WO2020082148A1 - Composição biológica de funções múltiplas - Google Patents

Composição biológica de funções múltiplas Download PDF

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Publication number
WO2020082148A1
WO2020082148A1 PCT/BR2019/050459 BR2019050459W WO2020082148A1 WO 2020082148 A1 WO2020082148 A1 WO 2020082148A1 BR 2019050459 W BR2019050459 W BR 2019050459W WO 2020082148 A1 WO2020082148 A1 WO 2020082148A1
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Prior art keywords
methyl
carboxamide
pyrazole
spp
bacillus
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PCT/BR2019/050459
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English (en)
French (fr)
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Eduardo Roberto de Almeida BERNARDO
Thales Facanali MARTINS
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Agrivalle Brasil Industria E Comércio De Produtos Agrícolas Ltda
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Priority to US17/285,247 priority Critical patent/US20210368808A1/en
Priority to EP19876144.7A priority patent/EP3872167A4/en
Publication of WO2020082148A1 publication Critical patent/WO2020082148A1/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/38—Trichoderma
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P15/00—Biocides for specific purposes not provided for in groups A01P1/00 - A01P13/00
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • This patent is the invention of biological compositions of multiple functions for biological control of phytopathogens, phytomatodes and agricultural pests, inoculant, phosphorus solubilization, soil conditioning, biostimulant, reduction of water stress in cultivated plants and bioremediation in arable areas or not, more particularly these are compositions containing mixtures of additives and excipients to at least 2 (two) genera and / or species of Actinomycetes, Actinomycetes in mixtures with representatives of the genera of Bacillus, Actinomycetes in mixtures with fungi of the Orders Hypocreales, Helotiales and Mucorales and a combination of them or part of them, with nematicidal effects on damage reduction and control of phytomatodes, with fungicidal effects on harm reduction and control, with insecticidal action on harm reduction and control, with action to promote growth, soil conditioner, biostimulants, in the mitigation of water stress in cultivated plants and bioremediation in agricultural or non-agricultural areas
  • Actinomycetes comprise more than 30% of the population of soil microorganisms (Kennedy, 1999). These microorganisms are metabolically diverse and are capable of using a variety of energy sources, and may also be autotrophic, heterotrophic, chemotrophic or phototrophic (Kennedy, 1999). They play a crucial role in the decomposition of polluting organic compounds active in nature (Groth et al., 1999). They are also active in the decomposition of organic materials in the soil, including lignin and other recalcitrant polymers and can degrade agricultural and urban waste (Hauer, et al., 1997).
  • Actinomycetes are qualitatively and quantitatively important in the rhizosphere. According to Crawford et al. (1993), they can influence the growth of plants and protect the roots from the invasion of phytopagenic organisms, being able to be used as biological control agents. They act as antagonistic agents for fungi, bacteria and phytomatodes or producing fungicidal, bactericidal and nematicidal compounds. Pandhare et al. (2002), verified the production and inhibitors of alkaline protease by Streptomyces sp. This fact is very important because these proteins are essential for the life cycle of many phytopathogenic organisms, and can therefore be used in biological control in plants.
  • Actinomycetes also produce chemicals such as thiamine, riboflavin, vitamin B12, various porphyrins, compounds containing iron and coenzymes that can promote or inhibit the growth of other organisms (Kennedy, 1999).
  • microorganisms are important degraders of pesticides. This degradation capacity is not restricted to one gender or family. The main ones belong to the suborder genera: Micrococcineae (Arthrobacter, Brevibacteriu, Clavibacter), Corynebacterineae
  • Actinomycetes can degrade several pesticides, such as organochlorines, symmetrical triazines, triazinones, carbamates, sulfonylureas and acetanilides. Degradation can occur through the use of these compounds as the only source of carbon and energy by cometabolisms (Schrijver & Mot, 1999). In studies conducted by Esposito et al. (1998), Streptomyces showed good potential for bioremediation of soils contaminated with Diuron, one of the most widely used herbicides in Brazil, without producing secondary metabolites.
  • Streptomyces have the capacity to produce large amounts of enzymes with varied industrial possibilities.
  • the main groups of enzymes are oxidoreductases, transferases, hydrolases, lyases, isomerases and synthases. In nature, they play an important role in the formation of humus, especially for the production of extracellular enzymes capable of degrading cellulolytic compounds (Padilha, 1998). In addition, they are important producers of antibiotics.
  • Nocardia and Rhodococcus are important components of soil microflora and are involved in the degradation of organic matter and are capable of decomposing environmentally harmful chemical compounds (Groth et al., 1999).
  • antagonism and antibiosis tend to be the predominant mode of action of bacteria against phytonematodes and fungi.
  • these nematicidal compounds can have a direct action on the hatching of eggs or on mobility, and indirect effects, such as changes in root exudates, confusion in locomotion or resistance induction are also observed (Sikora & Hoffmann-Hergarten , 1992; Hasky-Ginther et al. 1998).
  • the use of bacteria as agents of biological control has shown to be a promising alternative in the productive system (Hallmann et al.
  • Bacillus spp. The genus Bacillus spp. is recognized as an excellent producer of antimicrobial compounds (Huang & Chang, 1975; Baker et al., 1983; Motomura & Hirooka, 1996) and the finding of the production of substances that inhibit phytopathogens, produced by several isolates of Bacillus spp., is especially important for understanding the mechanisms of action of these microorganisms for antagonism.
  • Antagonistic bacteria like B. subtilis, generally act significantly by antibiosis and, occasionally, by parasitism and competition. In this way, the disease control can be effective due to the substances produced and, not only due to the mass introduction of biocontrol agents (Arras & Arru, 1997).
  • Microorganisms that act by antibiosis usually have a wide spectrum of action, so that in the inhibition of fungi the production of toxic substances is more effective than any other mechanism of action involved.
  • RPCP plant growth promoting rhizobacteria
  • the objective of the present invention is to provide effective compositions for the reduction of damages caused by phytopathogens, phytomatomatodes and agricultural pests, to promote plant growth, to condition soils and to mitigate damages caused by eventual water stresses or environmental contamination in spraying, spraying of planting furrow, drench, in the covering (industrial or not), treatment (industrial or not) and incrustation (industrial or not) of seeds in addition to polymers / excipients for application in cultivated plants, not limited to Coffea spp (Coffea arabica, Coffea canefora, Coffea robusta), Brachiaria spp.
  • Coffea spp Coffea arabica, Coffea canefora, Coffea robusta
  • the present invention relates to biological compositions of multiple functions, for spraying, furrow spraying, drench, coating (industrial or not), treatment (industrial or not) and incrustation (industrial or not) of seeds of cultivated plants , containing mixtures of at least 2 (two) genera and / or species of Actinomycetes or their mutants, Actinomycetes in mixtures with Bacillus or its mutants, Actinomycetes in mixtures with fungi or their mutants and combination of them or part of them, concomitantly with pesticides (or not), additives and excipients, in biological compositions with fungicidal, nematicidal properties, growth-stimulating insecticides, soil conditioners and reduction of water stress effects in cultivated plants and bioremediation and cultivated areas.
  • the biological compositions of multiple functions comprise: 1.0 to 20.0% of at least 2 (two) genera and / or species of: Actinomycetes and their mutants; Actinomycetes in mixtures with representatives of the Bacillus genera; Actinomycetes in mixtures with fungi of the Orders Hypocreales, Orbiliales, Helotiales and Mucorales, and combination between them or part of them (in colony forming units, u.f.c.);
  • Actinomycetes and their mutants belong to the group consisting of Streptomyces spp., Artrobacter spp., Brevibacterium spp., Clavibacter spp., Corynebacterium spp., Nocardia spp, Rhodococcus spp., Micromonospora spp, Nocardioides spp. and Frankia spp ..
  • Actinomycetes in mixtures with representatives of the Bacillus genera belong to the group consisting of Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus kustaki, Bacillus lactosa, Bacillus lacticola , Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nigrificans, Bacillus popilliae, pumilus, Bacillus siamensis, Bacillus subtilis, Bacilus lichenifillis, Bacillus lichenifillis, Bacillus . .
  • Actinomycetes in mixtures with fungi of the Hypocreable Orders belong to the group consisting of the species of Trichoderma amazonicum, Trichoderma asperellum, Trichoderma atroviride, Trichoderma aureoviride, Trichoderma austrokoningii, Trichoderma brevicompactum, Trichoderma cinnamumoning, Trichoderma cinnamome Trichoderma longibrachiatum, Trichoderma polysporum, Trichoderma pseudokoningii, Trichoderma reesei, Trichoderma stromaticum, virens Trichoderma, Trichoderma viride, Trichoderma viridescens, Clonostachys agarwalii, asymmetrica Clonostachys, Clonostachys aurantia, Clonostachys chlorina, compact Clonostachys, cylindrospora Clonostachys, farinosa Clonostachys, Clo
  • Actinomycetes in mixtures with Orbiliales belong to the group consisting of Arthrobotrys oligospora, Arthrobotrys aggregata, Arthrobotrys alaskana, Arthrobotrys amerospora, Arthrobotrys botryospora, Arthrobotrys brochopaga, Arthrobotrys conentes, Arthrobotrys congorges, Arthrobotrys, Arthrobotrysotros, Arthrobotrysotros ellipsospora, Arthrobotrys entomopaga, Arthrobotrys globospora, Irregular Arthrobotrys, Arthrobotrys javanica, Arthrobotrys longispora, Arthrobotrys musiformis, Arthrobotrys nematopaga, Arthrobotrys oligospora.
  • Actinomycetes in mixtures with Helotiales belong to the group consisting of the species of Monacrosporium robustum, Monacrosporium appendiculatum, Monacrosporium ellipsosporum Monacrosporium eudermatum, Monacrosporium fusiforme, Monacrosporium globosporum, Monacrosporium leptosporum leptosporum.
  • Actinomycetes in mixtures with Mucorales belong to the group consisting of the species of Cunninghamella elegans.
  • Additives are dispersants selected from the group consisting of water-soluble ionic polymers, water-soluble anionic polymers, surfactants selected from the group consisting of anionic surfactants and non-ionic surfactants and combinations between them.
  • excipients belong to the group consisting of: silicas, talc, bentonite, carbohydrates, carbonates, casein, whey and milk products and combinations between them.
  • Pesticides consist of: a) Insecticides - capsaicin, cartap, celastrus-extract, chlorantraniliprole, chlorbenzuron, chlorethoxyfos, chlorfluazuron, chlorpyrifos-e, cnidiadin, cryolite, cyanophos, cyantraniliprole, cyclaniliprole, cyhalothrin, cyhexatin, cypermethrin, dacnusa, dcip, dichloropropene, dicofol, diglyphus, diglyphus + dacnusa, dimethacarb, dithioether, dodecylacetate, emamectin, fatty, euty, euty, eutoc, euty acids / salts, fenazaquin, fenobucarb (BPMC), fen pyroxi mate, flubro
  • Herbicides alloxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tralkoxydim, butroxydim, clefoxydim, tepraloxydim; phenoxyphenoxypropionic esters, clodinafop-propargyl, cyhalofop-butyl, diclofop-methyl, fenoxa prop-ethyl, fenoxaprop-P-ethyl, fenthiapropethyl, fluazifop-butyl, fluazifop-P-butyl, haloxyfop-ethoxyethyl, haloxyfop-haloxyop methyl, isoxapyrifop, propaquizafop, quizalofop-ethyl, quizalofop-P-ethyl or quizalofop-tefuryl,
  • esprocarb molinate, pebulate, prosulfocarb, thiobencarb, tri-allate, vemolate; benfuresate, perfluidone; asulam, carbetamid, chlorpropham, orbencarb, pronamid, propham, tiocarbazil benefin, butralin, dinitramin, ethalfluralin, fluchloralin, oryzalin, pendimethalin, prodiamine, trifluralin dithiopyr, thiazopyr, butififen, acylif, acid, chifort , bifenox, chlomitrofen (CNP), ethoxyfen, fluorodifen, fluoroglycof enethyl, fomesafen, furyloxyfen, lactofen, nitrofen, nitrofluorfen or oxyfluorfen, oxadiargyl, oxadia
  • compositions must be used as a wettable powder formulation.
  • other formulations containing these microorganisms such as emulsions, microemulsions, suspensions, concentrated suspensions, granules, encapsulation, microencapsulation, nanoencapsulation, among others, can also be used.
  • compositions of multiple functions are for biological control of phytopathogens, phytomatodes and agricultural pests, inoculant, phosphorus solubilization, soil conditioning, biostimulant, reduction of water stress in cultivated plants and bioremediation in agricultural areas or not with nematicidal effects in the reduction of damage and control of phytomatodes, not limited to Pratylenchus brachyurus, Pratylenchus zea, Meloidogyne incognita, Meloidogyne javanica, Meloidogyne exigua, Heterodera glycines, Rotylenchulus reniformis, Pratylenchus coffea, Helicotilechus dihystera, fungicidal effects in harm reduction and control, not limited to Phakopsora pachyrhizi, Corynespora cassucola, Puccinia polysora, Exserohilum turcicum, Xc
  • the composition may have the following concentrations:
  • Streptomyces flavovirences 1.0 to 20.0%
  • Nocardia erythropolis 1.0 to 20.0%
  • Streptomyces grisus 1.0 to 20.0%
  • Bacillus licheniformis 1.0 to 20.0%
  • Bacillus amyloliquefaciens 1.0 to 20.0%
  • Nocardia asteroids 1.0 to 20.0%
  • Bacillus licheniformis 1.0 to 15.0%
  • Trichoderma harzianum 1.0 to 20.0%
  • Purpuriocillium lilacinus 1.0 to 15.0%
  • Streptomyces purpuracens 1.0 to 20.0%
  • a composition containing 20.0% Streptomyces flavovirences, 20.0% Nocardia erythropolis, 3.0% acrylic styrene polymer, 1.0% anionic surfactant and 56.0% inert was formulated to evaluate its efficiency in the control of phytonematodes, phytopathogens and biostimulation.
  • a composition containing 20.0% Streptomyces grisus, 7.0% Bacillus licheniformis, 7% Bacillus amyloliquefaciens, 3.0% acrylic styrene polymer, 1.0% anionic surfactant, 10.0% azoxystrobin and 52.0% inert was formulated to evaluate its efficiency in the control of phytopathogens, stimulating leaf and root growth and reducing water stress.
  • a composition containing 20.0% Nocardia asteroides, 7.0% Bacillus licheniformis, 20.0% Trichoderma harzianum, 10.0% Purpuriocillium lilacinus, 3.0% acrylic styrene polymer, 1, 0% anionic surfactant and 39.0% inert was formulated to assess its efficiency in controlling phytopathogens, stimulating leaf and root growth and reducing water stress.
  • a composition containing 20.0% Streptomyces purpuracens, 7.0% Bacillus thuringiensis, 20.0% Beauveria bassiana, 3.0% acrylic styrene polymer, 1.0% anionic surfactant and 49.0 % of inert was formulated to evaluate its efficiency in insect control.
  • compositions for the biological control of phytopathogens, phytomatodes and insects, inoculants, soil conditioners, biostimulants, water stress reducers in plants and bioremediation should follow the following flow of events:
  • Raw material the raw materials that will compose the product must be received and handled by trained people
  • the present invention can be applied either by spraying via bar, spraying via planting furrow, drench, or coating (industrial or not), treatment (industrial or not) and incrustation (industrial or not) of seeds of plants grown for the biological control of phytopathogens, phytomatodes and insects, inoculants, soil conditioners, biostimulants and water stress reducers for cultivated plants and bioremediation.
  • the application method must be analyzed on a case-by-case basis and will depend on the technical conditions and needs of each producer.
  • compositions for the biological control of phytopathogens, phytomatodes and insects, inoculants, soil conditioners, biostimulants and water stress reducers for cultivated plants and bioremediation containing mixtures of Actinomycetes or Actinomycetes in mixtures with bacteria and / or fungi offer the following advantages:
  • Coffea spp Coffea arabica, Coffea canefora, Coffea robusta
  • Brachiaria spp. Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Virology (AREA)
  • Dentistry (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

Trata-se a presente patente de invenção de composições biológicas de funções múltiplas para o controle biológico fitopatógenos, fitonematóides e pragas agrícolas, inoculante, solubilização de fósforo, condicionamento de solos, bioestimulante, redução de estresse hídrico em plantas cultivadas e biorremediação em áreas agricultáveis ou não, mais particularmente trata-se de composições contendo misturas de aditivos e excipientes a pelo menos 2 (dois) gêneros e/ou espécies de Actinomicetos, Actinomicetos em misturas com representantes dos gêneros de Bacillus, Actinomicetos em misturas com fungos das Ordens Hypocreales, Helotiales e Mucorales e combinação entre eles ou parte deles, com efeitos nematicidas na redução dos danos e controle de fitonematóides, com efeitos fungicidas na redução dos danos e controle fitopatógenos, com ação inseticida na redução dos danos e controle de insetos, com ação de promoção de crescimento, condicionador de solos, bioestimulantes, na mitigação do estresse hídrico das plantas cultivadas e biorremediação em áreas agricultáveis ou não, em adição aos defensivos agrícolas (ou não), polímeros/excipientes em formulações para pulverização, pulverização de sulco de plantio, drench, revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas. Pertencente ao setor técnico de biotecnologia, essa composição amplia a eficiência na condução das plantas cultivadas por utilizar mecanismos de ação diferentes e complementares das composições dos microrganismos destacadas acima.

Description

COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES MÚLTIPLAS
[001] Trata-se a presente patente de invenção de composições biológicas de funções múltiplas para o controle biológico fitopatógenos, fitonematóides e pragas agrícolas, inoculante, solubilização de fósforo, condicionamento de solos, bioestimulante, redução de estresse hídrico em plantas cultivadas e biorremediação em áreas agricultáveis ou não, mais particularmente trata-se de composições contendo misturas de aditivos e excipientes a pelo menos 2 (dois) gêneros e/ou espécies de Actinomicetos, Actinomicetos em misturas com representantes dos gêneros de Bacillus, Actinomicetos em misturas com fungos das Ordens Hypocreales, Helotiales e Mucorales e combinação entre eles ou parte deles, com efeitos nematicidas na redução dos danos e controle de fitonematóides, com efeitos fungicidas na redução dos danos e controle, com ação inseticida na redução dos danos e controle, com ação de promoção de crescimento, condicionador de solos, bioestimulantes, na mitigação do estresse hídrico das plantas cultivadas e biorremediação em áreas agricultáveis ou não, em adição a defensivos agrícolas (ou não), polímeros/excipientes em formulações para pulverização, pulverização de sulco de plantio, drench, revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas. Pertencente ao setor técnico de biotecnologia, essa composição amplia a eficiência na condução das plantas cultivadas por utilizar mecanismos de ação diferentes e complementares das composições dos microrganismos destacadas acima.
ESTADO DA TÉCNICA
[002] No atual cenário agrícola mundial, os ganhos de produção têm sido, muitas vezes, vinculados a aumentos gradativos de produtividade sem que ocorra um aumento na área agricultável. Esses aumentos de produtividade vêm sendo obtidos por meio de avanços significativos nas técnicas de cultivo, utilização de variedades mais adaptadas a fatores bióticos e abióticos, adequação do requerimento nutricional das plantas e, também, pelo melhor controle e redução dos danos causados por agentes fitopatogênicos e pragas agrícolas. Dentre esses avanços, o controle de pragas e doenças ainda é visto como o maior desafio na manutenção da produtividade das culturas. Diversas técnicas podem ser empregadas, com maior ou menor grau de eficácia, mas, de maneira geral, o uso de defensivos químicos continua sendo o método mais utilizado.
[003] Entretanto, para o controle de fitopatógenos, fitonematóides e pragas agrícolas, o uso de defensivos químicos tem mostrado, muitas vezes, resultados insatisfatórios. A maior parte desse problema decorre do uso indiscriminado e excessivo de moléculas químicas, que, frequentemente tem ocasionado problemas de intoxicação humana e/ou animal, concomitantemente a contaminação do meio ambiente. Esses eventos têm levado a um aumento da percepção pública contrário ao uso de defensivos químicos e à busca incessante por técnicas de manejo mais seguras ou alternativas.
[004] Assim, controles alternativos para fitonematoides, fitopatógenos e pragas agrícolas vêm sendo empregados sempre que possível, mas, adversidades inerentes à biologia desses organismos, costumam inviabilizar o uso de algumas técnicas. Por exemplo, variedades de cultivares resistentes são raramente disponíveis, enquanto que a rotação de cultura costuma ser inviável, devido aos custos económicos ou a grande gama de hospedeiros para algumas espécies (Bird et al., 2003)
[005] Nesse contexto, o controle biológico utilizando microrganismos vem sendo considerado uma opção viável, tanto para tratamento de sementes, tratamento de sulco de plantio ou pulverização das culturas de interesse.
[006] Os Actinomicetos compreendem mais de 30% da população de microrganismos do solo (Kennedy, 1999). Esses microrganismos são metabolicamente diversos e são capazes de utilizar variadas fontes de energia, podendo ainda ser autotróficos, heterotróficos, quimiotróficos ou fototróficos (Kennedy, 1999). Desempenham um papel crucial na decomposição de compostos orgânicos poluentes ativos na natureza (Groth et al., 1999). São ativos também na decomposição de materiais orgânicos no solo, incluindo lignina e outros polímeros recalcitrantes e podem degradar resíduos agrícolas e urbanos (Hauer, et al., 1997).
[007] Os Actinomicetos são qualitativa e quantitativamente importantes na rizosfera. Segundo Crawford et al. (1993), eles podem influenciar no crescimento de plantas e proteger as raízes da invasão de organismos fitopagênicos, podendo ser utilizados como agentes de controle biológico. Atuam como agentes antagonistas de fungos, bactérias e fitonematóides ou produzindo compostos fungicidas, bactericidas e nematicidas. Pandhare et al. (2002), verificaram a produção e inibidores de protease alcalina por Streptomyces sp. Este fato é bastante importante porque estas proteínas são essenciais para o ciclo de vida de muitos organismos fitopatogênicos, podendo dessa forma, serem utilizados no controle biológico em plantas.
[008] Os Actinomicetos também produzem produtos químicos como a tiamina, riboflavina, vitamina B12, várias porfirinas, compostos contendo ferro e coenzimas que podem promover ou inibir o crescimento de outros organismos (Kennedy, 1999).
[009] Estes microrganismos são importantes degradadores de pesticidas. Essa capacidade de degradação não está restrita a um gênero ou família. Os principais pertencem aos gêneros das subordens: Micrococcineae ( Arthrobacter , Brevibacteriu, Clavibacter ), Corynebacterineae
0 Corynebacterium , Nocardia, Rhodococcus ), Micromonosporineae
( Micromonospora ), Propionibacterineae ( Nocardioides ) e Strepetomycineae (, Streptomyces ). Estes Actinomicetos podem degradar diversos pesticidas, tais como organoclorados, triazinas simétricas, triazinonas, carbamatos, sulfonilúreas e acetanilidas. A degradação pode ocorrer através da utilização destes compostos como única fonte de carbono e energia pelo cometabolismos (Schrijver & Mot, 1999). Em trabalhos conduzidos por Esposito et al. (1998), Streptomyces apresentou bom potencial para biorremediação de solos contaminados com Diuron, um dos herbicidas mais utilizados no Brasil, sem produção de metabólitos secundários.
[010] Streptomyces possuem capacidade de produzir grandes quantidades de enzimas com variadas possibilidades industriais. Os principais grupos de enzimas são as oxidoredutases, transferases, hidrolases, liases, isomerases e sintases. Na natureza, desempenham um papel importante na formação do húmus, especialmente pela produção de enzimas extracelulares com capacidade de degradar compostos celulolíticos (Padilha, 1998). Além disso, são importantes produtores de antibióticos.
[011] Membros do gênero Frankia fixam nitrogénio em nódulos de plantas não leguminosas (Heuer et al., 1997). Por esta razão são importantes para o balanço mundial de nitrogénio (Araújo, 1998).
[012] Nocardia e Rhodococcus são importantes componentes da microflora do solo e estão envolvidos na degradação de matéria orgânica e são capazes de decompor compostos químicos ambientalmente prejudiciais (Groth et al., 1999).
[013] Dentre os mecanismos de ação, o antagonismo e antibiose costumam ser o modo de ação predominante de bactérias contra os fitonematóides e fungos. Além do efeito direto na mortalidade dos fitonematóides, esses compostos nematicidas podem apresentar ação direta na eclosão de ovos ou na mobilidade e, efeitos indiretos, como alteração dos exudados radiculares, confusão na locomoção ou indução de resistência também são observados (Sikora & Hoffmann-Hergarten, 1992; Hasky- Gíinther et al. 1998). O uso de bactérias como agentes de controle biológico tem se mostrado uma alternativa promissora no sistema produtivo (Hallmann et al. 2004), como, por exemplo para Meloidogyne graminicola, onde Bacillus megaterium reduziu em 40% a penetração e formação de galhas desse fitonematóide nas raízes de arroz, além de diminuir em 60% sua migração para rizosfera e reduzir em 60% a eclosão de ovos (Sikora & Padgham 2007).
[014] Segundo Da Silva et al. (2007) o uso de Bacillus subtilis para o controle de Pratylenchus brachyurus foi tão bom quanto o tratamento químico utilizando abamectina, apresentando populações extremamente baixas destes fitonematóides no solo, cerca de 1 fitonematoide/cm3 de solo, reduzindo em tomo de 90% esta população frente ao tratamento testemunha. Outros resultados nesta linha de pesquisa, utilizando também Bacillus subtilis para tratamento de sementes é evidenciado por Da Silva et al. (2007), mostrando que esta alternativa de manejo proporcionou reduções na ordem de 50,06% da população de Pratylenchus spp. 30 dias após a semeadura, agregando 18% em produtividade. Resultados semelhantes foram encontrados por Higaki (2012) utilizando Bacillus subtilis para controle de Rotylenchulus reniformis e Pratylenchus brachyurus em algodoeiro. O tratamento com o microrganismo apresentou reduções superiores a 50% na população desses fitonematóides nas raízes da cultura.
[015] Outro dado interessante relatado por Higaki (2012), foi que as plantas tratadas com tal microrganismos apresentaram incrementos de massa fresca de raízes e na parte aérea na ordem de 36 e 47%, respectivamente, frente ao tratamento testemunha. De acordo com Araújo et al. (2008), os mecanismos de ação responsáveis pela promoção de crescimento em plantas podem estar ligados inicialmente à inibição direta do patógeno e pela indução de resistência sistémica, dentre outras. Muitas vezes é difícil reconhecer os mecanismos e relacioná-los à promoção direta de crescimento, visto que mais de um mecanismo é produzido pela bactéria.
[016] Para o controle biológico de fitopatógenos em plantas agricultáveis, Cook & Baker (1983) relatam que a caracterização de efetividade desta prática de manejo é“a redução da soma de inoculo ou das atividades determinantes da doença, provocada por um patógeno, realizada por ou através de um ou mais organismos que não o homem”. Tendo em vista esse conceito de ação, Bettiol et al. (2008) relatam que o uso de Bacillus subtilis tem obtido sucesso para controlar o oídio (causado pelo fungo Uncinula necato ) e a podridão-cinzenta (causado pelo fungo Botrytis cinerea ) da uva e em outras culturas há vários anos no Chile. Segundo Mattos (2010), o uso da mistura de B. subitilis e B. licheniformis, proporcionou reduções significativas da incidência do bolor verde, causado pelo fungo Penicillium digitatum , em frutos de pós colheita da laranja“pêra”
[017] O genêro Bacillus spp. é reconhecidamente um excelente produtor de compostos antimicrobianos (Huang & Chang, 1975; Baker et al., 1983; Motomura & Hirooka, 1996) e a constatação da produção de substâncias inibidoras aos fitopatógenos, produzidas por diversos isolados de Bacillus spp., é especialmente importante para o entendimento dos mecanismos de ação desses microrganismos para o antagonismo. As bactérias antagónicas, a exemplo de B. subtilis , de modo geral agem significativamente por antibiose e, ocasionalmente, por parasitismo e competição. Dessa forma, o controle da doença pode ser efetivo devido às substâncias produzidas e, não apenas pela introdução massal dos agentes de biocontrole (Arras & Arru, 1997). Microrganismos que agem por antibiose, geralmente têm amplo espectro de ação, de forma que na inibição dos fungos a produção de substâncias tóxicas é mais efetiva do que qualquer outro mecanismo de ação envolvido.
[018] Com relação ao estresse abiótico, uma alternativa viável e com resultados significativos é o uso de rizobactérias promotoras de crescimento de plantas (RPCP). Diversos mecanismos associados às RPCPs, que auxiliam as plantas a suportarem os efeitos do estresse hídrico foram descritos, sendo eles: solubilização e fixação de nutrientes, produção de sideróforos, produção de hormônios vegetais, produção de compostos voláteis, alteração na morfologia e arquitetura das raízes, atividade da ACC deaminase, acumulação de osmólitos, ação sobre o sistema antioxidante da planta e produção de exopolissacarídeos (EPS) (ALAMI et al., 2000; ARORA et al., 2001; HUSSAIN et al., 2009; VURUKONDA et al., 2016).
[019] Dentre os mecanismos descritos, a produção de EPS por bactérias tem sido amplamente estudada, porém, visando a aplicação industrial, em especial, nas indústrias alimentícias e farmacêuticas, devido suas características emulsificantes e espessantes (KUMAR et al., 2007). Contudo, dada essas características, a utilização de bactérias produtoras de EPS na agricultura tem despertado interesse, uma vez que podem atuar como importante ferramenta na mitigação dos estresses abióticos.
[020] Apesar dessas vantagens, a maioria dos produtos existentes baseiam-se na premissa de explorar um único microrganismo para o controle biológico de fitopatógenos e fitonematóides, inoculantes, condicionadores de solo, bioestimulantes, redutores de estresse hídrico nas plantas cultivadas e biorremediação. Conforme relatado, alguns agentes possuem mais de um mecanismo de ação, os quais podem atuar isoladamente ou simultaneamente, direta ou indiretamente nos fitonematoides e fitopatógenos, no estímulo ao crescimento de plantas, no condicionamento dos solos, na redução do estresse hídrico em plantas cultivadas e biorremediação. Entretanto, associações entre os diversos agentes ainda é pouco explorado e ampliam o espectro de ação desses microrganismos, favorecendo o crescimento e proteção vegetal e consequentemente o aumento de produtividade com proteção ambiental.
OBJETIVOS DA INVENÇÃO
[021] Assim, o objetivo da presente invenção é proporcionar composições efetivas para a redução de danos causados por fitopatógenos, fitonematoides e pragas agrícolas, promover o crescimento vegetal, condicionar solos e mitigar danos causados por eventuais estresses hídricos ou contaminação ambiental na pulverização, pulverização de sulco de plantio, drench, no recobrimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes em adição à polímeros/excipientes para aplicação nas plantas cultivadas, não se limitando a Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. ( Panicum maximum), Sorghum spp., Pennisetum americanum, Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.), Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis), crotalárias. ( Crotalaria juncea, Crotalaria spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora), dentre outras.
DESCRIÇÃO RESUMIDA DA INVENÇÃO
[022] A presente invenção refere-se a composições biológicas de funções múltiplas, para pulverização, pulverização em sulco, drench, recobrimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas, contendo misturas de pelo menos 2 (dois) gêneros e/ou espécies de Actinomicetos ou seus mutantes, Actinomicetos em misturas com Bacillus ou seus mutantes, Actinomicetos em misturas com fungos ou seus mutantes e combinação entre eles ou parte deles, concomitantemente com defensivos agrícolas (ou não), aditivos e excipientes, em composições biológicas com propriedades fungicidas, nematicidas, inseticidas estimulantes de crescimento, condicionadoras de solos e redução dos efeitos de estresse hídrico em plantas cultivadas e biorremediação e áreas cultivadas.
DESCRIÇÃO DETALHADA DA INVENÇÃO
[023] As composições biológicas de funções múltiplas, compreendem: 1,0 a 20,0% de pelo menos 2 (dois) gêneros e/ou espécies de: Actinomicetos e seus mutantes; Actinomicetos em misturas com representantes dos gêneros de Bacillus; Actinomicetos em misturas com fungos das Ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.);
1,0 a 20,0% de Aditivos;
1,0 a 97,0% de Excipientes; e
5,0 a 10,0% de defensivos agrícolas.
[024] Os Actinomicetos e seus mutantes são do grupo que consiste em Streptomyces spp., Artrobacter spp., Brevibacterium spp., Clavibacter spp., Corynebacterium spp., Nocardia spp, Rhodococcus spp., Micromonospora spp, Nocardioides spp. e Frankia spp..
[025] Os Actinomicetos em misturas com representantes dos gêneros de Bacillus são do grupo que consiste nas espécies de Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus kustaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nigrificans, Bacillus popilliae, pumilus, Bacillus siamensis, Bacillus subtilis, Bacilus licheniformis, Bacillus amyloliquefasciens, Bacillus megaterium, Bacillus sphaericus, Bacillus thuringiensis.
[026] Os Actinomicetos em misturas com fungos das Ordens Hypocreales são do grupo que consiste nas espécies de Trichoderma amazonicum, Trichoderma asperellum, Trichoderma atroviride, Trichoderma aureoviride, Trichoderma austrokoningii, Trichoderma brevicompactum, Trichoderma cinnamomeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma polysporum, Trichoderma pseudokoningii, Trichoderma reesei, Trichoderma stromaticum, Trichoderma virens, Trichoderma viride, Trichoderma viridescens, Clonostachys agarwalii, Clonostachys asymmetrica, Clonostachys aurantia, Clonostachys chlorina, Clonostachys compacta, Clonostachys cylindrospora, Clonostachys farinosa, Clonostachys pseudobotrytis, Clonostachys pulvinata, Clonostachys rosea, (Link) Schroers, Clonostachys spectabilis, Clonostachys theobromae, Purpuriocillium lilacinus, Pochonia bulbillosa, Pochonia chlamydosporia, Pochonia chlamydosporia var. catenulata, Pochonia globispora, Pochonia goniodes, Pochonia humicola, Pochonia microbactrospora, Pochonia parasítica, Pochonia rubescens, Pochonia suchlasporia.
[027] Os Actinomicetos em misturas com Orbiliales são do grupo que consiste nas espécies de Arthrobotrys oligospora, Arthrobotrys aggregata, Arthrobotrys alaskana, Arthrobotrys amerospora, Arthrobotrys botryospora, Arthrobotrys brochopaga, Arthrobotrys conoides, Arthrobotrys constringens, Arthrobotrys cylindrospora, Arthrobotrys dactyloides, Arthrobotrys elegans Arthrobotrys ellipsospora, Arthrobotrys entomopaga, Arthrobotrys globospora, Arthrobotrys irregulares, Arthrobotrys javanica, Arthrobotrys longispora, Arthrobotrys musiformis, Arthrobotrys nematopaga, Arthrobotrys oligospora.
[028] Os Actinomicetos em misturas com Helotiales são do grupo que consiste na espécie de Monacrosporium robustum, Monacrosporium appendiculatum, Monacrosporium ellipsosporum Monacrosporium eudermatum, Monacrosporium fusiforme, Monacrosporium globosporum, Monacrosporium leptosporum.
[029] Os Actinomicetos em misturas com Mucorales são do grupo que consiste na espécie de Cunninghamella elegans.
[030] Os aditivos são dispersantes selecionados do grupo consistindo de polímeros iônicos solúveis em água, polímeros aniônicos solúveis em água, surfactantes selecionados do grupo consistindo de surfactantes aniônicos e surfactantes não iônicos e combinações entre eles.
[031] Os excipientes são do grupo que consiste em: sílicas, talco, bentonita, carboidratos, carbonatos, caseína, soro de leite e derivados de leite e combinações entre eles.
[032] Os defensivos agrícolas consistem em: a) Inseticidas - capsaicin, cartap, celastrus-extract, chlorantraniliprole, chlorbenzuron, chlorethoxyfos, chlorfluazuron, chlorpyrifos-e, cnidiadin, cryolite, cyanophos, cyantraniliprole, cyclaniliprole, cyhalothrin, cyhexatin, cypermethrin, dacnusa, DCIP, dichloropropene, dicofol, diglyphus, diglyphus+dacnusa, dimethacarb, dithioether, dodecylacetate, emamectin, encarsia, EPN, eretmocerus, ethylene-dibromide, eucalyptol, fatty-acids, fatty-acids/salts, fenazaquin, fenobucarb (BPMC), fen pyroxi mate, flubrocythrinate, flufenzine, formetanate, formothion, furathiocarb, gamma-cyhalothrin, garlic- juice, granulosis-virus, harmonia, heliothis armigera NPV, inactive bacterium, indol-3-ylbutyric acid, iodomethane, iron, isocarbofos, isofenphos, isofenphos-m, isoprocarb, isothioate, kaolin, lindane, liuyangmycin, matrine, mephosfolan, metaldehyde, metarhizium-anisopliae, methamidophos, metolcarb (MTMC), mineral-oil, mirex, m-isothiocyanate, monosultap, myrothecium verrucaria, naled, neochrysocharis formosa, nicotine, nicotinoids, oil, oleic-acid, omethoate, orius, oxymatrine, paecilomyces, paraffin-oil, parathion-e, pasteuria, petroleum-oil, pheromones, phosphoras- acid, photorhabdus, phoxim, phytoseiulus, pirimiphos-e, plant-oil, plutella xylostella GV, polyhedrosisvirus, polyphenol-extracts, potassium-oleate, profenofos, prosuler, prothiofos, pyraclofos, pyrethrins, pyridaphenthion, pyrimidifen, pyriproxifen, quillay-extract, quinomethionate, rape-oil, rotenone, saponin, saponozit, sodium-compounds, sodium-fluosilicate, starch, steinemema, streptomyces, sulfluramid, sulphur, tebupirimfos, tefluthrin, temephos, tetradifon, thiofanox, thiometon, triazamate, trichoderma, trichogramma, triflumuron, vertrine, kappa-bifenthrin, kappa-tefluthrin, dichoromezotiaz, broflanilide, pyraziflumid aldicarb, alanycarb, benfuracarb, carbaryl, carbofuran, carbosulfan, methiocarb, methomyl, oxamyl, pirimicarb, propoxur and thiodicarb, acephate, azinphos-ethyl, azinphos-methyl, chlorfenvinphos, chlorpyrifos, chlorpyrifos-methyl, demeton-Smethyl, diazinon, dichlorvos/DDVP, dicrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isoxathion, malathion, methamidaphos, methidathion, mevinphos, monocrotophos, oxymethoate, oxydemeton-methyl, parathion, parathion-methyl, phenthoate, phorate, phosalone, phosmet, phosphamidon, pirimiphos-methyl, quinalphos, terbufos, tetrachlorvinphos, triazophos, trichlorfon, endosulfan, ethiprole, fipronil, pyrafluprole, pyriprole, acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, thiamethoxam, spinosad, spinetoram, mectins, abamectin, emamectin benzoate, ivermectin, lepimectin, milbemectin, hydroprene, kinoprene, methoprene, fenoxycarb, pyriproxyfen; pymetrozine, flonicamid, pyrifluquinazon, clofentezine, hexythiazox, etoxazole, diafenthiuron, fenbutatin oxide, propargite, chlorfenapyr, bensultap, cartap hydrochloride, thiocydam, thiosultap sodium, bistrifluron, diflubenzuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, teflubenzuron, buprofezin, cyromazine, methoxyfenozide, tebufenozide, halofenozide, chromafenozide, amitraz, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim, cyenopyrafen, cyflumetofen, hydramethylnon, acequinocyl, fluacrypyrim, indoxacarb, metaflumizone, spirodiclofen, spiromesifen, spirotetramat, flubendiamide, chlorantraniliprole, cyclaniliprole, cyantraniliprole, azadirachtin, amidoflumet, bifenazate, fluensulfone, piperonyl butoxide, pyridalyl, sulfoxaflor, acrinathrin, allethrin, bifenthrin, cyfluthrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, flucythrinate, tau-fluvalinate, permethrin, silafluofen and tralomethrin, dentre outros b) Fungicidas: benzovindiflupyr, antiperonosporic, ametoctradin, amisulbrom, copper hydroxide, copper oxychloride, copper sulfate, copper persulfate, boscalid, thiflumazide, flutianil, furalaxyl, thiabendazole, benodanil, mepronil, isofetamid, fenfuram, bixafen, fluxapyroxad, penflufen, sedaxane, coumoxystrobin, enoxastrobin, flufenoxystrobin, pyraoxystrobin, pyrametostrobin, triclopyricarb, fenaminstrobin, metominostrobin, pyribencarb, meptyldinocap, fentin acetate, fentin chloride, fentin hydroxide, oxytetracycline, chlozolinate, chloroneb, tecnazene, etridiazole, iodocarb, prothiocarb, extract from Melaleuca alternifolia, pyrisoxazole, oxpoconazole, etaconazole, fenpyrazamine, fenpicoxamide, mefentrifluconazole, naftifine, terbinafine, validamycin, pyrimorph, valifenalate, fthalide, probenazole, isotianil, laminarin, teclofthalam, triazoxide, pyriofenone, chlorothalonil, fluoroimide, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, enilconazole, epoxiconazole, fluquinconazole, fenbuconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, simeconazole, triadimefon, triadimenol, tebuconazole, tetraconazole, triticonazole, prochloraz, pefurazoate, imazalil, triflumizole, cyazofamid, benomyl, carbendazim, thiabendazole, fuberidazole, ethaboxam, etridiazole and hymexazole, azaconazole, diniconazole-M, oxpoconazol, paclobutrazol, uniconazol, l-(4-chloro-phenyl)-2-([l,2,4]triazol-lyl)-cycloheptanol, imazalilsulfphate, azoxystrobin, dimoxystrobin, enestroburin, fluoxastrobin, kresoxim-methyl, methominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin, enestroburin, methyl (2-chloro-5-[l-(3- methylbenzyloxyimino)ethyl]benzyl)carbamate, methyl (2-chloro-5-[l-(6- methylpyridin-2- ylmethoxyimino)ethyl]benzyl)carbamate, methyl 2-(ortho- (2,5-dimethylphenyloxymethylene)- phenyl)-3-methoxyacrylate, 2-(2-(6-(3- chloro-2-methyl-phenoxy)-5-fluoro-pyrimidin-4-yloxy)- phenyl)-2- methoxyimino-N-methyl-acetamide, 3-methoxy-2-(2-(N-(4-methoxy-phenyl)- cyclopropanecarboximidoylsulfanylmethyl)-phenyl)-acrylic acid methyl ester; carboxin, benalaxyl, benalaxyl-M, fenhexamid, flutolanil, furametpyr, mepronil, metalaxyl, mefenoxam, ofurace, oxadixyl, oxycarboxin, penthiopyrad, isopyrazam, thifluzamide, tiadinil, 3,4-dichloro-N-(2- cyanophenyl)isothiazole-5-carboxamide, dimethomorph, flumorph, flumetover, fluopicolide (picobenzamid), zoxamide, carpropamid, diclocymet, mandipropamid, N- (2-(4-[3-(4-chlorophenyl)prop-2-ynyloxy]-3- methoxyphenyl)ethyl)-2-methanesulfonyl-amino-3- methylbutyramide, N-(2- (4-[3-(4-chlorophenyl)prop-2-ynyloxy]-3-methoxy-phenyl)ethyl)-2- ethanesulfonylamino- 3 -methylbutyramide, methyl 3-(4-chlorophenyl)-3-(2- isopropoxycarbonylamino-3-methyl-butyrylamino)propionate, N-(4'- bromobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N- (4'-trifluoromethyl-biphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5- carboxamide, N-(4'- chloro-3'-fluorobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N-(3',4'-dichloro-4-fluorobiphenyl-2-yl)-3- difluoro-methyl-l-methylpyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-3-difluoromethyl-lmethylpyrazole-4-carboxamide, N-(2- cyano-phenyl)- 3,4-dichloroisothiazole-5-carboxamide, 2- amino-4-methyl- thiazole-5-carboxanilide, 2-chloro-N-(l,l,3-trimethyl-indan-4-yl)- nicotinamide, N- (2-(l,3-dimethylbutyl)-phenyl)-l,3-dimethyl-5-fluoro-lH- pyrazole-4-carboxamide, N-(4'-chloro3',5-difluoro-biphenyl-2-yl)-3- difluoromethyl-l-methyl-IH-pyrazole-4-carboxamide, N-(4'-chloro3',5- difluoro-biphenyl- 2-yl)-3-trifluoromethyl-l -methyl-lH-pyrazole-4- carboxamide, N-(3',4'- dichloro-5-fluoro-biphenyl-2-yl)-3-trifluoromethyl-l- methyl-lH-pyrazole-4- carboxamide, N-(3',5difluoro-4'-methyl-biphenyl-2- yl)-3-difluoromethyl- 1 -methyl-lH-pyrazole-4-carboxamide, N- (3',5- difluoro-4'-methyl-biphenyl-2-yl)-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N- (cis-2-bicyclopropyl-2-yl-phenyl)-3-difluoromethyl-l methyl-lH-pyrazole-4-carboxamide, N- (trans-2-bicyclopropyl-2-yl-phenyl)- 3-difluoro-methyl-l-methyl-l H-pyrazole-4-carboxamide, fluopyram, N-(3- ethyl-3,5-5- trimethyl-cyclohexyl)-3-formylamino-2-hydroxy-benzamide, oxytetracyclin, silthiofam, N-(6-methoxy-pyridin-3-yl) cyclopropanecarboxamide, 2-iodo-N-phenylbenzamide, N-(2-bicyclo-propyl- 2-yl-phenyl)-3-difluormethyl-l-methylpyrazol-4-ylcarboxamide, N- (3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethyl-5-fluoropyrazol-4-yl-carboxamide, N- (3',4',5'-trifluorobiphenyl-2-yl)-5-chlorol,3-dimethyl- pyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-fluoromethyl- lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-lmethylpyrazol-4-ylcarboxamide,N-(3',4',5'- trifluorobiphenyl-2-yl)-3 -difluoromethyl-lmethylpyrazol-4-ylcarboxamide, N - (3',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-lmethylpyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-chloro-3-difluoromethyl-l - methylpyrazol-4-ylcarboxamide, N- (3', 4', 5'-trifluorobiphenyl-2-yl)-3- (chlorodifluoromethyl)-lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-fluoro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5- chloro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)- 1 ,3-dimethyl-5-fluoropyrazol-4-ylcarboxamide, N- (2, ,4 , ,5'- trifluorobiphenyl-2-yl)-5-chloro-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-fluoromethyl-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-l-methylpyrazol-4-ylcarboxamide,N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2- yl)-5-chloro- 3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N- (2',4',5'- trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)-l-methylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5 trifluorobiphenyl-2-yl)- 5-fluoro-l-methyl-3-trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-5-chloro-l-methyl-3-trifluoromethylpyrazol-4- ylcarboxamide, N-(3',4'- dichloro-3-fluorobiphenyl-2-yl)-l -methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'- dichloro-3- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-l H-pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl- S-difluoromethyl-1 H-pyrazole-4-carboxamide, N-(3'- chloro-4'-fluoro-3- fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3A4'-dichloro-4-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-difluoro-4-fluorobiphenyl-2-yl)-l - methyl- S-trifluoromethyl-lH-pyrazole-4-carboxamide, N- (3 ',4'-dichloro-4- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4- carboxamide, N- (3M'-difluoro-4-fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH- pyrazole-4-carboxamide, N- (3'-chloro-4'-fluoro-4-fluorobiphenyl-2-yl)-l- methyl-5-difluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide, N-(3',4'-difluoro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-l - methyl- S-difluoromethyl-IH-pyrazole-carboxamide, N- (3',4'-difluoro-5- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3',4'-dichloro-5-fluorobiphenyl-2-yl)-l,3-dimethyl-l H-pyrazole-4- carboxamide, N-(3'-chloro-4'- fluoro-5-fluorobiphenyl-2-yl)-l-methyl-3- difluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-4-fluorobiphenyl-2- yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'-fluoro- 5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide,N-(4'- chloro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl- lH-pyrazole-4-carboxamide, N-(4'- methyl-5-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-5-fluorobiphenyl- 2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-chloro-5- fluorobiphenyl-2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-methyl- 5-fluorobiphenyl-2- yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4- carboxamide, N-(4'-chloro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-[2-(l, 1,2, 3,3,3- hexafluoropropoxy)-phenyl]-3- difluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N-[4'-(trifluoromethylthio)-biphenyl-2-yl]- 3-difluoromethyl-l- methyl-lH-pyrazole-4-carboxamide and N-[4'-(trifluoromethylthio)- biphenyl2-yl]-l-methyl-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, fluazinam, pyrifenox, bupirimate, cyprodinil, fenarimol, ferimzone, mepanipyrim, nuarimol, pyrimethanil, triforine, fenpiclonil, fludioxonil, aldimorph, dodemorph, fenpropimorph, tridemorph, fenpropidin, iprodione, procymidone, vinclozolin, famoxadone, fenamidone, octhilinone, probenazole, 5-chloro-7-(4-methyl-piperidin-l-yl)-6-(2,4,6- trifluorophenyl)- [l,2,4]triazolo[l,5-a]pyrimidine, anilazine, diclomezine, pyroquilon, proquinazid, tricyclazole, 2-butoxy-6-iodo-3-propylchromen-4-one, acibenzolar-S-methyl, captafol, captan, dazomet, folpet, fenoxanil, quinoxyfen, N,N-dimethyl-3-(3-bromo-6-fluoro-2-methylindole-lsulfonyl)- [l,2,4]triazole-l-sulfonamide, 5-ethyl-6-octyl-[l,2,4]triazolo[l,5- a]pyrimidin- 2,7-diamine, 2,3,5,6-tetrachloro-4-methanesulfonyl-pyridine, 3,4,5-trichloro- pyridine-2,6-di-carbonitrile, N-(l- (5-bromo-3-chloro-pyridin-2-yl)-ethyl)-2,4- dichloro-nicotinamide, N-((5-bromo-3-chloropyridin-2- yl)-methyl)-2,4- dichloro-nicotinamide, diflumetorim, nitrapyrin, dodemorphacetate, fluoroimid, blasticidin-S, chinomethionat, debacarb, difenzoquat, difenzoquat-methylsulphat, oxolinic acid, piperalin, mancozeb, maneb, metam, methasulphocarb, medram, ferbam, propineb, thiram, zineb, ziram, diethofencarb, iprovalicarb, benthiavalicarb, propamocarb, propamocarb hydrochlorid, 4-fluorophenyl N-(l-(l-(4-cyanophenyl)-ethanesulfonyl)but-2- yl)carbamate, methyl 3-(4-chloro-phenyl)-3-(2- isopropoxycarbonylamino-3- methylbutyrylamino)propanoate, guanidine, iminoctadine, guazatine, kasugamycin, oxytetracyclin, streptomycin, polyoxin, validamycin, binapacryl, dinocap, dinobuton, sulfur-containing heterocyclyl compounds: dithianon, isoprothiolane, edifenphos, iprobenfos, fosetyl, fosetyl-aluminum, pyrazophos, tolclofos- methyl, dichlofluanid, flusulfamide, hexachloro- benzene, phthalide, pencycuron, quintozene, thiophanate, thiophanatemethyl, cyflufenamid, cymoxanil, dimethirimol, ethirimol, furalaxyl, metrafenone and spiroxamine, guazatine-acetate, iminoc- tadine-triacetate, iminoctadinetris(albesilate), kasugamycin hydrochloride hydrate, dichlorophen, pentachlorophenol and its salts, N-(4- chloro-2-nitro-phenyl)- N-ethyl-4-methyl-benzenesulfonamide, dicloran, nitrothal-isopropyl, tecnazen, biphenyl, bronopol, diphenylamine, mildiomycin, oxincopper, prohexadione calcium, N - (cyclopropylmethoxyimino- (6-difluoromethoxy- 2,3-difluoro-phenyl)-methyl)-2-ph acetamide, N'-(4-(4-chloro-3- trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl
formamidine, N'-(4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl- phenyl)-N-ethyl-N-methyl formamidine, N'-(2-methyl-5-trifluormethyl-4-(3- trimethylsilanyl-propoxy)-phenyl)-N-ethyl-Nmethylformamidine and N'-(5- difluormethyl-2-methyl- 4-(3-trimethylsilanyl-propoxy)-phenyl)-Nethyl-N- methyl formamidine. c) Herbicides: alloxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tralkoxydim, butroxydim, clefoxydim, tepraloxydim; phenoxyphenoxypropionic esters, clodinafop-propargyl, cyhalofop-butyl, diclofop-methyl, fenoxa prop-ethyl, fenoxaprop-P-ethyl, fenthiapropethyl, fluazifop-butyl, fluazifop-P-butyl, haloxyfop-ethoxyethyl, haloxyfop-methyl, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop-ethyl, quizalofop-P-ethyl o r quizalofop-tefuryl, flamprop-methyl, flamprop-isopropyl, imazapyr, imazaquin, imazamethabenz-methyl (imazame), imazamox, imazapic, imazethapyr, pyrithiobac-acid, pyrithiobac- sodium, bispyribac-sodium, pyribenzoxym, florasulam, flumetsulam, metosulam, amidosulfuron, azimsulfuron, bensulfuron-methyl, chlorimuron- ethyl, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron-methyl, ethoxysulfuron, flazasulfuron, halosulfuron-methyl, imazosulfuron, metsulfuron-methyl, nicosulfuron, primisulfuron-methyl, prosulfuron, pyrazosulfuron-ethyl, rimsulfuron, sulfometuron-methyl, thifensulfuron- methyl, triasulfuron, tribenuron-methyl, triflusulfuron-methyl, tritosulfuron, sulfosulfuron, foramsulfuron, iodosulfuron, benzoyl prop-ethyl, bromobutide, chiorthiamid, diphenamid, etobenzanidibenzchlomet, fluthiamide, fosamin, monalide, clopyralid, picloram, 2,4-D, benazolin, naptalame, diflufenzopyr, benzofenap, clomazone, diflufenican, fluorochloridone, fluridone, pyrazolynate, pyrazoxyfen, isoxaflutole, isoxachlortole, mesotrione, sulcotrione, ketospiradox, flurtamone, norflurazon, amitrol, glyphosate, sulfosate, bilanafos, glufosinateammonium, anilofos, mefenacet, dimethenamid, S-dimethenamid, acetochlor, alachlor, butachlor, butenachlor, diethatyl-ethyl, dimethachlor, metazachlor, metolachlor, S-metolachlor, pretilachlor, propachlor, prynachlor, terbuchlor, thenylchlor, xylachlor, butylate, cycloate, di-allate, dimepiperate, EPTC. esprocarb, molinate, pebulate, prosulfocarb, thiobencarb, tri-allate, vemolate; benfuresate, perfluidone; asulam, carbetamid, chlorpropham, orbencarb, pronamid, propham, tiocarbazil benefin, butralin, dinitramin, ethalfluralin, fluchloralin, oryzalin, pendimethalin, prodiamine, trifluralin dithiopyr, thiazopyr, butamifos, chlorthal-dimethyl (DCPA), acifluorfen, acifluorfen-sodium, aclonifen, bifenox, chlomitrofen (CNP), ethoxyfen, fluorodifen, fluoroglycof enethyl, fomesafen, furyloxyfen, lactofen, nitrofen, nitrofluorfen or oxyfluorfen, oxadiargyl, oxadiazon, azafenidin, butafenacil, carfentrazone- ethyl, cinidon-ethyl, flumiclorac-pentyl, flumioxazin, flumipropyn, flupropacil, fluthiacet-methyl, sulfentrazone, thidiazimin, nipyraclofen, propanil, pyridate or pyridafol, bromofenoxim, dinoseb, dinoseb-acetate, dinoterb, cyperquat-chloride, difenzoquat-methylsulfate, diquat or paraquatdichloride, chlorbromuron, chlorotoluron, difenoxuron, dimefuron, diuron, ethidimuron, fenuron, fluometuron, isoproturon, isouron, linuron, methabenzthiazuron, methazole, metobenzuron, metoxuron, monolinuron, neburon, siduron, tebuthiuron, bromoxynil, ioxynil, chloridazon, ametryn, atrazine, cyanazine, desmein, dimethamethryn, hexazinone, prometon, prometryn, propazine, simazine, simetryn, terbumeton, terbutryn, terbutylazine, trietazine, metamitron, metribuzin bromacil, lenacil, terbacil, desmedipham, phenmedipham, tridiphane, isoxaben, dichlobenil, dalapon, ethofumesate, chlorfenac, aziprotryn, barban, bensulide, benzthiazuron, benzofluor, buminafos, buthidazole, buturon, cafenstrole, chlorbufam, chlorfenprop-methyl, chloroxuron, cinmethylin, cumyluron, cycluron, cyprazine, cyprazole, dibenzyluron, dipropetryn, dymron, eglinazin-ethyl, endothall, ethiozin, flucabazone, fluorbentranil, flupoxam, isocarbamid, isopropalin, karbutilate, mefluidide, monuron, napropamide, napropanilide, nitralin, oxaciclomefone, phenisopham, piperophos, procyazine, profluralin, pyributicarb, secbumeton, sulfallate (CDEC), terbucarb, triaziflam, triazofenamid, trimeturon, dentre outros d) Nematicidas: Benomyl, cloethocarb, aldoxycarb, tirpate, diamidafos, fenamiphos, cadusafos, dichlofenthion, ethoprophos, fensulfothion, fosthiazate, heterophos, isamidofof, isazofos, phosphocarb, thionazin, imicyafos, mecarphon, acetoprole, benclothiaz, chloropicrin, dazomet, fluensulfone, 1,3- dichloropropene (telone), dimethyl disulfide, metam sodium, metam potassium, metam salt (all MITC generators), methyl bromide, steam fumigation of soil, allyl isothiocyanate (AITC), dimethyl sulfate, furfual (aldehyde), dentre outros.
[033] As composições devem ser empregadas como formulação em pó molhável. Entretanto, outras formulações contendo esses microrganismos, tais como, emulsões, microemulsões, suspensões, suspensões concentradas, grânulos, encapsulamento, microencapsulamento, nanoencapsulamento, dentre outras, também podem ser utilizadas.
[034] As composições biológicas de funções múltiplas são para o controle biológico fitopatógenos, fitonematóides e pragas agrícolas, inoculante, solubilização de fósforo, condicionamento de solos, bioestimulante, redução de estresse hídrico em plantas cultivadas e biorremediação em áreas agricultáveis ou não com efeitos nematicidas na redução dos danos e controle de fitonematóides, não se limitando à Pratylenchus brachyurus, Pratylenchus zea, Meloidogyne incógnita, Meloidogyne javanica, Meloidogyne exigua, Heterodera glycines, Rotylenchulus reniformis, Pratylenchus coffea, Helicotilechus dihystera, com efeitos fungicidas na redução dos danos e controle, não se limitando à Phakopsora pachyrhizi, Corynespora cassucola, Puccinia polysora, Exserohilum turcicum, Xcmthomonas axonopodis, Ramuíaria areola, Hemileia vastatrix, Cercospora coffeicola, Phoma spp., Pseudomonas syringae, Rhizoctonia solani, Fusarium solani, Fusarium oxysporum, Fusarium graminearum, Fusarium semitectum, Colletotrichum gloeosporioides, Colletotrichum truncatum, Colletotrichum lindemuthianum, Macrophomna phaseolina, Sclerotinia sclerotiorum, Botrytis cinerea, Penicillium digitatum, com ação inseticida na redução dos danos e controle, não se limitando a pragas Polyphagotarsonemus latus, Tetranychus urticae, Diabrotica speciosa, Anticarsia gemmatalis, Spodoptera cosmioides, Spodoptera frugiperda, Pseudoplusia includens, Trichoplusia ni, Bemisia tabaci, Euschistos heros, Nezara viridula, Leucoptera cojfeella, Hypothenemus hampei, Oligonychus ilicis, Polyphagotarsonemus latus , Brevipalpus phoenicis, Planococcus citri, Planococcus minor, com ação de promoção de crescimento, condicionador de solos, bioestimulantes, na mitigação do estresse hídrico das plantas cultivadas e biorremediação em áreas agricultáveis ou não, em adição aos polímeros/excipientes em formulações para pulverização, pulverização de sulco de plantio, drench, revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas, não se limitando a Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta ), Brachiaria spp. ( Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp , Nicotiana tabacum, ornamentais, frutíferas 0 Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis ), crotalárias. {Crotalaria juncea, Crotaldria spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora ), dentre outras.
EXEMPLO DE OBTENÇÃO DE GÊNEROS E OU ESPÉCIES DE ACTINOMICETOS E SEUS MUTANTES
[035] A título de exemplo, na obtenção de pelo menos 2 (dois) gêneros e/ou espécies de: Actinomicetos e seus mutantes; Actinomicetos em misturas com representantes dos gêneros de Bacillus, Actinomicetos em misturas com fungos das Ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.) temos:
Exemplo 1:
• Streptomyces flavovirences - 1,0 x 106 u.f.c./g
• Nocardia erythropolis 1,0 x 106 u.f.c./g
Exemplo 2:
• Streptomyces grisus- 1,0 x 106 u.f.c./g
• Bacillus licheniformis 1,0 x 1010 u.f.c./g
• Bacillus amyloliquenfacies 1,0 x 1010 u.f.c./g
Exemplo 3:
• Nocardia asteroides- 1,0 x 106 u.f.c./g
• Bacillus licheniformis 1,0 x 1010 u.f.c./g
• Trichoderma harzianum 1,0 x 10 u.f.c./g
• Purpuriocillium lilacinus 1,0 x 109 u.f.c./g
Exemplo 4: • Streptomyces purpuracens: 1,0 x 106 u.f.c./g
• Bacillus thuringiensis: 1,0 x 109 u.f.c./g
• Beauveria bassiana: 1,0 x 10 u.f.c./g
[036] A título de exemplo a composição poderá apresentar as seguintes concentrações:
Exemplo 1 :
Streptomyces flavovirences: 1,0 a 20,0%
Nocardia erythropolis : 1,0 a 20,0%
Aditivos: 1,0 a 20,0%
Excipientes: 97,0 a 40,0%
Exemplo 2:
Streptomyces grisus : 1,0 a 20,0%
Bacillus licheniformis : 1,0 a 20,0%
Bacillus amyloliquefaciens : 1,0 a 20,0%
Aditivos: 1,0 a 20,0%
Excipientes: 91,0 a 10,0%
Azoxistrobin 5,0 a 10,0%
Exemplo 3:
Nocardia asteroides : 1,0 a 20,0%
Bacillus licheniformis : 1,0 a 15,0%
Trichoderma harzianum : 1,0 a 20,0%
Purpuriocillium lilacinus: 1,0 a 15,0%
Aditivos: 1,0 a 20,0%
Excipientes: 95,0 a 10,0%
Exemplo 4:
Streptomyces purpuracens : 1,0 a 20,0%
Bacillus thuringiensis : 1,0 a 20,0% Beauveria bassiana: 1,0 a 20,0%
Aditivos: 1,0 a 20,0%
Excipientes: 96,0 a 20,0%
EXEMPLOS DE OBTENÇÃO DA COMPOSIÇÃO
[037] Uma composição contendo 20,0% de Streptomyces flavovirences, 20,0% de Nocardia erythropolis, 3,0 % de polímero de estireno acrílico, 1,0% surfactante aniônico e 56,0% de inerte foi formulada para avaliar sua eficiência no controle de fitonematóides, fitopatógenos e biostimulação.
[038] Uma composição contendo 20,0% de Streptomyces grisus , 7,0% de Bacillus licheniformis, 7% de Bacillus amyloliquefaciens, 3,0% de polímero de estireno acrílico, 1,0% surfactante aniônico, 10,0% de azoxistrobin e 52,0% de inerte foi formulada para avaliar sua eficiência no controle de fitopatógenos, estímulo ao crescimento foliar e radicular e redução ao estresse hídrico.
[039] Uma composição contendo 20,0% de Nocardia asteroides, 7,0 % de Bacillus licheniformis , 20,0% de Trichoderma harzianum, 10,0% de Purpuriocillium lilacinus, 3,0 % de polímero de estireno acrílico, 1,0% surfactante aniônico e 39,0% de inerte foi formulada para avaliar sua eficiência no controle de fitopatógenos, estímulo ao crescimento foliar e radicular e redução ao estresse hídrico.
[040] Uma composição contendo 20,0% de Streptomyces purpuracens, 7,0% de Bacillus thuringiensis, 20,0% de Beauveria bassiana, 3,0% de polímero de estireno acrílico, 1 ,0% surfactante aniônico e 49,0% de inerte foi formulada para avaliar sua eficiência no controle de insetos.
[041] As composições para o controle biológico de fitopatógenos, fitonematóides e insetos, inoculantes, condicionadores de solo, bioestimulantes, redutores de estresse hídrico em plantas e biorremediação deverá seguir o seguinte fluxo de eventos:
• Matéria prima: as matérias primas que irão compor o produto deverão ser recebidas e manipuladas por pessoas treinadas;
• Mistura: as matérias primas deverão ser pesadas e misturadas seguindo o procedimento operacional padrão (POP) nas proporções indicadas;
• Amostragem: após a mistura, deverão ser retiradas amostras para verificação e certificação das garantias do produto. Deve-se analisar número de unidades formadoras de colónias (u.f.c.)/g de produto;
• Envase: o produto formulado que se apresentar dentro das especificações de garantia deverão ser envasados em frascos plásticos de 1,0; 5,0 e 10,0 kg, previamente rotulados. Os frascos/pouchs são selados e fechados;
• Armazenagem: os frascos/pouchs são acomodados em pallets e armazenados em local seco, arejado e protegido da luz, permanecendo nessa condição até ser despachado.
[042] O presente invento poderá ser aplicado tanto por pulverização via barra, pulverização via sulco de plantio, drench, como recobrimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas para o controle biológico de fitopatógenos, fitonematóides e insetos, inoculantes, condicionadores de solo, bioestimulantes e redutores de estresse hídrico para as plantas cultivadas e biorremediação. O método de aplicação deverá ser analisado caso a caso e dependerá das condições técnicas e necessidade de cada produtor.
VANTAGENS OBTIDAS
[043] O uso da composição para o controle biológico de fitopatógenos, fitonematóides e insetos, inoculantes, condicionadores de solo, bioestimulantes e redutores de estresse hídrico para as plantas cultivadas e biorremediação contendo misturas de Actinomicetos ou Actinomicetos em misturas com bactérias e/ou fungos, oferecem as seguintes vantagens:
• favorece desenvolvimento das plantas cultivados, propiciando incrementos nos parâmetros de crescimento radicular, foliar e de produção;
• E uma alternativa ao uso de fungicidas, nematicidas e inseticidas químicos, vindo ao encontro do interesse da sociedade para produtos mais ecologicamente seguros;
• Explora uma gama maior de mecanismos de ação contra fitonematóides, garantindo, assim, uma maior eficiência;
• Explora uma gama maior de mecanismos de ação contra fitopatógenos, garantindo, assim, uma maior eficiência;
• Explora uma gama maior de mecanismos de ação contra insetos, garantindo, assim, uma maior eficiência;
• Explora uma gama maior de mecanismos de ação na fixação de nitrogénio nas plantas, garantindo uma maior eficiência nutricional;
• Explora uma gama maior de mecanismos no condicionamento dos solos, reduzindo os fitopatógenos e aumentando a interação com as raízes e promovendo o crescimento radicular e foliar das plantas cultivadas;
• Explora uma gama maior de mecanismos de ação contra estresses abióticos, principalmente estresse hídrico, garantindo, assim, uma maior eficiência na retenção de água e incremento produtivo;
• Explora diferentes mecanismos de ação na degradação de substâncias danosas ao meio ambiente, a exemplo de pesticidas;
• Diminui a seleção de fitonematóides, fitopatógenos e insetos resistentes a produtos químicos; • Possui facilidade operacional para o emprego da tecnologia na pulverização, pulverização de sulco, drench e recobrimento, tratamento e incrustação de semente de forma industrial ou não;
• E capaz de ser empregado nos constituintes do revestimento, tratamento e incrustação de sementes (industrial ou não) de Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.j, Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis ), crotalárias. ( Crotalaria juncea, Crotalária spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora). no tratamento industrial ou não.
[044] A abrangência da presente patente de invenção, não deve ser limitada aos exemplos de aplicação, mas sim, aos termos definidos nas reivindicações e seus equivalentes.
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Claims

REIVINDICAÇÕES
1 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, caracterizada por compreender:
1,0 a 20,0% de pelo menos 2 (dois) gêneros e/ou espécies de: Actinomicetos e seus mutantes; Actinomicetos em misturas com representantes dos gêneros de Bacillus Actinomicetos em misturas com fungos das Ordens Hypocreales, Orbiliales, Helotiales e Mucorales, e combinação entre eles ou parte deles (em unidades formadoras de colónia, u.f.c.) em conjunto ou não com defensivos agrícolas (fungicidas, inseticidas, acaricidas, herbicidas e nematicidas);
1,0 a 20,0% de Aditivos;
1,0 a 97,0 % de Excipientes;
5,0 a 10,0% de defensivos agrícolas.
2 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos Actinomicetos e seus mutantes serem do grupo que consiste em Streptomyces spp., Artrobacter spp., Brevibacterium spp., Clavibacter spp., Corynebacterium spp., Nocardia spp, Rhodococcus spp., Micromonospora spp, Nocardioides spp. e Frankia spp.
3 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos Actinomicetos em misturas com representantes dos gêneros de Bacillus serem do grupo que consiste nas espécies de Bacillus agri, Bacillus aizawai, Bacillus albolactis, Bacillus firmus, Bacillus coagulans, Bacillus endoparasiticus, Bacillus endorhythmos, Bacillus kustaki, Bacillus lacticola, Bacillus lactimorbus, Bacillus lactis, Bacillus laterosporus, Bacillus lentimorbus, Bacillus megaterium, Bacillus medusa, Bacillus metiens, Bacillus natto, Bacillus nigrificans, Bacillus popilliae, pumilus, Bacillus siamensis, Bacillus subtilis, Bacilus licheniformis, Bacillus amyloliquefasciens, Bacillus megaterium, Bacillus sphaericus, Bacillus thuringiensis
4 COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos Actinomicetos em misturas com fungos das Ordens Hypocreales serem do grupo que consiste nas espécies de Trichoderma amazonicum, Trichoderma asperellum, Trichoderma atroviride, Trichoderma aureoviride, Trichoderma austrokoningii, Trichoderma brevicompactum, Trichoderma cinnamomeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma polysporum, Trichoderma pseudokoningii, Trichoderma reesei, Trichoderma stromaticum, Trichoderma virens, Trichoderma viride, Trichoderma viridescens, Clonostachys agarwalii, Clonostachys asymmetrica, Clonostachys aurantia, Clonostachys chlorina, Clonostachys compacta, Clonostachys cylindrospora, Clonostachys farinosa, Clonostachys pseudobotrytis, Clonostachys pulvinata, Clonostachys rosea, (Link) Schroers, Clonostachys spectabilis, Clonostachys theobromae, Purpuriocillium lilacinus, Pochonia bulbillosa, Pochonia chlamydosporia, Pochonia chlamydosporia var. catenulata, Pochonia globispora, Pochonia goniodes, Pochonia humicola, Pochonia microbactrospora, Pochonia parasítica, Pochonia rubescens, Pochonia suchlasporia.
5 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos Actinomicetos em misturas com Orbiliales serem do grupo que consiste nas espécies de Arthrobotrys oligospora, Arthrobotrys aggregata, Arthrobotrys alaskana, Arthrobotrys amerospora, Arthrobotrys botryospora, Arthrobotrys brochopaga, Arthrobotrys conoides, Arthrobotrys constringens Dowsett, Arthrobotrys cylindrospora, Arthrobotrys dactyloides, Arthrobotrys elegans Arthrobotrys ellipsospora, Arthrobotrys entomopaga, Arthrobotrys globospora, Arthrobotrys irregulares, Arthrobotrys javanica, Arthrobotrys longispora, Arthrobotrys musiformis, Arthrobotrys nematopaga, Arthrobotrys oligospora.
6 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos
Actinomicetos em misturas com Helotiales serem do grupo que consiste na espécie de Monacrosporium robustum, Monacrosporium appendiculatum, Monacrosporium ellipsosporum Monacrosporium eudermatum,
Monacrosporium fusiforme, Monacrosporium globosporum, Monacrosporium leptosporum.
7 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos
Actinomicetos em misturas com Mucorales serem do grupo que consiste na espécie de Cunninghamella elegans.
8 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos defensivos serem compostos pelos seguintes princípios ativos: a) Inseticidas
- capsaicin, cartap, celastrus-extract, chlorantraniliprole, chlorbenzuron, chlorethoxyfos, chlorfluazuron, chlorpyrifos-e, cnidiadin, cryolite, cyanophos, cyantraniliprole, cyclaniliprole, cyhalothrin, cyhexatin, cypermethrin, dacnusa, DCIP, dichloropropene, dicofol, diglyphus, diglyphus+dacnusa, dimethacarb, dithioether, dodecylacetate, emamectin, encarsia, EPN, eretmocerus, ethylene-dibromide, eucalyptol, fatty-acids, fatty-acids/salts, fenazaquin, fenobucarb (BPMC), fen pyroxi mate, flubrocythrinate, flufenzine, formetanate, formothion, furathiocarb, gamma-cyhalothrin, garlic- juice, granulosis-virus, harmonia, heliothis armigera NPV, inactive bacterium, indol-3-ylbutyric acid, iodomethane, iron, isocarbofos, isofenphos, isofenphos-m, isoprocarb, isothioate, kaolin, lindane, liuyangmycin, matrine, mephosfolan, metaldehyde, metarhizium-anisopliae, methamidophos, metolcarb (MTMC), mineral-oil, mirex, m-isothiocyanate, monosultap, myrothecium verrucaria, naled, neochrysocharis formosa, nicotine, nicotinoids, oil, oleic-acid, omethoate, orius, oxymatrine, paecilomyces, paraffin-oil, parathion-e, pasteuria, petroleum-oil, pheromones, phosphoras- acid, photorhabdus, phoxim, phytoseiulus, pirimiphos-e, plant-oil, plutella xylostella GV, polyhedrosisvirus, polyphenol-extracts, potassium-oleate, profenofos, prosuler, prothiofos, pyraclofos, pyrethrins, pyridaphenthion, pyrimidifen, pyriproxifen, quillay-extract, quinomethionate, rape-oil, rotenone, saponin, saponozit, sodium-compounds, sodium-fluosilicate, starch, steinemema, streptomyces, sulfluramid, sulphur, tebupirimfos, tefluthrin, temephos, tetradifon, thiofanox, thiometon, triazamate, trichoderma, trichogramma, triflumuron, vertrine, kappa-bifenthrin, kappa-teflutbrin, dichoromezotiaz, broflanilide, pyraziflumid aldicarb, alanycarb, benfuracarb, carbaryl, carbofuran, carbosulfan, methiocarb, methomyl, oxamyl, pirimicarb, propoxur and thiodicarb, acephate, azinphos-ethyl, azinphos-methyl, chlorfenvinphos, chlorpyrifos, chlorpyrifos-methyl, demeton-Smethyl, diazinon, dichlorvos/DDVP, dicrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isoxathion, malathion, methamidaphos, methidathion, mevinphos, monocrotophos, oxymethoate, oxydemeton-methyl, parathion, parathion-methyl, phenthoate, phorate, phosalone, phosmet, phosphamidon, pirimiphos-methyl, quinalphos, terbufos, tetrachlorvinphos, triazophos, trichlorfon, endosulfan, ethiprole, fipronil, pyrafluprole, pyriprole, acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, thiamethoxam, spinosad, spinetoram, mectins, abamectin, emamectin benzoate, ivermectin, lepimectin, milbemectin, hydroprene, kinoprene, methoprene, fenoxycarb, pyriproxyfen; pymetrozine, flonicamid, pyrifluquinazon, clofentezine, hexythiazox, etoxazole, diafenthiuron, fenbutatin oxide, propargite, chlorfenapyr, bensultap, cartap hydrochloride, thiocydam, thiosultap sodium, bistrifluron, diflubenzuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, teflubenzuron, buprofezin, cyromazine, methoxyfenozide, tebufenozide, halofenozide, chromafenozide, amitraz, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim, cyenopyrafen, cyflumetofen, hydramethylnon, acequinocyl, fluacrypyrim, indoxacarb, metaflumizone, spirodiclofen, spiromesifen, spirotetramat, flubendiamide, chlorantraniliprole, cyclaniliprole, cyantraniliprole, azadirachtin, amidoflumet, bifenazate, fluensulfone, piperonyl butoxide, pyridalyl, sulfoxaflor, acrinathrin, allethrin, bifenthrin, cyfluthrin, lambda-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, flucythrinate, tau-fluvalinate, permethrin, silafluofen and tralomethrin, dentre outros b) Fungicidas: benzovindiflupyr, antiperonosporic, ametoctradin, amisulbrom, copper hydroxide, copper oxychloride, copper sulfate, copper persulfate, boscalid, thiflumazide, flutianil, furalaxyl, thiabendazole, benodanil, mepronil, isofetamid, fenfuram, bixafen, fluxapyroxad, penflufen, sedaxane, coumoxystrobin, enoxastrobin, flufenoxystrobin, pyraoxystrobin, pyrametostrobin, triclopyricarb, fenaminstrobin, metominostrobin, pyribencarb, meptyldinocap, fentin acetate, fentin chloride, fentin hydroxide, oxytetracycline, chlozolinate, chloroneb, tecnazene, etridiazole, iodocarb, prothiocarb, extract from Melaleuca alternifolia, pyrisoxazole, oxpoconazole, etaconazole, fenpyrazamine, fenpicoxamide, mefentrifluconazole, naftifine, terbinafine, validamycin, pyrimorph, valifenalate, fthalide, probenazole, isotianil, laminarin, teclofthalam, triazoxide, pyriofenone, chlorothalonil, fluoroimide, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, enilconazole, epoxiconazole, fluquinconazole, fenbuconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, simeconazole, triadimefon, triadimenol, tebuconazole, tetraconazole, triticonazole, prochloraz, pefurazoate, imazalil, triflumizole, cyazofamid, benomyl, carbendazim, thiabendazole, fuberidazole, ethaboxam, etridiazole and hymexazole, azaconazole, diniconazole-M, oxpoconazol, paclobutrazol, uniconazol, l-(4-chloro-phenyl)-2-([l,2,4]triazol-lyl)-cycloheptanol, imazalilsulfphate, azoxystrobin, dimoxystrobin, enestroburin, fluoxastrobin, kresoxim-methyl, methominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin, enestroburin, methyl (2-chloro-5-[l-(3- methylbenzyloxyimino)ethyl]benzyl)carbamate, methyl (2-chloro-5-[l-(6- methylpyridin-2- ylmethoxyimino)ethyl]benzyl)carbamate, methyl 2-(ortho- (2,5-dimethylphenyloxymethylene)- phenyl)-3-methoxyacrylate, 2-(2-(6-(3- chloro-2-methyl-phenoxy)-5-fluoro-pyrimidin-4-yloxy)- phenyl)-2- methoxyimino-N-methyl-acetamide, 3-methoxy-2-(2-(N-(4-methoxy-phenyl)- cyclopropanecarboximidoylsulfanylmethyl)-phenyl)-acrylic acid methyl ester; carboxin, benalaxyl, benalaxyl-M, fenhexamid, flutolanil, furametpyr, mepronil, metalaxyl, mefenoxam, ofurace, oxadixyl, oxycarboxin, penthiopyrad, isopyrazam, thifluzamide, tiadinil, 3,4-dichloro-N-(2- cyanophenyl)isothiazole-5-carboxamide, dimethomorph, flumorph, flumetover, fluopicolide (picobenzamid), zoxamide, carpropamid, diclocymet, mandipropamid, N- (2-(4-[3-(4-chlorophenyl)prop-2-ynyloxy]-3- methoxyphenyl)ethyl)-2-methanesulfonyl-amino-3- methylbutyramide, N-(2- (4- [3 - (4-chloro-phenyl)prop-2-ynyloxy ] - 3 -methoxy-phenyl)ethyl)-2- ethanesulfonylamino- 3-methylbutyramide, methyl 3-(4-chlorophenyl)-3-(2- isopropoxycarbonylamino-3-methyl-butyrylamino)propionate, N-(4'- bromobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N- (4'-trifluoromethyl-biphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5- carboxamide, N-(4'- chloro-3'-fluorobiphenyl-2-yl)-4-difluoromethyl-2- methylthiazole-5-carboxamide, N-(3',4'-dichloro-4-fluorobiphenyl-2-yl)-3- difluoro-methyl-l-methylpyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-3-difluoromethyl-lmethylpyrazole-4-carboxamide, N-(2- cyano-phenyl)- 3,4-dichloroisothiazole-5-carboxamide, 2- amino-4-methyl- thiazole-5-carboxanilide, 2-chloro-N-(l,l,3-trimethyl-indan-4-yl)- nicotinamide, N- (2-(l,3-dimethylbutyl)-phenyl)-l,3-dimethyl-5-fluoro-lH- pyrazole-4-carboxamide, N-(4'-chloro3',5-difluoro-biphenyl-2-yl)-3- difluoromethyl-l-methyl-IH-pyrazole-4-carboxamide, N-(4'-chloro3',5- difluoro-biphenyl- 2-yl)-3-trifluoromethyl-l -methyl-lH-pyrazole-4- carboxamide, N-(3',4'- dichloro-5-fluoro-biphenyl-2-yl)-3-trifluoromethyl-l- methyl-lH-pyrazole-4- carboxamide, N-(3',5difluoro-4'-methyl-biphenyl-2- yl)-3-difluoromethyl- 1 -methyl-lH-pyrazole-4-carboxamide, N- (3',5- difluoro-4'-methyl-biphenyl-2-yl)-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N- (cis-2-bicyclopropyl-2-yl-phenyl)-3-difluoromethyl-l methyl-lH-pyrazole-4-carboxamide, N- (trans-2-bicyclopropyl-2-yl-phenyl)- 3-difluoro-methyl-l-methyl-l H-pyrazole-4-carboxamide, fluopyram, N-(3- ethyl-3,5-5- trimethyl-cyclohexyl)-3-formylamino-2-hydroxy-benzamide, oxytetracyclin, silthiofam, N-(6-methoxy-pyridin-3-yl) cyclopropanecarboxamide, 2-iodo-N-phenylbenzamide, N-(2-bicyclo-propyl- 2-yl-phenyl)-3-difluormethyl-l-methylpyrazol-4-ylcarboxamide, N- (3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethyl-5-fluoropyrazol-4-yl-carboxamide, N- (3',4',5'-trifluorobiphenyl-2-yl)-5-chlorol,3-dimethyl- pyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-3-fluoromethyl- lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-lmethylpyrazol-4-ylcarboxamide,N-(3',4',5'- trifluorobiphenyl-2-yl)-3 -difluoromethyl-lmethylpyrazol-4-ylcarboxamide, N - (3',4',5'-trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-lmethylpyrazol-4- ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-chloro-3-difluoromethyl-l - methylpyrazol-4-ylcarboxamide, N- (3', 4', 5'-trifluorobiphenyl-2-yl)-3- (chlorodifluoromethyl)-lmethylpyrazol-4-ylcarboxamide, N-(3',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5-fluoro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(3',4',5'-trifluorobiphenyl-2-yl)-5- chloro-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)- 1 ,3-dimethyl-5-fluoropyrazol-4-ylcarboxamide, N- (2, ,4 , ,5'- trifluorobiphenyl-2-yl)-5-chloro-l,3-dimethylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-fluoromethyl-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3- (chlorofluoromethyl)-l-methylpyrazol-4-ylcarboxamide,N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-3-difluoromethyl-5-fluoro-l- methylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2- yl)-5-chloro- 3-difluoromethyl-l-methylpyrazol-4-ylcarboxamide, N- (2',4',5'- trifluorobiphenyl-2-yl)-3-(chlorodifluoromethyl)-l-methylpyrazol-4- ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-l-methyl-3- trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5 trifluorobiphenyl-2-yl)- 5-fluoro-l-methyl-3-trifluoromethylpyrazol-4-ylcarboxamide, N-(2',4',5'- trifluorobiphenyl-2-yl)-5-chloro-l-methyl-3-trifluoromethylpyrazol-4- ylcarboxamide, N-(3',4'- dichloro-3-fluorobiphenyl-2-yl)-l -methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'- dichloro-3- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-l H-pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'- difluoro-3-fluorobiphenyl-2-yl)-l-methyl- S-difluoromethyl-1 H-pyrazole-4-carboxamide, N-(3'- chloro-4'-fluoro-3- fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3M'-dichloro-4-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-difluoro-4-fluorobiphenyl-2-yl)-l - methyl- S-trifluoromethyl-lH-pyrazole-4-carboxamide, N- (3 ',4'-dichloro-4- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4- carboxamide, N- (3M'-difluoro-4-fluorobiphenyl-2-yl)-l-methyl-3-difluoromethyl-lH- pyrazole-4-carboxamide, N- (3'-chloro-4'-fluoro-4-fluorobiphenyl-2-yl)-l- methyl-5-difluoromethyl-lH-pyrazole-4-carboxamide, N-(3',4'-dichloro-5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide, N-(3',4'-difluoro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH- pyrazole-4-carboxamide, N-(3',4'-dichloro-5-fluorobiphenyl-2-yl)-l - methyl- S-difluoromethyl-IH-pyrazole-carboxamide, N- (3',4'-difluoro-5- fluorobiphenyl-2-yl)-l -methyl-3-difluoromethyl-lH-pyrazole-4-carboxamide, N- (3',4'-dichloro-5-fluorobiphenyl-2-yl)-l,3-dimethyl-l H-pyrazole-4- carboxamide, N-(3'-chloro-4'- fluoro-5-fluorobiphenyl-2-yl)-l-methyl-3- difluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-4-fluorobiphenyl-2- yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'-fluoro- 5- fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl-lH-pyrazole-4- carboxamide,N-(4'- chloro-5-fluorobiphenyl-2-yl)-l-methyl-3-trifluoromethyl- lH-pyrazole-4-carboxamide, N-(4'- methyl-5-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-5-fluorobiphenyl- 2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-chloro-5- fluorobiphenyl-2-yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'-methyl- 5-fluorobiphenyl-2- yl)-l,3-dimethyl-lH-pyrazole-4-carboxamide, N-(4'- fluoro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4- carboxamide, N-(4'-chloro-6-fluorobiphenyl-2-yl)-l-methyl-3- trifluoromethyl-lH-pyrazole-4-carboxamide, N-[2-(l, 1,2, 3,3,3- hexafluoropropoxy)-phenyl]-3- difluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, N-[4'-(trifluoromethylthio)-biphenyl-2-yl]- 3-difluoromethyl-l- methyl-lH-pyrazole-4-carboxamide and N-[4'-(trifluoromethylthio)- biphenyl2-yl]-l-methyl-3-trifluoromethyl-l-methyl-lH-pyrazole-4- carboxamide, fluazinam, pyrifenox, bupirimate, cyprodinil, fenarimol, ferimzone, mepanipyrim, nuarimol, pyrimethanil, triforine, fenpiclonil, fludioxonil, aldimorph, dodemorph, fenpropimorph, tridemorph, fenpropidin, iprodione, procymidone, vinclozolin, famoxadone, fenamidone, octhilinone, probenazole, 5-chloro-7-(4-methyl-piperidin-l-yl)-6-(2,4,6- trifluorophenyl)-
[1.2.4]triazolo[l,5-a]pyrimidine, anilazine, diclomezine, pyroquilon, proquinazid, tricyclazole, 2-butoxy-6-iodo-3-propylchromen-4-one, acibenzolar-S-methyl, captafol, captan, dazomet, folpet, fenoxanil, quinoxyfen, N,N-dimethyl-3-(3-bromo-6-fluoro-2-methylindole-lsulfonyl)-
[1.2.4]triazole-l-sulfonamide, 5-ethyl-6-octyl-[l,2,4]triazolo[l,5- a]pyrimidin- 2,7-diamine, 2,3,5,6-tetrachloro-4-methanesulfonyl-pyridine, 3,4,5-trichloro- pyridine-2,6-di-carbonitrile, N-(l- (5-bromo-3-chloro-pyridin-2-yl)-ethyl)-2,4- dichloro-nicotinamide, N-((5-bromo-3-chloropyridin-2- yl)-methyl)-2,4- dichloro-nicotinamide, diflumetorim, nitrapyrin, dodemorphacetate, fluoroimid, blasticidin-S, chinomethionat, debacarb, difenzoquat, difenzoquat-methylsulphat, oxolinic acid, piperalin, mancozeb, maneb, metam, methasulphocarb, medram, ferbam, propineb, thiram, zineb, ziram, diethofencarb, iprovalicarb, benthiavalicarb, propamocarb, propamocarb hydrochlorid, 4-fluorophenyl N-(l-(l-(4-cyanophenyl)-ethanesulfonyl)but-2- yl)carbamate, methyl 3-(4-chloro-phenyl)-3-(2- isopropoxycarbonylamino-3- methylbutyrylamino)propanoate, guanidine, iminoctadine, guazatine, kasugamycin, oxytetracyclin, streptomycin, polyoxin, validamycin, binapacryl, dinocap, dinobuton, sulfur-containing heterocyclyl compounds: dithianon, isoprothiolane, edifenphos, iprobenfos, fosetyl, fosetyl-aluminum, pyrazophos, tolclofos- methyl, dichlofluanid, flusulfamide, hexachloro- benzene, phthalide, pencycuron, quintozene, thiophanate, thiophanatemethyl, cyflufenamid, cymoxanil, dimethirimol, ethirimol, furalaxyl, metrafenone and spiroxamine, guazatine-acetate, iminoc- tadine-triacetate, iminoctadinetris(albesilate), kasugamycin hydrochloride hydrate, dichlorophen, pentachlorophenol and its salts, N-(4- chloro-2-nitro-phenyl)- N-ethyl-4-methyl-benzenesulfonamide, dicloran, nitrothal-isopropyl, tecnazen, biphenyl, bronopol, diphenylamine, mildiomycin, oxincopper, prohexadione calcium, N - (cyclopropylmethoxyimino- (6-difluoromethoxy- 2,3-difluoro-phenyl)-methyl)-2-ph acetamide, N'-(4-(4-chloro-3- trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl
formamidine, N'-(4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl- phenyl)-N-ethyl-N-methyl formamidine, N'-(2-methyl-5-trifluormethyl-4-(3- trimethylsilanyl-propoxy)-phenyl)-N-ethyl-Nmethylformamidine and N'-(5- difluormethyl-2-methyl- 4-(3-trimethylsilanyl-propoxy)-phenyl)-Nethyl-N- methyl formamidine. c) Herbicides: alloxydim, clethodim, cloproxydim, cycloxydim, sethoxydim, tralkoxydim, butroxydim, clefoxydim, tepraloxydim; phenoxyphenoxypropionic esters, clodinafop-propargyl, cyhalofop-butyl, diclofop-methyl, fenoxa prop-ethyl, fenoxaprop-P-ethyl, fenthiapropethyl, fluazifop-butyl, fluazifop-P-butyl, haloxyfop-ethoxyethyl, haloxyfop-methyl, haloxyfop-P-methyl, isoxapyrifop, propaquizafop, quizalofop-ethyl, quizalofop-P-ethyl o r quizalofop-tefuryl, flamprop-methyl, flamprop-isopropyl, imazapyr, imazaquin, imazamethabenz-methyl
(imazame), imazamox, imazapic, imazethapyr, pyrithiobac-acid, pyrithiobac- sodium, bispyribac-sodium, pyribenzoxym, florasulam, flumetsulam, metosulam, amidosulfuron, azimsulfuron, bensulfuron-methyl, chlorimuron- ethyl, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron-methyl, ethoxysulfuron, flazasulfuron, halosulfuron-methyl, imazosulfuron, metsulfuron-methyl, nicosulfuron, primisulfuron-methyl, prosulfuron, pyrazosulfuron-ethyl, rimsulfuron, sulfometuron-methyl, thifensulfuron- methyl, triasulfuron, tribenuron-methyl, triflusulfuron-methyl, tritosulfuron, sulfosulfuron, foramsulfuron, iodosulfuron, benzoyl prop-ethyl, bromobutide, chiorthiamid, diphenamid, etobenzanidibenzchlomet, fluthiamide, fosamin, monalide, clopyralid, picloram, 2,4-D, benazolin, naptalame, diflufenzopyr, benzofenap, clomazone, diflufenican, fluorochloridone, fluridone, pyrazolynate, pyrazoxyfen, isoxaflutole, isoxachlortole, mesotrione, sulcotrione, ketospiradox, flurtamone, norflurazon, amitrol, glyphosate, sulfosate, bilanafos, glufosinateammonium, anilofos, mefenacet, dimethenamid, S-dimethenamid, acetochlor, alachlor, butachlor, butenachlor, diethatyl-ethyl, dimethachlor, metazachlor, metolachlor, S-metolachlor, pretilachlor, propachlor, prynachlor, terbuchlor, thenylchlor, xylachlor, butylate, cycloate, di-allate, dimepiperate, EPTC. esprocarb, molinate, pebulate, prosulfocarb, thiobencarb, tri-allate, vemolate; benfuresate, perfluidone; asulam, carbetamid, chlorpropham, orbencarb, pronamid, propham, tiocarbazil benefin, butralin, dinitramin, ethalfluralin, fluchloralin, oryzalin, pendimethalin, prodiamine, trifluralin dithiopyr, thiazopyr, butamifos, chlorthal-dimethyl (DCPA), acifluorfen, acifluorfen-sodium, aclonifen, bifenox, chlomitrofen (CNP), ethoxyfen, fluorodifen, fluoroglycof enethyl, fomesafen, furyloxyfen, lactofen, nitrofen, nitrofluorfen or oxyfluorfen, oxadiargyl, oxadiazon, azafenidin, butafenacil, carfentrazone- ethyl, cinidon-ethyl, flumiclorac-pentyl, flumioxazin, flumipropyn, flupropacil, fluthiacet-methyl, sulfentrazone, thidiazimin, nipyraclofen, propanil, pyridate or pyridafol, bromofenoxim, dinoseb, dinoseb-acetate, dinoterb, cyperquat-chloride, difenzoquat-methylsulfate, diquat or paraquatdichloride, chlorbromuron, chlorotoluron, difenoxuron, dimefuron, diuron, ethidimuron, fenuron, fluometuron, isoproturon, isouron, linuron, methabenzthiazuron, methazole, metobenzuron, metoxuron, monolinuron, neburon, siduron, tebuthiuron, bromoxynil, ioxynil, chloridazon, ametryn, atrazine, cyanazine, desmein, dimethamethryn, hexazinone, prometon, prometryn, propazine, simazine, simetryn, terbumeton, terbutryn, terbutylazine, trietazine, metamitron, metribuzin bromacil, lenacil, terbacil, desmedipham, phenmedipham, tridiphane, isoxaben, dichlobenil, dalapon, ethofumesate, chlorfenac, aziprotryn, barban, bensulide, benzthiazuron, benzofluor, buminafos, buthidazole, buturon, cafenstrole, chlorbufam, chlorfenprop-methyl, chloroxuron, cinmethylin, cumyluron, cycluron, cyprazine, cyprazole, dibenzyluron, dipropetryn, dymron, eglinazin-ethyl, endothall, ethiozin, flucabazone, fluorbentranil, flupoxam, isocarbamid, isopropalin, karbutilate, mefluidide, monuron, napropamide, napropanilide, nitralin, oxaciclomefone, phenisopham, piperophos, procyazine, profluralin, pyributicarb, secbumeton, sulfallate (CDEC), terbucarb, triaziflam, triazofenamid, trimeturon, dentre outros d) Nematicidas: Benomyl, cloethocarb, aldoxycarb, tirpate, diamidafos, fenamiphos, cadusafos, dichlofenthion, ethoprophos, fensulfothion, fosthiazate, heterophos, isamidofof, isazofos, phosphocarb, thionazin, imicyafos, mecarphon, acetoprole, benclothiaz, chloropicrin, dazomet, fluensulfone, 1,3- dichloropropene (telone), dimethyl disulfide, metam sodium, metam potassium, metam salt (all MITC generators), methyl bromide, steam fumigation of soil, allyl isothiocyanate (AITC), dimethyl sulfate, furfual (aldehyde), dentre outros.
9 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos aditivos serem dispersantes selecionados do grupo consistindo de polímeros iônicos solúveis em água, polímeros aniônicos solúveis em água e surfactantes selecionados do grupo consistindo de surfactantes aniônicos e surfactantes não iônicos e combinações entre eles.
10 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado pelos excipientes serem do grupo que consiste em sílicas, talco, bentonita, carboidratos, carbonatos, derivados do leite (soro e leite em pó) e combinações entre eles.
11 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada como formulação em pó molhável, emulsões, microemulsões, suspensões, suspensões concentradas, grânulos, encapsulamento, microencapsulamento e nanoencapsulamento.
12 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1 , caracterizado pelos por ser empregada como pulverização de sulco de plantio, drench, no revestimento (industrial ou não), tratamento (industrial ou não) e incrustação (industrial ou não) de sementes das plantas cultivadas, não se limitando a Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.j, Nicotiana tabacum, ornamentais, frutíferas 0 Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis), crotalárias. ( Crotalaria juncea, Crotalária spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora).
13 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada no controle de fitopatógenos, tais como: Phakopsora pachyrhizi, Corynespora cassiicola, Puccinia polysora, Exserohilum turcicum, Xanthomonas axonopodis, Ramularia areola, Hemileia vastatrix, Cercospora coffeicola, Phoma spp., Pseudomonas syringae, Rhizoctonia solani, Fusarium solani, Fusarium oxysporum, Fusarium graminearum, Fusarium semitectum, Colletotrichum gloeosporioides, Colletotrichum truncatum, Colletotrichum lindemuthianum, Macrophomna phaseolina, Sclerotinia sclerotiorum, Botrytis cinerea, Penicillium digitatum.
14 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada no controle de fitonematóides, tais como: Pratylenchus brachyurus, Pratylenchus zea, Meloidogyne incógnita, Meloidogyne javanica, Heterodera glycines, Rotylenchulus reniformis, Pratylenchus coffea, Meloidogyne exigua, Helicotilechus dihystera.
15 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada no controle de pragas agrícolas, tais como: Polyphagotarsonemus latus, Tetranychus urticae, Diabrotica speciosa, Anticarsia gemmatalis, Spodoptera cosmioides, Spodoptera frugiperda, Pseudoplusia includens, Trichoplusia ni, Bemisia tabaci, Euschistos heros, Nezara viridula, Leucoptera coffeella, Hypothenemus hampei, Oligonychus ilicis, Polyphagotarsonemus latus , Brevipalpus phoenicis, Planococcus citri, Planococcus minor.
16 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de bioestimulantes, inoculantes, solubilizador de fósforo e estimulantes de crescimento nas plantas cultivadas, tais como: Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta ), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.j, Nicotiana tabacum, ornamentais, frutíferas 0 Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis), crotalárias. (Crotalaria juncea, Crotalária spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora). 17 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de condicionador de solos para todas as culturas e qualquer área agriculturável.
18 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de mitigar ou reduzir o estresse hídrico ou abiótico nas plantas cultivadas, tais como: Coffea spp ( Coffea arabica, Coffea canefora, Coffea robusta), Brachiaria spp. (Brachiaria brizantha, Brachiaria decumbens, Brachiaria humidicola, Brachiaria mutica, Brachiaria ruziziensis, Brachiaria arrecta, Brachiaria dictyneura), Panicum spp. (Panicum maximum), Sorghum spp., Pennisetum americanum, Glycine max, Zea mays, Gossypium hirsutum, hortaliças (Solanum lycopersicum, Allium cepa, Lactuca sativa, Daucus carota, Capsicum annuum Group), florestais (Eucalyptus spp., Tectona grandis, Hevea brasiliensis, Pinus sp.j, Nicotiana tabacum, ornamentais, frutíferas ( Citrus spp., Malus spp., Carica papaya, Vitis spp., Cucumis melo, Prunus pérsica, Passiflora edulis), crotalárias. ( Crotalaria juncea, Crotalária spectabilis, Crotalaria ochroleuca, Crotalaria paulina, Crotalaria breviflora).
19 - COMPOSIÇÃO BIOLÓGICA DE FUNÇÕES _
MÚLTIPLAS, de acordo com a reivindicação 1, caracterizado por ser empregada com a função de biorremediador de áreas agricultáveis ou não para a redução de contaminação ambiental por resíduos tóxicos.
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