WO2021018783A1 - Modified filamentous fungal host cell - Google Patents

Modified filamentous fungal host cell Download PDF

Info

Publication number
WO2021018783A1
WO2021018783A1 PCT/EP2020/071004 EP2020071004W WO2021018783A1 WO 2021018783 A1 WO2021018783 A1 WO 2021018783A1 EP 2020071004 W EP2020071004 W EP 2020071004W WO 2021018783 A1 WO2021018783 A1 WO 2021018783A1
Authority
WO
WIPO (PCT)
Prior art keywords
aspergillus
seq
gene
host cell
spo14
Prior art date
Application number
PCT/EP2020/071004
Other languages
English (en)
French (fr)
Inventor
Hiroaki Udagawa
Original Assignee
Novozymes A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes A/S filed Critical Novozymes A/S
Priority to EP20743153.7A priority Critical patent/EP4004187A1/en
Priority to CN202080049863.1A priority patent/CN114555777A/zh
Priority to US17/630,476 priority patent/US20220251581A1/en
Publication of WO2021018783A1 publication Critical patent/WO2021018783A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/04—Phosphoric diester hydrolases (3.1.4)
    • C12Y301/04004—Phospholipase D (3.1.4.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/685—Aspergillus niger

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PCT/EP2020/071004 2019-07-26 2020-07-24 Modified filamentous fungal host cell WO2021018783A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP20743153.7A EP4004187A1 (en) 2019-07-26 2020-07-24 Modified filamentous fungal host cell
CN202080049863.1A CN114555777A (zh) 2019-07-26 2020-07-24 经修饰的丝状真菌宿主细胞
US17/630,476 US20220251581A1 (en) 2019-07-26 2020-07-24 Modified filamentous fungal host cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19188683.7 2019-07-26
EP19188683 2019-07-26

Publications (1)

Publication Number Publication Date
WO2021018783A1 true WO2021018783A1 (en) 2021-02-04

Family

ID=67439129

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2020/071004 WO2021018783A1 (en) 2019-07-26 2020-07-24 Modified filamentous fungal host cell

Country Status (4)

Country Link
US (1) US20220251581A1 (zh)
EP (1) EP4004187A1 (zh)
CN (1) CN114555777A (zh)
WO (1) WO2021018783A1 (zh)

Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0238023A2 (en) 1986-03-17 1987-09-23 Novo Nordisk A/S Process for the production of protein products in Aspergillus oryzae and a promoter for use in Aspergillus
WO1990000192A1 (en) 1988-07-01 1990-01-11 Genencor, Inc. Aspartic proteinase deficient filamentous fungi
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
WO1995033836A1 (en) 1994-06-03 1995-12-14 Novo Nordisk Biotech, Inc. Phosphonyldipeptides useful in the treatment of cardiovascular diseases
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US6011147A (en) 1986-04-30 2000-01-04 Rohm Enzyme Finland Oy Fungal promoters active in the presence of glucose
WO2000024883A1 (en) 1998-10-26 2000-05-04 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
WO2000056900A2 (en) 1999-03-22 2000-09-28 Novo Nordisk Biotech, Inc. Promoter sequences derived from fusarium venenatum and uses thereof
US6489127B1 (en) 2000-01-14 2002-12-03 Exelixis, Inc. Methods for identifying anti-cancer drug targets
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6511824B1 (en) 1999-03-17 2003-01-28 Exelixis, Inc. Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use
US6515109B1 (en) 2000-10-12 2003-02-04 Exelixis, Inc. Human ECT2 polypeptide
WO2004090155A2 (en) * 2003-03-31 2004-10-21 Novozymes Inc. Methods for producing biological substances in enzyme-deficient mutants of aspergillus niger
WO2010039889A2 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell
WO2012160093A1 (en) 2011-05-23 2012-11-29 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
WO2017182442A1 (en) * 2016-04-19 2017-10-26 Novozymes A/S Rlma-inactivated filamentous fungal host cell

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
EP0238023A2 (en) 1986-03-17 1987-09-23 Novo Nordisk A/S Process for the production of protein products in Aspergillus oryzae and a promoter for use in Aspergillus
US6011147A (en) 1986-04-30 2000-01-04 Rohm Enzyme Finland Oy Fungal promoters active in the presence of glucose
WO1990000192A1 (en) 1988-07-01 1990-01-11 Genencor, Inc. Aspartic proteinase deficient filamentous fungi
WO1995033836A1 (en) 1994-06-03 1995-12-14 Novo Nordisk Biotech, Inc. Phosphonyldipeptides useful in the treatment of cardiovascular diseases
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
WO2000024883A1 (en) 1998-10-26 2000-05-04 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
US6511824B1 (en) 1999-03-17 2003-01-28 Exelixis, Inc. Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use
WO2000056900A2 (en) 1999-03-22 2000-09-28 Novo Nordisk Biotech, Inc. Promoter sequences derived from fusarium venenatum and uses thereof
US6489127B1 (en) 2000-01-14 2002-12-03 Exelixis, Inc. Methods for identifying anti-cancer drug targets
US6515109B1 (en) 2000-10-12 2003-02-04 Exelixis, Inc. Human ECT2 polypeptide
WO2004090155A2 (en) * 2003-03-31 2004-10-21 Novozymes Inc. Methods for producing biological substances in enzyme-deficient mutants of aspergillus niger
WO2010039889A2 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell
WO2012160093A1 (en) 2011-05-23 2012-11-29 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
WO2017182442A1 (en) * 2016-04-19 2017-10-26 Novozymes A/S Rlma-inactivated filamentous fungal host cell

Non-Patent Citations (20)

* Cited by examiner, † Cited by third party
Title
CHRISTENSEN, BIOLTECHNOLOGY, vol. 6, 1988, pages 1419 - 1422
CULLEN ET AL., NUCLEIC ACIDS RES., vol. 15, 1987, pages 9163 - 9175
GEMS ET AL., GENE, vol. 98, 1991, pages 61 - 67
HAWKSWORTH ET AL.: "Ainsworth and Bisby's Dictionary of The Fungi", 1995, CAB INTERNATIONAL, UNIVERSITY PRESS
KENT C ET AL: "Interactions among pathways for phosphatidylcholine metabolism, CTP synthesis and secretion through the Golgi apparatus", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, AMSTERDAM, NL, vol. 24, no. 4, 1 April 1999 (1999-04-01), pages 146 - 150, XP027259161, ISSN: 0968-0004, [retrieved on 19990401] *
MALARDIER ET AL., GENE, vol. 78, 1989, pages 147 - 156
MAY, G.: "Applied Molecular Genetics of Filamentous Fungi", 1992, BLACKIE ACADEMIC AND PROFESSIONAL, pages: 1 - 25
MIKKO ARVAS ET AL: "Correlation of gene expression and protein production rate - a system wide study", BMC GENOMICS, BIOMED CENTRAL, vol. 12, no. 1, 20 December 2011 (2011-12-20), pages 616, XP021093245, ISSN: 1471-2164, DOI: 10.1186/1471-2164-12-616 *
MIKKO ARVAS ET AL: "Supplementary data to 'Correlation of gene expression and protein production rate -a system wide study' Supplementary Figures", BMC GENOMICS, 1 January 2011 (2011-01-01), XP055715727, Retrieved from the Internet <URL:https://static-content.springer.com/esm/art%3A10.1186%2F1471-2164-12-616/MediaObjects/12864_2011_3822_MOESM1_ESM.PDF> [retrieved on 20200716] *
MILLER ET AL., MOL. CELL. BIOL., vol. 5, 1985, pages 1714 - 1721
NEEDLEMANWUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
RICE ET AL., EMBOSS: THE EUROPEAN MOLECULAR BIOLOGY OPEN SOFTWARE SUITE, 2000
RICE ET AL.: "EMBOSS: The European Molecular Biology Open Software Suite", TRENDS GENET., vol. 16, 2000, pages 276 - 277, XP004200114, DOI: 10.1016/S0168-9525(00)02024-2
SAMBROOK,J.FRITSCH,E.F.MANIATIS,T.: "Molecular cloning: a laboratory manual", 1989, COLD SPRING HARBOR LABORATORY
TE BIESEBEKE R ET AL: "Branching mutants of Aspergillus oryzae with improved amylase and protease production on solid substrates", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 69, no. 1, 1 November 2005 (2005-11-01), pages 44 - 50, XP019331991, ISSN: 1432-0614, DOI: 10.1007/S00253-005-1968-4 *
TURNER, G.: "Vectors for Genetic Manipulation", 1994, ELSEVIER, pages: 641 - 665
VERA MEYER ET AL: "Reconstruction of Signaling Networks Regulating Fungal Morphogenesis by Transcriptomics", EUKARYOTIC CELL NOV 2015, vol. 8, no. 11, 11 September 2009 (2009-09-11), pages 1677 - 1691, XP055712994, ISSN: 1535-9786, DOI: 10.1128/ec.00050-09 *
YELTON ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 1470 - 1474
YELTON ET AL.: "Transformation of Aspergillus nidulans by using a trpC plasmid", PROC NATL ACAD SCI USA, vol. 81, no. 5, March 1984 (1984-03-01), pages 1470 - 4, XP000674725, DOI: 10.1073/pnas.81.5.1470
ZHIGANG XIE ET AL: "Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects Edited by", PROC NATL ACAD SCI, vol. 95, no. 25, 1 October 1998 (1998-10-01), pages 12346 - 12351, XP055715710 *

Also Published As

Publication number Publication date
US20220251581A1 (en) 2022-08-11
CN114555777A (zh) 2022-05-27
EP4004187A1 (en) 2022-06-01

Similar Documents

Publication Publication Date Title
CN104395454B (zh) 改善的真菌选择
WO2018015444A1 (en) Crispr-cas9 genome editing with multiple guide rnas in filamentous fungi
US20170313997A1 (en) Filamentous Fungal Double-Mutant Host Cells
US20190225988A1 (en) Genomic integration of DNA fragments in fungal host cells
EP3077514A1 (en) Fungal gene library by double split-marker integration
US11046736B2 (en) Filamentous fungal host
US20220025423A1 (en) Modified Filamentous Fungal Host Cells
WO2018172155A1 (en) Improved filamentous fungal host cells
EP3445776B1 (en) Rlma-inactivated filamentous fungal host cell
DK2652138T3 (en) Promoters for expression of genes in a fungal cell
JP2019122386A (ja) 選択的オートファジー経路の不活性化成分を含む糸状真菌細胞及びその使用方法
WO2018167153A1 (en) Improved filamentous fungal host cell
WO2021018783A1 (en) Modified filamentous fungal host cell
US11434475B2 (en) Modified filamentous fungal host cell for encoding a secreted polypetide of interest
WO2020112911A1 (en) Improved filamentous fungal host cells
US20230407273A1 (en) Glycosyltransferase variants for improved protein production
EP4237430A1 (en) Leader peptides and polynucleotides encoding the same
EP2585591A1 (en) Polynucleotides having leader sequence function

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20743153

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2020743153

Country of ref document: EP

Effective date: 20220228