WO2020186400A1 - 一种生长促进剂及其在防治十字花科作物根肿病中的应用 - Google Patents

一种生长促进剂及其在防治十字花科作物根肿病中的应用 Download PDF

Info

Publication number
WO2020186400A1
WO2020186400A1 PCT/CN2019/078345 CN2019078345W WO2020186400A1 WO 2020186400 A1 WO2020186400 A1 WO 2020186400A1 CN 2019078345 W CN2019078345 W CN 2019078345W WO 2020186400 A1 WO2020186400 A1 WO 2020186400A1
Authority
WO
WIPO (PCT)
Prior art keywords
growth promoter
parts
root
plant
growth
Prior art date
Application number
PCT/CN2019/078345
Other languages
English (en)
French (fr)
Inventor
刘勇
张蕾
黄小琴
伍文宪
杨潇湘
周西全
刘红雨
薛龙海
向运佳
刘俊豆
Original Assignee
四川省农业科学院植物保护研究所
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 四川省农业科学院植物保护研究所 filed Critical 四川省农业科学院植物保护研究所
Priority to PCT/CN2019/078345 priority Critical patent/WO2020186400A1/zh
Priority to CN201980003668.2A priority patent/CN111032598A/zh
Priority to CA3081636A priority patent/CA3081636C/en
Publication of WO2020186400A1 publication Critical patent/WO2020186400A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C05D9/02—Other inorganic fertilisers containing trace elements
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/14—Boron; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Definitions

  • the invention relates to a crop growth regulator, in particular to a growth promoter for restoring the absorption function of roots and its application in the prevention and treatment of root swelling of cruciferous crops.
  • Root swelling commonly known as "plant cancer” is an obligate parasitic soil-borne disease caused by the infection of Brassica spp. It mainly infects rape, cabbage, cabbage, cauliflower, kohlrabi, mustard, radish, turnip, etc. Flower plant. The disease mainly damages plant root tissues, causes root parenchyma cells to proliferate and forms tumors, makes it difficult for plants to absorb nutrients, and causes plants to wither, grow slowly, and even die; when conditions are suitable, it can cause yield and quality of cruciferous crops such as rapeseed. Decline, or even fail. According to incomplete statistics, the disease causes economic losses of cruciferous crops of more than 10% worldwide, and losses of more than 70% on certain crops.
  • the root swelling disease is a worldwide soil-borne disease.
  • the pathogen exists in the soil in the form of dormant spores. It can adapt to extremely harsh environments and survive for up to 15 years in the absence of susceptible host plants, making its prevention and control quite difficult.
  • fields with root swelling disease are generally no longer suitable for cultivation of cruciferous plants; in addition, traditional agricultural operations, such as early sowing of rape and long-term planting of cruciferous crops in the same field, will aggravate root swelling in diseased fields. Disease hazards. At present, due to the lack of effective varieties of disease-resistant spectroscopic root swelling, only chemical fungicides can be used in production.
  • the inventor of the present application created a seed coating agent that can be used to prevent root swelling disease, which has a control effect of more than 80% against root swelling disease in rape seedlings, which significantly reduces the amount of pesticides used and improves chemical control
  • the environmental compatibility of technology overcomes the technical barrier that prevents root swelling.
  • the seed coating agent is limited by the dosage of the drug and the duration of the drug effect, it is difficult to achieve effective prevention and control of root swelling in the later stage of rapeseed growth, and it cannot cure the diseased plants.
  • the yield of crops has a stable therapeutic effect on root swelling, is not easy to cause environmental pollution, and its effect in the field is not affected by geological conditions and soil environment. It can not only reconstruct or restore the normal roots of diseased plants Absorption function, promote crop growth and increase yield, significantly reduce the amount of pesticides used, improve the stability of the farmland ecosystem, reduce the planting cost of cruciferous crops, and overcome the technical bottleneck of root swelling diseased plants. Solving the major practical production problems of root swelling, which is "difficult to treat", will greatly stimulate the enthusiasm of planters and promote the sustainable and safe production of cruciferous crops.
  • the present invention provides a method for restoring the root absorption function of roots and can be used to prevent root swelling of cruciferous crops. Growth promoters.
  • the components of the growth promoter include in parts by weight: 0.3-0.5 parts of indole butyric acid, 0.3-0.5 parts of naphthaleneacetic acid, 15-20 parts of humic acid, 10-20 parts of boron (calculated as element B) and plant nutrition 30-50 parts.
  • the technical purpose of the present invention is achieved by using growth regulators (indole butyric acid and naphthalene acetic acid), humic acid, boron and plant nutrients to cooperate with each other, and compounding according to appropriate components and ratios.
  • both indole butyric acid and naphthalene acetic acid are compounds that can be used in agriculture.
  • Humic acid is, for example, sodium humate/or potassium humate.
  • Boron is at least one of boric acid, borax, and boron-magnesium fertilizer.
  • the infection process of rhizobia is divided into two stages: root hair infection stage and cortex infection stage.
  • the root hair infection stage is when dormant spores germinate and infect the root hair and epidermal cells of the plant. The infection at this stage does not cause obvious symptoms of the host plant, nor does it directly cause the yield or quality of the host plant to decrease.
  • the cortical infection stage is when the primary zoosporangia releases the secondary zoospores to infect the cortical cells, leading to the proliferation and hypertrophy of the root tissue cells and the formation of swollen roots. This stage is the stage where pathogens proliferate in large numbers in the host plant, and the roots are severely enlarged.
  • Indole butyric acid an endogenous growth regulator, can promote cell division and cell growth, induce adventitious root formation, increase fruit setting, prevent fruit dropping, and change the ratio of female to male flowers. It can enter the plant body through the tender cuticles of leaves, branches and seeds, and be transported to the functional parts along with the nutrient flow. At present, it is mainly used for rooting cuttings, which can induce the formation of root protozoa, promote cell differentiation and division, facilitate the formation of new roots and the differentiation of the vascular bundle system, and promote the formation of adventitious roots of cuttings. Indolebutyric acid is generally used for transplanting woody and herbaceous plants by soaking roots, or soaking and dressing seeds.
  • Naphthalene acetic acid is a broad-spectrum plant growth regulator that can promote cell division and expansion, induce the formation of adventitious roots, increase fruit setting, prevent fruit dropping, and change the ratio of female to male flowers.
  • the seeds can enter the plant through the tender skin of leaves and branches, and be transported to the whole plant along with the nutrient flow.
  • Previous studies have shown that in the process of plant growth, the use of a certain amount of naphthalene acetic acid or sodium naphthalene acetate can significantly promote plant growth and increase yield.
  • the papaya seeds were treated with different concentrations of naphthalene acetic acid. The results showed that NAA improved the overall germination rate, germination potential and vigor index of seeds, and could promote the photosynthesis of different plants.
  • Humic acid is a macromolecular organic substance that exists widely in nature. It is widely used in various industries such as agriculture, forestry, animal husbandry, and chemical industry. It is mainly used in agricultural production to produce fertilizers (such as ammonium humate, potassium humate, etc.) ), the application of humic acid in the soil can significantly improve the physical and chemical properties of the soil and enhance the fertilizer efficiency. Some studies have shown that the application of humic acid in the soil can increase the content of microorganisms in the soil, increase the utilization of nutrients by crops, and significantly increase the biomass of crops such as sweet potatoes, grapes, cabbage and tomatoes. Appropriate addition of humic acid can improve the root vigor of normal-growing rape seedlings, significantly promote root growth, and increase the antioxidant enzyme activity and chlorophyll content in rape cells.
  • Boron is a trace element that provides plant nutrients for its main functions.
  • Conventional boron refers to boron chemical products mainly based on borax, boric acid, boron magnesium fertilizer, etc., as a trace element fertilizer for agriculture.
  • Boron is one of the essential nutrient elements for plants. It is absorbed and utilized by plants in the form of boric acid molecules (H 3 BO 3 ), and is not easy to move in plants. Boron can promote root growth and play an important role in the synthesis and transport of photosynthesis products-carbohydrates. The main role is to have a special effect on the normal process of fertilization. Rape is very sensitive to boron. Boron deficiency will cause "flowers but not real", resulting in a large-scale reduction in production.
  • the plant nutrient supplements the various elements of plant growth to promote the healthy growth of plants.
  • the main components of the plant nutrient in the present invention are nitrogen, phosphorus and potassium. These three substances are required by the plant and taken away during harvest. There are many nutrients, but they are not returned to the soil in the form of residues and roots. Therefore, it is often necessary to supplement these nutrients by applying fertilizers. Rape is a crop that requires a lot of fertilizer and has strong fertility tolerance. Nitrogen, phosphorus and potassium are essential elements for the growth of rape.
  • the inventors have discovered in a long-term study that even if the damaged root system infested by rhizobium fungus is completely decayed, it will not spread to the adjacent healthy root tissues. Only a few healthy roots can continue to maintain the nutrient and water supply of the above-ground plants. , And make the diseased plants in a growing state; combined with the theory of plant cell pluripotency, it is speculated that the diseased plants can be induced to grow new roots through external stimuli, and the normal physiological functions of the roots of the injured plants can be restored through reconstruction.
  • the growth promoter of the present invention is to combine indole butyric acid, naphthalene acetic acid, humic acid, boron and plant growth agent according to suitable components and ratios, and select the special sensitive period of the plant according to the principle of mutual cooperation of each component It can re-establish the water and nutrient absorption function of the root system of diseased plants with root swelling, and restore the normal growth of diseased plants that are dying.
  • the dosage of indolebutyric acid is 0.3-0.5g/mu, and the water consumption is 40-50L/667m 2 .
  • the corresponding ratio, the dosage of naphthalene acetic acid is 0.3-0.5g/667m 2 ; the dosage of humic acid is 15-20g/667m 2 ; the dosage of boron is 10-20g/667m 2 ; the dosage of plant nutrients is 30-50g /667m 2 ;
  • the dosage of this kind of medicine is greatly reduced, and the cost of medicine is also greatly reduced.
  • the present invention provides a growth promoter that restores root absorption and can be used to prevent root swelling of cruciferous crops. On the one hand, it can meet the nutritional needs of rape and other cruciferous crops, and on the other hand, it can be induced by exogenous stimulation.
  • the root system grows, re-establishes the normal physiological function of the root system of the diseased plant and restores the "vitality" of the diseased plant on the verge of death. It is suitable for the prevention and treatment of root swelling of cruciferous crops such as rape and cabbage.
  • the present invention has the advantages of: firstly, less dosage, significant effect, high benefit, more application objects and wide application range; secondly, it can re-establish the water and nutrients of the root system of diseased roots
  • the special way of absorption function restores the normal growth of diseased plants that are on the verge of death, so that the survival rate of diseased plants can reach more than 90%, and the output can be increased by more than 60%, ensuring the output of rape and cruciferous crops, and curing root swelling
  • the effect is outstanding and stable; thirdly, it overcomes the technical bottleneck of the diseased plants of root swelling that is difficult to live and cure. It does not contain chemical pesticides, is green and environmentally friendly, and is convenient and quick to use.
  • a growth promoter calculated according to the amount of effective ingredients per 667m 2 of rape, consists of the following components:
  • a growth promoter calculated according to the amount of effective ingredients per mu of rape, consists of the following components:
  • a growth promoter calculated according to the amount of effective ingredients per 667m 2 of rape, consists of the following components:
  • a growth promoter calculated according to the amount of effective ingredients per 667m 2 of rape, consists of the following components:
  • a growth promoter calculated according to the amount of effective ingredients per 667m 2 of rape, consists of the following components:
  • a growth promoter containing only 0.4g naphthaleneacetic acid is a growth promoter containing only 0.4g naphthaleneacetic acid.
  • a growth promoter that only contains 0.4g of boron is a growth promoter that only contains 0.4g of boron.
  • This comparative example does not add any medicament, only sprays with clean water.
  • the treatment method is as follows: randomly select 3 test plots and spray the water/solution of Example 1-5 and Comparative Example 1-5 respectively, that is, spray the agent of Example 1 on 3 plots, and so on; the test results are averaged value;

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Inorganic Chemistry (AREA)
  • Forests & Forestry (AREA)
  • Ecology (AREA)
  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

本发明公开了一种生长促进剂及其在防治十字花科作物根肿病中的应用，包括下述重量份的组分：吲哚丁酸0.3-0.5份、萘乙酸0.3-0.5份、腐殖酸15-20份、硼10-20份、植物营养剂30-50份；本发明的恢复根部吸收功能的生长促进剂，采用合适的组分和比例，相互协同，采用简单的叶面喷施方式，一方面可以重新建立根肿病患病植株根系的水分和养分吸收功能、恢复濒临死亡的患病植株的正常生长，使患病植株的成活率达到90％以上，产量增加60％以上；另一方面，该促进剂的使用方便快捷，攻克了根肿病患病难活的技术瓶颈，可以有效降低劳动力成本80％以上，同时也降低了使用化学农药带来的食品安全和环境污染的潜在风险，社会、经济和生态效益显著。

Description

一种生长促进剂及其在防治十字花科作物根肿病中的应用 技术领域
本发明涉及作物生长调节剂，尤其涉及一种恢复根部吸收功能的生长促进剂及其在防治十字花科作物根肿病中的应用。
背景技术
根肿病俗称“植物癌症”，是由芸苔根肿菌侵染引起的专性寄生性土传病害，主要侵染油菜、甘蓝、白菜、花椰菜、撇蓝、芥菜、萝卜、芜菁等十字花科植物。该病主要为害植物根部组织、引起根部薄壁细胞增生而形成肿瘤，使植株养分吸收困难，导致植物萎焉、生长缓慢，甚至死亡；在条件适合时可以导致油菜等十字花科作物产量和品质下降，甚至绝收。据不完全统计，该病在世界范围内导致十字花科作物经济损失达10％以上，在某些作物上的损失达70％以上，在中国，根肿病已覆盖油菜和十字花科蔬菜主栽区，据不完全统计，目前中国油菜根肿病发病面积至少在60万公顷以上，可致每公顷油菜减产1500公斤以上，严重威胁着中国油菜及十字花科蔬菜产业的发展。
根肿病为世界性土传性病害，该病原菌以休眠孢子形式存在于土壤中，在缺少感病寄主植物时可以适应极其恶劣的环境并生存长达15年之久，导致其防治相当困难，且发生根肿病的田块，一般不再适宜栽培十字花科植物；此外，传统农事操作，如油菜的早播和长期在同一田块种植十字花科作物，都会加重发病田块的根肿病危害。目前，由于缺乏有效的光谱性根肿病抗病品种，生产 上只能采用化学杀菌剂进行防治，不仅药剂用量大，药剂和劳动力成本高，加之在表现症状后再进行药剂处理，不但没有防治效果，还会因大量药剂土壤处理带来环境污染和人体健康的潜在风险。因此，根肿病“难防难治”的突出问题，不仅严重打击了农民种植十字花科作物的积极性，而且对油菜及十字花科蔬菜产业的可持续发展构成了极大威胁，迫切需要探索新的根肿病防治策略。
本申请发明人在前期工作中，创制了可用于防治根肿病的种子包衣剂，对油菜苗期根肿病的防效可达80％以上，显著降低了农药使用量，提升了化学防治技术的环境亲和性，攻克了根肿病难防的技术屏障。但是，由于种子包衣剂受药剂剂量和药效持效期的限制，难以实现对油菜生长后期根肿病的有效防控，且不能治愈已经患病的植株。如能提供一种不含化学农药、绿色环保、并能够重新构建或恢复患根肿病植株根系的正常吸收养分和水分的功能，恢复濒临死亡患病植株的正常生长、保障油菜及十字花科作物的产量，对根肿病治疗效果稳定，不易造成环境污染，且在田间的使用效果不受地质条件、土壤环境影响的新型植物生长促进剂，不仅能重新构建或恢复患病植株根部正常的吸收功能、促进作物生长和提高产量，显著降低农药使用量，提升农田生态系统稳定性，降低十字花科作物的种植成本，还能攻克根肿病患病植株患病难活的技术瓶颈，真正解决根肿病“难治”的重大生产实际问题，将大大激励广大种植户的种植积极性、助推十字花科作物的可持续安全生产。
发明内容
为了克服现有防治技术的缺点，攻克根肿病患病植株“患病难活”的技术瓶颈，本发明提供了一种恢复根部的根系吸收功能并可用于防治十字花科作物 根肿病的生长促进剂。
该生长促进剂的组分按重量份包括：吲哚丁酸0.3-0.5份、萘乙酸0.3-0.5份、腐殖酸15-20份、硼10-20份(以B元素计)和植物营养剂30-50份。本发明的技术目的是通过采用生长调节剂(吲哚丁酸和萘乙酸)、腐殖酸、硼和植物营养剂相互协同作用、按照合适的组分和比例进行复配来实现的。
本发明中，吲哚丁酸、萘乙酸均采用农业可用的化合物。腐殖酸为如腐植酸钠/或腐植酸钾。硼为硼酸、硼砂、硼镁肥中至少一种。植物营养剂为氮、磷、钾的复合剂，其质量比优选为N：P：K＝4：1：1。
前人研究已经证实，根肿菌的侵染过程分为两个阶段：根毛侵染阶段和皮层侵染阶段。根毛侵染阶段是休眠孢子萌发侵染植株根毛和表皮细胞，该阶段侵染并不会引起寄主植物出现明显的病症，也不直接造成寄主植物产量或质量下降。皮层侵染阶段是初级游动孢子囊释放次级游动孢子侵染皮层细胞，导致根部组织细胞增殖、肥大并形成肿根，此阶段是病原菌在寄主植物内大量增殖的阶段、根部肿大严重影响植株对水分、养分的吸收，直至萎蔫、死亡。根肿病在表现症状后再进行药剂防治，只能杀死部分残留在土壤中的病原菌，而对寄主植物体内的病原菌没有效果、更加无法恢复根部的正常吸收水分和养分功能，故根肿病患病植株“患病难活”。
吲哚丁酸，是内源生长调节物质，能促进细胞分裂与细胞生长，诱导形成不定根，增加座果，防止落果，改变雌、雄花比率等。可经由叶片、树枝的嫩表皮、种子进入到植物体内，随营养流输导到起作用的部位。目前主要用于用于插条生根，可诱导根原体的形成、促进细胞分化和分裂、有利于新根生成和维管束系统的分化和促进插条不定根的形成。吲哚丁酸的使用方式一般为木 本和草本植物采用浸根移栽，也可以浸种、拌种。
萘乙酸(NAA)，是一种广谱型植物生长调节剂，能促进细胞分裂与扩大，诱导形成不定根增加座果，防止落果，改变雌、雄花比率等。可经叶片、树枝的嫩表皮，种子进入到植株内，随营养流输导到全株。前人研究表明，在植物生长过程中，使用一定量的萘乙酸或萘乙酸钠，可以显著促进植物生长、提高产量。采用不同浓度的萘乙酸处理番木瓜种子，结果表明，NAA提高了种子整体发芽率、发芽势和活力指数，可以促进不同植物的光合作用。
腐殖酸，是自然界中广泛存在的大分子有机物质，广泛用在农、林、牧、化工等各产业，在农业生产中主要用于生产肥料(如腐殖酸铵、腐殖酸钾等)，在土壤中施用腐殖酸可以明显改善土壤的理化性状，增强肥效。一些研究表明土壤施用腐殖酸后能增加土壤中微生物含量，提高作物对营养物质的利用率，显著增加甘薯、葡萄、白菜和番茄等作物的生物量。适量添加腐殖酸可提高正常生长油菜幼苗的根系活力、显著促进根系生长、提高油菜细胞内抗氧化酶活性和叶绿素含量。
硼，是以提供植物养分为其主要功效的微量元素，常规硼是指以硼砂、硼酸、硼镁肥等为主的硼化工制品作为农业用的微量元素肥料。硼是植物必需的营养元素之一，以硼酸分子(H 3BO 3)的形态被植物吸收利用，在植物体内不易移动。硼能促进根系生长，对光合作用产物-碳水化合物的合成与转运有重要作用，主要作用是对受精过程的正常进行有特殊作用。油菜对硼十分敏感，缺硼会出现“花而不实”，造成大面积减产。研究表明，施硼能够显著增加油菜单株角果数、单角果粒数、千粒重和产量。但是硼施用不当易造成作物硼中毒。也有学者发现施用一定比例的钙肥和硼肥可以控制根肿病的发生。
植物营养剂，是补充植物生长的各种元素、以促进植物健康生长，本发明中的植物营养剂主要成分为氮、磷、钾，这三种物质是植物需要量和收获时带走量较多的营养元素，而它们通过残体和根的形式归还给土壤的数量却不多，因此往往需要以施用肥料的方式补充这些养分。油菜是一种需肥量多、耐肥性强的作物，氮磷钾是油菜生长所需的必须元素。研究表明，油菜氮、磷、钾和硼肥如果单一施用某一种、两种或是三种肥料都难以显著提升菜籽产量，而氮磷钾和硼的合理配施可明显促进油菜生长，提高菜籽产量。
本发明人在长期研究中发现，被根肿菌侵染的受害根系即使完全腐烂也不向相邻的健康根系组织扩散危害，仅存的少数健康根系能继续维持地上部植株的养分和水分供给，并使患病植株处于生长状态；结合植物细胞全能性理论，推测可以通过外界刺激方式诱发患病植株长出新的根系、通过重新构建并恢复受害植株根系的正常生理功能。通过连续多年的研究结果表明，突破了根系生长基本上依靠土壤施用的传统模式，创造性地通过叶面喷雾方式和本发明的生长促进剂配方，完全实现了恢复患病植株的健康生长、重建了患病植株根系的生理功能，这种通过对根肿病受害植株进行叶面处理的特殊治疗方式，恢复了受害植株的“生命力”。该技术田间应用时可显著减少病害损失，使患病植株的成活率达到90％以上，产量增加60％以上。
本发明的生长促进剂是将吲哚丁酸、萘乙酸、腐殖酸、硼和植物生长剂按照合适的组分和比例组合、根据各组分相互协同作用的原理，选择植株的特殊敏感时期，可以重新建立根肿病患病植株根系的水分和养分吸收功能、恢复濒临死亡的患病植株的正常生长，在油菜抽薹初期、田间土温高于气温时/或十字花科作物根肿病患病植株还有1/5正常根系时，采用叶面喷雾的方式施用； 相对于传统的从根部用药的方式(需要在特定的时间内，前后不能超过3天)，使用的时间范围更宽泛，更容易操作，实用性更强。
优选吲哚丁酸的用量为0.3-0.5g/亩，用水量为40-50L/667m 2。相应的比例，萘乙酸的用量为0.3-0.5g/667m 2；腐殖酸的用量为15-20g/667m 2；硼的用量为10-20g/667m 2；植物营养剂的用量为30-50g/667m 2；这样的用量，相比现有技术通过根部施用，药剂用量大幅减少，药剂成本也就大幅减少。
本发明提供一种恢复根部吸收功能并可用于防治十字花科作物根肿病的生长促进剂，一方面可满足了油菜等十字花科作物生长的营养需求，另一方面通过外源刺激方式诱发根系生长、重新建立患病植株根系正常生理功能并恢复濒临死亡患病植株的“生命力”。适用于油菜、白菜等十字花科作物根肿病的防治和治疗。
与现有技术相比，本发明的优点在于：首先，用量少，效果显著，效益高，施用对象多，应用范围广；其次，可以通过重新建立根肿病患病植株根系的水分和养分吸收功能的特殊方式，恢复濒临死亡的患病植株的正常生长，使患病植株的成活率达到90％以上，产量增加60％以上，保障油菜及十字花科作物的产量，对根肿病治疗效果突出稳定；第三，攻克了根肿病患病植株难活难治的技术瓶颈，不含化学农药、绿色环保，使用方便快捷，相对于传统的根部防治方法，药剂成本和劳动力成本降低80％以上；第四，叶面施用的效果不受地质条件、土壤环境影响，显著降低了大量施用化学农药带来的食品安全和环境污染的潜在风险，社会、经济和生态效益显著。
具体实施方式
下面将结合实施例对本发明作进一步说明。
实施例1：
一种生长促进剂，按每667m 2油菜的有效成分用量计算，由下述组分组成：
Figure PCTCN2019078345-appb-000001
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
实施例2：
一种生长促进剂，按每亩油菜的有效成分用量计算，由下述组分组成：
Figure PCTCN2019078345-appb-000002
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
实施例3：
一种生长促进剂，按每667m 2油菜的有效成分用量计算，由下述组分组 成：
Figure PCTCN2019078345-appb-000003
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
实施例4：
一种生长促进剂，按每667m 2油菜的有效成分用量计算，由下述组分组成：
Figure PCTCN2019078345-appb-000004
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
实施例5：
一种生长促进剂，按每667m 2油菜的有效成分用量计算，由下述组分组成：
Figure PCTCN2019078345-appb-000005
Figure PCTCN2019078345-appb-000006
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
对比实施例1
一种生长促进剂，仅含有腐殖酸 16g；
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
对比实施例2
一种生长促进剂，仅含有吲哚丁酸 0.4g。
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
对比实施例3
一种生长促进剂，仅含有萘乙酸 0.4g。
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
对比例4
一种生长促进剂，仅含有硼元素 0.4g。
施用时，将上述生长促进剂兑水50kg全部溶解后，喷施667m 2。
对比例5
本对比例不加入任何药剂，仅用清水喷施。
实验验证
于2017年11月在广汉西高镇，选取30块油菜(13片真叶期)根肿病高发田块(每块田面积为667m 2)进行平行试验，试验田选取标准为：80％以上油菜感染根肿病；
处理方法为：随机选取3块试验田分别叶面喷施实施例1-5和对比例1-5的水/溶液，即实施例1的药剂喷施3块田，以此类推；试验结果取平均值；
试验结果见表1
表1试验结果表
Figure PCTCN2019078345-appb-000007
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本发明的保护范围之内。

Claims (10)

  1. 一种生长促进剂，其特征在于，按重量份计，各组分包括：吲哚丁酸0.3-0.5份、萘乙酸0.3-0.5份、腐殖酸15-20份、硼10-20份和植物营养剂30-50份。
  2. 根据权利要求1所述的生长促进剂，其特征在于，所述腐殖酸为腐植酸钠和/或腐植酸钾。
  3. 根据权利要求1所述的生长促进剂，其特征在于，所述硼为硼酸、硼砂、硼镁肥中至少一种。
  4. 根据权利要求1所述的生长促进剂，其特征在于，所述植物营养剂为氮、磷、钾的复合剂。
  5. 根据权利要求4所述的生长促进剂，其特征在于，所述氮、磷、钾的质量比为N：P：K＝4：1：1。
  6. 权利要求1所述的生长促进剂在防治十字花科作物根肿病中的应用。
  7. 根据权利要求6所述的应用，其特征在于，采用叶面喷雾的方式施用。
  8. 根据权利要求7所述的应用，其特征在于，所述叶面喷施的时间为：在油菜抽薹初期、田间土温高于气温时/或十字花科作物根肿病患病植株还有1/5正常根系时。
  9. 根据权利要求7所述的应用，其特征在于，所述吲哚乙酸的施用量为0.3-0.5g/667m 2，用水量为40-50L/667m 2。
  10. 根据权利要求6所述的应用，其特征在于：所述十字花科作物至少包括油菜、抱子芥或大白菜。
PCT/CN2019/078345 2019-03-15 2019-03-15 一种生长促进剂及其在防治十字花科作物根肿病中的应用 WO2020186400A1 (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PCT/CN2019/078345 WO2020186400A1 (zh) 2019-03-15 2019-03-15 一种生长促进剂及其在防治十字花科作物根肿病中的应用
CN201980003668.2A CN111032598A (zh) 2019-03-15 2019-03-15 一种生长促进剂及其在防治十字花科作物根肿病中的应用
CA3081636A CA3081636C (en) 2019-03-15 2019-03-15 Growth promoter and application thereof for prevention and treatment of clubroot on cruciferous crops

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/078345 WO2020186400A1 (zh) 2019-03-15 2019-03-15 一种生长促进剂及其在防治十字花科作物根肿病中的应用

Publications (1)

Publication Number Publication Date
WO2020186400A1 true WO2020186400A1 (zh) 2020-09-24

Family

ID=70198291

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2019/078345 WO2020186400A1 (zh) 2019-03-15 2019-03-15 一种生长促进剂及其在防治十字花科作物根肿病中的应用

Country Status (3)

Country Link
CN (1) CN111032598A (zh)
CA (1) CA3081636C (zh)
WO (1) WO2020186400A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112449957A (zh) * 2020-12-01 2021-03-09 四川省农业科学院植物保护研究所 一种提高油菜毯状育苗植株移栽成活率的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103694045A (zh) * 2013-12-18 2014-04-02 徐跃华 一种用于农作物增效补充营养和抗病增产的复合制剂及其制备方法和应用
CN104003811A (zh) * 2014-06-16 2014-08-27 四川省农业科学院植物保护研究所 防治十字花科作物根肿病的生根剂、制备方法和用途
CN107686405A (zh) * 2017-09-20 2018-02-13 荆门市东栗农特技术服务中心 一种防治油菜根肿病液肥
CN108530186A (zh) * 2018-06-15 2018-09-14 山东罗纳朗克生物农化有限公司 一种用于防治土传根部病害具有刺激作物生长作用的药肥
CN108929152A (zh) * 2018-07-12 2018-12-04 广州恒盛园林股份有限公司 一种园林树木复壮营养液及其制备方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103694045A (zh) * 2013-12-18 2014-04-02 徐跃华 一种用于农作物增效补充营养和抗病增产的复合制剂及其制备方法和应用
CN104003811A (zh) * 2014-06-16 2014-08-27 四川省农业科学院植物保护研究所 防治十字花科作物根肿病的生根剂、制备方法和用途
CN107686405A (zh) * 2017-09-20 2018-02-13 荆门市东栗农特技术服务中心 一种防治油菜根肿病液肥
CN108530186A (zh) * 2018-06-15 2018-09-14 山东罗纳朗克生物农化有限公司 一种用于防治土传根部病害具有刺激作物生长作用的药肥
CN108929152A (zh) * 2018-07-12 2018-12-04 广州恒盛园林股份有限公司 一种园林树木复壮营养液及其制备方法

Also Published As

Publication number Publication date
CA3081636A1 (en) 2020-09-15
CN111032598A (zh) 2020-04-17
CA3081636C (en) 2023-08-01

Similar Documents

Publication Publication Date Title
Bindhu Effect of Azolla extract on growth performance of Pisum sativum
Singh et al. Effect of plant growth regulators on growth and yield of coriander
Kiran et al. Seed yield and quality of brinjal as influenced by crop nutrition
Soad et al. Influence of foliar application of pepton on growth, flowering and chemical composition of Helichrysum bracteatum plants under different irrigation intervals
El-Dayem Effect of silicon and algae extract foliar application on growth and early yield of globe artichoke plants.
Prisa et al. Microbic and Algae biofertilizers in Aloe barbadensis Miller
CN102344323B (zh) 一种棉花专用液体增花保果防虫药肥及其制备方法
CN112679270A (zh) 一种适用于新疆滴灌棉花的调控药肥及其制备方法
WO2020186400A1 (zh) 一种生长促进剂及其在防治十字花科作物根肿病中的应用
CN104045457A (zh) 灭菌生根剂及制备方法
Anwar et al. Phosphorus management improve productivity of wheat varieties under semiarid climates
Maleki et al. Physiological responses of sweet basil (Ocimum basilicum L.) to triple inoculation with Azotobacter, Azospirillum, Glomus intraradices and foliar application of citric acid
CN102432391A (zh) 液体型抗棉花枯黄萎病土壤调理剂及其生产方法和使用方法
Gupta et al. Integrated nutrient management for growth and yield in Glory Lily (Gloriosa superba L.)
CN110754362B (zh) 一种富硒金线莲的培养基及其培育方法
CN105777268B (zh) 一种适用于烟苗生长防治向日葵列当的复合有机肥
Rather et al. Influence of nitrogen, copper and zinc nanofertilizers on growth characteristics of chilli (Capsicum annuum var. annuum L.)
Nehete et al. Effect of bio-fertilizers in combination with chemical fertilizers on flowering, yield and quality of mango (Mangifera indica L.) cv. Amrapali
Sutariya et al. Effect of integrated nutrient management on bio chemical parameters of phalsa (Grewia subinaequalis L.) cv. local
Merwad Effect of nitrogen sources, rates, some biostimulants and antioxidants on growth and productivity of banana plants
Singh et al. Effect of foliar application of urea and NAA on yield and yield attributes of chilli (Capsicum annuum var: longum)
Patil et al. Response of Sancerre gladiolus to different levels of nitrogen and phosphorus application under south Gujarat condition
Sathish et al. Influence of bunch feeding and bunch spraying on Post-harvest quality of tissue culture banana cv. Ney poovan
Taha The complementary effect of biostimulants and nitrogen fertilizer on improving the growth and productivity of squash (Cucurbita pepo L.)
Thingujam et al. Integrated nutrient management in brinjal-A review study

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19920525

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19920525

Country of ref document: EP

Kind code of ref document: A1