WO2021040649A3 - Dispositif de détection d'hygiène - Google Patents

Dispositif de détection d'hygiène Download PDF

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Publication number
WO2021040649A3
WO2021040649A3 PCT/TR2020/050737 TR2020050737W WO2021040649A3 WO 2021040649 A3 WO2021040649 A3 WO 2021040649A3 TR 2020050737 W TR2020050737 W TR 2020050737W WO 2021040649 A3 WO2021040649 A3 WO 2021040649A3
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
indole
reactive
detection device
tryptophan
Prior art date
Application number
PCT/TR2020/050737
Other languages
English (en)
Other versions
WO2021040649A2 (fr
Inventor
Hüsra TUNÇER
Atakan ERYILDIZ
Ahmet KOLUMAN
Original Assignee
Pamukkale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pamukkale University filed Critical Pamukkale University
Publication of WO2021040649A2 publication Critical patent/WO2021040649A2/fr
Publication of WO2021040649A3 publication Critical patent/WO2021040649A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/22—Testing for sterility conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/988—Lyases (4.), e.g. aldolases, heparinase, enolases, fumarase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Public Health (AREA)
  • Cell Biology (AREA)
  • Epidemiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un dispositif (1) de détection d'hygiène qui peut être utilisé dans les domaines tels que les aliments, l'eau, l'agriculture, la médecine, la biologie et la chimie, et qui permet de déterminer les caractéristiques toxiques provoquées par la contamination de bactéries ayant la capacité de former un indole par décomposition de l'acide aminé tryptophane. L'enzyme tryptophanase produite par E. coli ou de telles bactéries réagit avec le tryptophane, et de l'acide pyruvique, de l'ammoniac et un indole sont formés à partir du tryptophane par désamination. Dans le dispositif (1) de détection d'hygiène selon l'invention, un environnement d'incubation de 37 °C est prévu pour les bactéries, et les bactéries sont laissées incuber à 37 °C pendant 4 à 8 heures. Après réception de l'avertissement après 4 à 8 heures, 0,5 mL de réactif de Kovac est ajouté goutte à goutte sur l'indole qui est produit par une bactérie. Le réactif de Kovac est un réactif biochimique constitué d'alcool isoamylique, de paradiméthylaminobenzaldéhyde et d'acide chlorhydrique. L'indole produit par les bactéries réagit avec le paradiméthylaminobenzaldéhyde, pour former une couche colorée bordeaux à la surface du tube Eppendorf dans lequel ils sont placés. Le lecteur optique (6) détecte la fréquence de cette couleur bordeaux, et la présence de bactéries, le cas échéant, est révélée à l'utilisateur par l'intermédiaire de l'écran LCD (8).
PCT/TR2020/050737 2019-08-23 2020-08-21 Dispositif de détection d'hygiène WO2021040649A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2019/12725 2019-08-23
TR2019/12725A TR201912725A2 (tr) 2019-08-23 2019-08-23 Bi̇r hi̇jyen tespi̇t ci̇hazi

Publications (2)

Publication Number Publication Date
WO2021040649A2 WO2021040649A2 (fr) 2021-03-04
WO2021040649A3 true WO2021040649A3 (fr) 2021-06-17

Family

ID=74686129

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2020/050737 WO2021040649A2 (fr) 2019-08-23 2020-08-21 Dispositif de détection d'hygiène

Country Status (2)

Country Link
TR (1) TR201912725A2 (fr)
WO (1) WO2021040649A2 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3378346A (en) * 1965-02-05 1968-04-16 Warner Lambert Pharmaceutical Diagnostic preparation for the detection of indole
WO2008007206A2 (fr) * 2006-07-11 2008-01-17 Universita' Degli Studi Roma Tre Procédé colorimétrique et dispositive associé de detection de charge bactérienne
US20100062467A1 (en) * 2007-02-08 2010-03-11 Biomerieux Medium For Detecting And/Or Identifying Bacteria

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3378346A (en) * 1965-02-05 1968-04-16 Warner Lambert Pharmaceutical Diagnostic preparation for the detection of indole
WO2008007206A2 (fr) * 2006-07-11 2008-01-17 Universita' Degli Studi Roma Tre Procédé colorimétrique et dispositive associé de detection de charge bactérienne
US20100062467A1 (en) * 2007-02-08 2010-03-11 Biomerieux Medium For Detecting And/Or Identifying Bacteria

Also Published As

Publication number Publication date
WO2021040649A2 (fr) 2021-03-04
TR201912725A2 (tr) 2021-03-22

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