WO2021025649A2 - Production of mono-, diglyceride by the method of enzymatic glycerolysis from waste bone fat in renderin (animal waste product enterprise) plants - Google Patents
Production of mono-, diglyceride by the method of enzymatic glycerolysis from waste bone fat in renderin (animal waste product enterprise) plants Download PDFInfo
- Publication number
- WO2021025649A2 WO2021025649A2 PCT/TR2020/050664 TR2020050664W WO2021025649A2 WO 2021025649 A2 WO2021025649 A2 WO 2021025649A2 TR 2020050664 W TR2020050664 W TR 2020050664W WO 2021025649 A2 WO2021025649 A2 WO 2021025649A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mono
- diglyceride
- fat
- waste
- production
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
- C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
- C11C3/04—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils
- C11C3/06—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fats or fatty oils with glycerol
Definitions
- the invention is related to a method of producing mono-, diglycerides from waste bone fat in rendering plants to be used in the food sector.
- Emulsifiers are substances that allow the foods to have a finely dispersed structure by reducing surface tension; and they ensure stability by reducing the free energy on the surface between two phases and forming an adsorbed film around the droplets that form the discontinuous phase. With their positive effects including those that prevent physical defects that may be resulting from the extended shelf lives of foods as well as rheological, textural and sensory properties, emulsifiers are a group of food additives commonly used today. Mono- and diglycerides of fatty acids are in this group. Commonly used in the food sector to ensure stability in products in which fat and water phases are mixed, mono-, diglycerides are generally produced from vegetable oils.
- mono-, diglycerides are used in the production of margarine and ice cream to ensure emulsion; in meat products such as sausages and salamis to prevent separation of oil; in bakery products to increase overrun and prevent starch retrogradation; finally in products such as spices and instant soups to ensure solubility .
- Mono-, diglycerides are not only used in the food sector but also very common in drug formulations in the field of pharmaceutics as well as cosmetics and chemical industry in order to create the desired form.
- the method of producing mono-, diglyceride food emulsifiers from vegetable and animal sources is comprised in the state of the art.
- the difference between the subject of the invention and the state of the art is the production of mono-, diglycerides from waste bone fat from rendering plants. Thanks to this technical difference, unexpected effects were observed in the properties of the food emulsifier.
- the covered emulsifiers have been found to have superior in comparison to the mono-, diglycerides commercially available in the market in terms of their rheological properties and the way they create an emulsion.
- the emulsifier samples produced were tested on the commercially available model products and the comparison with their counterparts revealed its superiority in terms of structure. They were studied on milk ice (ice-cream) samples.
- the invention is related to the production of mono-, diglycerides by the method of enzymatic glycerolysis from waste bone fat from Rendering plants
- Lipase Novartis 405 was used as enzyme for the production of liquid mono-, diglycerides.
- the reaction was formed by mixing glycerol (15% based on the fat content), enzyme (10% based on the fat content) and rendering plant waste bone fat and the reaction was carried out in closed glass bottles in the agitation water bath for 5 hours at 70°C. The reaction can occur in 2 to 8 hours of agitation water bath at a temperature of 50-80 °C.
- the product properties of the mono-, diglyceride emulsifier that is produced were found to be such that; peroxide value was in the range of 22-23 meq O2 / kg, p-anisidin, which represents the oxidation products, was in the range of 1.5-2, K-232, which represents the primary oxidation products, were in the range of 5-6, K-270, which represents the secondary oxidation products were in the range of 0.4-0.7 and HLD, which is the indication of the hydrophilic-lipophilic balance were in the range of 10-13.
- the rheological assessment of the emulsion containing 1% emulsifier produced with mono-, diglyceride emulsifier showed that the viscosity was in the range of 75-90 mPa.s.
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- Chemical & Material Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention is related to the method of producing mono-, diglycerides by the method of enzymatic glycerolysis from waste bone fat from Rendering plants.
Description
PRODUCTION OF MONO-, DIGLYCERIDE BY THE METHOD OF ENZYMATIC GLYCEROLYSIS FROM WASTE BONE FAT IN RENDERING (ANIMAL WASTE PRODUCT ENTERPRISE) PLANTS
TECHNICAL FIELD
The invention is related to a method of producing mono-, diglycerides from waste bone fat in rendering plants to be used in the food sector.
PRIOR ART
Emulsifiers are substances that allow the foods to have a finely dispersed structure by reducing surface tension; and they ensure stability by reducing the free energy on the surface between two phases and forming an adsorbed film around the droplets that form the discontinuous phase. With their positive effects including those that prevent physical defects that may be resulting from the extended shelf lives of foods as well as rheological, textural and sensory properties, emulsifiers are a group of food additives commonly used today. Mono- and diglycerides of fatty acids are in this group. Commonly used in the food sector to ensure stability in products in which fat and water phases are mixed, mono-, diglycerides are generally produced from vegetable oils.
Estimated to be used at a rate of approximately 500 tonnes a month in Turkey, mono-, diglycerides are used in the production of margarine and ice cream to ensure emulsion; in meat products such as sausages and salamis to prevent separation of oil; in bakery products to increase overrun and prevent starch retrogradation; finally in products such as spices and instant soups to ensure solubility .Mono-, diglycerides are not only used in the food sector but also very common in drug formulations in the field of pharmaceutics as well as cosmetics and chemical industry in order to create the desired form.
These emulsifiers are imported and not produced in the country. Producing companies extract these mono-, diglycerides from vegetable oils and animal fat.
Mono-, diglyceride production has been globally carried out using enzymatic and chemical techniques for many years. However, the state of the art does not comprise the production
of mono-, diglycerides from waste fat of rendering plants by means of enzymatic glycerolysis.
BRIEF DESCRIPTION OF THE INVENTION
The method of producing mono-, diglyceride food emulsifiers from vegetable and animal sources is comprised in the state of the art. The difference between the subject of the invention and the state of the art is the production of mono-, diglycerides from waste bone fat from rendering plants. Thanks to this technical difference, unexpected effects were observed in the properties of the food emulsifier. The covered emulsifiers have been found to have superior in comparison to the mono-, diglycerides commercially available in the market in terms of their rheological properties and the way they create an emulsion. When the emulsion samples were compared in the rheological analyses, it was found that while the viscosity of the samples prepared using the commercially available emulsifiers were 10-15 m.Pa.s on average, that of the emulsions prepared using the emulsifier extracted from the waste fat from the rendering plant was in the range of 73-75 m.Pas.This indicates that the covered emulsifiers have structures of higher consistency and the desired fluidity can be achieved in the product by using lower quantities of additives.
The emulsifier samples produced were tested on the commercially available model products and the comparison with their counterparts revealed its superiority in terms of structure. They were studied on milk ice (ice-cream) samples.
DETAILED DESCRIPTION OF THE INVENTION
The invention is related to the production of mono-, diglycerides by the method of enzymatic glycerolysis from waste bone fat from Rendering plants Lipase (Novozym 435) was used as enzyme for the production of liquid mono-, diglycerides. The reaction was formed by mixing glycerol (15% based on the fat content), enzyme (10% based on the fat content) and rendering plant waste bone fat and the reaction was carried out in closed glass bottles in the agitation water bath for 5 hours at 70°C. The reaction can occur in 2 to 8 hours of agitation water bath at a temperature of 50-80 °C. During the reaction in the ultrasonic water bath, ultrasound was applied (for 60 minutes at a frequency of 40 kHz) and acetone was added as solvent (in the fat/solvent ratio of 1:1). The ultrasonic bath application helps increase the enzyme activity in the enzymatic glycerolysis reaction,
therefore increasing interaction between the enzyme and its substrate, fat, achieving a more efficient conversion. This conversion refers to the conversion of the rendering waste fat in the form of triglyceride into mono and diglyceride. This reaction resulted in a 90% increase in product yield. The enzyme was removed through filtration, washed with isopropyl alcohol and stored at a temperature of approximately -18 °C to be used again. The remnants of the enzyme removal process were analyzed and found to be mono-, diglyceride (containing 50% monoglyceride, 40% diglyceride and 10% triglyceride).
The product properties of the mono-, diglyceride emulsifier that is produced were found to be such that; peroxide value was in the range of 22-23 meq O2 / kg, p-anisidin, which represents the oxidation products, was in the range of 1.5-2, K-232, which represents the primary oxidation products, were in the range of 5-6, K-270, which represents the secondary oxidation products were in the range of 0.4-0.7 and HLD, which is the indication of the hydrophilic-lipophilic balance were in the range of 10-13. The rheological assessment of the emulsion containing 1% emulsifier produced with mono-, diglyceride emulsifier showed that the viscosity was in the range of 75-90 mPa.s.
Claims
1. A method of producing mono-, diglyceride from waste bone fat characterized by the following steps of operation; · Adding 15% glycerol based on the fat content, 10% enzyme based on the fat content along with rendering plant waste fat to the reaction environment,
• Leaving the liquid form of mono-, diglyceride in the agitation water bath for 2-8 hours at 50-80°C.
2. The method of producing mono-, diglyceride according to claim 1, characterized by comprising the step of ultrasound application for 60 minutes at a frequency of 40 kHz to the environment in an ultrasonic water bath during the reaction.
3. The method of producing mono-, diglyceride according to claim 1, characterized in that the solution comprises acetone as solvent at a ratio of 1:1 (fat/solvent).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TR2019/11727 | 2019-08-02 | ||
| TR2019/11727A TR201911727A2 (en) | 2019-08-02 | 2019-08-02 | RENDERING (ANIMAL BY-PRODUCT FACILITY) FACILITY PRODUCTION OF MONO-, DIGLYCERIDE FROM BONE WASTE OIL WITH ENZYMATIC GLYCEROLYSIS METHOD |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2021025649A2 true WO2021025649A2 (en) | 2021-02-11 |
| WO2021025649A3 WO2021025649A3 (en) | 2021-10-14 |
Family
ID=74504088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/TR2020/050664 WO2021025649A2 (en) | 2019-08-02 | 2020-07-28 | Production of mono-, diglyceride by the method of enzymatic glycerolysis from waste bone fat in renderin (animal waste product enterprise) plants |
Country Status (2)
| Country | Link |
|---|---|
| TR (1) | TR201911727A2 (en) |
| WO (1) | WO2021025649A2 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6225987A (en) * | 1985-07-25 | 1987-02-03 | Meito Sangyo Kk | Production of diglyceride by enzymatic method |
| IT1244401B (en) * | 1990-04-03 | 1994-07-11 | Mario Menegatto | Process and plant for obtaining fats, hydrolysed protein concentrates and bone residue with very low fat content, from the by-products of slaughtering such as bone, fat and pork fat. All these products can be utilized by the food and/or livestock industry. |
| CN106148439A (en) * | 2016-09-08 | 2016-11-23 | 东北农业大学 | A kind of method that power ultrasonic pretreatment enzyme law catalysis Adeps Sus domestica glycerol rhizolomy prepares diglyceride |
-
2019
- 2019-08-02 TR TR2019/11727A patent/TR201911727A2/en unknown
-
2020
- 2020-07-28 WO PCT/TR2020/050664 patent/WO2021025649A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| TR201911727A2 (en) | 2021-02-22 |
| WO2021025649A3 (en) | 2021-10-14 |
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