WO2021031680A1 - Dispositif d'inoculation stérile par fermentation - Google Patents

Dispositif d'inoculation stérile par fermentation Download PDF

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Publication number
WO2021031680A1
WO2021031680A1 PCT/CN2020/097197 CN2020097197W WO2021031680A1 WO 2021031680 A1 WO2021031680 A1 WO 2021031680A1 CN 2020097197 W CN2020097197 W CN 2020097197W WO 2021031680 A1 WO2021031680 A1 WO 2021031680A1
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WO
WIPO (PCT)
Prior art keywords
fermentation
side door
box body
tank
ring body
Prior art date
Application number
PCT/CN2020/097197
Other languages
English (en)
Chinese (zh)
Inventor
朱玲丽
刘祯祥
付成丽
郑斌丽
盛琛来
阿丽雅
夏青青
项晶
赵丙辉
陈雯
王卫军
Original Assignee
浙江李子园食品股份有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 浙江李子园食品股份有限公司 filed Critical 浙江李子园食品股份有限公司
Publication of WO2021031680A1 publication Critical patent/WO2021031680A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler

Definitions

  • the utility model belongs to the technical field of food processing, and specifically relates to a fermentation aseptic inoculation device.
  • This utility model is to address the above-mentioned problems in the existing fermented milk production process, and propose a sterile environment that can pass through a small range, which can greatly reduce the fermentation of materials infected by bacteria during the inoculation of fermented products. Bacteria inoculation device.
  • the fermentation aseptic inoculation device is characterized in that it includes a fermentation tank and an aseptic inoculation bin.
  • the fermentation tank includes a tank body and a stuffy cover arranged on the upper part of the tank.
  • the aseptic inoculation bin includes a tank body and a set On the side door on the side of the box body, the box body is arranged on the upper part of the tank body, and the upper part of the tank body is connected with the inner cavity of the box body through a feeding pipe.
  • the side door can be tightly buckled with the box body.
  • the two hand-reaching holes are also provided with hand-reaching hole sealing devices.
  • one side of the box body is provided with an opening, the opening is provided with a first convex edge, the side door is provided at the opening, the lower part of the side door is hinged with the bottom of the box body, and the side door facing the opening is provided with
  • the first convex edge can be locked into the groove. After the first convex edge is locked into the groove, the side door is tightly buckled with the box body.
  • a ring-shaped body is provided at the hand-extending hole, the ring-shaped body is provided with a cylindrical hole having the same size as the hand-extending hole, the cylindrical hole is butted with the hand-extending hole, and the ring body includes steps arranged in a stepped shape.
  • the first ring body and the second ring body, the second ring body is fixedly connected with the box body, and the sealing device is arranged on the first ring body.
  • the sealing device includes two covers corresponding to the above-mentioned ring bodies one-to-one, the cover includes a cover body and a locking structure, and the cover body is arranged on a side facing the first ring body There is a circle of limiting grooves, the outer end of the first ring body can be clamped into the limiting grooves, and the locking structure is arranged between the cover body and the first ring body to lock the cover body and the first ring body.
  • the outer end of the first ring body is provided with a second convex edge
  • the height of the second convex edge is equivalent to the width of the above-mentioned limiting groove
  • the thickness of the second convex edge is equivalent to the depth of the above-mentioned limiting groove.
  • the locking structure includes a hoop and a locking member
  • the locking member includes a locking screw
  • the hoop includes two elastic semicircles
  • one end of the two elastic semicircles is hinged
  • the two elastic semicircles The other end of the body is provided with a lug
  • the locking screw is located in the two lugs
  • one end of the locking screw is fixedly connected with one of the lugs
  • the other end is provided with a locking nut
  • the inner side of the elastic semicircular body is provided with a slot
  • the card slot can be clamped on the cover edge of the cover body, and the second convex edge of the first ring body is also located in the card slot.
  • the second convex edge of the first ring body and the cover edge of the cover body are both located in the slot, so when the two elastic semicircular bodies are locked by the locking member, the cover body can cover the ring body so that the box body is in Closed state.
  • the upper part of the feed pipe is provided with a stuffy cover. Open the side door first, and put the alcohol spray can and bacteria (fermentation bag) for disinfection into the inner cavity of the box. Human hands reach into the box through the two hand holes and use alcohol The watering can disinfects the inner cavity area and human hands of the box. After disinfection, open the clogged cover and put the bacteria into the tank of the fermentation tank. After the bacteria are planted, the clogged cover is put back, so that the tank is in a sealed state and the entire The inoculation process.
  • the hand hole can be sealed with a cover to prevent dust from falling into the box.
  • the box body is a stainless steel box body.
  • the side door is a transparent acrylic side door.
  • the utility model is equipped with a sterile inoculation bin with a side door, two hand holes and a sealing device.
  • a sterile inoculation bin with a side door, two hand holes and a sealing device.
  • Figure 1 is a schematic diagram of the structure of the utility model
  • Figure 2 is a schematic diagram of the structure of the aseptic inoculation bin of the present invention.
  • Figure 3 is a schematic diagram of part of the structure of the aseptic inoculation bin of the present invention.
  • Figure 4 is a schematic structural view of the sterile vaccination chamber of the present invention in another direction;
  • Figure 5 is a schematic diagram of part of the structure of the aseptic inoculation bin of the present invention.
  • Figure 6 is a schematic diagram of the structure of the cover of the present invention.
  • the fermentation aseptic inoculation device of the present invention includes a fermentation tank 1 and an aseptic inoculation bin 2.
  • the fermentation tank 1 includes a tank body 3 and a blind cover 4 arranged on the upper part of the tank body 3.
  • the aseptic inoculation bin 2 includes a box body 5 and a side door 6 arranged on one side of the box body 5, and the box body 5 is arranged on the tank body.
  • the upper part of the tank 3, the upper part of the tank body 3 is communicated with the inner cavity of the box body 5 through a feeding tube, the blanking cover 4 is set on the end of the feeding tube located in the box body 5, and the side door 6 is provided with two hand-holding With the extending hand hole 7, the side door 6 can be tightly buckled with the box body 5.
  • the two hand extending holes 7 are also provided with hand hole 7 sealing devices.
  • One side of the box body 5 is provided with an opening 8, and the opening 8 is provided with a first convex edge 9.
  • the above-mentioned side door 6 is provided at the opening 8, and the lower part of the side door 6 is hinged with the bottom of the box body 5 (as shown in Figure 1, hinged
  • the structure is the prior art and will not be described in detail here).
  • the side door 6 facing the opening 8 is provided with a groove 10 adapted to the first protrusion 9.
  • the first protrusion 9 can be locked into the groove 10.
  • the side door 6 is tightly buckled with the box body 5.
  • An annular body 11 is provided at the hand-reaching hole 7.
  • the annular body 11 is provided with a cylindrical hole 12 having the same size as the hand-reaching hole 7.
  • the cylindrical hole 12 is butted with the hand-reaching hole 7, and the annular body 11 includes a stepped row
  • the first ring body 13 and the second ring body 14 of the cloth, the second ring body 14 and the box body 5 are fixedly connected, and the sealing device is arranged on the first ring body 13.
  • the sealing device includes two covers corresponding to the above-mentioned ring body 11 one-to-one.
  • the cover includes a cover body 15 and a locking structure.
  • the cover body 15 is provided with a ring on the side facing the first ring body 13.
  • the limiting groove 16, the outer end of the first ring body 13 can be clamped into the limiting groove 16, the locking structure is arranged between the cover body 15 and the first ring body 13 to lock the cover body 15 and the first ring body 13 .
  • the outer end of the first ring body 13 is provided with a second protruding edge 17, the height of the second protruding edge 17 is equivalent to the width of the aforementioned limiting groove 16, and the thickness of the second protruding edge 17 is equivalent to the depth of the aforementioned limiting slot 16 , The second convex edge 17 can be locked into the limiting slot 16.
  • the locking structure includes a hoop 18 and a locking member.
  • the locking member includes a locking screw 19.
  • the hoop 18 includes two elastic semicircles 20, one end of the two elastic semicircles 20 is hinged, and the two elastic semicircles The other end of the 20 is provided with a hoop 21, the locking screw 19 is located in the two hoop ears 21, the hoop 21 is U-shaped, one end of the locking screw 19 and the shaft of one of the hoop ears 21 (horizontally arranged on the hoop ears 21) 21) is fixedly connected, the other end is provided with a lock nut 22, the inner side of the elastic semicircular body 20 is provided with a slot 23, the slot 23 can be locked on the edge of the cover 15 and make the first ring 13 The two convex edges 17 are also located in the slot 23.
  • the second convex edge 17 of the first ring body 13 and the cover edge of the cover 15 are both located in the groove 23, so when the two elastic semicircular bodies 20 are locked by the locking member, the cover 15 can cover the ring
  • the body 11 makes the box body 5 in a closed state.
  • the upper part of the feed pipe is provided with a stuffy cover 4. Open the side door 6 first, and put the alcohol watering can and bacteria (fermentation bag) for disinfection into the inner cavity of the box body 5.
  • the two hands of the person extend into the box through the two hand holes 7 In the body 5, use an alcohol spray can to disinfect the inner cavity area of the box body 5 and human hands.
  • the plug 4 put the bacteria into the tank 3 of the fermentation tank 1.
  • the plug 4 Put the lid back to make the tank 3 in a sealed state, completing the entire inoculation process.
  • a hole can be opened on the box body 5 for the pipe of the spray washing device to pass through.
  • the hand hole 7 can be sealed by a cover to prevent dust from falling into the box body 5.
  • the box body 5 is a stainless steel box body 5.
  • the side door 6 is a transparent acrylic side door 6.
  • the utility model is provided with a sterile inoculation bin 2 including a side door 6, two hand-out holes 7 and a sealing device.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Dispositif d'inoculation stérile par fermentation Le dispositif d'inoculation stérile par fermentation résout les problèmes suivants: dans un procédé de production de lait fermenté existant, les matériaux et les souches dans un réservoir sont en contact direct avec l'extérieur dans un processus de communication et sont facilement pollués par des bactéries extérieures , le rendu qu'un produit fermenté est non qualifié, etc. Le dispositif d'inoculation stérile par fermentation comprend un réservoir de fermentation et un bac d'inoculation stérile. Le réservoir de fermentation comprend un corps de réservoir et un capuchon d'ébauche disposé sur la partie supérieure du corps de réservoir ; le bac d'inoculation stérile comprend un corps de boîte et une porte latérale disposée sur un côté du corps de boîte, le corps de boîte est disposé sur la partie supérieure du corps de réservoir, la partie supérieure du corps de réservoir est en communication avec une cavité interne du corps de boîte au moyen d'un tuyau d'alimentation, deux trous d'étirement de la main permettant aux mains de s'étirer dans la porte latérale, la porte latérale peut être bouclée avec le corps de boîte dans un mode étanche, et des dispositifs d'étanchéité des trous d'étirage de main sont en outre disposés au niveau des deux trous d'étirement de main. Le dispositif d'inoculation stérile par fermentation est pourvu du bac d'inoculation stérile comprenant la porte latérale, les deux trous d'étirement de la main, et les dispositifs d'étanchéité, et par conséquent, le risque de sécurité que des matériaux soient infectés par des microbes infectieux dans le processus d'inoculation de produits fermentés est considérablement réduit en utilisant un environnement stérile de à petite échelle.
PCT/CN2020/097197 2019-08-21 2020-06-19 Dispositif d'inoculation stérile par fermentation WO2021031680A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201921385989.7U CN210481363U (zh) 2019-08-21 2019-08-21 发酵无菌接种装置
CN201921385989.7 2019-08-21

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Publication Number Publication Date
WO2021031680A1 true WO2021031680A1 (fr) 2021-02-25

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PCT/CN2020/097197 WO2021031680A1 (fr) 2019-08-21 2020-06-19 Dispositif d'inoculation stérile par fermentation

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CN (1) CN210481363U (fr)
WO (1) WO2021031680A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113832011A (zh) * 2021-09-22 2021-12-24 川北医学院 一种减少其他菌种混入的细菌溶液接种装置

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN210481363U (zh) * 2019-08-21 2020-05-08 浙江李子园食品股份有限公司 发酵无菌接种装置
CN111893030B (zh) * 2020-08-10 2023-09-08 惠农达生物技术(江苏)有限公司 一种固态发酵液体菌种接种装置
CN115736013B (zh) * 2022-12-06 2023-10-20 江苏汉肽生物医药有限公司 一种乳制品发酵罐菌种无污染添加装置

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2786130Y (zh) * 2004-11-17 2006-06-07 邹书海 开放式超净箱
CN202022936U (zh) * 2011-03-24 2011-11-02 牡丹江佰佳信生物科技有限公司 一种封闭式发酵罐接种系统
CN202246688U (zh) * 2011-04-27 2012-05-30 安徽省皖北药业股份有限公司 一种发酵罐接种装置
CN203653554U (zh) * 2013-12-25 2014-06-18 潍坊欧普诺生物科技有限公司 一种发酵罐接种装置
CN207420694U (zh) * 2017-11-15 2018-05-29 广州汽车集团股份有限公司 卡箍总成
CN208980712U (zh) * 2018-06-22 2019-06-14 广州市朴诚乳业有限公司 发酵待装系统
CN210481363U (zh) * 2019-08-21 2020-05-08 浙江李子园食品股份有限公司 发酵无菌接种装置

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2786130Y (zh) * 2004-11-17 2006-06-07 邹书海 开放式超净箱
CN202022936U (zh) * 2011-03-24 2011-11-02 牡丹江佰佳信生物科技有限公司 一种封闭式发酵罐接种系统
CN202246688U (zh) * 2011-04-27 2012-05-30 安徽省皖北药业股份有限公司 一种发酵罐接种装置
CN203653554U (zh) * 2013-12-25 2014-06-18 潍坊欧普诺生物科技有限公司 一种发酵罐接种装置
CN207420694U (zh) * 2017-11-15 2018-05-29 广州汽车集团股份有限公司 卡箍总成
CN208980712U (zh) * 2018-06-22 2019-06-14 广州市朴诚乳业有限公司 发酵待装系统
CN210481363U (zh) * 2019-08-21 2020-05-08 浙江李子园食品股份有限公司 发酵无菌接种装置

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113832011A (zh) * 2021-09-22 2021-12-24 川北医学院 一种减少其他菌种混入的细菌溶液接种装置

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