (anti-CEA), bevacizumab (anti-VEGF-A), cetuximab (anti-EGFR), nimotuzumab (anti-EGFR), panitumumab (anti-EGFR), zalutumumab (anti-EGFR), gemtuzumab ozogamicin (anti-CD33), lintuzumab (anti-CD33), etaracizumab (anti-integrin a v b 3 ), intetumum
• An amino acid sequence is a “functional variant” of a reference amino acid sequence if the “functional variant” amino acid sequence possesses a specified amount of sequence identity or sequence specificity compared to the reference amino acid sequence.
• An amino acid sequence is a “functional fragment” of a reference amino acid sequence if the “functional fragment” amino acid sequence contains less than the full-length amino acid sequence of the reference amino acid sequence.
• a “functional fragment” may further possess a specified amount of sequence identity or sequence specificity compared to the reference amino acid sequence.
• Sequence similarity and/or sequence identity of two amino acid sequences can be determined by aligning the residues of the amino acid sequences to optimize the number of identical amino acids along the lengths of their sequences; gaps in either or both sequences are permitted in making the alignment in order to optimize the number of identical amino acids, although the amino acids in each sequence must nonetheless remain in their proper order.
• a pair-wise comparison analysis of amino acid sequences can be carried out using the BESTFIT algorithm in the GCG package (version 10.2, Madison WI).
• polypeptides may be compared using the Blastp program of the BLAST 2 search algorithm, as described by Tatiana et al., (FEMS Microbiol Lett, 174, 247-250 (1999)), and available on the National Center for Biotechnology Information (NCBI) website.
• structural similarity may be referred to by percent “identity” or may be referred to by percent “similarity.” “Identity” refers to the presence of identical amino acids. “Similarity” refers to the presence of not only identical amino acids but allows for the presence of conservative substitutions. A conservative substitution for an amino acid residue within an amino acid sequence may be selected from other members of the class to which the amino acid residue belongs.
• nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and tyrosine.
• Polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine and glutamine.
• the positively charged (basic) amino acids include arginine, lysine and histidine.
• the negatively charged (acidic) amino acids include aspartic acid and glutamic acid.
• conservative substitutions include, for example, Lys for Arg and vice versa to maintain a positive charge; Glu for Asp and vice versa to maintain a negative charge; Ser for Thr so that a free -OH is maintained; and Gln for Asn to maintain a free -NH2.
• an amino acid belonging to a grouping of amino acids having a particular size or characteristic e.g., charge, hydrophobicity, or hydrophilicity
• Regions within an amino acid sequence that are not directly associated with biological activity may be deduced from alignment analyses, identifying regions where variability (e.g., additions, deletions, or non-conservative substitutions) are present when comparing related amino acid sequences.
• An NK engaging domain, an NK activating domain, or a targeting domain can include an amino acid sequence with at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence similarity to a reference amino acid sequence (e.g., a reference antibody fragment, cytokine, or cytokine fragment).
• a reference amino acid sequence e.g., a reference antibody fragment, cytokine, or cytokine fragment
• An NK engaging domain, an NK activating domain, or a targeting domain can include an amino acid sequence with at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the reference amino acid sequence.
• An immunotherapeutic compound as described herein also can be designed to provide additional sequences, such as, for example, the addition of added C-terminal or N-terminal amino acids that can, for example, facilitate purification by trapping on columns or use of antibodies.
• Such tags include, for example, histidine-rich tags that allow purification of polypeptides on nickel columns.
• Histidine-rich tags that allow purification of polypeptides on nickel columns.
• Such gene modification techniques and suitable additional sequences are well known in the molecular biology arts.
• This disclosure also provides polynucleotides that encode any of the immunotherapeutic compounds described herein, and the complements of such polynucleotide sequences. Given the amino acid sequence of any one of the immunotherapeutic compounds polypeptides described herein (or one or more component fragments of the immunotherapeutic compound), a person of ordinary skill in the art can determine the full scope of polynucleotides that encode that amino acid sequence using conventional, routine methods.
• FIG.1A shows an exemplary construction of an exemplary embodiment of second generation TriKE, referred to herein as cam1615HER2 (SEQ ID NO:1), that is capable of both antibody-dependent cellular cytotoxicity (ADCC) and NK cell expansion.
• the cam1615HER2 TriKE includes an anti-CD16 VHH as the NK cell engaging domain.
• the anti-CD16 VHH is the variable region of the heavy chain of a camelid antibody.
• FIG.1B is a plasmid map showing placement of the TriKE coding sequences in a pET expression vector.
• FIG.2B shows the absorbance tracing of the fractions of bacterial and target protein as it passes over FFQ ion exchange column as the first phase of purification from inclusion bodies.
• FIG.2C shows the absorbance tracing from the second purification phase, size exclusion chromatography (SEC). The double-sided arrow shows the target peak collected as cam1615HER2 exited the column.
• FIG.2A shows the final product (fractions C2-D4) as mostly a single band when analyzed using SDS-PAGE with Coomasie Blue staining providing evidence of a uniform product. The final product was greater than 90% pure with a molecular weight of about 55 kDa.
• the cam1615HER2 TriKE can be constructed in other ways, however. It is possible to design multiple constructs, each of which encodes and directs synthesis of a portion of the complete immunotherapeutic compound.
• an anti-HER2 light chain (e.g., SEQ ID NO:17) may be encoded on one plasmid and an anti-HER2 heavy chain (e.g., SEQ ID NO:18) may encoded on a second plasmid.
• an anti-HER2 light chain and the anti-HER2 heavy chain can dimerize to form an anti-HER2 Fab as the targeting moiety of the immunotherapeutic compound.
• a cam1615HER2 TriKE can be constructed in this manner.
• SEQ ID NO:31 provides the amino acid sequence expressed from an exemplary first plasmid, the amino acid sequence containing a signal peptide, the anti-HER2 light chain, a linker, an IL-15 amino acid sequence, a second linker, and a camelid anti-CD16 single domain antibody fragment.
• SEQ ID NO:32 provides the amino acid sequence expressed from an exemplary second plasmid, the amino acid sequence containing a signal peptide and the anti-HER2 heavy chain.
• a single plasmid construct can include all of the components of the complete immunotherapeutic compound.
• SEQ ID NO:33 provides the amino acid sequence expressed from an exemplary single-plasmid construct, in which the amino acid sequence includes a signal sequence, the anti-HER2 heavy chain fragment, a T2A self-cleaving peptide, a second signal sequence, the anti-HER2 light chain fragment, a linker, an IL-15 amino acid sequence, a second linker, and a camelid anti-CD16 single domain antibody fragment.
• the T2A peptide self-cleaves, separating the anti-HER2 heavy chain fragment from the remainder of the immunotherapeutic compound so that it is able to dimerize with the anti-HER2 light chain fragment.
• FIG.3A and FIG.3B show the percentage of highly proliferated CD56 + CD3- NK cells and the total percentage of NK cells after seven days of incubation with 50 nM cam1615HER2 TriKE compared to untreated control (NT) and the IL-15 control. Both were significantly elevated after incubation with cam1615HER2. Likewise, exposure to cam1615HER2 also significantly enhanced total NK count compared to the NT control (FIG.3C).
• FIG.3D-F show that CD3 + CD56- T cell percentages and raw numbers were not elevated compared to the untreated controls. Together, these studies indicate that the HER2 TriKE stimulates expansion of NK cells but not T cells. The data also show that the IL-15 moiety within the TriKE is functional and in a viable conformational alignment. Cytotoxicity is a hallmark of NK immunotherapy. To establish the antibody-dependent cellular cytotoxicity (ADCC) potency, various cell lines were analyzed for CD107a expression, an accepted measure of NK cell cytotoxicity. The cam1615HER2 TriKE was tested against SKOV3 and SK-BR-3 since breast cancer and some ovarian cancer cases are known to overexpress ERBB2, rendering it a desirable target for antibody-directed targeting.
• ADCC antibody-dependent cellular cytotoxicity
• UMSCC-11B cell line was tested as a negative control since it has minimal expression of HER2.
• binding was measured by FITC labeling the various agents and then testing their direct binding to the targets by flow cytometry.
• SKOV3 (FIG.4A) and SK-BR-3 (FIG.4B) showed the highest level of HER2 TriKE binding.
• UMSCC-11B showed a lower level of binding (FIG.4C).
• ADCC activity was measured, the cam1615HER2 TriKE showed highly elevated CD107a activity against SKOV3 and SK-BR-3 cell lines compared to IL-15 and untreated controls (FIG.5A, 5C).
• FIG.5E shows CD107a effector cell background without added target cells.
• FIG.6B shows CD107a activity when targets were added. The highest level of killing was observed with the cam1615HER2 TriKE compared to controls. Cam16 itself did have activity, but not as high.
• MCF-7L-TamR A subline of MCF-7L (MCF-7L-TamR) is tamoxifen resistant and showed a similar pattern of CD107a activity when treated with the cam1615HER2 TriKE (FIG.6C).
• NK cells when activated to kill, also are known for secretion of anti-cancer cytokines such as IFN-g.
• FIG.6E shows IFN-g levels, quantitated in the same samples by intracellular staining, were elevated with the cam1615HER2 TriKE and minimally affected in the controls, indicating NK cell activation. The same was true for MCF-7L-TamR (FIG.6F).
• FIG.6D confirms the cam1615HER2 TriKE killing of SK-BR-3 cells and indicates that it kills as well as trastuzumab.
• FIG.6H shows that the cam1615HER2 TriKE has even greater IFN- y ⁇ enhancing activity than trastuzumab. Together, these data show that HER2 is a valid target for immune engagers on human breast cancer cells and that innate immunotherapy is highly effective in vitro against drug resistant breast cancer cell lines.
• FIG.12 provides data showing activity of cam1615HER2 TriKE against OVCAR-3 and OVCAR-5, two additional cell lines that express HER2.
• cam1615HER2 TriKE shows elevated CD107a activity (FIG.12A, 12B) and IFN-g activity (FIG.12D, 12E) compared to controls.
• a repeat experiment evaluating SKOV3 is included with a more extensive number of negative controls including anti-HER2 scFv alone (e23), cam16 VHH alone, IL-15 alone, and no treatment control.
• drug showed elevated CD107a activity (FIG.12C) and elevated IFN-g activity (FIG.12F) compared to controls. Killing was further evaluated in real time using the INCUCYTE ZOOM platform (Essen Bioscience, Inc., Ann Arbor, MI) over two days.
• SKOV3, grown in culture as spheroids were studied.
• SKOV3, stably transduced with NUCLIGHT RED (Essen Bioscience, Inc., Ann Arbor, MI) was incubated with enriched NK cells and cam1615HER2 TriKE, free IL-15, cam16 VHH alone, or no treatment. Caspase 3/7 green reagent was added to detect cell death. Dying cells become green and dying NUCLIGHT RED Raji cells become yellow, allowing for tracking of remaining living cells.
• cam1615HER2 TriKE induced an impressive drop in SKOV3 spheroid size (FIG.7A) and spheroid intensity (FIG.7B) compared to the IL-15, cam16 VHH alone, and untreated controls over the 72 hours of continuous measurement.
• FIG.8 provides images showing that cam1615HER2 causes a precipitous time- dependent decline in target cells measured over a 72-hour period. Findings in this direct-kill assay correlated with findings in the CD107a assay.
• NK cells were obtained from six consented ovarian cancer patients instead of normal volunteers and tested against MA-148 ovarian cancer cells.
• FIG.9A shows lower CD107a background upon treatment of effectors without cancer cells than with cam1615HER2 TriKE or controls.
• FIG.9B shows significant elevation in CD107a expression in effectors plus tumor targets upon treatment with cam1615HER2 TriKE compared to untreated controls.
• FIG.9E shows low activity in effectors without targets when treated with the cam1615HER2 TriKE.
• the cam1615HER2 TriKE shows enhanced IFN- y activity (FIG. 9F).
• FIG.9C shows CD107a background of normal donor effector cells
• FIG.9D shows CD107a activity of normal donor cells with MA148 cancer cells.
• FIG.9G shows IFN- y ⁇ background of normal donor effector cells
• FIG.9H shows IFN- y activity of normal donor cells with MA148 cancer cells.
• FIG.10 shows data demonstrating in vivo efficacy of the cam1615HER2 TriKE using a SCID/hu/NK xenograft model.
• FIG.10A and 10B show visual imaging data of the untreated (FIG.10A) and treated (FIG.10B) groups of mice about six weeks after intraperitoneal inoculation with SKOV3 tumor cells. Advanced tumor progression was obvious in the untreated group of mice (FIG.10A) and clearly reduced in the group of treated mice (FIG.10B).
• FIG.11A shows minimal change in animal weight over a 46-day period, indicating that the cam1615HER2 TriKE is not toxic despite daily administration.
• FIG.11B is a comparative snapshot of total tumor bioluminescence (total flux) from two independent experiments and shows that even on day 46 after tumor administration, tumor growth is significantly inhibited in the treated versus the untreated group.
• FIG.11C shows the overall progress of tumor growth (bioluminescence) in time. Over the 46-day period, tumor growth is significantly inhibited in the treated group, but tumor growth does begin to reoccur at day 39.
• FIG.11D shows longer term results in survival plot. Five of six animals in the untreated group died by day 52 and all untreated animals dies by day 60. In contrast, here is still a 50% survivorship by day 72 in the cam1615HER2-treated group.
• cam1615HER2-TriKE-treated group still showed tumor load, so treatment was inhibitory, not curative.
• an immunotherapeutic compound such as a trispecific killer engager (TriKE) compound.
• An exemplary immunotherapeutic compound is effective against tamoxifen refractory cells and thus may readily kill cancer cells resistant to tamoxifen or other chemotherapeutic agents.
• an exemplary immunotherapeutic compound a HER2-targeting NK engaging TriKE
• the data presented herein further provide a basis for immunotherapeutic compounds that target HER3 and/or the HER2/HER3 heterodimer.
• An immunotherapeutic compound described herein may be formulated with a pharmaceutically acceptable carrier.
• carrier includes any solvent, dispersion medium, vehicle, coating, diluent, antibacterial, and/or antifungal agent, isotonic agent, absorption delaying agent, buffer, carrier solution, suspension, colloid, and the like.
• carrier includes any solvent, dispersion medium, vehicle, coating, diluent, antibacterial, and/or antifungal agent, isotonic agent, absorption delaying agent, buffer, carrier solution, suspension, colloid, and the like.
• the use of such media and/or agents for pharmaceutical active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients also can be incorporated into the compositions.
• pharmaceutically acceptable refers to a material that is not biologically or otherwise undesirable, i.e., the material may be administered to an individual along with an immunotherapeutic compound without causing any undesirable biological effects or interacting in a deleterious manner with any of the other components of the pharmaceutical composition in which it is contained.
• An immunotherapeutic compound may therefore be formulated into a pharmaceutical composition.
• the pharmaceutical composition may be formulated in a variety of forms adapted to a preferred route of administration.
• a composition can be administered via known routes including, for example, oral, parenteral (e.g., intradermal, transcutaneous, subcutaneous, intramuscular, intravenous, intraperitoneal, etc.), or topical (e.g., intranasal, intrapulmonary, intramammary, intravaginal, intrauterine, intradermal, transcutaneous, rectally, etc.).
• a pharmaceutical composition can be administered to a mucosal surface, such as by administration to, for example, the nasal or respiratory mucosa (e.g., by spray or aerosol).
• a composition also can be administered via a sustained or delayed release.
• the composition is administered intraperitoneally, intravenously, or subcutaneously.
• an immunotherapeutic compound may be provided in any suitable form including but not limited to a solution, a suspension, an emulsion, a spray, an aerosol, or any form of mixture.
• the composition may be delivered in formulation with any pharmaceutically acceptable excipient, carrier, or vehicle.
• the formulation may be delivered in a conventional topical dosage form such as, for example, a cream, an ointment, an aerosol formulation, a non- aerosol spray, a gel, a lotion, and the like.
• the formulation may further include one or more additives including such as, for example, an adjuvant, a skin penetration enhancer, a colorant, a fragrance, a flavoring, a moisturizer, a thickener, and the like.
• the composition may is formulated into a solution or a suspension.
• a formulation may be conveniently presented in unit dosage form and may be prepared by methods well known in the art of pharmacy. Methods of preparing a composition with a pharmaceutically acceptable carrier include the step of bringing an immunotherapeutic compound into association with a carrier that constitutes one or more accessory ingredients.
• a formulation may be prepared by uniformly and/or intimately bringing the active molecule into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product into the desired formulations.
• this disclosure describes a method of treating cancer in a subject. Generally, the method includes administering to the subject an amount of the immunotherapeutic compound effective for treating the cancer.
• Treatment refers to reducing, limiting progression, ameliorating, or resolving, to any extent, the symptoms or signs related to a condition.
• “ameliorate” refers to any reduction in the extent, severity, frequency, and/or likelihood of a symptom or clinical sign characteristic of a particular condition
• symptom refers to any subjective evidence of disease or of a patient’s condition
• signal or “clinical sign” refers to an objective physical finding relating to a particular condition capable of being found by one other than the patient.
• a “treatment” may be therapeutic or prophylactic.
• “Therapeutic” and variations thereof refer to a treatment that ameliorates one or more existing symptoms or clinical signs associated with a condition.
• “Prophylactic” and variations thereof refer to a treatment that limits, to any extent, the development and/or appearance of a symptom or clinical sign of a condition.
• a “therapeutic” treatment is initiated after the condition manifests in a subject, while “prophylactic” treatment is initiated before a condition manifests in a subject.
• the method can involve prophylactic treatment of a subject at risk of developing a condition.
• “At risk” refers to a subject that may or may not actually possess the described risk.
• a subject “at risk” for developing a specified condition is a subject that possesses one or more indicia of increased risk of having, or developing, the specified condition compared to individuals who lack the one or more indicia, regardless of the whether the subject manifests any symptom or clinical sign of having or developing the condition.
• Exemplary indicia of a condition can include, for example, genetic predisposition, ancestry, age, sex, geographical location, lifestyle, or medical history. Treatment may also be continued after symptoms have resolved, for example to prevent or delay their recurrence. Accordingly, an immunotherapeutic compound may be administered to the subject before, during, or after the subject first exhibits a symptom or clinical sign of the condition.
• Treatment initiated before the subject first exhibits a symptom or clinical sign associated with the condition may result in decreasing the likelihood that the subject experiences clinical evidence of the condition compared to a subject to which the immunotherapeutic compound is not administered, decreasing the severity of symptoms and/or clinical signs of the condition, and/or completely resolving the condition.
• Treatment initiated after the subject first exhibits a symptom or clinical sign associated with the condition may result in decreasing the severity of symptoms and/or clinical signs of the condition compared to a subject to which the immunotherapeutic compound is not administered, and/or completely resolving the condition.
• the amount of the immunotherapeutic compound administered can vary depending on various factors including, but not limited to, the specific immunotherapeutic compound being administered, the weight, physical condition, and/or age of the subject, and/or the route of administration.
• the absolute weight of immunotherapeutic compound included in a given unit dosage form can vary widely, and depends upon factors such as the species, age, weight and physical condition of the subject, and/or the method of administration. Accordingly, it is not practical to set forth generally the amount that constitutes an amount of immunotherapeutic compound effective for all possible applications. Those of ordinary skill in the art, however, can readily determine the appropriate amount with due consideration of such factors.
• the method can include administering sufficient immunotherapeutic compound to provide a dose of, for example, from about 100 ng/kg/day to about 10 mg/kg/day to the subject, although in some embodiments the methods may be performed by administering an immunotherapeutic compound in a dose outside this range.
• the method can includes administering sufficient immunotherapeutic compound to provide a minimum dose of at least 100 ng/kg/day such as, for example, at least 1 mg/kg/day, at least 5 mg/kg/day, at least 10 mg/kg/day, at least 25 mg/kg/day, at least 50 mg/kg/day, at least 100 mg/kg/day, at least 200 mg/kg/day, at least 300 mg/kg/day, at least 400 mg/kg/day, at least 500 mg/kg/day, at least 600 mg/kg/day, at least 700 mg/kg/day, at least 800 mg/kg/day, at least 900 mg/kg/day, or at least 1 mg/kg/day.
• sufficient immunotherapeutic compound to provide a minimum dose of at least 100 ng/kg/day such as, for example, at least 1 mg/kg/day, at least 5 mg/kg/day, at least 10 mg/kg/day, at least 25 mg/kg/day, at least 50 mg/kg/day, at least 100 mg/kg/day, at
• the method includes administering sufficient immunotherapeutic compound to provide a maximum dose of no more than 10 mg/kg/day such as, for example, no more than 5 mg/kg/day, no more than 4 mg/kg/day, no more than 3 mg/kg/day, no more than 2 mg/kg/day, no more than 1 mg/kg/day, no more than 900 mg/kg/day, no more than 800 mg/kg/day, no more than 700 mg/kg/day, no more than 600 mg/kg/day, no more than 500 mg/kg/day, no more than 400 mg/kg/day, no more than 300 mg/kg/day, no more than 200 mg/kg/day, no more than 100 mg/kg/day, no more than 90 mg/kg/day, no more than 80 mg/kg/day, no more than 70 mg/kg/day, no more than 60 mg/kg/day, no more than 50 mg/kg/day, no more than 40 mg/kg/day, no more than 30 mg/kg/day, no more than 20 mg/kg/day such as
• the immunotherapeutic compound provides a dose of “no greater than” a specified amount when the immunotherapeutic compound is not absent but is present in an amount up to and including the specified amount.
• the method includes administering sufficient immunotherapeutic compound to provide a dose characterized by a range having endpoints defined by any a minimum dose identified above and any maximum dose that is greater than the selected minimum dose.
• the method can include administering sufficient immunotherapeutic compound to provide a dose of from about 10 ⁇ g/kg/day to about 10 mg/kg/day to the subject, a dose of from about 100 mg/kg/day to about 1 mg/kg/day, a dose of from 5 mg/kg/day to 100 mg/kg/day, etc.
• the method includes administering sufficient immunotherapeutic compound to provide a dose that is equal to any minimum dose or any maximum dose listed above.
• the method can include administering sufficient immunotherapeutic compound to provide a dose of 1 mg/kg/day, 5 ⁇ g/kg/day, 10 ⁇ g/kg/day, 25 ⁇ g/kg/day, 50 ⁇ g/kg/day, 100 ⁇ g/kg/day, 200 ⁇ g/kg/day, 500 ⁇ g/kg/day, 1 mg/kg/day, 5 mg/kg/day, etc.
• an immunotherapeutic compound may be administered, for example, from a single dose to multiple doses per week, although in some embodiments the method can be performed by administering an immunotherapeutic compound at a frequency outside this range. In certain embodiments, an immunotherapeutic compound may be administered from about once per month to about five times per week. In some embodiments, the doses indicated above, which are described in terms of the amount of immunotherapeutic compound administered over a 24-hour period, are administered in a seven-day cycle of four days of treatment and three days of rest. In some embodiments, an immunotherapeutic compound may be administered, for example, from a single dose to multiple cycles of treatment, although in some embodiments the method can be performed by administering an immunotherapeutic compound for a duration outside this range.
• the immunotherapeutic compound may be administered for three weeks. In such embodiments, each week may be a treatment cycle such as the exemplary treatment cycle described in the preceding paragraph. In other embodiments, the immunotherapeutic compound may be administered for a greater number of treatment cycles, without a gap between one set of treatment cycles and a subsequent set of treatment cycles. The gap between one set of treatment cycles and a subsequent set of treatment cycles may be a gap of one or more weeks, one or more months, or one or more years. In some embodiments, the method further includes administering one or more additional therapeutic agents. The one or more additional therapeutic agents (e.g., chemotherapeutic agents) may be administered before, after, and/or coincident to the administration of an immunotherapeutic compound.
• the one or more additional therapeutic agents e.g., chemotherapeutic agents
• an immunotherapeutic compound and the additional therapeutic agents may be co-administered.
• co-administered refers to two or more components of a combination administered so that the therapeutic or prophylactic effects of the combination can be greater than the therapeutic or prophylactic effects of either component administered alone.
• Two components may be co-administered simultaneously or sequentially.
• Simultaneously co-administered components may be provided in one or more pharmaceutical compositions. Sequential co-administration of two or more components includes cases in which the components are administered so that each component can be present at the treatment site at the same time.
• sequential co-administration of two components can include cases in which at least one component has been cleared from a treatment site, but at least one cellular effect of administering the component (e.g., cytokine production, activation of a certain cell population, etc.) persists at the treatment site until one or more additional components are administered to the treatment site.
• a co-administered combination can, in certain circumstances, include components that never exist in a chemical mixture with one another.
• the immunotherapeutic compound and the additional therapeutic agent may be administered as part of a mixture or cocktail.
• the administration of the immunotherapeutic compound may allow for the effectiveness of a lower dosage of other therapeutic modalities when compared to the administration of the other therapeutic agent or agents alone, thereby decreasing the likelihood, severity, and/or extent of the toxicity observed when a higher dose of the other therapeutic agent or agents is administered.
• Exemplary additional therapeutic agents include altretamine, amsacrine, L-asparaginase, colaspase, bleomycin, busulfan, capecitabine, carboplatin, carmustine, chlorambucil, cisplatin, cladribine, cyclophosphamide, cytophosphane, cytarabine, dacarbazine, dactinomycin, daunorubicin, docetaxel, doxorubicin, epirubicin, etoposide, fluorouracil, fludarabine, fotemustine, ganciclovir, gemcitabine, hydroxyurea, idarubicin, ifosfamaide, irinotecan, lomustine, melphalan, mercaptopurine, methotrexate, mitoxantrone, mitomycin C, nimustine, oxaliplatin, paclitaxel, pemetrexed, proc
• an additional therapeutic agent can include an additional agent that targets EpCAM including, for example, an EpCAM specific monoclonal antibody, such as, for example, catumaxomab, a monoclonal hybrid antibody targeting EpCAM and CD3.
• administering the immunotherapeutic compound to a subject can stimulate endogenous NK cells in vivo.
• an immunotherapeutic compound as a part of an in vivo method can make NK cells antigen specific with simultaneous co-stimulation, enhancement of survival, and expansion.
• the immunotherapeutic compound can be used in vitro as an adjuvant to NK cell adoptive transfer therapy.
• the term “and/or” means one or all of the listed elements or a combination of any two or more of the listed elements; the terms “comprises,” “comprising,” and variations thereof are to be construed as open ended—i.e., additional elements or steps are optional and may or may not be present; unless otherwise specified, “a,” “an,” “the,” and “at least one” are used interchangeably and mean one or more than one; and the recitations of numerical ranges by endpoints include all numbers subsumed within that range (e.g., 1 to 5 includes 1, 1.5, 2, 2.75, 3, 3.80, 4, 5, etc.). In the preceding description, particular embodiments may be described in isolation for clarity.
• Chem.284(5):3273-3284) were spliced into a universal, humanized nanobody scaffold previously shown to allow grafts of antigen-binding loops with transfer of the antigen specificity and affinity (Behar et al., 2008, Protein Engineering, Design & Selection 21(1):1-10). This new sequence was used to manufacture cam1615HER2 (SEQ ID NO:1).
• cam1615HER2 which encodes the cam1615HER2 TriKE, encodes (from 5’ end to 3’ end) a NcoI restriction site; an ATG start codon; anti- human CD16 VHH; a 20-amino-acid (aa) segment, PSGQAGAAASESLFVSNHAY (SEQ ID NO:3); human IL-15; the seven amino acid linker, EASGGPE (SEQ ID NO:5); anti- HER2 scFv (Batra et al., 1992, Proc Natl Acad Sci USA 89(13):5867-5871), and a XhoI restriction site.
• the resulting hybrid gene (SEQ ID NO:7) was spliced into the pET28c expression vector under the control of an isopropyl-D-thiogalactopyranoside (IPTG) inducible T7 promoter.
• IPTG isopropyl-D-thiogalactopyranoside
• cam1615HER2 was 1517 base pairs.
• Wild-type human IL-15 was used and not a mutated form of the cytokine.
• the Biomedical Genomics Center, University of Minnesota, St. Paul, MN verified the gene sequence and in- frame accuracy of the target gene. Purification of protein from inclusion bodies Escherichia coli strain.BL21 (DE3) (Novagen, Madison, WI) was used for protein expression after plasmid transfection.
• the bacteria were cultured overnight in 800-ml Luria broth containing 50 mg/ml kanamycin. Expression was induced by adding IPTG (Thermo Fisher Scientific, Inc., Fair Lawn, NJ) when the media reached an absorbance of 0.65 at 600 nm. Bacterial expression resulted in packaging of target protein into inclusion bodies. After expression, bacteria were harvested and then homogenized in buffer (50 mM Tris, 50 mM NaCl, and 5 mM EDTA pH 8.0) and the pellet sonicated and centrifuged.
• buffer 50 mM Tris, 50 mM NaCl, and 5 mM EDTA pH 8.0
• Pellets were removed by centrifugation. 50 mM of CuSO4 was added to the solution and then incubated at room temperature with rapid stirring for 20 hours for air-oxidization of –SH groups. Removal of SLS was performed by adding 6 M urea and 10% AG 1-X8 resin (200–400 mesh, chloride form) (Bio-Rad Laboratories, Inc., Hercules, CA) to the detergent-solubilized protein solution. The next step added 13.3 M guanidine-HCl into the protein solution, followed by incubation at 37°C for two to three hours.
• AG 1-X8 resin 200–400 mesh, chloride form
• the solution was diluted 20-fold with refolding buffer (50 mM Tris, 0.5 M L- arginine, 1 M urea, 20% glycerol, 5 mM EDTA, pH 8.0).
• refolding buffer 50 mM Tris, 0.5 M L- arginine, 1 M urea, 20% glycerol, 5 mM EDTA, pH 8.0.
• the mixture was incubated at 4°C for two days.
• the sample was dialyzed against five volumes of 20 mM Tris-HCl at pH 8.0 for 48 hours at 4°C, then eight volumes for 18 additional hours.
• the product was then purified first by fast flow Q ion exchange chromatography and then by passage over a size exclusion column (SUPERDEX 200, Cytiva, Marlborough, MA).
• Sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed using Simply Blue life Stain (Invitrogen, Carlsbad, CA) to evaluate protein size and purity.
• Cancer cell lines The following cell lines were obtained from the American Type Culture Collection (Manassas, VA): MCF-7L (ductal breast carcinoma), MCF-7L-TamR (tamoxifen-resistant subline of MCF-7), SKOV3 (ovarian ascites), SK-BR-3 (breast carcinoma derived from a metastatic site), UMSCC-11B squamous cell carcinoma derived from a larynx tumor (Worsham et al., 2006, Arch Otolaryngol Head Neck Surg 132:668-677), HL-60 (acute promyelocytic leukemia), MA-148 (ovarian carcinoma), and SKOV3-luc.
• SKOV3-luc was made by transfecting SKOV3 with a luciferase reporter construct using LIPOFECTAMINE (Invitrogen, Carlsbad, CA) and selective pressure applied with 10 ⁇ g/ml of blastocidin.
• UMSCC-11B was authenticated by STR testing performed by the Fragment Analysis Facility, John Hopkins University.
• MA148 (established locally at the University of Minnesota) is a human epithelial ovarian carcinoma cell line. Lines were maintained in RPMI 1640 RPMI supplemented with 10-20% fetal bovine serum (FBS) and 2 mmol/L L-glutamine. Lines were incubated in a humidified atmosphere containing 5% CO2 at a constant 37°C.
• FBS fetal bovine serum
• ADCC Antibody-dependent cellular cytotoxicity
• the University of Minnesota Cancer Center Tissue Procurement Facility obtained high-grade serous ascites samples from patients diagnosed with ovarian cancer following approval from the Institutional Review Board. All specimens were collected from women diagnosed with advanced-stage ovarian or primary peritoneal carcinoma at time of primary debulking surgery. Cells were pelleted, lysed to remove red blood cells, cryopreserved in 10% DMSO/90% FBS and stored in liquid nitrogen. PBMCs were incubated overnight (37°C, 5% CO2) in RPMI 1640 media supplemented with 10% fetal calf serum (RPMI-10) and suspended with tumor target cells or media after thrice washing with RPMI-10.
• RPMI 1640 media supplemented with 10% fetal calf serum (RPMI-10)
• the cells were stained with PE/Cy7-conjugated anti-CD56 mAb, APC/Cy7-conjugated anti-CD16 mAb, and PE-CF594–conjugated anti-CD3 mAb (BioLegend, San Diego, CA). Cells were incubated for 15 minutes at 4°C, washed, and fixed with 2% paraformaldehyde. Intracellular IFN-g was measured as an indicator of NK cell activation. Briefly, cells were exposed to permeabilization buffer (BD Biosciences, San Jose, CA) and incubated with Pacific Blue–conjugated antihuman IFN-g (BioLegend, San Diego, CA) for 20 minutes.
• permeabilization buffer BD Biosciences, San Jose, CA
• the cells were finally washed and evaluated by fluorescence-activated cell sorting analysis using a LSRII flow cytometer (BD Biosciences, San Jose, CA) gating on CD56 + CD3 – cells. Cytotoxicity potency was additionally measured in real time. Magnetic bead enriched CD56 + CD3- NK effector cells were plated into 96-well flat clear bottom polystyrene tissue- culture treated microplates (Corning, Flintshire, UK) along with red labeled tumor cells and the plates were transferred into the INCUCYTE ZOOM platform (Essen Bioscience, Inc., Ann Arbor, MI) which was housed inside a cell incubator at 37°C/5% CO 2 .
• LSRII flow cytometer BD Biosciences, San Jose, CA
• NK cell expansion via IL-15 stimulation To measure TriKE potency that depends on its functional IL-15 moiety, PBMCs or enriched NK cells from healthy donors were labeled with CELLTRACE violet proliferation dye (Invitrogen, Carlsbad, CA) according to kit specifications. After staining, effector cells were cultured with 50 nM of TriKEs or controls and incubated in a humidified atmosphere containing 5% CO 2 at 37°C for seven days.
• MMWThF maintenance therapy three times per week
• mice were injected with 100 ml of 30 mg/ml luciferin substrate for 10 minutes and then imaged under isoflurane gas sedation. Imaging data were gathered using Xenogen Ivis 100 imaging system with Living Image 2.5 software (Xenogen Corporation, Hopkington MA). Mice were weighed weekly when possible.
• Xenogen Ivis 100 imaging system with Living Image 2.5 software Xenogen Corporation, Hopkington MA. Mice were weighed weekly when possible.
• the complete disclosure of all patents, patent applications, and publications, and electronically available material including, for instance, nucleotide sequence submissions in, e.g., GenBank and RefSeq, and amino acid sequence submissions in, e.g., SwissProt, PIR, PRF, PDB, and translations from annotated coding regions in GenBank and RefSeq) cited herein are incorporated by reference in their entirety.
• each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as possible. All numerical values, however, inherently contain a range necessarily resulting from the standard deviation found in their respective testing measurements. All headings are for the convenience of the reader and should not be used to limit the meaning of the text that follows the heading, unless so specified.
• SEQ ID NO:30 Trastuzumab-based TriKE DNA sequence – E. coli codon optimized SEQ ID NO:31 – Signal peptide/Anti-HER2 light chain/linker/wtIL15/linker/cam16 SEQ ID NO:32 – Signal peptide/Anti-HER2 heavy chain SEQ ID NO:33 – Signal peptide/Anti-HER2 heavy chain Fab/T2A/signal peptide/Anti-HER2 light chain/linker/wtIL15/linker/cam16 SEQ ID NO:34 – Linker SEQ ID NO:35 – Linker SEQ ID NO:36 – Signal peptide SEQ ID NO:37 – T2A self-cleaving peptide

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