WO2020125357A1

WO2020125357A1 - Integrating-sphere detection device

Info

Publication number: WO2020125357A1
Application number: PCT/CN2019/121174
Authority: WO
Inventors: 董方
Original assignee: 东方伊诺(苏州)医疗科技有限公司
Priority date: 2018-12-20
Filing date: 2019-11-27
Publication date: 2020-06-25
Also published as: CN209513613U; JP3235429U

Abstract

An integrating-sphere detection device comprises a housing (1), an inner chamber (2) disposed in the housing (1), and a light source (3) and a light sensor (4) disposed at the housing (1). The interior of the inner chamber (2) is provided with a spherical detection cavity (20), an entrance port (21) allowing light emitted from the light source (3) to enter the detection cavity (20), an exit port (22) allowing the emitted light to hit a test film, and a detection port (23) allowing light diffusely reflected by the detection cavity to hit the light sensor. The integrating-sphere detection device further comprises a light shielding plate (5) preventing the emitted light from being directly reflected to the light sensor (4) by the test film (6). The light shielding plate (5) is disposed in the detection cavity and located between the exit port (22) and the detection port (23).

Description

Integrating sphere detection device

Technical field

The invention belongs to the technical field of analysis and detection, and relates to an integrating sphere detection device, in particular to an integrating sphere detection device of an immunodetection analyzer, and is particularly suitable for immunodetection using a porous substance as a carrier.

Background technique

The integrating sphere detection device is one of the important components of the analyzer, which is mostly used for solid sample sampling. Irradiate the sample through an integrating sphere and collect the light signal after diffuse reflection of the sample. As disclosed in Chinese patent CN205910112U, a spectrometer integrating sphere includes an integrating sphere, a light-transmitting spot row, a sample cup, and a sample cup rotation driving component. The length of the light-transmitting spot row covers exactly the radius length range of the sample cup. In this spectrometer integrating sphere device, the incident light enters from the integrating sphere entrance window, diffusely reflects with the sample at the light transmission spot row, and the light reflected by the sample exits through the exit window and is received by the detector; the sample cup rotates and drives the component After driving the sample cup to make one rotation, it can complete the spectral scanning of all samples in the sample cup. However, in this integrating sphere device, part of the incident light may be directly reflected from the sample surface to the detector, the collected light is not uniform, the detection result is not accurate enough, and the detection is unstable.

Summary of the invention

In view of the above problems, the present invention provides an integrating sphere detection device, which has better detection stability.

To achieve the above objectives, the technical solutions adopted by the present invention are:

An integrating sphere detection device includes a casing, a liner provided in the casing, a light source and a light sensor provided on the casing, a spherical detection cavity is provided in the liner for the light source to emit The light enters the entrance of the detection cavity, the exit port for the emitted light to irradiate the film to be detected, and the diffuse port for the light reflected by the detection cavity to hit the detection port of the optical sensor, the integrating sphere detection The device further includes a light shielding plate for preventing the outgoing light rays from being directly reflected to the photosensor through the detection film, and the light shielding plate is disposed in the detection cavity and is located between the exit opening and the detection opening.

Further, the shading plate extends inward from the inner wall of the detection cavity in the radial direction of the detection cavity.

Further, a first mounting hole corresponding to the incident port is opened on the housing, and the light source is disposed in the first mounting hole.

Furthermore, the number of the first mounting holes and the number of the entrance ports are both multiple, and each of the first mounting holes corresponds to one of the entrance ports and communicates with each other, and the aperture diameters of the multiple mounting holes Different from each other, the light source is set in one of the first mounting holes during detection, and the other first mounting holes are closed by a plug.

Furthermore, an angle of less than 45 degrees is formed between the center line of any first mounting hole and the center line of the exit port, and the center line of each first mounting hole and the center of the exit port The angle formed between the lines is equal.

Further, a second mounting hole corresponding to the detection port is opened in the casing, and the optical sensor is disposed in the second mounting hole and directly faces the detection port.

Furthermore, the number of the second mounting holes and the number of the detection ports are both plural, and each of the second mounting holes corresponds to one of the detection ports and communicates with each other, and each of the second mounting holes The light sensors are respectively provided; or only part of the second mounting holes are provided with the light sensors and the other second mounting holes are closed by a reflective plate.

Furthermore, the light shielding plate is provided between each detection port and the exit port.

Further, the photo sensor is a photo battery, and the detection signal of the photo battery is transmitted to a current amplifier in real time through a wire.

Further, the entrance port is provided at the upper part of the liner, the exit port is provided at the lower part of the liner, the detection port is provided at the side of the liner; the light source is provided at the In the upper part of the casing, the light sensor is arranged beside the liner.

Further, the light source is a laser light source.

Further, the inner tank is made of polytetrafluoroethylene.

The present invention adopts the above scheme, and has the following advantages over the prior art:

Through the design of the shading plate, etc., the light is directly reflected from the detected film into the light sensor, reducing the impact of the directly reflected light with a large energy level on the detection result of the light sensor, the detection result is accurate, and the detection sensitivity is improved. Weak changes can also be detected; the light source and light sensor are integrated on the housing, the structure is simple and can reduce the impact of assembly errors on the detection results.

BRIEF DESCRIPTION

In order to more clearly explain the technical solutions of the present invention, the drawings required for the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. Ordinary technicians can obtain other drawings based on these drawings without paying any creative labor.

1 is a perspective schematic view of an integrating sphere detection device according to the present invention;

2 is a cross-sectional view of the integrating sphere detection device in FIG. 1 along the vertical direction;

[Correction according to Rule 91 06.01.2020]
3 is a cross-sectional view of the integrating sphere detection device in FIG. 1 along the horizontal direction;

4 is a schematic diagram of detecting the reflected light at any point B on the spherical surface of the cavity.

In the above drawings,

1. Shell; 11, upper cover; 110, first mounting hole; 12, cylinder; 120, second mounting hole; 13, pressure plate; 2, liner; 20, detection cavity; 21, entrance; 22, exit Mouth; 23, detection port; 3. light source; 31, plug; 4, light sensor; 41, wire; 5, shading plate; 6, detected film; 7, reflective plate.

detailed description

The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art. It should be noted here that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation on the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined as long as they do not conflict with each other.

The directional terms such as upper and lower mentioned in the present invention are defined according to the conventional observation angle of those skilled in the art and are convenient for description, and do not limit specific directions. For example, it corresponds to the upper and lower sides of the paper surface in FIG. 2 respectively.

This embodiment provides an integrating sphere detection device according to the present invention, specifically an integrating sphere detection device of an immunodetection analyzer. Referring to FIGS. 1 to 3, the integrating sphere detection device housing 1, a liner 2 provided in the housing 1, a light source 3 and a light sensor 4 provided on the housing 1, a spherical detection cavity is provided in the liner 2 20. The outgoing light from the light source 3 enters the entrance 21 of the detection cavity 20, the outgoing port 22 from which the outgoing light irradiates the sample, and the outgoing light from the detection cavity 20 to the detection port 23 of the optical sensor 4. The integrating sphere detection device further includes a light-shielding plate 5 for preventing the reflected light from directly reflecting to the light sensor 4 through the sample (the detection film 6). The light-shielding plate 5 is disposed in the detection cavity 20 and is located between the exit port 22 and the detection port 23 In the meantime, its function is mainly to block light and prevent direct reflection to the light sensor 4. The light-shielding plate 5 extends inward from the inner wall of the detection chamber 20 in the radial direction of the detection chamber 20. Preferably, the light-shielding plate 5 has a substantially flat plate shape, and extends substantially in the radial direction of the spherical detection cavity 20. The light source 3 is specifically a laser light source, and the film to be detected 6 is specifically a cellulose film.

The entrance port 21 is provided at the upper part of the liner 2, the exit port 22 is provided at the lower part of the liner 2, the detection port 23 is provided at the side of the liner 2, the light source 3 is provided at the upper part of the housing 1, and the light sensor 4 is provided at the liner 2 on the side. Specifically in this embodiment, the housing 1 is composed of an upper cover 11 and a cylinder 12, the cylinder 12 is generally cylindrical and the upper end is open, the cylinder 12 has a cylindrical cavity for accommodating the liner 2, the upper The cover 11 is fixedly connected to the upper end of the barrel 12 to seal the liner 2 in the barrel 12. The housing 1 is made of metal or metal alloy, such as aluminum; the liner 2 is made of polytetrafluoroethylene (F4) in one piece.

A first mounting hole 110 corresponding to the entrance 21 is opened on the upper cover 11 of the housing 1, and the light source 3 is disposed in the first mounting hole 110 and directly faces the entrance 21. Preferably, both the number of the first mounting holes 110 and the number of the entrances 21 are multiple, each first mounting hole 110 corresponds to one entrance 21 and communicates with each other, and the diameters of the multiple first mounting holes 110 are different from each other, During detection, the light source 3 is disposed in one of the first mounting holes 110, and the other first mounting holes 110 are closed by a plug.

A second mounting hole 120 corresponding to the detection port 23 is opened on the side wall of the cylindrical body 12 of the housing 1, and the optical sensor 4 is disposed in the second mounting hole 120 and directly faces the detection port 23. Preferably, the number of the second mounting holes 120 and the number of the detection ports 23 are both multiple, and each second mounting hole 120 corresponds to one detection port 23 and communicates with each other, and each second mounting hole 120 is provided with a photo sensor 4 Or, only part of the second mounting hole 120 is provided with the optical sensor 4 and other second mounting holes 120 are closed by the reflective plate 7. In addition, a light shielding plate 5 is provided between each detection port 23 and the exit port 22. After the optical sensor 4 is installed in the second mounting hole 120, the optical sensor 4 is fixed in the second mounting hole 120 through the pressing plate 13. The pressure plate 13 is fixedly installed on the side wall of the barrel 12 by screws, and the pressure plate 13 is provided with two small holes through which the wires 41 of the optical sensor 4 pass. When the optical sensor 4 is not installed in a second mounting hole 120, the corresponding detection port 23 is closed by a reflective plate 7, the role of the reflective plate 7 is to complement the inner surface of the detection cavity 20, reflect light, and the reflective plate 7 Uses the same material as liner 2.

The bottom wall of the outer cylinder 12 is provided with a through hole at a position corresponding to the outlet 22, and the lower portion of the liner 2 has a ring of flanges extending into the through hole. The wall of the through hole surrounds the flange and protrudes. The exit port 22 is formed between the edges.

The photo sensor 4 is specifically a photo battery, and the detection signal of the photo battery is transmitted to a current amplifier in real time through the wire 41.

An angle of less than 45 degrees is formed between the center line of any first mounting hole 110 and the center line of the outlet 22, and the center line of each first mounting hole 110 is formed between the center line of the outlet 22 The included angle is substantially equal, preferably less than 10 degrees, specifically 8 degrees in FIG. 2, which can reduce the total reflection light back to the light source 3 and avoid damage to the laser light source. Specifically, in the above integrating sphere detection device, the center line of the first mounting hole 110 and the center line of the corresponding entrance 21 coincide with each other (or parallel), and the center line of the second mounting hole 120 and the center of the corresponding detection port 23 The lines coincide with each other (or parallel), and the center line of the outlet 22 passes through the center of the sphere of the detection cavity 20. Therefore, the first mounting holes 110 are inclined holes that are offset from the outlet 22 by a distance. Further, the first mounting holes 110 are provided in pairs, as shown in the two first mounting holes 110 shown in FIG. 1, the two first mounting holes 110 are substantially symmetrical with respect to the exit 22, therefore, when one of the first mounting holes The light beam emitted by the laser light source in 110 enters another first mounting hole 110 through the reflected light reflected by the detection film 6 at the exit port 22, and passes through the first mounting hole 110 (where a plug 31 is provided to form a blind hole ) Absorb the reflected beam. On the one hand, the directly reflected light is directly absorbed by the first mounting hole 110 without entering the detection port 23; on the other hand, the light emitted from the laser light source 3 is prevented from being directly reflected into the light source 3 and causing damage to the light source.

Referring to FIG. 2, at the time of detection, the light source 3 is installed in the first mounting hole 110 of the corresponding specification, and the film to be detected 6 is placed under the exit port 22, and the light beam emitted from the light source 3 is irradiated on the film to be detected. The detected film 6 is reflected to the inner wall of the spherical detection cavity 20 and the light shielding plate 5, and is reflected multiple times by the spherical detection cavity 20 and the light shielding plate 5 to form uniform diffuse emission light in the spherical detection cavity 20, and the diffuse reflection light passes through the detection port 23 is collected by the photovoltaic cell, and the photovoltaic cell generates a current of a corresponding magnitude according to the collected diffuse reflection light, and outputs it to a current amplifier through the wire 41 to realize detection. The integrating sphere detection device is particularly suitable for immunodetection using a porous substance as a carrier, such as a cellulose membrane.

The detection cavity 20 is a spherical inner cavity, and the inner wall of the detection cavity 20 is coated with a random reflection coating, and the random reflection at each point of the inner wall of the detection cavity is uniform. The reflectivity of the scattered reflective layer is ρ, the spherical radius of the detection cavity 20 is R, and the total flux of the light source is Φ. As shown in FIG. 4, the illuminance E of the light source S at any point B on the spherical surface of the detection cavity 20 is the sum of repeated irradiation of scattered reflected light, as shown in the following formula (1):

Among them, E1 is the illuminance directly illuminating from the light source S to point B;

In the above integrating sphere detection device, the light beam directly hitting the point B is blocked by the shading plate, then E1=0, and the illuminance E at any point B on the spherical surface of the detection cavity 20 is as shown in the following formula (2):

Therefore, the integrating sphere detection device of the present invention blocks the light directly reflected by the detection film 6 from entering the detection port 23 through the design of the shading plate 5 and the first mounting hole 110, so that E1 in the above formula (1) is approximately zero The light illuminance E detected by the light sensor 4 through the detection port 23 is as shown in the above formula (2), that is, the light detected by the light sensor 4 entering the detection port 23 is basically diffuse reflection light. Since the directly reflected light beam and the diffusely reflected light beam are not in the same energy level, the energy of the former is much greater than the energy of the latter. If the light detected by the light sensor contains the part directly reflected by the detection film, then in formula (1), The value of E1 is much larger than the latter, so the change of diffuse reflected light has little effect on the overall illuminance E, and is not easy or even detectable. In the present invention, through the design of the shading plate and the first mounting hole, the detection value of the light sensor is only affected by the diffuse reflection light, highlighting the diffuse reflection part, and the weak change of the diffuse reflection light can also be detected by the light sensor, thereby improving Detection sensitivity.

The above integrating sphere detection device has the following characteristics:

Through the design of the shading plate 5 and the plurality of first mounting holes 110, the light is directly reflected from the detected film 6 into the light sensor 4, reducing the impact of the directly reflected light with a larger energy level on the detection result of the light sensor 4, and the detection result is accurate , Improves the detection sensitivity, and can detect the weak changes of the diffuse reflected light; the light source 3 and the light sensor 4 are integrated in the mounting holes on the housing, the structure is simple and can reduce the impact of assembly errors on the detection results; Multiple entrances 21 and multiple first mounting holes 110 of different specifications, the light source 3 of the selected specifications can be installed in the corresponding first mounting hole 110, and the other first mounting holes 110 can be closed for flexible use Convenient; and the first mounting hole is an oblique hole, to avoid the total reflection of the emitted light directly reflected to the light source, causing damage to the light source; multiple detection ports 23 and multiple second mounting holes 120 are provided, which can be provided with multiple light sensors 4. It can also detect the weak diffuse light. The integrating sphere detection device is particularly suitable for immunodetection using a porous substance as a carrier, such as a cellulose membrane.

The above-mentioned embodiment is only to illustrate the technical concept and features of the present invention, and is a preferred embodiment. Its purpose is that those familiar with this technology can understand the content of the present invention and implement it accordingly, and cannot limit the scope of the present invention. protected range. All equivalent changes or modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims

An integrating sphere detection device, characterized in that it includes a casing, an inner bladder provided in the casing, a light source and an optical sensor provided in the casing, and a spherical detection cavity is provided in the inner bladder , For the outgoing light of the light source to enter the entrance of the detection cavity, for the outgoing light to irradiate the exit port on the film to be detected, for the light reflected by the detection cavity diffusely to the light The detection port of the optical sensor and the light shielding plate for preventing the outgoing light from directly reflecting to the light sensor through the detection film, the light shielding plate is arranged in the detection cavity and located at the outgoing light Between the mouth and the detection port, and extending inward from the inner wall of the detection cavity in the radial direction of the detection cavity,

The entrance port is located at the upper part of the liner, the exit port is located at the lower part of the liner, the detection port is located at the side of the liner; the light source It is arranged on the upper part of the casing, and the light sensor is arranged on the side of the liner. The casing is provided with a first mounting hole corresponding to the incident port, the The light source is disposed in the first mounting hole, the number of the first mounting hole and the number of the entrance ports are both multiple, and each of the first mounting holes corresponds to one of the entrance ports And communicate with each other. The diameters of a plurality of the mounting holes are different from each other. During the detection, the light source is set in one of the first mounting holes, and the other of the first mounting holes is closed by a plug. An angle of less than 45 degrees is formed between the center line of a first mounting hole and the center line of the outlet, and the center line of each of the first mounting holes and the center line of the outlet The angle formed between them is equal,

The casing is provided with a second mounting hole corresponding to the detection port, the photo sensor is disposed in the second mounting hole and directly faces the detection port, the first The number of the two mounting holes and the number of the detection ports are both multiple, and each of the second mounting holes corresponds to one of the detection ports and communicates with each other, and each of the second mounting holes is provided with The optical sensor, or only part of the second mounting hole is provided with the optical sensor and the other second mounting hole is closed by a reflective plate, each of the detection port and the exit The said shading plates are respectively arranged between the ports.
The integrating sphere detection device according to claim 1, wherein the center line of the first mounting hole and the center line of the corresponding entrance coincide with or parallel to each other, and the center line of the second mounting hole and The center lines of the corresponding detection ports coincide or are parallel to each other, and the center line of the exit port passes through the spherical center of the detection cavity.
An integrating sphere detection device, characterized in that it includes a casing, an inner bladder provided in the casing, a light source and an optical sensor provided in the casing, and a spherical detection cavity is provided in the inner bladder , The light exiting the light source enters the entrance of the detection cavity, the exit light irradiates the exit opening on the film to be detected and the light diffusely reflected by the detection cavity hits the entrance The detection port of the optical sensor, the integrating sphere detection device further includes a light-shielding plate for preventing the outgoing light from directly reflecting to the light sensor through the detection film, the light-shielding plate is provided in the The detection cavity is located between the exit port and the detection port, and extends inward from the inner wall of the detection cavity in the radial direction of the detection cavity.
The integrating sphere detection device according to claim 3, wherein the shading plate extends inward from the inner wall of the detection cavity in the radial direction of the detection cavity.
The integrating sphere detection device according to claim 3, characterized in that: the housing is provided with a first mounting hole corresponding to the incident port, and the light source is disposed on the first mounting hole Inside the hole.
The integrating sphere detection device according to claim 5, wherein the number of the first mounting holes and the number of the entrance ports are both multiple, and each of the first mounting holes corresponds to a The incident ports are in communication with each other, the diameters of the plurality of mounting holes are different from each other, and the light source is set in one of the first mounting holes during detection, and the other of the first mounting holes passes through the plug Closed.
The integrating sphere detection device according to claim 6, characterized in that an angle of less than 45 degrees is formed between the center line of any of the first mounting holes and the center line of the exit port, and each The angle formed between the center line of the first mounting hole and the center line of the exit port is equal.
The integrating sphere detection device according to claim 3, characterized in that: a second mounting hole corresponding to the detection port is opened in the casing, and the optical sensor is provided in the second Install in the hole and face the detection port.
The integrating sphere detection device according to claim 8, wherein the number of the second mounting holes and the number of the detection ports are both plural, and each of the second mounting holes corresponds to a The detection ports are in communication with each other. Each of the second mounting holes is provided with the optical sensor, or only a portion of the second mounting hole is provided with the optical sensor and the other of the first The two mounting holes are closed by the reflective plate.
The integrating sphere detection device according to claim 9, wherein each of the detection port and the exit port is provided with the light shielding plate.
The integrating sphere detection device according to claim 3, wherein the entrance port is provided at the upper part of the liner, the exit port is provided at the lower part of the liner, and the detection The mouth is provided on the side of the inner bladder; the light source is provided on the upper portion of the housing, and the light sensor is provided on the side of the inner bladder.
The integrating sphere detection device according to claim 3, wherein the casing is composed of an upper cover and a cylinder, the cylinder is generally cylindrical and the upper end is open, and the cylinder has A cylindrical cavity for accommodating the inner bladder, the upper cover is fixedly connected to the upper end of the barrel to close the inner bladder in the barrel.
The integrating sphere detection device according to claim 3, wherein the bottom wall of the outer cylinder is provided with a through hole at a position corresponding to the exit port, and the lower portion of the inner tank has A ring of flanges extending into the through hole, the hole wall of the through hole surrounding the flange, and the exit opening is formed between the flanges.
The integrating sphere detection device according to claim 3, wherein the inner wall of the detection cavity is coated with a random reflection coating, and the random reflection of each point of the inner wall of the detection cavity is uniform.
The integrating sphere detection device according to claim 3, wherein the photo sensor is a photo battery, and the detection signal of the photo battery is transmitted to a current amplifier in real time through a wire.
The integrating sphere detection device according to claim 3, wherein the light source is a laser light source.
The integrating sphere detection device according to claim 3, wherein the housing is made of metal or metal alloy.
The integrating sphere detection device according to claim 3, wherein the inner liner is made of polytetrafluoroethylene.