WO2020141559A3 - Device and method for multidimensional cell culture - Google Patents

Device and method for multidimensional cell culture Download PDF

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Publication number
WO2020141559A3
WO2020141559A3 PCT/IN2020/050011 IN2020050011W WO2020141559A3 WO 2020141559 A3 WO2020141559 A3 WO 2020141559A3 IN 2020050011 W IN2020050011 W IN 2020050011W WO 2020141559 A3 WO2020141559 A3 WO 2020141559A3
Authority
WO
WIPO (PCT)
Prior art keywords
dimension
cell culture
present
tissueoids
ability
Prior art date
Application number
PCT/IN2020/050011
Other languages
French (fr)
Other versions
WO2020141559A2 (en
Inventor
Kundu PRABUDDHA KUMAR
Das Avijit
Arora NUPUR MEHROTRA
Mazumdar SAUMYABRATA
Baru AMBICA
Original Assignee
Premas Biotech Private Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Premas Biotech Private Limited filed Critical Premas Biotech Private Limited
Priority to KR1020217024605A priority Critical patent/KR20210124233A/en
Priority to EP20736006.6A priority patent/EP3906299A4/en
Priority to CA3125540A priority patent/CA3125540A1/en
Priority to JP2021538765A priority patent/JP2022516905A/en
Priority to US16/775,461 priority patent/US20200326330A1/en
Publication of WO2020141559A2 publication Critical patent/WO2020141559A2/en
Publication of WO2020141559A3 publication Critical patent/WO2020141559A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Abstract

The present invention discloses a device and method for multidimensional cell culture, a more particularly three-dimension (3D) and four-dimension (4D) device and method. The device and method of the present invention comprises growing cells as spheroids/tissueoids on non-woven fabric scaffold to create 3D tissue-like structures. The fourth dimension is provided by ability of the system to generate the 3D tissueoids in a much less time span and their ability to grow for extended period of time, even for greater than one year. The present invention also provides methods of use for analysis of cell-drug sensitivity of the device. Further, the invention provides a device for growth and drug sensitivity characterization of cells.
PCT/IN2020/050011 2019-01-04 2020-01-04 Device and method for multidimensional cell culture WO2020141559A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
KR1020217024605A KR20210124233A (en) 2019-01-04 2020-01-04 Multidimensional cell culture apparatus and method
EP20736006.6A EP3906299A4 (en) 2019-01-04 2020-01-04 Device and method for multidimensional cell culture
CA3125540A CA3125540A1 (en) 2019-01-04 2020-01-04 Device and method for multidimensional cell culture
JP2021538765A JP2022516905A (en) 2019-01-04 2020-01-04 Equipment and methods for multidimensional cell culture
US16/775,461 US20200326330A1 (en) 2019-01-04 2020-01-29 Device and method for multidimensional cell culture

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201911000546 2019-01-04
IN201911000546 2019-01-04

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US16/775,461 Continuation US20200326330A1 (en) 2019-01-04 2020-01-29 Device and method for multidimensional cell culture

Publications (2)

Publication Number Publication Date
WO2020141559A2 WO2020141559A2 (en) 2020-07-09
WO2020141559A3 true WO2020141559A3 (en) 2020-09-17

Family

ID=71407309

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2020/050011 WO2020141559A2 (en) 2019-01-04 2020-01-04 Device and method for multidimensional cell culture

Country Status (5)

Country Link
EP (1) EP3906299A4 (en)
JP (1) JP2022516905A (en)
KR (1) KR20210124233A (en)
CA (1) CA3125540A1 (en)
WO (1) WO2020141559A2 (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4833083A (en) * 1987-05-26 1989-05-23 Sepragen Corporation Packed bed bioreactor
US6218182B1 (en) * 1996-04-23 2001-04-17 Advanced Tissue Sciences Method for culturing three-dimensional tissue in diffusion gradient bioreactor and use thereof
US6372495B1 (en) * 1995-10-06 2002-04-16 Seed Capital Investments-2 (Sci-2) B.V. Bio-artificial organ containing a matrix having hollow fibers for supplying gaseous oxygen
US7682822B2 (en) * 2006-03-31 2010-03-23 Aastrom Biosciences, Inc. Ex vivo generated tissue system
US20110313540A1 (en) * 2008-12-17 2011-12-22 Clare Selden in or relating to growing cells
US8367410B2 (en) * 2003-06-20 2013-02-05 Massachusetts Institute Of Technology Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US8911995B2 (en) * 2007-08-22 2014-12-16 Probiogen Ag Culture system and method for immunogenicity and immunofunction testing in vitro
WO2017212309A1 (en) * 2016-06-06 2017-12-14 Pluristem Ltd. Altered adherent stromal cells and methods of producing and using same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5266476A (en) * 1985-06-18 1993-11-30 Yeda Research & Development Co., Ltd. Fibrous matrix for in vitro cell cultivation
GB0815427D0 (en) * 2008-08-22 2008-10-01 Capsant Neurotechnologies S A Device
JP6813356B2 (en) * 2013-08-02 2021-01-13 ユニバーシティ・オブ・サウス・フロリダ 3D fibrous scaffold for cell culture
EP3135752A4 (en) * 2014-04-22 2018-03-14 Nippon Shokubai Co., Ltd. Cell culture substrate containing fluorine-containing polymer on surface
US11384328B2 (en) * 2015-11-18 2022-07-12 President And Fellows Of Harvard College Cartridge-based system for long term culture of cell clusters

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4833083A (en) * 1987-05-26 1989-05-23 Sepragen Corporation Packed bed bioreactor
US6372495B1 (en) * 1995-10-06 2002-04-16 Seed Capital Investments-2 (Sci-2) B.V. Bio-artificial organ containing a matrix having hollow fibers for supplying gaseous oxygen
US6218182B1 (en) * 1996-04-23 2001-04-17 Advanced Tissue Sciences Method for culturing three-dimensional tissue in diffusion gradient bioreactor and use thereof
US8367410B2 (en) * 2003-06-20 2013-02-05 Massachusetts Institute Of Technology Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US7682822B2 (en) * 2006-03-31 2010-03-23 Aastrom Biosciences, Inc. Ex vivo generated tissue system
US8911995B2 (en) * 2007-08-22 2014-12-16 Probiogen Ag Culture system and method for immunogenicity and immunofunction testing in vitro
US20110313540A1 (en) * 2008-12-17 2011-12-22 Clare Selden in or relating to growing cells
WO2017212309A1 (en) * 2016-06-06 2017-12-14 Pluristem Ltd. Altered adherent stromal cells and methods of producing and using same

Also Published As

Publication number Publication date
JP2022516905A (en) 2022-03-03
KR20210124233A (en) 2021-10-14
CA3125540A1 (en) 2020-07-09
WO2020141559A2 (en) 2020-07-09
EP3906299A4 (en) 2022-10-19
EP3906299A2 (en) 2021-11-10

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