TR201912725A2 - Bi̇r hi̇jyen tespi̇t ci̇hazi - Google Patents
Bi̇r hi̇jyen tespi̇t ci̇haziInfo
- Publication number
- TR201912725A2 TR201912725A2 TR2019/12725A TR201912725A TR201912725A2 TR 201912725 A2 TR201912725 A2 TR 201912725A2 TR 2019/12725 A TR2019/12725 A TR 2019/12725A TR 201912725 A TR201912725 A TR 201912725A TR 201912725 A2 TR201912725 A2 TR 201912725A2
- Authority
- TR
- Turkey
- Prior art keywords
- bacteria
- bacterium
- indole
- tryptophan
- detection device
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- 241000894006 Bacteria Species 0.000 abstract 8
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 abstract 6
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 abstract 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 abstract 3
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 abstract 3
- FZRCKLPSHGTOAU-UHFFFAOYSA-N 6-amino-1,4-dimethylcyclohexa-2,4-diene-1-carbaldehyde Chemical compound CC1=CC(N)C(C)(C=O)C=C1 FZRCKLPSHGTOAU-UHFFFAOYSA-N 0.000 abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 abstract 2
- 241000167854 Bourreria succulenta Species 0.000 abstract 1
- 208000034656 Contusions Diseases 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 102100040653 Tryptophan 2,3-dioxygenase Human genes 0.000 abstract 1
- 101710136122 Tryptophan 2,3-dioxygenase Proteins 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- -1 agriculture Substances 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 229910021529 ammonia Inorganic materials 0.000 abstract 1
- 208000034526 bruise Diseases 0.000 abstract 1
- 235000019693 cherries Nutrition 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 230000009615 deamination Effects 0.000 abstract 1
- 238000006481 deamination reaction Methods 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 150000002475 indoles Chemical class 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/22—Testing for sterility conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/988—Lyases (4.), e.g. aldolases, heparinase, enolases, fumarase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Public Health (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Epidemiology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Bu buluş gıda, su, tarım, tıp, biyoloji, kimya ve bunun gibi alanlarda kullanılan ve triptofan aminoasidini ayrıştırarak indol meydana getirme yeteneğine sahip bakterilerin kontaminasyonundan kaynaklanan toksik niteliklerin belirlenmesini sağlayan bir hijyen tespit cihazı (1) ile ilgildiir. E. Coli ve bunun gibi bir bakterinin ürettiği triptofanaz enzimi ile triptofan etkileşime girmekte ve deaminasyon ile triptofandan purivik asit, amonyak ve indol meydana gelmektedir. Buluş konusu hijyen tespit cihazında (1) öncelikle bakteriye 37 derecelik bir etüv ortamı sağlanmakta ve 4-8 saat boyunca bakteri 37 derecede inkübasyona bırakılmaktadır. 4-8 saat sonunda gelen uyarı ile bakteri tarafından yeterli miktarda oluşturulan indol üzerine 0.5 ml Kovaks reaktifi damlatılmaktadır. Kovaks reaktifi izoamil alkol, paradimetilaminobenzaldehit ve hidroclorik asitten oluşan biyokimyasal bir reaktiftir. Bakteri bakteri tarafından üretilen indol para-dimetilaminobenzaldehit ile etkileşmekte ve bulundukları ependorf yüzeyinde vişne çürüğü renginde bir tabaka oluşturmaktadır. Optik okuyucu (6) bu vişne çürüğü renginin frekansı algılamakta ve varsa bakteri varlığı LCD ekranda (8) kullanıcıya aktarılmaktadır.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2019/12725A TR201912725A2 (tr) | 2019-08-23 | 2019-08-23 | Bi̇r hi̇jyen tespi̇t ci̇hazi |
| PCT/TR2020/050737 WO2021040649A2 (en) | 2019-08-23 | 2020-08-21 | A hygiene detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2019/12725A TR201912725A2 (tr) | 2019-08-23 | 2019-08-23 | Bi̇r hi̇jyen tespi̇t ci̇hazi |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TR201912725A2 true TR201912725A2 (tr) | 2021-03-22 |
Family
ID=74686129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TR2019/12725A TR201912725A2 (tr) | 2019-08-23 | 2019-08-23 | Bi̇r hi̇jyen tespi̇t ci̇hazi |
Country Status (2)
| Country | Link |
|---|---|
| TR (1) | TR201912725A2 (tr) |
| WO (1) | WO2021040649A2 (tr) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3378346A (en) * | 1965-02-05 | 1968-04-16 | Warner Lambert Pharmaceutical | Diagnostic preparation for the detection of indole |
| ITBO20060531A1 (it) * | 2006-07-11 | 2008-01-12 | Univ Degli Studi Roma Tre | Metodo colorimetrico e relativo dispositivo per la rilevazione della carica batterica |
| FR2912425B1 (fr) * | 2007-02-08 | 2012-08-31 | Biomerieux Sa | Milieu de detection et/ou d'identification de bacteries |
-
2019
- 2019-08-23 TR TR2019/12725A patent/TR201912725A2/tr unknown
-
2020
- 2020-08-21 WO PCT/TR2020/050737 patent/WO2021040649A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021040649A2 (en) | 2021-03-04 |
| WO2021040649A3 (en) | 2021-06-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wong et al. | Real time detection of live microbes using a highly sensitive bioluminescent nitroreductase probe | |
| DeQueiroz et al. | Antimicrobial activity and effectiveness of a combination of sodium hypochlorite and hydrogen peroxide in killing and removing Pseudomonas aeruginosa biofilms from surfaces | |
| Sanmartín et al. | Color measurements as a reliable method for estimating chlorophyll degradation to phaeopigments | |
| Chenia et al. | Characterization of virulence, cell surface characteristics and biofilm‐forming ability of Aeromonas spp. isolates from fish and sea water | |
| US20030162164A1 (en) | Comparative phenotype analysis of cells, including testing of biologically active compounds | |
| AU2018321580B2 (en) | BRET sensor molecules for detecting hydrolases | |
| Alasmar et al. | Isolation of a novel Kluyveromyces marxianus strain QKM-4 and evidence of its volatilome production and binding potentialities in the biocontrol of toxigenic fungi and their mycotoxins | |
| Bhagat et al. | Screening of Mesorhizobium spp. for control of Fusarium wilt in chickpea in vitro conditions | |
| WO2013084772A1 (ja) | 細胞の測定方法及び細胞の測定用試薬 | |
| TR201912725A2 (tr) | Bi̇r hi̇jyen tespi̇t ci̇hazi | |
| Nguyen-Ngoc et al. | Synchronous-scan fluorescence of algal cells for toxicity assessment of heavy metals and herbicides | |
| Fakhruzzaman et al. | Study on in vivo effects of bacterial histidine kinase inhibitor, Waldiomycin, in Bacillus subtilis and Staphylococcus aureus | |
| Helmerhorst et al. | Experimental strategy to discover microbes with gluten-degrading enzyme activities | |
| Zhernov et al. | Molecular mechanisms of scombroid food poisoning | |
| Camara et al. | Inhibition of p-aminobenzoate and folate syntheses in plants and apicomplexan parasites by natural product rubreserine | |
| WO2008104680A3 (fr) | Milieu de détection et/ou d'identification de bactéries | |
| Chen et al. | Assessing the effects of polychlorinated biphenyls (Aroclor 1254) on a scleractinian coral (Stylophora pistillata) at organism, physiological, and molecular levels | |
| WO2006096710A3 (en) | Detection, imaging, and depletion of chlamydia | |
| WO2005067425A3 (en) | Bacterial biosensors | |
| Tantimongcolwat et al. | Polyacrylamide hydrogel encapsulated E. coli expressing metal-sensing green fluorescent protein as a potential tool for copper ion determination | |
| Rodríguez | Understanding nitrile-degrading enzymes: classification, biocatalytic nature and current applications | |
| İzgördü et al. | Understanding the transition to viable but non-culturable state in Escherichia coli W3110: a comprehensive analysis of potential spectrochemical biomarkers | |
| KR101802422B1 (ko) | 펩티도글리칸 결합단백질을 이용한 박테리아 검출용 프로브 및 그의 이용 | |
| Wu et al. | μMET: A Novel Reusable Microfluidic Chip for Precision Microbial Enumeration Tests | |
| EP3071027A1 (en) | Assay-ready frozen cell and method for minimizing variability in the performance thereof |